Smtnl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smtnl1
Entrez Gene ID	68678
Full Name	smoothelin-like 1
Synonyms	1110030K22Rik,CHASM
General protein information	Preferred Names smoothelin-like 1 Names smoothelin-like protein 1 calponin homology-associated smooth muscle protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006500109.3	XP_006500172.1	smoothelin-like protein 1 isoform X1
XM_006500109.4	XP_006500172.1	smoothelin-like protein 1 isoform X1
NM_024230.2	NP_077192.1	smoothelin-like protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMTNL1	XP_508434.3
	SMTNL1	XP_001092494.2
Canis lupus familiaris (dog)	SMTNL1	XP_005631261.1
Mus musculus (house mouse)	Smtnl1	NP_077192.1
Rattus norvegicus (Norway rat)	Smtnl1	NP_001178668.1
Homo sapiens (human)	SMTNL1	NP_001099035.2
Bos taurus (cattle)	SMTNL1	XP_002707777.1

Gene Ontology
Bibliography

Related articles in PubMed

Pregnancy and Smoothelin-like Protein 1 (SMTNL1) Deletion Promote the Switching of Skeletal Muscle to a Glycolytic Phenotype in Human and Mice.
Lontay B, Bodoor K, Sipos A, Weitzel DH, Loiselle D, Safi R, Zheng D, Devente J, Hickner RC, McDonnell DP, Ribar T, Haystead TA
The Journal of biological chemistry290(29)17985-98(2015 Jul)

Two domains of the smoothelin-like 1 protein bind apo- and calcium-calmodulin independently.
Ulke-Lem?e A, Ishida H, Chappellaz M, Vogel HJ, MacDonald JA
Biochimica et biophysica acta1844(9)1580-90(2014 Sep)

Intrinsically disordered N-terminus of calponin homology-associated smooth muscle protein (CHASM) interacts with the calponin homology domain to enable tropomyosin binding.
MacDonald JA, Ishida H, Butler EI, Ulke-Lem?e A, Chappellaz M, Tulk SE, Chik JK, Vogel HJ
Biochemistry51(13)2694-705(2012 Apr)

Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression.
Richard AF, Demignon J, Sakakibara I, Pujol J, Favier M, Strochlic L, Le Grand F, Sgarioto N, Guernec A, Schmitt A, Cagnard N, Huang R, Legay C, Guillet-Deniau I, Maire P
Developmental biology359(2)303-20(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pregnancy promotes switching of skeletal muscle to a glycolytic phenotype through the smoothelin-like protein 1 transcriptional cofactor.
Title: Pregnancy and Smoothelin-like Protein 1 (SMTNL1) Deletion Promote the Switching of Skeletal Muscle to a Glycolytic Phenotype in Human and Mice.

Two domains of SMTNL1 bind apo- and calcium-calmodulin independently.
Title: Two domains of the smoothelin-like 1 protein bind apo- and calcium-calmodulin independently.

appears that the type-2 CH domain of CHASM is required for tropomyosin binding and presents a novel function for this protein domain
Title: Intrinsically disordered N-terminus of calponin homology-associated smooth muscle protein (CHASM) interacts with the calponin homology domain to enable tropomyosin binding.

SMTNL1 is a bifunctional co-regulator of PR-B signaling and provides a molecular mechanism whereby PR-B is targeted to alter gene expression patterns within uterine smooth muscle cells to coordinately promote alterations in USM function during pregnancy
Title: Smoothelin-like 1 protein is a bifunctional regulator of the progesterone receptor during pregnancy.

The Smtnl1 proximal promoter enhances expression up to 8-fold in smooth muscle cells and a second activating region lays 500 bp further upstream.
Title: Mapping and functional characterization of the murine smoothelin-like 1 promoter.

The following Smtnl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smtnl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16300	NM_024230.2 Latest version!	Mus musculus smoothelin-like 1 (Smtnl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu16300	XM_006500109.4 Latest version!	Mus musculus smoothelin-like 1 (Smtnl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu16300	XM_006500109.3	Mus musculus smoothelin-like 1 (Smtnl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smtnl1 ( NM_024230.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077192.1 Mus musculus smoothelin-like 1 (Smtnl1), mRNA.

Price & Availability↑

CloneID	OMu16300
Clone ID Related Accession (Same CDS sequence)	XM_006500109.3 , XM_006500109.4 , NM_024230.2
Accession Version	NM_024230.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	smoothelin-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL928914.15. On Nov 17, 2006 this sequence version replaced NM_024230.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002317.1, AK003989.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849376 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCAGA CAGAAGGGAA CTCCTCTGAG GATGGGACCA CTGTCTCCCC AACTGCAGGG 
AATCTTGAGA CACCAGGAAG TCAAGGCATT GCAGAGGAGG TGGCCGAAGG CACAGTTGGG 
ACAAGTGACA AAGAAGGGCC TTCAGATTGG GCAGAACATT TGTGTAAAGC AGCATCTAAG 
AGTGGCGAAT CAGGGGGGTC CCCAGGGGAA GCAAGTATAT TAGATGAGCT CAAGACAGAC 
TTGCAAGGGG AAGCCAGAGG GAAGGACGAA GCCCAGGGTG ACCTTGCTGA GGAGAAGGTT 
GGGAAGGAAG ACACCACAGC GGCTTCTCAG GAAGACACTG GTAAGAAAGA AGAAACAAAA 
CCTGAACCCA ATGAAGTTAG GGAGAAAGAG GAGGCCATGC TGGCCTCGGA GAAGCAGAAG 
GTTGATGAGA AAGAGACCAA TCTAGAATCT AAGGAGAAAT CGGATGTGAA TGACAAAGCC 
AAGCCTGAAC CCAAGGAGGA TGCTGGAGCA GAGGTTACTG TCAACGAGGC TGAGACTGAG 
TCTCAGGAGG AGGCTGATGT GAAGGACCAG GCCAAGCCCG AGCTTCCAGA AGTTGATGGG 
AAAGAGACCG GAAGTGACAC AAAGGAGTTG GTGGAGCCAG AGAGTCCCAC TGAAGAGCAA 
GAGCAGGGTA AGGAAAATGA ATCAGAAGAA AGGGCTGCAG TGATTCCCAG CTCCCCCGAG 
GAGTGGCCCG AAAGCCCCAC AGATGAGGGG CCCAGCCTCA GCCCAGATGG ATTGGCTCCA 
GAGTCTACAG GAGAGACCAG TCCTTCAGCC AGTGAATCTT CGCCCAGTGA GGTGCCAGGG 
AGTCCCACAG AGCCTCAACC CTCAGAGAAA AAGAAGGATC GAGCACCAGA ACGCAGAGTG 
TCGGCACCCA GCCGGCCCCG GGGGCCCCGT GCACAGAATC GGAAGGCCAT CATGGACAAA 
TTTGGCGGGG CAGCCTCTGG CCCCACTGCA CTGTTCCGGA ACACAAAGGC AGCCGGGGCA 
GCCATCGGTG GTGTCAAGAA CATGCTTTTG GAGTGGTGCC GGGCCATGAC GAGAAACTAC 
GAGCACGTGG ATATCCAAAA CTTCTCCTCC AGCTGGAGCA GCGGCATGGC CTTCTGTGCC 
CTCATCCACA AGTTTTTTCC AGAAGCCTTT GACTATGCAG AGCTGGACCC AGCAAAGCGC 
CGGCACAACT TCACCCTAGC TTTCTCGACT GCAGAGAAAC TAGCCGACTG TGCCCAGCTG 
CTGGAGGTGG ATGACATGGT GCGGCTGGCA GTGCCCGATT CCAAGTGTGT CTACACCTAC 
ATCCAGGAGC TATACCGTAG CCTTGTGCAG AAAGGGCTGG TGAAGACGAA AAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077192.1
CDS	163..1542
Translation	MEQTEGNSSEDGTTVSPTAGNLETPGSQGIAEEVAEGTVGTSDKEGPSDWAEHLCKAASKSGESGGSPGEASILDELKTDLQGEARGKDEAQGDLAEEKVGKEDTTAASQEDTGKKEETKPEPNEVREKEEAMLASEKQKVDEKETNLESKEKSDVNDKAKPEPKEDAGAEVTVNEAETESQEEADVKDQAKPELPEVDGKETGSDTKELVEPESPTEEQEQGKENESEERAAVIPSSPEEWPESPTDEGPSLSPDGLAPESTGETSPSASESSPSEVPGSPTEPQPSEKKKDRAPERRVSAPSRPRGPRAQNRKAIMDKFGGAASGPTALFRNTKAAGAAIGGVKNMLLEWCRAMTRNYEHVDIQNFSSSWSSGMAFCALIHKFFPEAFDYAELDPAKRRHNFTLAFSTAEKLADCAQLLEVDDMVRLAVPDSKCVYTYIQELYRSLVQKGLVKTKKK

Target ORF information:

RefSeq Version	NM_024230.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus smoothelin-like 1 (Smtnl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024230.2

ORF Insert Sequence:

ATGGAGCAGA CAGAAGGGAA CTCCTCTGAG GATGGGACCA CTGTCTCCCC AACTGCAGGG 
AATCTTGAGA CACCAGGAAG TCAAGGCATT GCAGAGGAGG TGGCCGAAGG CACAGTTGGG 
ACAAGTGACA AAGAAGGGCC TTCAGATTGG GCAGAACATT TGTGTAAAGC AGCATCTAAG 
AGTGGCGAAT CAGGGGGGTC CCCAGGGGAA GCAAGTATAT TAGATGAGCT CAAGACAGAC 
TTGCAAGGGG AAGCCAGAGG GAAGGACGAA GCCCAGGGTG ACCTTGCTGA GGAGAAGGTT 
GGGAAGGAAG ACACCACAGC GGCTTCTCAG GAAGACACTG GTAAGAAAGA AGAAACAAAA 
CCTGAACCCA ATGAAGTTAG GGAGAAAGAG GAGGCCATGC TGGCCTCGGA GAAGCAGAAG 
GTTGATGAGA AAGAGACCAA TCTAGAATCT AAGGAGAAAT CGGATGTGAA TGACAAAGCC 
AAGCCTGAAC CCAAGGAGGA TGCTGGAGCA GAGGTTACTG TCAACGAGGC TGAGACTGAG 
TCTCAGGAGG AGGCTGATGT GAAGGACCAG GCCAAGCCCG AGCTTCCAGA AGTTGATGGG 
AAAGAGACCG GAAGTGACAC AAAGGAGTTG GTGGAGCCAG AGAGTCCCAC TGAAGAGCAA 
GAGCAGGGTA AGGAAAATGA ATCAGAAGAA AGGGCTGCAG TGATTCCCAG CTCCCCCGAG 
GAGTGGCCCG AAAGCCCCAC AGATGAGGGG CCCAGCCTCA GCCCAGATGG ATTGGCTCCA 
GAGTCTACAG GAGAGACCAG TCCTTCAGCC AGTGAATCTT CGCCCAGTGA GGTGCCAGGG 
AGTCCCACAG AGCCTCAACC CTCAGAGAAA AAGAAGGATC GAGCACCAGA ACGCAGAGTG 
TCGGCACCCA GCCGGCCCCG GGGGCCCCGT GCACAGAATC GGAAGGCCAT CATGGACAAA 
TTTGGCGGGG CAGCCTCTGG CCCCACTGCA CTGTTCCGGA ACACAAAGGC AGCCGGGGCA 
GCCATCGGTG GTGTCAAGAA CATGCTTTTG GAGTGGTGCC GGGCCATGAC GAGAAACTAC 
GAGCACGTGG ATATCCAAAA CTTCTCCTCC AGCTGGAGCA GCGGCATGGC CTTCTGTGCC 
CTCATCCACA AGTTTTTTCC AGAAGCCTTT GACTATGCAG AGCTGGACCC AGCAAAGCGC 
CGGCACAACT TCACCCTAGC TTTCTCGACT GCAGAGAAAC TAGCCGACTG TGCCCAGCTG 
CTGGAGGTGG ATGACATGGT GCGGCTGGCA GTGCCCGATT CCAAGTGTGT CTACACCTAC 
ATCCAGGAGC TATACCGTAG CCTTGTGCAG AAAGGGCTGG TGAAGACGAA AAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smtnl1 ( XM_006500109.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500172.1 Mus musculus smoothelin-like 1 (Smtnl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16300
Clone ID Related Accession (Same CDS sequence)	XM_006500109.3 , XM_006500109.4 , NM_024230.2
Accession Version	XM_006500109.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	smoothelin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500109.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGA CAGAAGGGAA CTCCTCTGAG GATGGGACCA CTGTCTCCCC AACTGCAGGG 
AATCTTGAGA CACCAGGAAG TCAAGGCATT GCAGAGGAGG TGGCCGAAGG CACAGTTGGG 
ACAAGTGACA AAGAAGGGCC TTCAGATTGG GCAGAACATT TGTGTAAAGC AGCATCTAAG 
AGTGGCGAAT CAGGGGGGTC CCCAGGGGAA GCAAGTATAT TAGATGAGCT CAAGACAGAC 
TTGCAAGGGG AAGCCAGAGG GAAGGACGAA GCCCAGGGTG ACCTTGCTGA GGAGAAGGTT 
GGGAAGGAAG ACACCACAGC GGCTTCTCAG GAAGACACTG GTAAGAAAGA AGAAACAAAA 
CCTGAACCCA ATGAAGTTAG GGAGAAAGAG GAGGCCATGC TGGCCTCGGA GAAGCAGAAG 
GTTGATGAGA AAGAGACCAA TCTAGAATCT AAGGAGAAAT CGGATGTGAA TGACAAAGCC 
AAGCCTGAAC CCAAGGAGGA TGCTGGAGCA GAGGTTACTG TCAACGAGGC TGAGACTGAG 
TCTCAGGAGG AGGCTGATGT GAAGGACCAG GCCAAGCCCG AGCTTCCAGA AGTTGATGGG 
AAAGAGACCG GAAGTGACAC AAAGGAGTTG GTGGAGCCAG AGAGTCCCAC TGAAGAGCAA 
GAGCAGGGTA AGGAAAATGA ATCAGAAGAA AGGGCTGCAG TGATTCCCAG CTCCCCCGAG 
GAGTGGCCCG AAAGCCCCAC AGATGAGGGG CCCAGCCTCA GCCCAGATGG ATTGGCTCCA 
GAGTCTACAG GAGAGACCAG TCCTTCAGCC AGTGAATCTT CGCCCAGTGA GGTGCCAGGG 
AGTCCCACAG AGCCTCAACC CTCAGAGAAA AAGAAGGATC GAGCACCAGA ACGCAGAGTG 
TCGGCACCCA GCCGGCCCCG GGGGCCCCGT GCACAGAATC GGAAGGCCAT CATGGACAAA 
TTTGGCGGGG CAGCCTCTGG CCCCACTGCA CTGTTCCGGA ACACAAAGGC AGCCGGGGCA 
GCCATCGGTG GTGTCAAGAA CATGCTTTTG GAGTGGTGCC GGGCCATGAC GAGAAACTAC 
GAGCACGTGG ATATCCAAAA CTTCTCCTCC AGCTGGAGCA GCGGCATGGC CTTCTGTGCC 
CTCATCCACA AGTTTTTTCC AGAAGCCTTT GACTATGCAG AGCTGGACCC AGCAAAGCGC 
CGGCACAACT TCACCCTAGC TTTCTCGACT GCAGAGAAAC TAGCCGACTG TGCCCAGCTG 
CTGGAGGTGG ATGACATGGT GCGGCTGGCA GTGCCCGATT CCAAGTGTGT CTACACCTAC 
ATCCAGGAGC TATACCGTAG CCTTGTGCAG AAAGGGCTGG TGAAGACGAA AAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500172.1
CDS	723..2102
Translation	MEQTEGNSSEDGTTVSPTAGNLETPGSQGIAEEVAEGTVGTSDKEGPSDWAEHLCKAASKSGESGGSPGEASILDELKTDLQGEARGKDEAQGDLAEEKVGKEDTTAASQEDTGKKEETKPEPNEVREKEEAMLASEKQKVDEKETNLESKEKSDVNDKAKPEPKEDAGAEVTVNEAETESQEEADVKDQAKPELPEVDGKETGSDTKELVEPESPTEEQEQGKENESEERAAVIPSSPEEWPESPTDEGPSLSPDGLAPESTGETSPSASESSPSEVPGSPTEPQPSEKKKDRAPERRVSAPSRPRGPRAQNRKAIMDKFGGAASGPTALFRNTKAAGAAIGGVKNMLLEWCRAMTRNYEHVDIQNFSSSWSSGMAFCALIHKFFPEAFDYAELDPAKRRHNFTLAFSTAEKLADCAQLLEVDDMVRLAVPDSKCVYTYIQELYRSLVQKGLVKTKKK

Target ORF information:

RefSeq Version	XM_006500109.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus smoothelin-like 1 (Smtnl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500109.3

ORF Insert Sequence:

ATGGAGCAGA CAGAAGGGAA CTCCTCTGAG GATGGGACCA CTGTCTCCCC AACTGCAGGG 
AATCTTGAGA CACCAGGAAG TCAAGGCATT GCAGAGGAGG TGGCCGAAGG CACAGTTGGG 
ACAAGTGACA AAGAAGGGCC TTCAGATTGG GCAGAACATT TGTGTAAAGC AGCATCTAAG 
AGTGGCGAAT CAGGGGGGTC CCCAGGGGAA GCAAGTATAT TAGATGAGCT CAAGACAGAC 
TTGCAAGGGG AAGCCAGAGG GAAGGACGAA GCCCAGGGTG ACCTTGCTGA GGAGAAGGTT 
GGGAAGGAAG ACACCACAGC GGCTTCTCAG GAAGACACTG GTAAGAAAGA AGAAACAAAA 
CCTGAACCCA ATGAAGTTAG GGAGAAAGAG GAGGCCATGC TGGCCTCGGA GAAGCAGAAG 
GTTGATGAGA AAGAGACCAA TCTAGAATCT AAGGAGAAAT CGGATGTGAA TGACAAAGCC 
AAGCCTGAAC CCAAGGAGGA TGCTGGAGCA GAGGTTACTG TCAACGAGGC TGAGACTGAG 
TCTCAGGAGG AGGCTGATGT GAAGGACCAG GCCAAGCCCG AGCTTCCAGA AGTTGATGGG 
AAAGAGACCG GAAGTGACAC AAAGGAGTTG GTGGAGCCAG AGAGTCCCAC TGAAGAGCAA 
GAGCAGGGTA AGGAAAATGA ATCAGAAGAA AGGGCTGCAG TGATTCCCAG CTCCCCCGAG 
GAGTGGCCCG AAAGCCCCAC AGATGAGGGG CCCAGCCTCA GCCCAGATGG ATTGGCTCCA 
GAGTCTACAG GAGAGACCAG TCCTTCAGCC AGTGAATCTT CGCCCAGTGA GGTGCCAGGG 
AGTCCCACAG AGCCTCAACC CTCAGAGAAA AAGAAGGATC GAGCACCAGA ACGCAGAGTG 
TCGGCACCCA GCCGGCCCCG GGGGCCCCGT GCACAGAATC GGAAGGCCAT CATGGACAAA 
TTTGGCGGGG CAGCCTCTGG CCCCACTGCA CTGTTCCGGA ACACAAAGGC AGCCGGGGCA 
GCCATCGGTG GTGTCAAGAA CATGCTTTTG GAGTGGTGCC GGGCCATGAC GAGAAACTAC 
GAGCACGTGG ATATCCAAAA CTTCTCCTCC AGCTGGAGCA GCGGCATGGC CTTCTGTGCC 
CTCATCCACA AGTTTTTTCC AGAAGCCTTT GACTATGCAG AGCTGGACCC AGCAAAGCGC 
CGGCACAACT TCACCCTAGC TTTCTCGACT GCAGAGAAAC TAGCCGACTG TGCCCAGCTG 
CTGGAGGTGG ATGACATGGT GCGGCTGGCA GTGCCCGATT CCAAGTGTGT CTACACCTAC 
ATCCAGGAGC TATACCGTAG CCTTGTGCAG AAAGGGCTGG TGAAGACGAA AAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smtnl1 ( XM_006500109.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500172.1 Mus musculus smoothelin-like 1 (Smtnl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16300
Clone ID Related Accession (Same CDS sequence)	XM_006500109.3 , XM_006500109.4 , NM_024230.2
Accession Version	XM_006500109.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	smoothelin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500109.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGA CAGAAGGGAA CTCCTCTGAG GATGGGACCA CTGTCTCCCC AACTGCAGGG 
AATCTTGAGA CACCAGGAAG TCAAGGCATT GCAGAGGAGG TGGCCGAAGG CACAGTTGGG 
ACAAGTGACA AAGAAGGGCC TTCAGATTGG GCAGAACATT TGTGTAAAGC AGCATCTAAG 
AGTGGCGAAT CAGGGGGGTC CCCAGGGGAA GCAAGTATAT TAGATGAGCT CAAGACAGAC 
TTGCAAGGGG AAGCCAGAGG GAAGGACGAA GCCCAGGGTG ACCTTGCTGA GGAGAAGGTT 
GGGAAGGAAG ACACCACAGC GGCTTCTCAG GAAGACACTG GTAAGAAAGA AGAAACAAAA 
CCTGAACCCA ATGAAGTTAG GGAGAAAGAG GAGGCCATGC TGGCCTCGGA GAAGCAGAAG 
GTTGATGAGA AAGAGACCAA TCTAGAATCT AAGGAGAAAT CGGATGTGAA TGACAAAGCC 
AAGCCTGAAC CCAAGGAGGA TGCTGGAGCA GAGGTTACTG TCAACGAGGC TGAGACTGAG 
TCTCAGGAGG AGGCTGATGT GAAGGACCAG GCCAAGCCCG AGCTTCCAGA AGTTGATGGG 
AAAGAGACCG GAAGTGACAC AAAGGAGTTG GTGGAGCCAG AGAGTCCCAC TGAAGAGCAA 
GAGCAGGGTA AGGAAAATGA ATCAGAAGAA AGGGCTGCAG TGATTCCCAG CTCCCCCGAG 
GAGTGGCCCG AAAGCCCCAC AGATGAGGGG CCCAGCCTCA GCCCAGATGG ATTGGCTCCA 
GAGTCTACAG GAGAGACCAG TCCTTCAGCC AGTGAATCTT CGCCCAGTGA GGTGCCAGGG 
AGTCCCACAG AGCCTCAACC CTCAGAGAAA AAGAAGGATC GAGCACCAGA ACGCAGAGTG 
TCGGCACCCA GCCGGCCCCG GGGGCCCCGT GCACAGAATC GGAAGGCCAT CATGGACAAA 
TTTGGCGGGG CAGCCTCTGG CCCCACTGCA CTGTTCCGGA ACACAAAGGC AGCCGGGGCA 
GCCATCGGTG GTGTCAAGAA CATGCTTTTG GAGTGGTGCC GGGCCATGAC GAGAAACTAC 
GAGCACGTGG ATATCCAAAA CTTCTCCTCC AGCTGGAGCA GCGGCATGGC CTTCTGTGCC 
CTCATCCACA AGTTTTTTCC AGAAGCCTTT GACTATGCAG AGCTGGACCC AGCAAAGCGC 
CGGCACAACT TCACCCTAGC TTTCTCGACT GCAGAGAAAC TAGCCGACTG TGCCCAGCTG 
CTGGAGGTGG ATGACATGGT GCGGCTGGCA GTGCCCGATT CCAAGTGTGT CTACACCTAC 
ATCCAGGAGC TATACCGTAG CCTTGTGCAG AAAGGGCTGG TGAAGACGAA AAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500172.1
CDS	636..2015
Translation	MEQTEGNSSEDGTTVSPTAGNLETPGSQGIAEEVAEGTVGTSDKEGPSDWAEHLCKAASKSGESGGSPGEASILDELKTDLQGEARGKDEAQGDLAEEKVGKEDTTAASQEDTGKKEETKPEPNEVREKEEAMLASEKQKVDEKETNLESKEKSDVNDKAKPEPKEDAGAEVTVNEAETESQEEADVKDQAKPELPEVDGKETGSDTKELVEPESPTEEQEQGKENESEERAAVIPSSPEEWPESPTDEGPSLSPDGLAPESTGETSPSASESSPSEVPGSPTEPQPSEKKKDRAPERRVSAPSRPRGPRAQNRKAIMDKFGGAASGPTALFRNTKAAGAAIGGVKNMLLEWCRAMTRNYEHVDIQNFSSSWSSGMAFCALIHKFFPEAFDYAELDPAKRRHNFTLAFSTAEKLADCAQLLEVDDMVRLAVPDSKCVYTYIQELYRSLVQKGLVKTKKK

Target ORF information:

RefSeq Version	XM_006500109.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus smoothelin-like 1 (Smtnl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500109.4

ORF Insert Sequence:

ATGGAGCAGA CAGAAGGGAA CTCCTCTGAG GATGGGACCA CTGTCTCCCC AACTGCAGGG 
AATCTTGAGA CACCAGGAAG TCAAGGCATT GCAGAGGAGG TGGCCGAAGG CACAGTTGGG 
ACAAGTGACA AAGAAGGGCC TTCAGATTGG GCAGAACATT TGTGTAAAGC AGCATCTAAG 
AGTGGCGAAT CAGGGGGGTC CCCAGGGGAA GCAAGTATAT TAGATGAGCT CAAGACAGAC 
TTGCAAGGGG AAGCCAGAGG GAAGGACGAA GCCCAGGGTG ACCTTGCTGA GGAGAAGGTT 
GGGAAGGAAG ACACCACAGC GGCTTCTCAG GAAGACACTG GTAAGAAAGA AGAAACAAAA 
CCTGAACCCA ATGAAGTTAG GGAGAAAGAG GAGGCCATGC TGGCCTCGGA GAAGCAGAAG 
GTTGATGAGA AAGAGACCAA TCTAGAATCT AAGGAGAAAT CGGATGTGAA TGACAAAGCC 
AAGCCTGAAC CCAAGGAGGA TGCTGGAGCA GAGGTTACTG TCAACGAGGC TGAGACTGAG 
TCTCAGGAGG AGGCTGATGT GAAGGACCAG GCCAAGCCCG AGCTTCCAGA AGTTGATGGG 
AAAGAGACCG GAAGTGACAC AAAGGAGTTG GTGGAGCCAG AGAGTCCCAC TGAAGAGCAA 
GAGCAGGGTA AGGAAAATGA ATCAGAAGAA AGGGCTGCAG TGATTCCCAG CTCCCCCGAG 
GAGTGGCCCG AAAGCCCCAC AGATGAGGGG CCCAGCCTCA GCCCAGATGG ATTGGCTCCA 
GAGTCTACAG GAGAGACCAG TCCTTCAGCC AGTGAATCTT CGCCCAGTGA GGTGCCAGGG 
AGTCCCACAG AGCCTCAACC CTCAGAGAAA AAGAAGGATC GAGCACCAGA ACGCAGAGTG 
TCGGCACCCA GCCGGCCCCG GGGGCCCCGT GCACAGAATC GGAAGGCCAT CATGGACAAA 
TTTGGCGGGG CAGCCTCTGG CCCCACTGCA CTGTTCCGGA ACACAAAGGC AGCCGGGGCA 
GCCATCGGTG GTGTCAAGAA CATGCTTTTG GAGTGGTGCC GGGCCATGAC GAGAAACTAC 
GAGCACGTGG ATATCCAAAA CTTCTCCTCC AGCTGGAGCA GCGGCATGGC CTTCTGTGCC 
CTCATCCACA AGTTTTTTCC AGAAGCCTTT GACTATGCAG AGCTGGACCC AGCAAAGCGC 
CGGCACAACT TCACCCTAGC TTTCTCGACT GCAGAGAAAC TAGCCGACTG TGCCCAGCTG 
CTGGAGGTGG ATGACATGGT GCGGCTGGCA GTGCCCGATT CCAAGTGTGT CTACACCTAC 
ATCCAGGAGC TATACCGTAG CCTTGTGCAG AAAGGGCTGG TGAAGACGAA AAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pregnancy and Smoothelin-like Protein 1 (SMTNL1) Deletion Promote the Switching of Skeletal Muscle to a Glycolytic Phenotype in Human and Mice.
The Journal of biological chemistry290(29)17985-98(2015 Jul)
Lontay B,Bodoor K,Sipos A,Weitzel DH,Loiselle D,Safi R,Zheng D,Devente J,Hickner RC,McDonnell DP,Ribar T,Haystead TA

Two domains of the smoothelin-like 1 protein bind apo- and calcium-calmodulin independently.
Biochimica et biophysica acta1844(9)1580-90(2014 Sep)
Ulke-Lem?e A,Ishida H,Chappellaz M,Vogel HJ,MacDonald JA

Intrinsically disordered N-terminus of calponin homology-associated smooth muscle protein (CHASM) interacts with the calponin homology domain to enable tropomyosin binding.
Biochemistry51(13)2694-705(2012 Apr)
MacDonald JA,Ishida H,Butler EI,Ulke-Lem?e A,Chappellaz M,Tulk SE,Chik JK,Vogel HJ

Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression.
Developmental biology359(2)303-20(2011 Nov)
Richard AF,Demignon J,Sakakibara I,Pujol J,Favier M,Strochlic L,Le Grand F,Sgarioto N,Guernec A,Schmitt A,Cagnard N,Huang R,Legay C,Guillet-Deniau I,Maire P

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Smtnl1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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