Med27 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med27
Entrez Gene ID	68975
Full Name	mediator complex subunit 27
Synonyms	1500015J03Rik,2310042P07Rik,AA682045,Crsp8,D2Ertd434e
General protein information	Preferred Names mediator complex subunit 27 Names mediator of RNA polymerase II transcription subunit 27 CRSP complex subunit 8 cofactor required for Sp1 transcriptional activation, subunit 8 transcriptional co-activator CRSP8 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 B\|2 19.84 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_026896.5	NP_081172.2	mediator of RNA polymerase II transcription subunit 27 isoform a
XM_017319247.2	XP_017174736.1	mediator of RNA polymerase II transcription subunit 27 isoform X2
XM_017319246.1	XP_017174735.1	mediator of RNA polymerase II transcription subunit 27 isoform X1
XM_017319247.1	XP_017174736.1	mediator of RNA polymerase II transcription subunit 27 isoform X2
NM_029615.1	NP_083891.1	mediator of RNA polymerase II transcription subunit 27 isoform b
NM_001290489.1	NP_001277418.1	mediator of RNA polymerase II transcription subunit 27 isoform c
NM_001290490.1	NP_001277419.1	mediator of RNA polymerase II transcription subunit 27 isoform d

Pathways from BioSystems

Homologies

	MED27	XP_001105126.1
Danio rerio (zebrafish)	med27	NP_956954.1
Mus musculus (house mouse)	Med27	NP_081172.2
Anopheles gambiae (African malaria mosquito)	MED27_ANOGA	XP_312260.3
Homo sapiens (human)	MED27	NP_004260.2
Rattus norvegicus (Norway rat)	Med27	NP_001100035.1
Gallus gallus (chicken)	MED27	NP_001264204.1
Drosophila melanogaster (fruit fly)	MED27	NP_649569.1
Bos taurus (cattle)	MED27	NP_001035636.1
Canis lupus familiaris (dog)	MED27	XP_849826.1
Xenopus tropicalis (tropical clawed frog)	med27	XP_004916727.1

Gene Ontology
Bibliography

Related articles in PubMed

Mediator and cohesin connect gene expression and chromatin architecture.
Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL, Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J, Young RA
Nature467(7314)430-5(2010 Sep)

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC, Tora L
EMBO reports11(7)541-7(2010 Jul)

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P, Eppig JJ, Solter D, Knowles BB
Genes & development20(19)2713-27(2006 Oct)

Cloning of wrinkle-free, a previously uncharacterized mouse mutation, reveals crucial roles for fatty acid transport protein 4 in skin and hair development.
Moulson CL, Martin DR, Lugus JJ, Schaffer JE, Lind AC, Miner JH
Proceedings of the National Academy of Sciences of the United States of America100(9)5274-9(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Med27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13859	NM_001290489.1 Latest version!	Mus musculus mediator complex subunit 27 (Med27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu17443	NM_029615.1 Latest version!	Mus musculus mediator complex subunit 27 (Med27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu80407	XM_017319246.1 Latest version!	Mus musculus mediator complex subunit 27 (Med27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu13867	NM_026896.5 Latest version!	Mus musculus mediator complex subunit 27 (Med27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu80408	NM_001290490.1 Latest version!	Mus musculus mediator complex subunit 27 (Med27), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu80408	XM_017319247.2 Latest version!	Mus musculus mediator complex subunit 27 (Med27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu80408	XM_017319247.1	Mus musculus mediator complex subunit 27 (Med27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med27 ( NM_001290489.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277418.1 Mus musculus mediator complex subunit 27 (Med27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu13859
Clone ID Related Accession (Same CDS sequence)	NM_001290489.1
Accession Version	NM_001290489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 27 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ073494.1, AL772213.7, AL929437.11 and AI850753.1. Transcript Variant: This variant (3) lacks two 5' exons but instead contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CX763452.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGTGT CTGCCAAGCG GAGACCGAAG GCCCAGCCCA CCACTCTGGT TCTACCACCT 
CAGTATGTCG ATGACGTGAT CAGCCGCATT GACAGAATGT TTCCAGAGAT GTCCATTCAC 
TTATCGAGAC CCAATGGGAC TTCAGCAATG CTTCTGGTGA CCTTGGGGAA GGTGTTGAAA 
GTCATTGTGG TCATGAGGAG CCTGTTCATC GATCGCACGA TTGTGAAGGG GTATAATGAG 
AGCGTCTACA CAGAGGATGG AAAGCTGGAC ATATGGTCCA AATCCAGCTA CCAAGTGTTC 
CAGAAGGTGA CAGACCATGC CACCACTGCC CTGCTTCACT ATCAGTTACC CCAGATGCCA 
GACGTCGTGG TCAGGTCCTT CATGACCTGG CTGAGAAGCT ACATTAAGCT GTTCCAGGCC 
CCGTGCCAGC GCTGTGGGAA GTTTCTACAA GATGGCCTTC CCCCGACGTG GAGAGACTTC 
CGAACCCTGG AAGCTTTCCA TGACACCTGC CGACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277418.1
CDS	179..697
Translation	MGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNESVYTEDGKLDIWSKSSYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	NM_001290489.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 27 (Med27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290489.1

ORF Insert Sequence:

ATGGGTGTGT CTGCCAAGCG GAGACCGAAG GCCCAGCCCA CCACTCTGGT TCTACCACCT 
CAGTATGTCG ATGACGTGAT CAGCCGCATT GACAGAATGT TTCCAGAGAT GTCCATTCAC 
TTATCGAGAC CCAATGGGAC TTCAGCAATG CTTCTGGTGA CCTTGGGGAA GGTGTTGAAA 
GTCATTGTGG TCATGAGGAG CCTGTTCATC GATCGCACGA TTGTGAAGGG GTATAATGAG 
AGCGTCTACA CAGAGGATGG AAAGCTGGAC ATATGGTCCA AATCCAGCTA CCAAGTGTTC 
CAGAAGGTGA CAGACCATGC CACCACTGCC CTGCTTCACT ATCAGTTACC CCAGATGCCA 
GACGTCGTGG TCAGGTCCTT CATGACCTGG CTGAGAAGCT ACATTAAGCT GTTCCAGGCC 
CCGTGCCAGC GCTGTGGGAA GTTTCTACAA GATGGCCTTC CCCCGACGTG GAGAGACTTC 
CGAACCCTGG AAGCTTTCCA TGACACCTGC CGACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med27 ( NM_029615.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083891.1 Mus musculus mediator complex subunit 27 (Med27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17443
Clone ID Related Accession (Same CDS sequence)	NM_029615.1
Accession Version	NM_029615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 27 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ072886.1, AK009764.1, AL929437.11 and AK147181.1. On Mar 13, 2014 this sequence version replaced XM_006498294.1. Transcript Variant: This variant (2) lacks four 3' exons but instead contains an alternate 3' terminal exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK147181.1, AK009764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG TGCTGAGCGT TGGTGTGAAC CTGGAGGCCT TTTCGCAGGC TATTAGCGCC 
ATCCAGGCGC TACGCTCTAG CGTGAGTAGG GTCTTCGACT GCCTTAAGGA TGGCATGCGC 
AACAAAGAGA CGCTGGAGGG CCGCGAGAAG GCCTTCATCG CGAACTTCCA GGACAACTTG 
CATTCCGTCA ACCGGGACCT CAATGAACTG GAACGTTTGA GCAACCTGGT AGGCAAGCCG 
TCTGAGAACC ACCCTCTTCA TAACAGTGGG CTGCTAAGCC TGGATCCTGT GCAGGACAAA 
ACCCCACTCT ACAGCCAGCT TCTGCAAGCA TACAAATGGT CCAACAAGCT GCAGTACCAT 
GCAGGTCTAG CGTCTGGCCT TTTGAACCAA CAGTCCTTGA AGCGCTCTGC AAATCAGATG 
GGTGTGTCTG CCAAGCGGAG ACCGAAGGCC CAGCCCACCA CTCTGGTTCT ACCACCTCAG 
TATGTCGATG ACGTGATCAG CCGCATTGAC AGAATGTTTC CAGAGATGTC CATTCACTTA 
TCGAGACCCA ATGGGACTTC AGCAATGCTT CTGATTCTTA TAAACAGCTC TGCTCAGAGC 
ATTACTGGCC CTTGGTTTTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083891.1
CDS	36..662
Translation	MADVLSVGVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIANFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLILINSSAQSITGPWFWS

Target ORF information:

RefSeq Version	NM_029615.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 27 (Med27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029615.1

ORF Insert Sequence:

ATGGCGGACG TGCTGAGCGT TGGTGTGAAC CTGGAGGCCT TTTCGCAGGC TATTAGCGCC 
ATCCAGGCGC TACGCTCTAG CGTGAGTAGG GTCTTCGACT GCCTTAAGGA TGGCATGCGC 
AACAAAGAGA CGCTGGAGGG CCGCGAGAAG GCCTTCATCG CGAACTTCCA GGACAACTTG 
CATTCCGTCA ACCGGGACCT CAATGAACTG GAACGTTTGA GCAACCTGGT AGGCAAGCCG 
TCTGAGAACC ACCCTCTTCA TAACAGTGGG CTGCTAAGCC TGGATCCTGT GCAGGACAAA 
ACCCCACTCT ACAGCCAGCT TCTGCAAGCA TACAAATGGT CCAACAAGCT GCAGTACCAT 
GCAGGTCTAG CGTCTGGCCT TTTGAACCAA CAGTCCTTGA AGCGCTCTGC AAATCAGATG 
GGTGTGTCTG CCAAGCGGAG ACCGAAGGCC CAGCCCACCA CTCTGGTTCT ACCACCTCAG 
TATGTCGATG ACGTGATCAG CCGCATTGAC AGAATGTTTC CAGAGATGTC CATTCACTTA 
TCGAGACCCA ATGGGACTTC AGCAATGCTT CTGATTCTTA TAAACAGCTC TGCTCAGAGC 
ATTACTGGCC CTTGGTTTTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med27 ( XM_017319246.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174735.1 Mus musculus mediator complex subunit 27 (Med27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80407
Clone ID Related Accession (Same CDS sequence)	XM_017319246.1
Accession Version	XM_017319246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT ACCATGCAGG TCTAGCGTCT GGCCTTTTGA ACCAACAGTC CTTGAAGCGC 
TCTGCAAATC AGATGGGTGT GTCTGCCAAG CGGAGACCGA AGGCCCAGCC CACCACTCTG 
GTTCTACCAC CTCAGTATGT CGATGACGTG ATCAGCCGCA TTGACAGAAT GTTTCCAGAG 
ATGTCCATTC ACTTATCGAG ACCCAATGGG ACTTCAGCAA TGCTTCTGGT GACCTTGGGG 
AAGGTGTTGA AAGTCATTGT GGTCATGAGG AGCCTGTTCA TCGATCGCAC GATTGTGAAG 
GGGTATAATG AGAGCGTCTA CACAGAGGAT GGAAAGCTGG ACATATGGTC CAAATCCAGC 
TACCAAGTGT TCCAGAAGGT GACAGACCAT GCCACCACTG CCCTGCTTCA CTATCAGTTA 
CCCCAGATGC CAGACGTCGT GGTCAGGTCC TTCATGACCT GGCTGAGAAG CTACATTAAG 
CTGTTCCAGG CCCCGTGCCA GCGCTGTGGG AAGTTTCTAC AAGATGGCCT TCCCCCGACG 
TGGAGAGACT TCCGAACCCT GGAAGCTTTC CATGACACCT GCCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174735.1
CDS	20..610
Translation	MLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNESVYTEDGKLDIWSKSSYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	XM_017319246.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 27 (Med27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319246.1

ORF Insert Sequence:

ATGCTGCAGT ACCATGCAGG TCTAGCGTCT GGCCTTTTGA ACCAACAGTC CTTGAAGCGC 
TCTGCAAATC AGATGGGTGT GTCTGCCAAG CGGAGACCGA AGGCCCAGCC CACCACTCTG 
GTTCTACCAC CTCAGTATGT CGATGACGTG ATCAGCCGCA TTGACAGAAT GTTTCCAGAG 
ATGTCCATTC ACTTATCGAG ACCCAATGGG ACTTCAGCAA TGCTTCTGGT GACCTTGGGG 
AAGGTGTTGA AAGTCATTGT GGTCATGAGG AGCCTGTTCA TCGATCGCAC GATTGTGAAG 
GGGTATAATG AGAGCGTCTA CACAGAGGAT GGAAAGCTGG ACATATGGTC CAAATCCAGC 
TACCAAGTGT TCCAGAAGGT GACAGACCAT GCCACCACTG CCCTGCTTCA CTATCAGTTA 
CCCCAGATGC CAGACGTCGT GGTCAGGTCC TTCATGACCT GGCTGAGAAG CTACATTAAG 
CTGTTCCAGG CCCCGTGCCA GCGCTGTGGG AAGTTTCTAC AAGATGGCCT TCCCCCGACG 
TGGAGAGACT TCCGAACCCT GGAAGCTTTC CATGACACCT GCCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med27 ( NM_026896.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081172.2 Mus musculus mediator complex subunit 27 (Med27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13867
Clone ID Related Accession (Same CDS sequence)	NM_026896.5
Accession Version	NM_026896.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 27 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ072886.1, AK005251.1 and AI850753.1. On Mar 13, 2014 this sequence version replaced NM_026896.4. Transcript Variant: This variant (1) encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC016537.1, AK005251.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG TGCTGAGCGT TGGTGTGAAC CTGGAGGCCT TTTCGCAGGC TATTAGCGCC 
ATCCAGGCGC TACGCTCTAG CGTGAGTAGG GTCTTCGACT GCCTTAAGGA TGGCATGCGC 
AACAAAGAGA CGCTGGAGGG CCGCGAGAAG GCCTTCATCG CGAACTTCCA GGACAACTTG 
CATTCCGTCA ACCGGGACCT CAATGAACTG GAACGTTTGA GCAACCTGGT AGGCAAGCCG 
TCTGAGAACC ACCCTCTTCA TAACAGTGGG CTGCTAAGCC TGGATCCTGT GCAGGACAAA 
ACCCCACTCT ACAGCCAGCT TCTGCAAGCA TACAAATGGT CCAACAAGCT GCAGTACCAT 
GCAGGTCTAG CGTCTGGCCT TTTGAACCAA CAGTCCTTGA AGCGCTCTGC AAATCAGATG 
GGTGTGTCTG CCAAGCGGAG ACCGAAGGCC CAGCCCACCA CTCTGGTTCT ACCACCTCAG 
TATGTCGATG ACGTGATCAG CCGCATTGAC AGAATGTTTC CAGAGATGTC CATTCACTTA 
TCGAGACCCA ATGGGACTTC AGCAATGCTT CTGGTGACCT TGGGGAAGGT GTTGAAAGTC 
ATTGTGGTCA TGAGGAGCCT GTTCATCGAT CGCACGATTG TGAAGGGGTA TAATGAGAGC 
GTCTACACAG AGGATGGAAA GCTGGACATA TGGTCCAAAT CCAGCTACCA AGTGTTCCAG 
AAGGTGACAG ACCATGCCAC CACTGCCCTG CTTCACTATC AGTTACCCCA GATGCCAGAC 
GTCGTGGTCA GGTCCTTCAT GACCTGGCTG AGAAGCTACA TTAAGCTGTT CCAGGCCCCG 
TGCCAGCGCT GTGGGAAGTT TCTACAAGAT GGCCTTCCCC CGACGTGGAG AGACTTCCGA 
ACCCTGGAAG CTTTCCATGA CACCTGCCGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081172.2
CDS	36..971
Translation	MADVLSVGVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIANFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNESVYTEDGKLDIWSKSSYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	NM_026896.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 27 (Med27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026896.5

ORF Insert Sequence:

ATGGCGGACG TGCTGAGCGT TGGTGTGAAC CTGGAGGCCT TTTCGCAGGC TATTAGCGCC 
ATCCAGGCGC TACGCTCTAG CGTGAGTAGG GTCTTCGACT GCCTTAAGGA TGGCATGCGC 
AACAAAGAGA CGCTGGAGGG CCGCGAGAAG GCCTTCATCG CGAACTTCCA GGACAACTTG 
CATTCCGTCA ACCGGGACCT CAATGAACTG GAACGTTTGA GCAACCTGGT AGGCAAGCCG 
TCTGAGAACC ACCCTCTTCA TAACAGTGGG CTGCTAAGCC TGGATCCTGT GCAGGACAAA 
ACCCCACTCT ACAGCCAGCT TCTGCAAGCA TACAAATGGT CCAACAAGCT GCAGTACCAT 
GCAGGTCTAG CGTCTGGCCT TTTGAACCAA CAGTCCTTGA AGCGCTCTGC AAATCAGATG 
GGTGTGTCTG CCAAGCGGAG ACCGAAGGCC CAGCCCACCA CTCTGGTTCT ACCACCTCAG 
TATGTCGATG ACGTGATCAG CCGCATTGAC AGAATGTTTC CAGAGATGTC CATTCACTTA 
TCGAGACCCA ATGGGACTTC AGCAATGCTT CTGGTGACCT TGGGGAAGGT GTTGAAAGTC 
ATTGTGGTCA TGAGGAGCCT GTTCATCGAT CGCACGATTG TGAAGGGGTA TAATGAGAGC 
GTCTACACAG AGGATGGAAA GCTGGACATA TGGTCCAAAT CCAGCTACCA AGTGTTCCAG 
AAGGTGACAG ACCATGCCAC CACTGCCCTG CTTCACTATC AGTTACCCCA GATGCCAGAC 
GTCGTGGTCA GGTCCTTCAT GACCTGGCTG AGAAGCTACA TTAAGCTGTT CCAGGCCCCG 
TGCCAGCGCT GTGGGAAGTT TCTACAAGAT GGCCTTCCCC CGACGTGGAG AGACTTCCGA 
ACCCTGGAAG CTTTCCATGA CACCTGCCGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med27 ( NM_001290490.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277419.1 Mus musculus mediator complex subunit 27 (Med27), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu80408
Clone ID Related Accession (Same CDS sequence)	XM_017319247.2 , XM_017319247.1 , NM_001290490.1
Accession Version	NM_001290490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 27 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ073494.1, DV648756.1, AL929437.11 and AK147181.1. Transcript Variant: This variant (4) contains alternate 5' and 3' exon structure, and it thus differs in both UTRs and in the 5' and 3' coding regions, compared to variant 1. The encoded isoform (d) has distinct N- and C-termini and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: DV648756.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGT ACCATGCAGG TCTAGCGTCT GGCCTTTTGA ACCAACAGTC CTTGAAGCGC 
TCTGCAAATC AGATGGGTGT GTCTGCCAAG CGGAGACCGA AGGCCCAGCC CACCACTCTG 
GTTCTACCAC CTCAGTATGT CGATGACGTG ATCAGCCGCA TTGACAGAAT GTTTCCAGAG 
ATGTCCATTC ACTTATCGAG ACCCAATGGG ACTTCAGCAA TGCTTCTGAT TCTTATAAAC 
AGCTCTGCTC AGAGCATTAC TGGCCCTTGG TTTTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277419.1
CDS	239..520
Translation	MLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLILINSSAQSITGPWFWS

Target ORF information:

RefSeq Version	NM_001290490.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 27 (Med27), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290490.1

ORF Insert Sequence:

ATGCTGCAGT ACCATGCAGG TCTAGCGTCT GGCCTTTTGA ACCAACAGTC CTTGAAGCGC 
TCTGCAAATC AGATGGGTGT GTCTGCCAAG CGGAGACCGA AGGCCCAGCC CACCACTCTG 
GTTCTACCAC CTCAGTATGT CGATGACGTG ATCAGCCGCA TTGACAGAAT GTTTCCAGAG 
ATGTCCATTC ACTTATCGAG ACCCAATGGG ACTTCAGCAA TGCTTCTGAT TCTTATAAAC 
AGCTCTGCTC AGAGCATTAC TGGCCCTTGG TTTTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med27 ( XM_017319247.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174736.1 Mus musculus mediator complex subunit 27 (Med27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu80408
Clone ID Related Accession (Same CDS sequence)	XM_017319247.2 , XM_017319247.1 , NM_001290490.1
Accession Version	XM_017319247.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT ACCATGCAGG TCTAGCGTCT GGCCTTTTGA ACCAACAGTC CTTGAAGCGC 
TCTGCAAATC AGATGGGTGT GTCTGCCAAG CGGAGACCGA AGGCCCAGCC CACCACTCTG 
GTTCTACCAC CTCAGTATGT CGATGACGTG ATCAGCCGCA TTGACAGAAT GTTTCCAGAG 
ATGTCCATTC ACTTATCGAG ACCCAATGGG ACTTCAGCAA TGCTTCTGAT TCTTATAAAC 
AGCTCTGCTC AGAGCATTAC TGGCCCTTGG TTTTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174736.1
CDS	3850..4131
Translation	MLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLILINSSAQSITGPWFWS

Target ORF information:

RefSeq Version	XM_017319247.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 27 (Med27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319247.2

ORF Insert Sequence:

ATGCTGCAGT ACCATGCAGG TCTAGCGTCT GGCCTTTTGA ACCAACAGTC CTTGAAGCGC 
TCTGCAAATC AGATGGGTGT GTCTGCCAAG CGGAGACCGA AGGCCCAGCC CACCACTCTG 
GTTCTACCAC CTCAGTATGT CGATGACGTG ATCAGCCGCA TTGACAGAAT GTTTCCAGAG 
ATGTCCATTC ACTTATCGAG ACCCAATGGG ACTTCAGCAA TGCTTCTGAT TCTTATAAAC 
AGCTCTGCTC AGAGCATTAC TGGCCCTTGG TTTTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med27 ( XM_017319247.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174736.1 Mus musculus mediator complex subunit 27 (Med27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu80408
Clone ID Related Accession (Same CDS sequence)	XM_017319247.2 , XM_017319247.1 , NM_001290490.1
Accession Version	XM_017319247.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT ACCATGCAGG TCTAGCGTCT GGCCTTTTGA ACCAACAGTC CTTGAAGCGC 
TCTGCAAATC AGATGGGTGT GTCTGCCAAG CGGAGACCGA AGGCCCAGCC CACCACTCTG 
GTTCTACCAC CTCAGTATGT CGATGACGTG ATCAGCCGCA TTGACAGAAT GTTTCCAGAG 
ATGTCCATTC ACTTATCGAG ACCCAATGGG ACTTCAGCAA TGCTTCTGAT TCTTATAAAC 
AGCTCTGCTC AGAGCATTAC TGGCCCTTGG TTTTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174736.1
CDS	1613..1894
Translation	MLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLILINSSAQSITGPWFWS

Target ORF information:

RefSeq Version	XM_017319247.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 27 (Med27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319247.1

ORF Insert Sequence:

ATGCTGCAGT ACCATGCAGG TCTAGCGTCT GGCCTTTTGA ACCAACAGTC CTTGAAGCGC 
TCTGCAAATC AGATGGGTGT GTCTGCCAAG CGGAGACCGA AGGCCCAGCC CACCACTCTG 
GTTCTACCAC CTCAGTATGT CGATGACGTG ATCAGCCGCA TTGACAGAAT GTTTCCAGAG 
ATGTCCATTC ACTTATCGAG ACCCAATGGG ACTTCAGCAA TGCTTCTGAT TCTTATAAAC 
AGCTCTGCTC AGAGCATTAC TGGCCCTTGG TTTTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mediator and cohesin connect gene expression and chromatin architecture.
Nature467(7314)430-5(2010 Sep)
Kagey MH,Newman JJ,Bilodeau S,Zhan Y,Orlando DA,van Berkum NL,Ebmeier CC,Goossens J,Rahl PB,Levine SS,Taatjes DJ,Dekker J,Young RA

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
EMBO reports11(7)541-7(2010 Jul)
Krebs AR,Demmers J,Karmodiya K,Chang NC,Chang AC,Tora L

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Genes & development20(19)2713-27(2006 Oct)
Evsikov AV,Graber JH,Brockman JM,Hampl A,Holbrook AE,Singh P,Eppig JJ,Solter D,Knowles BB

Cloning of wrinkle-free, a previously uncharacterized mouse mutation, reveals crucial roles for fatty acid transport protein 4 in skin and hair development.
Proceedings of the National Academy of Sciences of the United States of America100(9)5274-9(2003 Apr)
Moulson CL,Martin DR,Lugus JJ,Schaffer JE,Lind AC,Miner JH

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Med27 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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