Mto1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mto1
Entrez Gene ID	68291
Full Name	mitochondrial tRNA translation optimization 1
Synonyms	2310039H01Rik,5730419A02Rik
General protein information	Preferred Names mitochondrial tRNA translation optimization 1 Names protein MTO1 homolog, mitochondrial mitochondrial translation optimization 1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026658.2	NP_080934.2	protein MTO1 homolog, mitochondrial precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MTO1	XP_532202.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007661	XP_308209.4
Magnaporthe oryzae (rice blast fungus)	MGG_14754	XP_003713873.1
Danio rerio (zebrafish)	mto1	NP_001076478.1
Caenorhabditis elegans (roundworm)	F52H3.2	NP_496169.1
Eremothecium gossypii	AGOS_AFR255W	NP_985802.2
Oryza sativa (rice)	Os01g0960300	NP_001045465.1
Homo sapiens (human)	MTO1	NP_001116698.1
Pan troglodytes (chimpanzee)	MTO1	XP_003311451.1
Saccharomyces cerevisiae (baker's yeast)	MTO1	NP_011278.2
Kluyveromyces lactis	KLLA0A00935g	XP_451040.1
Schizosaccharomyces pombe (fission yeast)	SPBC30B4.06c	NP_595531.1
Neurospora crassa	NCU11170	XP_956189.2
Bos taurus (cattle)	MTO1	NP_001069537.1
Mus musculus (house mouse)	Mto1	NP_080934.2
Rattus norvegicus (Norway rat)	Mto1	NP_001100311.1
Gallus gallus (chicken)	MTO1	NP_001073232.1
Drosophila melanogaster (fruit fly)	CG4610	NP_611677.1
Arabidopsis thaliana (thale cress)	AT2G13440	NP_178974.1

Gene Ontology
Bibliography

Related articles in PubMed

Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease.
Fakruddin M, Wei FY, Suzuki T, Asano K, Kaieda T, Omori A, Izumi R, Fujimura A, Kaitsuka T, Miyata K, Araki K, Oike Y, Scorrano L, Suzuki T, Tomizawa K
Cell reports22(2)482-496(2018 Jan)

MTO1 mediates tissue specificity of OXPHOS defects via tRNA modification and translation optimization, which can be bypassed by dietary intervention.
Tischner C, Hofer A, Wulff V, Stepek J, Dumitru I, Becker L, Haack T, Kremer L, Datta AN, Sperl W, Floss T, Wurst W, Chrzanowska-Lightowlers Z, De Angelis MH, Klopstock T, Prokisch H, Wenz T
Human molecular genetics24(8)2247-66(2015 Apr)

MTO1-deficient mouse model mirrors the human phenotype showing complex I defect and cardiomyopathy.
Becker L, Kling E, Schiller E, Zeh R, Schrewe A, H?lter SM, Mossbrugger I, Calzada-Wack J, Strecker V, Wittig I, Dumitru I, Wenz T, Bender A, Aichler M, Janik D, Neff F, Walch A, Quintanilla-Fend L, Floss T, Bekeredjian R, Gailus-Durner V, Fuchs H, Wurst W, Meitinger T, Prokisch H, de Angelis MH, Klopstock T
PloS one9(12)e114918(2014)

Identification and characterization of mouse MTO1 gene related to mitochondrial tRNA modification.
Li R, Li X, Yan Q, Qin Mo J, Guan MX
Biochimica et biophysica acta1629(1-3)53-9(2003 Oct)

Isolation and characterization of the putative nuclear modifier gene MTO1 involved in the pathogenesis of deafness-associated mitochondrial 12 S rRNA A1555G mutation.
Li X, Li R, Lin X, Guan MX
The Journal of biological chemistry277(30)27256-64(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MTO1 mediates tRNA modification and controls mitochondrial translation rate in a highly tissue-specific manner associated with tissue-specific oxidative phosphorylation defects.
Title: MTO1 mediates tissue specificity of OXPHOS defects via tRNA modification and translation optimization, which can be bypassed by dietary intervention.

here described is an MTO1-deficient mouse model generated by gene trap mutagenesis that mirrors the human phenotype showing complex I defect and cardiomyopathy.
Title: MTO1-deficient mouse model mirrors the human phenotype showing complex I defect and cardiomyopathy.

These observations suggest that the mouse MTO1 is a structural and functional homolog of yeast MTO1, thereby playing a role in the mitochondrial tRNA modification and protein synthesis.
Title: Identification and characterization of mouse MTO1 gene related to mitochondrial tRNA modification.

The following Mto1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mto1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13218	NM_026658.2 Latest version!	Mus musculus mitochondrial tRNA translation optimization 1 (Mto1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mto1 ( NM_026658.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080934.2 Mus musculus mitochondrial tRNA translation optimization 1 (Mto1), mRNA.

Price & Availability↑

CloneID	OMu13218
Clone ID Related Accession (Same CDS sequence)	NM_026658.2
Accession Version	NM_026658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	protein MTO1 homolog, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY735135.1, CF173757.1, AF369902.1 and BB811598.1. On Mar 29, 2008 this sequence version replaced NM_026658.1. ##Evidence-Data-START## Transcript exon combination :: AK019088.1, SRR1660817.65081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTTC TCCGAGGCCG TGGCCATTGG GCCGCAGCGT CCCTCGGCAG ACGGCTGCCT 
TTGCGTCGCC TCCGCAGCGA CAGCGCGGCG CCCTGCACCC CGCACTTCGA CGTGGTAGTT 
ATCGGAGGAG GGCACGCGGG GACCGAGGCG GCCGCCGCCG CCGCTCGCTG TGGCTCGCGG 
ACCCTGCTTC TCACGCACCG CGTGGACACG ATCGGTCAGA TGTCCTGTAA TCCTTCCTTT 
GGTGGCATCG GAAAGGGGCA TTTGATGAGA GAAGTGGATG CCTTGGATGG TCTGTGTTCC 
CGGATCTGTG ACCAGTCTGG TATACATTAC AAAGTGCTAA ACCGGCGTAA GGGCCCTGCT 
GTGTGGGGAC TGAGAGCACA GATTGACCGG AAACTTTATA AACAGAACAT GCAGAAAGAA 
ATCCTGAGCA CGCCCCTACT CACTGTTCAG AAGGGAGCTG TAGAAGATCT AGTTCTTGCA 
GAACCAGAGC CTGGGTACCC TGGGAAGTCC AGGGTCAGAG GTGTTGTCCT GGCAGATGGA 
AGTACAATTT ATGCAGAGAG TGTAATTCTG ACTACTGGGA CATTTCTGAG AGGCATGATC 
ATAATTGGAT TGGAGATGCA TCCTGCAGGA CGTTTGGGGG ATCAGCCCTC CATAGGGTTG 
GCTCAGACTC TGGAGAAGTT GGGATTTATG GTAGGAAGAC TAAAGACTGG AACTCCACCC 
CGACTCGCTA AAGAGTCCAT TAATTTCTCC ATTCTAAACA AGCACACACC AGATGATCCA 
TCCATACCAT TCAGCTTTCT CAGTGACAGC GTGTGGATCA AGCCGGAAGA CCAACTGCCA 
TGTTACTTGA CTCACACCAA CCCCCGAGTG GATGCGATTG TCCTTGAGAA CCTTCACCTG 
AATAGCCACG TTCAGGAAAC TACAAAAGGG CCCCGATACT GTCCCTCTAT TGAATCAAAG 
GTTTTACGTT TTCCAAATCG TCTGCATCAG GTTTGGTTGG AGCCTGAAGG AATGGATTCC 
GACCTCATCT ACCCGCAAGG GTTATCTGTT ACACTGCCAG CTGAGTTACA GGAGAAAATG 
ATAACATGCA TCAGAGGCTT GGAGAAAGCC AAAATGGTTC ACCCAGGCTA TGGTGTGCAG 
TATGATTATT TAGACCCCCG TCAGATCAGT CCTTCCCTCG AGACACACTT GGTTCAGCGG 
CTGTTCTTTG CTGGACAGAT AAATGGAACC ACTGGCTATG AGGAAGCTGC AGCTCAGGGG 
GTGATAGCTG GAATCAATGC CAGTCTACGA GTCAGTCGAA AGCCACCCTT TGTTGTGAGT 
CGCACAGAAG GCTACATAGG AGTCTTGATT GATGACCTCA CTACCCTGGG CACCAGTGAG 
CCATACCGAA TGTTCACTAG CAGAGTAGAG TTCCGATTGT CACTGCGCCC CGATAATGCT 
GACACCCGGC TCACGTTCCG AGCCCATAAG GAAGCTGGCT GTGTGTCCTC ACAGCGATTT 
GAAAGAGCTT TGTGGATGAA GTCATCTTTA GAAGAAGGTA TTTCTGTGTT GAAATCCATT 
AAATTTTCAA GCTCCAAATG GAAAAAGTTA ATTCCACAAA TTCCTATAAG TATTAATAGA 
AGCCTTCCTG TCAGTGCTCT GGATGTTCTT AAGTATGAAG AAGTGGACAT GGAGTCGCTC 
GTCGGCGTGC TTCCCGAGCC CTTGGAGAAG TATACTGCAT GTAGAGAGCT CGCCAGAAGA 
CTGAAAATAG AAGCCAGTTA TGAGTCAGTA TTGTCATATC AGCTACAAGA AATAAAGGAG 
GTTCAGCAAG ATGAAGCTTT GCAGCTTCCA CACGAGCTCG ACTACCTAAC CATCAGGGAT 
GTGTCATTGT CCCAGGAAGT CCGGGAGAAG CTACACTTGA GTCGCCCACA GACGATTGGG 
GCTGCTAGTC GTATTCCTGG AGTGACACCA GCTGCCATCA TCAATCTGCT CAGATTTGTG 
AGGAGCACGC GGCAGAGCAG ACAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080934.2
CDS	88..2097
Translation	MFLLRGRGHWAAASLGRRLPLRRLRSDSAAPCTPHFDVVVIGGGHAGTEAAAAAARCGSRTLLLTHRVDTIGQMSCNPSFGGIGKGHLMREVDALDGLCSRICDQSGIHYKVLNRRKGPAVWGLRAQIDRKLYKQNMQKEILSTPLLTVQKGAVEDLVLAEPEPGYPGKSRVRGVVLADGSTIYAESVILTTGTFLRGMIIIGLEMHPAGRLGDQPSIGLAQTLEKLGFMVGRLKTGTPPRLAKESINFSILNKHTPDDPSIPFSFLSDSVWIKPEDQLPCYLTHTNPRVDAIVLENLHLNSHVQETTKGPRYCPSIESKVLRFPNRLHQVWLEPEGMDSDLIYPQGLSVTLPAELQEKMITCIRGLEKAKMVHPGYGVQYDYLDPRQISPSLETHLVQRLFFAGQINGTTGYEEAAAQGVIAGINASLRVSRKPPFVVSRTEGYIGVLIDDLTTLGTSEPYRMFTSRVEFRLSLRPDNADTRLTFRAHKEAGCVSSQRFERALWMKSSLEEGISVLKSIKFSSSKWKKLIPQIPISINRSLPVSALDVLKYEEVDMESLVGVLPEPLEKYTACRELARRLKIEASYESVLSYQLQEIKEVQQDEALQLPHELDYLTIRDVSLSQEVREKLHLSRPQTIGAASRIPGVTPAAIINLLRFVRSTRQSRQQ

Target ORF information:

RefSeq Version	NM_026658.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial tRNA translation optimization 1 (Mto1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026658.2

ORF Insert Sequence:

ATGTTCCTTC TCCGAGGCCG TGGCCATTGG GCCGCAGCGT CCCTCGGCAG ACGGCTGCCT 
TTGCGTCGCC TCCGCAGCGA CAGCGCGGCG CCCTGCACCC CGCACTTCGA CGTGGTAGTT 
ATCGGAGGAG GGCACGCGGG GACCGAGGCG GCCGCCGCCG CCGCTCGCTG TGGCTCGCGG 
ACCCTGCTTC TCACGCACCG CGTGGACACG ATCGGTCAGA TGTCCTGTAA TCCTTCCTTT 
GGTGGCATCG GAAAGGGGCA TTTGATGAGA GAAGTGGATG CCTTGGATGG TCTGTGTTCC 
CGGATCTGTG ACCAGTCTGG TATACATTAC AAAGTGCTAA ACCGGCGTAA GGGCCCTGCT 
GTGTGGGGAC TGAGAGCACA GATTGACCGG AAACTTTATA AACAGAACAT GCAGAAAGAA 
ATCCTGAGCA CGCCCCTACT CACTGTTCAG AAGGGAGCTG TAGAAGATCT AGTTCTTGCA 
GAACCAGAGC CTGGGTACCC TGGGAAGTCC AGGGTCAGAG GTGTTGTCCT GGCAGATGGA 
AGTACAATTT ATGCAGAGAG TGTAATTCTG ACTACTGGGA CATTTCTGAG AGGCATGATC 
ATAATTGGAT TGGAGATGCA TCCTGCAGGA CGTTTGGGGG ATCAGCCCTC CATAGGGTTG 
GCTCAGACTC TGGAGAAGTT GGGATTTATG GTAGGAAGAC TAAAGACTGG AACTCCACCC 
CGACTCGCTA AAGAGTCCAT TAATTTCTCC ATTCTAAACA AGCACACACC AGATGATCCA 
TCCATACCAT TCAGCTTTCT CAGTGACAGC GTGTGGATCA AGCCGGAAGA CCAACTGCCA 
TGTTACTTGA CTCACACCAA CCCCCGAGTG GATGCGATTG TCCTTGAGAA CCTTCACCTG 
AATAGCCACG TTCAGGAAAC TACAAAAGGG CCCCGATACT GTCCCTCTAT TGAATCAAAG 
GTTTTACGTT TTCCAAATCG TCTGCATCAG GTTTGGTTGG AGCCTGAAGG AATGGATTCC 
GACCTCATCT ACCCGCAAGG GTTATCTGTT ACACTGCCAG CTGAGTTACA GGAGAAAATG 
ATAACATGCA TCAGAGGCTT GGAGAAAGCC AAAATGGTTC ACCCAGGCTA TGGTGTGCAG 
TATGATTATT TAGACCCCCG TCAGATCAGT CCTTCCCTCG AGACACACTT GGTTCAGCGG 
CTGTTCTTTG CTGGACAGAT AAATGGAACC ACTGGCTATG AGGAAGCTGC AGCTCAGGGG 
GTGATAGCTG GAATCAATGC CAGTCTACGA GTCAGTCGAA AGCCACCCTT TGTTGTGAGT 
CGCACAGAAG GCTACATAGG AGTCTTGATT GATGACCTCA CTACCCTGGG CACCAGTGAG 
CCATACCGAA TGTTCACTAG CAGAGTAGAG TTCCGATTGT CACTGCGCCC CGATAATGCT 
GACACCCGGC TCACGTTCCG AGCCCATAAG GAAGCTGGCT GTGTGTCCTC ACAGCGATTT 
GAAAGAGCTT TGTGGATGAA GTCATCTTTA GAAGAAGGTA TTTCTGTGTT GAAATCCATT 
AAATTTTCAA GCTCCAAATG GAAAAAGTTA ATTCCACAAA TTCCTATAAG TATTAATAGA 
AGCCTTCCTG TCAGTGCTCT GGATGTTCTT AAGTATGAAG AAGTGGACAT GGAGTCGCTC 
GTCGGCGTGC TTCCCGAGCC CTTGGAGAAG TATACTGCAT GTAGAGAGCT CGCCAGAAGA 
CTGAAAATAG AAGCCAGTTA TGAGTCAGTA TTGTCATATC AGCTACAAGA AATAAAGGAG 
GTTCAGCAAG ATGAAGCTTT GCAGCTTCCA CACGAGCTCG ACTACCTAAC CATCAGGGAT 
GTGTCATTGT CCCAGGAAGT CCGGGAGAAG CTACACTTGA GTCGCCCACA GACGATTGGG 
GCTGCTAGTC GTATTCCTGG AGTGACACCA GCTGCCATCA TCAATCTGCT CAGATTTGTG 
AGGAGCACGC GGCAGAGCAG ACAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease.
Cell reports22(2)482-496(2018 Jan)
Fakruddin M,Wei FY,Suzuki T,Asano K,Kaieda T,Omori A,Izumi R,Fujimura A,Kaitsuka T,Miyata K,Araki K,Oike Y,Scorrano L,Suzuki T,Tomizawa K

MTO1 mediates tissue specificity of OXPHOS defects via tRNA modification and translation optimization, which can be bypassed by dietary intervention.
Human molecular genetics24(8)2247-66(2015 Apr)
Tischner C,Hofer A,Wulff V,Stepek J,Dumitru I,Becker L,Haack T,Kremer L,Datta AN,Sperl W,Floss T,Wurst W,Chrzanowska-Lightowlers Z,De Angelis MH,Klopstock T,Prokisch H,Wenz T

MTO1-deficient mouse model mirrors the human phenotype showing complex I defect and cardiomyopathy.
PloS one9(12)e114918(2014)
Becker L,Kling E,Schiller E,Zeh R,Schrewe A,H?lter SM,Mossbrugger I,Calzada-Wack J,Strecker V,Wittig I,Dumitru I,Wenz T,Bender A,Aichler M,Janik D,Neff F,Walch A,Quintanilla-Fend L,Floss T,Bekeredjian R,Gailus-Durner V,Fuchs H,Wurst W,Meitinger T,Prokisch H,de Angelis MH,Klopstock T

Identification and characterization of mouse MTO1 gene related to mitochondrial tRNA modification.
Biochimica et biophysica acta1629(1-3)53-9(2003 Oct)
Li R,Li X,Yan Q,Qin Mo J,Guan MX

Isolation and characterization of the putative nuclear modifier gene MTO1 involved in the pathogenesis of deafness-associated mitochondrial 12 S rRNA A1555G mutation.
The Journal of biological chemistry277(30)27256-64(2002 Jul)
Li X,Li R,Lin X,Guan MX