Afg3l2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Afg3l2
Entrez Gene ID	69597
Full Name	AFG3-like AAA ATPase 2
Synonyms	2310036I02Rik,AW260507,Emv66,par
General protein information	Preferred Names AFG3-like AAA ATPase 2 Names AFG3-like protein 2 AFG3(ATPase family gene 3)-like 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011246998.2	XP_011245300.1	AFG3-like protein 2 isoform X1
NM_027130.2	NP_081406.1	AFG3-like protein 2
NM_027130.1	NP_081406.1	AFG3-like protein 2
XM_011246998.1	XP_011245300.1	AFG3-like protein 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	AFG3L2	NP_006787.2
Pan troglodytes (chimpanzee)	AFG3L2	XP_512199.2
Saccharomyces cerevisiae (baker's yeast)	AFG3	NP_010933.1
Eremothecium gossypii	AGOS_AAR025C	NP_982566.2
Arabidopsis thaliana (thale cress)	ftsh10	NP_172231.2
Bos taurus (cattle)	AFG3L2	NP_001039676.1
Mus musculus (house mouse)	Afg3l2	NP_081406.1
Arabidopsis thaliana (thale cress)	ftsh3	NP_850129.1
	AFG3L2	XP_001094146.1
Gallus gallus (chicken)	AFG3L2	XP_419121.2
Drosophila melanogaster (fruit fly)	CG6512	NP_730248.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006949	XP_308807.4
Caenorhabditis elegans (roundworm)	spg-7	NP_491165.2
Danio rerio (zebrafish)	afg3l2	NP_001104667.1
Oryza sativa (rice)	Os05g0458400	NP_001055745.1
Canis lupus familiaris (dog)	AFG3L2	XP_547682.2
Rattus norvegicus (Norway rat)	Afg3l2	NP_001128336.1
Oryza sativa (rice)	Os01g0842600	NP_001044774.1
Xenopus tropicalis (tropical clawed frog)	afg3l2	NP_001072759.1

Gene Ontology
Bibliography

Related articles in PubMed

The Mitochondrial m-AAA Protease Prevents Demyelination and Hair Greying.
Wang S, Jacquemyn J, Murru S, Martinelli P, Barth E, Langer T, Niessen CM, Rugarli EI
PLoS genetics12(12)e1006463(2016 Dec)

The m-AAA Protease Associated with Neurodegeneration Limits MCU Activity in Mitochondria.
K?nig T, Tr?der SE, Bakka K, Korwitz A, Richter-Dennerlein R, Lampe PA, Patron M, M?hlmeister M, Guerrero-Castillo S, Brandt U, Decker T, Lauria I, Paggio A, Rizzuto R, Rugarli EI, De Stefani D, Langer T
Molecular cell64(1)148-162(2016 Oct)

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura A, Kaitsuka T, Takeya M, Oike Y, Suzuki T, Tomizawa K
PLoS genetics12(9)e1006355(2016 Sep)

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.
Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ
The Journal of cell biology211(2)373-89(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

total ablation of the m-AAA protease, by deleting both Afg3l2 and its paralogue Afg3l1, triggers progressive motor dysfunction and demyelination, owing to rapid oligodendrocyte cell death
Title: The Mitochondrial m-AAA Protease Prevents Demyelination and Hair Greying.

AFG3L2 is critical to the surveillance mechanism that acts as a sensor to couple the synthesis of mitochondrial proteins with organelle fitness, thus ensuring coordinated assembly of the oxidative phosphorylation complexes from two sets of ribosomes.
Title: Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Reactive oxygen species signaling leads to cytoskeletal modifications that impair mitochondrial transport in neurons lacking AFGL.
Title: Loss of the m-AAA protease subunit AFG?L? causes mitochondrial transport defects and tau hyperphosphorylation.

defective mitochondrial protein synthesis, leading to early-onset fragmentation of the mitochondrial network, is a central causative factor in AFG3L2-related neurodegeneration.
Title: AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.

These findings shed new light in the molecular mechanisms underlining neurodegeneration caused by AFG3L2 mutations.
Title: Respiratory dysfunction by AFG3L2 deficiency causes decreased mitochondrial calcium uptake via organellar network fragmentation.

The following Afg3l2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afg3l2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23383	NM_027130.2 Latest version!	Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$468.30 $669.00
OMu70436	XM_011246998.2 Latest version!	Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu23383	NM_027130.1	Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$468.30 $669.00
OMu70436	XM_011246998.1	Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Afg3l2 ( XM_011246998.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245300.1 Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70436
Clone ID Related Accession (Same CDS sequence)	XM_011246998.2 , XM_011246998.1
Accession Version	XM_011246998.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AFG3-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA CGGAAAAAAG GCCAGTGAGC CTAAGGAGGC TGTTGGAGAA AAAAAAAGAA 
CCACAGCCCT CGGGCCCCCA GCCTTCTGGA GGTGCAGGTG GTGGGGGAGG GAAGCGCCGT 
GGCAAGAAAG AAGATTCTCA CTGGTGGTCC AGGTTCCAGA AGGGTGACTT CCCATGGGAT 
GACAAGGATT TCAGGATGTA CTTTCTCTGG ACTGCTCTTT TTTGGGGTGG AGTCATGATT 
TACTTCGTGT TCAAGAGCTC TGGGAGAGAA ATCACGTGGA AAGACTTTGT CAATAACTAT 
CTTTCTAAGG GCGTGGTGGA CAGACTAGAA GTTGTCAACA AGCGTTTTGT TCGTGTGACC 
TTTACACCAG GAAAAACTCC GGTTGATGGG CAATACGTCT GGTTTAATAT TGGCAGTGTT 
GACACATTTG AGCGGAATCT GGAGACTTTG CAGCAAGAAT TGGGCATAGA AGGGGAGAAC 
CGGGTCCCTG TGGTTTATAT TGCTGAGAGC GATGGCTCCT TCCTGCTGAG CATGTTGCCC 
ACCGTACTCA TTATCGCTTT TCTACTCTAC ACCATAAGAA GAGGGCCCGC TGGCATTGGT 
CGGACCGGCC GGGGAATGGG TGGACTCTTC AGCGTTGGGG AAACCACAGC CAAGGTCTTA 
AAGGATGAGA TAGATGTGAA GTTTAAAGAT GTGGCTGGCT GTGAGGAGGC CAAGCTAGAA 
ATAATGGAAT TCGTGAATTT CTTGAAAAAC CCAAAGCAAT ATCAAGACCT AGGAGCAAAA 
ATCCCAAAGG GTGCCATTCT CACCGGTCCC CCAGGTACTG GGAAGACGCT GCTAGCTAAG 
GCCACAGCTG GAGAAGCCAA TGTCCCCTTT ATCACTGTGA GCGGATCTGA GTTTCTGGAG 
ATGTTTGTTG GCGTTGGTCC AGCCAGAGTC CGAGACTTAT TTGCCCTTGC TCGGAAGAAT 
GCGCCTTGCA TTCTCTTCAT TGATGAGATT GATGCTGTGG GAAGGAAGCG CGGCAGAGGC 
AACTTCGGTG GGCAGAGCGA GCAGGAGAAC ACACTCAACC AGCTGCTTGT GGAGATGGAC 
GGCTTCAACA CAACCACCAA TGTGGTCATC CTGGCAGGCA CAAATCGACC AGACATCCTG 
GATCCAGCTC TGTTGAGACC AGGCCGCTTT GACAGGCAGA TTTTTATTGG ACCCCCAGAC 
ATAAAAGGAC GAGCCTCAAT CTTCAAAGTT CACCTTCGAC CATTGAAGCT GGACAGTGCC 
TTGGAAAAAG ATAAATTGGC CAGAAAACTG GCGTCCTTAA CTCCAGGGTT TTCAGGCGCT 
GATGTTGCCA ATGTCTGCAA TGAAGCTGCT TTGATTGCTG CAAGACACCT TTCAGATGCC 
ATTAATGAGA AGCACTTCGA ACAAGCGATT GAGCGAGTGA TTGGAGGCTT GGAGAAAAAA 
ACCCAAGTTC TGCAGCCTGA GGAGAAGAAG ACGGTGGCTT ACCACGAAGC AGGCCATGCG 
GTCGCTGGCT GGTATCTGGA GCATGCAGAC CCACTCTTAA AGGTCTCCAT CATCCCGCGT 
GGCAAGGGGC TGGGCTATGC TCAGTACTTG CCCAAGGAGC AGTATCTGTA CACAAAGGAG 
CAGCTGCTGG ACAGGATGTG CATGACTCTG GGCGGCCGTG TCTCCGAGGA GATCTTCTTT 
GGGAGAATTA CAACCGGTGC CCAGGACGAC TTGAGGAAGG TTACCCAGAG TGCCTATGCC 
CAGATCGTTC AGTTTGGCAT GAACGAGAAA GTGGGGCAGA TCTCCTTTGA CCTCCCACGA 
CAGGGGGACA TGGTGTTAGA GAAGCCTTAC AGTGAGGCCA CTGCGAGGAT GATAGACGAT 
GAAGTGAGGA TACTCATCAG CGATGCCTAC AGAAGGACGG TGGCTCTTCT CACAGAGAAG 
AAGGCTGACG TGGAGAAGGT CGCTCTCTTA CTGTTAGAAA AGGAAGTCCT AGACAAGAAT 
GACATGGTCC AGCTTCTCGG TCCCAGACCA TTTACAGAAA AGTCCACATA TGAAGAATTT 
GTGGAAGGCA CTGGCAGCTT AGACGAGGAC ACTTCTCTTC CTGAAGGCCT TCAGGATTGG 
AACAAGGAGC GGGAGAAGGA GGAGAAGAAG GAGAAGGAGA AGGAGGAGCC GCTGAATGAG 
AAGGTTGTCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245300.1
CDS	1435..3609
Translation	MGKTEKRPVSLRRLLEKKKEPQPSGPQPSGGAGGGGGKRRGKKEDSHWWSRFQKGDFPWDDKDFRMYFLWTALFWGGVMIYFVFKSSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDGSFLLSMLPTVLIIAFLLYTIRRGPAGIGRTGRGMGGLFSVGETTAKVLKDEIDVKFKDVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAILTGPPGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRGNFGGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSALEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDAINEKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARMIDDEVRILISDAYRRTVALLTEKKADVEKVALLLLEKEVLDKNDMVQLLGPRPFTEKSTYEEFVEGTGSLDEDTSLPEGLQDWNKEREKEEKKEKEKEEPLNEKVVS

Target ORF information:

RefSeq Version	XM_011246998.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246998.1

ORF Insert Sequence:

ATGGGAAAAA CGGAAAAAAG GCCAGTGAGC CTAAGGAGGC TGTTGGAGAA AAAAAAAGAA 
CCACAGCCCT CGGGCCCCCA GCCTTCTGGA GGTGCAGGTG GTGGGGGAGG GAAGCGCCGT 
GGCAAGAAAG AAGATTCTCA CTGGTGGTCC AGGTTCCAGA AGGGTGACTT CCCATGGGAT 
GACAAGGATT TCAGGATGTA CTTTCTCTGG ACTGCTCTTT TTTGGGGTGG AGTCATGATT 
TACTTCGTGT TCAAGAGCTC TGGGAGAGAA ATCACGTGGA AAGACTTTGT CAATAACTAT 
CTTTCTAAGG GCGTGGTGGA CAGACTAGAA GTTGTCAACA AGCGTTTTGT TCGTGTGACC 
TTTACACCAG GAAAAACTCC GGTTGATGGG CAATACGTCT GGTTTAATAT TGGCAGTGTT 
GACACATTTG AGCGGAATCT GGAGACTTTG CAGCAAGAAT TGGGCATAGA AGGGGAGAAC 
CGGGTCCCTG TGGTTTATAT TGCTGAGAGC GATGGCTCCT TCCTGCTGAG CATGTTGCCC 
ACCGTACTCA TTATCGCTTT TCTACTCTAC ACCATAAGAA GAGGGCCCGC TGGCATTGGT 
CGGACCGGCC GGGGAATGGG TGGACTCTTC AGCGTTGGGG AAACCACAGC CAAGGTCTTA 
AAGGATGAGA TAGATGTGAA GTTTAAAGAT GTGGCTGGCT GTGAGGAGGC CAAGCTAGAA 
ATAATGGAAT TCGTGAATTT CTTGAAAAAC CCAAAGCAAT ATCAAGACCT AGGAGCAAAA 
ATCCCAAAGG GTGCCATTCT CACCGGTCCC CCAGGTACTG GGAAGACGCT GCTAGCTAAG 
GCCACAGCTG GAGAAGCCAA TGTCCCCTTT ATCACTGTGA GCGGATCTGA GTTTCTGGAG 
ATGTTTGTTG GCGTTGGTCC AGCCAGAGTC CGAGACTTAT TTGCCCTTGC TCGGAAGAAT 
GCGCCTTGCA TTCTCTTCAT TGATGAGATT GATGCTGTGG GAAGGAAGCG CGGCAGAGGC 
AACTTCGGTG GGCAGAGCGA GCAGGAGAAC ACACTCAACC AGCTGCTTGT GGAGATGGAC 
GGCTTCAACA CAACCACCAA TGTGGTCATC CTGGCAGGCA CAAATCGACC AGACATCCTG 
GATCCAGCTC TGTTGAGACC AGGCCGCTTT GACAGGCAGA TTTTTATTGG ACCCCCAGAC 
ATAAAAGGAC GAGCCTCAAT CTTCAAAGTT CACCTTCGAC CATTGAAGCT GGACAGTGCC 
TTGGAAAAAG ATAAATTGGC CAGAAAACTG GCGTCCTTAA CTCCAGGGTT TTCAGGCGCT 
GATGTTGCCA ATGTCTGCAA TGAAGCTGCT TTGATTGCTG CAAGACACCT TTCAGATGCC 
ATTAATGAGA AGCACTTCGA ACAAGCGATT GAGCGAGTGA TTGGAGGCTT GGAGAAAAAA 
ACCCAAGTTC TGCAGCCTGA GGAGAAGAAG ACGGTGGCTT ACCACGAAGC AGGCCATGCG 
GTCGCTGGCT GGTATCTGGA GCATGCAGAC CCACTCTTAA AGGTCTCCAT CATCCCGCGT 
GGCAAGGGGC TGGGCTATGC TCAGTACTTG CCCAAGGAGC AGTATCTGTA CACAAAGGAG 
CAGCTGCTGG ACAGGATGTG CATGACTCTG GGCGGCCGTG TCTCCGAGGA GATCTTCTTT 
GGGAGAATTA CAACCGGTGC CCAGGACGAC TTGAGGAAGG TTACCCAGAG TGCCTATGCC 
CAGATCGTTC AGTTTGGCAT GAACGAGAAA GTGGGGCAGA TCTCCTTTGA CCTCCCACGA 
CAGGGGGACA TGGTGTTAGA GAAGCCTTAC AGTGAGGCCA CTGCGAGGAT GATAGACGAT 
GAAGTGAGGA TACTCATCAG CGATGCCTAC AGAAGGACGG TGGCTCTTCT CACAGAGAAG 
AAGGCTGACG TGGAGAAGGT CGCTCTCTTA CTGTTAGAAA AGGAAGTCCT AGACAAGAAT 
GACATGGTCC AGCTTCTCGG TCCCAGACCA TTTACAGAAA AGTCCACATA TGAAGAATTT 
GTGGAAGGCA CTGGCAGCTT AGACGAGGAC ACTTCTCTTC CTGAAGGCCT TCAGGATTGG 
AACAAGGAGC GGGAGAAGGA GGAGAAGAAG GAGAAGGAGA AGGAGGAGCC GCTGAATGAG 
AAGGTTGTCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afg3l2 ( NM_027130.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081406.1 Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), mRNA.

Price & Availability↑

CloneID	OMu23383
Clone ID Related Accession (Same CDS sequence)	NM_027130.2 , NM_027130.1
Accession Version	NM_027130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	AFG3-like protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK168668.1 and BC043056.1. On Jul 21, 2018 this sequence version replaced NM_027130.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043056.1, SRR7345562.757211.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCACC GCTGCCTGCT GCTGTGGAGC CGGGGCGGCT GCCGTCGCGG CCTTCCTCCC 
CTGCTCGTGC CCAGAGGTTG CCTGGGTCCG GACCGGCGGC CCTGCCTCCG TACGCTCTAT 
CAATATGCTA CTGTCCAGAC AGCAAGCAGC AGGCGTTCTC TGCTGAGGGA TGTAATTGCT 
GCTTATCAAA GATTCTGTTC TCGACCTCCC AAAGGATTTG AAAAGTACTT TCCTAATGGG 
AAAAACGGAA AAAAGGCCAG TGAGCCTAAG GAGGCTGTTG GAGAAAAAAA AGAACCACAG 
CCCTCGGGCC CCCAGCCTTC TGGAGGTGCA GGTGGTGGGG GAGGGAAGCG CCGTGGCAAG 
AAAGAAGATT CTCACTGGTG GTCCAGGTTC CAGAAGGGTG ACTTCCCATG GGATGACAAG 
GATTTCAGGA TGTACTTTCT CTGGACTGCT CTTTTTTGGG GTGGAGTCAT GATTTACTTC 
GTGTTCAAGA GCTCTGGGAG AGAAATCACG TGGAAAGACT TTGTCAATAA CTATCTTTCT 
AAGGGCGTGG TGGACAGACT AGAAGTTGTC AACAAGCGTT TTGTTCGTGT GACCTTTACA 
CCAGGAAAAA CTCCGGTTGA TGGGCAATAC GTCTGGTTTA ATATTGGCAG TGTTGACACA 
TTTGAGCGGA ATCTGGAGAC TTTGCAGCAA GAATTGGGCA TAGAAGGGGA GAACCGGGTC 
CCTGTGGTTT ATATTGCTGA GAGCGATGGC TCCTTCCTGC TGAGCATGTT GCCCACCGTA 
CTCATTATCG CTTTTCTACT CTACACCATA AGAAGAGGGC CCGCTGGCAT TGGTCGGACC 
GGCCGGGGAA TGGGTGGACT CTTCAGCGTT GGGGAAACCA CAGCCAAGGT CTTAAAGGAT 
GAGATAGATG TGAAGTTTAA AGATGTGGCT GGCTGTGAGG AGGCCAAGCT AGAAATAATG 
GAATTCGTGA ATTTCTTGAA AAACCCAAAG CAATATCAAG ACCTAGGAGC AAAAATCCCA 
AAGGGTGCCA TTCTCACCGG TCCCCCAGGT ACTGGGAAGA CGCTGCTAGC TAAGGCCACA 
GCTGGAGAAG CCAATGTCCC CTTTATCACT GTGAGCGGAT CTGAGTTTCT GGAGATGTTT 
GTTGGCGTTG GTCCAGCCAG AGTCCGAGAC TTATTTGCCC TTGCTCGGAA GAATGCGCCT 
TGCATTCTCT TCATTGATGA GATTGATGCT GTGGGAAGGA AGCGCGGCAG AGGCAACTTC 
GGTGGGCAGA GCGAGCAGGA GAACACACTC AACCAGCTGC TTGTGGAGAT GGACGGCTTC 
AACACAACCA CCAATGTGGT CATCCTGGCA GGCACAAATC GACCAGACAT CCTGGATCCA 
GCTCTGTTGA GACCAGGCCG CTTTGACAGG CAGATTTTTA TTGGACCCCC AGACATAAAA 
GGACGAGCCT CAATCTTCAA AGTTCACCTT CGACCATTGA AGCTGGACAG TGCCTTGGAA 
AAAGATAAAT TGGCCAGAAA ACTGGCGTCC TTAACTCCAG GGTTTTCAGG CGCTGATGTT 
GCCAATGTCT GCAATGAAGC TGCTTTGATT GCTGCAAGAC ACCTTTCAGA TGCCATTAAT 
GAGAAGCACT TCGAACAAGC GATTGAGCGA GTGATTGGAG GCTTGGAGAA AAAAACCCAA 
GTTCTGCAGC CTGAGGAGAA GAAGACGGTG GCTTACCACG AAGCAGGCCA TGCGGTCGCT 
GGCTGGTATC TGGAGCATGC AGACCCACTC TTAAAGGTCT CCATCATCCC GCGTGGCAAG 
GGGCTGGGCT ATGCTCAGTA CTTGCCCAAG GAGCAGTATC TGTACACAAA GGAGCAGCTG 
CTGGACAGGA TGTGCATGAC TCTGGGCGGC CGTGTCTCCG AGGAGATCTT CTTTGGGAGA 
ATTACAACCG GTGCCCAGGA CGACTTGAGG AAGGTTACCC AGAGTGCCTA TGCCCAGATC 
GTTCAGTTTG GCATGAACGA GAAAGTGGGG CAGATCTCCT TTGACCTCCC ACGACAGGGG 
GACATGGTGT TAGAGAAGCC TTACAGTGAG GCCACTGCGA GGATGATAGA CGATGAAGTG 
AGGATACTCA TCAGCGATGC CTACAGAAGG ACGGTGGCTC TTCTCACAGA GAAGAAGGCT 
GACGTGGAGA AGGTCGCTCT CTTACTGTTA GAAAAGGAAG TCCTAGACAA GAATGACATG 
GTCCAGCTTC TCGGTCCCAG ACCATTTACA GAAAAGTCCA CATATGAAGA ATTTGTGGAA 
GGCACTGGCA GCTTAGACGA GGACACTTCT CTTCCTGAAG GCCTTCAGGA TTGGAACAAG 
GAGCGGGAGA AGGAGGAGAA GAAGGAGAAG GAGAAGGAGG AGCCGCTGAA TGAGAAGGTT 
GTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081406.1
CDS	192..2600
Translation	MAHRCLLLWSRGGCRRGLPPLLVPRGCLGPDRRPCLRTLYQYATVQTASSRRSLLRDVIAAYQRFCSRPPKGFEKYFPNGKNGKKASEPKEAVGEKKEPQPSGPQPSGGAGGGGGKRRGKKEDSHWWSRFQKGDFPWDDKDFRMYFLWTALFWGGVMIYFVFKSSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDGSFLLSMLPTVLIIAFLLYTIRRGPAGIGRTGRGMGGLFSVGETTAKVLKDEIDVKFKDVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAILTGPPGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRGNFGGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSALEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDAINEKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARMIDDEVRILISDAYRRTVALLTEKKADVEKVALLLLEKEVLDKNDMVQLLGPRPFTEKSTYEEFVEGTGSLDEDTSLPEGLQDWNKEREKEEKKEKEKEEPLNEKVVS

Target ORF information:

RefSeq Version	NM_027130.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027130.2

ORF Insert Sequence:

ATGGCCCACC GCTGCCTGCT GCTGTGGAGC CGGGGCGGCT GCCGTCGCGG CCTTCCTCCC 
CTGCTCGTGC CCAGAGGTTG CCTGGGTCCG GACCGGCGGC CCTGCCTCCG TACGCTCTAT 
CAATATGCTA CTGTCCAGAC AGCAAGCAGC AGGCGTTCTC TGCTGAGGGA TGTAATTGCT 
GCTTATCAAA GATTCTGTTC TCGACCTCCC AAAGGATTTG AAAAGTACTT TCCTAATGGG 
AAAAACGGAA AAAAGGCCAG TGAGCCTAAG GAGGCTGTTG GAGAAAAAAA AGAACCACAG 
CCCTCGGGCC CCCAGCCTTC TGGAGGTGCA GGTGGTGGGG GAGGGAAGCG CCGTGGCAAG 
AAAGAAGATT CTCACTGGTG GTCCAGGTTC CAGAAGGGTG ACTTCCCATG GGATGACAAG 
GATTTCAGGA TGTACTTTCT CTGGACTGCT CTTTTTTGGG GTGGAGTCAT GATTTACTTC 
GTGTTCAAGA GCTCTGGGAG AGAAATCACG TGGAAAGACT TTGTCAATAA CTATCTTTCT 
AAGGGCGTGG TGGACAGACT AGAAGTTGTC AACAAGCGTT TTGTTCGTGT GACCTTTACA 
CCAGGAAAAA CTCCGGTTGA TGGGCAATAC GTCTGGTTTA ATATTGGCAG TGTTGACACA 
TTTGAGCGGA ATCTGGAGAC TTTGCAGCAA GAATTGGGCA TAGAAGGGGA GAACCGGGTC 
CCTGTGGTTT ATATTGCTGA GAGCGATGGC TCCTTCCTGC TGAGCATGTT GCCCACCGTA 
CTCATTATCG CTTTTCTACT CTACACCATA AGAAGAGGGC CCGCTGGCAT TGGTCGGACC 
GGCCGGGGAA TGGGTGGACT CTTCAGCGTT GGGGAAACCA CAGCCAAGGT CTTAAAGGAT 
GAGATAGATG TGAAGTTTAA AGATGTGGCT GGCTGTGAGG AGGCCAAGCT AGAAATAATG 
GAATTCGTGA ATTTCTTGAA AAACCCAAAG CAATATCAAG ACCTAGGAGC AAAAATCCCA 
AAGGGTGCCA TTCTCACCGG TCCCCCAGGT ACTGGGAAGA CGCTGCTAGC TAAGGCCACA 
GCTGGAGAAG CCAATGTCCC CTTTATCACT GTGAGCGGAT CTGAGTTTCT GGAGATGTTT 
GTTGGCGTTG GTCCAGCCAG AGTCCGAGAC TTATTTGCCC TTGCTCGGAA GAATGCGCCT 
TGCATTCTCT TCATTGATGA GATTGATGCT GTGGGAAGGA AGCGCGGCAG AGGCAACTTC 
GGTGGGCAGA GCGAGCAGGA GAACACACTC AACCAGCTGC TTGTGGAGAT GGACGGCTTC 
AACACAACCA CCAATGTGGT CATCCTGGCA GGCACAAATC GACCAGACAT CCTGGATCCA 
GCTCTGTTGA GACCAGGCCG CTTTGACAGG CAGATTTTTA TTGGACCCCC AGACATAAAA 
GGACGAGCCT CAATCTTCAA AGTTCACCTT CGACCATTGA AGCTGGACAG TGCCTTGGAA 
AAAGATAAAT TGGCCAGAAA ACTGGCGTCC TTAACTCCAG GGTTTTCAGG CGCTGATGTT 
GCCAATGTCT GCAATGAAGC TGCTTTGATT GCTGCAAGAC ACCTTTCAGA TGCCATTAAT 
GAGAAGCACT TCGAACAAGC GATTGAGCGA GTGATTGGAG GCTTGGAGAA AAAAACCCAA 
GTTCTGCAGC CTGAGGAGAA GAAGACGGTG GCTTACCACG AAGCAGGCCA TGCGGTCGCT 
GGCTGGTATC TGGAGCATGC AGACCCACTC TTAAAGGTCT CCATCATCCC GCGTGGCAAG 
GGGCTGGGCT ATGCTCAGTA CTTGCCCAAG GAGCAGTATC TGTACACAAA GGAGCAGCTG 
CTGGACAGGA TGTGCATGAC TCTGGGCGGC CGTGTCTCCG AGGAGATCTT CTTTGGGAGA 
ATTACAACCG GTGCCCAGGA CGACTTGAGG AAGGTTACCC AGAGTGCCTA TGCCCAGATC 
GTTCAGTTTG GCATGAACGA GAAAGTGGGG CAGATCTCCT TTGACCTCCC ACGACAGGGG 
GACATGGTGT TAGAGAAGCC TTACAGTGAG GCCACTGCGA GGATGATAGA CGATGAAGTG 
AGGATACTCA TCAGCGATGC CTACAGAAGG ACGGTGGCTC TTCTCACAGA GAAGAAGGCT 
GACGTGGAGA AGGTCGCTCT CTTACTGTTA GAAAAGGAAG TCCTAGACAA GAATGACATG 
GTCCAGCTTC TCGGTCCCAG ACCATTTACA GAAAAGTCCA CATATGAAGA ATTTGTGGAA 
GGCACTGGCA GCTTAGACGA GGACACTTCT CTTCCTGAAG GCCTTCAGGA TTGGAACAAG 
GAGCGGGAGA AGGAGGAGAA GAAGGAGAAG GAGAAGGAGG AGCCGCTGAA TGAGAAGGTT 
GTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afg3l2 ( NM_027130.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081406.1 Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), mRNA.

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CloneID	OMu23383
Clone ID Related Accession (Same CDS sequence)	NM_027130.2 , NM_027130.1
Accession Version	NM_027130.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	AFG3-like protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC043056.1. On or before Jul 24, 2006 this sequence version replaced XM_128950.7, XM_001000481.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043056.1, SRR1660823.91364.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCCCACC GCTGCCTGCT GCTGTGGAGC CGGGGCGGCT GCCGTCGCGG CCTTCCTCCC 
CTGCTCGTGC CCAGAGGTTG CCTGGGTCCG GACCGGCGGC CCTGCCTCCG TACGCTCTAT 
CAATATGCTA CTGTCCAGAC AGCAAGCAGC AGGCGTTCTC TGCTGAGGGA TGTAATTGCT 
GCTTATCAAA GATTCTGTTC TCGACCTCCC AAAGGATTTG AAAAGTACTT TCCTAATGGG 
AAAAACGGAA AAAAGGCCAG TGAGCCTAAG GAGGCTGTTG GAGAAAAAAA AGAACCACAG 
CCCTCGGGCC CCCAGCCTTC TGGAGGTGCA GGTGGTGGGG GAGGGAAGCG CCGTGGCAAG 
AAAGAAGATT CTCACTGGTG GTCCAGGTTC CAGAAGGGTG ACTTCCCATG GGATGACAAG 
GATTTCAGGA TGTACTTTCT CTGGACTGCT CTTTTTTGGG GTGGAGTCAT GATTTACTTC 
GTGTTCAAGA GCTCTGGGAG AGAAATCACG TGGAAAGACT TTGTCAATAA CTATCTTTCT 
AAGGGCGTGG TGGACAGACT AGAAGTTGTC AACAAGCGTT TTGTTCGTGT GACCTTTACA 
CCAGGAAAAA CTCCGGTTGA TGGGCAATAC GTCTGGTTTA ATATTGGCAG TGTTGACACA 
TTTGAGCGGA ATCTGGAGAC TTTGCAGCAA GAATTGGGCA TAGAAGGGGA GAACCGGGTC 
CCTGTGGTTT ATATTGCTGA GAGCGATGGC TCCTTCCTGC TGAGCATGTT GCCCACCGTA 
CTCATTATCG CTTTTCTACT CTACACCATA AGAAGAGGGC CCGCTGGCAT TGGTCGGACC 
GGCCGGGGAA TGGGTGGACT CTTCAGCGTT GGGGAAACCA CAGCCAAGGT CTTAAAGGAT 
GAGATAGATG TGAAGTTTAA AGATGTGGCT GGCTGTGAGG AGGCCAAGCT AGAAATAATG 
GAATTCGTGA ATTTCTTGAA AAACCCAAAG CAATATCAAG ACCTAGGAGC AAAAATCCCA 
AAGGGTGCCA TTCTCACCGG TCCCCCAGGT ACTGGGAAGA CGCTGCTAGC TAAGGCCACA 
GCTGGAGAAG CCAATGTCCC CTTTATCACT GTGAGCGGAT CTGAGTTTCT GGAGATGTTT 
GTTGGCGTTG GTCCAGCCAG AGTCCGAGAC TTATTTGCCC TTGCTCGGAA GAATGCGCCT 
TGCATTCTCT TCATTGATGA GATTGATGCT GTGGGAAGGA AGCGCGGCAG AGGCAACTTC 
GGTGGGCAGA GCGAGCAGGA GAACACACTC AACCAGCTGC TTGTGGAGAT GGACGGCTTC 
AACACAACCA CCAATGTGGT CATCCTGGCA GGCACAAATC GACCAGACAT CCTGGATCCA 
GCTCTGTTGA GACCAGGCCG CTTTGACAGG CAGATTTTTA TTGGACCCCC AGACATAAAA 
GGACGAGCCT CAATCTTCAA AGTTCACCTT CGACCATTGA AGCTGGACAG TGCCTTGGAA 
AAAGATAAAT TGGCCAGAAA ACTGGCGTCC TTAACTCCAG GGTTTTCAGG CGCTGATGTT 
GCCAATGTCT GCAATGAAGC TGCTTTGATT GCTGCAAGAC ACCTTTCAGA TGCCATTAAT 
GAGAAGCACT TCGAACAAGC GATTGAGCGA GTGATTGGAG GCTTGGAGAA AAAAACCCAA 
GTTCTGCAGC CTGAGGAGAA GAAGACGGTG GCTTACCACG AAGCAGGCCA TGCGGTCGCT 
GGCTGGTATC TGGAGCATGC AGACCCACTC TTAAAGGTCT CCATCATCCC GCGTGGCAAG 
GGGCTGGGCT ATGCTCAGTA CTTGCCCAAG GAGCAGTATC TGTACACAAA GGAGCAGCTG 
CTGGACAGGA TGTGCATGAC TCTGGGCGGC CGTGTCTCCG AGGAGATCTT CTTTGGGAGA 
ATTACAACCG GTGCCCAGGA CGACTTGAGG AAGGTTACCC AGAGTGCCTA TGCCCAGATC 
GTTCAGTTTG GCATGAACGA GAAAGTGGGG CAGATCTCCT TTGACCTCCC ACGACAGGGG 
GACATGGTGT TAGAGAAGCC TTACAGTGAG GCCACTGCGA GGATGATAGA CGATGAAGTG 
AGGATACTCA TCAGCGATGC CTACAGAAGG ACGGTGGCTC TTCTCACAGA GAAGAAGGCT 
GACGTGGAGA AGGTCGCTCT CTTACTGTTA GAAAAGGAAG TCCTAGACAA GAATGACATG 
GTCCAGCTTC TCGGTCCCAG ACCATTTACA GAAAAGTCCA CATATGAAGA ATTTGTGGAA 
GGCACTGGCA GCTTAGACGA GGACACTTCT CTTCCTGAAG GCCTTCAGGA TTGGAACAAG 
GAGCGGGAGA AGGAGGAGAA GAAGGAGAAG GAGAAGGAGG AGCCGCTGAA TGAGAAGGTT 
GTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081406.1
CDS	136..2544
Translation	MAHRCLLLWSRGGCRRGLPPLLVPRGCLGPDRRPCLRTLYQYATVQTASSRRSLLRDVIAAYQRFCSRPPKGFEKYFPNGKNGKKASEPKEAVGEKKEPQPSGPQPSGGAGGGGGKRRGKKEDSHWWSRFQKGDFPWDDKDFRMYFLWTALFWGGVMIYFVFKSSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDGSFLLSMLPTVLIIAFLLYTIRRGPAGIGRTGRGMGGLFSVGETTAKVLKDEIDVKFKDVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAILTGPPGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRGNFGGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSALEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDAINEKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARMIDDEVRILISDAYRRTVALLTEKKADVEKVALLLLEKEVLDKNDMVQLLGPRPFTEKSTYEEFVEGTGSLDEDTSLPEGLQDWNKEREKEEKKEKEKEEPLNEKVVS

Target ORF information:

RefSeq Version	NM_027130.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027130.1

ORF Insert Sequence:

ATGGCCCACC GCTGCCTGCT GCTGTGGAGC CGGGGCGGCT GCCGTCGCGG CCTTCCTCCC 
CTGCTCGTGC CCAGAGGTTG CCTGGGTCCG GACCGGCGGC CCTGCCTCCG TACGCTCTAT 
CAATATGCTA CTGTCCAGAC AGCAAGCAGC AGGCGTTCTC TGCTGAGGGA TGTAATTGCT 
GCTTATCAAA GATTCTGTTC TCGACCTCCC AAAGGATTTG AAAAGTACTT TCCTAATGGG 
AAAAACGGAA AAAAGGCCAG TGAGCCTAAG GAGGCTGTTG GAGAAAAAAA AGAACCACAG 
CCCTCGGGCC CCCAGCCTTC TGGAGGTGCA GGTGGTGGGG GAGGGAAGCG CCGTGGCAAG 
AAAGAAGATT CTCACTGGTG GTCCAGGTTC CAGAAGGGTG ACTTCCCATG GGATGACAAG 
GATTTCAGGA TGTACTTTCT CTGGACTGCT CTTTTTTGGG GTGGAGTCAT GATTTACTTC 
GTGTTCAAGA GCTCTGGGAG AGAAATCACG TGGAAAGACT TTGTCAATAA CTATCTTTCT 
AAGGGCGTGG TGGACAGACT AGAAGTTGTC AACAAGCGTT TTGTTCGTGT GACCTTTACA 
CCAGGAAAAA CTCCGGTTGA TGGGCAATAC GTCTGGTTTA ATATTGGCAG TGTTGACACA 
TTTGAGCGGA ATCTGGAGAC TTTGCAGCAA GAATTGGGCA TAGAAGGGGA GAACCGGGTC 
CCTGTGGTTT ATATTGCTGA GAGCGATGGC TCCTTCCTGC TGAGCATGTT GCCCACCGTA 
CTCATTATCG CTTTTCTACT CTACACCATA AGAAGAGGGC CCGCTGGCAT TGGTCGGACC 
GGCCGGGGAA TGGGTGGACT CTTCAGCGTT GGGGAAACCA CAGCCAAGGT CTTAAAGGAT 
GAGATAGATG TGAAGTTTAA AGATGTGGCT GGCTGTGAGG AGGCCAAGCT AGAAATAATG 
GAATTCGTGA ATTTCTTGAA AAACCCAAAG CAATATCAAG ACCTAGGAGC AAAAATCCCA 
AAGGGTGCCA TTCTCACCGG TCCCCCAGGT ACTGGGAAGA CGCTGCTAGC TAAGGCCACA 
GCTGGAGAAG CCAATGTCCC CTTTATCACT GTGAGCGGAT CTGAGTTTCT GGAGATGTTT 
GTTGGCGTTG GTCCAGCCAG AGTCCGAGAC TTATTTGCCC TTGCTCGGAA GAATGCGCCT 
TGCATTCTCT TCATTGATGA GATTGATGCT GTGGGAAGGA AGCGCGGCAG AGGCAACTTC 
GGTGGGCAGA GCGAGCAGGA GAACACACTC AACCAGCTGC TTGTGGAGAT GGACGGCTTC 
AACACAACCA CCAATGTGGT CATCCTGGCA GGCACAAATC GACCAGACAT CCTGGATCCA 
GCTCTGTTGA GACCAGGCCG CTTTGACAGG CAGATTTTTA TTGGACCCCC AGACATAAAA 
GGACGAGCCT CAATCTTCAA AGTTCACCTT CGACCATTGA AGCTGGACAG TGCCTTGGAA 
AAAGATAAAT TGGCCAGAAA ACTGGCGTCC TTAACTCCAG GGTTTTCAGG CGCTGATGTT 
GCCAATGTCT GCAATGAAGC TGCTTTGATT GCTGCAAGAC ACCTTTCAGA TGCCATTAAT 
GAGAAGCACT TCGAACAAGC GATTGAGCGA GTGATTGGAG GCTTGGAGAA AAAAACCCAA 
GTTCTGCAGC CTGAGGAGAA GAAGACGGTG GCTTACCACG AAGCAGGCCA TGCGGTCGCT 
GGCTGGTATC TGGAGCATGC AGACCCACTC TTAAAGGTCT CCATCATCCC GCGTGGCAAG 
GGGCTGGGCT ATGCTCAGTA CTTGCCCAAG GAGCAGTATC TGTACACAAA GGAGCAGCTG 
CTGGACAGGA TGTGCATGAC TCTGGGCGGC CGTGTCTCCG AGGAGATCTT CTTTGGGAGA 
ATTACAACCG GTGCCCAGGA CGACTTGAGG AAGGTTACCC AGAGTGCCTA TGCCCAGATC 
GTTCAGTTTG GCATGAACGA GAAAGTGGGG CAGATCTCCT TTGACCTCCC ACGACAGGGG 
GACATGGTGT TAGAGAAGCC TTACAGTGAG GCCACTGCGA GGATGATAGA CGATGAAGTG 
AGGATACTCA TCAGCGATGC CTACAGAAGG ACGGTGGCTC TTCTCACAGA GAAGAAGGCT 
GACGTGGAGA AGGTCGCTCT CTTACTGTTA GAAAAGGAAG TCCTAGACAA GAATGACATG 
GTCCAGCTTC TCGGTCCCAG ACCATTTACA GAAAAGTCCA CATATGAAGA ATTTGTGGAA 
GGCACTGGCA GCTTAGACGA GGACACTTCT CTTCCTGAAG GCCTTCAGGA TTGGAACAAG 
GAGCGGGAGA AGGAGGAGAA GAAGGAGAAG GAGAAGGAGG AGCCGCTGAA TGAGAAGGTT 
GTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afg3l2 ( XM_011246998.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245300.1 Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), transcript variant X1, mRNA.

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CloneID	OMu70436
Clone ID Related Accession (Same CDS sequence)	XM_011246998.2 , XM_011246998.1
Accession Version	XM_011246998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AFG3-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA CGGAAAAAAG GCCAGTGAGC CTAAGGAGGC TGTTGGAGAA AAAAAAAGAA 
CCACAGCCCT CGGGCCCCCA GCCTTCTGGA GGTGCAGGTG GTGGGGGAGG GAAGCGCCGT 
GGCAAGAAAG AAGATTCTCA CTGGTGGTCC AGGTTCCAGA AGGGTGACTT CCCATGGGAT 
GACAAGGATT TCAGGATGTA CTTTCTCTGG ACTGCTCTTT TTTGGGGTGG AGTCATGATT 
TACTTCGTGT TCAAGAGCTC TGGGAGAGAA ATCACGTGGA AAGACTTTGT CAATAACTAT 
CTTTCTAAGG GCGTGGTGGA CAGACTAGAA GTTGTCAACA AGCGTTTTGT TCGTGTGACC 
TTTACACCAG GAAAAACTCC GGTTGATGGG CAATACGTCT GGTTTAATAT TGGCAGTGTT 
GACACATTTG AGCGGAATCT GGAGACTTTG CAGCAAGAAT TGGGCATAGA AGGGGAGAAC 
CGGGTCCCTG TGGTTTATAT TGCTGAGAGC GATGGCTCCT TCCTGCTGAG CATGTTGCCC 
ACCGTACTCA TTATCGCTTT TCTACTCTAC ACCATAAGAA GAGGGCCCGC TGGCATTGGT 
CGGACCGGCC GGGGAATGGG TGGACTCTTC AGCGTTGGGG AAACCACAGC CAAGGTCTTA 
AAGGATGAGA TAGATGTGAA GTTTAAAGAT GTGGCTGGCT GTGAGGAGGC CAAGCTAGAA 
ATAATGGAAT TCGTGAATTT CTTGAAAAAC CCAAAGCAAT ATCAAGACCT AGGAGCAAAA 
ATCCCAAAGG GTGCCATTCT CACCGGTCCC CCAGGTACTG GGAAGACGCT GCTAGCTAAG 
GCCACAGCTG GAGAAGCCAA TGTCCCCTTT ATCACTGTGA GCGGATCTGA GTTTCTGGAG 
ATGTTTGTTG GCGTTGGTCC AGCCAGAGTC CGAGACTTAT TTGCCCTTGC TCGGAAGAAT 
GCGCCTTGCA TTCTCTTCAT TGATGAGATT GATGCTGTGG GAAGGAAGCG CGGCAGAGGC 
AACTTCGGTG GGCAGAGCGA GCAGGAGAAC ACACTCAACC AGCTGCTTGT GGAGATGGAC 
GGCTTCAACA CAACCACCAA TGTGGTCATC CTGGCAGGCA CAAATCGACC AGACATCCTG 
GATCCAGCTC TGTTGAGACC AGGCCGCTTT GACAGGCAGA TTTTTATTGG ACCCCCAGAC 
ATAAAAGGAC GAGCCTCAAT CTTCAAAGTT CACCTTCGAC CATTGAAGCT GGACAGTGCC 
TTGGAAAAAG ATAAATTGGC CAGAAAACTG GCGTCCTTAA CTCCAGGGTT TTCAGGCGCT 
GATGTTGCCA ATGTCTGCAA TGAAGCTGCT TTGATTGCTG CAAGACACCT TTCAGATGCC 
ATTAATGAGA AGCACTTCGA ACAAGCGATT GAGCGAGTGA TTGGAGGCTT GGAGAAAAAA 
ACCCAAGTTC TGCAGCCTGA GGAGAAGAAG ACGGTGGCTT ACCACGAAGC AGGCCATGCG 
GTCGCTGGCT GGTATCTGGA GCATGCAGAC CCACTCTTAA AGGTCTCCAT CATCCCGCGT 
GGCAAGGGGC TGGGCTATGC TCAGTACTTG CCCAAGGAGC AGTATCTGTA CACAAAGGAG 
CAGCTGCTGG ACAGGATGTG CATGACTCTG GGCGGCCGTG TCTCCGAGGA GATCTTCTTT 
GGGAGAATTA CAACCGGTGC CCAGGACGAC TTGAGGAAGG TTACCCAGAG TGCCTATGCC 
CAGATCGTTC AGTTTGGCAT GAACGAGAAA GTGGGGCAGA TCTCCTTTGA CCTCCCACGA 
CAGGGGGACA TGGTGTTAGA GAAGCCTTAC AGTGAGGCCA CTGCGAGGAT GATAGACGAT 
GAAGTGAGGA TACTCATCAG CGATGCCTAC AGAAGGACGG TGGCTCTTCT CACAGAGAAG 
AAGGCTGACG TGGAGAAGGT CGCTCTCTTA CTGTTAGAAA AGGAAGTCCT AGACAAGAAT 
GACATGGTCC AGCTTCTCGG TCCCAGACCA TTTACAGAAA AGTCCACATA TGAAGAATTT 
GTGGAAGGCA CTGGCAGCTT AGACGAGGAC ACTTCTCTTC CTGAAGGCCT TCAGGATTGG 
AACAAGGAGC GGGAGAAGGA GGAGAAGAAG GAGAAGGAGA AGGAGGAGCC GCTGAATGAG 
AAGGTTGTCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245300.1
CDS	1438..3612
Translation	MGKTEKRPVSLRRLLEKKKEPQPSGPQPSGGAGGGGGKRRGKKEDSHWWSRFQKGDFPWDDKDFRMYFLWTALFWGGVMIYFVFKSSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDGSFLLSMLPTVLIIAFLLYTIRRGPAGIGRTGRGMGGLFSVGETTAKVLKDEIDVKFKDVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAILTGPPGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRGNFGGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSALEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDAINEKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARMIDDEVRILISDAYRRTVALLTEKKADVEKVALLLLEKEVLDKNDMVQLLGPRPFTEKSTYEEFVEGTGSLDEDTSLPEGLQDWNKEREKEEKKEKEKEEPLNEKVVS

Target ORF information:

RefSeq Version	XM_011246998.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246998.2

ORF Insert Sequence:

ATGGGAAAAA CGGAAAAAAG GCCAGTGAGC CTAAGGAGGC TGTTGGAGAA AAAAAAAGAA 
CCACAGCCCT CGGGCCCCCA GCCTTCTGGA GGTGCAGGTG GTGGGGGAGG GAAGCGCCGT 
GGCAAGAAAG AAGATTCTCA CTGGTGGTCC AGGTTCCAGA AGGGTGACTT CCCATGGGAT 
GACAAGGATT TCAGGATGTA CTTTCTCTGG ACTGCTCTTT TTTGGGGTGG AGTCATGATT 
TACTTCGTGT TCAAGAGCTC TGGGAGAGAA ATCACGTGGA AAGACTTTGT CAATAACTAT 
CTTTCTAAGG GCGTGGTGGA CAGACTAGAA GTTGTCAACA AGCGTTTTGT TCGTGTGACC 
TTTACACCAG GAAAAACTCC GGTTGATGGG CAATACGTCT GGTTTAATAT TGGCAGTGTT 
GACACATTTG AGCGGAATCT GGAGACTTTG CAGCAAGAAT TGGGCATAGA AGGGGAGAAC 
CGGGTCCCTG TGGTTTATAT TGCTGAGAGC GATGGCTCCT TCCTGCTGAG CATGTTGCCC 
ACCGTACTCA TTATCGCTTT TCTACTCTAC ACCATAAGAA GAGGGCCCGC TGGCATTGGT 
CGGACCGGCC GGGGAATGGG TGGACTCTTC AGCGTTGGGG AAACCACAGC CAAGGTCTTA 
AAGGATGAGA TAGATGTGAA GTTTAAAGAT GTGGCTGGCT GTGAGGAGGC CAAGCTAGAA 
ATAATGGAAT TCGTGAATTT CTTGAAAAAC CCAAAGCAAT ATCAAGACCT AGGAGCAAAA 
ATCCCAAAGG GTGCCATTCT CACCGGTCCC CCAGGTACTG GGAAGACGCT GCTAGCTAAG 
GCCACAGCTG GAGAAGCCAA TGTCCCCTTT ATCACTGTGA GCGGATCTGA GTTTCTGGAG 
ATGTTTGTTG GCGTTGGTCC AGCCAGAGTC CGAGACTTAT TTGCCCTTGC TCGGAAGAAT 
GCGCCTTGCA TTCTCTTCAT TGATGAGATT GATGCTGTGG GAAGGAAGCG CGGCAGAGGC 
AACTTCGGTG GGCAGAGCGA GCAGGAGAAC ACACTCAACC AGCTGCTTGT GGAGATGGAC 
GGCTTCAACA CAACCACCAA TGTGGTCATC CTGGCAGGCA CAAATCGACC AGACATCCTG 
GATCCAGCTC TGTTGAGACC AGGCCGCTTT GACAGGCAGA TTTTTATTGG ACCCCCAGAC 
ATAAAAGGAC GAGCCTCAAT CTTCAAAGTT CACCTTCGAC CATTGAAGCT GGACAGTGCC 
TTGGAAAAAG ATAAATTGGC CAGAAAACTG GCGTCCTTAA CTCCAGGGTT TTCAGGCGCT 
GATGTTGCCA ATGTCTGCAA TGAAGCTGCT TTGATTGCTG CAAGACACCT TTCAGATGCC 
ATTAATGAGA AGCACTTCGA ACAAGCGATT GAGCGAGTGA TTGGAGGCTT GGAGAAAAAA 
ACCCAAGTTC TGCAGCCTGA GGAGAAGAAG ACGGTGGCTT ACCACGAAGC AGGCCATGCG 
GTCGCTGGCT GGTATCTGGA GCATGCAGAC CCACTCTTAA AGGTCTCCAT CATCCCGCGT 
GGCAAGGGGC TGGGCTATGC TCAGTACTTG CCCAAGGAGC AGTATCTGTA CACAAAGGAG 
CAGCTGCTGG ACAGGATGTG CATGACTCTG GGCGGCCGTG TCTCCGAGGA GATCTTCTTT 
GGGAGAATTA CAACCGGTGC CCAGGACGAC TTGAGGAAGG TTACCCAGAG TGCCTATGCC 
CAGATCGTTC AGTTTGGCAT GAACGAGAAA GTGGGGCAGA TCTCCTTTGA CCTCCCACGA 
CAGGGGGACA TGGTGTTAGA GAAGCCTTAC AGTGAGGCCA CTGCGAGGAT GATAGACGAT 
GAAGTGAGGA TACTCATCAG CGATGCCTAC AGAAGGACGG TGGCTCTTCT CACAGAGAAG 
AAGGCTGACG TGGAGAAGGT CGCTCTCTTA CTGTTAGAAA AGGAAGTCCT AGACAAGAAT 
GACATGGTCC AGCTTCTCGG TCCCAGACCA TTTACAGAAA AGTCCACATA TGAAGAATTT 
GTGGAAGGCA CTGGCAGCTT AGACGAGGAC ACTTCTCTTC CTGAAGGCCT TCAGGATTGG 
AACAAGGAGC GGGAGAAGGA GGAGAAGAAG GAGAAGGAGA AGGAGGAGCC GCTGAATGAG 
AAGGTTGTCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Mitochondrial m-AAA Protease Prevents Demyelination and Hair Greying.
PLoS genetics12(12)e1006463(2016 Dec)
Wang S,Jacquemyn J,Murru S,Martinelli P,Barth E,Langer T,Niessen CM,Rugarli EI

The m-AAA Protease Associated with Neurodegeneration Limits MCU Activity in Mitochondria.
Molecular cell64(1)148-162(2016 Oct)
K?nig T,Tr?der SE,Bakka K,Korwitz A,Richter-Dennerlein R,Lampe PA,Patron M,M?hlmeister M,Guerrero-Castillo S,Brandt U,Decker T,Lauria I,Paggio A,Rizzuto R,Rugarli EI,De Stefani D,Langer T

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS genetics12(9)e1006355(2016 Sep)
Wu Y,Wei FY,Kawarada L,Suzuki T,Araki K,Komohara Y,Fujimura A,Kaitsuka T,Takeya M,Oike Y,Suzuki T,Tomizawa K

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.
The Journal of cell biology211(2)373-89(2015 Oct)
Richter U,Lahtinen T,Marttinen P,Suomi F,Battersby BJ

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Afg3l2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

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