Prpf6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prpf6
Entrez Gene ID	68879
Full Name	pre-mRNA splicing factor 6
Synonyms	1190003A07Rik,2610031L17Rik,ANT-1,U5-102K
General protein information	Preferred Names pre-mRNA splicing factor 6 Names pre-mRNA-processing factor 6 PRP6 homolog PRP6 pre-mRNA splicing factor 6 homolog U5 snRNP-associated 102 kDa protein U5-102 kDa protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H4

mRNA and Protein(s)

mRNA	Protein	Name
NM_133701.2	NP_598462.1	pre-mRNA-processing factor 6

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRPF6	NP_036601.2
Rattus norvegicus (Norway rat)	Prpf6	NP_001073234.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005640	XP_315659.1
Oryza sativa (rice)	Os10g0498600	NP_001064969.1
Xenopus tropicalis (tropical clawed frog)	prpf6	NP_001025642.1
Canis lupus familiaris (dog)	PRPF6	XP_534481.3
Mus musculus (house mouse)	Prpf6	NP_598462.1
Gallus gallus (chicken)	PRPF6	XP_417426.2
Magnaporthe oryzae (rice blast fungus)	MGG_07213	XP_003715426.1
Arabidopsis thaliana (thale cress)	EMB2770	NP_192252.1
Pan troglodytes (chimpanzee)	PRPF6	XP_001152053.3
Bos taurus (cattle)	PRPF6	NP_001039840.1
Danio rerio (zebrafish)	prpf6	NP_997820.1
Schizosaccharomyces pombe (fission yeast)	prp1	NP_596086.1
Caenorhabditis elegans (roundworm)	prp-6	NP_507525.1
Drosophila melanogaster (fruit fly)	CG6841	NP_649073.1
Neurospora crassa	NCU04606	XP_958849.1

Gene Ontology
Bibliography

Related articles in PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Identification of the functional domains of ANT-1, a novel coactivator of the androgen receptor.
Fan S, Goto K, Chen G, Morinaga H, Nomura M, Okabe T, Nawata H, Yanase T
Biochemical and biophysical research communications341(1)192-201(2006 Mar)

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
McKee AE, Minet E, Stern C, Riahi S, Stiles CD, Silver PA
BMC developmental biology514(2005 Jul)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The existence of these functional domains may further support the idea that ANT-1 can function as an AR-AF-1-specific coactivator while mediating a transcription-splicing coupling.
Title: Identification of the functional domains of ANT-1, a novel coactivator of the androgen receptor.

The following Prpf6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10108	NM_133701.2 Latest version!	Mus musculus pre-mRNA splicing factor 6 (Prpf6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$552.30 $789.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prpf6 ( NM_133701.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598462.1 Mus musculus pre-mRNA splicing factor 6 (Prpf6), mRNA.

Price & Availability↑

CloneID	OMu10108
Clone ID Related Accession (Same CDS sequence)	NM_133701.2
Accession Version	NM_133701.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	pre-mRNA-processing factor 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY735790.1, AK081998.1 and CJ228927.1. On Jan 23, 2007 this sequence version replaced NM_133701.1. ##Evidence-Data-START## Transcript exon combination :: BC014869.1, AK145461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGAACAAGA AGAAGAAGCC GTTCCTGGGC ATGCCTGCGC CGCTGGGCTA CGTACCCGGG 
CTGGGCAGGG GTGCCACTGG CTTCACCACC CGATCAGACA TCGGGCCTGC TCGGGATGCA 
AATGATCCTG TGGATGATCG CCATGCCCCA CCAGGGAAGA GAACTGTGGG GGACCAGATG 
AAGAAAAATC AGGCGGCTGA TGATGATGAT GAAGATCTGA ATGATACCAA CTACGATGAG 
TTTAATGGCT ATGCTGGGAG CCTCTTCTCA AGTGGGCCCT ATGAAAAAGA TGATGAGGAA 
GCAGATGCTA TTTATGCAGC TCTGGATAAA AGGATGGATG AGAGAAGAAA AGAGAGAAGA 
GAGCAAAGGG AGAAAGAAGA GATTGAGAAA TACCGAATGG AACGTCCCAA AATCCAGCAG 
CAATTTTCAG ACCTTAAGAG GAAGCTGGCA GAAGTCACAG AAGAAGAGTG GCTGAGCATC 
CCAGAAGTTG GCGATGCCAG AAACAAACGT CAACGGAACC CACGTTATGA GAAGCTGACT 
CCTGTTCCTG ATAGTTTCTT TGCCAAACAT TTACAGACTG GAGAGAACCA TACTTCTGTG 
GATCCCCGAC AGACTCAATT TGGAGGTCTG AACACACCCT ACCCGGGAGG CCTGAACACT 
CCATACCCAG GTGGAATGAC ACCTGGATTG ATGACACCTG GTACAGGGGA GCTGGACATG 
AGGAAGATTG GCCAGGCAAG GAATACCCTG ATGGACATGA GGCTTAGCCA GGTATCTGAC 
TCAGTGAGTG GGCAGACTGT TGTGGACCCC AAAGGCTACC TGACAGATCT AAATTCCATG 
ATCCCAACCC ATGGCGGGGA CATAAATGAC ATCAAGAAAG CACGACTACT TCTCAAATCT 
GTTCGAGAAA CAAACCCTCA TCACCCACCA GCTTGGATTG CATCAGCTCG CCTGGAAGAA 
GTCACTGGAA AGTTACAAGT AGCTCGAAAC CTTATTATGA AAGGGACAGA GATGTGCCCC 
AAGAGTGAAG ATGTCTGGCT AGAAGCAGCT AGGTTACAGC CTGGGGACAC AGCCAAGGCT 
GTGGTGGCAC AAGCTGTCCG TCATCTCCCG CAGTCTGTTA GGATTTACAT CAGAGCAGCA 
GAGCTAGAAA CAGACATTCG AGCAAAGAAG CGTGTTCTTC GGAAAGCACT TGAGCATGTT 
CCAAACTCTG TTCGATTGTG GAAAGCAGCT GTTGAGCTTG AAGAGCCTGA AGATGCTAGA 
ATCATGCTGA GCCGAGCAGT AGAGTGCTGT CCCACCAGTG TAGAGCTATG GCTTGCTTTG 
GCAAGACTAG AAACTTATGA AAATGCTCGC AAGGTCTTAA ATAAAGCACG TGAGAACATT 
CCTACAGATC GGCACATCTG GATCACAGCT GCCAAACTGG AGGAGGCCAA TGGGAACACT 
CAGATGGTGG AAAAGATCAT TGACCGAGCC ATCACCTCCC TACGGGCCAA TGGTGTAGAG 
ATCAACCGCG AACAGTGGAT CCAGGATGCT GAGGAGTGTG ACAGGGCAGG GAGTGTGGCC 
ACGTGCCAGG CTGTCATGCG TGCTGTAATC GGTATTGGGA TTGAGGAGGA AGATCGCAAA 
CACACCTGGA TGGAAGACGC TGACAGTTGT GTGGCCCACA ATGCCCTGGA GTGTGCACGA 
GCCATATATG CCTATGCCCT ACAAGTCTTC CCCAGCAAGA AAAGTGTGTG GTTACGAGCT 
GCCTACTTTG AGAAGAATCA TGGTACCAGG GAGTCTCTGG AAGCACTCTT GCAGAGGGCT 
GTAGCCCACT GCCCCAAGGC AGAGGTGCTA TGGCTCATGG GTGCCAAGTC AAAGTGGCTG 
GCAGGGGATG TACCTGCAGC AAGGAGCATC CTGGCCCTGG CCTTTCAGGC CAACCCCAAC 
AGCGAAGAGA TCTGGTTGGC GGCTGTGAAG CTGGAGTCAG AGAACAATGA ATACGAGCGA 
GCCCGGAGGC TACTGGCCAA GGCTCGGAGC AGCGCACCCA CTGCCCGTGT ATTCATGAAG 
TCTGTGAAGC TGGAGTGGGT TCTGGGGAAC ATCTCTGCGG CGCAGGAGCT GTGTGAGGAG 
GCTCTGAGGC ACTATGAAGA TTTCCCCAAG CTGTGGATGA TGAAGGGGCA GATTGAGGAG 
CAGGGAGAAC TGATGGAGAA GGCCCGGGAG GCCTACAACC AGGGGCTGAA GAAATGTCCT 
CATTCCACAC CGCTTTGGCT CTTGCTTTCC CGACTAGAGG AAAAGATTGG GCAGCTGACA 
CGTGCTCGGG CCATTTTAGA GAAATCTCGT CTGAAAAACC CAAAGAACCC TGGGCTATGG 
TTGGAGTCAG TGCGGCTGGA GTACCGGGCA GGGCTGAAGA ACATTGCCAA TACCCTCATG 
GCCAAGGCGC TGCAGGAGTG CCCTAACTCT GGTATCCTGT GGTCTGAAGC TGTCTTCCTT 
GAGGCAAGGC CCCAGAGAAA GACCAAGAGT GTGGATGCCC TGAAGAAGTG TGAACATGAC 
CCCCATGTGC TTTTAGCTGT GGCTAAGTTG TTCTGGAGTG AAAGAAAGAT CACCAAGGCT 
CGGGAGTGGT TCCATCGCAC TGTGAAGATT GACTCAGATC TGGGGGATGC CTGGGCCTTC 
TTCTACAAAT TTGAACTGCA GCATGGAACT GAGGAGCAAC AGGAGGAGGT GAGGAAACGC 
TGTGAGAATG CAGAGCCCCG ACATGGAGAG CTGTGGTGTG CTGTGTCCAA GGATATCACC 
AACTGGCAGA GGAAGATTGG GGAAATCCTA GTCCTGGTGG CCGCCCGCAT CAAAAATACC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598462.1
CDS	143..2968
Translation	MNKKKKPFLGMPAPLGYVPGLGRGATGFTTRSDIGPARDANDPVDDRHAPPGKRTVGDQMKKNQAADDDDEDLNDTNYDEFNGYAGSLFSSGPYEKDDEEADAIYAALDKRMDERRKERREQREKEEIEKYRMERPKIQQQFSDLKRKLAEVTEEEWLSIPEVGDARNKRQRNPRYEKLTPVPDSFFAKHLQTGENHTSVDPRQTQFGGLNTPYPGGLNTPYPGGMTPGLMTPGTGELDMRKIGQARNTLMDMRLSQVSDSVSGQTVVDPKGYLTDLNSMIPTHGGDINDIKKARLLLKSVRETNPHHPPAWIASARLEEVTGKLQVARNLIMKGTEMCPKSEDVWLEAARLQPGDTAKAVVAQAVRHLPQSVRIYIRAAELETDIRAKKRVLRKALEHVPNSVRLWKAAVELEEPEDARIMLSRAVECCPTSVELWLALARLETYENARKVLNKARENIPTDRHIWITAAKLEEANGNTQMVEKIIDRAITSLRANGVEINREQWIQDAEECDRAGSVATCQAVMRAVIGIGIEEEDRKHTWMEDADSCVAHNALECARAIYAYALQVFPSKKSVWLRAAYFEKNHGTRESLEALLQRAVAHCPKAEVLWLMGAKSKWLAGDVPAARSILALAFQANPNSEEIWLAAVKLESENNEYERARRLLAKARSSAPTARVFMKSVKLEWVLGNISAAQELCEEALRHYEDFPKLWMMKGQIEEQGELMEKAREAYNQGLKKCPHSTPLWLLLSRLEEKIGQLTRARAILEKSRLKNPKNPGLWLESVRLEYRAGLKNIANTLMAKALQECPNSGILWSEAVFLEARPQRKTKSVDALKKCEHDPHVLLAVAKLFWSERKITKAREWFHRTVKIDSDLGDAWAFFYKFELQHGTEEQQEEVRKRCENAEPRHGELWCAVSKDITNWQRKIGEILVLVAARIKNTF

Target ORF information:

RefSeq Version	NM_133701.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA splicing factor 6 (Prpf6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133701.2

ORF Insert Sequence:

ATGAACAAGA AGAAGAAGCC GTTCCTGGGC ATGCCTGCGC CGCTGGGCTA CGTACCCGGG 
CTGGGCAGGG GTGCCACTGG CTTCACCACC CGATCAGACA TCGGGCCTGC TCGGGATGCA 
AATGATCCTG TGGATGATCG CCATGCCCCA CCAGGGAAGA GAACTGTGGG GGACCAGATG 
AAGAAAAATC AGGCGGCTGA TGATGATGAT GAAGATCTGA ATGATACCAA CTACGATGAG 
TTTAATGGCT ATGCTGGGAG CCTCTTCTCA AGTGGGCCCT ATGAAAAAGA TGATGAGGAA 
GCAGATGCTA TTTATGCAGC TCTGGATAAA AGGATGGATG AGAGAAGAAA AGAGAGAAGA 
GAGCAAAGGG AGAAAGAAGA GATTGAGAAA TACCGAATGG AACGTCCCAA AATCCAGCAG 
CAATTTTCAG ACCTTAAGAG GAAGCTGGCA GAAGTCACAG AAGAAGAGTG GCTGAGCATC 
CCAGAAGTTG GCGATGCCAG AAACAAACGT CAACGGAACC CACGTTATGA GAAGCTGACT 
CCTGTTCCTG ATAGTTTCTT TGCCAAACAT TTACAGACTG GAGAGAACCA TACTTCTGTG 
GATCCCCGAC AGACTCAATT TGGAGGTCTG AACACACCCT ACCCGGGAGG CCTGAACACT 
CCATACCCAG GTGGAATGAC ACCTGGATTG ATGACACCTG GTACAGGGGA GCTGGACATG 
AGGAAGATTG GCCAGGCAAG GAATACCCTG ATGGACATGA GGCTTAGCCA GGTATCTGAC 
TCAGTGAGTG GGCAGACTGT TGTGGACCCC AAAGGCTACC TGACAGATCT AAATTCCATG 
ATCCCAACCC ATGGCGGGGA CATAAATGAC ATCAAGAAAG CACGACTACT TCTCAAATCT 
GTTCGAGAAA CAAACCCTCA TCACCCACCA GCTTGGATTG CATCAGCTCG CCTGGAAGAA 
GTCACTGGAA AGTTACAAGT AGCTCGAAAC CTTATTATGA AAGGGACAGA GATGTGCCCC 
AAGAGTGAAG ATGTCTGGCT AGAAGCAGCT AGGTTACAGC CTGGGGACAC AGCCAAGGCT 
GTGGTGGCAC AAGCTGTCCG TCATCTCCCG CAGTCTGTTA GGATTTACAT CAGAGCAGCA 
GAGCTAGAAA CAGACATTCG AGCAAAGAAG CGTGTTCTTC GGAAAGCACT TGAGCATGTT 
CCAAACTCTG TTCGATTGTG GAAAGCAGCT GTTGAGCTTG AAGAGCCTGA AGATGCTAGA 
ATCATGCTGA GCCGAGCAGT AGAGTGCTGT CCCACCAGTG TAGAGCTATG GCTTGCTTTG 
GCAAGACTAG AAACTTATGA AAATGCTCGC AAGGTCTTAA ATAAAGCACG TGAGAACATT 
CCTACAGATC GGCACATCTG GATCACAGCT GCCAAACTGG AGGAGGCCAA TGGGAACACT 
CAGATGGTGG AAAAGATCAT TGACCGAGCC ATCACCTCCC TACGGGCCAA TGGTGTAGAG 
ATCAACCGCG AACAGTGGAT CCAGGATGCT GAGGAGTGTG ACAGGGCAGG GAGTGTGGCC 
ACGTGCCAGG CTGTCATGCG TGCTGTAATC GGTATTGGGA TTGAGGAGGA AGATCGCAAA 
CACACCTGGA TGGAAGACGC TGACAGTTGT GTGGCCCACA ATGCCCTGGA GTGTGCACGA 
GCCATATATG CCTATGCCCT ACAAGTCTTC CCCAGCAAGA AAAGTGTGTG GTTACGAGCT 
GCCTACTTTG AGAAGAATCA TGGTACCAGG GAGTCTCTGG AAGCACTCTT GCAGAGGGCT 
GTAGCCCACT GCCCCAAGGC AGAGGTGCTA TGGCTCATGG GTGCCAAGTC AAAGTGGCTG 
GCAGGGGATG TACCTGCAGC AAGGAGCATC CTGGCCCTGG CCTTTCAGGC CAACCCCAAC 
AGCGAAGAGA TCTGGTTGGC GGCTGTGAAG CTGGAGTCAG AGAACAATGA ATACGAGCGA 
GCCCGGAGGC TACTGGCCAA GGCTCGGAGC AGCGCACCCA CTGCCCGTGT ATTCATGAAG 
TCTGTGAAGC TGGAGTGGGT TCTGGGGAAC ATCTCTGCGG CGCAGGAGCT GTGTGAGGAG 
GCTCTGAGGC ACTATGAAGA TTTCCCCAAG CTGTGGATGA TGAAGGGGCA GATTGAGGAG 
CAGGGAGAAC TGATGGAGAA GGCCCGGGAG GCCTACAACC AGGGGCTGAA GAAATGTCCT 
CATTCCACAC CGCTTTGGCT CTTGCTTTCC CGACTAGAGG AAAAGATTGG GCAGCTGACA 
CGTGCTCGGG CCATTTTAGA GAAATCTCGT CTGAAAAACC CAAAGAACCC TGGGCTATGG 
TTGGAGTCAG TGCGGCTGGA GTACCGGGCA GGGCTGAAGA ACATTGCCAA TACCCTCATG 
GCCAAGGCGC TGCAGGAGTG CCCTAACTCT GGTATCCTGT GGTCTGAAGC TGTCTTCCTT 
GAGGCAAGGC CCCAGAGAAA GACCAAGAGT GTGGATGCCC TGAAGAAGTG TGAACATGAC 
CCCCATGTGC TTTTAGCTGT GGCTAAGTTG TTCTGGAGTG AAAGAAAGAT CACCAAGGCT 
CGGGAGTGGT TCCATCGCAC TGTGAAGATT GACTCAGATC TGGGGGATGC CTGGGCCTTC 
TTCTACAAAT TTGAACTGCA GCATGGAACT GAGGAGCAAC AGGAGGAGGT GAGGAAACGC 
TGTGAGAATG CAGAGCCCCG ACATGGAGAG CTGTGGTGTG CTGTGTCCAA GGATATCACC 
AACTGGCAGA GGAAGATTGG GGAAATCCTA GTCCTGGTGG CCGCCCGCAT CAAAAATACC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Identification of the functional domains of ANT-1, a novel coactivator of the androgen receptor.
Biochemical and biophysical research communications341(1)192-201(2006 Mar)
Fan S,Goto K,Chen G,Morinaga H,Nomura M,Okabe T,Nawata H,Yanase T

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
BMC developmental biology514(2005 Jul)
McKee AE,Minet E,Stern C,Riahi S,Stiles CD,Silver PA

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Prpf6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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