Pcgf3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcgf3
Entrez Gene ID	69587
Full Name	polycomb group ring finger 3
Synonyms	2310035N15Rik,AI662857,D630042K08Rik,DONG1,E430039C14,RNF3A,Rnf3
General protein information	Preferred Names polycomb group ring finger 3 Names polycomb group RING finger protein 3 RING finger protein 3A ring finger protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_172716.4	NP_766304.2	polycomb group RING finger protein 3
NM_172716.5	NP_766304.2	polycomb group RING finger protein 3 isoform 1
NM_001377001.1	NP_001363930.1	polycomb group RING finger protein 3 isoform 1
XM_006535208.2	XP_006535271.1	polycomb group RING finger protein 3 isoform X1
XM_030254794.1	XP_030110654.1	polycomb group RING finger protein 3 isoform X2
NM_001377000.1	NP_001363929.1	polycomb group RING finger protein 3 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PCGF3	XP_003339483.1
	PCGF3	XP_002802324.1
Rattus norvegicus (Norway rat)	Pcgf3	NP_001100715.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002533	XP_312405.2
Canis lupus familiaris (dog)	PCGF3	XP_005618769.1
Drosophila melanogaster (fruit fly)	l(3)73Ah	NP_524933.1
Xenopus tropicalis (tropical clawed frog)	pcgf3	NP_001016847.1
Homo sapiens (human)	PCGF3	NP_006306.2
Gallus gallus (chicken)	PCGF3	XP_004949372.1
Caenorhabditis elegans (roundworm)	mig-32	NP_001255620.1
Bos taurus (cattle)	PCGF3	NP_001040032.1
Mus musculus (house mouse)	Pcgf3	NP_766304.2

Gene Ontology
Bibliography

Related articles in PubMed

Polycomb group RING finger proteins 3/5 activate transcription via an interaction with the pluripotency factor Tex10 in embryonic stem cells.
Zhao W, Huang Y, Zhang J, Liu M, Ji H, Wang C, Cao N, Li C, Xia Y, Jiang Q, Qin J
The Journal of biological chemistry292(52)21527-21537(2017 Dec)

hnRNPK Recruits PCGF3/5-PRC1 to the Xist RNA B-Repeat to Establish Polycomb-Mediated Chromosomal Silencing.
Pintacuda G, Wei G, Roustan C, Kirmizitas BA, Solcan N, Cerase A, Castello A, Mohammed S, Moindrot B, Nesterova TB, Brockdorff N
Molecular cell68(5)955-969.e10(2017 Dec)

PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.
Almeida M, Pintacuda G, Masui O, Koseki Y, Gdula M, Cerase A, Brown D, Mould A, Innocent C, Nakayama M, Schermelleh L, Nesterova TB, Koseki H, Brockdorff N
Science (New York, N.Y.)356(6342)1081-1084(2017 06)

Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity.
Zhao W, Tong H, Huang Y, Yan Y, Teng H, Xia Y, Jiang Q, Qin J
The Journal of biological chemistry292(7)2773-2784(2017 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

hnRNPK recruits PCGF3/5-PRC1 to the Xist RNA B-repeat to establish polycomb-mediated chromosomal silencing.
Title: hnRNPK Recruits PCGF3/5-PRC1 to the Xist RNA B-Repeat to Establish Polycomb-Mediated Chromosomal Silencing.

The following Pcgf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcgf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu35662	XM_006535208.2 Latest version!	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu03944	NM_172716.5 Latest version!	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu03944	NM_001377001.1 Latest version!	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu35662	NM_001377000.1 Latest version!	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu03944	XM_030254794.1 Latest version!	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu03944	NM_172716.4	Mus musculus polycomb group ring finger 3 (Pcgf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcgf3 ( NM_172716.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766304.2 Mus musculus polycomb group ring finger 3 (Pcgf3), mRNA.

Price & Availability↑

CloneID	OMu03944
Clone ID Related Accession (Same CDS sequence)	NM_172716.4 , NM_172716.5 , NM_001377001.1 , XM_030254794.1
Accession Version	NM_172716.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	polycomb group RING finger protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC158912.3 and AC133896.7. On May 2, 2007 this sequence version replaced NM_172716.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK089091.1, SRR1660821.1637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAA GGCACCCCTC CTGCTACACT ACAGACCCAA GATGGACTTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766304.2
CDS	311..1036
Translation	MLTRKIKLWDINAHITCRLCSGYLIDATTVTECLHTFCRSCLVKYLEENNTCPTCRIVIHQSHPLQYIGHDRTMQDIVYKLVPGLQEAEMRKQREFYHKLGMEVPGDIKGEACSAKQHLDPRNGETKADDNSNKETAEEKQEEDNDYHRSDEQVSICLECNSSKLRGLKRKWIRCSAQATVLHLKKFIAKKLNLSSFNELDILCNEEILGKDHTLKFVVVTRWRFKKAPLLLHYRPKMDLL

Target ORF information:

RefSeq Version	NM_172716.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus polycomb group ring finger 3 (Pcgf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172716.4

ORF Insert Sequence:

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAA GGCACCCCTC CTGCTACACT ACAGACCCAA GATGGACTTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf3 ( XM_006535208.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535271.1 Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35662
Clone ID Related Accession (Same CDS sequence)	XM_006535208.2 , NM_001377000.1
Accession Version	XM_006535208.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	polycomb group RING finger protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006535208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAC ATTGCTGTGG AACAGCTGTC TTTCTGGATC CACCCACCCA 
GCCAGGGTCT TTATCACCAC TCCACTCGAT CCCCTGAATC CAGGGGATCA GATGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535271.1
CDS	428..1207
Translation	MLTRKIKLWDINAHITCRLCSGYLIDATTVTECLHTFCRSCLVKYLEENNTCPTCRIVIHQSHPLQYIGHDRTMQDIVYKLVPGLQEAEMRKQREFYHKLGMEVPGDIKGEACSAKQHLDPRNGETKADDNSNKETAEEKQEEDNDYHRSDEQVSICLECNSSKLRGLKRKWIRCSAQATVLHLKKFIAKKLNLSSFNELDILCNEEILGKDHTLKFVVVTRWRFKTLLWNSCLSGSTHPARVFITTPLDPLNPGDQMW

Target ORF information:

RefSeq Version	XM_006535208.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535208.2

ORF Insert Sequence:

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAC ATTGCTGTGG AACAGCTGTC TTTCTGGATC CACCCACCCA 
GCCAGGGTCT TTATCACCAC TCCACTCGAT CCCCTGAATC CAGGGGATCA GATGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf3 ( NM_172716.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766304.2 Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03944
Clone ID Related Accession (Same CDS sequence)	NM_172716.4 , NM_172716.5 , NM_001377001.1 , XM_030254794.1
Accession Version	NM_172716.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Mus musculus(house mouse)
Product	polycomb group RING finger protein 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158912.3 and AC133896.7. On Dec 6, 2019 this sequence version replaced NM_172716.4. ##Evidence-Data-START## Transcript exon combination :: AK089091.1, AK085574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAA GGCACCCCTC CTGCTACACT ACAGACCCAA GATGGACTTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766304.2
CDS	384..1109
Translation	MLTRKIKLWDINAHITCRLCSGYLIDATTVTECLHTFCRSCLVKYLEENNTCPTCRIVIHQSHPLQYIGHDRTMQDIVYKLVPGLQEAEMRKQREFYHKLGMEVPGDIKGEACSAKQHLDPRNGETKADDNSNKETAEEKQEEDNDYHRSDEQVSICLECNSSKLRGLKRKWIRCSAQATVLHLKKFIAKKLNLSSFNELDILCNEEILGKDHTLKFVVVTRWRFKKAPLLLHYRPKMDLL

Target ORF information:

RefSeq Version	NM_172716.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172716.5

ORF Insert Sequence:

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAA GGCACCCCTC CTGCTACACT ACAGACCCAA GATGGACTTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf3 ( NM_001377001.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001363930.1 Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu03944
Clone ID Related Accession (Same CDS sequence)	NM_172716.4 , NM_172716.5 , NM_001377001.1 , XM_030254794.1
Accession Version	NM_001377001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Mus musculus(house mouse)
Product	polycomb group RING finger protein 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158912.3 and AC133896.7. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2100386.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAA GGCACCCCTC CTGCTACACT ACAGACCCAA GATGGACTTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363930.1
CDS	588..1313
Translation	MLTRKIKLWDINAHITCRLCSGYLIDATTVTECLHTFCRSCLVKYLEENNTCPTCRIVIHQSHPLQYIGHDRTMQDIVYKLVPGLQEAEMRKQREFYHKLGMEVPGDIKGEACSAKQHLDPRNGETKADDNSNKETAEEKQEEDNDYHRSDEQVSICLECNSSKLRGLKRKWIRCSAQATVLHLKKFIAKKLNLSSFNELDILCNEEILGKDHTLKFVVVTRWRFKKAPLLLHYRPKMDLL

Target ORF information:

RefSeq Version	NM_001377001.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377001.1

ORF Insert Sequence:

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAA GGCACCCCTC CTGCTACACT ACAGACCCAA GATGGACTTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf3 ( NM_001377000.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001363929.1 Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 2, mRNA.

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CloneID	OMu35662
Clone ID Related Accession (Same CDS sequence)	XM_006535208.2 , NM_001377000.1
Accession Version	NM_001377000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Mus musculus(house mouse)
Product	polycomb group RING finger protein 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158912.3 and AC133896.7. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAC ATTGCTGTGG AACAGCTGTC TTTCTGGATC CACCCACCCA 
GCCAGGGTCT TTATCACCAC TCCACTCGAT CCCCTGAATC CAGGGGATCA GATGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363929.1
CDS	384..1163
Translation	MLTRKIKLWDINAHITCRLCSGYLIDATTVTECLHTFCRSCLVKYLEENNTCPTCRIVIHQSHPLQYIGHDRTMQDIVYKLVPGLQEAEMRKQREFYHKLGMEVPGDIKGEACSAKQHLDPRNGETKADDNSNKETAEEKQEEDNDYHRSDEQVSICLECNSSKLRGLKRKWIRCSAQATVLHLKKFIAKKLNLSSFNELDILCNEEILGKDHTLKFVVVTRWRFKTLLWNSCLSGSTHPARVFITTPLDPLNPGDQMW

Target ORF information:

RefSeq Version	NM_001377000.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377000.1

ORF Insert Sequence:

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAC ATTGCTGTGG AACAGCTGTC TTTCTGGATC CACCCACCCA 
GCCAGGGTCT TTATCACCAC TCCACTCGAT CCCCTGAATC CAGGGGATCA GATGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf3 ( XM_030254794.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110654.1 Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu03944
Clone ID Related Accession (Same CDS sequence)	NM_172716.4 , NM_172716.5 , NM_001377001.1 , XM_030254794.1
Accession Version	XM_030254794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	polycomb group RING finger protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAA GGCACCCCTC CTGCTACACT ACAGACCCAA GATGGACTTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110654.1
CDS	1540..2265
Translation	MLTRKIKLWDINAHITCRLCSGYLIDATTVTECLHTFCRSCLVKYLEENNTCPTCRIVIHQSHPLQYIGHDRTMQDIVYKLVPGLQEAEMRKQREFYHKLGMEVPGDIKGEACSAKQHLDPRNGETKADDNSNKETAEEKQEEDNDYHRSDEQVSICLECNSSKLRGLKRKWIRCSAQATVLHLKKFIAKKLNLSSFNELDILCNEEILGKDHTLKFVVVTRWRFKKAPLLLHYRPKMDLL

Target ORF information:

RefSeq Version	XM_030254794.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polycomb group ring finger 3 (Pcgf3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254794.1

ORF Insert Sequence:

ATGTTGACCA GGAAGATTAA ACTCTGGGAT ATAAATGCCC ACATCACCTG CCGCCTGTGC 
AGCGGCTACC TCATTGACGC AACCACAGTG ACTGAGTGTT TGCACACATT CTGCAGGAGC 
TGCCTGGTGA AGTATCTGGA GGAGAATAAT ACCTGCCCCA CCTGCAGGAT CGTGATCCAT 
CAGAGCCACC CATTACAGTA TATCGGTCAT GACAGAACCA TGCAGGACAT TGTTTACAAG 
CTGGTGCCAG GCCTCCAGGA AGCGGAAATG AGGAAACAGA GGGAATTCTA TCACAAACTG 
GGCATGGAGG TACCAGGTGA CATCAAGGGG GAGGCGTGCT CAGCAAAACA GCACTTAGAT 
CCCCGAAATG GTGAAACCAA AGCAGACGAC AATTCAAATA AAGAGACAGC AGAGGAGAAG 
CAGGAGGAGG ACAATGACTA CCACAGGAGT GACGAGCAGG TAAGCATCTG TCTGGAATGC 
AACAGCAGCA AACTACGTGG CCTCAAGCGG AAGTGGATCA GATGCTCAGC CCAAGCAACT 
GTGCTGCACC TGAAAAAATT TATCGCCAAG AAGCTTAACC TCTCCTCCTT TAATGAGCTG 
GACATTTTAT GCAATGAGGA GATCCTGGGC AAGGACCACA CTCTCAAGTT CGTGGTTGTT 
ACAAGATGGA GATTCAAGAA GGCACCCCTC CTGCTACACT ACAGACCCAA GATGGACTTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polycomb group RING finger proteins 3/5 activate transcription via an interaction with the pluripotency factor Tex10 in embryonic stem cells.
The Journal of biological chemistry292(52)21527-21537(2017 Dec)
Zhao W,Huang Y,Zhang J,Liu M,Ji H,Wang C,Cao N,Li C,Xia Y,Jiang Q,Qin J

hnRNPK Recruits PCGF3/5-PRC1 to the Xist RNA B-Repeat to Establish Polycomb-Mediated Chromosomal Silencing.
Molecular cell68(5)955-969.e10(2017 Dec)
Pintacuda G,Wei G,Roustan C,Kirmizitas BA,Solcan N,Cerase A,Castello A,Mohammed S,Moindrot B,Nesterova TB,Brockdorff N

PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.
Science (New York, N.Y.)356(6342)1081-1084(2017 06)
Almeida M,Pintacuda G,Masui O,Koseki Y,Gdula M,Cerase A,Brown D,Mould A,Innocent C,Nakayama M,Schermelleh L,Nesterova TB,Koseki H,Brockdorff N

Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity.
The Journal of biological chemistry292(7)2773-2784(2017 02)
Zhao W,Tong H,Huang Y,Yan Y,Teng H,Xia Y,Jiang Q,Qin J

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Pcgf3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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