Mtcl1 cDNA ORF clone, Mus musculus(house mouse)

The following Mtcl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mtcl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu53973 XM_006524851.4
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53974 XM_006524852.4
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53975 XM_006524853.4
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu70286 XM_011246587.3
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu79656 XM_017317622.2
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53971 XM_006524849.3
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu23353 NM_172963.4
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu23364 NM_001114098.2
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1392.30
$1989.00
OMu53976 XM_006524856.3
Latest version!
Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
View Old Accession Versions >>
OMu53973 XM_006524851.3 Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu70286 XM_011246587.2 Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu79656 XM_017317622.1 Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu53971 XM_006524849.2 Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OMu53974 XM_006524852.3 Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu53975 XM_006524853.3 Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OMu23364 NM_001114098.1 Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1392.30
$1989.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu53973
    Clone ID Related Accession (Same CDS sequence) XM_006524851.3 , XM_006524851.4
    Accession Version XM_006524851.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524851.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGATAACCC TCCCTCGGGC TATTCTTGCC
    TGGCAGGATG ACAGTGCGGA TTTGAAGTGT CAGCTTCAGT TTGTCAAAGA GGAAGCTTCC
    CTGATGCGCA AAAAGATGGC CAAGTTGGGG CGGGAGAAGG ACGAGTTGGA GCAGGAACTG
    CAGAAGTATA AGTCCCTCTA TGGTGATGTG GACAGTCCCC TCCCAACAGG GGAGGCTGGT
    GGTCCCCCCA GCACCAGGGA GGCAGAGCTG AAACTGCGGC TGAAGTTGGT GGAGGAGGAA
    GCCAGCATCT TGGGCAGGAA GATCGTGGAG CTGGAGGTGG AAAACCGTGG CCTCAAAGCA
    GAGATGGAAG ACATACGGGT TCAGCATGAA CGCGAGGGAA CAGGCAGGGA CCACGTGCCA
    AGCACTCCTA CCTCACCCTT CGGAGACTCA ATGGAGTCCT CCACGGAGCT CCGCCGGCAC
    CTGCAGTTTG TGGAAGAAGA GGCTGAGCTG CTGAGGAGAT CCATCTCTGA GATTGAAGAT
    CACAACCGGC AGTTGACCCA CGAGCTGAGC AAGTTCAAGT TTGAGCCTCA CCAGGAGTCA
    GGGTGGCTTG GGGACGGTGT GTCTAAGGGC CCCGCGGCCA GCGTTCCCCT GCAGGAGGAG
    CTGAAGTCAG CCAGGCTACA GATCGATGAG CTGAGCGGGA AGGTACTGAA GCTGCAGTGT
    GAGAACCGGC TGCTTCTGTC CAATGCCCAA CGGGGTGACC TCGCCGCACA TCTGGGGCTG
    CGCGCTCCTA GCCCCCGGGA CAGTGATGCC GAGAGTGACG CGGGCAAGAA AGAGAGCGAT
    GGGGAGGAGG GCCGCCTTCC CCAGCCAAAG CGAGAAGGCC CTGTTGGTGG GGAGAGCGAC
    TCGGAGGACA TGTTTGAGAA GACCTCGGGC TTCGGAAGCG GAAAGCCGTC GGAGGCTAGT
    GAGCCATGCC CGGCAGAGCT GCTAAGGGTC CGGGAGGATA CCGAGTGCCT GGTGACCATA
    AAGCTGGAGG CTCAGCGGCT TGAGCGGACT GTGGAGCGCC TCATCTCTGA TACAGATGGT
    TTCATCCACG ACTCAGGACT GCGGGGCAAT GGATTGGCCT CACCTGGAGT CCAAGGCGGA
    GGAGGTGAGG GCAACAGCCC GAGTGAGCCC CACTTGTTGG AGACCATCAA TGTGAGGATG
    AAGGCTTTCA GGAAAGAGCT GCAGGCCTTC CTGGAGCAGA TGTCCCGGAT TGTGGATGGT
    CTCTCGCCCT TATCCCACCT CACAGAGTCA TCCAGCTTCC TCTCCACTGT GACTTCTGTG
    TCCCGGGACT CCCCCATCGG GACCCTGGGA AAGGAGTTGG GCCCAGACTT GCAGTCCAAA
    CTGAGAGAGC AGCTGGAATG GCAGCTCAAT CAGGATCGAG GGGACGAGCG AGAGGGCCTG
    CGTCTCCGAG CCACTCGGGA GCTGCACCGG CGCGCGGATG GGGATTCCGG GAGCCACCAT
    GGGCTGGGAG GCCAGAGCTG TTTCAACCTA GAGCTCAGGG GCTCCCCAGT CTTACCTGAG
    CAGAGTGTGT CTGTAGAAGA GCTACAGGGT CAGCTCGAGC AGGCGGCCAG ACTCCATCAA
    GAGGAGACAG AGACATACAC AAACAAGATC CGTAAGATGG AGGAGGACCA CCTCTACGCC
    CTGCGGTGGA AGGAACTGGA GATGCACAGC CTGGCCCTGC AGAACACCCT TCACAAGCGG
    ACGTGGAGCG ACGAGAAGAA TCTGCTGCAG CAGGAGCTCA GGTCCCTGAA GCAGAACATC
    TTCCTCTTCT ACGTCAAGCT CAGGTGGCTG CTGAAACACT GGCGCCAAGG GAAGCAGATG
    GAGGAGGGAG GAGAGGATCT GGAGGAGAGC GAACATCCAG AGAATGTGCC TGGGCTTGCT
    GAGCTTGGAG TCCAGGGGGT TCACCAGACA GATGGGATAG ACCAGGAGGA TGCTGACCAA
    GGCTGTAGCC TTCCCATGGG AGAGCATGCC CCACACTCCC TGGTACAGAT CAGCGAACAC
    GGATCACGGC TACAGAGTTC AGACGGGGGA CCACTTAACA AACAGGTGGT GGAAAACCAG
    CAGCTCTTCA GGGCCCTCAA GGCTCTGCTG GAGGACTTCC GTTCGGAGCT GCGCGAAGAC
    GAGCATGCGC GGCTGCGGCT GCAGCAGCAG TACGCCAGCG ACAAGGCTGC GTGGGATGTG
    GAGTGGGCTG TGCTCAAGTG CCGCCTGGAA CAGCTGGAAG AGAAGACTGA GAAAAGCTTG
    GGCGAGCTGG ACTCCTCTGC TGAGGGCAAA GGGGCCTTGA AGAAGGAGAG AGAGGTGCAC
    CAGAAACTGC TGGCTGACAG CCACAGCCTG GTGATGGACC TGCGCTGGCA GATCCATCAC
    CGAGAGAAGA ACTGGAACCG GGAGAAGGTG GAGCTGCTGG AGCGTCTGGA CAGCGAGCGG
    CAGGAGTGGG GGCGACAGAA GGAGGAGCTC CTGTGGCGGG TGGAGCAGTT ACAGAAAGAG
    AAAAGTCCCC GGAGAAGCGG CAGTTTCCTC TGCAGCCGAA GGGAAGACGA CACACGCCCT
    TACCCGCACC AAGGAAGCCT TCACTCCTCC AGACCTGTGT CCATGTGGCC CTGTGAAGAC
    GCCGACTCCA TCCCATTTGA AGACCGGCCG CTCTCCAAAC TGAAGGAGTC GGACAGGTGC
    TCGGCCAGTG AGAACCTGTA CTTGGACGCT TTGTCCCTGG ATGATGATCC AGGGGATCCG
    CCACCCCTCA GGAACTGCCT TGCCGAGGAA GAAGAAAGTC GCAAGGGCAA TCTTCAGAGG
    GCTGTGTCTG TGTCCTGTAT GTCCGAGTTC CAGCGTCTGA TGGACGTCTC TCCGTTCCTG
    CCTGAGAAGG GGCTACCTTC CGCCGGCAGC AAGGAGGATG TCACTCCACC CTTGTCTCCC
    GACGACTTGA AATACATTGA AGAATTCCAT AGCAACGACT GGGATTACGC ATCCCCCAGG
    GCTGAGGCCG ATCGGCCTCC AGACCCCTGG GCAGACAGGA CCGAGATGGG GAGGGTGGGG
    CATGAAGCCA CCACAGAGCC TTGTCCGGAT CCCTCCTGGT ATCTGACCAC AAGTGTCACG
    ATGACGACAG ACACCATGAC CAGTCCGGAG CACTGCCAGA AGCAGCCACT GCGGACCCAT
    GTCCTCACTG AGCAGTCTGG TGTGCACGTG CTGCACAGCC CGCCTGCCAT TCGCCGGGTT
    GACAGTATAG CATCAGGCGG CGAGGGCAGG AGCCGAGCGG ACCCCGAGGG CCCCTTTCCT
    ATGAGCAGAG CAAGAGGGAA CCTGGCAGAT GCCAAGGGGG GTCATCCAGA ACCTGTGCTC
    AACAGGTGGC CTTGCACGCC ACCCAGACAT CCCCGGGACT GTGTGGAGGG GTCTCTTCGC
    CCCCTCGACA GACCTATCTG CCCCTCCCTG GGATTTGCCT CCCCTCTGAA CAGCCTAGAT
    ATGTCTAAGA ACATGAGTGA TGACATGAAG GAGGTGGCTT TTTCTGTCAG GAATGCCATC
    TGCGCCGGTC CTGCCGAGCC ACACGTCAGG GATATGGCCT GCCAGACCAA TGGGTCCAGG
    ACAGCAGGGA CGCAGACCAT CCAGACAATC AGCGTGGGCC TGCAGACCGA GGCCCTACGT
    GCCAGCGGCG TCACCAGCAG CCCCCACAAG TGTCTCACGC CAAAAGCCGG AGGTGGCACC
    ACACCCGTGT CTTCTCCTTC CCGCAGCCTT AGGAGTAGAC AGGTGGCCCC TGCCATTGAG
    AAGGTGCAGG CCAAGTTTGA ACGCACCTGC TGCTCCCCAA AGTACGGTTC TCCCAAACTT
    CAGAGAAAGC CCCTCTCAAA AGCTGACCAG CCAAATAGCA GGACCTCACC AGGCATACCC
    CAGAAAGGGT TCAGCGAGTC AGCCTGGGCC CGCTCCACCA CCACGAGGGA GAGCCCTGTG
    CACACCACCA TCAATGATGG CCTCTCCAGC CTCTTCAATA TCATCGACCA CAGTCCCATC
    GGGGTGCGGG CTGGGAGCCG ATCTCGGTCA GCAGAACCTC GACAGGAGCT GGGTCCAGGC
    CAGGAAACGG GCACCAGTTC CAGAGGGAGG TCGCCCAGCC CGCTAGGGGT AGGCTCCGAG
    ACGTTCAGGG AGGAGGGAGG AGAGAGCACG CCAGTGAGAC AGGACTTATC TGCGCCCCCT
    GGCTACACGC TCACTGAGAA TGTGGCCCGG ATCCTCAACA AAAAGTTGTT GGAGCATGCC
    TTAAAGGAGG AGAGGAAGCA AGCCAGCCAT GGCTCCTCAG GTCTCACCAG CGACAGCCAC
    ACTGGAGAAC CAGTGCCAGC CGAGCCAGGA TCCATGGAGG AACTGCCTTG TTCTGCGCTC
    GCGCCCTCCC TAGAGCCCTG CTTTTCCAGG CCAGAGAGAC CAGCAAACCG CCGCCTTCCA
    TCCCGTTGGG CCCCACCTTC CCCCACTGCC TCACAGTCAC AGTCACCCGG ACACCCGATG
    TCCATGGAGG AACACGGTGA GGAGGACCCA CCAGAGGAGA AGCCACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524914.1
    CDS460..6453
    Translation

    Target ORF information:

    RefSeq Version XM_006524851.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524851.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGATAACCC TCCCTCGGGC TATTCTTGCC
    TGGCAGGATG ACAGTGCGGA TTTGAAGTGT CAGCTTCAGT TTGTCAAAGA GGAAGCTTCC
    CTGATGCGCA AAAAGATGGC CAAGTTGGGG CGGGAGAAGG ACGAGTTGGA GCAGGAACTG
    CAGAAGTATA AGTCCCTCTA TGGTGATGTG GACAGTCCCC TCCCAACAGG GGAGGCTGGT
    GGTCCCCCCA GCACCAGGGA GGCAGAGCTG AAACTGCGGC TGAAGTTGGT GGAGGAGGAA
    GCCAGCATCT TGGGCAGGAA GATCGTGGAG CTGGAGGTGG AAAACCGTGG CCTCAAAGCA
    GAGATGGAAG ACATACGGGT TCAGCATGAA CGCGAGGGAA CAGGCAGGGA CCACGTGCCA
    AGCACTCCTA CCTCACCCTT CGGAGACTCA ATGGAGTCCT CCACGGAGCT CCGCCGGCAC
    CTGCAGTTTG TGGAAGAAGA GGCTGAGCTG CTGAGGAGAT CCATCTCTGA GATTGAAGAT
    CACAACCGGC AGTTGACCCA CGAGCTGAGC AAGTTCAAGT TTGAGCCTCA CCAGGAGTCA
    GGGTGGCTTG GGGACGGTGT GTCTAAGGGC CCCGCGGCCA GCGTTCCCCT GCAGGAGGAG
    CTGAAGTCAG CCAGGCTACA GATCGATGAG CTGAGCGGGA AGGTACTGAA GCTGCAGTGT
    GAGAACCGGC TGCTTCTGTC CAATGCCCAA CGGGGTGACC TCGCCGCACA TCTGGGGCTG
    CGCGCTCCTA GCCCCCGGGA CAGTGATGCC GAGAGTGACG CGGGCAAGAA AGAGAGCGAT
    GGGGAGGAGG GCCGCCTTCC CCAGCCAAAG CGAGAAGGCC CTGTTGGTGG GGAGAGCGAC
    TCGGAGGACA TGTTTGAGAA GACCTCGGGC TTCGGAAGCG GAAAGCCGTC GGAGGCTAGT
    GAGCCATGCC CGGCAGAGCT GCTAAGGGTC CGGGAGGATA CCGAGTGCCT GGTGACCATA
    AAGCTGGAGG CTCAGCGGCT TGAGCGGACT GTGGAGCGCC TCATCTCTGA TACAGATGGT
    TTCATCCACG ACTCAGGACT GCGGGGCAAT GGATTGGCCT CACCTGGAGT CCAAGGCGGA
    GGAGGTGAGG GCAACAGCCC GAGTGAGCCC CACTTGTTGG AGACCATCAA TGTGAGGATG
    AAGGCTTTCA GGAAAGAGCT GCAGGCCTTC CTGGAGCAGA TGTCCCGGAT TGTGGATGGT
    CTCTCGCCCT TATCCCACCT CACAGAGTCA TCCAGCTTCC TCTCCACTGT GACTTCTGTG
    TCCCGGGACT CCCCCATCGG GACCCTGGGA AAGGAGTTGG GCCCAGACTT GCAGTCCAAA
    CTGAGAGAGC AGCTGGAATG GCAGCTCAAT CAGGATCGAG GGGACGAGCG AGAGGGCCTG
    CGTCTCCGAG CCACTCGGGA GCTGCACCGG CGCGCGGATG GGGATTCCGG GAGCCACCAT
    GGGCTGGGAG GCCAGAGCTG TTTCAACCTA GAGCTCAGGG GCTCCCCAGT CTTACCTGAG
    CAGAGTGTGT CTGTAGAAGA GCTACAGGGT CAGCTCGAGC AGGCGGCCAG ACTCCATCAA
    GAGGAGACAG AGACATACAC AAACAAGATC CGTAAGATGG AGGAGGACCA CCTCTACGCC
    CTGCGGTGGA AGGAACTGGA GATGCACAGC CTGGCCCTGC AGAACACCCT TCACAAGCGG
    ACGTGGAGCG ACGAGAAGAA TCTGCTGCAG CAGGAGCTCA GGTCCCTGAA GCAGAACATC
    TTCCTCTTCT ACGTCAAGCT CAGGTGGCTG CTGAAACACT GGCGCCAAGG GAAGCAGATG
    GAGGAGGGAG GAGAGGATCT GGAGGAGAGC GAACATCCAG AGAATGTGCC TGGGCTTGCT
    GAGCTTGGAG TCCAGGGGGT TCACCAGACA GATGGGATAG ACCAGGAGGA TGCTGACCAA
    GGCTGTAGCC TTCCCATGGG AGAGCATGCC CCACACTCCC TGGTACAGAT CAGCGAACAC
    GGATCACGGC TACAGAGTTC AGACGGGGGA CCACTTAACA AACAGGTGGT GGAAAACCAG
    CAGCTCTTCA GGGCCCTCAA GGCTCTGCTG GAGGACTTCC GTTCGGAGCT GCGCGAAGAC
    GAGCATGCGC GGCTGCGGCT GCAGCAGCAG TACGCCAGCG ACAAGGCTGC GTGGGATGTG
    GAGTGGGCTG TGCTCAAGTG CCGCCTGGAA CAGCTGGAAG AGAAGACTGA GAAAAGCTTG
    GGCGAGCTGG ACTCCTCTGC TGAGGGCAAA GGGGCCTTGA AGAAGGAGAG AGAGGTGCAC
    CAGAAACTGC TGGCTGACAG CCACAGCCTG GTGATGGACC TGCGCTGGCA GATCCATCAC
    CGAGAGAAGA ACTGGAACCG GGAGAAGGTG GAGCTGCTGG AGCGTCTGGA CAGCGAGCGG
    CAGGAGTGGG GGCGACAGAA GGAGGAGCTC CTGTGGCGGG TGGAGCAGTT ACAGAAAGAG
    AAAAGTCCCC GGAGAAGCGG CAGTTTCCTC TGCAGCCGAA GGGAAGACGA CACACGCCCT
    TACCCGCACC AAGGAAGCCT TCACTCCTCC AGACCTGTGT CCATGTGGCC CTGTGAAGAC
    GCCGACTCCA TCCCATTTGA AGACCGGCCG CTCTCCAAAC TGAAGGAGTC GGACAGGTGC
    TCGGCCAGTG AGAACCTGTA CTTGGACGCT TTGTCCCTGG ATGATGATCC AGGGGATCCG
    CCACCCCTCA GGAACTGCCT TGCCGAGGAA GAAGAAAGTC GCAAGGGCAA TCTTCAGAGG
    GCTGTGTCTG TGTCCTGTAT GTCCGAGTTC CAGCGTCTGA TGGACGTCTC TCCGTTCCTG
    CCTGAGAAGG GGCTACCTTC CGCCGGCAGC AAGGAGGATG TCACTCCACC CTTGTCTCCC
    GACGACTTGA AATACATTGA AGAATTCCAT AGCAACGACT GGGATTACGC ATCCCCCAGG
    GCTGAGGCCG ATCGGCCTCC AGACCCCTGG GCAGACAGGA CCGAGATGGG GAGGGTGGGG
    CATGAAGCCA CCACAGAGCC TTGTCCGGAT CCCTCCTGGT ATCTGACCAC AAGTGTCACG
    ATGACGACAG ACACCATGAC CAGTCCGGAG CACTGCCAGA AGCAGCCACT GCGGACCCAT
    GTCCTCACTG AGCAGTCTGG TGTGCACGTG CTGCACAGCC CGCCTGCCAT TCGCCGGGTT
    GACAGTATAG CATCAGGCGG CGAGGGCAGG AGCCGAGCGG ACCCCGAGGG CCCCTTTCCT
    ATGAGCAGAG CAAGAGGGAA CCTGGCAGAT GCCAAGGGGG GTCATCCAGA ACCTGTGCTC
    AACAGGTGGC CTTGCACGCC ACCCAGACAT CCCCGGGACT GTGTGGAGGG GTCTCTTCGC
    CCCCTCGACA GACCTATCTG CCCCTCCCTG GGATTTGCCT CCCCTCTGAA CAGCCTAGAT
    ATGTCTAAGA ACATGAGTGA TGACATGAAG GAGGTGGCTT TTTCTGTCAG GAATGCCATC
    TGCGCCGGTC CTGCCGAGCC ACACGTCAGG GATATGGCCT GCCAGACCAA TGGGTCCAGG
    ACAGCAGGGA CGCAGACCAT CCAGACAATC AGCGTGGGCC TGCAGACCGA GGCCCTACGT
    GCCAGCGGCG TCACCAGCAG CCCCCACAAG TGTCTCACGC CAAAAGCCGG AGGTGGCACC
    ACACCCGTGT CTTCTCCTTC CCGCAGCCTT AGGAGTAGAC AGGTGGCCCC TGCCATTGAG
    AAGGTGCAGG CCAAGTTTGA ACGCACCTGC TGCTCCCCAA AGTACGGTTC TCCCAAACTT
    CAGAGAAAGC CCCTCTCAAA AGCTGACCAG CCAAATAGCA GGACCTCACC AGGCATACCC
    CAGAAAGGGT TCAGCGAGTC AGCCTGGGCC CGCTCCACCA CCACGAGGGA GAGCCCTGTG
    CACACCACCA TCAATGATGG CCTCTCCAGC CTCTTCAATA TCATCGACCA CAGTCCCATC
    GGGGTGCGGG CTGGGAGCCG ATCTCGGTCA GCAGAACCTC GACAGGAGCT GGGTCCAGGC
    CAGGAAACGG GCACCAGTTC CAGAGGGAGG TCGCCCAGCC CGCTAGGGGT AGGCTCCGAG
    ACGTTCAGGG AGGAGGGAGG AGAGAGCACG CCAGTGAGAC AGGACTTATC TGCGCCCCCT
    GGCTACACGC TCACTGAGAA TGTGGCCCGG ATCCTCAACA AAAAGTTGTT GGAGCATGCC
    TTAAAGGAGG AGAGGAAGCA AGCCAGCCAT GGCTCCTCAG GTCTCACCAG CGACAGCCAC
    ACTGGAGAAC CAGTGCCAGC CGAGCCAGGA TCCATGGAGG AACTGCCTTG TTCTGCGCTC
    GCGCCCTCCC TAGAGCCCTG CTTTTCCAGG CCAGAGAGAC CAGCAAACCG CCGCCTTCCA
    TCCCGTTGGG CCCCACCTTC CCCCACTGCC TCACAGTCAC AGTCACCCGG ACACCCGATG
    TCCATGGAGG AACACGGTGA GGAGGACCCA CCAGAGGAGA AGCCACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53974
    Clone ID Related Accession (Same CDS sequence) XM_006524852.3 , XM_006524852.4
    Accession Version XM_006524852.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524852.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    CTCAGGGGCT CCCCAGTCTT ACCTGAGCAG AGTGTGTCTG TAGAAGAGCT ACAGGGTCAG
    CTCGAGCAGG CGGCCAGACT CCATCAAGAG GAGACAGAGA CATACACAAA CAAGATCCGT
    AAGATGGAGG AGGACCACCT CTACGCCCTG CGGTGGAAGG AACTGGAGAT GCACAGCCTG
    GCCCTGCAGA ACACCCTTCA CAAGCGGACG TGGAGCGACG AGAAGAATCT GCTGCAGCAG
    GAGCTCAGGT CCCTGAAGCA GAACATCTTC CTCTTCTACG TCAAGCTCAG GTGGCTGCTG
    AAACACTGGC GCCAAGGGAA GCAGATGGAG GAGGGAGGAG AGGATCTGGA GGAGAGCGAA
    CATCCAGAGA ATGTGCCTGG GCTTGCTGAG CTTGGAGTCC AGGGGGTTCA CCAGACAGAT
    GGGATAGACC AGGAGGATGC TGACCAAGGC TGTAGCCTTC CCATGGGAGA GCATGCCCCA
    CACTCCCTGG TACAGATCAG CGAACACGGA TCACGGCTAC AGAGTTCAGA CGGGGGACCA
    CTTAACAAAC AGGTGGTGGA AAACCAGCAG CTCTTCAGGG CCCTCAAGGC TCTGCTGGAG
    GACTTCCGTT CGGAGCTGCG CGAAGACGAG CATGCGCGGC TGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AGGCTGCGTG GGATGTGGAG TGGGCTGTGC TCAAGTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA AAGCTTGGGC GAGCTGGACT CCTCTGCTGA GGGCAAAGGG
    GCCTTGAAGA AGGAGAGAGA GGTGCACCAG AAACTGCTGG CTGACAGCCA CAGCCTGGTG
    ATGGACCTGC GCTGGCAGAT CCATCACCGA GAGAAGAACT GGAACCGGGA GAAGGTGGAG
    CTGCTGGAGC GTCTGGACAG CGAGCGGCAG GAGTGGGGGC GACAGAAGGA GGAGCTCCTG
    TGGCGGGTGG AGCAGTTACA GAAAGAGAAA AGTCCCCGGA GAAGCGGCAG TTTCCTCTGC
    AGCCGAAGGG AAGACGACAC ACGCCCTTAC CCGCACCAAG GAAGCCTTCA CTCCTCCAGA
    CCTGTGTCCA TGTGGCCCTG TGAAGACGCC GACTCCATCC CATTTGAAGA CCGGCCGCTC
    TCCAAACTGA AGGAGTCGGA CAGGTGCTCG GCCAGTGAGA ACCTGTACTT GGACGCTTTG
    TCCCTGGATG ATGATCCAGG GGATCCGCCA CCCCTCAGGA ACTGCCTTGC CGAGGAAGAA
    GAAAGTCGCA AGGGCAATCT TCAGAGGGCT GTGTCTGTGT CCTGTATGTC CGAGTTCCAG
    CGTCTGATGG ACGTCTCTCC GTTCCTGCCT GAGAAGGGGC TACCTTCCGC CGGCAGCAAG
    GAGGATGTCA CTCCACCCTT GTCTCCCGAC GACTTGAAAT ACATTGAAGA ATTCCATAGC
    AACGACTGGG ATTACGCATC CCCCAGGGCT GAGGCCGATC GGCCTCCAGA CCCCTGGGCA
    GACAGGACCG AGATGGGGAG GGTGGGGCAT GAAGCCACCA CAGAGCCTTG TCCGGATCCC
    TCCTGGTATC TGACCACAAG TGTCACGATG ACGACAGACA CCATGACCAG TCCGGAGCAC
    TGCCAGAAGC AGCCACTGCG GACCCATGTC CTCACTGAGC AGTCTGGTGT GCACGTGCTG
    CACAGCCCGC CTGCCATTCG CCGGGTTGAC AGTATAGCAT CAGGCGGCGA GGGCAGGAGC
    CGAGCGGACC CCGAGGGCCC CTTTCCTATG AGCAGAGCAA GAGGGAACCT GGCAGATGCC
    AAGGGGGGTC ATCCAGAACC TGTGCTCAAC AGGTGGCCTT GCACGCCACC CAGACATCCC
    CGGGACTGTG TGGAGGGGTC TCTTCGCCCC CTCGACAGAC CTATCTGCCC CTCCCTGGGA
    TTTGCCTCCC CTCTGAACAG CCTAGATATG TCTAAGAACA TGAGTGATGA CATGAAGGAG
    GTGGCTTTTT CTGTCAGGAA TGCCATCTGC GCCGGTCCTG CCGAGCCACA CGTCAGGGAT
    ATGGCCTGCC AGACCAATGG GTCCAGGACA GCAGGGACGC AGACCATCCA GACAATCAGC
    GTGGGCCTGC AGACCGAGGC CCTACGTGCC AGCGGCGTCA CCAGCAGCCC CCACAAGTGT
    CTCACGCCAA AAGCCGGAGG TGGCACCACA CCCGTGTCTT CTCCTTCCCG CAGCCTTAGG
    AGTAGACAGG TGGCCCCTGC CATTGAGAAG GTGCAGGCCA AGTTTGAACG CACCTGCTGC
    TCCCCAAAGT ACGGTTCTCC CAAACTTCAG AGAAAGCCCC TCTCAAAAGC TGACCAGCCA
    AATAGCAGGA CCTCACCAGG CATACCCCAG AAAGGGTTCA GCGAGTCAGC CTGGGCCCGC
    TCCACCACCA CGAGGGAGAG CCCTGTGCAC ACCACCATCA ATGATGGCCT CTCCAGCCTC
    TTCAATATCA TCGACCACAG TCCCATCGGG GTGCGGGCTG GGAGCCGATC TCGGTCAGCA
    GAACCTCGAC AGGAGCTGGG TCCAGGCCAG GAAACGGGCA CCAGTTCCAG AGGGAGGTCG
    CCCAGCCCGC TAGGGGTAGG CTCCGAGACG TTCAGGGAGG AGGGAGGAGA GAGCACGCCA
    GTGAGACAGG ACTTATCTGC GCCCCCTGGC TACACGCTCA CTGAGAATGT GGCCCGGATC
    CTCAACAAAA AGTTGTTGGA GCATGCCTTA AAGGAGGAGA GGAAGCAAGC CAGCCATGGC
    TCCTCAGGTC TCACCAGCGA CAGCCACACT GGAGAACCAG TGCCAGCCGA GCCAGGATCC
    ATGGAGGAAC TGCCTTGTTC TGCGCTCGCG CCCTCCCTAG AGCCCTGCTT TTCCAGGCCA
    GAGAGACCAG CAAACCGCCG CCTTCCATCC CGTTGGGCCC CACCTTCCCC CACTGCCTCA
    CAGTCACAGT CACCCGGACA CCCGATGTCC ATGGAGGAAC ACGGTGAGGA GGACCCACCA
    GAGGAGAAGC CACACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524915.1
    CDS460..6420
    Translation

    Target ORF information:

    RefSeq Version XM_006524852.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524852.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    CTCAGGGGCT CCCCAGTCTT ACCTGAGCAG AGTGTGTCTG TAGAAGAGCT ACAGGGTCAG
    CTCGAGCAGG CGGCCAGACT CCATCAAGAG GAGACAGAGA CATACACAAA CAAGATCCGT
    AAGATGGAGG AGGACCACCT CTACGCCCTG CGGTGGAAGG AACTGGAGAT GCACAGCCTG
    GCCCTGCAGA ACACCCTTCA CAAGCGGACG TGGAGCGACG AGAAGAATCT GCTGCAGCAG
    GAGCTCAGGT CCCTGAAGCA GAACATCTTC CTCTTCTACG TCAAGCTCAG GTGGCTGCTG
    AAACACTGGC GCCAAGGGAA GCAGATGGAG GAGGGAGGAG AGGATCTGGA GGAGAGCGAA
    CATCCAGAGA ATGTGCCTGG GCTTGCTGAG CTTGGAGTCC AGGGGGTTCA CCAGACAGAT
    GGGATAGACC AGGAGGATGC TGACCAAGGC TGTAGCCTTC CCATGGGAGA GCATGCCCCA
    CACTCCCTGG TACAGATCAG CGAACACGGA TCACGGCTAC AGAGTTCAGA CGGGGGACCA
    CTTAACAAAC AGGTGGTGGA AAACCAGCAG CTCTTCAGGG CCCTCAAGGC TCTGCTGGAG
    GACTTCCGTT CGGAGCTGCG CGAAGACGAG CATGCGCGGC TGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AGGCTGCGTG GGATGTGGAG TGGGCTGTGC TCAAGTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA AAGCTTGGGC GAGCTGGACT CCTCTGCTGA GGGCAAAGGG
    GCCTTGAAGA AGGAGAGAGA GGTGCACCAG AAACTGCTGG CTGACAGCCA CAGCCTGGTG
    ATGGACCTGC GCTGGCAGAT CCATCACCGA GAGAAGAACT GGAACCGGGA GAAGGTGGAG
    CTGCTGGAGC GTCTGGACAG CGAGCGGCAG GAGTGGGGGC GACAGAAGGA GGAGCTCCTG
    TGGCGGGTGG AGCAGTTACA GAAAGAGAAA AGTCCCCGGA GAAGCGGCAG TTTCCTCTGC
    AGCCGAAGGG AAGACGACAC ACGCCCTTAC CCGCACCAAG GAAGCCTTCA CTCCTCCAGA
    CCTGTGTCCA TGTGGCCCTG TGAAGACGCC GACTCCATCC CATTTGAAGA CCGGCCGCTC
    TCCAAACTGA AGGAGTCGGA CAGGTGCTCG GCCAGTGAGA ACCTGTACTT GGACGCTTTG
    TCCCTGGATG ATGATCCAGG GGATCCGCCA CCCCTCAGGA ACTGCCTTGC CGAGGAAGAA
    GAAAGTCGCA AGGGCAATCT TCAGAGGGCT GTGTCTGTGT CCTGTATGTC CGAGTTCCAG
    CGTCTGATGG ACGTCTCTCC GTTCCTGCCT GAGAAGGGGC TACCTTCCGC CGGCAGCAAG
    GAGGATGTCA CTCCACCCTT GTCTCCCGAC GACTTGAAAT ACATTGAAGA ATTCCATAGC
    AACGACTGGG ATTACGCATC CCCCAGGGCT GAGGCCGATC GGCCTCCAGA CCCCTGGGCA
    GACAGGACCG AGATGGGGAG GGTGGGGCAT GAAGCCACCA CAGAGCCTTG TCCGGATCCC
    TCCTGGTATC TGACCACAAG TGTCACGATG ACGACAGACA CCATGACCAG TCCGGAGCAC
    TGCCAGAAGC AGCCACTGCG GACCCATGTC CTCACTGAGC AGTCTGGTGT GCACGTGCTG
    CACAGCCCGC CTGCCATTCG CCGGGTTGAC AGTATAGCAT CAGGCGGCGA GGGCAGGAGC
    CGAGCGGACC CCGAGGGCCC CTTTCCTATG AGCAGAGCAA GAGGGAACCT GGCAGATGCC
    AAGGGGGGTC ATCCAGAACC TGTGCTCAAC AGGTGGCCTT GCACGCCACC CAGACATCCC
    CGGGACTGTG TGGAGGGGTC TCTTCGCCCC CTCGACAGAC CTATCTGCCC CTCCCTGGGA
    TTTGCCTCCC CTCTGAACAG CCTAGATATG TCTAAGAACA TGAGTGATGA CATGAAGGAG
    GTGGCTTTTT CTGTCAGGAA TGCCATCTGC GCCGGTCCTG CCGAGCCACA CGTCAGGGAT
    ATGGCCTGCC AGACCAATGG GTCCAGGACA GCAGGGACGC AGACCATCCA GACAATCAGC
    GTGGGCCTGC AGACCGAGGC CCTACGTGCC AGCGGCGTCA CCAGCAGCCC CCACAAGTGT
    CTCACGCCAA AAGCCGGAGG TGGCACCACA CCCGTGTCTT CTCCTTCCCG CAGCCTTAGG
    AGTAGACAGG TGGCCCCTGC CATTGAGAAG GTGCAGGCCA AGTTTGAACG CACCTGCTGC
    TCCCCAAAGT ACGGTTCTCC CAAACTTCAG AGAAAGCCCC TCTCAAAAGC TGACCAGCCA
    AATAGCAGGA CCTCACCAGG CATACCCCAG AAAGGGTTCA GCGAGTCAGC CTGGGCCCGC
    TCCACCACCA CGAGGGAGAG CCCTGTGCAC ACCACCATCA ATGATGGCCT CTCCAGCCTC
    TTCAATATCA TCGACCACAG TCCCATCGGG GTGCGGGCTG GGAGCCGATC TCGGTCAGCA
    GAACCTCGAC AGGAGCTGGG TCCAGGCCAG GAAACGGGCA CCAGTTCCAG AGGGAGGTCG
    CCCAGCCCGC TAGGGGTAGG CTCCGAGACG TTCAGGGAGG AGGGAGGAGA GAGCACGCCA
    GTGAGACAGG ACTTATCTGC GCCCCCTGGC TACACGCTCA CTGAGAATGT GGCCCGGATC
    CTCAACAAAA AGTTGTTGGA GCATGCCTTA AAGGAGGAGA GGAAGCAAGC CAGCCATGGC
    TCCTCAGGTC TCACCAGCGA CAGCCACACT GGAGAACCAG TGCCAGCCGA GCCAGGATCC
    ATGGAGGAAC TGCCTTGTTC TGCGCTCGCG CCCTCCCTAG AGCCCTGCTT TTCCAGGCCA
    GAGAGACCAG CAAACCGCCG CCTTCCATCC CGTTGGGCCC CACCTTCCCC CACTGCCTCA
    CAGTCACAGT CACCCGGACA CCCGATGTCC ATGGAGGAAC ACGGTGAGGA GGACCCACCA
    GAGGAGAAGC CACACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53975
    Clone ID Related Accession (Same CDS sequence) XM_006524853.3 , XM_006524853.4
    Accession Version XM_006524853.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524853.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGAT GGAGGAGGAC
    CACCTCTACG CCCTGCGGTG GAAGGAACTG GAGATGCACA GCCTGGCCCT GCAGAACACC
    CTTCACAAGC GGACGTGGAG CGACGAGAAG AATCTGCTGC AGCAGGAGCT CAGGTCCCTG
    AAGCAGAACA TCTTCCTCTT CTACGTCAAG CTCAGGTGGC TGCTGAAACA CTGGCGCCAA
    GGGAAGCAGA TGGAGGAGGG AGGAGAGGAT CTGGAGGAGA GCGAACATCC AGAGAATGTG
    CCTGGGCTTG CTGAGCTTGG AGTCCAGGGG GTTCACCAGA CAGATGGGAT AGACCAGGAG
    GATGCTGACC AAGGCTGTAG CCTTCCCATG GGAGAGCATG CCCCACACTC CCTGGTACAG
    ATCAGCGAAC ACGGATCACG GCTACAGAGT TCAGACGGGG GACCACTTAA CAAACAGGTG
    GTGGAAAACC AGCAGCTCTT CAGGGCCCTC AAGGCTCTGC TGGAGGACTT CCGTTCGGAG
    CTGCGCGAAG ACGAGCATGC GCGGCTGCGG CTGCAGCAGC AGTACGCCAG CGACAAGGCT
    GCGTGGGATG TGGAGTGGGC TGTGCTCAAG TGCCGCCTGG AACAGCTGGA AGAGAAGACT
    GAGAAAAGCT TGGGCGAGCT GGACTCCTCT GCTGAGGGCA AAGGGGCCTT GAAGAAGGAG
    AGAGAGGTGC ACCAGAAACT GCTGGCTGAC AGCCACAGCC TGGTGATGGA CCTGCGCTGG
    CAGATCCATC ACCGAGAGAA GAACTGGAAC CGGGAGAAGG TGGAGCTGCT GGAGCGTCTG
    GACAGCGAGC GGCAGGAGTG GGGGCGACAG AAGGAGGAGC TCCTGTGGCG GGTGGAGCAG
    TTACAGAAAG AGAAAAGTCC CCGGAGAAGC GGCAGTTTCC TCTGCAGCCG AAGGGAAGAC
    GACACACGCC CTTACCCGCA CCAAGGAAGC CTTCACTCCT CCAGACCTGT GTCCATGTGG
    CCCTGTGAAG ACGCCGACTC CATCCCATTT GAAGACCGGC CGCTCTCCAA ACTGAAGGAG
    TCGGACAGGT GCTCGGCCAG TGAGAACCTG TACTTGGACG CTTTGTCCCT GGATGATGAT
    CCAGGGGATC CGCCACCCCT CAGGAACTGC CTTGCCGAGG AAGAAGAAAG TCGCAAGGGC
    AATCTTCAGA GGGCTGTGTC TGTGTCCTGT ATGTCCGAGT TCCAGCGTCT GATGGACGTC
    TCTCCGTTCC TGCCTGAGAA GGGGCTACCT TCCGCCGGCA GCAAGGAGGA TGTCACTCCA
    CCCTTGTCTC CCGACGACTT GAAATACATT GAAGAATTCC ATAGCAACGA CTGGGATTAC
    GCATCCCCCA GGGCTGAGGC CGATCGGCCT CCAGACCCCT GGGCAGACAG GACCGAGATG
    GGGAGGGTGG GGCATGAAGC CACCACAGAG CCTTGTCCGG ATCCCTCCTG GTATCTGACC
    ACAAGTGTCA CGATGACGAC AGACACCATG ACCAGTCCGG AGCACTGCCA GAAGCAGCCA
    CTGCGGACCC ATGTCCTCAC TGAGCAGTCT GGTGTGCACG TGCTGCACAG CCCGCCTGCC
    ATTCGCCGGG TTGACAGTAT AGCATCAGGC GGCGAGGGCA GGAGCCGAGC GGACCCCGAG
    GGCCCCTTTC CTATGAGCAG AGCAAGAGGG AACCTGGCAG ATGCCAAGGG GGGTCATCCA
    GAACCTGTGC TCAACAGGTG GCCTTGCACG CCACCCAGAC ATCCCCGGGA CTGTGTGGAG
    GGGTCTCTTC GCCCCCTCGA CAGACCTATC TGCCCCTCCC TGGGATTTGC CTCCCCTCTG
    AACAGCCTAG ATATGTCTAA GAACATGAGT GATGACATGA AGGAGGTGGC TTTTTCTGTC
    AGGAATGCCA TCTGCGCCGG TCCTGCCGAG CCACACGTCA GGGATATGGC CTGCCAGACC
    AATGGGTCCA GGACAGCAGG GACGCAGACC ATCCAGACAA TCAGCGTGGG CCTGCAGACC
    GAGGCCCTAC GTGCCAGCGG CGTCACCAGC AGCCCCCACA AGTGTCTCAC GCCAAAAGCC
    GGAGGTGGCA CCACACCCGT GTCTTCTCCT TCCCGCAGCC TTAGGAGTAG ACAGGTGGCC
    CCTGCCATTG AGAAGGTGCA GGCCAAGTTT GAACGCACCT GCTGCTCCCC AAAGTACGGT
    TCTCCCAAAC TTCAGAGAAA GCCCCTCTCA AAAGCTGACC AGCCAAATAG CAGGACCTCA
    CCAGGCATAC CCCAGAAAGG GTTCAGCGAG TCAGCCTGGG CCCGCTCCAC CACCACGAGG
    GAGAGCCCTG TGCACACCAC CATCAATGAT GGCCTCTCCA GCCTCTTCAA TATCATCGAC
    CACAGTCCCA TCGGGGTGCG GGCTGGGAGC CGATCTCGGT CAGCAGAACC TCGACAGGAG
    CTGGGTCCAG GCCAGGAAAC GGGCACCAGT TCCAGAGGGA GGTCGCCCAG CCCGCTAGGG
    GTAGGCTCCG AGACGTTCAG GGAGGAGGGA GGAGAGAGCA CGCCAGTGAG ACAGGACTTA
    TCTGCGCCCC CTGGCTACAC GCTCACTGAG AATGTGGCCC GGATCCTCAA CAAAAAGTTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAAG CAAGCCAGCC ATGGCTCCTC AGGTCTCACC
    AGCGACAGCC ACACTGGAGA ACCAGTGCCA GCCGAGCCAG GATCCATGGA GGAACTGCCT
    TGTTCTGCGC TCGCGCCCTC CCTAGAGCCC TGCTTTTCCA GGCCAGAGAG ACCAGCAAAC
    CGCCGCCTTC CATCCCGTTG GGCCCCACCT TCCCCCACTG CCTCACAGTC ACAGTCACCC
    GGACACCCGA TGTCCATGGA GGAACACGGT GAGGAGGACC CACCAGAGGA GAAGCCACAC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524916.1
    CDS460..6405
    Translation

    Target ORF information:

    RefSeq Version XM_006524853.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524853.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGAT GGAGGAGGAC
    CACCTCTACG CCCTGCGGTG GAAGGAACTG GAGATGCACA GCCTGGCCCT GCAGAACACC
    CTTCACAAGC GGACGTGGAG CGACGAGAAG AATCTGCTGC AGCAGGAGCT CAGGTCCCTG
    AAGCAGAACA TCTTCCTCTT CTACGTCAAG CTCAGGTGGC TGCTGAAACA CTGGCGCCAA
    GGGAAGCAGA TGGAGGAGGG AGGAGAGGAT CTGGAGGAGA GCGAACATCC AGAGAATGTG
    CCTGGGCTTG CTGAGCTTGG AGTCCAGGGG GTTCACCAGA CAGATGGGAT AGACCAGGAG
    GATGCTGACC AAGGCTGTAG CCTTCCCATG GGAGAGCATG CCCCACACTC CCTGGTACAG
    ATCAGCGAAC ACGGATCACG GCTACAGAGT TCAGACGGGG GACCACTTAA CAAACAGGTG
    GTGGAAAACC AGCAGCTCTT CAGGGCCCTC AAGGCTCTGC TGGAGGACTT CCGTTCGGAG
    CTGCGCGAAG ACGAGCATGC GCGGCTGCGG CTGCAGCAGC AGTACGCCAG CGACAAGGCT
    GCGTGGGATG TGGAGTGGGC TGTGCTCAAG TGCCGCCTGG AACAGCTGGA AGAGAAGACT
    GAGAAAAGCT TGGGCGAGCT GGACTCCTCT GCTGAGGGCA AAGGGGCCTT GAAGAAGGAG
    AGAGAGGTGC ACCAGAAACT GCTGGCTGAC AGCCACAGCC TGGTGATGGA CCTGCGCTGG
    CAGATCCATC ACCGAGAGAA GAACTGGAAC CGGGAGAAGG TGGAGCTGCT GGAGCGTCTG
    GACAGCGAGC GGCAGGAGTG GGGGCGACAG AAGGAGGAGC TCCTGTGGCG GGTGGAGCAG
    TTACAGAAAG AGAAAAGTCC CCGGAGAAGC GGCAGTTTCC TCTGCAGCCG AAGGGAAGAC
    GACACACGCC CTTACCCGCA CCAAGGAAGC CTTCACTCCT CCAGACCTGT GTCCATGTGG
    CCCTGTGAAG ACGCCGACTC CATCCCATTT GAAGACCGGC CGCTCTCCAA ACTGAAGGAG
    TCGGACAGGT GCTCGGCCAG TGAGAACCTG TACTTGGACG CTTTGTCCCT GGATGATGAT
    CCAGGGGATC CGCCACCCCT CAGGAACTGC CTTGCCGAGG AAGAAGAAAG TCGCAAGGGC
    AATCTTCAGA GGGCTGTGTC TGTGTCCTGT ATGTCCGAGT TCCAGCGTCT GATGGACGTC
    TCTCCGTTCC TGCCTGAGAA GGGGCTACCT TCCGCCGGCA GCAAGGAGGA TGTCACTCCA
    CCCTTGTCTC CCGACGACTT GAAATACATT GAAGAATTCC ATAGCAACGA CTGGGATTAC
    GCATCCCCCA GGGCTGAGGC CGATCGGCCT CCAGACCCCT GGGCAGACAG GACCGAGATG
    GGGAGGGTGG GGCATGAAGC CACCACAGAG CCTTGTCCGG ATCCCTCCTG GTATCTGACC
    ACAAGTGTCA CGATGACGAC AGACACCATG ACCAGTCCGG AGCACTGCCA GAAGCAGCCA
    CTGCGGACCC ATGTCCTCAC TGAGCAGTCT GGTGTGCACG TGCTGCACAG CCCGCCTGCC
    ATTCGCCGGG TTGACAGTAT AGCATCAGGC GGCGAGGGCA GGAGCCGAGC GGACCCCGAG
    GGCCCCTTTC CTATGAGCAG AGCAAGAGGG AACCTGGCAG ATGCCAAGGG GGGTCATCCA
    GAACCTGTGC TCAACAGGTG GCCTTGCACG CCACCCAGAC ATCCCCGGGA CTGTGTGGAG
    GGGTCTCTTC GCCCCCTCGA CAGACCTATC TGCCCCTCCC TGGGATTTGC CTCCCCTCTG
    AACAGCCTAG ATATGTCTAA GAACATGAGT GATGACATGA AGGAGGTGGC TTTTTCTGTC
    AGGAATGCCA TCTGCGCCGG TCCTGCCGAG CCACACGTCA GGGATATGGC CTGCCAGACC
    AATGGGTCCA GGACAGCAGG GACGCAGACC ATCCAGACAA TCAGCGTGGG CCTGCAGACC
    GAGGCCCTAC GTGCCAGCGG CGTCACCAGC AGCCCCCACA AGTGTCTCAC GCCAAAAGCC
    GGAGGTGGCA CCACACCCGT GTCTTCTCCT TCCCGCAGCC TTAGGAGTAG ACAGGTGGCC
    CCTGCCATTG AGAAGGTGCA GGCCAAGTTT GAACGCACCT GCTGCTCCCC AAAGTACGGT
    TCTCCCAAAC TTCAGAGAAA GCCCCTCTCA AAAGCTGACC AGCCAAATAG CAGGACCTCA
    CCAGGCATAC CCCAGAAAGG GTTCAGCGAG TCAGCCTGGG CCCGCTCCAC CACCACGAGG
    GAGAGCCCTG TGCACACCAC CATCAATGAT GGCCTCTCCA GCCTCTTCAA TATCATCGAC
    CACAGTCCCA TCGGGGTGCG GGCTGGGAGC CGATCTCGGT CAGCAGAACC TCGACAGGAG
    CTGGGTCCAG GCCAGGAAAC GGGCACCAGT TCCAGAGGGA GGTCGCCCAG CCCGCTAGGG
    GTAGGCTCCG AGACGTTCAG GGAGGAGGGA GGAGAGAGCA CGCCAGTGAG ACAGGACTTA
    TCTGCGCCCC CTGGCTACAC GCTCACTGAG AATGTGGCCC GGATCCTCAA CAAAAAGTTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAAG CAAGCCAGCC ATGGCTCCTC AGGTCTCACC
    AGCGACAGCC ACACTGGAGA ACCAGTGCCA GCCGAGCCAG GATCCATGGA GGAACTGCCT
    TGTTCTGCGC TCGCGCCCTC CCTAGAGCCC TGCTTTTCCA GGCCAGAGAG ACCAGCAAAC
    CGCCGCCTTC CATCCCGTTG GGCCCCACCT TCCCCCACTG CCTCACAGTC ACAGTCACCC
    GGACACCCGA TGTCCATGGA GGAACACGGT GAGGAGGACC CACCAGAGGA GAAGCCACAC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu70286
    Clone ID Related Accession (Same CDS sequence) XM_011246587.2 , XM_011246587.3
    Accession Version XM_011246587.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    CTCAGGGGCT CCCCAGTCTT ACCTGAGCAG AGTGTGTCTG TAGAAGAGCT ACAGGGTCAG
    CTCGAGCAGG CGGCCAGACT CCATCAAGAG GAGACAGAGA CATACACAAA CAAGATCCGT
    AAGATGGAGG AGGACCACCT CTACGCCCTG CGGTGGAAGG AACTGGAGAT GCACAGCCTG
    GCCCTGCAGA ACACCCTTCA CAAGCGGACG TGGAGCGACG AGAAGAATCT GCTGCAGCAG
    GAGCTCAGGT CCCTGAAGCA GAACATCTTC CTCTTCTACG TCAAGCTCAG GTGGCTGCTG
    AAACACTGGC GCCAAGGGAA GCAGATGGAG GAGGGAGGAG AGGATCTGGA GGAGAGCGAA
    CATCCAGAGA ATGTGCCTGG GCTTGCTGAG CTTGGAGTCC AGGGGGTTCA CCAGACAGAT
    GGGATAGACC AGGAGGATGC TGACCAAGGC TGTAGCCTTC CCATGGGAGA GCATGCCCCA
    CACTCCCTGG TACAGATCAG CGAACACGGA TCACGGCTAC AGAGTTCAGA CGGGGGACCA
    CTTAACAAAC AGGTGGTGGA AAACCAGCAG CTCTTCAGGG CCCTCAAGGC TCTGCTGGAG
    GACTTCCGTT CGGAGCTGCG CGAAGACGAG CATGCGCGGC TGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AGGCTGCGTG GGATGTGGAG TGGGCTGTGC TCAAGTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA AAGCTTGGGC GAGCTGGACT CCTCTGCTGA GGGCAAAGGG
    GCCTTGAAGA AGGAGAGAGA GGTGCACCAG AAACTGCTGG CTGACAGCCA CAGCCTGGTG
    ATGGACCTGC GCTGGCAGAT CCATCACCGA GAGAAGAACT GGAACCGGGA GAAGGTGGAG
    CTGCTGGAGC GTCTGGACAG CGAGCGGCAG GAGTGGGGGC GACAGAAGGA GGAGCTCCTG
    TGGCGGGTGG AGCAGTTACA GAAAGAGAAA AGTCCCCGGA GAAGCGGCAG TTTCCTCTGC
    AGCCGAAGGG AAGACGACAC ACGCCCTTAC CCGCACCAAG GAAGCCTTCA CTCCTCCAGA
    CCTGTGTCCA TGTGGCCCTG TGAAGACGCC GACTCCATCC CATTTGAAGA CCGGCCGCTC
    TCCAAACTGA AGGAGTCGGA CAGGTGCTCG GCCAGTGAGA ACCTGTACTT GGACGCTTTG
    TCCCTGGATG ATGATCCAGG GGATCCGCCA CCCCTCAGGA ACTGCCTTGC CGAGGAAGAA
    GAAAGTCGCA AGGGCAATCT TCAGAGGGCT GTGTCTGTGT CCTGTATGTC CGAGTTCCAG
    CGTCTGATGG ACGTCTCTCC GTTCCTGCCT GAGAAGGGGC TACCTTCCGC CGGCAGCAAG
    GAGGATGTCA CTCCACCCTT GTCTCCCGAC GACTTGAAAT ACATTGAAGA ATTCCATAGC
    AACGACTGGG ATTACGCATC CCCCAGGGCT GAGGCCGATC GGCCTCCAGA CCCCTGGGCA
    GACAGGACCG AGATGGGGAG GGTGGGGCAT GAAGCCACCA CAGAGCCTTG TCCGGATCCC
    TCCTGGTATC TGACCACAAG TGTCACGATG ACGACAGACA CCATGACCAG TCCGGAGCAC
    TGCCAGAAGC AGCCACTGCG GACCCATGTC CTCACTGAGC AGTCTGGTGT GCACGTGCTG
    CACAGCCCGC CTGCCATTCG CCGGGTTGAC AGTATAGCAT CAGGCGGCGA GGGCAGGAGC
    CGAGCGGACC CCGAGGGCCC CTTTCCTATG AGCAGAGCAA GAGGGAACCT GGCAGATGCC
    AAGGGGGGTC ATCCAGAACC TGTGCTCAAC AGGTGGCCTT GCACGCCACC CAGACATCCC
    CGGGACTGTG TGGAGGGGTC TCTTCGCCCC CTCGACAGAC CTATCTGCCC CTCCCTGGGA
    TTTGCCTCCC CTCTGAACAG CCTAGATATG TCTAAGAACA TGAGTGATGA CATGAAGGAG
    GTGGCTTTTT CTGTCAGGAA TGCCATCTGC GCCGGTCCTG CCGAGCCACA CGTCAGGGAT
    ATGGCCTGCC AGACCAATGG GTCCAGGACA GCAGGGACGC AGACCATCCA GACAATCAGC
    GTGGGCCTGC AGACCGAGGC CCTACGTGCC AGCGGCGTCA CCAGCAGCCC CCACAAGTGT
    CTCACGCCAA AAGCCGGAGG TGGCACCACA CCCGTGTCTT CTCCTTCCCG CAGCCTTAGG
    AGTAGACAGG TGGCCCCTGC CATTGAGAAG GTGCAGGCCA AGTTTGAACG CACCTGCTGC
    TCCCCAAAGT ACGGTTCTCC CAAACTTCAG AGAAAGCCCC TCTCAAAAGC TGACCAGCCA
    AATAGCAGGA CCTCACCAGG CATACCCCAG AAAGGGTTCA GCGAGTCAGC CTGGGCCCGC
    TCCACCACCA CGAGGGAGAG CCCTGTGCAC ACCACCATCA ATGATGGCCT CTCCAGCCTC
    TTCAATATCA TCGACCACAG TCCCATCGGG GTGCGGGCTG GGAGCCGATC TCGGTCAGCA
    GAACCTCGAC AGGAGCTGGG TCCAGGCCAG GAAACGGGCA CCAGTTCCAG AGGGAGGTCG
    CCCAGCCCGC TAGGGGTAGG CTCCGAGACG TTCAGGGAGG AGGGAGGAGA GAGCACGCCA
    GTGAGACAGG ACTTATCTGC GCCCCCTGGC TACACGCTCA CTGAGAATGT GGCCCGGATC
    CTCAACAAAA AGTTGTTGGA GCATGCCTTA AAGGAGGAGA GGAAGCAAGC CAGCCATGGC
    TCCTCAGGTC TCACCAGCGA CAGCCACACT GGAGAACCAG TGCCAGCCGA GCCAGGATCC
    ATGGAGAACC AAACTGTCTT ACTAACTGCC CCCTGGGGAC TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244889.1
    CDS461..6265
    Translation

    Target ORF information:

    RefSeq Version XM_011246587.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246587.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    CTCAGGGGCT CCCCAGTCTT ACCTGAGCAG AGTGTGTCTG TAGAAGAGCT ACAGGGTCAG
    CTCGAGCAGG CGGCCAGACT CCATCAAGAG GAGACAGAGA CATACACAAA CAAGATCCGT
    AAGATGGAGG AGGACCACCT CTACGCCCTG CGGTGGAAGG AACTGGAGAT GCACAGCCTG
    GCCCTGCAGA ACACCCTTCA CAAGCGGACG TGGAGCGACG AGAAGAATCT GCTGCAGCAG
    GAGCTCAGGT CCCTGAAGCA GAACATCTTC CTCTTCTACG TCAAGCTCAG GTGGCTGCTG
    AAACACTGGC GCCAAGGGAA GCAGATGGAG GAGGGAGGAG AGGATCTGGA GGAGAGCGAA
    CATCCAGAGA ATGTGCCTGG GCTTGCTGAG CTTGGAGTCC AGGGGGTTCA CCAGACAGAT
    GGGATAGACC AGGAGGATGC TGACCAAGGC TGTAGCCTTC CCATGGGAGA GCATGCCCCA
    CACTCCCTGG TACAGATCAG CGAACACGGA TCACGGCTAC AGAGTTCAGA CGGGGGACCA
    CTTAACAAAC AGGTGGTGGA AAACCAGCAG CTCTTCAGGG CCCTCAAGGC TCTGCTGGAG
    GACTTCCGTT CGGAGCTGCG CGAAGACGAG CATGCGCGGC TGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AGGCTGCGTG GGATGTGGAG TGGGCTGTGC TCAAGTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA AAGCTTGGGC GAGCTGGACT CCTCTGCTGA GGGCAAAGGG
    GCCTTGAAGA AGGAGAGAGA GGTGCACCAG AAACTGCTGG CTGACAGCCA CAGCCTGGTG
    ATGGACCTGC GCTGGCAGAT CCATCACCGA GAGAAGAACT GGAACCGGGA GAAGGTGGAG
    CTGCTGGAGC GTCTGGACAG CGAGCGGCAG GAGTGGGGGC GACAGAAGGA GGAGCTCCTG
    TGGCGGGTGG AGCAGTTACA GAAAGAGAAA AGTCCCCGGA GAAGCGGCAG TTTCCTCTGC
    AGCCGAAGGG AAGACGACAC ACGCCCTTAC CCGCACCAAG GAAGCCTTCA CTCCTCCAGA
    CCTGTGTCCA TGTGGCCCTG TGAAGACGCC GACTCCATCC CATTTGAAGA CCGGCCGCTC
    TCCAAACTGA AGGAGTCGGA CAGGTGCTCG GCCAGTGAGA ACCTGTACTT GGACGCTTTG
    TCCCTGGATG ATGATCCAGG GGATCCGCCA CCCCTCAGGA ACTGCCTTGC CGAGGAAGAA
    GAAAGTCGCA AGGGCAATCT TCAGAGGGCT GTGTCTGTGT CCTGTATGTC CGAGTTCCAG
    CGTCTGATGG ACGTCTCTCC GTTCCTGCCT GAGAAGGGGC TACCTTCCGC CGGCAGCAAG
    GAGGATGTCA CTCCACCCTT GTCTCCCGAC GACTTGAAAT ACATTGAAGA ATTCCATAGC
    AACGACTGGG ATTACGCATC CCCCAGGGCT GAGGCCGATC GGCCTCCAGA CCCCTGGGCA
    GACAGGACCG AGATGGGGAG GGTGGGGCAT GAAGCCACCA CAGAGCCTTG TCCGGATCCC
    TCCTGGTATC TGACCACAAG TGTCACGATG ACGACAGACA CCATGACCAG TCCGGAGCAC
    TGCCAGAAGC AGCCACTGCG GACCCATGTC CTCACTGAGC AGTCTGGTGT GCACGTGCTG
    CACAGCCCGC CTGCCATTCG CCGGGTTGAC AGTATAGCAT CAGGCGGCGA GGGCAGGAGC
    CGAGCGGACC CCGAGGGCCC CTTTCCTATG AGCAGAGCAA GAGGGAACCT GGCAGATGCC
    AAGGGGGGTC ATCCAGAACC TGTGCTCAAC AGGTGGCCTT GCACGCCACC CAGACATCCC
    CGGGACTGTG TGGAGGGGTC TCTTCGCCCC CTCGACAGAC CTATCTGCCC CTCCCTGGGA
    TTTGCCTCCC CTCTGAACAG CCTAGATATG TCTAAGAACA TGAGTGATGA CATGAAGGAG
    GTGGCTTTTT CTGTCAGGAA TGCCATCTGC GCCGGTCCTG CCGAGCCACA CGTCAGGGAT
    ATGGCCTGCC AGACCAATGG GTCCAGGACA GCAGGGACGC AGACCATCCA GACAATCAGC
    GTGGGCCTGC AGACCGAGGC CCTACGTGCC AGCGGCGTCA CCAGCAGCCC CCACAAGTGT
    CTCACGCCAA AAGCCGGAGG TGGCACCACA CCCGTGTCTT CTCCTTCCCG CAGCCTTAGG
    AGTAGACAGG TGGCCCCTGC CATTGAGAAG GTGCAGGCCA AGTTTGAACG CACCTGCTGC
    TCCCCAAAGT ACGGTTCTCC CAAACTTCAG AGAAAGCCCC TCTCAAAAGC TGACCAGCCA
    AATAGCAGGA CCTCACCAGG CATACCCCAG AAAGGGTTCA GCGAGTCAGC CTGGGCCCGC
    TCCACCACCA CGAGGGAGAG CCCTGTGCAC ACCACCATCA ATGATGGCCT CTCCAGCCTC
    TTCAATATCA TCGACCACAG TCCCATCGGG GTGCGGGCTG GGAGCCGATC TCGGTCAGCA
    GAACCTCGAC AGGAGCTGGG TCCAGGCCAG GAAACGGGCA CCAGTTCCAG AGGGAGGTCG
    CCCAGCCCGC TAGGGGTAGG CTCCGAGACG TTCAGGGAGG AGGGAGGAGA GAGCACGCCA
    GTGAGACAGG ACTTATCTGC GCCCCCTGGC TACACGCTCA CTGAGAATGT GGCCCGGATC
    CTCAACAAAA AGTTGTTGGA GCATGCCTTA AAGGAGGAGA GGAAGCAAGC CAGCCATGGC
    TCCTCAGGTC TCACCAGCGA CAGCCACACT GGAGAACCAG TGCCAGCCGA GCCAGGATCC
    ATGGAGAACC AAACTGTCTT ACTAACTGCC CCCTGGGGAC TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79656
    Clone ID Related Accession (Same CDS sequence) XM_017317622.1 , XM_017317622.2
    Accession Version XM_017317622.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGAT GGAGGAGGAC
    CACCTCTACG CCCTGCGGTG GAAGGAACTG GAGATGCACA GCCTGGCCCT GCAGAACACC
    CTTCACAAGC GGACGTGGAG CGACGAGAAG AATCTGCTGC AGCAGGAGCT CAGGTCCCTG
    AAGCAGAACA TCTTCCTCTT CTACGTCAAG CTCAGGTGGC TGCTGAAACA CTGGCGCCAA
    GGGAAGCAGA TGGAGGAGGG AGGAGAGGAT CTGGAGGAGA GCGAACATCC AGAGAATGTG
    CCTGGGCTTG CTGAGCTTGG AGTCCAGGGG GTTCACCAGA CAGATGGGAT AGACCAGGAG
    GATGCTGACC AAGGCTGTAG CCTTCCCATG GGAGAGCATG CCCCACACTC CCTGGTACAG
    ATCAGCGAAC ACGGATCACG GCTACAGAGT TCAGACGGGG GACCACTTAA CAAACAGGTG
    GTGGAAAACC AGCAGCTCTT CAGGGCCCTC AAGGCTCTGC TGGAGGACTT CCGTTCGGAG
    CTGCGCGAAG ACGAGCATGC GCGGCTGCGG CTGCAGCAGC AGTACGCCAG CGACAAGGCT
    GCGTGGGATG TGGAGTGGGC TGTGCTCAAG TGCCGCCTGG AACAGCTGGA AGAGAAGACT
    GAGAAAAGCT TGGGCGAGCT GGACTCCTCT GCTGAGGGCA AAGGGGCCTT GAAGAAGGAG
    AGAGAGGTGC ACCAGAAACT GCTGGCTGAC AGCCACAGCC TGGTGATGGA CCTGCGCTGG
    CAGATCCATC ACCGAGAGAA GAACTGGAAC CGGGAGAAGG TGGAGCTGCT GGAGCGTCTG
    GACAGCGAGC GGCAGGAGTG GGGGCGACAG AAGGAGGAGC TCCTGTGGCG GGTGGAGCAG
    TTACAGAAAG AGAAAAGTCC CCGGAGAAGC GGCAGTTTCC TCTGCAGCCG AAGGGAAGAC
    GACACACGCC CTTACCCGCA CCAAGGAAGC CTTCACTCCT CCAGACCTGT GTCCATGTGG
    CCCTGTGAAG ACGCCGACTC CATCCCATTT GAAGACCGGC CGCTCTCCAA ACTGAAGGAG
    TCGGACAGGT GCTCGGCCAG TGAGAACCTG TACTTGGACG CTTTGTCCCT GGATGATGAT
    CCAGGGGATC CGCCACCCCT CAGGAACTGC CTTGCCGAGG AAGAAGAAAG TCGCAAGGGC
    AATCTTCAGA GGGCTGTGTC TGTGTCCTGT ATGTCCGAGT TCCAGCGTCT GATGGACGTC
    TCTCCGTTCC TGCCTGAGAA GGGGCTACCT TCCGCCGGCA GCAAGGAGGA TGTCACTCCA
    CCCTTGTCTC CCGACGACTT GAAATACATT GAAGAATTCC ATAGCAACGA CTGGGATTAC
    GCATCCCCCA GGGCTGAGGC CGATCGGCCT CCAGACCCCT GGGCAGACAG GACCGAGATG
    GGGAGGGTGG GGCATGAAGC CACCACAGAG CCTTGTCCGG ATCCCTCCTG GTATCTGACC
    ACAAGTGTCA CGATGACGAC AGACACCATG ACCAGTCCGG AGCACTGCCA GAAGCAGCCA
    CTGCGGACCC ATGTCCTCAC TGAGCAGTCT GGTGTGCACG TGCTGCACAG CCCGCCTGCC
    ATTCGCCGGG TTGACAGTAT AGCATCAGGC GGCGAGGGCA GGAGCCGAGC GGACCCCGAG
    GGCCCCTTTC CTATGAGCAG AGCAAGAGGG AACCTGGCAG ATGCCAAGGG GGGTCATCCA
    GAACCTGTGC TCAACAGGTG GCCTTGCACG CCACCCAGAC ATCCCCGGGA CTGTGTGGAG
    GGGTCTCTTC GCCCCCTCGA CAGACCTATC TGCCCCTCCC TGGGATTTGC CTCCCCTCTG
    AACAGCCTAG ATATGTCTAA GAACATGAGT GATGACATGA AGGAGGTGGC TTTTTCTGTC
    AGGAATGCCA TCTGCGCCGG TCCTGCCGAG CCACACGTCA GGGATATGGC CTGCCAGACC
    AATGGGTCCA GGACAGCAGG GACGCAGACC ATCCAGACAA TCAGCGTGGG CCTGCAGACC
    GAGGCCCTAC GTGCCAGCGG CGTCACCAGC AGCCCCCACA AGTGTCTCAC GCCAAAAGCC
    GGAGGTGGCA CCACACCCGT GTCTTCTCCT TCCCGCAGCC TTAGGAGTAG ACAGGTGGCC
    CCTGCCATTG AGAAGGTGCA GGCCAAGTTT GAACGCACCT GCTGCTCCCC AAAGTACGGT
    TCTCCCAAAC TTCAGAGAAA GCCCCTCTCA AAAGCTGACC AGCCAAATAG CAGGACCTCA
    CCAGGCATAC CCCAGAAAGG GTTCAGCGAG TCAGCCTGGG CCCGCTCCAC CACCACGAGG
    GAGAGCCCTG TGCACACCAC CATCAATGAT GGCCTCTCCA GCCTCTTCAA TATCATCGAC
    CACAGTCCCA TCGGGGTGCG GGCTGGGAGC CGATCTCGGT CAGCAGAACC TCGACAGGAG
    CTGGGTCCAG GCCAGGAAAC GGGCACCAGT TCCAGAGGGA GGTCGCCCAG CCCGCTAGGG
    GTAGGCTCCG AGACGTTCAG GGAGGAGGGA GGAGAGAGCA CGCCAGTGAG ACAGGACTTA
    TCTGCGCCCC CTGGCTACAC GCTCACTGAG AATGTGGCCC GGATCCTCAA CAAAAAGTTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAAG CAAGCCAGCC ATGGCTCCTC AGGTCTCACC
    AGCGACAGCC ACACTGGAGA ACCAGTGCCA GCCGAGCCAG GATCCATGGA GAACCAAACT
    GTCTTACTAA CTGCCCCCTG GGGACTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173111.1
    CDS461..6250
    Translation

    Target ORF information:

    RefSeq Version XM_017317622.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317622.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGAT GGAGGAGGAC
    CACCTCTACG CCCTGCGGTG GAAGGAACTG GAGATGCACA GCCTGGCCCT GCAGAACACC
    CTTCACAAGC GGACGTGGAG CGACGAGAAG AATCTGCTGC AGCAGGAGCT CAGGTCCCTG
    AAGCAGAACA TCTTCCTCTT CTACGTCAAG CTCAGGTGGC TGCTGAAACA CTGGCGCCAA
    GGGAAGCAGA TGGAGGAGGG AGGAGAGGAT CTGGAGGAGA GCGAACATCC AGAGAATGTG
    CCTGGGCTTG CTGAGCTTGG AGTCCAGGGG GTTCACCAGA CAGATGGGAT AGACCAGGAG
    GATGCTGACC AAGGCTGTAG CCTTCCCATG GGAGAGCATG CCCCACACTC CCTGGTACAG
    ATCAGCGAAC ACGGATCACG GCTACAGAGT TCAGACGGGG GACCACTTAA CAAACAGGTG
    GTGGAAAACC AGCAGCTCTT CAGGGCCCTC AAGGCTCTGC TGGAGGACTT CCGTTCGGAG
    CTGCGCGAAG ACGAGCATGC GCGGCTGCGG CTGCAGCAGC AGTACGCCAG CGACAAGGCT
    GCGTGGGATG TGGAGTGGGC TGTGCTCAAG TGCCGCCTGG AACAGCTGGA AGAGAAGACT
    GAGAAAAGCT TGGGCGAGCT GGACTCCTCT GCTGAGGGCA AAGGGGCCTT GAAGAAGGAG
    AGAGAGGTGC ACCAGAAACT GCTGGCTGAC AGCCACAGCC TGGTGATGGA CCTGCGCTGG
    CAGATCCATC ACCGAGAGAA GAACTGGAAC CGGGAGAAGG TGGAGCTGCT GGAGCGTCTG
    GACAGCGAGC GGCAGGAGTG GGGGCGACAG AAGGAGGAGC TCCTGTGGCG GGTGGAGCAG
    TTACAGAAAG AGAAAAGTCC CCGGAGAAGC GGCAGTTTCC TCTGCAGCCG AAGGGAAGAC
    GACACACGCC CTTACCCGCA CCAAGGAAGC CTTCACTCCT CCAGACCTGT GTCCATGTGG
    CCCTGTGAAG ACGCCGACTC CATCCCATTT GAAGACCGGC CGCTCTCCAA ACTGAAGGAG
    TCGGACAGGT GCTCGGCCAG TGAGAACCTG TACTTGGACG CTTTGTCCCT GGATGATGAT
    CCAGGGGATC CGCCACCCCT CAGGAACTGC CTTGCCGAGG AAGAAGAAAG TCGCAAGGGC
    AATCTTCAGA GGGCTGTGTC TGTGTCCTGT ATGTCCGAGT TCCAGCGTCT GATGGACGTC
    TCTCCGTTCC TGCCTGAGAA GGGGCTACCT TCCGCCGGCA GCAAGGAGGA TGTCACTCCA
    CCCTTGTCTC CCGACGACTT GAAATACATT GAAGAATTCC ATAGCAACGA CTGGGATTAC
    GCATCCCCCA GGGCTGAGGC CGATCGGCCT CCAGACCCCT GGGCAGACAG GACCGAGATG
    GGGAGGGTGG GGCATGAAGC CACCACAGAG CCTTGTCCGG ATCCCTCCTG GTATCTGACC
    ACAAGTGTCA CGATGACGAC AGACACCATG ACCAGTCCGG AGCACTGCCA GAAGCAGCCA
    CTGCGGACCC ATGTCCTCAC TGAGCAGTCT GGTGTGCACG TGCTGCACAG CCCGCCTGCC
    ATTCGCCGGG TTGACAGTAT AGCATCAGGC GGCGAGGGCA GGAGCCGAGC GGACCCCGAG
    GGCCCCTTTC CTATGAGCAG AGCAAGAGGG AACCTGGCAG ATGCCAAGGG GGGTCATCCA
    GAACCTGTGC TCAACAGGTG GCCTTGCACG CCACCCAGAC ATCCCCGGGA CTGTGTGGAG
    GGGTCTCTTC GCCCCCTCGA CAGACCTATC TGCCCCTCCC TGGGATTTGC CTCCCCTCTG
    AACAGCCTAG ATATGTCTAA GAACATGAGT GATGACATGA AGGAGGTGGC TTTTTCTGTC
    AGGAATGCCA TCTGCGCCGG TCCTGCCGAG CCACACGTCA GGGATATGGC CTGCCAGACC
    AATGGGTCCA GGACAGCAGG GACGCAGACC ATCCAGACAA TCAGCGTGGG CCTGCAGACC
    GAGGCCCTAC GTGCCAGCGG CGTCACCAGC AGCCCCCACA AGTGTCTCAC GCCAAAAGCC
    GGAGGTGGCA CCACACCCGT GTCTTCTCCT TCCCGCAGCC TTAGGAGTAG ACAGGTGGCC
    CCTGCCATTG AGAAGGTGCA GGCCAAGTTT GAACGCACCT GCTGCTCCCC AAAGTACGGT
    TCTCCCAAAC TTCAGAGAAA GCCCCTCTCA AAAGCTGACC AGCCAAATAG CAGGACCTCA
    CCAGGCATAC CCCAGAAAGG GTTCAGCGAG TCAGCCTGGG CCCGCTCCAC CACCACGAGG
    GAGAGCCCTG TGCACACCAC CATCAATGAT GGCCTCTCCA GCCTCTTCAA TATCATCGAC
    CACAGTCCCA TCGGGGTGCG GGCTGGGAGC CGATCTCGGT CAGCAGAACC TCGACAGGAG
    CTGGGTCCAG GCCAGGAAAC GGGCACCAGT TCCAGAGGGA GGTCGCCCAG CCCGCTAGGG
    GTAGGCTCCG AGACGTTCAG GGAGGAGGGA GGAGAGAGCA CGCCAGTGAG ACAGGACTTA
    TCTGCGCCCC CTGGCTACAC GCTCACTGAG AATGTGGCCC GGATCCTCAA CAAAAAGTTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAAG CAAGCCAGCC ATGGCTCCTC AGGTCTCACC
    AGCGACAGCC ACACTGGAGA ACCAGTGCCA GCCGAGCCAG GATCCATGGA GAACCAAACT
    GTCTTACTAA CTGCCCCCTG GGGACTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53971
    Clone ID Related Accession (Same CDS sequence) XM_006524849.3 , XM_006524849.2
    Accession Version XM_006524849.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGCT CAGGGGCTCC
    CCAGTCTTAC CTGAGCAGAG TGTGTCTGTA GAAGAGCTAC AGGGTCAGCT CGAGCAGGCG
    GCCAGACTCC ATCAAGAGGA GACAGAGACA TACACAAACA AGATCCGTAA GATGGAGGAG
    GACCACCTCT ACGCCCTGCG GTGGAAGGAA CTGGAGATGC ACAGCCTGGC CCTGCAGAAC
    ACCCTTCACA AGCGGACGTG GAGCGACGAG AAGAATCTGC TGCAGCAGGA GCTCAGGTCC
    CTGAAGCAGA ACATCTTCCT CTTCTACGTC AAGCTCAGGT GGCTGCTGAA ACACTGGCGC
    CAAGGGAAGC AGATGGAGGA GGGAGGAGAG GATCTGGAGG AGAGCGAACA TCCAGAGAAT
    GTGCCTGGGC TTGCTGAGCT TGGAGTCCAG GGGGTTCACC AGACAGATGG GATAGACCAG
    GAGGATGCTG ACCAAGGCTG TAGCCTTCCC ATGGGAGAGC ATGCCCCACA CTCCCTGGTA
    CAGATCAGCG AACACGGATC ACGGCTACAG AGTTCAGACG GGGGACCACT TAACAAACAG
    GTGGTGGAAA ACCAGCAGCT CTTCAGGGCC CTCAAGGCTC TGCTGGAGGA CTTCCGTTCG
    GAGCTGCGCG AAGACGAGCA TGCGCGGCTG CGGCTGCAGC AGCAGTACGC CAGCGACAAG
    GCTGCGTGGG ATGTGGAGTG GGCTGTGCTC AAGTGCCGCC TGGAACAGCT GGAAGAGAAG
    ACTGAGAAAA GCTTGGGCGA GCTGGACTCC TCTGCTGAGG GCAAAGGGGC CTTGAAGAAG
    GAGAGAGAGG TGCACCAGAA ACTGCTGGCT GACAGCCACA GCCTGGTGAT GGACCTGCGC
    TGGCAGATCC ATCACCGAGA GAAGAACTGG AACCGGGAGA AGGTGGAGCT GCTGGAGCGT
    CTGGACAGCG AGCGGCAGGA GTGGGGGCGA CAGAAGGAGG AGCTCCTGTG GCGGGTGGAG
    CAGTTACAGA AAGAGAAAAG TCCCCGGAGA AGCGGCAGTT TCCTCTGCAG CCGAAGGGAA
    GACGACACAC GCCCTTACCC GCACCAAGGA AGCCTTCACT CCTCCAGACC TGTGTCCATG
    TGGCCCTGTG AAGACGCCGA CTCCATCCCA TTTGAAGACC GGCCGCTCTC CAAACTGAAG
    GAGTCGGACA GGTGCTCGGC CAGTGAGAAC CTGTACTTGG ACGCTTTGTC CCTGGATGAT
    GATCCAGGGG ATCCGCCACC CCTCAGGAAC TGCCTTGCCG AGGAAGAAGA AAGTCGCAAG
    GGCAATCTTC AGAGGGCTGT GTCTGTGTCC TGTATGTCCG AGTTCCAGCG TCTGATGGAC
    GTCTCTCCGT TCCTGCCTGA GAAGGGGCTA CCTTCCGCCG GCAGCAAGGA GGATGTCACT
    CCACCCTTGT CTCCCGACGA CTTGAAATAC ATTGAAGAAT TCCATAGCAA CGACTGGGAT
    TACGCATCCC CCAGGGCTGA GGCCGATCGG CCTCCAGACC CCTGGGCAGA CAGGACCGAG
    ATGGGGAGGG TGGGGCATGA AGCCACCACA GAGCCTTGTC CGGATCCCTC CTGGTATCTG
    ACCACAAGTG TCACGATGAC GACAGACACC ATGACCAGTC CGGAGCACTG CCAGAAGCAG
    CCACTGCGGA CCCATGTCCT CACTGAGCAG TCTGGTGTGC ACGTGCTGCA CAGCCCGCCT
    GCCATTCGCC GGGTTGACAG TATAGCATCA GGCGGCGAGG GCAGGAGCCG AGCGGACCCC
    GAGGGCCCCT TTCCTATGAG CAGAGCAAGA GGGAACCTGG CAGATGCCAA GGGGGGTCAT
    CCAGAACCTG TGCTCAACAG GTGGCCTTGC ACGCCACCCA GACATCCCCG GGACTGTGTG
    GAGGGGTCTC TTCGCCCCCT CGACAGACCT ATCTGCCCCT CCCTGGGATT TGCCTCCCCT
    CTGAACAGCC TAGATATGTC TAAGAACATG AGTGATGACA TGAAGGAGGT GGCTTTTTCT
    GTCAGGAATG CCATCTGCGC CGGTCCTGCC GAGCCACACG TCAGGGATAT GGCCTGCCAG
    ACCAATGGGT CCAGGACAGC AGGGACGCAG ACCATCCAGA CAATCAGCGT GGGCCTGCAG
    ACCGAGGCCC TACGTGCCAG CGGCGTCACC AGCAGCCCCC ACAAGTGTCT CACGCCAAAA
    GCCGGAGGTG GCACCACACC CGTGTCTTCT CCTTCCCGCA GCCTTAGGAG TAGACAGGTG
    GCCCCTGCCA TTGAGAAGGT GCAGGCCAAG TTTGAACGCA CCTGCTGCTC CCCAAAGTAC
    GGTTCTCCCA AACTTCAGAG AAAGCCCCTC TCAAAAGCTG ACCAGCCAAA TAGCAGGACC
    TCACCAGGCA TACCCCAGAA AGGGTTCAGC GAGTCAGCCT GGGCCCGCTC CACCACCACG
    AGGGAGAGCC CTGTGCACAC CACCATCAAT GATGGCCTCT CCAGCCTCTT CAATATCATC
    GACCACAGTC CCATCGGGGT GCGGGCTGGG AGCCGATCTC GGTCAGCAGA ACCTCGACAG
    GAGCTGGGTC CAGGCCAGGA AACGGGCACC AGTTCCAGAG GGAGGTCGCC CAGCCCGCTA
    GGGGTAGGCT CCGAGACGTT CAGGGAGGAG GGAGGAGAGA GCACGCCAGT GAGACAGGAC
    TTATCTGCGC CCCCTGGCTA CACGCTCACT GAGAATGTGG CCCGGATCCT CAACAAAAAG
    TTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AAGCAAGCCA GCCATGGCTC CTCAGGTCTC
    ACCAGCGACA GCCACACTGG AGAACCAGTG CCAGCCGAGC CAGGATCCAT GGAGGAACTG
    CCTTGTTCTG CGCTCGCGCC CTCCCTAGAG CCCTGCTTTT CCAGGCCAGA GAGACCAGCA
    AACCGCCGCC TTCCATCCCG TTGGGCCCCA CCTTCCCCCA CTGCCTCACA GTCACAGTCA
    CCCGGACACC CGATGTCCAT GGAGGAACAC GGTGAGGAGG ACCCACCAGA GGAGAAGCCA
    CACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524912.1
    CDS461..6529
    Translation

    Target ORF information:

    RefSeq Version XM_006524849.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524849.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGCT CAGGGGCTCC
    CCAGTCTTAC CTGAGCAGAG TGTGTCTGTA GAAGAGCTAC AGGGTCAGCT CGAGCAGGCG
    GCCAGACTCC ATCAAGAGGA GACAGAGACA TACACAAACA AGATCCGTAA GATGGAGGAG
    GACCACCTCT ACGCCCTGCG GTGGAAGGAA CTGGAGATGC ACAGCCTGGC CCTGCAGAAC
    ACCCTTCACA AGCGGACGTG GAGCGACGAG AAGAATCTGC TGCAGCAGGA GCTCAGGTCC
    CTGAAGCAGA ACATCTTCCT CTTCTACGTC AAGCTCAGGT GGCTGCTGAA ACACTGGCGC
    CAAGGGAAGC AGATGGAGGA GGGAGGAGAG GATCTGGAGG AGAGCGAACA TCCAGAGAAT
    GTGCCTGGGC TTGCTGAGCT TGGAGTCCAG GGGGTTCACC AGACAGATGG GATAGACCAG
    GAGGATGCTG ACCAAGGCTG TAGCCTTCCC ATGGGAGAGC ATGCCCCACA CTCCCTGGTA
    CAGATCAGCG AACACGGATC ACGGCTACAG AGTTCAGACG GGGGACCACT TAACAAACAG
    GTGGTGGAAA ACCAGCAGCT CTTCAGGGCC CTCAAGGCTC TGCTGGAGGA CTTCCGTTCG
    GAGCTGCGCG AAGACGAGCA TGCGCGGCTG CGGCTGCAGC AGCAGTACGC CAGCGACAAG
    GCTGCGTGGG ATGTGGAGTG GGCTGTGCTC AAGTGCCGCC TGGAACAGCT GGAAGAGAAG
    ACTGAGAAAA GCTTGGGCGA GCTGGACTCC TCTGCTGAGG GCAAAGGGGC CTTGAAGAAG
    GAGAGAGAGG TGCACCAGAA ACTGCTGGCT GACAGCCACA GCCTGGTGAT GGACCTGCGC
    TGGCAGATCC ATCACCGAGA GAAGAACTGG AACCGGGAGA AGGTGGAGCT GCTGGAGCGT
    CTGGACAGCG AGCGGCAGGA GTGGGGGCGA CAGAAGGAGG AGCTCCTGTG GCGGGTGGAG
    CAGTTACAGA AAGAGAAAAG TCCCCGGAGA AGCGGCAGTT TCCTCTGCAG CCGAAGGGAA
    GACGACACAC GCCCTTACCC GCACCAAGGA AGCCTTCACT CCTCCAGACC TGTGTCCATG
    TGGCCCTGTG AAGACGCCGA CTCCATCCCA TTTGAAGACC GGCCGCTCTC CAAACTGAAG
    GAGTCGGACA GGTGCTCGGC CAGTGAGAAC CTGTACTTGG ACGCTTTGTC CCTGGATGAT
    GATCCAGGGG ATCCGCCACC CCTCAGGAAC TGCCTTGCCG AGGAAGAAGA AAGTCGCAAG
    GGCAATCTTC AGAGGGCTGT GTCTGTGTCC TGTATGTCCG AGTTCCAGCG TCTGATGGAC
    GTCTCTCCGT TCCTGCCTGA GAAGGGGCTA CCTTCCGCCG GCAGCAAGGA GGATGTCACT
    CCACCCTTGT CTCCCGACGA CTTGAAATAC ATTGAAGAAT TCCATAGCAA CGACTGGGAT
    TACGCATCCC CCAGGGCTGA GGCCGATCGG CCTCCAGACC CCTGGGCAGA CAGGACCGAG
    ATGGGGAGGG TGGGGCATGA AGCCACCACA GAGCCTTGTC CGGATCCCTC CTGGTATCTG
    ACCACAAGTG TCACGATGAC GACAGACACC ATGACCAGTC CGGAGCACTG CCAGAAGCAG
    CCACTGCGGA CCCATGTCCT CACTGAGCAG TCTGGTGTGC ACGTGCTGCA CAGCCCGCCT
    GCCATTCGCC GGGTTGACAG TATAGCATCA GGCGGCGAGG GCAGGAGCCG AGCGGACCCC
    GAGGGCCCCT TTCCTATGAG CAGAGCAAGA GGGAACCTGG CAGATGCCAA GGGGGGTCAT
    CCAGAACCTG TGCTCAACAG GTGGCCTTGC ACGCCACCCA GACATCCCCG GGACTGTGTG
    GAGGGGTCTC TTCGCCCCCT CGACAGACCT ATCTGCCCCT CCCTGGGATT TGCCTCCCCT
    CTGAACAGCC TAGATATGTC TAAGAACATG AGTGATGACA TGAAGGAGGT GGCTTTTTCT
    GTCAGGAATG CCATCTGCGC CGGTCCTGCC GAGCCACACG TCAGGGATAT GGCCTGCCAG
    ACCAATGGGT CCAGGACAGC AGGGACGCAG ACCATCCAGA CAATCAGCGT GGGCCTGCAG
    ACCGAGGCCC TACGTGCCAG CGGCGTCACC AGCAGCCCCC ACAAGTGTCT CACGCCAAAA
    GCCGGAGGTG GCACCACACC CGTGTCTTCT CCTTCCCGCA GCCTTAGGAG TAGACAGGTG
    GCCCCTGCCA TTGAGAAGGT GCAGGCCAAG TTTGAACGCA CCTGCTGCTC CCCAAAGTAC
    GGTTCTCCCA AACTTCAGAG AAAGCCCCTC TCAAAAGCTG ACCAGCCAAA TAGCAGGACC
    TCACCAGGCA TACCCCAGAA AGGGTTCAGC GAGTCAGCCT GGGCCCGCTC CACCACCACG
    AGGGAGAGCC CTGTGCACAC CACCATCAAT GATGGCCTCT CCAGCCTCTT CAATATCATC
    GACCACAGTC CCATCGGGGT GCGGGCTGGG AGCCGATCTC GGTCAGCAGA ACCTCGACAG
    GAGCTGGGTC CAGGCCAGGA AACGGGCACC AGTTCCAGAG GGAGGTCGCC CAGCCCGCTA
    GGGGTAGGCT CCGAGACGTT CAGGGAGGAG GGAGGAGAGA GCACGCCAGT GAGACAGGAC
    TTATCTGCGC CCCCTGGCTA CACGCTCACT GAGAATGTGG CCCGGATCCT CAACAAAAAG
    TTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AAGCAAGCCA GCCATGGCTC CTCAGGTCTC
    ACCAGCGACA GCCACACTGG AGAACCAGTG CCAGCCGAGC CAGGATCCAT GGAGGAACTG
    CCTTGTTCTG CGCTCGCGCC CTCCCTAGAG CCCTGCTTTT CCAGGCCAGA GAGACCAGCA
    AACCGCCGCC TTCCATCCCG TTGGGCCCCA CCTTCCCCCA CTGCCTCACA GTCACAGTCA
    CCCGGACACC CGATGTCCAT GGAGGAACAC GGTGAGGAGG ACCCACCAGA GGAGAAGCCA
    CACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23353
    Clone ID Related Accession (Same CDS sequence) NM_172963.4
    Accession Version NM_172963.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5838bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147205.1 and BP760436.1. On Jan 30, 2008 this sequence version replaced NM_172963.3. Transcript Variant: This variant (2) lacks an exon in the 3' region, which results in a downstream translation termination codon, compared to variant 1. The resulting isoform (2) is longer and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK147205.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    ATGGAGGAGG ACCACCTCTA CGCCCTGCGG TGGAAGGAAC TGGAGATGCA CAGCCTGGCC
    CTGCAGAACA CCCTTCACAA GCGGACGTGG AGCGACGAGA AGAATCTGCT GCAGCAGGAG
    CTCAGGTCCC TGAAGCAGAA CATCTTCCTC TTCTACGTCA AGCTCAGGTG GCTGCTGAAA
    CACTGGCGCC AAGGGAAGCA GATGGAGGAG GGAGGAGAGG ATCTGGAGGA GAGCGAACAT
    CCAGAGAATG TGCCTGGGCT TGCTGAGCTT GGAGTCCAGG GGGTTCACCA GACAGATGGG
    ATAGACCAGG AGGATGCTGA CCAAGGCTGT AGCCTTCCCA TGGGAGAGCA TGCCCCACAC
    TCCCTGGTAC AGATCAGCGA ACACGGATCA CGGCTACAGA GTTCAGACGG GGGACCACTT
    AACAAACAGG TGGTGGAAAA CCAGCAGCTC TTCAGGGCCC TCAAGGCTCT GCTGGAGGAC
    TTCCGTTCGG AGCTGCGCGA AGACGAGCAT GCGCGGCTGC GGCTGCAGCA GCAGTACGCC
    AGCGACAAGG CTGCGTGGGA TGTGGAGTGG GCTGTGCTCA AGTGCCGCCT GGAACAGCTG
    GAAGAGAAGA CTGAGAAAAG CTTGGGCGAG CTGGACTCCT CTGCTGAGGG CAAAGGGGCC
    TTGAAGAAGG AGAGAGAGGT GCACCAGAAA CTGCTGGCTG ACAGCCACAG CCTGGTGATG
    GACCTGCGCT GGCAGATCCA TCACCGAGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTG
    CTGGAGCGTC TGGACAGCGA GCGGCAGGAG TGGGGGCGAC AGAAGGAGGA GCTCCTGTGG
    CGGGTGGAGC AGTTACAGAA AGAGAAAAGT CCCCGGAGAA GCGGCAGTTT CCTCTGCAGC
    CGAAGGGAAG ACGACACACG CCCTTACCCG CACCAAGGAA GCCTTCACTC CTCCAGACCT
    GTGTCCATGT GGCCCTGTGA AGACGCCGAC TCCATCCCAT TTGAAGACCG GCCGCTCTCC
    AAACTGAAGG AGTCGGACAG GTGCTCGGCC AGTGAGAACC TGTACTTGGA CGCTTTGTCC
    CTGGATGATG ATCCAGGGGA TCCGCCACCC CTCAGGAACT GCCTTGCCGA GGAAGAAGAA
    AGTCGCAAGG GCAATCTTCA GAGGGCTGTG TCTGTGTCCT GTATGTCCGA GTTCCAGCGT
    CTGATGGACG TCTCTCCGTT CCTGCCTGAG AAGGGGCTAC CTTCCGCCGG CAGCAAGGAG
    GATGTCACTC CACCCTTGTC TCCCGACGAC TTGAAATACA TTGAAGAATT CCATAGCAAC
    GACTGGGATT ACGCATCCCC CAGGGCTGAG GCCGATCGGC CTCCAGACCC CTGGGCAGAC
    AGGACCGAGA TGGGGAGGGT GGGGCATGAA GCCACCACAG AGCCTTGTCC GGATCCCTCC
    TGGTATCTGA CCACAAGTGT CACGATGACG ACAGACACCA TGACCAGTCC GGAGCACTGC
    CAGAAGCAGC CACTGCGGAC CCATGTCCTC ACTGAGCAGT CTGGTGTGCA CGTGCTGCAC
    AGCCCGCCTG CCATTCGCCG GGTTGACAGT ATAGCATCAG GCGGCGAGGG CAGGAGCCGA
    GCGGACCCCG AGGGCCCCTT TCCTATGAGC AGAGCAAGAG GGAACCTGGC AGATGCCAAG
    GGGGGTCATC CAGAACCTGT GCTCAACAGG TGGCCTTGCA CGCCACCCAG ACATCCCCGG
    GACTGTGTGG AGGGGTCTCT TCGCCCCCTC GACAGACCTA TCTGCCCCTC CCTGGGATTT
    GCCTCCCCTC TGAACAGCCT AGATATGTCT AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCTTTTTCTG TCAGGAATGC CATCTGCGCC GGTCCTGCCG AGCCACACGT CAGGGATATG
    GCCTGCCAGA CCAATGGGTC CAGGACAGCA GGGACGCAGA CCATCCAGAC AATCAGCGTG
    GGCCTGCAGA CCGAGGCCCT ACGTGCCAGC GGCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACGCCAAAAG CCGGAGGTGG CACCACACCC GTGTCTTCTC CTTCCCGCAG CCTTAGGAGT
    AGACAGGTGG CCCCTGCCAT TGAGAAGGTG CAGGCCAAGT TTGAACGCAC CTGCTGCTCC
    CCAAAGTACG GTTCTCCCAA ACTTCAGAGA AAGCCCCTCT CAAAAGCTGA CCAGCCAAAT
    AGCAGGACCT CACCAGGCAT ACCCCAGAAA GGGTTCAGCG AGTCAGCCTG GGCCCGCTCC
    ACCACCACGA GGGAGAGCCC TGTGCACACC ACCATCAATG ATGGCCTCTC CAGCCTCTTC
    AATATCATCG ACCACAGTCC CATCGGGGTG CGGGCTGGGA GCCGATCTCG GTCAGCAGAA
    CCTCGACAGG AGCTGGGTCC AGGCCAGGAA ACGGGCACCA GTTCCAGAGG GAGGTCGCCC
    AGCCCGCTAG GGGTAGGCTC CGAGACGTTC AGGGAGGAGG GAGGAGAGAG CACGCCAGTG
    AGACAGGACT TATCTGCGCC CCCTGGCTAC ACGCTCACTG AGAATGTGGC CCGGATCCTC
    AACAAAAAGT TGTTGGAGCA TGCCTTAAAG GAGGAGAGGA AGCAAGCCAG CCATGGCTCC
    TCAGGTCTCA CCAGCGACAG CCACACTGGA GAACCAGTGC CAGCCGAGCC AGGATCCATG
    GAGGAACTGC CTTGTTCTGC GCTCGCGCCC TCCCTAGAGC CCTGCTTTTC CAGGCCAGAG
    AGACCAGCAA ACCGCCGCCT TCCATCCCGT TGGGCCCCAC CTTCCCCCAC TGCCTCACAG
    TCACAGTCAC CCGGACACCC GATGTCCATG GAGGAACACG GTGAGGAGGA CCCACCAGAG
    GAGAAGCCAC ACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_766551.3
    CDS406..6243
    Translation

    Target ORF information:

    RefSeq Version NM_172963.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172963.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    ATGGAGGAGG ACCACCTCTA CGCCCTGCGG TGGAAGGAAC TGGAGATGCA CAGCCTGGCC
    CTGCAGAACA CCCTTCACAA GCGGACGTGG AGCGACGAGA AGAATCTGCT GCAGCAGGAG
    CTCAGGTCCC TGAAGCAGAA CATCTTCCTC TTCTACGTCA AGCTCAGGTG GCTGCTGAAA
    CACTGGCGCC AAGGGAAGCA GATGGAGGAG GGAGGAGAGG ATCTGGAGGA GAGCGAACAT
    CCAGAGAATG TGCCTGGGCT TGCTGAGCTT GGAGTCCAGG GGGTTCACCA GACAGATGGG
    ATAGACCAGG AGGATGCTGA CCAAGGCTGT AGCCTTCCCA TGGGAGAGCA TGCCCCACAC
    TCCCTGGTAC AGATCAGCGA ACACGGATCA CGGCTACAGA GTTCAGACGG GGGACCACTT
    AACAAACAGG TGGTGGAAAA CCAGCAGCTC TTCAGGGCCC TCAAGGCTCT GCTGGAGGAC
    TTCCGTTCGG AGCTGCGCGA AGACGAGCAT GCGCGGCTGC GGCTGCAGCA GCAGTACGCC
    AGCGACAAGG CTGCGTGGGA TGTGGAGTGG GCTGTGCTCA AGTGCCGCCT GGAACAGCTG
    GAAGAGAAGA CTGAGAAAAG CTTGGGCGAG CTGGACTCCT CTGCTGAGGG CAAAGGGGCC
    TTGAAGAAGG AGAGAGAGGT GCACCAGAAA CTGCTGGCTG ACAGCCACAG CCTGGTGATG
    GACCTGCGCT GGCAGATCCA TCACCGAGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTG
    CTGGAGCGTC TGGACAGCGA GCGGCAGGAG TGGGGGCGAC AGAAGGAGGA GCTCCTGTGG
    CGGGTGGAGC AGTTACAGAA AGAGAAAAGT CCCCGGAGAA GCGGCAGTTT CCTCTGCAGC
    CGAAGGGAAG ACGACACACG CCCTTACCCG CACCAAGGAA GCCTTCACTC CTCCAGACCT
    GTGTCCATGT GGCCCTGTGA AGACGCCGAC TCCATCCCAT TTGAAGACCG GCCGCTCTCC
    AAACTGAAGG AGTCGGACAG GTGCTCGGCC AGTGAGAACC TGTACTTGGA CGCTTTGTCC
    CTGGATGATG ATCCAGGGGA TCCGCCACCC CTCAGGAACT GCCTTGCCGA GGAAGAAGAA
    AGTCGCAAGG GCAATCTTCA GAGGGCTGTG TCTGTGTCCT GTATGTCCGA GTTCCAGCGT
    CTGATGGACG TCTCTCCGTT CCTGCCTGAG AAGGGGCTAC CTTCCGCCGG CAGCAAGGAG
    GATGTCACTC CACCCTTGTC TCCCGACGAC TTGAAATACA TTGAAGAATT CCATAGCAAC
    GACTGGGATT ACGCATCCCC CAGGGCTGAG GCCGATCGGC CTCCAGACCC CTGGGCAGAC
    AGGACCGAGA TGGGGAGGGT GGGGCATGAA GCCACCACAG AGCCTTGTCC GGATCCCTCC
    TGGTATCTGA CCACAAGTGT CACGATGACG ACAGACACCA TGACCAGTCC GGAGCACTGC
    CAGAAGCAGC CACTGCGGAC CCATGTCCTC ACTGAGCAGT CTGGTGTGCA CGTGCTGCAC
    AGCCCGCCTG CCATTCGCCG GGTTGACAGT ATAGCATCAG GCGGCGAGGG CAGGAGCCGA
    GCGGACCCCG AGGGCCCCTT TCCTATGAGC AGAGCAAGAG GGAACCTGGC AGATGCCAAG
    GGGGGTCATC CAGAACCTGT GCTCAACAGG TGGCCTTGCA CGCCACCCAG ACATCCCCGG
    GACTGTGTGG AGGGGTCTCT TCGCCCCCTC GACAGACCTA TCTGCCCCTC CCTGGGATTT
    GCCTCCCCTC TGAACAGCCT AGATATGTCT AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCTTTTTCTG TCAGGAATGC CATCTGCGCC GGTCCTGCCG AGCCACACGT CAGGGATATG
    GCCTGCCAGA CCAATGGGTC CAGGACAGCA GGGACGCAGA CCATCCAGAC AATCAGCGTG
    GGCCTGCAGA CCGAGGCCCT ACGTGCCAGC GGCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACGCCAAAAG CCGGAGGTGG CACCACACCC GTGTCTTCTC CTTCCCGCAG CCTTAGGAGT
    AGACAGGTGG CCCCTGCCAT TGAGAAGGTG CAGGCCAAGT TTGAACGCAC CTGCTGCTCC
    CCAAAGTACG GTTCTCCCAA ACTTCAGAGA AAGCCCCTCT CAAAAGCTGA CCAGCCAAAT
    AGCAGGACCT CACCAGGCAT ACCCCAGAAA GGGTTCAGCG AGTCAGCCTG GGCCCGCTCC
    ACCACCACGA GGGAGAGCCC TGTGCACACC ACCATCAATG ATGGCCTCTC CAGCCTCTTC
    AATATCATCG ACCACAGTCC CATCGGGGTG CGGGCTGGGA GCCGATCTCG GTCAGCAGAA
    CCTCGACAGG AGCTGGGTCC AGGCCAGGAA ACGGGCACCA GTTCCAGAGG GAGGTCGCCC
    AGCCCGCTAG GGGTAGGCTC CGAGACGTTC AGGGAGGAGG GAGGAGAGAG CACGCCAGTG
    AGACAGGACT TATCTGCGCC CCCTGGCTAC ACGCTCACTG AGAATGTGGC CCGGATCCTC
    AACAAAAAGT TGTTGGAGCA TGCCTTAAAG GAGGAGAGGA AGCAAGCCAG CCATGGCTCC
    TCAGGTCTCA CCAGCGACAG CCACACTGGA GAACCAGTGC CAGCCGAGCC AGGATCCATG
    GAGGAACTGC CTTGTTCTGC GCTCGCGCCC TCCCTAGAGC CCTGCTTTTC CAGGCCAGAG
    AGACCAGCAA ACCGCCGCCT TCCATCCCGT TGGGCCCCAC CTTCCCCCAC TGCCTCACAG
    TCACAGTCAC CCGGACACCC GATGTCCATG GAGGAACACG GTGAGGAGGA CCCACCAGAG
    GAGAAGCCAC ACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23364
    Clone ID Related Accession (Same CDS sequence) NM_001114098.1 , NM_001114098.2
    Accession Version NM_001114098.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-26
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147205.1, AK147631.1 and BP760436.1. Transcript Variant: This variant (1) represents the longer transcript but encodes the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK147631.1, AK147691.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    ATGGAGGAGG ACCACCTCTA CGCCCTGCGG TGGAAGGAAC TGGAGATGCA CAGCCTGGCC
    CTGCAGAACA CCCTTCACAA GCGGACGTGG AGCGACGAGA AGAATCTGCT GCAGCAGGAG
    CTCAGGTCCC TGAAGCAGAA CATCTTCCTC TTCTACGTCA AGCTCAGGTG GCTGCTGAAA
    CACTGGCGCC AAGGGAAGCA GATGGAGGAG GGAGGAGAGG ATCTGGAGGA GAGCGAACAT
    CCAGAGAATG TGCCTGGGCT TGCTGAGCTT GGAGTCCAGG GGGTTCACCA GACAGATGGG
    ATAGACCAGG AGGATGCTGA CCAAGGCTGT AGCCTTCCCA TGGGAGAGCA TGCCCCACAC
    TCCCTGGTAC AGATCAGCGA ACACGGATCA CGGCTACAGA GTTCAGACGG GGGACCACTT
    AACAAACAGG TGGTGGAAAA CCAGCAGCTC TTCAGGGCCC TCAAGGCTCT GCTGGAGGAC
    TTCCGTTCGG AGCTGCGCGA AGACGAGCAT GCGCGGCTGC GGCTGCAGCA GCAGTACGCC
    AGCGACAAGG CTGCGTGGGA TGTGGAGTGG GCTGTGCTCA AGTGCCGCCT GGAACAGCTG
    GAAGAGAAGA CTGAGAAAAG CTTGGGCGAG CTGGACTCCT CTGCTGAGGG CAAAGGGGCC
    TTGAAGAAGG AGAGAGAGGT GCACCAGAAA CTGCTGGCTG ACAGCCACAG CCTGGTGATG
    GACCTGCGCT GGCAGATCCA TCACCGAGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTG
    CTGGAGCGTC TGGACAGCGA GCGGCAGGAG TGGGGGCGAC AGAAGGAGGA GCTCCTGTGG
    CGGGTGGAGC AGTTACAGAA AGAGAAAAGT CCCCGGAGAA GCGGCAGTTT CCTCTGCAGC
    CGAAGGGAAG ACGACACACG CCCTTACCCG CACCAAGGAA GCCTTCACTC CTCCAGACCT
    GTGTCCATGT GGCCCTGTGA AGACGCCGAC TCCATCCCAT TTGAAGACCG GCCGCTCTCC
    AAACTGAAGG AGTCGGACAG GTGCTCGGCC AGTGAGAACC TGTACTTGGA CGCTTTGTCC
    CTGGATGATG ATCCAGGGGA TCCGCCACCC CTCAGGAACT GCCTTGCCGA GGAAGAAGAA
    AGTCGCAAGG GCAATCTTCA GAGGGCTGTG TCTGTGTCCT GTATGTCCGA GTTCCAGCGT
    CTGATGGACG TCTCTCCGTT CCTGCCTGAG AAGGGGCTAC CTTCCGCCGG CAGCAAGGAG
    GATGTCACTC CACCCTTGTC TCCCGACGAC TTGAAATACA TTGAAGAATT CCATAGCAAC
    GACTGGGATT ACGCATCCCC CAGGGCTGAG GCCGATCGGC CTCCAGACCC CTGGGCAGAC
    AGGACCGAGA TGGGGAGGGT GGGGCATGAA GCCACCACAG AGCCTTGTCC GGATCCCTCC
    TGGTATCTGA CCACAAGTGT CACGATGACG ACAGACACCA TGACCAGTCC GGAGCACTGC
    CAGAAGCAGC CACTGCGGAC CCATGTCCTC ACTGAGCAGT CTGGTGTGCA CGTGCTGCAC
    AGCCCGCCTG CCATTCGCCG GGTTGACAGT ATAGCATCAG GCGGCGAGGG CAGGAGCCGA
    GCGGACCCCG AGGGCCCCTT TCCTATGAGC AGAGCAAGAG GGAACCTGGC AGATGCCAAG
    GGGGGTCATC CAGAACCTGT GCTCAACAGG TGGCCTTGCA CGCCACCCAG ACATCCCCGG
    GACTGTGTGG AGGGGTCTCT TCGCCCCCTC GACAGACCTA TCTGCCCCTC CCTGGGATTT
    GCCTCCCCTC TGAACAGCCT AGATATGTCT AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCTTTTTCTG TCAGGAATGC CATCTGCGCC GGTCCTGCCG AGCCACACGT CAGGGATATG
    GCCTGCCAGA CCAATGGGTC CAGGACAGCA GGGACGCAGA CCATCCAGAC AATCAGCGTG
    GGCCTGCAGA CCGAGGCCCT ACGTGCCAGC GGCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACGCCAAAAG CCGGAGGTGG CACCACACCC GTGTCTTCTC CTTCCCGCAG CCTTAGGAGT
    AGACAGGTGG CCCCTGCCAT TGAGAAGGTG CAGGCCAAGT TTGAACGCAC CTGCTGCTCC
    CCAAAGTACG GTTCTCCCAA ACTTCAGAGA AAGCCCCTCT CAAAAGCTGA CCAGCCAAAT
    AGCAGGACCT CACCAGGCAT ACCCCAGAAA GGGTTCAGCG AGTCAGCCTG GGCCCGCTCC
    ACCACCACGA GGGAGAGCCC TGTGCACACC ACCATCAATG ATGGCCTCTC CAGCCTCTTC
    AATATCATCG ACCACAGTCC CATCGGGGTG CGGGCTGGGA GCCGATCTCG GTCAGCAGAA
    CCTCGACAGG AGCTGGGTCC AGGCCAGGAA ACGGGCACCA GTTCCAGAGG GAGGTCGCCC
    AGCCCGCTAG GGGTAGGCTC CGAGACGTTC AGGGAGGAGG GAGGAGAGAG CACGCCAGTG
    AGACAGGACT TATCTGCGCC CCCTGGCTAC ACGCTCACTG AGAATGTGGC CCGGATCCTC
    AACAAAAAGT TGTTGGAGCA TGCCTTAAAG GAGGAGAGGA AGCAAGCCAG CCATGGCTCC
    TCAGGTCTCA CCAGCGACAG CCACACTGGA GAACCAGTGC CAGCCGAGCC AGGATCCATG
    GAGAACCAAA CTGTCTTACT AACTGCCCCC TGGGGACTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107570.1
    CDS408..6089
    Translation

    Target ORF information:

    RefSeq Version NM_001114098.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001114098.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    ATGGAGGAGG ACCACCTCTA CGCCCTGCGG TGGAAGGAAC TGGAGATGCA CAGCCTGGCC
    CTGCAGAACA CCCTTCACAA GCGGACGTGG AGCGACGAGA AGAATCTGCT GCAGCAGGAG
    CTCAGGTCCC TGAAGCAGAA CATCTTCCTC TTCTACGTCA AGCTCAGGTG GCTGCTGAAA
    CACTGGCGCC AAGGGAAGCA GATGGAGGAG GGAGGAGAGG ATCTGGAGGA GAGCGAACAT
    CCAGAGAATG TGCCTGGGCT TGCTGAGCTT GGAGTCCAGG GGGTTCACCA GACAGATGGG
    ATAGACCAGG AGGATGCTGA CCAAGGCTGT AGCCTTCCCA TGGGAGAGCA TGCCCCACAC
    TCCCTGGTAC AGATCAGCGA ACACGGATCA CGGCTACAGA GTTCAGACGG GGGACCACTT
    AACAAACAGG TGGTGGAAAA CCAGCAGCTC TTCAGGGCCC TCAAGGCTCT GCTGGAGGAC
    TTCCGTTCGG AGCTGCGCGA AGACGAGCAT GCGCGGCTGC GGCTGCAGCA GCAGTACGCC
    AGCGACAAGG CTGCGTGGGA TGTGGAGTGG GCTGTGCTCA AGTGCCGCCT GGAACAGCTG
    GAAGAGAAGA CTGAGAAAAG CTTGGGCGAG CTGGACTCCT CTGCTGAGGG CAAAGGGGCC
    TTGAAGAAGG AGAGAGAGGT GCACCAGAAA CTGCTGGCTG ACAGCCACAG CCTGGTGATG
    GACCTGCGCT GGCAGATCCA TCACCGAGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTG
    CTGGAGCGTC TGGACAGCGA GCGGCAGGAG TGGGGGCGAC AGAAGGAGGA GCTCCTGTGG
    CGGGTGGAGC AGTTACAGAA AGAGAAAAGT CCCCGGAGAA GCGGCAGTTT CCTCTGCAGC
    CGAAGGGAAG ACGACACACG CCCTTACCCG CACCAAGGAA GCCTTCACTC CTCCAGACCT
    GTGTCCATGT GGCCCTGTGA AGACGCCGAC TCCATCCCAT TTGAAGACCG GCCGCTCTCC
    AAACTGAAGG AGTCGGACAG GTGCTCGGCC AGTGAGAACC TGTACTTGGA CGCTTTGTCC
    CTGGATGATG ATCCAGGGGA TCCGCCACCC CTCAGGAACT GCCTTGCCGA GGAAGAAGAA
    AGTCGCAAGG GCAATCTTCA GAGGGCTGTG TCTGTGTCCT GTATGTCCGA GTTCCAGCGT
    CTGATGGACG TCTCTCCGTT CCTGCCTGAG AAGGGGCTAC CTTCCGCCGG CAGCAAGGAG
    GATGTCACTC CACCCTTGTC TCCCGACGAC TTGAAATACA TTGAAGAATT CCATAGCAAC
    GACTGGGATT ACGCATCCCC CAGGGCTGAG GCCGATCGGC CTCCAGACCC CTGGGCAGAC
    AGGACCGAGA TGGGGAGGGT GGGGCATGAA GCCACCACAG AGCCTTGTCC GGATCCCTCC
    TGGTATCTGA CCACAAGTGT CACGATGACG ACAGACACCA TGACCAGTCC GGAGCACTGC
    CAGAAGCAGC CACTGCGGAC CCATGTCCTC ACTGAGCAGT CTGGTGTGCA CGTGCTGCAC
    AGCCCGCCTG CCATTCGCCG GGTTGACAGT ATAGCATCAG GCGGCGAGGG CAGGAGCCGA
    GCGGACCCCG AGGGCCCCTT TCCTATGAGC AGAGCAAGAG GGAACCTGGC AGATGCCAAG
    GGGGGTCATC CAGAACCTGT GCTCAACAGG TGGCCTTGCA CGCCACCCAG ACATCCCCGG
    GACTGTGTGG AGGGGTCTCT TCGCCCCCTC GACAGACCTA TCTGCCCCTC CCTGGGATTT
    GCCTCCCCTC TGAACAGCCT AGATATGTCT AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCTTTTTCTG TCAGGAATGC CATCTGCGCC GGTCCTGCCG AGCCACACGT CAGGGATATG
    GCCTGCCAGA CCAATGGGTC CAGGACAGCA GGGACGCAGA CCATCCAGAC AATCAGCGTG
    GGCCTGCAGA CCGAGGCCCT ACGTGCCAGC GGCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACGCCAAAAG CCGGAGGTGG CACCACACCC GTGTCTTCTC CTTCCCGCAG CCTTAGGAGT
    AGACAGGTGG CCCCTGCCAT TGAGAAGGTG CAGGCCAAGT TTGAACGCAC CTGCTGCTCC
    CCAAAGTACG GTTCTCCCAA ACTTCAGAGA AAGCCCCTCT CAAAAGCTGA CCAGCCAAAT
    AGCAGGACCT CACCAGGCAT ACCCCAGAAA GGGTTCAGCG AGTCAGCCTG GGCCCGCTCC
    ACCACCACGA GGGAGAGCCC TGTGCACACC ACCATCAATG ATGGCCTCTC CAGCCTCTTC
    AATATCATCG ACCACAGTCC CATCGGGGTG CGGGCTGGGA GCCGATCTCG GTCAGCAGAA
    CCTCGACAGG AGCTGGGTCC AGGCCAGGAA ACGGGCACCA GTTCCAGAGG GAGGTCGCCC
    AGCCCGCTAG GGGTAGGCTC CGAGACGTTC AGGGAGGAGG GAGGAGAGAG CACGCCAGTG
    AGACAGGACT TATCTGCGCC CCCTGGCTAC ACGCTCACTG AGAATGTGGC CCGGATCCTC
    AACAAAAAGT TGTTGGAGCA TGCCTTAAAG GAGGAGAGGA AGCAAGCCAG CCATGGCTCC
    TCAGGTCTCA CCAGCGACAG CCACACTGGA GAACCAGTGC CAGCCGAGCC AGGATCCATG
    GAGAACCAAA CTGTCTTACT AACTGCCCCC TGGGGACTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23364
    Clone ID Related Accession (Same CDS sequence) NM_001114098.1 , NM_001114098.2
    Accession Version NM_001114098.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT010496.14 and CT025601.7. On Aug 20, 2018 this sequence version replaced NM_001114098.1. Transcript Variant: This variant (1) represents the longer transcript but encodes the shorter isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK147691.1, AK147631.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    ATGGAGGAGG ACCACCTCTA CGCCCTGCGG TGGAAGGAAC TGGAGATGCA CAGCCTGGCC
    CTGCAGAACA CCCTTCACAA GCGGACGTGG AGCGACGAGA AGAATCTGCT GCAGCAGGAG
    CTCAGGTCCC TGAAGCAGAA CATCTTCCTC TTCTACGTCA AGCTCAGGTG GCTGCTGAAA
    CACTGGCGCC AAGGGAAGCA GATGGAGGAG GGAGGAGAGG ATCTGGAGGA GAGCGAACAT
    CCAGAGAATG TGCCTGGGCT TGCTGAGCTT GGAGTCCAGG GGGTTCACCA GACAGATGGG
    ATAGACCAGG AGGATGCTGA CCAAGGCTGT AGCCTTCCCA TGGGAGAGCA TGCCCCACAC
    TCCCTGGTAC AGATCAGCGA ACACGGATCA CGGCTACAGA GTTCAGACGG GGGACCACTT
    AACAAACAGG TGGTGGAAAA CCAGCAGCTC TTCAGGGCCC TCAAGGCTCT GCTGGAGGAC
    TTCCGTTCGG AGCTGCGCGA AGACGAGCAT GCGCGGCTGC GGCTGCAGCA GCAGTACGCC
    AGCGACAAGG CTGCGTGGGA TGTGGAGTGG GCTGTGCTCA AGTGCCGCCT GGAACAGCTG
    GAAGAGAAGA CTGAGAAAAG CTTGGGCGAG CTGGACTCCT CTGCTGAGGG CAAAGGGGCC
    TTGAAGAAGG AGAGAGAGGT GCACCAGAAA CTGCTGGCTG ACAGCCACAG CCTGGTGATG
    GACCTGCGCT GGCAGATCCA TCACCGAGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTG
    CTGGAGCGTC TGGACAGCGA GCGGCAGGAG TGGGGGCGAC AGAAGGAGGA GCTCCTGTGG
    CGGGTGGAGC AGTTACAGAA AGAGAAAAGT CCCCGGAGAA GCGGCAGTTT CCTCTGCAGC
    CGAAGGGAAG ACGACACACG CCCTTACCCG CACCAAGGAA GCCTTCACTC CTCCAGACCT
    GTGTCCATGT GGCCCTGTGA AGACGCCGAC TCCATCCCAT TTGAAGACCG GCCGCTCTCC
    AAACTGAAGG AGTCGGACAG GTGCTCGGCC AGTGAGAACC TGTACTTGGA CGCTTTGTCC
    CTGGATGATG ATCCAGGGGA TCCGCCACCC CTCAGGAACT GCCTTGCCGA GGAAGAAGAA
    AGTCGCAAGG GCAATCTTCA GAGGGCTGTG TCTGTGTCCT GTATGTCCGA GTTCCAGCGT
    CTGATGGACG TCTCTCCGTT CCTGCCTGAG AAGGGGCTAC CTTCCGCCGG CAGCAAGGAG
    GATGTCACTC CACCCTTGTC TCCCGACGAC TTGAAATACA TTGAAGAATT CCATAGCAAC
    GACTGGGATT ACGCATCCCC CAGGGCTGAG GCCGATCGGC CTCCAGACCC CTGGGCAGAC
    AGGACCGAGA TGGGGAGGGT GGGGCATGAA GCCACCACAG AGCCTTGTCC GGATCCCTCC
    TGGTATCTGA CCACAAGTGT CACGATGACG ACAGACACCA TGACCAGTCC GGAGCACTGC
    CAGAAGCAGC CACTGCGGAC CCATGTCCTC ACTGAGCAGT CTGGTGTGCA CGTGCTGCAC
    AGCCCGCCTG CCATTCGCCG GGTTGACAGT ATAGCATCAG GCGGCGAGGG CAGGAGCCGA
    GCGGACCCCG AGGGCCCCTT TCCTATGAGC AGAGCAAGAG GGAACCTGGC AGATGCCAAG
    GGGGGTCATC CAGAACCTGT GCTCAACAGG TGGCCTTGCA CGCCACCCAG ACATCCCCGG
    GACTGTGTGG AGGGGTCTCT TCGCCCCCTC GACAGACCTA TCTGCCCCTC CCTGGGATTT
    GCCTCCCCTC TGAACAGCCT AGATATGTCT AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCTTTTTCTG TCAGGAATGC CATCTGCGCC GGTCCTGCCG AGCCACACGT CAGGGATATG
    GCCTGCCAGA CCAATGGGTC CAGGACAGCA GGGACGCAGA CCATCCAGAC AATCAGCGTG
    GGCCTGCAGA CCGAGGCCCT ACGTGCCAGC GGCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACGCCAAAAG CCGGAGGTGG CACCACACCC GTGTCTTCTC CTTCCCGCAG CCTTAGGAGT
    AGACAGGTGG CCCCTGCCAT TGAGAAGGTG CAGGCCAAGT TTGAACGCAC CTGCTGCTCC
    CCAAAGTACG GTTCTCCCAA ACTTCAGAGA AAGCCCCTCT CAAAAGCTGA CCAGCCAAAT
    AGCAGGACCT CACCAGGCAT ACCCCAGAAA GGGTTCAGCG AGTCAGCCTG GGCCCGCTCC
    ACCACCACGA GGGAGAGCCC TGTGCACACC ACCATCAATG ATGGCCTCTC CAGCCTCTTC
    AATATCATCG ACCACAGTCC CATCGGGGTG CGGGCTGGGA GCCGATCTCG GTCAGCAGAA
    CCTCGACAGG AGCTGGGTCC AGGCCAGGAA ACGGGCACCA GTTCCAGAGG GAGGTCGCCC
    AGCCCGCTAG GGGTAGGCTC CGAGACGTTC AGGGAGGAGG GAGGAGAGAG CACGCCAGTG
    AGACAGGACT TATCTGCGCC CCCTGGCTAC ACGCTCACTG AGAATGTGGC CCGGATCCTC
    AACAAAAAGT TGTTGGAGCA TGCCTTAAAG GAGGAGAGGA AGCAAGCCAG CCATGGCTCC
    TCAGGTCTCA CCAGCGACAG CCACACTGGA GAACCAGTGC CAGCCGAGCC AGGATCCATG
    GAGAACCAAA CTGTCTTACT AACTGCCCCC TGGGGACTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107570.1
    CDS406..6087
    Translation

    Target ORF information:

    RefSeq Version NM_001114098.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001114098.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    ATGGAGGAGG ACCACCTCTA CGCCCTGCGG TGGAAGGAAC TGGAGATGCA CAGCCTGGCC
    CTGCAGAACA CCCTTCACAA GCGGACGTGG AGCGACGAGA AGAATCTGCT GCAGCAGGAG
    CTCAGGTCCC TGAAGCAGAA CATCTTCCTC TTCTACGTCA AGCTCAGGTG GCTGCTGAAA
    CACTGGCGCC AAGGGAAGCA GATGGAGGAG GGAGGAGAGG ATCTGGAGGA GAGCGAACAT
    CCAGAGAATG TGCCTGGGCT TGCTGAGCTT GGAGTCCAGG GGGTTCACCA GACAGATGGG
    ATAGACCAGG AGGATGCTGA CCAAGGCTGT AGCCTTCCCA TGGGAGAGCA TGCCCCACAC
    TCCCTGGTAC AGATCAGCGA ACACGGATCA CGGCTACAGA GTTCAGACGG GGGACCACTT
    AACAAACAGG TGGTGGAAAA CCAGCAGCTC TTCAGGGCCC TCAAGGCTCT GCTGGAGGAC
    TTCCGTTCGG AGCTGCGCGA AGACGAGCAT GCGCGGCTGC GGCTGCAGCA GCAGTACGCC
    AGCGACAAGG CTGCGTGGGA TGTGGAGTGG GCTGTGCTCA AGTGCCGCCT GGAACAGCTG
    GAAGAGAAGA CTGAGAAAAG CTTGGGCGAG CTGGACTCCT CTGCTGAGGG CAAAGGGGCC
    TTGAAGAAGG AGAGAGAGGT GCACCAGAAA CTGCTGGCTG ACAGCCACAG CCTGGTGATG
    GACCTGCGCT GGCAGATCCA TCACCGAGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTG
    CTGGAGCGTC TGGACAGCGA GCGGCAGGAG TGGGGGCGAC AGAAGGAGGA GCTCCTGTGG
    CGGGTGGAGC AGTTACAGAA AGAGAAAAGT CCCCGGAGAA GCGGCAGTTT CCTCTGCAGC
    CGAAGGGAAG ACGACACACG CCCTTACCCG CACCAAGGAA GCCTTCACTC CTCCAGACCT
    GTGTCCATGT GGCCCTGTGA AGACGCCGAC TCCATCCCAT TTGAAGACCG GCCGCTCTCC
    AAACTGAAGG AGTCGGACAG GTGCTCGGCC AGTGAGAACC TGTACTTGGA CGCTTTGTCC
    CTGGATGATG ATCCAGGGGA TCCGCCACCC CTCAGGAACT GCCTTGCCGA GGAAGAAGAA
    AGTCGCAAGG GCAATCTTCA GAGGGCTGTG TCTGTGTCCT GTATGTCCGA GTTCCAGCGT
    CTGATGGACG TCTCTCCGTT CCTGCCTGAG AAGGGGCTAC CTTCCGCCGG CAGCAAGGAG
    GATGTCACTC CACCCTTGTC TCCCGACGAC TTGAAATACA TTGAAGAATT CCATAGCAAC
    GACTGGGATT ACGCATCCCC CAGGGCTGAG GCCGATCGGC CTCCAGACCC CTGGGCAGAC
    AGGACCGAGA TGGGGAGGGT GGGGCATGAA GCCACCACAG AGCCTTGTCC GGATCCCTCC
    TGGTATCTGA CCACAAGTGT CACGATGACG ACAGACACCA TGACCAGTCC GGAGCACTGC
    CAGAAGCAGC CACTGCGGAC CCATGTCCTC ACTGAGCAGT CTGGTGTGCA CGTGCTGCAC
    AGCCCGCCTG CCATTCGCCG GGTTGACAGT ATAGCATCAG GCGGCGAGGG CAGGAGCCGA
    GCGGACCCCG AGGGCCCCTT TCCTATGAGC AGAGCAAGAG GGAACCTGGC AGATGCCAAG
    GGGGGTCATC CAGAACCTGT GCTCAACAGG TGGCCTTGCA CGCCACCCAG ACATCCCCGG
    GACTGTGTGG AGGGGTCTCT TCGCCCCCTC GACAGACCTA TCTGCCCCTC CCTGGGATTT
    GCCTCCCCTC TGAACAGCCT AGATATGTCT AAGAACATGA GTGATGACAT GAAGGAGGTG
    GCTTTTTCTG TCAGGAATGC CATCTGCGCC GGTCCTGCCG AGCCACACGT CAGGGATATG
    GCCTGCCAGA CCAATGGGTC CAGGACAGCA GGGACGCAGA CCATCCAGAC AATCAGCGTG
    GGCCTGCAGA CCGAGGCCCT ACGTGCCAGC GGCGTCACCA GCAGCCCCCA CAAGTGTCTC
    ACGCCAAAAG CCGGAGGTGG CACCACACCC GTGTCTTCTC CTTCCCGCAG CCTTAGGAGT
    AGACAGGTGG CCCCTGCCAT TGAGAAGGTG CAGGCCAAGT TTGAACGCAC CTGCTGCTCC
    CCAAAGTACG GTTCTCCCAA ACTTCAGAGA AAGCCCCTCT CAAAAGCTGA CCAGCCAAAT
    AGCAGGACCT CACCAGGCAT ACCCCAGAAA GGGTTCAGCG AGTCAGCCTG GGCCCGCTCC
    ACCACCACGA GGGAGAGCCC TGTGCACACC ACCATCAATG ATGGCCTCTC CAGCCTCTTC
    AATATCATCG ACCACAGTCC CATCGGGGTG CGGGCTGGGA GCCGATCTCG GTCAGCAGAA
    CCTCGACAGG AGCTGGGTCC AGGCCAGGAA ACGGGCACCA GTTCCAGAGG GAGGTCGCCC
    AGCCCGCTAG GGGTAGGCTC CGAGACGTTC AGGGAGGAGG GAGGAGAGAG CACGCCAGTG
    AGACAGGACT TATCTGCGCC CCCTGGCTAC ACGCTCACTG AGAATGTGGC CCGGATCCTC
    AACAAAAAGT TGTTGGAGCA TGCCTTAAAG GAGGAGAGGA AGCAAGCCAG CCATGGCTCC
    TCAGGTCTCA CCAGCGACAG CCACACTGGA GAACCAGTGC CAGCCGAGCC AGGATCCATG
    GAGAACCAAA CTGTCTTACT AACTGCCCCC TGGGGACTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53971
    Clone ID Related Accession (Same CDS sequence) XM_006524849.3 , XM_006524849.2
    Accession Version XM_006524849.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGCT CAGGGGCTCC
    CCAGTCTTAC CTGAGCAGAG TGTGTCTGTA GAAGAGCTAC AGGGTCAGCT CGAGCAGGCG
    GCCAGACTCC ATCAAGAGGA GACAGAGACA TACACAAACA AGATCCGTAA GATGGAGGAG
    GACCACCTCT ACGCCCTGCG GTGGAAGGAA CTGGAGATGC ACAGCCTGGC CCTGCAGAAC
    ACCCTTCACA AGCGGACGTG GAGCGACGAG AAGAATCTGC TGCAGCAGGA GCTCAGGTCC
    CTGAAGCAGA ACATCTTCCT CTTCTACGTC AAGCTCAGGT GGCTGCTGAA ACACTGGCGC
    CAAGGGAAGC AGATGGAGGA GGGAGGAGAG GATCTGGAGG AGAGCGAACA TCCAGAGAAT
    GTGCCTGGGC TTGCTGAGCT TGGAGTCCAG GGGGTTCACC AGACAGATGG GATAGACCAG
    GAGGATGCTG ACCAAGGCTG TAGCCTTCCC ATGGGAGAGC ATGCCCCACA CTCCCTGGTA
    CAGATCAGCG AACACGGATC ACGGCTACAG AGTTCAGACG GGGGACCACT TAACAAACAG
    GTGGTGGAAA ACCAGCAGCT CTTCAGGGCC CTCAAGGCTC TGCTGGAGGA CTTCCGTTCG
    GAGCTGCGCG AAGACGAGCA TGCGCGGCTG CGGCTGCAGC AGCAGTACGC CAGCGACAAG
    GCTGCGTGGG ATGTGGAGTG GGCTGTGCTC AAGTGCCGCC TGGAACAGCT GGAAGAGAAG
    ACTGAGAAAA GCTTGGGCGA GCTGGACTCC TCTGCTGAGG GCAAAGGGGC CTTGAAGAAG
    GAGAGAGAGG TGCACCAGAA ACTGCTGGCT GACAGCCACA GCCTGGTGAT GGACCTGCGC
    TGGCAGATCC ATCACCGAGA GAAGAACTGG AACCGGGAGA AGGTGGAGCT GCTGGAGCGT
    CTGGACAGCG AGCGGCAGGA GTGGGGGCGA CAGAAGGAGG AGCTCCTGTG GCGGGTGGAG
    CAGTTACAGA AAGAGAAAAG TCCCCGGAGA AGCGGCAGTT TCCTCTGCAG CCGAAGGGAA
    GACGACACAC GCCCTTACCC GCACCAAGGA AGCCTTCACT CCTCCAGACC TGTGTCCATG
    TGGCCCTGTG AAGACGCCGA CTCCATCCCA TTTGAAGACC GGCCGCTCTC CAAACTGAAG
    GAGTCGGACA GGTGCTCGGC CAGTGAGAAC CTGTACTTGG ACGCTTTGTC CCTGGATGAT
    GATCCAGGGG ATCCGCCACC CCTCAGGAAC TGCCTTGCCG AGGAAGAAGA AAGTCGCAAG
    GGCAATCTTC AGAGGGCTGT GTCTGTGTCC TGTATGTCCG AGTTCCAGCG TCTGATGGAC
    GTCTCTCCGT TCCTGCCTGA GAAGGGGCTA CCTTCCGCCG GCAGCAAGGA GGATGTCACT
    CCACCCTTGT CTCCCGACGA CTTGAAATAC ATTGAAGAAT TCCATAGCAA CGACTGGGAT
    TACGCATCCC CCAGGGCTGA GGCCGATCGG CCTCCAGACC CCTGGGCAGA CAGGACCGAG
    ATGGGGAGGG TGGGGCATGA AGCCACCACA GAGCCTTGTC CGGATCCCTC CTGGTATCTG
    ACCACAAGTG TCACGATGAC GACAGACACC ATGACCAGTC CGGAGCACTG CCAGAAGCAG
    CCACTGCGGA CCCATGTCCT CACTGAGCAG TCTGGTGTGC ACGTGCTGCA CAGCCCGCCT
    GCCATTCGCC GGGTTGACAG TATAGCATCA GGCGGCGAGG GCAGGAGCCG AGCGGACCCC
    GAGGGCCCCT TTCCTATGAG CAGAGCAAGA GGGAACCTGG CAGATGCCAA GGGGGGTCAT
    CCAGAACCTG TGCTCAACAG GTGGCCTTGC ACGCCACCCA GACATCCCCG GGACTGTGTG
    GAGGGGTCTC TTCGCCCCCT CGACAGACCT ATCTGCCCCT CCCTGGGATT TGCCTCCCCT
    CTGAACAGCC TAGATATGTC TAAGAACATG AGTGATGACA TGAAGGAGGT GGCTTTTTCT
    GTCAGGAATG CCATCTGCGC CGGTCCTGCC GAGCCACACG TCAGGGATAT GGCCTGCCAG
    ACCAATGGGT CCAGGACAGC AGGGACGCAG ACCATCCAGA CAATCAGCGT GGGCCTGCAG
    ACCGAGGCCC TACGTGCCAG CGGCGTCACC AGCAGCCCCC ACAAGTGTCT CACGCCAAAA
    GCCGGAGGTG GCACCACACC CGTGTCTTCT CCTTCCCGCA GCCTTAGGAG TAGACAGGTG
    GCCCCTGCCA TTGAGAAGGT GCAGGCCAAG TTTGAACGCA CCTGCTGCTC CCCAAAGTAC
    GGTTCTCCCA AACTTCAGAG AAAGCCCCTC TCAAAAGCTG ACCAGCCAAA TAGCAGGACC
    TCACCAGGCA TACCCCAGAA AGGGTTCAGC GAGTCAGCCT GGGCCCGCTC CACCACCACG
    AGGGAGAGCC CTGTGCACAC CACCATCAAT GATGGCCTCT CCAGCCTCTT CAATATCATC
    GACCACAGTC CCATCGGGGT GCGGGCTGGG AGCCGATCTC GGTCAGCAGA ACCTCGACAG
    GAGCTGGGTC CAGGCCAGGA AACGGGCACC AGTTCCAGAG GGAGGTCGCC CAGCCCGCTA
    GGGGTAGGCT CCGAGACGTT CAGGGAGGAG GGAGGAGAGA GCACGCCAGT GAGACAGGAC
    TTATCTGCGC CCCCTGGCTA CACGCTCACT GAGAATGTGG CCCGGATCCT CAACAAAAAG
    TTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AAGCAAGCCA GCCATGGCTC CTCAGGTCTC
    ACCAGCGACA GCCACACTGG AGAACCAGTG CCAGCCGAGC CAGGATCCAT GGAGGAACTG
    CCTTGTTCTG CGCTCGCGCC CTCCCTAGAG CCCTGCTTTT CCAGGCCAGA GAGACCAGCA
    AACCGCCGCC TTCCATCCCG TTGGGCCCCA CCTTCCCCCA CTGCCTCACA GTCACAGTCA
    CCCGGACACC CGATGTCCAT GGAGGAACAC GGTGAGGAGG ACCCACCAGA GGAGAAGCCA
    CACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524912.1
    CDS462..6530
    Translation

    Target ORF information:

    RefSeq Version XM_006524849.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524849.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGCT CAGGGGCTCC
    CCAGTCTTAC CTGAGCAGAG TGTGTCTGTA GAAGAGCTAC AGGGTCAGCT CGAGCAGGCG
    GCCAGACTCC ATCAAGAGGA GACAGAGACA TACACAAACA AGATCCGTAA GATGGAGGAG
    GACCACCTCT ACGCCCTGCG GTGGAAGGAA CTGGAGATGC ACAGCCTGGC CCTGCAGAAC
    ACCCTTCACA AGCGGACGTG GAGCGACGAG AAGAATCTGC TGCAGCAGGA GCTCAGGTCC
    CTGAAGCAGA ACATCTTCCT CTTCTACGTC AAGCTCAGGT GGCTGCTGAA ACACTGGCGC
    CAAGGGAAGC AGATGGAGGA GGGAGGAGAG GATCTGGAGG AGAGCGAACA TCCAGAGAAT
    GTGCCTGGGC TTGCTGAGCT TGGAGTCCAG GGGGTTCACC AGACAGATGG GATAGACCAG
    GAGGATGCTG ACCAAGGCTG TAGCCTTCCC ATGGGAGAGC ATGCCCCACA CTCCCTGGTA
    CAGATCAGCG AACACGGATC ACGGCTACAG AGTTCAGACG GGGGACCACT TAACAAACAG
    GTGGTGGAAA ACCAGCAGCT CTTCAGGGCC CTCAAGGCTC TGCTGGAGGA CTTCCGTTCG
    GAGCTGCGCG AAGACGAGCA TGCGCGGCTG CGGCTGCAGC AGCAGTACGC CAGCGACAAG
    GCTGCGTGGG ATGTGGAGTG GGCTGTGCTC AAGTGCCGCC TGGAACAGCT GGAAGAGAAG
    ACTGAGAAAA GCTTGGGCGA GCTGGACTCC TCTGCTGAGG GCAAAGGGGC CTTGAAGAAG
    GAGAGAGAGG TGCACCAGAA ACTGCTGGCT GACAGCCACA GCCTGGTGAT GGACCTGCGC
    TGGCAGATCC ATCACCGAGA GAAGAACTGG AACCGGGAGA AGGTGGAGCT GCTGGAGCGT
    CTGGACAGCG AGCGGCAGGA GTGGGGGCGA CAGAAGGAGG AGCTCCTGTG GCGGGTGGAG
    CAGTTACAGA AAGAGAAAAG TCCCCGGAGA AGCGGCAGTT TCCTCTGCAG CCGAAGGGAA
    GACGACACAC GCCCTTACCC GCACCAAGGA AGCCTTCACT CCTCCAGACC TGTGTCCATG
    TGGCCCTGTG AAGACGCCGA CTCCATCCCA TTTGAAGACC GGCCGCTCTC CAAACTGAAG
    GAGTCGGACA GGTGCTCGGC CAGTGAGAAC CTGTACTTGG ACGCTTTGTC CCTGGATGAT
    GATCCAGGGG ATCCGCCACC CCTCAGGAAC TGCCTTGCCG AGGAAGAAGA AAGTCGCAAG
    GGCAATCTTC AGAGGGCTGT GTCTGTGTCC TGTATGTCCG AGTTCCAGCG TCTGATGGAC
    GTCTCTCCGT TCCTGCCTGA GAAGGGGCTA CCTTCCGCCG GCAGCAAGGA GGATGTCACT
    CCACCCTTGT CTCCCGACGA CTTGAAATAC ATTGAAGAAT TCCATAGCAA CGACTGGGAT
    TACGCATCCC CCAGGGCTGA GGCCGATCGG CCTCCAGACC CCTGGGCAGA CAGGACCGAG
    ATGGGGAGGG TGGGGCATGA AGCCACCACA GAGCCTTGTC CGGATCCCTC CTGGTATCTG
    ACCACAAGTG TCACGATGAC GACAGACACC ATGACCAGTC CGGAGCACTG CCAGAAGCAG
    CCACTGCGGA CCCATGTCCT CACTGAGCAG TCTGGTGTGC ACGTGCTGCA CAGCCCGCCT
    GCCATTCGCC GGGTTGACAG TATAGCATCA GGCGGCGAGG GCAGGAGCCG AGCGGACCCC
    GAGGGCCCCT TTCCTATGAG CAGAGCAAGA GGGAACCTGG CAGATGCCAA GGGGGGTCAT
    CCAGAACCTG TGCTCAACAG GTGGCCTTGC ACGCCACCCA GACATCCCCG GGACTGTGTG
    GAGGGGTCTC TTCGCCCCCT CGACAGACCT ATCTGCCCCT CCCTGGGATT TGCCTCCCCT
    CTGAACAGCC TAGATATGTC TAAGAACATG AGTGATGACA TGAAGGAGGT GGCTTTTTCT
    GTCAGGAATG CCATCTGCGC CGGTCCTGCC GAGCCACACG TCAGGGATAT GGCCTGCCAG
    ACCAATGGGT CCAGGACAGC AGGGACGCAG ACCATCCAGA CAATCAGCGT GGGCCTGCAG
    ACCGAGGCCC TACGTGCCAG CGGCGTCACC AGCAGCCCCC ACAAGTGTCT CACGCCAAAA
    GCCGGAGGTG GCACCACACC CGTGTCTTCT CCTTCCCGCA GCCTTAGGAG TAGACAGGTG
    GCCCCTGCCA TTGAGAAGGT GCAGGCCAAG TTTGAACGCA CCTGCTGCTC CCCAAAGTAC
    GGTTCTCCCA AACTTCAGAG AAAGCCCCTC TCAAAAGCTG ACCAGCCAAA TAGCAGGACC
    TCACCAGGCA TACCCCAGAA AGGGTTCAGC GAGTCAGCCT GGGCCCGCTC CACCACCACG
    AGGGAGAGCC CTGTGCACAC CACCATCAAT GATGGCCTCT CCAGCCTCTT CAATATCATC
    GACCACAGTC CCATCGGGGT GCGGGCTGGG AGCCGATCTC GGTCAGCAGA ACCTCGACAG
    GAGCTGGGTC CAGGCCAGGA AACGGGCACC AGTTCCAGAG GGAGGTCGCC CAGCCCGCTA
    GGGGTAGGCT CCGAGACGTT CAGGGAGGAG GGAGGAGAGA GCACGCCAGT GAGACAGGAC
    TTATCTGCGC CCCCTGGCTA CACGCTCACT GAGAATGTGG CCCGGATCCT CAACAAAAAG
    TTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AAGCAAGCCA GCCATGGCTC CTCAGGTCTC
    ACCAGCGACA GCCACACTGG AGAACCAGTG CCAGCCGAGC CAGGATCCAT GGAGGAACTG
    CCTTGTTCTG CGCTCGCGCC CTCCCTAGAG CCCTGCTTTT CCAGGCCAGA GAGACCAGCA
    AACCGCCGCC TTCCATCCCG TTGGGCCCCA CCTTCCCCCA CTGCCTCACA GTCACAGTCA
    CCCGGACACC CGATGTCCAT GGAGGAACAC GGTGAGGAGG ACCCACCAGA GGAGAAGCCA
    CACCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53973
    Clone ID Related Accession (Same CDS sequence) XM_006524851.3 , XM_006524851.4
    Accession Version XM_006524851.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGATAACCC TCCCTCGGGC TATTCTTGCC
    TGGCAGGATG ACAGTGCGGA TTTGAAGTGT CAGCTTCAGT TTGTCAAAGA GGAAGCTTCC
    CTGATGCGCA AAAAGATGGC CAAGTTGGGG CGGGAGAAGG ACGAGTTGGA GCAGGAACTG
    CAGAAGTATA AGTCCCTCTA TGGTGATGTG GACAGTCCCC TCCCAACAGG GGAGGCTGGT
    GGTCCCCCCA GCACCAGGGA GGCAGAGCTG AAACTGCGGC TGAAGTTGGT GGAGGAGGAA
    GCCAGCATCT TGGGCAGGAA GATCGTGGAG CTGGAGGTGG AAAACCGTGG CCTCAAAGCA
    GAGATGGAAG ACATACGGGT TCAGCATGAA CGCGAGGGAA CAGGCAGGGA CCACGTGCCA
    AGCACTCCTA CCTCACCCTT CGGAGACTCA ATGGAGTCCT CCACGGAGCT CCGCCGGCAC
    CTGCAGTTTG TGGAAGAAGA GGCTGAGCTG CTGAGGAGAT CCATCTCTGA GATTGAAGAT
    CACAACCGGC AGTTGACCCA CGAGCTGAGC AAGTTCAAGT TTGAGCCTCA CCAGGAGTCA
    GGGTGGCTTG GGGACGGTGT GTCTAAGGGC CCCGCGGCCA GCGTTCCCCT GCAGGAGGAG
    CTGAAGTCAG CCAGGCTACA GATCGATGAG CTGAGCGGGA AGGTACTGAA GCTGCAGTGT
    GAGAACCGGC TGCTTCTGTC CAATGCCCAA CGGGGTGACC TCGCCGCACA TCTGGGGCTG
    CGCGCTCCTA GCCCCCGGGA CAGTGATGCC GAGAGTGACG CGGGCAAGAA AGAGAGCGAT
    GGGGAGGAGG GCCGCCTTCC CCAGCCAAAG CGAGAAGGCC CTGTTGGTGG GGAGAGCGAC
    TCGGAGGACA TGTTTGAGAA GACCTCGGGC TTCGGAAGCG GAAAGCCGTC GGAGGCTAGT
    GAGCCATGCC CGGCAGAGCT GCTAAGGGTC CGGGAGGATA CCGAGTGCCT GGTGACCATA
    AAGCTGGAGG CTCAGCGGCT TGAGCGGACT GTGGAGCGCC TCATCTCTGA TACAGATGGT
    TTCATCCACG ACTCAGGACT GCGGGGCAAT GGATTGGCCT CACCTGGAGT CCAAGGCGGA
    GGAGGTGAGG GCAACAGCCC GAGTGAGCCC CACTTGTTGG AGACCATCAA TGTGAGGATG
    AAGGCTTTCA GGAAAGAGCT GCAGGCCTTC CTGGAGCAGA TGTCCCGGAT TGTGGATGGT
    CTCTCGCCCT TATCCCACCT CACAGAGTCA TCCAGCTTCC TCTCCACTGT GACTTCTGTG
    TCCCGGGACT CCCCCATCGG GACCCTGGGA AAGGAGTTGG GCCCAGACTT GCAGTCCAAA
    CTGAGAGAGC AGCTGGAATG GCAGCTCAAT CAGGATCGAG GGGACGAGCG AGAGGGCCTG
    CGTCTCCGAG CCACTCGGGA GCTGCACCGG CGCGCGGATG GGGATTCCGG GAGCCACCAT
    GGGCTGGGAG GCCAGAGCTG TTTCAACCTA GAGCTCAGGG GCTCCCCAGT CTTACCTGAG
    CAGAGTGTGT CTGTAGAAGA GCTACAGGGT CAGCTCGAGC AGGCGGCCAG ACTCCATCAA
    GAGGAGACAG AGACATACAC AAACAAGATC CGTAAGATGG AGGAGGACCA CCTCTACGCC
    CTGCGGTGGA AGGAACTGGA GATGCACAGC CTGGCCCTGC AGAACACCCT TCACAAGCGG
    ACGTGGAGCG ACGAGAAGAA TCTGCTGCAG CAGGAGCTCA GGTCCCTGAA GCAGAACATC
    TTCCTCTTCT ACGTCAAGCT CAGGTGGCTG CTGAAACACT GGCGCCAAGG GAAGCAGATG
    GAGGAGGGAG GAGAGGATCT GGAGGAGAGC GAACATCCAG AGAATGTGCC TGGGCTTGCT
    GAGCTTGGAG TCCAGGGGGT TCACCAGACA GATGGGATAG ACCAGGAGGA TGCTGACCAA
    GGCTGTAGCC TTCCCATGGG AGAGCATGCC CCACACTCCC TGGTACAGAT CAGCGAACAC
    GGATCACGGC TACAGAGTTC AGACGGGGGA CCACTTAACA AACAGGTGGT GGAAAACCAG
    CAGCTCTTCA GGGCCCTCAA GGCTCTGCTG GAGGACTTCC GTTCGGAGCT GCGCGAAGAC
    GAGCATGCGC GGCTGCGGCT GCAGCAGCAG TACGCCAGCG ACAAGGCTGC GTGGGATGTG
    GAGTGGGCTG TGCTCAAGTG CCGCCTGGAA CAGCTGGAAG AGAAGACTGA GAAAAGCTTG
    GGCGAGCTGG ACTCCTCTGC TGAGGGCAAA GGGGCCTTGA AGAAGGAGAG AGAGGTGCAC
    CAGAAACTGC TGGCTGACAG CCACAGCCTG GTGATGGACC TGCGCTGGCA GATCCATCAC
    CGAGAGAAGA ACTGGAACCG GGAGAAGGTG GAGCTGCTGG AGCGTCTGGA CAGCGAGCGG
    CAGGAGTGGG GGCGACAGAA GGAGGAGCTC CTGTGGCGGG TGGAGCAGTT ACAGAAAGAG
    AAAAGTCCCC GGAGAAGCGG CAGTTTCCTC TGCAGCCGAA GGGAAGACGA CACACGCCCT
    TACCCGCACC AAGGAAGCCT TCACTCCTCC AGACCTGTGT CCATGTGGCC CTGTGAAGAC
    GCCGACTCCA TCCCATTTGA AGACCGGCCG CTCTCCAAAC TGAAGGAGTC GGACAGGTGC
    TCGGCCAGTG AGAACCTGTA CTTGGACGCT TTGTCCCTGG ATGATGATCC AGGGGATCCG
    CCACCCCTCA GGAACTGCCT TGCCGAGGAA GAAGAAAGTC GCAAGGGCAA TCTTCAGAGG
    GCTGTGTCTG TGTCCTGTAT GTCCGAGTTC CAGCGTCTGA TGGACGTCTC TCCGTTCCTG
    CCTGAGAAGG GGCTACCTTC CGCCGGCAGC AAGGAGGATG TCACTCCACC CTTGTCTCCC
    GACGACTTGA AATACATTGA AGAATTCCAT AGCAACGACT GGGATTACGC ATCCCCCAGG
    GCTGAGGCCG ATCGGCCTCC AGACCCCTGG GCAGACAGGA CCGAGATGGG GAGGGTGGGG
    CATGAAGCCA CCACAGAGCC TTGTCCGGAT CCCTCCTGGT ATCTGACCAC AAGTGTCACG
    ATGACGACAG ACACCATGAC CAGTCCGGAG CACTGCCAGA AGCAGCCACT GCGGACCCAT
    GTCCTCACTG AGCAGTCTGG TGTGCACGTG CTGCACAGCC CGCCTGCCAT TCGCCGGGTT
    GACAGTATAG CATCAGGCGG CGAGGGCAGG AGCCGAGCGG ACCCCGAGGG CCCCTTTCCT
    ATGAGCAGAG CAAGAGGGAA CCTGGCAGAT GCCAAGGGGG GTCATCCAGA ACCTGTGCTC
    AACAGGTGGC CTTGCACGCC ACCCAGACAT CCCCGGGACT GTGTGGAGGG GTCTCTTCGC
    CCCCTCGACA GACCTATCTG CCCCTCCCTG GGATTTGCCT CCCCTCTGAA CAGCCTAGAT
    ATGTCTAAGA ACATGAGTGA TGACATGAAG GAGGTGGCTT TTTCTGTCAG GAATGCCATC
    TGCGCCGGTC CTGCCGAGCC ACACGTCAGG GATATGGCCT GCCAGACCAA TGGGTCCAGG
    ACAGCAGGGA CGCAGACCAT CCAGACAATC AGCGTGGGCC TGCAGACCGA GGCCCTACGT
    GCCAGCGGCG TCACCAGCAG CCCCCACAAG TGTCTCACGC CAAAAGCCGG AGGTGGCACC
    ACACCCGTGT CTTCTCCTTC CCGCAGCCTT AGGAGTAGAC AGGTGGCCCC TGCCATTGAG
    AAGGTGCAGG CCAAGTTTGA ACGCACCTGC TGCTCCCCAA AGTACGGTTC TCCCAAACTT
    CAGAGAAAGC CCCTCTCAAA AGCTGACCAG CCAAATAGCA GGACCTCACC AGGCATACCC
    CAGAAAGGGT TCAGCGAGTC AGCCTGGGCC CGCTCCACCA CCACGAGGGA GAGCCCTGTG
    CACACCACCA TCAATGATGG CCTCTCCAGC CTCTTCAATA TCATCGACCA CAGTCCCATC
    GGGGTGCGGG CTGGGAGCCG ATCTCGGTCA GCAGAACCTC GACAGGAGCT GGGTCCAGGC
    CAGGAAACGG GCACCAGTTC CAGAGGGAGG TCGCCCAGCC CGCTAGGGGT AGGCTCCGAG
    ACGTTCAGGG AGGAGGGAGG AGAGAGCACG CCAGTGAGAC AGGACTTATC TGCGCCCCCT
    GGCTACACGC TCACTGAGAA TGTGGCCCGG ATCCTCAACA AAAAGTTGTT GGAGCATGCC
    TTAAAGGAGG AGAGGAAGCA AGCCAGCCAT GGCTCCTCAG GTCTCACCAG CGACAGCCAC
    ACTGGAGAAC CAGTGCCAGC CGAGCCAGGA TCCATGGAGG AACTGCCTTG TTCTGCGCTC
    GCGCCCTCCC TAGAGCCCTG CTTTTCCAGG CCAGAGAGAC CAGCAAACCG CCGCCTTCCA
    TCCCGTTGGG CCCCACCTTC CCCCACTGCC TCACAGTCAC AGTCACCCGG ACACCCGATG
    TCCATGGAGG AACACGGTGA GGAGGACCCA CCAGAGGAGA AGCCACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524914.1
    CDS461..6454
    Translation

    Target ORF information:

    RefSeq Version XM_006524851.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524851.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGATAACCC TCCCTCGGGC TATTCTTGCC
    TGGCAGGATG ACAGTGCGGA TTTGAAGTGT CAGCTTCAGT TTGTCAAAGA GGAAGCTTCC
    CTGATGCGCA AAAAGATGGC CAAGTTGGGG CGGGAGAAGG ACGAGTTGGA GCAGGAACTG
    CAGAAGTATA AGTCCCTCTA TGGTGATGTG GACAGTCCCC TCCCAACAGG GGAGGCTGGT
    GGTCCCCCCA GCACCAGGGA GGCAGAGCTG AAACTGCGGC TGAAGTTGGT GGAGGAGGAA
    GCCAGCATCT TGGGCAGGAA GATCGTGGAG CTGGAGGTGG AAAACCGTGG CCTCAAAGCA
    GAGATGGAAG ACATACGGGT TCAGCATGAA CGCGAGGGAA CAGGCAGGGA CCACGTGCCA
    AGCACTCCTA CCTCACCCTT CGGAGACTCA ATGGAGTCCT CCACGGAGCT CCGCCGGCAC
    CTGCAGTTTG TGGAAGAAGA GGCTGAGCTG CTGAGGAGAT CCATCTCTGA GATTGAAGAT
    CACAACCGGC AGTTGACCCA CGAGCTGAGC AAGTTCAAGT TTGAGCCTCA CCAGGAGTCA
    GGGTGGCTTG GGGACGGTGT GTCTAAGGGC CCCGCGGCCA GCGTTCCCCT GCAGGAGGAG
    CTGAAGTCAG CCAGGCTACA GATCGATGAG CTGAGCGGGA AGGTACTGAA GCTGCAGTGT
    GAGAACCGGC TGCTTCTGTC CAATGCCCAA CGGGGTGACC TCGCCGCACA TCTGGGGCTG
    CGCGCTCCTA GCCCCCGGGA CAGTGATGCC GAGAGTGACG CGGGCAAGAA AGAGAGCGAT
    GGGGAGGAGG GCCGCCTTCC CCAGCCAAAG CGAGAAGGCC CTGTTGGTGG GGAGAGCGAC
    TCGGAGGACA TGTTTGAGAA GACCTCGGGC TTCGGAAGCG GAAAGCCGTC GGAGGCTAGT
    GAGCCATGCC CGGCAGAGCT GCTAAGGGTC CGGGAGGATA CCGAGTGCCT GGTGACCATA
    AAGCTGGAGG CTCAGCGGCT TGAGCGGACT GTGGAGCGCC TCATCTCTGA TACAGATGGT
    TTCATCCACG ACTCAGGACT GCGGGGCAAT GGATTGGCCT CACCTGGAGT CCAAGGCGGA
    GGAGGTGAGG GCAACAGCCC GAGTGAGCCC CACTTGTTGG AGACCATCAA TGTGAGGATG
    AAGGCTTTCA GGAAAGAGCT GCAGGCCTTC CTGGAGCAGA TGTCCCGGAT TGTGGATGGT
    CTCTCGCCCT TATCCCACCT CACAGAGTCA TCCAGCTTCC TCTCCACTGT GACTTCTGTG
    TCCCGGGACT CCCCCATCGG GACCCTGGGA AAGGAGTTGG GCCCAGACTT GCAGTCCAAA
    CTGAGAGAGC AGCTGGAATG GCAGCTCAAT CAGGATCGAG GGGACGAGCG AGAGGGCCTG
    CGTCTCCGAG CCACTCGGGA GCTGCACCGG CGCGCGGATG GGGATTCCGG GAGCCACCAT
    GGGCTGGGAG GCCAGAGCTG TTTCAACCTA GAGCTCAGGG GCTCCCCAGT CTTACCTGAG
    CAGAGTGTGT CTGTAGAAGA GCTACAGGGT CAGCTCGAGC AGGCGGCCAG ACTCCATCAA
    GAGGAGACAG AGACATACAC AAACAAGATC CGTAAGATGG AGGAGGACCA CCTCTACGCC
    CTGCGGTGGA AGGAACTGGA GATGCACAGC CTGGCCCTGC AGAACACCCT TCACAAGCGG
    ACGTGGAGCG ACGAGAAGAA TCTGCTGCAG CAGGAGCTCA GGTCCCTGAA GCAGAACATC
    TTCCTCTTCT ACGTCAAGCT CAGGTGGCTG CTGAAACACT GGCGCCAAGG GAAGCAGATG
    GAGGAGGGAG GAGAGGATCT GGAGGAGAGC GAACATCCAG AGAATGTGCC TGGGCTTGCT
    GAGCTTGGAG TCCAGGGGGT TCACCAGACA GATGGGATAG ACCAGGAGGA TGCTGACCAA
    GGCTGTAGCC TTCCCATGGG AGAGCATGCC CCACACTCCC TGGTACAGAT CAGCGAACAC
    GGATCACGGC TACAGAGTTC AGACGGGGGA CCACTTAACA AACAGGTGGT GGAAAACCAG
    CAGCTCTTCA GGGCCCTCAA GGCTCTGCTG GAGGACTTCC GTTCGGAGCT GCGCGAAGAC
    GAGCATGCGC GGCTGCGGCT GCAGCAGCAG TACGCCAGCG ACAAGGCTGC GTGGGATGTG
    GAGTGGGCTG TGCTCAAGTG CCGCCTGGAA CAGCTGGAAG AGAAGACTGA GAAAAGCTTG
    GGCGAGCTGG ACTCCTCTGC TGAGGGCAAA GGGGCCTTGA AGAAGGAGAG AGAGGTGCAC
    CAGAAACTGC TGGCTGACAG CCACAGCCTG GTGATGGACC TGCGCTGGCA GATCCATCAC
    CGAGAGAAGA ACTGGAACCG GGAGAAGGTG GAGCTGCTGG AGCGTCTGGA CAGCGAGCGG
    CAGGAGTGGG GGCGACAGAA GGAGGAGCTC CTGTGGCGGG TGGAGCAGTT ACAGAAAGAG
    AAAAGTCCCC GGAGAAGCGG CAGTTTCCTC TGCAGCCGAA GGGAAGACGA CACACGCCCT
    TACCCGCACC AAGGAAGCCT TCACTCCTCC AGACCTGTGT CCATGTGGCC CTGTGAAGAC
    GCCGACTCCA TCCCATTTGA AGACCGGCCG CTCTCCAAAC TGAAGGAGTC GGACAGGTGC
    TCGGCCAGTG AGAACCTGTA CTTGGACGCT TTGTCCCTGG ATGATGATCC AGGGGATCCG
    CCACCCCTCA GGAACTGCCT TGCCGAGGAA GAAGAAAGTC GCAAGGGCAA TCTTCAGAGG
    GCTGTGTCTG TGTCCTGTAT GTCCGAGTTC CAGCGTCTGA TGGACGTCTC TCCGTTCCTG
    CCTGAGAAGG GGCTACCTTC CGCCGGCAGC AAGGAGGATG TCACTCCACC CTTGTCTCCC
    GACGACTTGA AATACATTGA AGAATTCCAT AGCAACGACT GGGATTACGC ATCCCCCAGG
    GCTGAGGCCG ATCGGCCTCC AGACCCCTGG GCAGACAGGA CCGAGATGGG GAGGGTGGGG
    CATGAAGCCA CCACAGAGCC TTGTCCGGAT CCCTCCTGGT ATCTGACCAC AAGTGTCACG
    ATGACGACAG ACACCATGAC CAGTCCGGAG CACTGCCAGA AGCAGCCACT GCGGACCCAT
    GTCCTCACTG AGCAGTCTGG TGTGCACGTG CTGCACAGCC CGCCTGCCAT TCGCCGGGTT
    GACAGTATAG CATCAGGCGG CGAGGGCAGG AGCCGAGCGG ACCCCGAGGG CCCCTTTCCT
    ATGAGCAGAG CAAGAGGGAA CCTGGCAGAT GCCAAGGGGG GTCATCCAGA ACCTGTGCTC
    AACAGGTGGC CTTGCACGCC ACCCAGACAT CCCCGGGACT GTGTGGAGGG GTCTCTTCGC
    CCCCTCGACA GACCTATCTG CCCCTCCCTG GGATTTGCCT CCCCTCTGAA CAGCCTAGAT
    ATGTCTAAGA ACATGAGTGA TGACATGAAG GAGGTGGCTT TTTCTGTCAG GAATGCCATC
    TGCGCCGGTC CTGCCGAGCC ACACGTCAGG GATATGGCCT GCCAGACCAA TGGGTCCAGG
    ACAGCAGGGA CGCAGACCAT CCAGACAATC AGCGTGGGCC TGCAGACCGA GGCCCTACGT
    GCCAGCGGCG TCACCAGCAG CCCCCACAAG TGTCTCACGC CAAAAGCCGG AGGTGGCACC
    ACACCCGTGT CTTCTCCTTC CCGCAGCCTT AGGAGTAGAC AGGTGGCCCC TGCCATTGAG
    AAGGTGCAGG CCAAGTTTGA ACGCACCTGC TGCTCCCCAA AGTACGGTTC TCCCAAACTT
    CAGAGAAAGC CCCTCTCAAA AGCTGACCAG CCAAATAGCA GGACCTCACC AGGCATACCC
    CAGAAAGGGT TCAGCGAGTC AGCCTGGGCC CGCTCCACCA CCACGAGGGA GAGCCCTGTG
    CACACCACCA TCAATGATGG CCTCTCCAGC CTCTTCAATA TCATCGACCA CAGTCCCATC
    GGGGTGCGGG CTGGGAGCCG ATCTCGGTCA GCAGAACCTC GACAGGAGCT GGGTCCAGGC
    CAGGAAACGG GCACCAGTTC CAGAGGGAGG TCGCCCAGCC CGCTAGGGGT AGGCTCCGAG
    ACGTTCAGGG AGGAGGGAGG AGAGAGCACG CCAGTGAGAC AGGACTTATC TGCGCCCCCT
    GGCTACACGC TCACTGAGAA TGTGGCCCGG ATCCTCAACA AAAAGTTGTT GGAGCATGCC
    TTAAAGGAGG AGAGGAAGCA AGCCAGCCAT GGCTCCTCAG GTCTCACCAG CGACAGCCAC
    ACTGGAGAAC CAGTGCCAGC CGAGCCAGGA TCCATGGAGG AACTGCCTTG TTCTGCGCTC
    GCGCCCTCCC TAGAGCCCTG CTTTTCCAGG CCAGAGAGAC CAGCAAACCG CCGCCTTCCA
    TCCCGTTGGG CCCCACCTTC CCCCACTGCC TCACAGTCAC AGTCACCCGG ACACCCGATG
    TCCATGGAGG AACACGGTGA GGAGGACCCA CCAGAGGAGA AGCCACACCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53974
    Clone ID Related Accession (Same CDS sequence) XM_006524852.3 , XM_006524852.4
    Accession Version XM_006524852.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    CTCAGGGGCT CCCCAGTCTT ACCTGAGCAG AGTGTGTCTG TAGAAGAGCT ACAGGGTCAG
    CTCGAGCAGG CGGCCAGACT CCATCAAGAG GAGACAGAGA CATACACAAA CAAGATCCGT
    AAGATGGAGG AGGACCACCT CTACGCCCTG CGGTGGAAGG AACTGGAGAT GCACAGCCTG
    GCCCTGCAGA ACACCCTTCA CAAGCGGACG TGGAGCGACG AGAAGAATCT GCTGCAGCAG
    GAGCTCAGGT CCCTGAAGCA GAACATCTTC CTCTTCTACG TCAAGCTCAG GTGGCTGCTG
    AAACACTGGC GCCAAGGGAA GCAGATGGAG GAGGGAGGAG AGGATCTGGA GGAGAGCGAA
    CATCCAGAGA ATGTGCCTGG GCTTGCTGAG CTTGGAGTCC AGGGGGTTCA CCAGACAGAT
    GGGATAGACC AGGAGGATGC TGACCAAGGC TGTAGCCTTC CCATGGGAGA GCATGCCCCA
    CACTCCCTGG TACAGATCAG CGAACACGGA TCACGGCTAC AGAGTTCAGA CGGGGGACCA
    CTTAACAAAC AGGTGGTGGA AAACCAGCAG CTCTTCAGGG CCCTCAAGGC TCTGCTGGAG
    GACTTCCGTT CGGAGCTGCG CGAAGACGAG CATGCGCGGC TGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AGGCTGCGTG GGATGTGGAG TGGGCTGTGC TCAAGTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA AAGCTTGGGC GAGCTGGACT CCTCTGCTGA GGGCAAAGGG
    GCCTTGAAGA AGGAGAGAGA GGTGCACCAG AAACTGCTGG CTGACAGCCA CAGCCTGGTG
    ATGGACCTGC GCTGGCAGAT CCATCACCGA GAGAAGAACT GGAACCGGGA GAAGGTGGAG
    CTGCTGGAGC GTCTGGACAG CGAGCGGCAG GAGTGGGGGC GACAGAAGGA GGAGCTCCTG
    TGGCGGGTGG AGCAGTTACA GAAAGAGAAA AGTCCCCGGA GAAGCGGCAG TTTCCTCTGC
    AGCCGAAGGG AAGACGACAC ACGCCCTTAC CCGCACCAAG GAAGCCTTCA CTCCTCCAGA
    CCTGTGTCCA TGTGGCCCTG TGAAGACGCC GACTCCATCC CATTTGAAGA CCGGCCGCTC
    TCCAAACTGA AGGAGTCGGA CAGGTGCTCG GCCAGTGAGA ACCTGTACTT GGACGCTTTG
    TCCCTGGATG ATGATCCAGG GGATCCGCCA CCCCTCAGGA ACTGCCTTGC CGAGGAAGAA
    GAAAGTCGCA AGGGCAATCT TCAGAGGGCT GTGTCTGTGT CCTGTATGTC CGAGTTCCAG
    CGTCTGATGG ACGTCTCTCC GTTCCTGCCT GAGAAGGGGC TACCTTCCGC CGGCAGCAAG
    GAGGATGTCA CTCCACCCTT GTCTCCCGAC GACTTGAAAT ACATTGAAGA ATTCCATAGC
    AACGACTGGG ATTACGCATC CCCCAGGGCT GAGGCCGATC GGCCTCCAGA CCCCTGGGCA
    GACAGGACCG AGATGGGGAG GGTGGGGCAT GAAGCCACCA CAGAGCCTTG TCCGGATCCC
    TCCTGGTATC TGACCACAAG TGTCACGATG ACGACAGACA CCATGACCAG TCCGGAGCAC
    TGCCAGAAGC AGCCACTGCG GACCCATGTC CTCACTGAGC AGTCTGGTGT GCACGTGCTG
    CACAGCCCGC CTGCCATTCG CCGGGTTGAC AGTATAGCAT CAGGCGGCGA GGGCAGGAGC
    CGAGCGGACC CCGAGGGCCC CTTTCCTATG AGCAGAGCAA GAGGGAACCT GGCAGATGCC
    AAGGGGGGTC ATCCAGAACC TGTGCTCAAC AGGTGGCCTT GCACGCCACC CAGACATCCC
    CGGGACTGTG TGGAGGGGTC TCTTCGCCCC CTCGACAGAC CTATCTGCCC CTCCCTGGGA
    TTTGCCTCCC CTCTGAACAG CCTAGATATG TCTAAGAACA TGAGTGATGA CATGAAGGAG
    GTGGCTTTTT CTGTCAGGAA TGCCATCTGC GCCGGTCCTG CCGAGCCACA CGTCAGGGAT
    ATGGCCTGCC AGACCAATGG GTCCAGGACA GCAGGGACGC AGACCATCCA GACAATCAGC
    GTGGGCCTGC AGACCGAGGC CCTACGTGCC AGCGGCGTCA CCAGCAGCCC CCACAAGTGT
    CTCACGCCAA AAGCCGGAGG TGGCACCACA CCCGTGTCTT CTCCTTCCCG CAGCCTTAGG
    AGTAGACAGG TGGCCCCTGC CATTGAGAAG GTGCAGGCCA AGTTTGAACG CACCTGCTGC
    TCCCCAAAGT ACGGTTCTCC CAAACTTCAG AGAAAGCCCC TCTCAAAAGC TGACCAGCCA
    AATAGCAGGA CCTCACCAGG CATACCCCAG AAAGGGTTCA GCGAGTCAGC CTGGGCCCGC
    TCCACCACCA CGAGGGAGAG CCCTGTGCAC ACCACCATCA ATGATGGCCT CTCCAGCCTC
    TTCAATATCA TCGACCACAG TCCCATCGGG GTGCGGGCTG GGAGCCGATC TCGGTCAGCA
    GAACCTCGAC AGGAGCTGGG TCCAGGCCAG GAAACGGGCA CCAGTTCCAG AGGGAGGTCG
    CCCAGCCCGC TAGGGGTAGG CTCCGAGACG TTCAGGGAGG AGGGAGGAGA GAGCACGCCA
    GTGAGACAGG ACTTATCTGC GCCCCCTGGC TACACGCTCA CTGAGAATGT GGCCCGGATC
    CTCAACAAAA AGTTGTTGGA GCATGCCTTA AAGGAGGAGA GGAAGCAAGC CAGCCATGGC
    TCCTCAGGTC TCACCAGCGA CAGCCACACT GGAGAACCAG TGCCAGCCGA GCCAGGATCC
    ATGGAGGAAC TGCCTTGTTC TGCGCTCGCG CCCTCCCTAG AGCCCTGCTT TTCCAGGCCA
    GAGAGACCAG CAAACCGCCG CCTTCCATCC CGTTGGGCCC CACCTTCCCC CACTGCCTCA
    CAGTCACAGT CACCCGGACA CCCGATGTCC ATGGAGGAAC ACGGTGAGGA GGACCCACCA
    GAGGAGAAGC CACACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524915.1
    CDS461..6421
    Translation

    Target ORF information:

    RefSeq Version XM_006524852.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524852.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    CTCAGGGGCT CCCCAGTCTT ACCTGAGCAG AGTGTGTCTG TAGAAGAGCT ACAGGGTCAG
    CTCGAGCAGG CGGCCAGACT CCATCAAGAG GAGACAGAGA CATACACAAA CAAGATCCGT
    AAGATGGAGG AGGACCACCT CTACGCCCTG CGGTGGAAGG AACTGGAGAT GCACAGCCTG
    GCCCTGCAGA ACACCCTTCA CAAGCGGACG TGGAGCGACG AGAAGAATCT GCTGCAGCAG
    GAGCTCAGGT CCCTGAAGCA GAACATCTTC CTCTTCTACG TCAAGCTCAG GTGGCTGCTG
    AAACACTGGC GCCAAGGGAA GCAGATGGAG GAGGGAGGAG AGGATCTGGA GGAGAGCGAA
    CATCCAGAGA ATGTGCCTGG GCTTGCTGAG CTTGGAGTCC AGGGGGTTCA CCAGACAGAT
    GGGATAGACC AGGAGGATGC TGACCAAGGC TGTAGCCTTC CCATGGGAGA GCATGCCCCA
    CACTCCCTGG TACAGATCAG CGAACACGGA TCACGGCTAC AGAGTTCAGA CGGGGGACCA
    CTTAACAAAC AGGTGGTGGA AAACCAGCAG CTCTTCAGGG CCCTCAAGGC TCTGCTGGAG
    GACTTCCGTT CGGAGCTGCG CGAAGACGAG CATGCGCGGC TGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AGGCTGCGTG GGATGTGGAG TGGGCTGTGC TCAAGTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA AAGCTTGGGC GAGCTGGACT CCTCTGCTGA GGGCAAAGGG
    GCCTTGAAGA AGGAGAGAGA GGTGCACCAG AAACTGCTGG CTGACAGCCA CAGCCTGGTG
    ATGGACCTGC GCTGGCAGAT CCATCACCGA GAGAAGAACT GGAACCGGGA GAAGGTGGAG
    CTGCTGGAGC GTCTGGACAG CGAGCGGCAG GAGTGGGGGC GACAGAAGGA GGAGCTCCTG
    TGGCGGGTGG AGCAGTTACA GAAAGAGAAA AGTCCCCGGA GAAGCGGCAG TTTCCTCTGC
    AGCCGAAGGG AAGACGACAC ACGCCCTTAC CCGCACCAAG GAAGCCTTCA CTCCTCCAGA
    CCTGTGTCCA TGTGGCCCTG TGAAGACGCC GACTCCATCC CATTTGAAGA CCGGCCGCTC
    TCCAAACTGA AGGAGTCGGA CAGGTGCTCG GCCAGTGAGA ACCTGTACTT GGACGCTTTG
    TCCCTGGATG ATGATCCAGG GGATCCGCCA CCCCTCAGGA ACTGCCTTGC CGAGGAAGAA
    GAAAGTCGCA AGGGCAATCT TCAGAGGGCT GTGTCTGTGT CCTGTATGTC CGAGTTCCAG
    CGTCTGATGG ACGTCTCTCC GTTCCTGCCT GAGAAGGGGC TACCTTCCGC CGGCAGCAAG
    GAGGATGTCA CTCCACCCTT GTCTCCCGAC GACTTGAAAT ACATTGAAGA ATTCCATAGC
    AACGACTGGG ATTACGCATC CCCCAGGGCT GAGGCCGATC GGCCTCCAGA CCCCTGGGCA
    GACAGGACCG AGATGGGGAG GGTGGGGCAT GAAGCCACCA CAGAGCCTTG TCCGGATCCC
    TCCTGGTATC TGACCACAAG TGTCACGATG ACGACAGACA CCATGACCAG TCCGGAGCAC
    TGCCAGAAGC AGCCACTGCG GACCCATGTC CTCACTGAGC AGTCTGGTGT GCACGTGCTG
    CACAGCCCGC CTGCCATTCG CCGGGTTGAC AGTATAGCAT CAGGCGGCGA GGGCAGGAGC
    CGAGCGGACC CCGAGGGCCC CTTTCCTATG AGCAGAGCAA GAGGGAACCT GGCAGATGCC
    AAGGGGGGTC ATCCAGAACC TGTGCTCAAC AGGTGGCCTT GCACGCCACC CAGACATCCC
    CGGGACTGTG TGGAGGGGTC TCTTCGCCCC CTCGACAGAC CTATCTGCCC CTCCCTGGGA
    TTTGCCTCCC CTCTGAACAG CCTAGATATG TCTAAGAACA TGAGTGATGA CATGAAGGAG
    GTGGCTTTTT CTGTCAGGAA TGCCATCTGC GCCGGTCCTG CCGAGCCACA CGTCAGGGAT
    ATGGCCTGCC AGACCAATGG GTCCAGGACA GCAGGGACGC AGACCATCCA GACAATCAGC
    GTGGGCCTGC AGACCGAGGC CCTACGTGCC AGCGGCGTCA CCAGCAGCCC CCACAAGTGT
    CTCACGCCAA AAGCCGGAGG TGGCACCACA CCCGTGTCTT CTCCTTCCCG CAGCCTTAGG
    AGTAGACAGG TGGCCCCTGC CATTGAGAAG GTGCAGGCCA AGTTTGAACG CACCTGCTGC
    TCCCCAAAGT ACGGTTCTCC CAAACTTCAG AGAAAGCCCC TCTCAAAAGC TGACCAGCCA
    AATAGCAGGA CCTCACCAGG CATACCCCAG AAAGGGTTCA GCGAGTCAGC CTGGGCCCGC
    TCCACCACCA CGAGGGAGAG CCCTGTGCAC ACCACCATCA ATGATGGCCT CTCCAGCCTC
    TTCAATATCA TCGACCACAG TCCCATCGGG GTGCGGGCTG GGAGCCGATC TCGGTCAGCA
    GAACCTCGAC AGGAGCTGGG TCCAGGCCAG GAAACGGGCA CCAGTTCCAG AGGGAGGTCG
    CCCAGCCCGC TAGGGGTAGG CTCCGAGACG TTCAGGGAGG AGGGAGGAGA GAGCACGCCA
    GTGAGACAGG ACTTATCTGC GCCCCCTGGC TACACGCTCA CTGAGAATGT GGCCCGGATC
    CTCAACAAAA AGTTGTTGGA GCATGCCTTA AAGGAGGAGA GGAAGCAAGC CAGCCATGGC
    TCCTCAGGTC TCACCAGCGA CAGCCACACT GGAGAACCAG TGCCAGCCGA GCCAGGATCC
    ATGGAGGAAC TGCCTTGTTC TGCGCTCGCG CCCTCCCTAG AGCCCTGCTT TTCCAGGCCA
    GAGAGACCAG CAAACCGCCG CCTTCCATCC CGTTGGGCCC CACCTTCCCC CACTGCCTCA
    CAGTCACAGT CACCCGGACA CCCGATGTCC ATGGAGGAAC ACGGTGAGGA GGACCCACCA
    GAGGAGAAGC CACACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53975
    Clone ID Related Accession (Same CDS sequence) XM_006524853.3 , XM_006524853.4
    Accession Version XM_006524853.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGAT GGAGGAGGAC
    CACCTCTACG CCCTGCGGTG GAAGGAACTG GAGATGCACA GCCTGGCCCT GCAGAACACC
    CTTCACAAGC GGACGTGGAG CGACGAGAAG AATCTGCTGC AGCAGGAGCT CAGGTCCCTG
    AAGCAGAACA TCTTCCTCTT CTACGTCAAG CTCAGGTGGC TGCTGAAACA CTGGCGCCAA
    GGGAAGCAGA TGGAGGAGGG AGGAGAGGAT CTGGAGGAGA GCGAACATCC AGAGAATGTG
    CCTGGGCTTG CTGAGCTTGG AGTCCAGGGG GTTCACCAGA CAGATGGGAT AGACCAGGAG
    GATGCTGACC AAGGCTGTAG CCTTCCCATG GGAGAGCATG CCCCACACTC CCTGGTACAG
    ATCAGCGAAC ACGGATCACG GCTACAGAGT TCAGACGGGG GACCACTTAA CAAACAGGTG
    GTGGAAAACC AGCAGCTCTT CAGGGCCCTC AAGGCTCTGC TGGAGGACTT CCGTTCGGAG
    CTGCGCGAAG ACGAGCATGC GCGGCTGCGG CTGCAGCAGC AGTACGCCAG CGACAAGGCT
    GCGTGGGATG TGGAGTGGGC TGTGCTCAAG TGCCGCCTGG AACAGCTGGA AGAGAAGACT
    GAGAAAAGCT TGGGCGAGCT GGACTCCTCT GCTGAGGGCA AAGGGGCCTT GAAGAAGGAG
    AGAGAGGTGC ACCAGAAACT GCTGGCTGAC AGCCACAGCC TGGTGATGGA CCTGCGCTGG
    CAGATCCATC ACCGAGAGAA GAACTGGAAC CGGGAGAAGG TGGAGCTGCT GGAGCGTCTG
    GACAGCGAGC GGCAGGAGTG GGGGCGACAG AAGGAGGAGC TCCTGTGGCG GGTGGAGCAG
    TTACAGAAAG AGAAAAGTCC CCGGAGAAGC GGCAGTTTCC TCTGCAGCCG AAGGGAAGAC
    GACACACGCC CTTACCCGCA CCAAGGAAGC CTTCACTCCT CCAGACCTGT GTCCATGTGG
    CCCTGTGAAG ACGCCGACTC CATCCCATTT GAAGACCGGC CGCTCTCCAA ACTGAAGGAG
    TCGGACAGGT GCTCGGCCAG TGAGAACCTG TACTTGGACG CTTTGTCCCT GGATGATGAT
    CCAGGGGATC CGCCACCCCT CAGGAACTGC CTTGCCGAGG AAGAAGAAAG TCGCAAGGGC
    AATCTTCAGA GGGCTGTGTC TGTGTCCTGT ATGTCCGAGT TCCAGCGTCT GATGGACGTC
    TCTCCGTTCC TGCCTGAGAA GGGGCTACCT TCCGCCGGCA GCAAGGAGGA TGTCACTCCA
    CCCTTGTCTC CCGACGACTT GAAATACATT GAAGAATTCC ATAGCAACGA CTGGGATTAC
    GCATCCCCCA GGGCTGAGGC CGATCGGCCT CCAGACCCCT GGGCAGACAG GACCGAGATG
    GGGAGGGTGG GGCATGAAGC CACCACAGAG CCTTGTCCGG ATCCCTCCTG GTATCTGACC
    ACAAGTGTCA CGATGACGAC AGACACCATG ACCAGTCCGG AGCACTGCCA GAAGCAGCCA
    CTGCGGACCC ATGTCCTCAC TGAGCAGTCT GGTGTGCACG TGCTGCACAG CCCGCCTGCC
    ATTCGCCGGG TTGACAGTAT AGCATCAGGC GGCGAGGGCA GGAGCCGAGC GGACCCCGAG
    GGCCCCTTTC CTATGAGCAG AGCAAGAGGG AACCTGGCAG ATGCCAAGGG GGGTCATCCA
    GAACCTGTGC TCAACAGGTG GCCTTGCACG CCACCCAGAC ATCCCCGGGA CTGTGTGGAG
    GGGTCTCTTC GCCCCCTCGA CAGACCTATC TGCCCCTCCC TGGGATTTGC CTCCCCTCTG
    AACAGCCTAG ATATGTCTAA GAACATGAGT GATGACATGA AGGAGGTGGC TTTTTCTGTC
    AGGAATGCCA TCTGCGCCGG TCCTGCCGAG CCACACGTCA GGGATATGGC CTGCCAGACC
    AATGGGTCCA GGACAGCAGG GACGCAGACC ATCCAGACAA TCAGCGTGGG CCTGCAGACC
    GAGGCCCTAC GTGCCAGCGG CGTCACCAGC AGCCCCCACA AGTGTCTCAC GCCAAAAGCC
    GGAGGTGGCA CCACACCCGT GTCTTCTCCT TCCCGCAGCC TTAGGAGTAG ACAGGTGGCC
    CCTGCCATTG AGAAGGTGCA GGCCAAGTTT GAACGCACCT GCTGCTCCCC AAAGTACGGT
    TCTCCCAAAC TTCAGAGAAA GCCCCTCTCA AAAGCTGACC AGCCAAATAG CAGGACCTCA
    CCAGGCATAC CCCAGAAAGG GTTCAGCGAG TCAGCCTGGG CCCGCTCCAC CACCACGAGG
    GAGAGCCCTG TGCACACCAC CATCAATGAT GGCCTCTCCA GCCTCTTCAA TATCATCGAC
    CACAGTCCCA TCGGGGTGCG GGCTGGGAGC CGATCTCGGT CAGCAGAACC TCGACAGGAG
    CTGGGTCCAG GCCAGGAAAC GGGCACCAGT TCCAGAGGGA GGTCGCCCAG CCCGCTAGGG
    GTAGGCTCCG AGACGTTCAG GGAGGAGGGA GGAGAGAGCA CGCCAGTGAG ACAGGACTTA
    TCTGCGCCCC CTGGCTACAC GCTCACTGAG AATGTGGCCC GGATCCTCAA CAAAAAGTTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAAG CAAGCCAGCC ATGGCTCCTC AGGTCTCACC
    AGCGACAGCC ACACTGGAGA ACCAGTGCCA GCCGAGCCAG GATCCATGGA GGAACTGCCT
    TGTTCTGCGC TCGCGCCCTC CCTAGAGCCC TGCTTTTCCA GGCCAGAGAG ACCAGCAAAC
    CGCCGCCTTC CATCCCGTTG GGCCCCACCT TCCCCCACTG CCTCACAGTC ACAGTCACCC
    GGACACCCGA TGTCCATGGA GGAACACGGT GAGGAGGACC CACCAGAGGA GAAGCCACAC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524916.1
    CDS461..6406
    Translation

    Target ORF information:

    RefSeq Version XM_006524853.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524853.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGAT GGAGGAGGAC
    CACCTCTACG CCCTGCGGTG GAAGGAACTG GAGATGCACA GCCTGGCCCT GCAGAACACC
    CTTCACAAGC GGACGTGGAG CGACGAGAAG AATCTGCTGC AGCAGGAGCT CAGGTCCCTG
    AAGCAGAACA TCTTCCTCTT CTACGTCAAG CTCAGGTGGC TGCTGAAACA CTGGCGCCAA
    GGGAAGCAGA TGGAGGAGGG AGGAGAGGAT CTGGAGGAGA GCGAACATCC AGAGAATGTG
    CCTGGGCTTG CTGAGCTTGG AGTCCAGGGG GTTCACCAGA CAGATGGGAT AGACCAGGAG
    GATGCTGACC AAGGCTGTAG CCTTCCCATG GGAGAGCATG CCCCACACTC CCTGGTACAG
    ATCAGCGAAC ACGGATCACG GCTACAGAGT TCAGACGGGG GACCACTTAA CAAACAGGTG
    GTGGAAAACC AGCAGCTCTT CAGGGCCCTC AAGGCTCTGC TGGAGGACTT CCGTTCGGAG
    CTGCGCGAAG ACGAGCATGC GCGGCTGCGG CTGCAGCAGC AGTACGCCAG CGACAAGGCT
    GCGTGGGATG TGGAGTGGGC TGTGCTCAAG TGCCGCCTGG AACAGCTGGA AGAGAAGACT
    GAGAAAAGCT TGGGCGAGCT GGACTCCTCT GCTGAGGGCA AAGGGGCCTT GAAGAAGGAG
    AGAGAGGTGC ACCAGAAACT GCTGGCTGAC AGCCACAGCC TGGTGATGGA CCTGCGCTGG
    CAGATCCATC ACCGAGAGAA GAACTGGAAC CGGGAGAAGG TGGAGCTGCT GGAGCGTCTG
    GACAGCGAGC GGCAGGAGTG GGGGCGACAG AAGGAGGAGC TCCTGTGGCG GGTGGAGCAG
    TTACAGAAAG AGAAAAGTCC CCGGAGAAGC GGCAGTTTCC TCTGCAGCCG AAGGGAAGAC
    GACACACGCC CTTACCCGCA CCAAGGAAGC CTTCACTCCT CCAGACCTGT GTCCATGTGG
    CCCTGTGAAG ACGCCGACTC CATCCCATTT GAAGACCGGC CGCTCTCCAA ACTGAAGGAG
    TCGGACAGGT GCTCGGCCAG TGAGAACCTG TACTTGGACG CTTTGTCCCT GGATGATGAT
    CCAGGGGATC CGCCACCCCT CAGGAACTGC CTTGCCGAGG AAGAAGAAAG TCGCAAGGGC
    AATCTTCAGA GGGCTGTGTC TGTGTCCTGT ATGTCCGAGT TCCAGCGTCT GATGGACGTC
    TCTCCGTTCC TGCCTGAGAA GGGGCTACCT TCCGCCGGCA GCAAGGAGGA TGTCACTCCA
    CCCTTGTCTC CCGACGACTT GAAATACATT GAAGAATTCC ATAGCAACGA CTGGGATTAC
    GCATCCCCCA GGGCTGAGGC CGATCGGCCT CCAGACCCCT GGGCAGACAG GACCGAGATG
    GGGAGGGTGG GGCATGAAGC CACCACAGAG CCTTGTCCGG ATCCCTCCTG GTATCTGACC
    ACAAGTGTCA CGATGACGAC AGACACCATG ACCAGTCCGG AGCACTGCCA GAAGCAGCCA
    CTGCGGACCC ATGTCCTCAC TGAGCAGTCT GGTGTGCACG TGCTGCACAG CCCGCCTGCC
    ATTCGCCGGG TTGACAGTAT AGCATCAGGC GGCGAGGGCA GGAGCCGAGC GGACCCCGAG
    GGCCCCTTTC CTATGAGCAG AGCAAGAGGG AACCTGGCAG ATGCCAAGGG GGGTCATCCA
    GAACCTGTGC TCAACAGGTG GCCTTGCACG CCACCCAGAC ATCCCCGGGA CTGTGTGGAG
    GGGTCTCTTC GCCCCCTCGA CAGACCTATC TGCCCCTCCC TGGGATTTGC CTCCCCTCTG
    AACAGCCTAG ATATGTCTAA GAACATGAGT GATGACATGA AGGAGGTGGC TTTTTCTGTC
    AGGAATGCCA TCTGCGCCGG TCCTGCCGAG CCACACGTCA GGGATATGGC CTGCCAGACC
    AATGGGTCCA GGACAGCAGG GACGCAGACC ATCCAGACAA TCAGCGTGGG CCTGCAGACC
    GAGGCCCTAC GTGCCAGCGG CGTCACCAGC AGCCCCCACA AGTGTCTCAC GCCAAAAGCC
    GGAGGTGGCA CCACACCCGT GTCTTCTCCT TCCCGCAGCC TTAGGAGTAG ACAGGTGGCC
    CCTGCCATTG AGAAGGTGCA GGCCAAGTTT GAACGCACCT GCTGCTCCCC AAAGTACGGT
    TCTCCCAAAC TTCAGAGAAA GCCCCTCTCA AAAGCTGACC AGCCAAATAG CAGGACCTCA
    CCAGGCATAC CCCAGAAAGG GTTCAGCGAG TCAGCCTGGG CCCGCTCCAC CACCACGAGG
    GAGAGCCCTG TGCACACCAC CATCAATGAT GGCCTCTCCA GCCTCTTCAA TATCATCGAC
    CACAGTCCCA TCGGGGTGCG GGCTGGGAGC CGATCTCGGT CAGCAGAACC TCGACAGGAG
    CTGGGTCCAG GCCAGGAAAC GGGCACCAGT TCCAGAGGGA GGTCGCCCAG CCCGCTAGGG
    GTAGGCTCCG AGACGTTCAG GGAGGAGGGA GGAGAGAGCA CGCCAGTGAG ACAGGACTTA
    TCTGCGCCCC CTGGCTACAC GCTCACTGAG AATGTGGCCC GGATCCTCAA CAAAAAGTTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAAG CAAGCCAGCC ATGGCTCCTC AGGTCTCACC
    AGCGACAGCC ACACTGGAGA ACCAGTGCCA GCCGAGCCAG GATCCATGGA GGAACTGCCT
    TGTTCTGCGC TCGCGCCCTC CCTAGAGCCC TGCTTTTCCA GGCCAGAGAG ACCAGCAAAC
    CGCCGCCTTC CATCCCGTTG GGCCCCACCT TCCCCCACTG CCTCACAGTC ACAGTCACCC
    GGACACCCGA TGTCCATGGA GGAACACGGT GAGGAGGACC CACCAGAGGA GAAGCCACAC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53976
    Clone ID Related Accession (Same CDS sequence) XM_006524856.3
    Accession Version XM_006524856.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGCT CAGGGGCTCC
    CCAGTCTTAC CTGAGCAGAG TGTGTCTGTA GAAGAGCTAC AGGGTCAGCT CGAGCAGGCG
    GCCAGACTCC ATCAAGAGGA GACAGAGACA TACACAAACA AGATCCGTAA GATGGAGGAG
    GACCACCTCT ACGCCCTGCG GTGGAAGGAA CTGGAGATGC ACAGCCTGGC CCTGCAGAAC
    ACCCTTCACA AGCGGACGTG GAGCGACGAG AAGAATCTGC TGCAGCAGGA GCTCAGGTCC
    CTGAAGCAGA ACATCTTCCT CTTCTACGTC AAGCTCAGGT GGCTGCTGAA ACACTGGCGC
    CAAGGGAAGC AGATGGAGGA GGGAGGAGAG GATCTGGAGG AGAGCGAACA TCCAGAGAAT
    GTGCCTGGGC TTGCTGAGCT TGGAGTCCAG GGGGTTCACC AGACAGATGG GATAGACCAG
    GAGGATGCTG ACCAAGGCTG TAGCCTTCCC ATGGGAGAGC ATGCCCCACA CTCCCTGGTA
    CAGATCAGCG AACACGGATC ACGGCTACAG AGTTCAGACG GGGGACCACT TAACAAACAG
    GTGGTGGAAA ACCAGCAGCT CTTCAGGGCC CTCAAGGCTC TGCTGGAGGA CTTCCGTTCG
    GAGCTGCGCG AAGACGAGCA TGCGCGGCTG CGGCTGCAGC AGCAGTACGC CAGCGACAAG
    GCTGCGTGGG ATGTGGAGTG GGCTGTGCTC AAGTGCCGCC TGGAACAGCT GGAAGAGAAG
    ACTGAGAAAA GCTTGGGCGA GCTGGACTCC TCTGCTGAGG GCAAAGGGGC CTTGAAGAAG
    GAGAGAGAGG TGCACCAGAA ACTGCTGGCT GACAGCCACA GCCTGGTGAT GGACCTGCGC
    TGGCAGATCC ATCACCGAGA GAAGAACTGG AACCGGGAGA AGGTGGAGCT GCTGGAGCGT
    CTGGACAGCG AGCGGCAGGA GTGGGGGCGA CAGAAGGAGG AGCTCCTGTG GCGGGTGGAG
    CAGTTACAGA AAGAGAAAAG TCCCCGGAGA AGCGGCAGTT TCCTCTGCAG CCGAAGGGAA
    GACGACACAC GCCCTTACCC GCACCAAGGA AGCCTTCACT CCTCCAGACC TGTGTCCATG
    TGGCCCTGTG AAGACGCCGA CTCCATCCCA TTTGAAGACC GGCCGCTCTC CAAACTGAAG
    GAGTCGGACA GGTGCTCGGC CAGTGAGAAC CTGTACTTGG ACGCTTTGTC CCTGGATGAT
    GATCCAGGGG ATCCGCCACC CCTCAGGAAC TGCCTTGCCG AGGAAGAAGA AAGTCGCAAG
    GGCAATCTTC AGAGGGCTGT GTCTGTGTCC TGTATGTCCG AGTTCCAGCG TCTGATGGAC
    GTCTCTCCGT TCCTGCCTGA GAAGGGGCTA CCTTCCGCCG GCAGCAAGGA GGATGTCACT
    CCACCCTTGT CTCCCGACGA CTTGAAATAC ATTGAAGAAT TCCATAGCAA CGACTGGGAT
    TACGCATCCC CCAGGGCTGA GGCCGATCGG CCTCCAGACC CCTGGGCAGA CAGGACCGAG
    ATGGGGAGGG TGGGGCATGA AGCCACCACA GAGCCTTGTC CGGATCCCTC CTGGTATCTG
    ACCACAAGTG TCACGATGAC GACAGACACC ATGACCAGTC CGGAGCACTG CCAGAAGCAG
    CCACTGCGGA CCCATGTCCT CACTGAGCAG TCTGGTGTGC ACGTGCTGCA CAGCCCGCCT
    GCCATTCGCC GGGTTGACAG TATAGCATCA GGCGGCGAGG GCAGGAGCCG AGCGGACCCC
    GAGGGCCCCT TTCCTATGAG CAGAGCAAGA GGGAACCTGG CAGATGCCAA GGGGGGTCAT
    CCAGAACCTG TGCTCAACAG GTGGCCTTGC ACGCCACCCA GACATCCCCG GGACTGTGTG
    GAGGGGTCTC TTCGCCCCCT CGACAGACCT ATCTGCCCCT CCCTGGGATT TGCCTCCCCT
    CTGAACAGCC TAGATATGTC TAAGAACATG AGTGATGACA TGAAGGAGGT GGCTTTTTCT
    GTCAGGAATG CCATCTGCGC CGGTCCTGCC GAGCCACACG TCAGGGATAT GGCCTGCCAG
    ACCAATGGGT CCAGGACAGC AGGGACGCAG ACCATCCAGA CAATCAGCGT GGGCCTGCAG
    ACCGAGGCCC TACGTGCCAG CGGCGTCACC AGCAGCCCCC ACAAGTGTCT CACGCCAAAA
    GCCGGAGGTG GCACCACACC CGTGTCTTCT CCTTCCCGCA GCCTTAGGAG TAGACAGGTG
    GCCCCTGCCA TTGAGAAGGT GCAGGCCAAG TTTGAACGCA CCTGCTGCTC CCCAAAGTAC
    GGTTCTCCCA AACTTCAGAG AAAGCCCCTC TCAAAAGCTG ACCAGCCAAA TAGCAGGACC
    TCACCAGGCA TACCCCAGAA AGGGTTCAGC GAGTCAGCCT GGGCCCGCTC CACCACCACG
    AGGGAGAGCC CTGTGCACAC CACCATCAAT GATGGCCTCT CCAGCCTCTT CAATATCATC
    GACCACAGTC CCATCGGGGT GCGGGCTGGG AGCCGATCTC GGTCAGCAGA ACCTCGACAG
    GAGCTGGGTC CAGGCCAGGA AACGGGCACC AGTTCCAGAG GGAGGTCGCC CAGCCCGCTA
    GGGGTAGGCT CCGAGACGTT CAGGGAGGAG GGAGGAGAGA GCACGCCAGT GAGACAGGAC
    TTATCTGCGC CCCCTGGCTA CACGCTCACT GAGAATGTGG CCCGGATCCT CAACAAAAAG
    TTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AAGCAAGCCA GCCATGGCTC CTCAGGTCTC
    ACCAGCGACA GCCACACTGG AGAACCAGTG CCAGCCGAGC CAGGATCCAT GGAGAACCAA
    ACTGTCTTAC TAACTGCCCC CTGGGGACTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524919.1
    CDS462..6374
    Translation

    Target ORF information:

    RefSeq Version XM_006524856.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524856.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGCT CAGGGGCTCC
    CCAGTCTTAC CTGAGCAGAG TGTGTCTGTA GAAGAGCTAC AGGGTCAGCT CGAGCAGGCG
    GCCAGACTCC ATCAAGAGGA GACAGAGACA TACACAAACA AGATCCGTAA GATGGAGGAG
    GACCACCTCT ACGCCCTGCG GTGGAAGGAA CTGGAGATGC ACAGCCTGGC CCTGCAGAAC
    ACCCTTCACA AGCGGACGTG GAGCGACGAG AAGAATCTGC TGCAGCAGGA GCTCAGGTCC
    CTGAAGCAGA ACATCTTCCT CTTCTACGTC AAGCTCAGGT GGCTGCTGAA ACACTGGCGC
    CAAGGGAAGC AGATGGAGGA GGGAGGAGAG GATCTGGAGG AGAGCGAACA TCCAGAGAAT
    GTGCCTGGGC TTGCTGAGCT TGGAGTCCAG GGGGTTCACC AGACAGATGG GATAGACCAG
    GAGGATGCTG ACCAAGGCTG TAGCCTTCCC ATGGGAGAGC ATGCCCCACA CTCCCTGGTA
    CAGATCAGCG AACACGGATC ACGGCTACAG AGTTCAGACG GGGGACCACT TAACAAACAG
    GTGGTGGAAA ACCAGCAGCT CTTCAGGGCC CTCAAGGCTC TGCTGGAGGA CTTCCGTTCG
    GAGCTGCGCG AAGACGAGCA TGCGCGGCTG CGGCTGCAGC AGCAGTACGC CAGCGACAAG
    GCTGCGTGGG ATGTGGAGTG GGCTGTGCTC AAGTGCCGCC TGGAACAGCT GGAAGAGAAG
    ACTGAGAAAA GCTTGGGCGA GCTGGACTCC TCTGCTGAGG GCAAAGGGGC CTTGAAGAAG
    GAGAGAGAGG TGCACCAGAA ACTGCTGGCT GACAGCCACA GCCTGGTGAT GGACCTGCGC
    TGGCAGATCC ATCACCGAGA GAAGAACTGG AACCGGGAGA AGGTGGAGCT GCTGGAGCGT
    CTGGACAGCG AGCGGCAGGA GTGGGGGCGA CAGAAGGAGG AGCTCCTGTG GCGGGTGGAG
    CAGTTACAGA AAGAGAAAAG TCCCCGGAGA AGCGGCAGTT TCCTCTGCAG CCGAAGGGAA
    GACGACACAC GCCCTTACCC GCACCAAGGA AGCCTTCACT CCTCCAGACC TGTGTCCATG
    TGGCCCTGTG AAGACGCCGA CTCCATCCCA TTTGAAGACC GGCCGCTCTC CAAACTGAAG
    GAGTCGGACA GGTGCTCGGC CAGTGAGAAC CTGTACTTGG ACGCTTTGTC CCTGGATGAT
    GATCCAGGGG ATCCGCCACC CCTCAGGAAC TGCCTTGCCG AGGAAGAAGA AAGTCGCAAG
    GGCAATCTTC AGAGGGCTGT GTCTGTGTCC TGTATGTCCG AGTTCCAGCG TCTGATGGAC
    GTCTCTCCGT TCCTGCCTGA GAAGGGGCTA CCTTCCGCCG GCAGCAAGGA GGATGTCACT
    CCACCCTTGT CTCCCGACGA CTTGAAATAC ATTGAAGAAT TCCATAGCAA CGACTGGGAT
    TACGCATCCC CCAGGGCTGA GGCCGATCGG CCTCCAGACC CCTGGGCAGA CAGGACCGAG
    ATGGGGAGGG TGGGGCATGA AGCCACCACA GAGCCTTGTC CGGATCCCTC CTGGTATCTG
    ACCACAAGTG TCACGATGAC GACAGACACC ATGACCAGTC CGGAGCACTG CCAGAAGCAG
    CCACTGCGGA CCCATGTCCT CACTGAGCAG TCTGGTGTGC ACGTGCTGCA CAGCCCGCCT
    GCCATTCGCC GGGTTGACAG TATAGCATCA GGCGGCGAGG GCAGGAGCCG AGCGGACCCC
    GAGGGCCCCT TTCCTATGAG CAGAGCAAGA GGGAACCTGG CAGATGCCAA GGGGGGTCAT
    CCAGAACCTG TGCTCAACAG GTGGCCTTGC ACGCCACCCA GACATCCCCG GGACTGTGTG
    GAGGGGTCTC TTCGCCCCCT CGACAGACCT ATCTGCCCCT CCCTGGGATT TGCCTCCCCT
    CTGAACAGCC TAGATATGTC TAAGAACATG AGTGATGACA TGAAGGAGGT GGCTTTTTCT
    GTCAGGAATG CCATCTGCGC CGGTCCTGCC GAGCCACACG TCAGGGATAT GGCCTGCCAG
    ACCAATGGGT CCAGGACAGC AGGGACGCAG ACCATCCAGA CAATCAGCGT GGGCCTGCAG
    ACCGAGGCCC TACGTGCCAG CGGCGTCACC AGCAGCCCCC ACAAGTGTCT CACGCCAAAA
    GCCGGAGGTG GCACCACACC CGTGTCTTCT CCTTCCCGCA GCCTTAGGAG TAGACAGGTG
    GCCCCTGCCA TTGAGAAGGT GCAGGCCAAG TTTGAACGCA CCTGCTGCTC CCCAAAGTAC
    GGTTCTCCCA AACTTCAGAG AAAGCCCCTC TCAAAAGCTG ACCAGCCAAA TAGCAGGACC
    TCACCAGGCA TACCCCAGAA AGGGTTCAGC GAGTCAGCCT GGGCCCGCTC CACCACCACG
    AGGGAGAGCC CTGTGCACAC CACCATCAAT GATGGCCTCT CCAGCCTCTT CAATATCATC
    GACCACAGTC CCATCGGGGT GCGGGCTGGG AGCCGATCTC GGTCAGCAGA ACCTCGACAG
    GAGCTGGGTC CAGGCCAGGA AACGGGCACC AGTTCCAGAG GGAGGTCGCC CAGCCCGCTA
    GGGGTAGGCT CCGAGACGTT CAGGGAGGAG GGAGGAGAGA GCACGCCAGT GAGACAGGAC
    TTATCTGCGC CCCCTGGCTA CACGCTCACT GAGAATGTGG CCCGGATCCT CAACAAAAAG
    TTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AAGCAAGCCA GCCATGGCTC CTCAGGTCTC
    ACCAGCGACA GCCACACTGG AGAACCAGTG CCAGCCGAGC CAGGATCCAT GGAGAACCAA
    ACTGTCTTAC TAACTGCCCC CTGGGGACTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu70286
    Clone ID Related Accession (Same CDS sequence) XM_011246587.2 , XM_011246587.3
    Accession Version XM_011246587.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    CTCAGGGGCT CCCCAGTCTT ACCTGAGCAG AGTGTGTCTG TAGAAGAGCT ACAGGGTCAG
    CTCGAGCAGG CGGCCAGACT CCATCAAGAG GAGACAGAGA CATACACAAA CAAGATCCGT
    AAGATGGAGG AGGACCACCT CTACGCCCTG CGGTGGAAGG AACTGGAGAT GCACAGCCTG
    GCCCTGCAGA ACACCCTTCA CAAGCGGACG TGGAGCGACG AGAAGAATCT GCTGCAGCAG
    GAGCTCAGGT CCCTGAAGCA GAACATCTTC CTCTTCTACG TCAAGCTCAG GTGGCTGCTG
    AAACACTGGC GCCAAGGGAA GCAGATGGAG GAGGGAGGAG AGGATCTGGA GGAGAGCGAA
    CATCCAGAGA ATGTGCCTGG GCTTGCTGAG CTTGGAGTCC AGGGGGTTCA CCAGACAGAT
    GGGATAGACC AGGAGGATGC TGACCAAGGC TGTAGCCTTC CCATGGGAGA GCATGCCCCA
    CACTCCCTGG TACAGATCAG CGAACACGGA TCACGGCTAC AGAGTTCAGA CGGGGGACCA
    CTTAACAAAC AGGTGGTGGA AAACCAGCAG CTCTTCAGGG CCCTCAAGGC TCTGCTGGAG
    GACTTCCGTT CGGAGCTGCG CGAAGACGAG CATGCGCGGC TGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AGGCTGCGTG GGATGTGGAG TGGGCTGTGC TCAAGTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA AAGCTTGGGC GAGCTGGACT CCTCTGCTGA GGGCAAAGGG
    GCCTTGAAGA AGGAGAGAGA GGTGCACCAG AAACTGCTGG CTGACAGCCA CAGCCTGGTG
    ATGGACCTGC GCTGGCAGAT CCATCACCGA GAGAAGAACT GGAACCGGGA GAAGGTGGAG
    CTGCTGGAGC GTCTGGACAG CGAGCGGCAG GAGTGGGGGC GACAGAAGGA GGAGCTCCTG
    TGGCGGGTGG AGCAGTTACA GAAAGAGAAA AGTCCCCGGA GAAGCGGCAG TTTCCTCTGC
    AGCCGAAGGG AAGACGACAC ACGCCCTTAC CCGCACCAAG GAAGCCTTCA CTCCTCCAGA
    CCTGTGTCCA TGTGGCCCTG TGAAGACGCC GACTCCATCC CATTTGAAGA CCGGCCGCTC
    TCCAAACTGA AGGAGTCGGA CAGGTGCTCG GCCAGTGAGA ACCTGTACTT GGACGCTTTG
    TCCCTGGATG ATGATCCAGG GGATCCGCCA CCCCTCAGGA ACTGCCTTGC CGAGGAAGAA
    GAAAGTCGCA AGGGCAATCT TCAGAGGGCT GTGTCTGTGT CCTGTATGTC CGAGTTCCAG
    CGTCTGATGG ACGTCTCTCC GTTCCTGCCT GAGAAGGGGC TACCTTCCGC CGGCAGCAAG
    GAGGATGTCA CTCCACCCTT GTCTCCCGAC GACTTGAAAT ACATTGAAGA ATTCCATAGC
    AACGACTGGG ATTACGCATC CCCCAGGGCT GAGGCCGATC GGCCTCCAGA CCCCTGGGCA
    GACAGGACCG AGATGGGGAG GGTGGGGCAT GAAGCCACCA CAGAGCCTTG TCCGGATCCC
    TCCTGGTATC TGACCACAAG TGTCACGATG ACGACAGACA CCATGACCAG TCCGGAGCAC
    TGCCAGAAGC AGCCACTGCG GACCCATGTC CTCACTGAGC AGTCTGGTGT GCACGTGCTG
    CACAGCCCGC CTGCCATTCG CCGGGTTGAC AGTATAGCAT CAGGCGGCGA GGGCAGGAGC
    CGAGCGGACC CCGAGGGCCC CTTTCCTATG AGCAGAGCAA GAGGGAACCT GGCAGATGCC
    AAGGGGGGTC ATCCAGAACC TGTGCTCAAC AGGTGGCCTT GCACGCCACC CAGACATCCC
    CGGGACTGTG TGGAGGGGTC TCTTCGCCCC CTCGACAGAC CTATCTGCCC CTCCCTGGGA
    TTTGCCTCCC CTCTGAACAG CCTAGATATG TCTAAGAACA TGAGTGATGA CATGAAGGAG
    GTGGCTTTTT CTGTCAGGAA TGCCATCTGC GCCGGTCCTG CCGAGCCACA CGTCAGGGAT
    ATGGCCTGCC AGACCAATGG GTCCAGGACA GCAGGGACGC AGACCATCCA GACAATCAGC
    GTGGGCCTGC AGACCGAGGC CCTACGTGCC AGCGGCGTCA CCAGCAGCCC CCACAAGTGT
    CTCACGCCAA AAGCCGGAGG TGGCACCACA CCCGTGTCTT CTCCTTCCCG CAGCCTTAGG
    AGTAGACAGG TGGCCCCTGC CATTGAGAAG GTGCAGGCCA AGTTTGAACG CACCTGCTGC
    TCCCCAAAGT ACGGTTCTCC CAAACTTCAG AGAAAGCCCC TCTCAAAAGC TGACCAGCCA
    AATAGCAGGA CCTCACCAGG CATACCCCAG AAAGGGTTCA GCGAGTCAGC CTGGGCCCGC
    TCCACCACCA CGAGGGAGAG CCCTGTGCAC ACCACCATCA ATGATGGCCT CTCCAGCCTC
    TTCAATATCA TCGACCACAG TCCCATCGGG GTGCGGGCTG GGAGCCGATC TCGGTCAGCA
    GAACCTCGAC AGGAGCTGGG TCCAGGCCAG GAAACGGGCA CCAGTTCCAG AGGGAGGTCG
    CCCAGCCCGC TAGGGGTAGG CTCCGAGACG TTCAGGGAGG AGGGAGGAGA GAGCACGCCA
    GTGAGACAGG ACTTATCTGC GCCCCCTGGC TACACGCTCA CTGAGAATGT GGCCCGGATC
    CTCAACAAAA AGTTGTTGGA GCATGCCTTA AAGGAGGAGA GGAAGCAAGC CAGCCATGGC
    TCCTCAGGTC TCACCAGCGA CAGCCACACT GGAGAACCAG TGCCAGCCGA GCCAGGATCC
    ATGGAGAACC AAACTGTCTT ACTAACTGCC CCCTGGGGAC TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244889.1
    CDS462..6266
    Translation

    Target ORF information:

    RefSeq Version XM_011246587.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246587.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGGATGACA GTGCGGATTT GAAGTGTCAG
    CTTCAGTTTG TCAAAGAGGA AGCTTCCCTG ATGCGCAAAA AGATGGCCAA GTTGGGGCGG
    GAGAAGGACG AGTTGGAGCA GGAACTGCAG AAGTATAAGT CCCTCTATGG TGATGTGGAC
    AGTCCCCTCC CAACAGGGGA GGCTGGTGGT CCCCCCAGCA CCAGGGAGGC AGAGCTGAAA
    CTGCGGCTGA AGTTGGTGGA GGAGGAAGCC AGCATCTTGG GCAGGAAGAT CGTGGAGCTG
    GAGGTGGAAA ACCGTGGCCT CAAAGCAGAG ATGGAAGACA TACGGGTTCA GCATGAACGC
    GAGGGAACAG GCAGGGACCA CGTGCCAAGC ACTCCTACCT CACCCTTCGG AGACTCAATG
    GAGTCCTCCA CGGAGCTCCG CCGGCACCTG CAGTTTGTGG AAGAAGAGGC TGAGCTGCTG
    AGGAGATCCA TCTCTGAGAT TGAAGATCAC AACCGGCAGT TGACCCACGA GCTGAGCAAG
    TTCAAGTTTG AGCCTCACCA GGAGTCAGGG TGGCTTGGGG ACGGTGTGTC TAAGGGCCCC
    GCGGCCAGCG TTCCCCTGCA GGAGGAGCTG AAGTCAGCCA GGCTACAGAT CGATGAGCTG
    AGCGGGAAGG TACTGAAGCT GCAGTGTGAG AACCGGCTGC TTCTGTCCAA TGCCCAACGG
    GGTGACCTCG CCGCACATCT GGGGCTGCGC GCTCCTAGCC CCCGGGACAG TGATGCCGAG
    AGTGACGCGG GCAAGAAAGA GAGCGATGGG GAGGAGGGCC GCCTTCCCCA GCCAAAGCGA
    GAAGGCCCTG TTGGTGGGGA GAGCGACTCG GAGGACATGT TTGAGAAGAC CTCGGGCTTC
    GGAAGCGGAA AGCCGTCGGA GGCTAGTGAG CCATGCCCGG CAGAGCTGCT AAGGGTCCGG
    GAGGATACCG AGTGCCTGGT GACCATAAAG CTGGAGGCTC AGCGGCTTGA GCGGACTGTG
    GAGCGCCTCA TCTCTGATAC AGATGGTTTC ATCCACGACT CAGGACTGCG GGGCAATGGA
    TTGGCCTCAC CTGGAGTCCA AGGCGGAGGA GGTGAGGGCA ACAGCCCGAG TGAGCCCCAC
    TTGTTGGAGA CCATCAATGT GAGGATGAAG GCTTTCAGGA AAGAGCTGCA GGCCTTCCTG
    GAGCAGATGT CCCGGATTGT GGATGGTCTC TCGCCCTTAT CCCACCTCAC AGAGTCATCC
    AGCTTCCTCT CCACTGTGAC TTCTGTGTCC CGGGACTCCC CCATCGGGAC CCTGGGAAAG
    GAGTTGGGCC CAGACTTGCA GTCCAAACTG AGAGAGCAGC TGGAATGGCA GCTCAATCAG
    GATCGAGGGG ACGAGCGAGA GGGCCTGCGT CTCCGAGCCA CTCGGGAGCT GCACCGGCGC
    GCGGATGGGG ATTCCGGGAG CCACCATGGG CTGGGAGGCC AGAGCTGTTT CAACCTAGAG
    CTCAGGGGCT CCCCAGTCTT ACCTGAGCAG AGTGTGTCTG TAGAAGAGCT ACAGGGTCAG
    CTCGAGCAGG CGGCCAGACT CCATCAAGAG GAGACAGAGA CATACACAAA CAAGATCCGT
    AAGATGGAGG AGGACCACCT CTACGCCCTG CGGTGGAAGG AACTGGAGAT GCACAGCCTG
    GCCCTGCAGA ACACCCTTCA CAAGCGGACG TGGAGCGACG AGAAGAATCT GCTGCAGCAG
    GAGCTCAGGT CCCTGAAGCA GAACATCTTC CTCTTCTACG TCAAGCTCAG GTGGCTGCTG
    AAACACTGGC GCCAAGGGAA GCAGATGGAG GAGGGAGGAG AGGATCTGGA GGAGAGCGAA
    CATCCAGAGA ATGTGCCTGG GCTTGCTGAG CTTGGAGTCC AGGGGGTTCA CCAGACAGAT
    GGGATAGACC AGGAGGATGC TGACCAAGGC TGTAGCCTTC CCATGGGAGA GCATGCCCCA
    CACTCCCTGG TACAGATCAG CGAACACGGA TCACGGCTAC AGAGTTCAGA CGGGGGACCA
    CTTAACAAAC AGGTGGTGGA AAACCAGCAG CTCTTCAGGG CCCTCAAGGC TCTGCTGGAG
    GACTTCCGTT CGGAGCTGCG CGAAGACGAG CATGCGCGGC TGCGGCTGCA GCAGCAGTAC
    GCCAGCGACA AGGCTGCGTG GGATGTGGAG TGGGCTGTGC TCAAGTGCCG CCTGGAACAG
    CTGGAAGAGA AGACTGAGAA AAGCTTGGGC GAGCTGGACT CCTCTGCTGA GGGCAAAGGG
    GCCTTGAAGA AGGAGAGAGA GGTGCACCAG AAACTGCTGG CTGACAGCCA CAGCCTGGTG
    ATGGACCTGC GCTGGCAGAT CCATCACCGA GAGAAGAACT GGAACCGGGA GAAGGTGGAG
    CTGCTGGAGC GTCTGGACAG CGAGCGGCAG GAGTGGGGGC GACAGAAGGA GGAGCTCCTG
    TGGCGGGTGG AGCAGTTACA GAAAGAGAAA AGTCCCCGGA GAAGCGGCAG TTTCCTCTGC
    AGCCGAAGGG AAGACGACAC ACGCCCTTAC CCGCACCAAG GAAGCCTTCA CTCCTCCAGA
    CCTGTGTCCA TGTGGCCCTG TGAAGACGCC GACTCCATCC CATTTGAAGA CCGGCCGCTC
    TCCAAACTGA AGGAGTCGGA CAGGTGCTCG GCCAGTGAGA ACCTGTACTT GGACGCTTTG
    TCCCTGGATG ATGATCCAGG GGATCCGCCA CCCCTCAGGA ACTGCCTTGC CGAGGAAGAA
    GAAAGTCGCA AGGGCAATCT TCAGAGGGCT GTGTCTGTGT CCTGTATGTC CGAGTTCCAG
    CGTCTGATGG ACGTCTCTCC GTTCCTGCCT GAGAAGGGGC TACCTTCCGC CGGCAGCAAG
    GAGGATGTCA CTCCACCCTT GTCTCCCGAC GACTTGAAAT ACATTGAAGA ATTCCATAGC
    AACGACTGGG ATTACGCATC CCCCAGGGCT GAGGCCGATC GGCCTCCAGA CCCCTGGGCA
    GACAGGACCG AGATGGGGAG GGTGGGGCAT GAAGCCACCA CAGAGCCTTG TCCGGATCCC
    TCCTGGTATC TGACCACAAG TGTCACGATG ACGACAGACA CCATGACCAG TCCGGAGCAC
    TGCCAGAAGC AGCCACTGCG GACCCATGTC CTCACTGAGC AGTCTGGTGT GCACGTGCTG
    CACAGCCCGC CTGCCATTCG CCGGGTTGAC AGTATAGCAT CAGGCGGCGA GGGCAGGAGC
    CGAGCGGACC CCGAGGGCCC CTTTCCTATG AGCAGAGCAA GAGGGAACCT GGCAGATGCC
    AAGGGGGGTC ATCCAGAACC TGTGCTCAAC AGGTGGCCTT GCACGCCACC CAGACATCCC
    CGGGACTGTG TGGAGGGGTC TCTTCGCCCC CTCGACAGAC CTATCTGCCC CTCCCTGGGA
    TTTGCCTCCC CTCTGAACAG CCTAGATATG TCTAAGAACA TGAGTGATGA CATGAAGGAG
    GTGGCTTTTT CTGTCAGGAA TGCCATCTGC GCCGGTCCTG CCGAGCCACA CGTCAGGGAT
    ATGGCCTGCC AGACCAATGG GTCCAGGACA GCAGGGACGC AGACCATCCA GACAATCAGC
    GTGGGCCTGC AGACCGAGGC CCTACGTGCC AGCGGCGTCA CCAGCAGCCC CCACAAGTGT
    CTCACGCCAA AAGCCGGAGG TGGCACCACA CCCGTGTCTT CTCCTTCCCG CAGCCTTAGG
    AGTAGACAGG TGGCCCCTGC CATTGAGAAG GTGCAGGCCA AGTTTGAACG CACCTGCTGC
    TCCCCAAAGT ACGGTTCTCC CAAACTTCAG AGAAAGCCCC TCTCAAAAGC TGACCAGCCA
    AATAGCAGGA CCTCACCAGG CATACCCCAG AAAGGGTTCA GCGAGTCAGC CTGGGCCCGC
    TCCACCACCA CGAGGGAGAG CCCTGTGCAC ACCACCATCA ATGATGGCCT CTCCAGCCTC
    TTCAATATCA TCGACCACAG TCCCATCGGG GTGCGGGCTG GGAGCCGATC TCGGTCAGCA
    GAACCTCGAC AGGAGCTGGG TCCAGGCCAG GAAACGGGCA CCAGTTCCAG AGGGAGGTCG
    CCCAGCCCGC TAGGGGTAGG CTCCGAGACG TTCAGGGAGG AGGGAGGAGA GAGCACGCCA
    GTGAGACAGG ACTTATCTGC GCCCCCTGGC TACACGCTCA CTGAGAATGT GGCCCGGATC
    CTCAACAAAA AGTTGTTGGA GCATGCCTTA AAGGAGGAGA GGAAGCAAGC CAGCCATGGC
    TCCTCAGGTC TCACCAGCGA CAGCCACACT GGAGAACCAG TGCCAGCCGA GCCAGGATCC
    ATGGAGAACC AAACTGTCTT ACTAACTGCC CCCTGGGGAC TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79656
    Clone ID Related Accession (Same CDS sequence) XM_017317622.1 , XM_017317622.2
    Accession Version XM_017317622.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product microtubule cross-linking factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGAT GGAGGAGGAC
    CACCTCTACG CCCTGCGGTG GAAGGAACTG GAGATGCACA GCCTGGCCCT GCAGAACACC
    CTTCACAAGC GGACGTGGAG CGACGAGAAG AATCTGCTGC AGCAGGAGCT CAGGTCCCTG
    AAGCAGAACA TCTTCCTCTT CTACGTCAAG CTCAGGTGGC TGCTGAAACA CTGGCGCCAA
    GGGAAGCAGA TGGAGGAGGG AGGAGAGGAT CTGGAGGAGA GCGAACATCC AGAGAATGTG
    CCTGGGCTTG CTGAGCTTGG AGTCCAGGGG GTTCACCAGA CAGATGGGAT AGACCAGGAG
    GATGCTGACC AAGGCTGTAG CCTTCCCATG GGAGAGCATG CCCCACACTC CCTGGTACAG
    ATCAGCGAAC ACGGATCACG GCTACAGAGT TCAGACGGGG GACCACTTAA CAAACAGGTG
    GTGGAAAACC AGCAGCTCTT CAGGGCCCTC AAGGCTCTGC TGGAGGACTT CCGTTCGGAG
    CTGCGCGAAG ACGAGCATGC GCGGCTGCGG CTGCAGCAGC AGTACGCCAG CGACAAGGCT
    GCGTGGGATG TGGAGTGGGC TGTGCTCAAG TGCCGCCTGG AACAGCTGGA AGAGAAGACT
    GAGAAAAGCT TGGGCGAGCT GGACTCCTCT GCTGAGGGCA AAGGGGCCTT GAAGAAGGAG
    AGAGAGGTGC ACCAGAAACT GCTGGCTGAC AGCCACAGCC TGGTGATGGA CCTGCGCTGG
    CAGATCCATC ACCGAGAGAA GAACTGGAAC CGGGAGAAGG TGGAGCTGCT GGAGCGTCTG
    GACAGCGAGC GGCAGGAGTG GGGGCGACAG AAGGAGGAGC TCCTGTGGCG GGTGGAGCAG
    TTACAGAAAG AGAAAAGTCC CCGGAGAAGC GGCAGTTTCC TCTGCAGCCG AAGGGAAGAC
    GACACACGCC CTTACCCGCA CCAAGGAAGC CTTCACTCCT CCAGACCTGT GTCCATGTGG
    CCCTGTGAAG ACGCCGACTC CATCCCATTT GAAGACCGGC CGCTCTCCAA ACTGAAGGAG
    TCGGACAGGT GCTCGGCCAG TGAGAACCTG TACTTGGACG CTTTGTCCCT GGATGATGAT
    CCAGGGGATC CGCCACCCCT CAGGAACTGC CTTGCCGAGG AAGAAGAAAG TCGCAAGGGC
    AATCTTCAGA GGGCTGTGTC TGTGTCCTGT ATGTCCGAGT TCCAGCGTCT GATGGACGTC
    TCTCCGTTCC TGCCTGAGAA GGGGCTACCT TCCGCCGGCA GCAAGGAGGA TGTCACTCCA
    CCCTTGTCTC CCGACGACTT GAAATACATT GAAGAATTCC ATAGCAACGA CTGGGATTAC
    GCATCCCCCA GGGCTGAGGC CGATCGGCCT CCAGACCCCT GGGCAGACAG GACCGAGATG
    GGGAGGGTGG GGCATGAAGC CACCACAGAG CCTTGTCCGG ATCCCTCCTG GTATCTGACC
    ACAAGTGTCA CGATGACGAC AGACACCATG ACCAGTCCGG AGCACTGCCA GAAGCAGCCA
    CTGCGGACCC ATGTCCTCAC TGAGCAGTCT GGTGTGCACG TGCTGCACAG CCCGCCTGCC
    ATTCGCCGGG TTGACAGTAT AGCATCAGGC GGCGAGGGCA GGAGCCGAGC GGACCCCGAG
    GGCCCCTTTC CTATGAGCAG AGCAAGAGGG AACCTGGCAG ATGCCAAGGG GGGTCATCCA
    GAACCTGTGC TCAACAGGTG GCCTTGCACG CCACCCAGAC ATCCCCGGGA CTGTGTGGAG
    GGGTCTCTTC GCCCCCTCGA CAGACCTATC TGCCCCTCCC TGGGATTTGC CTCCCCTCTG
    AACAGCCTAG ATATGTCTAA GAACATGAGT GATGACATGA AGGAGGTGGC TTTTTCTGTC
    AGGAATGCCA TCTGCGCCGG TCCTGCCGAG CCACACGTCA GGGATATGGC CTGCCAGACC
    AATGGGTCCA GGACAGCAGG GACGCAGACC ATCCAGACAA TCAGCGTGGG CCTGCAGACC
    GAGGCCCTAC GTGCCAGCGG CGTCACCAGC AGCCCCCACA AGTGTCTCAC GCCAAAAGCC
    GGAGGTGGCA CCACACCCGT GTCTTCTCCT TCCCGCAGCC TTAGGAGTAG ACAGGTGGCC
    CCTGCCATTG AGAAGGTGCA GGCCAAGTTT GAACGCACCT GCTGCTCCCC AAAGTACGGT
    TCTCCCAAAC TTCAGAGAAA GCCCCTCTCA AAAGCTGACC AGCCAAATAG CAGGACCTCA
    CCAGGCATAC CCCAGAAAGG GTTCAGCGAG TCAGCCTGGG CCCGCTCCAC CACCACGAGG
    GAGAGCCCTG TGCACACCAC CATCAATGAT GGCCTCTCCA GCCTCTTCAA TATCATCGAC
    CACAGTCCCA TCGGGGTGCG GGCTGGGAGC CGATCTCGGT CAGCAGAACC TCGACAGGAG
    CTGGGTCCAG GCCAGGAAAC GGGCACCAGT TCCAGAGGGA GGTCGCCCAG CCCGCTAGGG
    GTAGGCTCCG AGACGTTCAG GGAGGAGGGA GGAGAGAGCA CGCCAGTGAG ACAGGACTTA
    TCTGCGCCCC CTGGCTACAC GCTCACTGAG AATGTGGCCC GGATCCTCAA CAAAAAGTTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAAG CAAGCCAGCC ATGGCTCCTC AGGTCTCACC
    AGCGACAGCC ACACTGGAGA ACCAGTGCCA GCCGAGCCAG GATCCATGGA GAACCAAACT
    GTCTTACTAA CTGCCCCCTG GGGACTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173111.1
    CDS462..6251
    Translation

    Target ORF information:

    RefSeq Version XM_017317622.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus microtubule crosslinking factor 1 (Mtcl1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCCGG ACACCAAGCC GCAGCCAGCC 
    GGCCAGCATC ACCGCCACCA CCACCTGCAC CCGCTGGCCG AGAGGAGGCG GCTGCACCGC
    GCACCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACA CGCGGCCCGC CACGGCCACC
    CCCTCTGCGG GCCGTGCCCC GACTCCCGCG GCTCCTCGCT CGCCTAGCCT TGCCGGGAAG
    GCGCCGCCGT CGCCCGGGCC CCCGGCCGCG CCCGGTCGCC TCTCCCGACG CAGCGGTGTA
    GTCCCCGGCG CGAAGGACAA GCCGCCGCCG GGCGCCGGAG CCAGGTCGGC GGGCGGCGCT
    AAGGCCGTCC CGGGGACCAG GAGGGCGGCG CGCGCGGGGC CGGCGGAGCC ACTGTCCCGG
    GTTGGGAGGC CGACTGGGGC CGAGCCACCG CCTGCCGTCG CGAAGGGTCG CAAAACCAAG
    CGCGGCCCCG GGACGCCTCC AGCTCGCGCC GTCGTGCCCC CGGCTCGCGC CTCCCGGGTC
    CCCGCCGTGA CCCTCTCTGT GACCTCGGTG GCTGGCTGTC GTATCAACCA CACGGACAGC
    AGCTCGGATC TCTCCGACTG CGCCTCCGAA CCCTTGTCTG ATGAGCAGCG CCTACTCCCC
    GCCGCCAGCA GCGACGCTGA GTCGGGCACC GGCTCCAGTG ACCGGGAACC CATACGCGGA
    GCACCCACTC CGAGCTCCGG ATCCCGCGGG CCGCCGCCGG GCAGCCCCGA GCCCCCTATC
    CTGCTCGCGG CGCCCCCTGT CGCCAGTGCC TGTCTCGGGG GCCGAAGCAG CCCAGGCGGG
    GCCTCCACAG GGTCCCCGGG ACCCGGTTCT CAGGAGGATG TTGGGGGCCG AGCGCCACCG
    GAGCGGACGA TTCTGGGGAC CTCCAAGGAG CCTAGCCTGG GCGAACAGCC CCGGCTCTTG
    GTGGTGGCTG AGGAAGAGGA GTTGCTGAGG GAGATGGAGG AACTGCGCTC GGAGAACGAC
    TACCTCAAGG ACGAGCTGGA TGAGCTCCGT GCAGAGATGG AGGAGATGAG GGATAGCTAT
    CTGGAGGAGG ACGGGTACCA GCTGCAGGAG CTCCGGAGAG AGCTGGACCG TGCTAACAAG
    AACTGCAGAA TCCTGCAGTA CCGACTCAGG AAAGCCGAGC AGAAGAGCCT GAAAGTGGCG
    GAAACAGGGC AGGTGGACGG GGAGCTCATC AGAAGCCTGG AGCAGGACTT GAAGGTAGCC
    AAAGATGTGT CTGTCAGATT ACACCACGAA CTCGAGACCG TGGAGGAGAA GCGCGCTAAG
    GCTGAAGACG ACAACGAAAC GCTCCGGCAG CAGATGATCG AGGTGGAGGT ATCCAGACAG
    GCCCTCCAGA ATGAGGTGGA GAGACTGAGG GAGAGTTCTC TGAAGAGAAG AGGCAGTCGG
    GAAATGTACA AAGAGAAGAA ACTTGTTAAC CAGAATGGTG GAGTGGAACG TCCTGGGAAC
    TGCAGGTCGG CCACCAAAAC CCAAAGGAAG CTGGCACCGA GGCGCAAGAT AACCCTCCCT
    CGGGCTATTC TTGCCTGGCA GGATGACAGT GCGGATTTGA AGTGTCAGCT TCAGTTTGTC
    AAAGAGGAAG CTTCCCTGAT GCGCAAAAAG ATGGCCAAGT TGGGGCGGGA GAAGGACGAG
    TTGGAGCAGG AACTGCAGAA GTATAAGTCC CTCTATGGTG ATGTGGACAG TCCCCTCCCA
    ACAGGGGAGG CTGGTGGTCC CCCCAGCACC AGGGAGGCAG AGCTGAAACT GCGGCTGAAG
    TTGGTGGAGG AGGAAGCCAG CATCTTGGGC AGGAAGATCG TGGAGCTGGA GGTGGAAAAC
    CGTGGCCTCA AAGCAGAGAT GGAAGACATA CGGGTTCAGC ATGAACGCGA GGGAACAGGC
    AGGGACCACG TGCCAAGCAC TCCTACCTCA CCCTTCGGAG ACTCAATGGA GTCCTCCACG
    GAGCTCCGCC GGCACCTGCA GTTTGTGGAA GAAGAGGCTG AGCTGCTGAG GAGATCCATC
    TCTGAGATTG AAGATCACAA CCGGCAGTTG ACCCACGAGC TGAGCAAGTT CAAGTTTGAG
    CCTCACCAGG AGTCAGGGTG GCTTGGGGAC GGTGTGTCTA AGGGCCCCGC GGCCAGCGTT
    CCCCTGCAGG AGGAGCTGAA GTCAGCCAGG CTACAGATCG ATGAGCTGAG CGGGAAGGTA
    CTGAAGCTGC AGTGTGAGAA CCGGCTGCTT CTGTCCAATG CCCAACGGGG TGACCTCGCC
    GCACATCTGG GGCTGCGCGC TCCTAGCCCC CGGGACAGTG ATGCCGAGAG TGACGCGGGC
    AAGAAAGAGA GCGATGGGGA GGAGGGCCGC CTTCCCCAGC CAAAGCGAGA AGGCCCTGTT
    GGTGGGGAGA GCGACTCGGA GGACATGTTT GAGAAGACCT CGGGCTTCGG AAGCGGAAAG
    CCGTCGGAGG CTAGTGAGCC ATGCCCGGCA GAGCTGCTAA GGGTCCGGGA GGATACCGAG
    TGCCTGGTGA CCATAAAGCT GGAGGCTCAG CGGCTTGAGC GGACTGTGGA GCGCCTCATC
    TCTGATACAG ATGGTTTCAT CCACGACTCA GGACTGCGGG GCAATGGATT GGCCTCACCT
    GGAGTCCAAG GCGGAGGAGG TGAGGGCAAC AGCCCGAGTG AGCCCCACTT GTTGGAGACC
    ATCAATGTGA GGATGAAGGC TTTCAGGAAA GAGCTGCAGG CCTTCCTGGA GCAGATGTCC
    CGGATTGTGG ATGGTCTCTC GCCCTTATCC CACCTCACAG AGTCATCCAG CTTCCTCTCC
    ACTGTGACTT CTGTGTCCCG GGACTCCCCC ATCGGGACCC TGGGAAAGGA GTTGGGCCCA
    GACTTGCAGT CCAAACTGAG AGAGCAGCTG GAATGGCAGC TCAATCAGGA TCGAGGGGAC
    GAGCGAGAGG GCCTGCGTCT CCGAGCCACT CGGGAGCTGC ACCGGCGCGC GGATGGGGAT
    TCCGGGAGCC ACCATGGGCT GGGAGGCCAG AGCTGTTTCA ACCTAGAGAT GGAGGAGGAC
    CACCTCTACG CCCTGCGGTG GAAGGAACTG GAGATGCACA GCCTGGCCCT GCAGAACACC
    CTTCACAAGC GGACGTGGAG CGACGAGAAG AATCTGCTGC AGCAGGAGCT CAGGTCCCTG
    AAGCAGAACA TCTTCCTCTT CTACGTCAAG CTCAGGTGGC TGCTGAAACA CTGGCGCCAA
    GGGAAGCAGA TGGAGGAGGG AGGAGAGGAT CTGGAGGAGA GCGAACATCC AGAGAATGTG
    CCTGGGCTTG CTGAGCTTGG AGTCCAGGGG GTTCACCAGA CAGATGGGAT AGACCAGGAG
    GATGCTGACC AAGGCTGTAG CCTTCCCATG GGAGAGCATG CCCCACACTC CCTGGTACAG
    ATCAGCGAAC ACGGATCACG GCTACAGAGT TCAGACGGGG GACCACTTAA CAAACAGGTG
    GTGGAAAACC AGCAGCTCTT CAGGGCCCTC AAGGCTCTGC TGGAGGACTT CCGTTCGGAG
    CTGCGCGAAG ACGAGCATGC GCGGCTGCGG CTGCAGCAGC AGTACGCCAG CGACAAGGCT
    GCGTGGGATG TGGAGTGGGC TGTGCTCAAG TGCCGCCTGG AACAGCTGGA AGAGAAGACT
    GAGAAAAGCT TGGGCGAGCT GGACTCCTCT GCTGAGGGCA AAGGGGCCTT GAAGAAGGAG
    AGAGAGGTGC ACCAGAAACT GCTGGCTGAC AGCCACAGCC TGGTGATGGA CCTGCGCTGG
    CAGATCCATC ACCGAGAGAA GAACTGGAAC CGGGAGAAGG TGGAGCTGCT GGAGCGTCTG
    GACAGCGAGC GGCAGGAGTG GGGGCGACAG AAGGAGGAGC TCCTGTGGCG GGTGGAGCAG
    TTACAGAAAG AGAAAAGTCC CCGGAGAAGC GGCAGTTTCC TCTGCAGCCG AAGGGAAGAC
    GACACACGCC CTTACCCGCA CCAAGGAAGC CTTCACTCCT CCAGACCTGT GTCCATGTGG
    CCCTGTGAAG ACGCCGACTC CATCCCATTT GAAGACCGGC CGCTCTCCAA ACTGAAGGAG
    TCGGACAGGT GCTCGGCCAG TGAGAACCTG TACTTGGACG CTTTGTCCCT GGATGATGAT
    CCAGGGGATC CGCCACCCCT CAGGAACTGC CTTGCCGAGG AAGAAGAAAG TCGCAAGGGC
    AATCTTCAGA GGGCTGTGTC TGTGTCCTGT ATGTCCGAGT TCCAGCGTCT GATGGACGTC
    TCTCCGTTCC TGCCTGAGAA GGGGCTACCT TCCGCCGGCA GCAAGGAGGA TGTCACTCCA
    CCCTTGTCTC CCGACGACTT GAAATACATT GAAGAATTCC ATAGCAACGA CTGGGATTAC
    GCATCCCCCA GGGCTGAGGC CGATCGGCCT CCAGACCCCT GGGCAGACAG GACCGAGATG
    GGGAGGGTGG GGCATGAAGC CACCACAGAG CCTTGTCCGG ATCCCTCCTG GTATCTGACC
    ACAAGTGTCA CGATGACGAC AGACACCATG ACCAGTCCGG AGCACTGCCA GAAGCAGCCA
    CTGCGGACCC ATGTCCTCAC TGAGCAGTCT GGTGTGCACG TGCTGCACAG CCCGCCTGCC
    ATTCGCCGGG TTGACAGTAT AGCATCAGGC GGCGAGGGCA GGAGCCGAGC GGACCCCGAG
    GGCCCCTTTC CTATGAGCAG AGCAAGAGGG AACCTGGCAG ATGCCAAGGG GGGTCATCCA
    GAACCTGTGC TCAACAGGTG GCCTTGCACG CCACCCAGAC ATCCCCGGGA CTGTGTGGAG
    GGGTCTCTTC GCCCCCTCGA CAGACCTATC TGCCCCTCCC TGGGATTTGC CTCCCCTCTG
    AACAGCCTAG ATATGTCTAA GAACATGAGT GATGACATGA AGGAGGTGGC TTTTTCTGTC
    AGGAATGCCA TCTGCGCCGG TCCTGCCGAG CCACACGTCA GGGATATGGC CTGCCAGACC
    AATGGGTCCA GGACAGCAGG GACGCAGACC ATCCAGACAA TCAGCGTGGG CCTGCAGACC
    GAGGCCCTAC GTGCCAGCGG CGTCACCAGC AGCCCCCACA AGTGTCTCAC GCCAAAAGCC
    GGAGGTGGCA CCACACCCGT GTCTTCTCCT TCCCGCAGCC TTAGGAGTAG ACAGGTGGCC
    CCTGCCATTG AGAAGGTGCA GGCCAAGTTT GAACGCACCT GCTGCTCCCC AAAGTACGGT
    TCTCCCAAAC TTCAGAGAAA GCCCCTCTCA AAAGCTGACC AGCCAAATAG CAGGACCTCA
    CCAGGCATAC CCCAGAAAGG GTTCAGCGAG TCAGCCTGGG CCCGCTCCAC CACCACGAGG
    GAGAGCCCTG TGCACACCAC CATCAATGAT GGCCTCTCCA GCCTCTTCAA TATCATCGAC
    CACAGTCCCA TCGGGGTGCG GGCTGGGAGC CGATCTCGGT CAGCAGAACC TCGACAGGAG
    CTGGGTCCAG GCCAGGAAAC GGGCACCAGT TCCAGAGGGA GGTCGCCCAG CCCGCTAGGG
    GTAGGCTCCG AGACGTTCAG GGAGGAGGGA GGAGAGAGCA CGCCAGTGAG ACAGGACTTA
    TCTGCGCCCC CTGGCTACAC GCTCACTGAG AATGTGGCCC GGATCCTCAA CAAAAAGTTG
    TTGGAGCATG CCTTAAAGGA GGAGAGGAAG CAAGCCAGCC ATGGCTCCTC AGGTCTCACC
    AGCGACAGCC ACACTGGAGA ACCAGTGCCA GCCGAGCCAG GATCCATGGA GAACCAAACT
    GTCTTACTAA CTGCCCCCTG GGGACTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.