Trpm4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trpm4
Entrez Gene ID	68667
Full Name	transient receptor potential cation channel, subfamily M, member 4
Synonyms	1110030C19Rik,AW047689,LTRPC4,LTrpC-4,TRPM4B
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 4 Names transient receptor potential cation channel subfamily M member 4 calcium-activated non-selective cation channel 1 long transient receptor potential channel 4 transient receptor potential ion channel melastatin subgroup member 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_175130.4	NP_780339.2	transient receptor potential cation channel subfamily M member 4
XM_006541146.3	XP_006541209.1	transient receptor potential cation channel subfamily M member 4 isoform X1
XM_006541148.3	XP_006541211.1	transient receptor potential cation channel subfamily M member 4 isoform X2
XM_030242952.1	XP_030098812.1	transient receptor potential cation channel subfamily M member 4 isoform X2
XM_006541146.4	XP_006541209.1	transient receptor potential cation channel subfamily M member 4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRPM4	NP_060106.2
Canis lupus familiaris (dog)	TRPM4	XP_541500.3
Mus musculus (house mouse)	Trpm4	NP_780339.2
Danio rerio (zebrafish)	LOC100334234	XP_003199613.2
Danio rerio (zebrafish)	LOC100333667	XP_002663943.3
Pan troglodytes (chimpanzee)	TRPM4	XP_512811.2
Bos taurus (cattle)	TRPM4	XP_002695251.2
Rattus norvegicus (Norway rat)	Trpm4	NP_001129701.1
Danio rerio (zebrafish)	trpm4c	XP_001339981.5
	LOC719120	XP_001112814.2
Danio rerio (zebrafish)	trpm4a	XP_003198181.2
Xenopus tropicalis (tropical clawed frog)	trpm5	XP_004916436.1

Gene Ontology
Bibliography

Related articles in PubMed

TRPM4 and TRPM5 are both required for normal signaling in taste receptor cells.
Dutta Banik D, Martin LE, Freichel M, Torregrossa AM, Medler KF
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)

Structures of the calcium-activated, non-selective cation channel TRPM4.
Guo J, She J, Zeng W, Chen Q, Bai XC, Jiang Y
Nature552(7684)205-209(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Disrupting the Trpm4 gene in mice specifically eliminates NMDAR-dependent LTP.
Title: TRPM4-dependent post-synaptic depolarization is essential for the induction of NMDA receptor-dependent LTP in CA1 hippocampal neurons.

The PKC-dependent effect of GLP-1 on membrane potential and electrical activity was mediated by activation of Na(+)-permeable TRPM4 and TRPM5 channels by mobilization of intracellular Ca(2+) from thapsigargin-sensitive Ca(2+) stores
Title: GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation.

The function of TRPM4 in renal primary cilia is not yet known, but it is likely to influence the apical Ca(2) dynamics of the cell
Title: A TRPM4-dependent current in murine renal primary cilia.

Deletion of the Trpm4 gene in mice improved survival and significantly enhanced beta-adrenergic cardiac reserve after inducing ischaemic heart failure.
Title: Enhanced ?-adrenergic cardiac reserve in Trpm4?/? mice with ischaemic heart failure.

observations are consistent with a model in which TRPM4 is a regulator of calcium homeostasis in cardiomyocytes after AngII stimulation
Title: The Ca(2+)-activated cation channel TRPM4 is a negative regulator of angiotensin II-induced cardiac hypertrophy.

The following Trpm4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trpm4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu89617	XM_030242952.1 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu15929	NM_175130.4 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OMu38998	XM_006541146.4 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu39000	XM_006541148.3 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu38998	XM_006541146.3	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trpm4 ( XM_030242952.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098812.1 Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu89617
Clone ID Related Accession (Same CDS sequence)	XM_030242952.1
Accession Version	XM_030242952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGAA AGGAGTTGCT GACGGTCTAT TCATCAGAAG ACGGCTCCGA GGAGTTTGAG 
ACTATCGTTT TGAGGGCTCT TGTGAAAGCC TGTGGGAGCT CTGAGGCCTC AGCCTACCTG 
GATGAGCTGC GTTTGGCTGT GGCTTGGAAC CGCGTGGACA TCGCCCAAAG TGAACTTTTC 
CGTGGGGACA TCCAATGGCG GTCCTTCCAC CTGGAGGCAT CTCTCATGGA TGCTCTGCTG 
AATGACCGGC CTGAATTTGT GCGCCTGCTC ATCTCCCATG GCCTTAGCCT GGGACACTTC 
CTGACCCCCG TGCGTCTGGC CCAGCTGTAC AGCGCGGTGT CCCCTAACTC ACTGATCCGA 
AATCTTCTGG ACCAGGCGTC CCATGCTAGT AGCAGCAAAT CCCCTCCTGT AAATGGAACT 
GTAGAGCTCC GGCCTCCCAA TGTGGGGCAA GTGCTGAGGA CTCTGTTGGG AGAAACGTGT 
GCACCACGGT ACCCTGCCAG GAACACCCGA GACTCCTATC TGGGCCAGGA CCACAGAGAG 
AATGACTCTC TGCTTATGGA CTGGGCCAAC AAGCAACCAT CAACGGATGC AAGCTTTGAA 
CAAGCCCCCT GGAGTGACCT GCTTATTTGG GCTCTGTTGC TGAACCGGGC CCAGATGGCT 
ATCTATTTCT GGGAGAAGGG CTCCAACTCA GTGGCCTCTG CTCTTGGGGC TTGTCTTTTA 
CTCCGAGTGA TGGCTCGCCT AGAGTCGGAG GCTGAGGAGG CCGCGCGGCG GAAGGATCTT 
GCTGCCACGT TTGAAAGCAT GAGCGTGGAC CTCTTTGGAG AGTGTTACCA CAACAGTGAA 
GAGCGAGCAG CCCGCCTTCT TCTGCGCCGC TGTCCCCTCT GGGGAGAGGC CACCTGCCTC 
CAGCTTGCCA TGCAGGCTGA TGCCCGAGCG TTCTTTGCCC AGGATGGAGT CCAGTCTCTT 
CTGACACAGA AGTGGTGGGG GGAGATGGAC AGCACAACTC CCATCTGGGC CCTGCTTCTC 
GCCTTCTTCT GCCCGCCTCT CATCTACACC AACCTTATCG TCTTCAGGAA GTCAGAGGAG 
GAGCCCACAC AGAAGGATCT TGACTTTGAT ATGGACAGCA GCATCAATGG AGCAGGTCCT 
CCTGGGACTG TAGAACCCTC TGCAAAGGTG GCCCTTGAGA GGCGGCAGCG GCGACGGCCA 
GGACGTGCCC TCTGCTGTGG CAAGTTCTCC AAGCGCTGGT CGGACTTCTG GGGTGCCCCA 
GTGACTGCCT TCCTGGGTAA TGTGGTCAGT TACCTGCTGT TCCTGCTGCT GTTTGCCCAC 
GTGTTGCTGG TGGATTTCCA GCCCACAAAG CCAAGTGTCT CCGAGCTGCT GCTGTACTTC 
TGGGCCTTCA CGTTGCTGTG CGAGGAGCTG CGCCAGGGCC TGGGTGGTGG CTGGGGTAGC 
CTGGCCAGTG GGGGACGTGG TCCGGACAGA GCTCCCCTGC GCCATCGGCT GCACCTCTAC 
CTCTCAGATA CCTGGAACCA ATGTGACCTG CTGGCACTCA CCTGCTTCCT GCTGGGCGTA 
GGCTGCCGGC TGACCCCTGG GCTCTTTGAC TTGGGACGCA CCGTCCTCTG CCTTGACTTC 
ATGATCTTCA CACTGCGCCT GCTGCACATC TTCACGGTGA ACAAGCAGCT GGGGCCCAAG 
ATTGTCATAG TGAGCAAAAT GATGAAGGAT GTATTCTTCT TCCTCTTCTT CCTCTGCGTG 
TGGCTTGTAG CTTATGGGGT GGCCACAGAG GGGATCCTGA GGCCCCAGGA CCGCAGTCTA 
CCGAGTATCC TGCGCAGGGT CTTCTACCGA CCGTATCTGC AGATCTTCGG ACAAATTCCC 
CAGGAGGAAA TGGACGTGGC CCTCATGATT CCAGGTAACT GCTCAATGGA GCGGGGCTCC 
TGGGCTCATC CGGAGGGGCC CGTCGCAGGC TCCTGTGTGT CCCAGTATGC CAACTGGCTG 
GTGGTGTTGC TCCTTATCGT CTTCTTGCTG GTGGCCAATA TCCTGTTGCT CAACCTGCTC 
ATCGCCATGT TCAGCTACAC CTTCAGCAAA GTGCACGGCA ACAGCGACCT CTACTGGAAG 
GCACAGCGCT ACAGCCTCAT CCGGGAATTC CATTCGAGGC CTGCCCTGGC CCCACCCCTC 
ATCATCATCT CTCATGTGCG CCTCCTCATC AAGTGGCTGC GCAGGTGTCG CAGGTGTCGC 
AGGGCTAACT TGCCCGCCTC CCCAGTCTTC GAGCACTTCC GTGTCTGTCT CTCTAAGGAA 
GCAGAGCGGA AGCTGCTGAC TTGGGAGTCT GTGCACAAAG AGAACTTCCT GTTGGCACAA 
GCTCGGGACA AGCGAGACAG TGACTCGGAG CGTTTGAAAC GCACGTCTCA GAAGGTGGAC 
ACTGCACTGA AGCAGCTGGG ACAGATCAGA GAGTACGATC GGCGTCTGAG GGGGCTGGAG 
AGAGAGGTCC AACACTGTTC TCGAGTCCTG ACTTGGATGG CTGAGGCCCT TAGCCACTCT 
GCCTTGCTGC CTCCAGGGGC CCCACCCCCT CCGAGCCCCA CTGGTTCCAA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098812.1
CDS	1060..3636
Translation	MTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSPPVNGTVELRPPNVGQVLRTLLGETCAPRYPARNTRDSYLGQDHRENDSLLMDWANKQPSTDASFEQAPWSDLLIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLESEAEEAARRKDLAATFESMSVDLFGECYHNSEERAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTTPIWALLLAFFCPPLIYTNLIVFRKSEEEPTQKDLDFDMDSSINGAGPPGTVEPSAKVALERRQRRRPGRALCCGKFSKRWSDFWGAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPSVSELLLYFWAFTLLCEELRQGLGGGWGSLASGGRGPDRAPLRHRLHLYLSDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNKQLGPKIVIVSKMMKDVFFFLFFLCVWLVAYGVATEGILRPQDRSLPSILRRVFYRPYLQIFGQIPQEEMDVALMIPGNCSMERGSWAHPEGPVAGSCVSQYANWLVVLLLIVFLLVANILLLNLLIAMFSYTFSKVHGNSDLYWKAQRYSLIREFHSRPALAPPLIIISHVRLLIKWLRRCRRCRRANLPASPVFEHFRVCLSKEAERKLLTWESVHKENFLLAQARDKRDSDSERLKRTSQKVDTALKQLGQIREYDRRLRGLEREVQHCSRVLTWMAEALSHSALLPPGAPPPPSPTGSKD

Target ORF information:

RefSeq Version	XM_030242952.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242952.1

ORF Insert Sequence:

ATGACCCGAA AGGAGTTGCT GACGGTCTAT TCATCAGAAG ACGGCTCCGA GGAGTTTGAG 
ACTATCGTTT TGAGGGCTCT TGTGAAAGCC TGTGGGAGCT CTGAGGCCTC AGCCTACCTG 
GATGAGCTGC GTTTGGCTGT GGCTTGGAAC CGCGTGGACA TCGCCCAAAG TGAACTTTTC 
CGTGGGGACA TCCAATGGCG GTCCTTCCAC CTGGAGGCAT CTCTCATGGA TGCTCTGCTG 
AATGACCGGC CTGAATTTGT GCGCCTGCTC ATCTCCCATG GCCTTAGCCT GGGACACTTC 
CTGACCCCCG TGCGTCTGGC CCAGCTGTAC AGCGCGGTGT CCCCTAACTC ACTGATCCGA 
AATCTTCTGG ACCAGGCGTC CCATGCTAGT AGCAGCAAAT CCCCTCCTGT AAATGGAACT 
GTAGAGCTCC GGCCTCCCAA TGTGGGGCAA GTGCTGAGGA CTCTGTTGGG AGAAACGTGT 
GCACCACGGT ACCCTGCCAG GAACACCCGA GACTCCTATC TGGGCCAGGA CCACAGAGAG 
AATGACTCTC TGCTTATGGA CTGGGCCAAC AAGCAACCAT CAACGGATGC AAGCTTTGAA 
CAAGCCCCCT GGAGTGACCT GCTTATTTGG GCTCTGTTGC TGAACCGGGC CCAGATGGCT 
ATCTATTTCT GGGAGAAGGG CTCCAACTCA GTGGCCTCTG CTCTTGGGGC TTGTCTTTTA 
CTCCGAGTGA TGGCTCGCCT AGAGTCGGAG GCTGAGGAGG CCGCGCGGCG GAAGGATCTT 
GCTGCCACGT TTGAAAGCAT GAGCGTGGAC CTCTTTGGAG AGTGTTACCA CAACAGTGAA 
GAGCGAGCAG CCCGCCTTCT TCTGCGCCGC TGTCCCCTCT GGGGAGAGGC CACCTGCCTC 
CAGCTTGCCA TGCAGGCTGA TGCCCGAGCG TTCTTTGCCC AGGATGGAGT CCAGTCTCTT 
CTGACACAGA AGTGGTGGGG GGAGATGGAC AGCACAACTC CCATCTGGGC CCTGCTTCTC 
GCCTTCTTCT GCCCGCCTCT CATCTACACC AACCTTATCG TCTTCAGGAA GTCAGAGGAG 
GAGCCCACAC AGAAGGATCT TGACTTTGAT ATGGACAGCA GCATCAATGG AGCAGGTCCT 
CCTGGGACTG TAGAACCCTC TGCAAAGGTG GCCCTTGAGA GGCGGCAGCG GCGACGGCCA 
GGACGTGCCC TCTGCTGTGG CAAGTTCTCC AAGCGCTGGT CGGACTTCTG GGGTGCCCCA 
GTGACTGCCT TCCTGGGTAA TGTGGTCAGT TACCTGCTGT TCCTGCTGCT GTTTGCCCAC 
GTGTTGCTGG TGGATTTCCA GCCCACAAAG CCAAGTGTCT CCGAGCTGCT GCTGTACTTC 
TGGGCCTTCA CGTTGCTGTG CGAGGAGCTG CGCCAGGGCC TGGGTGGTGG CTGGGGTAGC 
CTGGCCAGTG GGGGACGTGG TCCGGACAGA GCTCCCCTGC GCCATCGGCT GCACCTCTAC 
CTCTCAGATA CCTGGAACCA ATGTGACCTG CTGGCACTCA CCTGCTTCCT GCTGGGCGTA 
GGCTGCCGGC TGACCCCTGG GCTCTTTGAC TTGGGACGCA CCGTCCTCTG CCTTGACTTC 
ATGATCTTCA CACTGCGCCT GCTGCACATC TTCACGGTGA ACAAGCAGCT GGGGCCCAAG 
ATTGTCATAG TGAGCAAAAT GATGAAGGAT GTATTCTTCT TCCTCTTCTT CCTCTGCGTG 
TGGCTTGTAG CTTATGGGGT GGCCACAGAG GGGATCCTGA GGCCCCAGGA CCGCAGTCTA 
CCGAGTATCC TGCGCAGGGT CTTCTACCGA CCGTATCTGC AGATCTTCGG ACAAATTCCC 
CAGGAGGAAA TGGACGTGGC CCTCATGATT CCAGGTAACT GCTCAATGGA GCGGGGCTCC 
TGGGCTCATC CGGAGGGGCC CGTCGCAGGC TCCTGTGTGT CCCAGTATGC CAACTGGCTG 
GTGGTGTTGC TCCTTATCGT CTTCTTGCTG GTGGCCAATA TCCTGTTGCT CAACCTGCTC 
ATCGCCATGT TCAGCTACAC CTTCAGCAAA GTGCACGGCA ACAGCGACCT CTACTGGAAG 
GCACAGCGCT ACAGCCTCAT CCGGGAATTC CATTCGAGGC CTGCCCTGGC CCCACCCCTC 
ATCATCATCT CTCATGTGCG CCTCCTCATC AAGTGGCTGC GCAGGTGTCG CAGGTGTCGC 
AGGGCTAACT TGCCCGCCTC CCCAGTCTTC GAGCACTTCC GTGTCTGTCT CTCTAAGGAA 
GCAGAGCGGA AGCTGCTGAC TTGGGAGTCT GTGCACAAAG AGAACTTCCT GTTGGCACAA 
GCTCGGGACA AGCGAGACAG TGACTCGGAG CGTTTGAAAC GCACGTCTCA GAAGGTGGAC 
ACTGCACTGA AGCAGCTGGG ACAGATCAGA GAGTACGATC GGCGTCTGAG GGGGCTGGAG 
AGAGAGGTCC AACACTGTTC TCGAGTCCTG ACTTGGATGG CTGAGGCCCT TAGCCACTCT 
GCCTTGCTGC CTCCAGGGGC CCCACCCCCT CCGAGCCCCA CTGGTTCCAA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm4 ( NM_175130.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780339.2 Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), mRNA.

Price & Availability↑

CloneID	OMu15929
Clone ID Related Accession (Same CDS sequence)	NM_175130.4
Accession Version	NM_175130.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY097409.1, BY335443.1, AC150897.2, BY304579.1, BB840481.1, AK044094.1 and BC058632.1. On Oct 3, 2007 this sequence version replaced NM_175130.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096475.1, AJ575814.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGGGC CGGAGAAGGA GCAGAGCTGG ATCCCTAAGA TCTTCAGGAA GAAGGTGTGC 
ACGACGTTCA TAGTGGACCT CTCCGATGAT GCGGGAGGGA CCTTGTGCCA GTGTGGGCAA 
CCTCGGGATG CCCACCCTTC TGTGGCTGTG GAGGATGCCT TTGGGGCAGC CGTAGTTACC 
GAGTGGAACA GTGATGAGCA CACCACGGAG AAGCCCACAG ATGCCTATGG GGACCTGGAC 
TTCACGTACT CTGGCCGAAA GCACAGCAAC TTTCTCCGGC TGTCTGACCG CACGGATCCG 
GCCACTGTGT ACAGTCTCGT TACCCGCTCT TGGGGCTTTC GTGCCCCAAA CTTGGTGGTG 
TCGGTGCTGG GGGGGTCGGG GGGCCCCGTG CTCCAGACCT GGCTGCAGGA CCTTCTGCGT 
CGCGGGCTGG TGAGAGCCGC CCAGAGCACA GGGGCCTGGA TCGTCACTGG GGGACTGCAC 
ACAGGCATTG GCCGGCACGT GGGTGTGGCT GTAAGGGACC ACCAGACAGC CAGCACTGGG 
AGCAGCAAGG TGGTGGCCAT GGGTGTGGCC CCCTGGGGAG TGGTCCGGAA CAGAGACATG 
CTGATCAACC CCAAGGGCTC ATTCCCCGCA AGGTACCGGT GGCGTGGTGA CCCTGAGGAT 
GGTGTTGAGT TCCCCCTGGA CTATAACTAT TCTGCTTTCT TCTTGGTGGA TGATGGCACC 
TATGGCCGCC TGGGTGGTGA GAACCGCTTC CGCCTTCGGT TTGAGTCCTA TGTGGCTCAG 
CAGAAGACCG GAGTGGGAGG GACTGGAATT GACATCCCAG TGCTCCTTCT TCTCATCGAT 
GGAGATGAGA AGATGTTAAA GCGGATAGAG GATGCCACCC AAGCTCAGCT CCCCTGCCTC 
TTGGTGGCGG GCTCTGGGGG TGCTGCAGAC TGTCTGGTGG AGACCCTGGA AGATACTCTG 
GCCCCAGGGA GTGGGGGACT TAGGCGAGGC GAAGCCCGGG ATCGGATTAG GCGATACTTC 
CCTAAAGGAG ACCCTGAGGT TCTTCAGGCC CAGGTAGAGA GGATCATGAC CCGAAAGGAG 
TTGCTGACGG TCTATTCATC AGAAGACGGC TCCGAGGAGT TTGAGACTAT CGTTTTGAGG 
GCTCTTGTGA AAGCCTGTGG GAGCTCTGAG GCCTCAGCCT ACCTGGATGA GCTGCGTTTG 
GCTGTGGCTT GGAACCGCGT GGACATCGCC CAAAGTGAAC TTTTCCGTGG GGACATCCAA 
TGGCGGTCCT TCCACCTGGA GGCATCTCTC ATGGATGCTC TGCTGAATGA CCGGCCTGAA 
TTTGTGCGCC TGCTCATCTC CCATGGCCTT AGCCTGGGAC ACTTCCTGAC CCCCGTGCGT 
CTGGCCCAGC TGTACAGCGC GGTGTCCCCT AACTCACTGA TCCGAAATCT TCTGGACCAG 
GCGTCCCATG CTAGTAGCAG CAAATCCCCT CCTGTAAATG GAACTGTAGA GCTCCGGCCT 
CCCAATGTGG GGCAAGTGCT GAGGACTCTG TTGGGAGAAA CGTGTGCACC ACGGTACCCT 
GCCAGGAACA CCCGAGACTC CTATCTGGGC CAGGACCACA GAGAGAATGA CTCTCTGCTT 
ATGGACTGGG CCAACAAGCA ACCATCAACG GATGCAAGCT TTGAACAAGC CCCCTGGAGT 
GACCTGCTTA TTTGGGCTCT GTTGCTGAAC CGGGCCCAGA TGGCTATCTA TTTCTGGGAG 
AAGGGCTCCA ACTCAGTGGC CTCTGCTCTT GGGGCTTGTC TTTTACTCCG AGTGATGGCT 
CGCCTAGAGT CGGAGGCTGA GGAGGCCGCG CGGCGGAAGG ATCTTGCTGC CACGTTTGAA 
AGCATGAGCG TGGACCTCTT TGGAGAGTGT TACCACAACA GTGAAGAGCG AGCAGCCCGC 
CTTCTTCTGC GCCGCTGTCC CCTCTGGGGA GAGGCCACCT GCCTCCAGCT TGCCATGCAG 
GCTGATGCCC GAGCGTTCTT TGCCCAGGAT GGAGTCCAGT CTCTTCTGAC ACAGAAGTGG 
TGGGGGGAGA TGGACAGCAC AACTCCCATC TGGGCCCTGC TTCTCGCCTT CTTCTGCCCG 
CCTCTCATCT ACACCAACCT TATCGTCTTC AGGAAGTCAG AGGAGGAGCC CACACAGAAG 
GATCTTGACT TTGATATGGA CAGCAGCATC AATGGAGCAG GTCCTCCTGG GACTGTAGAA 
CCCTCTGCAA AGGTGGCCCT TGAGAGGCGG CAGCGGCGAC GGCCAGGACG TGCCCTCTGC 
TGTGGCAAGT TCTCCAAGCG CTGGTCGGAC TTCTGGGGTG CCCCAGTGAC TGCCTTCCTG 
GGTAATGTGG TCAGTTACCT GCTGTTCCTG CTGCTGTTTG CCCACGTGTT GCTGGTGGAT 
TTCCAGCCCA CAAAGCCAAG TGTCTCCGAG CTGCTGCTGT ACTTCTGGGC CTTCACGTTG 
CTGTGCGAGG AGCTGCGCCA GGGCCTGGGT GGTGGCTGGG GTAGCCTGGC CAGTGGGGGA 
CGTGGTCCGG ACAGAGCTCC CCTGCGCCAT CGGCTGCACC TCTACCTCTC AGATACCTGG 
AACCAATGTG ACCTGCTGGC ACTCACCTGC TTCCTGCTGG GCGTAGGCTG CCGGCTGACC 
CCTGGGCTCT TTGACTTGGG ACGCACCGTC CTCTGCCTTG ACTTCATGAT CTTCACACTG 
CGCCTGCTGC ACATCTTCAC GGTGAACAAG CAGCTGGGGC CCAAGATTGT CATAGTGAGC 
AAAATGATGA AGGATGTATT CTTCTTCCTC TTCTTCCTCT GCGTGTGGCT TGTAGCTTAT 
GGGGTGGCCA CAGAGGGGAT CCTGAGGCCC CAGGACCGCA GTCTACCGAG TATCCTGCGC 
AGGGTCTTCT ACCGACCGTA TCTGCAGATC TTCGGACAAA TTCCCCAGGA GGAAATGGAC 
GTGGCCCTCA TGATTCCAGG TAACTGCTCA ATGGAGCGGG GCTCCTGGGC TCATCCGGAG 
GGGCCCGTCG CAGGCTCCTG TGTGTCCCAG TATGCCAACT GGCTGGTGGT GTTGCTCCTT 
ATCGTCTTCT TGCTGGTGGC CAATATCCTG TTGCTCAACC TGCTCATCGC CATGTTCAGC 
TACACCTTCA GCAAAGTGCA CGGCAACAGC GACCTCTACT GGAAGGCACA GCGCTACAGC 
CTCATCCGGG AATTCCATTC GAGGCCTGCC CTGGCCCCAC CCCTCATCAT CATCTCTCAT 
GTGCGCCTCC TCATCAAGTG GCTGCGCAGG TGTCGCAGGT GTCGCAGGGC TAACTTGCCC 
GCCTCCCCAG TCTTCGAGCA CTTCCGTGTC TGTCTCTCTA AGGAAGCAGA GCGGAAGCTG 
CTGACTTGGG AGTCTGTGCA CAAAGAGAAC TTCCTGTTGG CACAAGCTCG GGACAAGCGA 
GACAGTGACT CGGAGCGTTT GAAACGCACG TCTCAGAAGG TGGACACTGC ACTGAAGCAG 
CTGGGACAGA TCAGAGAGTA CGATCGGCGT CTGAGGGGGC TGGAGAGAGA GGTCCAACAC 
TGTTCTCGAG TCCTGACTTG GATGGCTGAG GCCCTTAGCC ACTCTGCCTT GCTGCCTCCA 
GGGGCCCCAC CCCCTCCGAG CCCCACTGGT TCCAAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780339.2
CDS	71..3712
Translation	MVGPEKEQSWIPKIFRKKVCTTFIVDLSDDAGGTLCQCGQPRDAHPSVAVEDAFGAAVVTEWNSDEHTTEKPTDAYGDLDFTYSGRKHSNFLRLSDRTDPATVYSLVTRSWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQTASTGSSKVVAMGVAPWGVVRNRDMLINPKGSFPARYRWRGDPEDGVEFPLDYNYSAFFLVDDGTYGRLGGENRFRLRFESYVAQQKTGVGGTGIDIPVLLLLIDGDEKMLKRIEDATQAQLPCLLVAGSGGAADCLVETLEDTLAPGSGGLRRGEARDRIRRYFPKGDPEVLQAQVERIMTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSPPVNGTVELRPPNVGQVLRTLLGETCAPRYPARNTRDSYLGQDHRENDSLLMDWANKQPSTDASFEQAPWSDLLIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLESEAEEAARRKDLAATFESMSVDLFGECYHNSEERAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTTPIWALLLAFFCPPLIYTNLIVFRKSEEEPTQKDLDFDMDSSINGAGPPGTVEPSAKVALERRQRRRPGRALCCGKFSKRWSDFWGAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPSVSELLLYFWAFTLLCEELRQGLGGGWGSLASGGRGPDRAPLRHRLHLYLSDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNKQLGPKIVIVSKMMKDVFFFLFFLCVWLVAYGVATEGILRPQDRSLPSILRRVFYRPYLQIFGQIPQEEMDVALMIPGNCSMERGSWAHPEGPVAGSCVSQYANWLVVLLLIVFLLVANILLLNLLIAMFSYTFSKVHGNSDLYWKAQRYSLIREFHSRPALAPPLIIISHVRLLIKWLRRCRRCRRANLPASPVFEHFRVCLSKEAERKLLTWESVHKENFLLAQARDKRDSDSERLKRTSQKVDTALKQLGQIREYDRRLRGLEREVQHCSRVLTWMAEALSHSALLPPGAPPPPSPTGSKD

Target ORF information:

RefSeq Version	NM_175130.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175130.4

ORF Insert Sequence:

ATGGTGGGGC CGGAGAAGGA GCAGAGCTGG ATCCCTAAGA TCTTCAGGAA GAAGGTGTGC 
ACGACGTTCA TAGTGGACCT CTCCGATGAT GCGGGAGGGA CCTTGTGCCA GTGTGGGCAA 
CCTCGGGATG CCCACCCTTC TGTGGCTGTG GAGGATGCCT TTGGGGCAGC CGTAGTTACC 
GAGTGGAACA GTGATGAGCA CACCACGGAG AAGCCCACAG ATGCCTATGG GGACCTGGAC 
TTCACGTACT CTGGCCGAAA GCACAGCAAC TTTCTCCGGC TGTCTGACCG CACGGATCCG 
GCCACTGTGT ACAGTCTCGT TACCCGCTCT TGGGGCTTTC GTGCCCCAAA CTTGGTGGTG 
TCGGTGCTGG GGGGGTCGGG GGGCCCCGTG CTCCAGACCT GGCTGCAGGA CCTTCTGCGT 
CGCGGGCTGG TGAGAGCCGC CCAGAGCACA GGGGCCTGGA TCGTCACTGG GGGACTGCAC 
ACAGGCATTG GCCGGCACGT GGGTGTGGCT GTAAGGGACC ACCAGACAGC CAGCACTGGG 
AGCAGCAAGG TGGTGGCCAT GGGTGTGGCC CCCTGGGGAG TGGTCCGGAA CAGAGACATG 
CTGATCAACC CCAAGGGCTC ATTCCCCGCA AGGTACCGGT GGCGTGGTGA CCCTGAGGAT 
GGTGTTGAGT TCCCCCTGGA CTATAACTAT TCTGCTTTCT TCTTGGTGGA TGATGGCACC 
TATGGCCGCC TGGGTGGTGA GAACCGCTTC CGCCTTCGGT TTGAGTCCTA TGTGGCTCAG 
CAGAAGACCG GAGTGGGAGG GACTGGAATT GACATCCCAG TGCTCCTTCT TCTCATCGAT 
GGAGATGAGA AGATGTTAAA GCGGATAGAG GATGCCACCC AAGCTCAGCT CCCCTGCCTC 
TTGGTGGCGG GCTCTGGGGG TGCTGCAGAC TGTCTGGTGG AGACCCTGGA AGATACTCTG 
GCCCCAGGGA GTGGGGGACT TAGGCGAGGC GAAGCCCGGG ATCGGATTAG GCGATACTTC 
CCTAAAGGAG ACCCTGAGGT TCTTCAGGCC CAGGTAGAGA GGATCATGAC CCGAAAGGAG 
TTGCTGACGG TCTATTCATC AGAAGACGGC TCCGAGGAGT TTGAGACTAT CGTTTTGAGG 
GCTCTTGTGA AAGCCTGTGG GAGCTCTGAG GCCTCAGCCT ACCTGGATGA GCTGCGTTTG 
GCTGTGGCTT GGAACCGCGT GGACATCGCC CAAAGTGAAC TTTTCCGTGG GGACATCCAA 
TGGCGGTCCT TCCACCTGGA GGCATCTCTC ATGGATGCTC TGCTGAATGA CCGGCCTGAA 
TTTGTGCGCC TGCTCATCTC CCATGGCCTT AGCCTGGGAC ACTTCCTGAC CCCCGTGCGT 
CTGGCCCAGC TGTACAGCGC GGTGTCCCCT AACTCACTGA TCCGAAATCT TCTGGACCAG 
GCGTCCCATG CTAGTAGCAG CAAATCCCCT CCTGTAAATG GAACTGTAGA GCTCCGGCCT 
CCCAATGTGG GGCAAGTGCT GAGGACTCTG TTGGGAGAAA CGTGTGCACC ACGGTACCCT 
GCCAGGAACA CCCGAGACTC CTATCTGGGC CAGGACCACA GAGAGAATGA CTCTCTGCTT 
ATGGACTGGG CCAACAAGCA ACCATCAACG GATGCAAGCT TTGAACAAGC CCCCTGGAGT 
GACCTGCTTA TTTGGGCTCT GTTGCTGAAC CGGGCCCAGA TGGCTATCTA TTTCTGGGAG 
AAGGGCTCCA ACTCAGTGGC CTCTGCTCTT GGGGCTTGTC TTTTACTCCG AGTGATGGCT 
CGCCTAGAGT CGGAGGCTGA GGAGGCCGCG CGGCGGAAGG ATCTTGCTGC CACGTTTGAA 
AGCATGAGCG TGGACCTCTT TGGAGAGTGT TACCACAACA GTGAAGAGCG AGCAGCCCGC 
CTTCTTCTGC GCCGCTGTCC CCTCTGGGGA GAGGCCACCT GCCTCCAGCT TGCCATGCAG 
GCTGATGCCC GAGCGTTCTT TGCCCAGGAT GGAGTCCAGT CTCTTCTGAC ACAGAAGTGG 
TGGGGGGAGA TGGACAGCAC AACTCCCATC TGGGCCCTGC TTCTCGCCTT CTTCTGCCCG 
CCTCTCATCT ACACCAACCT TATCGTCTTC AGGAAGTCAG AGGAGGAGCC CACACAGAAG 
GATCTTGACT TTGATATGGA CAGCAGCATC AATGGAGCAG GTCCTCCTGG GACTGTAGAA 
CCCTCTGCAA AGGTGGCCCT TGAGAGGCGG CAGCGGCGAC GGCCAGGACG TGCCCTCTGC 
TGTGGCAAGT TCTCCAAGCG CTGGTCGGAC TTCTGGGGTG CCCCAGTGAC TGCCTTCCTG 
GGTAATGTGG TCAGTTACCT GCTGTTCCTG CTGCTGTTTG CCCACGTGTT GCTGGTGGAT 
TTCCAGCCCA CAAAGCCAAG TGTCTCCGAG CTGCTGCTGT ACTTCTGGGC CTTCACGTTG 
CTGTGCGAGG AGCTGCGCCA GGGCCTGGGT GGTGGCTGGG GTAGCCTGGC CAGTGGGGGA 
CGTGGTCCGG ACAGAGCTCC CCTGCGCCAT CGGCTGCACC TCTACCTCTC AGATACCTGG 
AACCAATGTG ACCTGCTGGC ACTCACCTGC TTCCTGCTGG GCGTAGGCTG CCGGCTGACC 
CCTGGGCTCT TTGACTTGGG ACGCACCGTC CTCTGCCTTG ACTTCATGAT CTTCACACTG 
CGCCTGCTGC ACATCTTCAC GGTGAACAAG CAGCTGGGGC CCAAGATTGT CATAGTGAGC 
AAAATGATGA AGGATGTATT CTTCTTCCTC TTCTTCCTCT GCGTGTGGCT TGTAGCTTAT 
GGGGTGGCCA CAGAGGGGAT CCTGAGGCCC CAGGACCGCA GTCTACCGAG TATCCTGCGC 
AGGGTCTTCT ACCGACCGTA TCTGCAGATC TTCGGACAAA TTCCCCAGGA GGAAATGGAC 
GTGGCCCTCA TGATTCCAGG TAACTGCTCA ATGGAGCGGG GCTCCTGGGC TCATCCGGAG 
GGGCCCGTCG CAGGCTCCTG TGTGTCCCAG TATGCCAACT GGCTGGTGGT GTTGCTCCTT 
ATCGTCTTCT TGCTGGTGGC CAATATCCTG TTGCTCAACC TGCTCATCGC CATGTTCAGC 
TACACCTTCA GCAAAGTGCA CGGCAACAGC GACCTCTACT GGAAGGCACA GCGCTACAGC 
CTCATCCGGG AATTCCATTC GAGGCCTGCC CTGGCCCCAC CCCTCATCAT CATCTCTCAT 
GTGCGCCTCC TCATCAAGTG GCTGCGCAGG TGTCGCAGGT GTCGCAGGGC TAACTTGCCC 
GCCTCCCCAG TCTTCGAGCA CTTCCGTGTC TGTCTCTCTA AGGAAGCAGA GCGGAAGCTG 
CTGACTTGGG AGTCTGTGCA CAAAGAGAAC TTCCTGTTGG CACAAGCTCG GGACAAGCGA 
GACAGTGACT CGGAGCGTTT GAAACGCACG TCTCAGAAGG TGGACACTGC ACTGAAGCAG 
CTGGGACAGA TCAGAGAGTA CGATCGGCGT CTGAGGGGGC TGGAGAGAGA GGTCCAACAC 
TGTTCTCGAG TCCTGACTTG GATGGCTGAG GCCCTTAGCC ACTCTGCCTT GCTGCCTCCA 
GGGGCCCCAC CCCCTCCGAG CCCCACTGGT TCCAAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm4 ( XM_006541146.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541209.1 Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X5, mRNA.

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CloneID	OMu38998
Clone ID Related Accession (Same CDS sequence)	XM_006541146.3 , XM_006541146.4
Accession Version	XM_006541146.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006541146.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGGG GGGCCTGGAT CGTCACTGGG GGACTGCACA CAGGCATTGG CCGGCACGTG 
GGTGTGGCTG TAAGGGACCA CCAGACAGCC AGCACTGGGA GCAGCAAGGT GGTGGCCATG 
GGTGTGGCCC CCTGGGGAGT GGTCCGGAAC AGAGACATGC TGATCAACCC CAAGGGCTCA 
TTCCCCGCAA GGTACCGGTG GCGTGGTGAC CCTGAGGATG GTGTTGAGTT CCCCCTGGAC 
TATAACTATT CTGCTTTCTT CTTGGTGGAT GATGGCACCT ATGGCCGCCT GGGTGGTGAG 
AACCGCTTCC GCCTTCGGTT TGAGTCCTAT GTGGCTCAGC AGAAGACCGG AGTGGGAGGG 
ACTGGAATTG ACATCCCAGT GCTCCTTCTT CTCATCGATG GAGATGAGAA GATGTTAAAG 
CGGATAGAGG ATGCCACCCA AGCTCAGCTC CCCTGCCTCT TGGTGGCGGG CTCTGGGGGT 
GCTGCAGACT GTCTGGTGGA GACCCTGGAA GATACTCTGG CCCCAGGGAG TGGGGGACTT 
AGGCGAGGCG AAGCCCGGGA TCGGATTAGG CGATACTTCC CTAAAGGAGA CCCTGAGGTT 
CTTCAGGCCC AGGTAGAGAG GATCATGACC CGAAAGGAGT TGCTGACGGT CTATTCATCA 
GAAGACGGCT CCGAGGAGTT TGAGACTATC GTTTTGAGGG CTCTTGTGAA AGCCTGTGGG 
AGCTCTGAGG CCTCAGCCTA CCTGGATGAG CTGCGTTTGG CTGTGGCTTG GAACCGCGTG 
GACATCGCCC AAAGTGAACT TTTCCGTGGG GACATCCAAT GGCGGTCCTT CCACCTGGAG 
GCATCTCTCA TGGATGCTCT GCTGAATGAC CGGCCTGAAT TTGTGCGCCT GCTCATCTCC 
CATGGCCTTA GCCTGGGACA CTTCCTGACC CCCGTGCGTC TGGCCCAGCT GTACAGCGCG 
GTGTCCCCTA ACTCACTGAT CCGAAATCTT CTGGACCAGG CGTCCCATGC TAGTAGCAGC 
AAATCCCCTC CTGTAAATGG AACTGTAGAG CTCCGGCCTC CCAATGTGGG GCAAGTGCTG 
AGGACTCTGT TGGGAGAAAC GTGTGCACCA CGGTACCCTG CCAGGAACAC CCGAGACTCC 
TATCTGGGCC AGGACCACAG AGAGAATGAC TCTCTGCTTA TGGACTGGGC CAACAAGCAA 
CCATCAACGG ATGCAAGCTT TGAACAAGCC CCCTGGAGTG ACCTGCTTAT TTGGGCTCTG 
TTGCTGAACC GGGCCCAGAT GGCTATCTAT TTCTGGGAGA AGGGCTCCAA CTCAGTGGCC 
TCTGCTCTTG GGGCTTGTCT TTTACTCCGA GTGATGGCTC GCCTAGAGTC GGAGGCTGAG 
GAGGCCGCGC GGCGGAAGGA TCTTGCTGCC ACGTTTGAAA GCATGAGCGT GGACCTCTTT 
GGAGAGTGTT ACCACAACAG TGAAGAGCGA GCAGCCCGCC TTCTTCTGCG CCGCTGTCCC 
CTCTGGGGAG AGGCCACCTG CCTCCAGCTT GCCATGCAGG CTGATGCCCG AGCGTTCTTT 
GCCCAGGATG GAGTCCAGTC TCTTCTGACA CAGAAGTGGT GGGGGGAGAT GGACAGCACA 
ACTCCCATCT GGGCCCTGCT TCTCGCCTTC TTCTGCCCGC CTCTCATCTA CACCAACCTT 
ATCGTCTTCA GGAAGTCAGA GGAGGAGCCC ACACAGAAGG ATCTTGACTT TGATATGGAC 
AGCAGCATCA ATGGAGCAGG TCCTCCTGGG ACTGTAGAAC CCTCTGCAAA GGTGGCCCTT 
GAGAGGCGGC AGCGGCGACG GCCAGGACGT GCCCTCTGCT GTGGCAAGTT CTCCAAGCGC 
TGGTCGGACT TCTGGGGTGC CCCAGTGACT GCCTTCCTGG GTAATGTGGT CAGTTACCTG 
CTGTTCCTGC TGCTGTTTGC CCACGTGTTG CTGGTGGATT TCCAGCCCAC AAAGCCAAGT 
GTCTCCGAGC TGCTGCTGTA CTTCTGGGCC TTCACGTTGC TGTGCGAGGA GCTGCGCCAG 
GGCCTGGGTG GTGGCTGGGG TAGCCTGGCC AGTGGGGGAC GTGGTCCGGA CAGAGCTCCC 
CTGCGCCATC GGCTGCACCT CTACCTCTCA GATACCTGGA ACCAATGTGA CCTGCTGGCA 
CTCACCTGCT TCCTGCTGGG CGTAGGCTGC CGGCTGACCC CTGGGCTCTT TGACTTGGGA 
CGCACCGTCC TCTGCCTTGA CTTCATGATC TTCACACTGC GCCTGCTGCA CATCTTCACG 
GTGAACAAGC AGCTGGGGCC CAAGATTGTC ATAGTGAGCA AAATGATGAA GGATGTATTC 
TTCTTCCTCT TCTTCCTCTG CGTGTGGCTT GTAGCTTATG GGGTGGCCAC AGAGGGGATC 
CTGAGGCCCC AGGACCGCAG TCTACCGAGT ATCCTGCGCA GGGTCTTCTA CCGACCGTAT 
CTGCAGATCT TCGGACAAAT TCCCCAGGAG GAAATGGACG TGGCCCTCAT GATTCCAGGT 
AACTGCTCAA TGGAGCGGGG CTCCTGGGCT CATCCGGAGG GGCCCGTCGC AGGCTCCTGT 
GTGTCCCAGT ATGCCAACTG GCTGGTGGTG TTGCTCCTTA TCGTCTTCTT GCTGGTGGCC 
AATATCCTGT TGCTCAACCT GCTCATCGCC ATGTTCAGCT ACACCTTCAG CAAAGTGCAC 
GGCAACAGCG ACCTCTACTG GAAGGCACAG CGCTACAGCC TCATCCGGGA ATTCCATTCG 
AGGCCTGCCC TGGCCCCACC CCTCATCATC ATCTCTCATG TGCGCCTCCT CATCAAGTGG 
CTGCGCAGGT GTCGCAGGTG TCGCAGGGCT AACTTGCCCG CCTCCCCAGT CTTCGAGCAC 
TTCCGTGTCT GTCTCTCTAA GGAAGCAGAG CGGAAGCTGC TGACTTGGGA GTCTGTGCAC 
AAAGAGAACT TCCTGTTGGC ACAAGCTCGG GACAAGCGAG ACAGTGACTC GGAGCGTTTG 
AAACGCACGT CTCAGAAGGT GGACACTGCA CTGAAGCAGC TGGGACAGAT CAGAGAGTAC 
GATCGGCGTC TGAGGGGGCT GGAGAGAGAG GTCCAACACT GTTCTCGAGT CCTGACTTGG 
ATGGCTGAGG CCCTTAGCCA CTCTGCCTTG CTGCCTCCAG GGGCCCCACC CCCTCCGAGC 
CCCACTGGTT CCAAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541209.1
CDS	442..3642
Translation	MMRGAWIVTGGLHTGIGRHVGVAVRDHQTASTGSSKVVAMGVAPWGVVRNRDMLINPKGSFPARYRWRGDPEDGVEFPLDYNYSAFFLVDDGTYGRLGGENRFRLRFESYVAQQKTGVGGTGIDIPVLLLLIDGDEKMLKRIEDATQAQLPCLLVAGSGGAADCLVETLEDTLAPGSGGLRRGEARDRIRRYFPKGDPEVLQAQVERIMTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSPPVNGTVELRPPNVGQVLRTLLGETCAPRYPARNTRDSYLGQDHRENDSLLMDWANKQPSTDASFEQAPWSDLLIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLESEAEEAARRKDLAATFESMSVDLFGECYHNSEERAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTTPIWALLLAFFCPPLIYTNLIVFRKSEEEPTQKDLDFDMDSSINGAGPPGTVEPSAKVALERRQRRRPGRALCCGKFSKRWSDFWGAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPSVSELLLYFWAFTLLCEELRQGLGGGWGSLASGGRGPDRAPLRHRLHLYLSDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNKQLGPKIVIVSKMMKDVFFFLFFLCVWLVAYGVATEGILRPQDRSLPSILRRVFYRPYLQIFGQIPQEEMDVALMIPGNCSMERGSWAHPEGPVAGSCVSQYANWLVVLLLIVFLLVANILLLNLLIAMFSYTFSKVHGNSDLYWKAQRYSLIREFHSRPALAPPLIIISHVRLLIKWLRRCRRCRRANLPASPVFEHFRVCLSKEAERKLLTWESVHKENFLLAQARDKRDSDSERLKRTSQKVDTALKQLGQIREYDRRLRGLEREVQHCSRVLTWMAEALSHSALLPPGAPPPPSPTGSKD

Target ORF information:

RefSeq Version	XM_006541146.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541146.4

ORF Insert Sequence:

ATGATGCGGG GGGCCTGGAT CGTCACTGGG GGACTGCACA CAGGCATTGG CCGGCACGTG 
GGTGTGGCTG TAAGGGACCA CCAGACAGCC AGCACTGGGA GCAGCAAGGT GGTGGCCATG 
GGTGTGGCCC CCTGGGGAGT GGTCCGGAAC AGAGACATGC TGATCAACCC CAAGGGCTCA 
TTCCCCGCAA GGTACCGGTG GCGTGGTGAC CCTGAGGATG GTGTTGAGTT CCCCCTGGAC 
TATAACTATT CTGCTTTCTT CTTGGTGGAT GATGGCACCT ATGGCCGCCT GGGTGGTGAG 
AACCGCTTCC GCCTTCGGTT TGAGTCCTAT GTGGCTCAGC AGAAGACCGG AGTGGGAGGG 
ACTGGAATTG ACATCCCAGT GCTCCTTCTT CTCATCGATG GAGATGAGAA GATGTTAAAG 
CGGATAGAGG ATGCCACCCA AGCTCAGCTC CCCTGCCTCT TGGTGGCGGG CTCTGGGGGT 
GCTGCAGACT GTCTGGTGGA GACCCTGGAA GATACTCTGG CCCCAGGGAG TGGGGGACTT 
AGGCGAGGCG AAGCCCGGGA TCGGATTAGG CGATACTTCC CTAAAGGAGA CCCTGAGGTT 
CTTCAGGCCC AGGTAGAGAG GATCATGACC CGAAAGGAGT TGCTGACGGT CTATTCATCA 
GAAGACGGCT CCGAGGAGTT TGAGACTATC GTTTTGAGGG CTCTTGTGAA AGCCTGTGGG 
AGCTCTGAGG CCTCAGCCTA CCTGGATGAG CTGCGTTTGG CTGTGGCTTG GAACCGCGTG 
GACATCGCCC AAAGTGAACT TTTCCGTGGG GACATCCAAT GGCGGTCCTT CCACCTGGAG 
GCATCTCTCA TGGATGCTCT GCTGAATGAC CGGCCTGAAT TTGTGCGCCT GCTCATCTCC 
CATGGCCTTA GCCTGGGACA CTTCCTGACC CCCGTGCGTC TGGCCCAGCT GTACAGCGCG 
GTGTCCCCTA ACTCACTGAT CCGAAATCTT CTGGACCAGG CGTCCCATGC TAGTAGCAGC 
AAATCCCCTC CTGTAAATGG AACTGTAGAG CTCCGGCCTC CCAATGTGGG GCAAGTGCTG 
AGGACTCTGT TGGGAGAAAC GTGTGCACCA CGGTACCCTG CCAGGAACAC CCGAGACTCC 
TATCTGGGCC AGGACCACAG AGAGAATGAC TCTCTGCTTA TGGACTGGGC CAACAAGCAA 
CCATCAACGG ATGCAAGCTT TGAACAAGCC CCCTGGAGTG ACCTGCTTAT TTGGGCTCTG 
TTGCTGAACC GGGCCCAGAT GGCTATCTAT TTCTGGGAGA AGGGCTCCAA CTCAGTGGCC 
TCTGCTCTTG GGGCTTGTCT TTTACTCCGA GTGATGGCTC GCCTAGAGTC GGAGGCTGAG 
GAGGCCGCGC GGCGGAAGGA TCTTGCTGCC ACGTTTGAAA GCATGAGCGT GGACCTCTTT 
GGAGAGTGTT ACCACAACAG TGAAGAGCGA GCAGCCCGCC TTCTTCTGCG CCGCTGTCCC 
CTCTGGGGAG AGGCCACCTG CCTCCAGCTT GCCATGCAGG CTGATGCCCG AGCGTTCTTT 
GCCCAGGATG GAGTCCAGTC TCTTCTGACA CAGAAGTGGT GGGGGGAGAT GGACAGCACA 
ACTCCCATCT GGGCCCTGCT TCTCGCCTTC TTCTGCCCGC CTCTCATCTA CACCAACCTT 
ATCGTCTTCA GGAAGTCAGA GGAGGAGCCC ACACAGAAGG ATCTTGACTT TGATATGGAC 
AGCAGCATCA ATGGAGCAGG TCCTCCTGGG ACTGTAGAAC CCTCTGCAAA GGTGGCCCTT 
GAGAGGCGGC AGCGGCGACG GCCAGGACGT GCCCTCTGCT GTGGCAAGTT CTCCAAGCGC 
TGGTCGGACT TCTGGGGTGC CCCAGTGACT GCCTTCCTGG GTAATGTGGT CAGTTACCTG 
CTGTTCCTGC TGCTGTTTGC CCACGTGTTG CTGGTGGATT TCCAGCCCAC AAAGCCAAGT 
GTCTCCGAGC TGCTGCTGTA CTTCTGGGCC TTCACGTTGC TGTGCGAGGA GCTGCGCCAG 
GGCCTGGGTG GTGGCTGGGG TAGCCTGGCC AGTGGGGGAC GTGGTCCGGA CAGAGCTCCC 
CTGCGCCATC GGCTGCACCT CTACCTCTCA GATACCTGGA ACCAATGTGA CCTGCTGGCA 
CTCACCTGCT TCCTGCTGGG CGTAGGCTGC CGGCTGACCC CTGGGCTCTT TGACTTGGGA 
CGCACCGTCC TCTGCCTTGA CTTCATGATC TTCACACTGC GCCTGCTGCA CATCTTCACG 
GTGAACAAGC AGCTGGGGCC CAAGATTGTC ATAGTGAGCA AAATGATGAA GGATGTATTC 
TTCTTCCTCT TCTTCCTCTG CGTGTGGCTT GTAGCTTATG GGGTGGCCAC AGAGGGGATC 
CTGAGGCCCC AGGACCGCAG TCTACCGAGT ATCCTGCGCA GGGTCTTCTA CCGACCGTAT 
CTGCAGATCT TCGGACAAAT TCCCCAGGAG GAAATGGACG TGGCCCTCAT GATTCCAGGT 
AACTGCTCAA TGGAGCGGGG CTCCTGGGCT CATCCGGAGG GGCCCGTCGC AGGCTCCTGT 
GTGTCCCAGT ATGCCAACTG GCTGGTGGTG TTGCTCCTTA TCGTCTTCTT GCTGGTGGCC 
AATATCCTGT TGCTCAACCT GCTCATCGCC ATGTTCAGCT ACACCTTCAG CAAAGTGCAC 
GGCAACAGCG ACCTCTACTG GAAGGCACAG CGCTACAGCC TCATCCGGGA ATTCCATTCG 
AGGCCTGCCC TGGCCCCACC CCTCATCATC ATCTCTCATG TGCGCCTCCT CATCAAGTGG 
CTGCGCAGGT GTCGCAGGTG TCGCAGGGCT AACTTGCCCG CCTCCCCAGT CTTCGAGCAC 
TTCCGTGTCT GTCTCTCTAA GGAAGCAGAG CGGAAGCTGC TGACTTGGGA GTCTGTGCAC 
AAAGAGAACT TCCTGTTGGC ACAAGCTCGG GACAAGCGAG ACAGTGACTC GGAGCGTTTG 
AAACGCACGT CTCAGAAGGT GGACACTGCA CTGAAGCAGC TGGGACAGAT CAGAGAGTAC 
GATCGGCGTC TGAGGGGGCT GGAGAGAGAG GTCCAACACT GTTCTCGAGT CCTGACTTGG 
ATGGCTGAGG CCCTTAGCCA CTCTGCCTTG CTGCCTCCAG GGGCCCCACC CCCTCCGAGC 
CCCACTGGTT CCAAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm4 ( XM_006541146.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541209.1 Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu38998
Clone ID Related Accession (Same CDS sequence)	XM_006541146.3 , XM_006541146.4
Accession Version	XM_006541146.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006541146.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGGG GGGCCTGGAT CGTCACTGGG GGACTGCACA CAGGCATTGG CCGGCACGTG 
GGTGTGGCTG TAAGGGACCA CCAGACAGCC AGCACTGGGA GCAGCAAGGT GGTGGCCATG 
GGTGTGGCCC CCTGGGGAGT GGTCCGGAAC AGAGACATGC TGATCAACCC CAAGGGCTCA 
TTCCCCGCAA GGTACCGGTG GCGTGGTGAC CCTGAGGATG GTGTTGAGTT CCCCCTGGAC 
TATAACTATT CTGCTTTCTT CTTGGTGGAT GATGGCACCT ATGGCCGCCT GGGTGGTGAG 
AACCGCTTCC GCCTTCGGTT TGAGTCCTAT GTGGCTCAGC AGAAGACCGG AGTGGGAGGG 
ACTGGAATTG ACATCCCAGT GCTCCTTCTT CTCATCGATG GAGATGAGAA GATGTTAAAG 
CGGATAGAGG ATGCCACCCA AGCTCAGCTC CCCTGCCTCT TGGTGGCGGG CTCTGGGGGT 
GCTGCAGACT GTCTGGTGGA GACCCTGGAA GATACTCTGG CCCCAGGGAG TGGGGGACTT 
AGGCGAGGCG AAGCCCGGGA TCGGATTAGG CGATACTTCC CTAAAGGAGA CCCTGAGGTT 
CTTCAGGCCC AGGTAGAGAG GATCATGACC CGAAAGGAGT TGCTGACGGT CTATTCATCA 
GAAGACGGCT CCGAGGAGTT TGAGACTATC GTTTTGAGGG CTCTTGTGAA AGCCTGTGGG 
AGCTCTGAGG CCTCAGCCTA CCTGGATGAG CTGCGTTTGG CTGTGGCTTG GAACCGCGTG 
GACATCGCCC AAAGTGAACT TTTCCGTGGG GACATCCAAT GGCGGTCCTT CCACCTGGAG 
GCATCTCTCA TGGATGCTCT GCTGAATGAC CGGCCTGAAT TTGTGCGCCT GCTCATCTCC 
CATGGCCTTA GCCTGGGACA CTTCCTGACC CCCGTGCGTC TGGCCCAGCT GTACAGCGCG 
GTGTCCCCTA ACTCACTGAT CCGAAATCTT CTGGACCAGG CGTCCCATGC TAGTAGCAGC 
AAATCCCCTC CTGTAAATGG AACTGTAGAG CTCCGGCCTC CCAATGTGGG GCAAGTGCTG 
AGGACTCTGT TGGGAGAAAC GTGTGCACCA CGGTACCCTG CCAGGAACAC CCGAGACTCC 
TATCTGGGCC AGGACCACAG AGAGAATGAC TCTCTGCTTA TGGACTGGGC CAACAAGCAA 
CCATCAACGG ATGCAAGCTT TGAACAAGCC CCCTGGAGTG ACCTGCTTAT TTGGGCTCTG 
TTGCTGAACC GGGCCCAGAT GGCTATCTAT TTCTGGGAGA AGGGCTCCAA CTCAGTGGCC 
TCTGCTCTTG GGGCTTGTCT TTTACTCCGA GTGATGGCTC GCCTAGAGTC GGAGGCTGAG 
GAGGCCGCGC GGCGGAAGGA TCTTGCTGCC ACGTTTGAAA GCATGAGCGT GGACCTCTTT 
GGAGAGTGTT ACCACAACAG TGAAGAGCGA GCAGCCCGCC TTCTTCTGCG CCGCTGTCCC 
CTCTGGGGAG AGGCCACCTG CCTCCAGCTT GCCATGCAGG CTGATGCCCG AGCGTTCTTT 
GCCCAGGATG GAGTCCAGTC TCTTCTGACA CAGAAGTGGT GGGGGGAGAT GGACAGCACA 
ACTCCCATCT GGGCCCTGCT TCTCGCCTTC TTCTGCCCGC CTCTCATCTA CACCAACCTT 
ATCGTCTTCA GGAAGTCAGA GGAGGAGCCC ACACAGAAGG ATCTTGACTT TGATATGGAC 
AGCAGCATCA ATGGAGCAGG TCCTCCTGGG ACTGTAGAAC CCTCTGCAAA GGTGGCCCTT 
GAGAGGCGGC AGCGGCGACG GCCAGGACGT GCCCTCTGCT GTGGCAAGTT CTCCAAGCGC 
TGGTCGGACT TCTGGGGTGC CCCAGTGACT GCCTTCCTGG GTAATGTGGT CAGTTACCTG 
CTGTTCCTGC TGCTGTTTGC CCACGTGTTG CTGGTGGATT TCCAGCCCAC AAAGCCAAGT 
GTCTCCGAGC TGCTGCTGTA CTTCTGGGCC TTCACGTTGC TGTGCGAGGA GCTGCGCCAG 
GGCCTGGGTG GTGGCTGGGG TAGCCTGGCC AGTGGGGGAC GTGGTCCGGA CAGAGCTCCC 
CTGCGCCATC GGCTGCACCT CTACCTCTCA GATACCTGGA ACCAATGTGA CCTGCTGGCA 
CTCACCTGCT TCCTGCTGGG CGTAGGCTGC CGGCTGACCC CTGGGCTCTT TGACTTGGGA 
CGCACCGTCC TCTGCCTTGA CTTCATGATC TTCACACTGC GCCTGCTGCA CATCTTCACG 
GTGAACAAGC AGCTGGGGCC CAAGATTGTC ATAGTGAGCA AAATGATGAA GGATGTATTC 
TTCTTCCTCT TCTTCCTCTG CGTGTGGCTT GTAGCTTATG GGGTGGCCAC AGAGGGGATC 
CTGAGGCCCC AGGACCGCAG TCTACCGAGT ATCCTGCGCA GGGTCTTCTA CCGACCGTAT 
CTGCAGATCT TCGGACAAAT TCCCCAGGAG GAAATGGACG TGGCCCTCAT GATTCCAGGT 
AACTGCTCAA TGGAGCGGGG CTCCTGGGCT CATCCGGAGG GGCCCGTCGC AGGCTCCTGT 
GTGTCCCAGT ATGCCAACTG GCTGGTGGTG TTGCTCCTTA TCGTCTTCTT GCTGGTGGCC 
AATATCCTGT TGCTCAACCT GCTCATCGCC ATGTTCAGCT ACACCTTCAG CAAAGTGCAC 
GGCAACAGCG ACCTCTACTG GAAGGCACAG CGCTACAGCC TCATCCGGGA ATTCCATTCG 
AGGCCTGCCC TGGCCCCACC CCTCATCATC ATCTCTCATG TGCGCCTCCT CATCAAGTGG 
CTGCGCAGGT GTCGCAGGTG TCGCAGGGCT AACTTGCCCG CCTCCCCAGT CTTCGAGCAC 
TTCCGTGTCT GTCTCTCTAA GGAAGCAGAG CGGAAGCTGC TGACTTGGGA GTCTGTGCAC 
AAAGAGAACT TCCTGTTGGC ACAAGCTCGG GACAAGCGAG ACAGTGACTC GGAGCGTTTG 
AAACGCACGT CTCAGAAGGT GGACACTGCA CTGAAGCAGC TGGGACAGAT CAGAGAGTAC 
GATCGGCGTC TGAGGGGGCT GGAGAGAGAG GTCCAACACT GTTCTCGAGT CCTGACTTGG 
ATGGCTGAGG CCCTTAGCCA CTCTGCCTTG CTGCCTCCAG GGGCCCCACC CCCTCCGAGC 
CCCACTGGTT CCAAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541209.1
CDS	443..3643
Translation	MMRGAWIVTGGLHTGIGRHVGVAVRDHQTASTGSSKVVAMGVAPWGVVRNRDMLINPKGSFPARYRWRGDPEDGVEFPLDYNYSAFFLVDDGTYGRLGGENRFRLRFESYVAQQKTGVGGTGIDIPVLLLLIDGDEKMLKRIEDATQAQLPCLLVAGSGGAADCLVETLEDTLAPGSGGLRRGEARDRIRRYFPKGDPEVLQAQVERIMTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSPPVNGTVELRPPNVGQVLRTLLGETCAPRYPARNTRDSYLGQDHRENDSLLMDWANKQPSTDASFEQAPWSDLLIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLESEAEEAARRKDLAATFESMSVDLFGECYHNSEERAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTTPIWALLLAFFCPPLIYTNLIVFRKSEEEPTQKDLDFDMDSSINGAGPPGTVEPSAKVALERRQRRRPGRALCCGKFSKRWSDFWGAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPSVSELLLYFWAFTLLCEELRQGLGGGWGSLASGGRGPDRAPLRHRLHLYLSDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNKQLGPKIVIVSKMMKDVFFFLFFLCVWLVAYGVATEGILRPQDRSLPSILRRVFYRPYLQIFGQIPQEEMDVALMIPGNCSMERGSWAHPEGPVAGSCVSQYANWLVVLLLIVFLLVANILLLNLLIAMFSYTFSKVHGNSDLYWKAQRYSLIREFHSRPALAPPLIIISHVRLLIKWLRRCRRCRRANLPASPVFEHFRVCLSKEAERKLLTWESVHKENFLLAQARDKRDSDSERLKRTSQKVDTALKQLGQIREYDRRLRGLEREVQHCSRVLTWMAEALSHSALLPPGAPPPPSPTGSKD

Target ORF information:

RefSeq Version	XM_006541146.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541146.3

ORF Insert Sequence:

ATGATGCGGG GGGCCTGGAT CGTCACTGGG GGACTGCACA CAGGCATTGG CCGGCACGTG 
GGTGTGGCTG TAAGGGACCA CCAGACAGCC AGCACTGGGA GCAGCAAGGT GGTGGCCATG 
GGTGTGGCCC CCTGGGGAGT GGTCCGGAAC AGAGACATGC TGATCAACCC CAAGGGCTCA 
TTCCCCGCAA GGTACCGGTG GCGTGGTGAC CCTGAGGATG GTGTTGAGTT CCCCCTGGAC 
TATAACTATT CTGCTTTCTT CTTGGTGGAT GATGGCACCT ATGGCCGCCT GGGTGGTGAG 
AACCGCTTCC GCCTTCGGTT TGAGTCCTAT GTGGCTCAGC AGAAGACCGG AGTGGGAGGG 
ACTGGAATTG ACATCCCAGT GCTCCTTCTT CTCATCGATG GAGATGAGAA GATGTTAAAG 
CGGATAGAGG ATGCCACCCA AGCTCAGCTC CCCTGCCTCT TGGTGGCGGG CTCTGGGGGT 
GCTGCAGACT GTCTGGTGGA GACCCTGGAA GATACTCTGG CCCCAGGGAG TGGGGGACTT 
AGGCGAGGCG AAGCCCGGGA TCGGATTAGG CGATACTTCC CTAAAGGAGA CCCTGAGGTT 
CTTCAGGCCC AGGTAGAGAG GATCATGACC CGAAAGGAGT TGCTGACGGT CTATTCATCA 
GAAGACGGCT CCGAGGAGTT TGAGACTATC GTTTTGAGGG CTCTTGTGAA AGCCTGTGGG 
AGCTCTGAGG CCTCAGCCTA CCTGGATGAG CTGCGTTTGG CTGTGGCTTG GAACCGCGTG 
GACATCGCCC AAAGTGAACT TTTCCGTGGG GACATCCAAT GGCGGTCCTT CCACCTGGAG 
GCATCTCTCA TGGATGCTCT GCTGAATGAC CGGCCTGAAT TTGTGCGCCT GCTCATCTCC 
CATGGCCTTA GCCTGGGACA CTTCCTGACC CCCGTGCGTC TGGCCCAGCT GTACAGCGCG 
GTGTCCCCTA ACTCACTGAT CCGAAATCTT CTGGACCAGG CGTCCCATGC TAGTAGCAGC 
AAATCCCCTC CTGTAAATGG AACTGTAGAG CTCCGGCCTC CCAATGTGGG GCAAGTGCTG 
AGGACTCTGT TGGGAGAAAC GTGTGCACCA CGGTACCCTG CCAGGAACAC CCGAGACTCC 
TATCTGGGCC AGGACCACAG AGAGAATGAC TCTCTGCTTA TGGACTGGGC CAACAAGCAA 
CCATCAACGG ATGCAAGCTT TGAACAAGCC CCCTGGAGTG ACCTGCTTAT TTGGGCTCTG 
TTGCTGAACC GGGCCCAGAT GGCTATCTAT TTCTGGGAGA AGGGCTCCAA CTCAGTGGCC 
TCTGCTCTTG GGGCTTGTCT TTTACTCCGA GTGATGGCTC GCCTAGAGTC GGAGGCTGAG 
GAGGCCGCGC GGCGGAAGGA TCTTGCTGCC ACGTTTGAAA GCATGAGCGT GGACCTCTTT 
GGAGAGTGTT ACCACAACAG TGAAGAGCGA GCAGCCCGCC TTCTTCTGCG CCGCTGTCCC 
CTCTGGGGAG AGGCCACCTG CCTCCAGCTT GCCATGCAGG CTGATGCCCG AGCGTTCTTT 
GCCCAGGATG GAGTCCAGTC TCTTCTGACA CAGAAGTGGT GGGGGGAGAT GGACAGCACA 
ACTCCCATCT GGGCCCTGCT TCTCGCCTTC TTCTGCCCGC CTCTCATCTA CACCAACCTT 
ATCGTCTTCA GGAAGTCAGA GGAGGAGCCC ACACAGAAGG ATCTTGACTT TGATATGGAC 
AGCAGCATCA ATGGAGCAGG TCCTCCTGGG ACTGTAGAAC CCTCTGCAAA GGTGGCCCTT 
GAGAGGCGGC AGCGGCGACG GCCAGGACGT GCCCTCTGCT GTGGCAAGTT CTCCAAGCGC 
TGGTCGGACT TCTGGGGTGC CCCAGTGACT GCCTTCCTGG GTAATGTGGT CAGTTACCTG 
CTGTTCCTGC TGCTGTTTGC CCACGTGTTG CTGGTGGATT TCCAGCCCAC AAAGCCAAGT 
GTCTCCGAGC TGCTGCTGTA CTTCTGGGCC TTCACGTTGC TGTGCGAGGA GCTGCGCCAG 
GGCCTGGGTG GTGGCTGGGG TAGCCTGGCC AGTGGGGGAC GTGGTCCGGA CAGAGCTCCC 
CTGCGCCATC GGCTGCACCT CTACCTCTCA GATACCTGGA ACCAATGTGA CCTGCTGGCA 
CTCACCTGCT TCCTGCTGGG CGTAGGCTGC CGGCTGACCC CTGGGCTCTT TGACTTGGGA 
CGCACCGTCC TCTGCCTTGA CTTCATGATC TTCACACTGC GCCTGCTGCA CATCTTCACG 
GTGAACAAGC AGCTGGGGCC CAAGATTGTC ATAGTGAGCA AAATGATGAA GGATGTATTC 
TTCTTCCTCT TCTTCCTCTG CGTGTGGCTT GTAGCTTATG GGGTGGCCAC AGAGGGGATC 
CTGAGGCCCC AGGACCGCAG TCTACCGAGT ATCCTGCGCA GGGTCTTCTA CCGACCGTAT 
CTGCAGATCT TCGGACAAAT TCCCCAGGAG GAAATGGACG TGGCCCTCAT GATTCCAGGT 
AACTGCTCAA TGGAGCGGGG CTCCTGGGCT CATCCGGAGG GGCCCGTCGC AGGCTCCTGT 
GTGTCCCAGT ATGCCAACTG GCTGGTGGTG TTGCTCCTTA TCGTCTTCTT GCTGGTGGCC 
AATATCCTGT TGCTCAACCT GCTCATCGCC ATGTTCAGCT ACACCTTCAG CAAAGTGCAC 
GGCAACAGCG ACCTCTACTG GAAGGCACAG CGCTACAGCC TCATCCGGGA ATTCCATTCG 
AGGCCTGCCC TGGCCCCACC CCTCATCATC ATCTCTCATG TGCGCCTCCT CATCAAGTGG 
CTGCGCAGGT GTCGCAGGTG TCGCAGGGCT AACTTGCCCG CCTCCCCAGT CTTCGAGCAC 
TTCCGTGTCT GTCTCTCTAA GGAAGCAGAG CGGAAGCTGC TGACTTGGGA GTCTGTGCAC 
AAAGAGAACT TCCTGTTGGC ACAAGCTCGG GACAAGCGAG ACAGTGACTC GGAGCGTTTG 
AAACGCACGT CTCAGAAGGT GGACACTGCA CTGAAGCAGC TGGGACAGAT CAGAGAGTAC 
GATCGGCGTC TGAGGGGGCT GGAGAGAGAG GTCCAACACT GTTCTCGAGT CCTGACTTGG 
ATGGCTGAGG CCCTTAGCCA CTCTGCCTTG CTGCCTCCAG GGGCCCCACC CCCTCCGAGC 
CCCACTGGTT CCAAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm4 ( XM_006541148.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541211.1 Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X7, mRNA.

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CloneID	OMu39000
Clone ID Related Accession (Same CDS sequence)	XM_006541148.3
Accession Version	XM_006541148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006541148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTCC CATGGCTGAC ATCTATCGTG TGCCCCACTC TGCTTCCTTC GTTCCTCCGC 
TTGCTCTGCC TTCCAACTCT CGGTCTTTCT TGTCCCCCAA ACCGACCCCA TCACTGGTTT 
GGCAGCAGAG TTTGTCCCTC CTCCGCAGCC GCCCTCTCCC CGCCTCCCGT GACGTGTCCC 
GGGACTCCCC GCCCCGGGCT GGGCTGCGAA GTTGCGGATC CGGTTTGGTC GGGCGGGTCG 
GCGGCTGGAG GAAATACACG GAGCCTCAGG GGCTGCGGCA GCAGCATGGT GGGGCCGGAG 
AAGGAGCAGA GCTGGATCCC TAAGATCTTC AGGAAGAAGG TGTGCACGAC GTTCATAGTG 
GACCTCTCCG ATGATGCGGG AGGGACCTTG TGCCAGTGTG GGCAACCTCG GGATGCCCAC 
CCTTCTGTGG CTGTGGAGGA TGCCTTTGGG GCAGCCGTAG TTACCGAGTG GAACAGTGAT 
GAGCACACCA CGGAGAAGCC CACAGATGCC TATGGGGACC TGGACTTCAC GTACTCTGGC 
CGAAAGCACA GCAACTTTCT CCGGCTGTCT GACCGCACGG ATCCGGCCAC TGTGTACAGT 
CTCGTTACCC GCTCTTGGGG CTTTCGTGCC CCAAACTTGG TGGTGTCGGT GCTGGGGGGG 
TCGGGGGGCC CCGTGCTCCA GACCTGGCTG CAGGACCTTC TGCGTCGCGG GCTGGTGAGA 
GCCGCCCAGA GCACAGGGGC CTGGATCGTC ACTGGGGGAC TGCACACAGG CATTGGCCGG 
CACGTGGGTG TGGCTGTAAG GGACCACCAG ACAGCCAGCA CTGGGAGCAG CAAGGTGGTG 
GCCATGGGTG TGGCCCCCTG GGGAGTGGTC CGGAACAGAG ACATGCTGAT CAACCCCAAG 
GGCTCATTCC CCGCAAGGTA CCGGTGGCGT GGTGACCCTG AGGATGGTGT TGAGTTCCCC 
CTGGACTATA ACTATTCTGC TTTCTTCTTG GTGGATGATG GCACCTATGG CCGCCTGGGT 
GGTGAGAACC GCTTCCGCCT TCGGTTTGAG TCCTATGTGG CTCAGCAGAA GACCGGAGTG 
GGAGGGACTG GAATTGACAT CCCAGTGCTC CTTCTTCTCA TCGATGGAGA TGAGAAGATG 
TTAAAGCGGA TAGAGGATGC CACCCAAGCT CAGCTCCCCT GCCTCTTGGT GGCGGGCTCT 
GGGGGTGCTG CAGACTGTCT GGTGGAGACC CTGGAAGATA CTCTGGCCCC AGGGAGTGGG 
GGACTTAGGC GAGGCGAAGC CCGGGATCGG ATTAGGCGAT ACTTCCCTAA AGGAGACCCT 
GAGGTTCTTC AGGCCCAGGT AGAGAGGATC ATGACCCGAA AGGAGTTGCT GACGGTCTAT 
TCATCAGAAG ACGGCTCCGA GGAGTTTGAG ACTATCGTTT TGAGGGCTCT TGTGAAAGCC 
TGTGGGAGCT CTGAGGCCTC AGCCTACCTG GATGAGCTGC GTTTGGCTGT GGCTTGGAAC 
CGCGTGGACA TCGCCCAAAG TGAACTTTTC CGTGGGGACA TCCAATGGCG GTCCTTCCAC 
CTGGAGGCAT CTCTCATGGA TGCTCTGCTG AATGACCGGC CTGAATTTGT GCGCCTGCTC 
ATCTCCCATG GCCTTAGCCT GGGACACTTC CTGACCCCCG TGCGTCTGGC CCAGCTGTAC 
AGCGCGGTGT CCCCTAACTC ACTGATCCGA AATCTTCTGG ACCAGGCGTC CCATGCTAGT 
AGCAGCAAAT CCCCTCCTGT AAATGGAACT GTAGAGCTCC GGCCTCCCAA TGTGGGGCAA 
GTGCTGAGGA CTCTGTTGGG AGAAACGTGT GCACCACGGT ACCCTGCCAG GAACACCCGA 
GACTCCTATC TGGGCCAGGA CCACAGAGAG AATGACTCTC TGCTTATGGA CTGGGCCAAC 
AAGCAACCAT CAACGGATGC AAGCTTTGAA CAAGCCCCCT GGAGTGACCT GCTTATTTGG 
GCTCTGTTGC TGAACCGGGC CCAGATGGCT ATCTATTTCT GGGAGAAGGG CTCCAACTCA 
GTGGCCTCTG CTCTTGGGGC TTGTCTTTTA CTCCGAGTGA TGGCTCGCCT AGAGTCGGAG 
GCTGAGGAGG CCGCGCGGCG GAAGGATCTT GCTGCCACGT TTGAAAGCAT GAGCGTGGAC 
CTCTTTGGAG AGTGTTACCA CAACAGTGAA GAGCGAGCAG CCCGCCTTCT TCTGCGCCGC 
TGTCCCCTCT GGGGAGAGGC CACCTGCCTC CAGCTTGCCA TGCAGGCTGA TGCCCGAGCG 
TTCTTTGCCC AGGATGGAGT CCAGTCTCTT CTGACACAGA AGTGGTGGGG GGAGATGGAC 
AGCACAACTC CCATCTGGGC CCTGCTTCTC GCCTTCTTCT GCCCGCCTCT CATCTACACC 
AACCTTATCG TCTTCAGGAA GTCAGAGGAG GAGCCCACAC AGAAGGATCT TGACTTTGAT 
ATGGACAGCA GCATCAATGG AGCAGGTCCT CCTGGGACTG TAGAACCCTC TGCAAAGGTG 
GCCCTTGAGA GGCGGCAGCG GCGACGGCCA GGACGTGCCC TCTGCTGTGG CAAGTTCTCC 
AAGCGCTGGT CGGACTTCTG GGGTGCCCCA GTGACTGCCT TCCTGGGTAA TGTGGTCAGT 
TACCTGCTGT TCCTGCTGCT GTTTGCCCAC GTGTTGCTGG TGGATTTCCA GCCCACAAAG 
CCAAGTGTCT CCGAGCTGCT GCTGTACTTC TGGGCCTTCA CGTTGCTGTG CGAGGAGCTG 
CGCCAGGGCC TGGGTGGTGG CTGGGGTAGC CTGGCCAGTG GGGGACGTGG TCCGGACAGA 
GCTCCCCTGC GCCATCGGCT GCACCTCTAC CTCTCAGATA CCTGGAACCA ATGTGACCTG 
CTGGCACTCA CCTGCTTCCT GCTGGGCGTA GGCTGCCGGC TGACCCCTGG GCTCTTTGAC 
TTGGGACGCA CCGTCCTCTG CCTTGACTTC ATGATCTTCA CACTGCGCCT GCTGCACATC 
TTCACGGTGA ACAAGCAGCT GGGGCCCAAG ATTGTCATAG TGAGCAAAAT GCTTATGGGG 
TGGCCACAGA GGGGATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541211.1
CDS	76..3156
Translation	MFVPWLTSIVCPTLLPSFLRLLCLPTLGLSCPPNRPHHWFGSRVCPSSAAALSPPPVTCPGTPRPGLGCEVADPVWSGGSAAGGNTRSLRGCGSSMVGPEKEQSWIPKIFRKKVCTTFIVDLSDDAGGTLCQCGQPRDAHPSVAVEDAFGAAVVTEWNSDEHTTEKPTDAYGDLDFTYSGRKHSNFLRLSDRTDPATVYSLVTRSWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQTASTGSSKVVAMGVAPWGVVRNRDMLINPKGSFPARYRWRGDPEDGVEFPLDYNYSAFFLVDDGTYGRLGGENRFRLRFESYVAQQKTGVGGTGIDIPVLLLLIDGDEKMLKRIEDATQAQLPCLLVAGSGGAADCLVETLEDTLAPGSGGLRRGEARDRIRRYFPKGDPEVLQAQVERIMTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSPPVNGTVELRPPNVGQVLRTLLGETCAPRYPARNTRDSYLGQDHRENDSLLMDWANKQPSTDASFEQAPWSDLLIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLESEAEEAARRKDLAATFESMSVDLFGECYHNSEERAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTTPIWALLLAFFCPPLIYTNLIVFRKSEEEPTQKDLDFDMDSSINGAGPPGTVEPSAKVALERRQRRRPGRALCCGKFSKRWSDFWGAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPSVSELLLYFWAFTLLCEELRQGLGGGWGSLASGGRGPDRAPLRHRLHLYLSDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNKQLGPKIVIVSKMLMGWPQRGS

Target ORF information:

RefSeq Version	XM_006541148.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 4 (Trpm4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541148.3

ORF Insert Sequence:

ATGTTTGTCC CATGGCTGAC ATCTATCGTG TGCCCCACTC TGCTTCCTTC GTTCCTCCGC 
TTGCTCTGCC TTCCAACTCT CGGTCTTTCT TGTCCCCCAA ACCGACCCCA TCACTGGTTT 
GGCAGCAGAG TTTGTCCCTC CTCCGCAGCC GCCCTCTCCC CGCCTCCCGT GACGTGTCCC 
GGGACTCCCC GCCCCGGGCT GGGCTGCGAA GTTGCGGATC CGGTTTGGTC GGGCGGGTCG 
GCGGCTGGAG GAAATACACG GAGCCTCAGG GGCTGCGGCA GCAGCATGGT GGGGCCGGAG 
AAGGAGCAGA GCTGGATCCC TAAGATCTTC AGGAAGAAGG TGTGCACGAC GTTCATAGTG 
GACCTCTCCG ATGATGCGGG AGGGACCTTG TGCCAGTGTG GGCAACCTCG GGATGCCCAC 
CCTTCTGTGG CTGTGGAGGA TGCCTTTGGG GCAGCCGTAG TTACCGAGTG GAACAGTGAT 
GAGCACACCA CGGAGAAGCC CACAGATGCC TATGGGGACC TGGACTTCAC GTACTCTGGC 
CGAAAGCACA GCAACTTTCT CCGGCTGTCT GACCGCACGG ATCCGGCCAC TGTGTACAGT 
CTCGTTACCC GCTCTTGGGG CTTTCGTGCC CCAAACTTGG TGGTGTCGGT GCTGGGGGGG 
TCGGGGGGCC CCGTGCTCCA GACCTGGCTG CAGGACCTTC TGCGTCGCGG GCTGGTGAGA 
GCCGCCCAGA GCACAGGGGC CTGGATCGTC ACTGGGGGAC TGCACACAGG CATTGGCCGG 
CACGTGGGTG TGGCTGTAAG GGACCACCAG ACAGCCAGCA CTGGGAGCAG CAAGGTGGTG 
GCCATGGGTG TGGCCCCCTG GGGAGTGGTC CGGAACAGAG ACATGCTGAT CAACCCCAAG 
GGCTCATTCC CCGCAAGGTA CCGGTGGCGT GGTGACCCTG AGGATGGTGT TGAGTTCCCC 
CTGGACTATA ACTATTCTGC TTTCTTCTTG GTGGATGATG GCACCTATGG CCGCCTGGGT 
GGTGAGAACC GCTTCCGCCT TCGGTTTGAG TCCTATGTGG CTCAGCAGAA GACCGGAGTG 
GGAGGGACTG GAATTGACAT CCCAGTGCTC CTTCTTCTCA TCGATGGAGA TGAGAAGATG 
TTAAAGCGGA TAGAGGATGC CACCCAAGCT CAGCTCCCCT GCCTCTTGGT GGCGGGCTCT 
GGGGGTGCTG CAGACTGTCT GGTGGAGACC CTGGAAGATA CTCTGGCCCC AGGGAGTGGG 
GGACTTAGGC GAGGCGAAGC CCGGGATCGG ATTAGGCGAT ACTTCCCTAA AGGAGACCCT 
GAGGTTCTTC AGGCCCAGGT AGAGAGGATC ATGACCCGAA AGGAGTTGCT GACGGTCTAT 
TCATCAGAAG ACGGCTCCGA GGAGTTTGAG ACTATCGTTT TGAGGGCTCT TGTGAAAGCC 
TGTGGGAGCT CTGAGGCCTC AGCCTACCTG GATGAGCTGC GTTTGGCTGT GGCTTGGAAC 
CGCGTGGACA TCGCCCAAAG TGAACTTTTC CGTGGGGACA TCCAATGGCG GTCCTTCCAC 
CTGGAGGCAT CTCTCATGGA TGCTCTGCTG AATGACCGGC CTGAATTTGT GCGCCTGCTC 
ATCTCCCATG GCCTTAGCCT GGGACACTTC CTGACCCCCG TGCGTCTGGC CCAGCTGTAC 
AGCGCGGTGT CCCCTAACTC ACTGATCCGA AATCTTCTGG ACCAGGCGTC CCATGCTAGT 
AGCAGCAAAT CCCCTCCTGT AAATGGAACT GTAGAGCTCC GGCCTCCCAA TGTGGGGCAA 
GTGCTGAGGA CTCTGTTGGG AGAAACGTGT GCACCACGGT ACCCTGCCAG GAACACCCGA 
GACTCCTATC TGGGCCAGGA CCACAGAGAG AATGACTCTC TGCTTATGGA CTGGGCCAAC 
AAGCAACCAT CAACGGATGC AAGCTTTGAA CAAGCCCCCT GGAGTGACCT GCTTATTTGG 
GCTCTGTTGC TGAACCGGGC CCAGATGGCT ATCTATTTCT GGGAGAAGGG CTCCAACTCA 
GTGGCCTCTG CTCTTGGGGC TTGTCTTTTA CTCCGAGTGA TGGCTCGCCT AGAGTCGGAG 
GCTGAGGAGG CCGCGCGGCG GAAGGATCTT GCTGCCACGT TTGAAAGCAT GAGCGTGGAC 
CTCTTTGGAG AGTGTTACCA CAACAGTGAA GAGCGAGCAG CCCGCCTTCT TCTGCGCCGC 
TGTCCCCTCT GGGGAGAGGC CACCTGCCTC CAGCTTGCCA TGCAGGCTGA TGCCCGAGCG 
TTCTTTGCCC AGGATGGAGT CCAGTCTCTT CTGACACAGA AGTGGTGGGG GGAGATGGAC 
AGCACAACTC CCATCTGGGC CCTGCTTCTC GCCTTCTTCT GCCCGCCTCT CATCTACACC 
AACCTTATCG TCTTCAGGAA GTCAGAGGAG GAGCCCACAC AGAAGGATCT TGACTTTGAT 
ATGGACAGCA GCATCAATGG AGCAGGTCCT CCTGGGACTG TAGAACCCTC TGCAAAGGTG 
GCCCTTGAGA GGCGGCAGCG GCGACGGCCA GGACGTGCCC TCTGCTGTGG CAAGTTCTCC 
AAGCGCTGGT CGGACTTCTG GGGTGCCCCA GTGACTGCCT TCCTGGGTAA TGTGGTCAGT 
TACCTGCTGT TCCTGCTGCT GTTTGCCCAC GTGTTGCTGG TGGATTTCCA GCCCACAAAG 
CCAAGTGTCT CCGAGCTGCT GCTGTACTTC TGGGCCTTCA CGTTGCTGTG CGAGGAGCTG 
CGCCAGGGCC TGGGTGGTGG CTGGGGTAGC CTGGCCAGTG GGGGACGTGG TCCGGACAGA 
GCTCCCCTGC GCCATCGGCT GCACCTCTAC CTCTCAGATA CCTGGAACCA ATGTGACCTG 
CTGGCACTCA CCTGCTTCCT GCTGGGCGTA GGCTGCCGGC TGACCCCTGG GCTCTTTGAC 
TTGGGACGCA CCGTCCTCTG CCTTGACTTC ATGATCTTCA CACTGCGCCT GCTGCACATC 
TTCACGGTGA ACAAGCAGCT GGGGCCCAAG ATTGTCATAG TGAGCAAAAT GCTTATGGGG 
TGGCCACAGA GGGGATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRPM4 and TRPM5 are both required for normal signaling in taste receptor cells.
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)
Dutta Banik D,Martin LE,Freichel M,Torregrossa AM,Medler KF

Structures of the calcium-activated, non-selective cation channel TRPM4.
Nature552(7684)205-209(2017 Dec)
Guo J,She J,Zeng W,Chen Q,Bai XC,Jiang Y

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Trpm4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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