Dnaaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnaaf1
Entrez Gene ID	68270
Full Name	dynein, axonemal assembly factor 1
Synonyms	4930457P18Rik,Lrrc50,m4Bei
General protein information	Preferred Names dynein, axonemal assembly factor 1 Names dynein assembly factor 1, axonemal leucine rich repeat containing 50 leucine-rich repeat-containing protein 50
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026648.4	NP_080924.1	dynein assembly factor 1, axonemal

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dnaaf1	NP_080924.1
Homo sapiens (human)	DNAAF1	NP_848547.4
Pan troglodytes (chimpanzee)	LRRC50	XP_511137.2
Canis lupus familiaris (dog)	DNAAF1	XP_536764.3
Xenopus tropicalis (tropical clawed frog)	LOC101730543	XP_004913593.1
Rattus norvegicus (Norway rat)	Dnaaf1	NP_001014176.1
Bos taurus (cattle)	DNAAF1	NP_001030422.2

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice.
Ha S, Lindsay AM, Timms AE, Beier DR
G3 (Bethesda, Md.)6(8)2479-87(2016 08)

A forward genetic screen in mice identifies mutants with abnormal cortical patterning.
Ha S, Stottmann RW, Furley AJ, Beier DR
Cerebral cortex (New York, N.Y. : 1991)25(1)167-79(2015 Jan)

Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.
Dong F, Shinohara K, Botilde Y, Nabeshima R, Asai Y, Fukumoto A, Hasegawa T, Matsuo M, Takeda H, Shiratori H, Nakamura T, Hamada H
The Journal of cell biology204(2)203-13(2014 Jan)

Deletions and point mutations of LRRC50 cause primary ciliary dyskinesia due to dynein arm defects.
Loges NT, Olbrich H, Becker-Heck A, H?ffner K, Heer A, Reinhard C, Schmidts M, Kispert A, Zariwala MA, Leigh MW, Knowles MR, Zentgraf H, Seithe H, N?rnberg G, N?rnberg P, Reinhardt R, Omran H
American journal of human genetics85(6)883-9(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in Dnaaf1 cause hydrocephalus, laterality defects, and sinusitis.
Title: Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice.

LRRC50 plays a role in assembly of distinct dynein-arm complexes
Title: Deletions and point mutations of LRRC50 cause primary ciliary dyskinesia due to dynein arm defects.

The following Dnaaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnaaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02749	NM_026648.4 Latest version!	Mus musculus dynein, axonemal assembly factor 1 (Dnaaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnaaf1 ( NM_026648.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080924.1 Mus musculus dynein, axonemal assembly factor 1 (Dnaaf1), mRNA.

Price & Availability↑

CloneID	OMu02749
Clone ID Related Accession (Same CDS sequence)	NM_026648.4
Accession Version	NM_026648.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	dynein assembly factor 1, axonemal
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104882.30. On Oct 19, 2007 this sequence version replaced NM_026648.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK019633.1, BC050751.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACCCCG AAGTCTCTGA GCCGCCGGTG GACAGCGTGG CGGAGCCCAG TCTGGAGGAG 
TCGGCAGGCG ATCACGGCGA CGCGGGGCCA GGGATCCGTA AAGAAGAAAT TAGTGAGACC 
AAGGAAACAT GTGCGGGTCC TTGCACAACA TCCTGCCCAA GTCAGCAGCA ACCAAGTGGC 
GACAACGGGT CAGAGGGCTT CTGCACCCAC TCGAGAGATG ACAGAGAGGA TCGGGGCCCC 
AGGATGACGA AACAGTTCCT TCAAAAGCTC TGCAAGCAGC ACAAGCTGTA CGTCACCCCC 
GCCCTGAACG ACACCCTGTA TTTGCACTTT AAGGGCTTTG ACCGCATTGA GAACCTGGAG 
GAGTACACGG GCCTGCGCTG CCTCTGGCTG GAGTGCAATG GCATCCAGAG GATCGAGAAT 
CTGCAGGCCC AGAGCGAACT GCGCTGCCTC TTCCTGCAAG TGAACCTGTT GCACAAGATC 
GAGAACCTGG AGCCCCTCCA GAAGCTGGAC GCCCTCAACC TGAGCAACAA CTACATCAAG 
ACCATTGAGA ACCTCTCCTG CCTCCCTGTC CTGAACACAC TGCAAATGGC CCACAACCGC 
CTGGAGACGG TGGCTGACAT TGAGCACCTC AGGGAGTGTC TGCGACTCTG TGTCCTCGAC 
CTCTCCCACA ATGCGCTGAG TGACCCTGAG ATCCTGAGCG TGCTAGAGAG CATGCCCTGT 
CTGCGTGTGC TGAACCTCAT GGGAAACCCA GTGACCAAAC ACATCCCTAA CTACCGCAGG 
ACGGTCACTG TCCGTCTGAA GCACTTGACT TATCTGGATG ACAGGCCAGT TTTCCCCAAG 
GACAGGGCGT GTGCAGAGGC ATGGGCCCGA GGTGGGTATG CCGCCGAGAA GGAAGAGCGT 
CGCCAGTGGG AGAGCAGGGA ACACAAGAAG ATAACAGACA GCCTGGAAGC CTTGGCCATG 
ATCAAGCGTC GGGCTGAGGA GCGGAAGAAG GCAAGAGATA GAGGGGAGAC ACCCCTGCCA 
GACAGCGAAG GGAGTATCCC CACCAGCCCG GAGGCTGAGG AAAAGCAACC CATGGGGGAA 
ATCCAGAAGA AGATGGAGTT GTTTGTGGAG GAGAGCTTTG AAGCCAAAGA TGAACTGTTT 
CCAGAGACGC CAGGAGGAGA AAAGGAGCTG CATGTGGTTG TGGTGAATGG GGCAGTGGAG 
AATCCAGACC TGTCTGGGAG CCTGGCTCAC AACCAGACAC CTGTGGTGGT CACCCCGGAA 
GAGTCTACAT CTCCAGTGGC AGCTACTGAT GGGGCCAGAA CTGAAGATAT AGAGGCCGTT 
GCCGTGGAGA TCAAGGAGAG GCTTTTCATT GATGACCTTC CTGACTTGGA AGATGCTGAG 
GGTACAGATG TGTCTGTGGA AGACCAGATA AAGGAGACAG ACATCCCGAG AATCCAGGCC 
ATTTCCAGCT TGAGTGACGA CAGTGACCCG GAGCTGGACG AGCTGTCCCT CTCCACGTCT 
GAGGCCACTC CCACAGGCGC CACAGGTGCC CTCTCACACA TATTTGCCAT CTCCAAGGGC 
CCCTCCACGG CAGCCACGGT GCCTTTCACA GACATATGTA AGCCAATAGC AACAACGGAC 
TTAGAAAGCC AAGGTCAAGA CTGCGGTGCA GCGGCCTCAC GCCCCCTCAT CCAGGAGCTC 
AATGATGAGC CGGCAGAGGA AGCAGCAAAC CAGCCACTGC CGCCCCAAAC CTGTGCGAGT 
GACCCTGCAC TTGCCCACCC TAGTGAAGAT GGAGACAGCG ACAGCCAGCT GCCTGCAGCC 
ACTCTGCTAG GAGATGGAGC GGAGGATGAA GCAGAGTCTT CGGTGCACCC GAAGGAGCCC 
AGCACCCGGG TAGGCCTAGA AGATATTGAA TTTGGCTTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080924.1
CDS	128..2032
Translation	MHPEVSEPPVDSVAEPSLEESAGDHGDAGPGIRKEEISETKETCAGPCTTSCPSQQQPSGDNGSEGFCTHSRDDREDRGPRMTKQFLQKLCKQHKLYVTPALNDTLYLHFKGFDRIENLEEYTGLRCLWLECNGIQRIENLQAQSELRCLFLQVNLLHKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNRLETVADIEHLRECLRLCVLDLSHNALSDPEILSVLESMPCLRVLNLMGNPVTKHIPNYRRTVTVRLKHLTYLDDRPVFPKDRACAEAWARGGYAAEKEERRQWESREHKKITDSLEALAMIKRRAEERKKARDRGETPLPDSEGSIPTSPEAEEKQPMGEIQKKMELFVEESFEAKDELFPETPGGEKELHVVVVNGAVENPDLSGSLAHNQTPVVVTPEESTSPVAATDGARTEDIEAVAVEIKERLFIDDLPDLEDAEGTDVSVEDQIKETDIPRIQAISSLSDDSDPELDELSLSTSEATPTGATGALSHIFAISKGPSTAATVPFTDICKPIATTDLESQGQDCGAAASRPLIQELNDEPAEEAANQPLPPQTCASDPALAHPSEDGDSDSQLPAATLLGDGAEDEAESSVHPKEPSTRVGLEDIEFGLD

Target ORF information:

RefSeq Version	NM_026648.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein, axonemal assembly factor 1 (Dnaaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026648.4

ORF Insert Sequence:

ATGCACCCCG AAGTCTCTGA GCCGCCGGTG GACAGCGTGG CGGAGCCCAG TCTGGAGGAG 
TCGGCAGGCG ATCACGGCGA CGCGGGGCCA GGGATCCGTA AAGAAGAAAT TAGTGAGACC 
AAGGAAACAT GTGCGGGTCC TTGCACAACA TCCTGCCCAA GTCAGCAGCA ACCAAGTGGC 
GACAACGGGT CAGAGGGCTT CTGCACCCAC TCGAGAGATG ACAGAGAGGA TCGGGGCCCC 
AGGATGACGA AACAGTTCCT TCAAAAGCTC TGCAAGCAGC ACAAGCTGTA CGTCACCCCC 
GCCCTGAACG ACACCCTGTA TTTGCACTTT AAGGGCTTTG ACCGCATTGA GAACCTGGAG 
GAGTACACGG GCCTGCGCTG CCTCTGGCTG GAGTGCAATG GCATCCAGAG GATCGAGAAT 
CTGCAGGCCC AGAGCGAACT GCGCTGCCTC TTCCTGCAAG TGAACCTGTT GCACAAGATC 
GAGAACCTGG AGCCCCTCCA GAAGCTGGAC GCCCTCAACC TGAGCAACAA CTACATCAAG 
ACCATTGAGA ACCTCTCCTG CCTCCCTGTC CTGAACACAC TGCAAATGGC CCACAACCGC 
CTGGAGACGG TGGCTGACAT TGAGCACCTC AGGGAGTGTC TGCGACTCTG TGTCCTCGAC 
CTCTCCCACA ATGCGCTGAG TGACCCTGAG ATCCTGAGCG TGCTAGAGAG CATGCCCTGT 
CTGCGTGTGC TGAACCTCAT GGGAAACCCA GTGACCAAAC ACATCCCTAA CTACCGCAGG 
ACGGTCACTG TCCGTCTGAA GCACTTGACT TATCTGGATG ACAGGCCAGT TTTCCCCAAG 
GACAGGGCGT GTGCAGAGGC ATGGGCCCGA GGTGGGTATG CCGCCGAGAA GGAAGAGCGT 
CGCCAGTGGG AGAGCAGGGA ACACAAGAAG ATAACAGACA GCCTGGAAGC CTTGGCCATG 
ATCAAGCGTC GGGCTGAGGA GCGGAAGAAG GCAAGAGATA GAGGGGAGAC ACCCCTGCCA 
GACAGCGAAG GGAGTATCCC CACCAGCCCG GAGGCTGAGG AAAAGCAACC CATGGGGGAA 
ATCCAGAAGA AGATGGAGTT GTTTGTGGAG GAGAGCTTTG AAGCCAAAGA TGAACTGTTT 
CCAGAGACGC CAGGAGGAGA AAAGGAGCTG CATGTGGTTG TGGTGAATGG GGCAGTGGAG 
AATCCAGACC TGTCTGGGAG CCTGGCTCAC AACCAGACAC CTGTGGTGGT CACCCCGGAA 
GAGTCTACAT CTCCAGTGGC AGCTACTGAT GGGGCCAGAA CTGAAGATAT AGAGGCCGTT 
GCCGTGGAGA TCAAGGAGAG GCTTTTCATT GATGACCTTC CTGACTTGGA AGATGCTGAG 
GGTACAGATG TGTCTGTGGA AGACCAGATA AAGGAGACAG ACATCCCGAG AATCCAGGCC 
ATTTCCAGCT TGAGTGACGA CAGTGACCCG GAGCTGGACG AGCTGTCCCT CTCCACGTCT 
GAGGCCACTC CCACAGGCGC CACAGGTGCC CTCTCACACA TATTTGCCAT CTCCAAGGGC 
CCCTCCACGG CAGCCACGGT GCCTTTCACA GACATATGTA AGCCAATAGC AACAACGGAC 
TTAGAAAGCC AAGGTCAAGA CTGCGGTGCA GCGGCCTCAC GCCCCCTCAT CCAGGAGCTC 
AATGATGAGC CGGCAGAGGA AGCAGCAAAC CAGCCACTGC CGCCCCAAAC CTGTGCGAGT 
GACCCTGCAC TTGCCCACCC TAGTGAAGAT GGAGACAGCG ACAGCCAGCT GCCTGCAGCC 
ACTCTGCTAG GAGATGGAGC GGAGGATGAA GCAGAGTCTT CGGTGCACCC GAAGGAGCCC 
AGCACCCGGG TAGGCCTAGA AGATATTGAA TTTGGCTTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice.
G3 (Bethesda, Md.)6(8)2479-87(2016 08)
Ha S,Lindsay AM,Timms AE,Beier DR

A forward genetic screen in mice identifies mutants with abnormal cortical patterning.
Cerebral cortex (New York, N.Y. : 1991)25(1)167-79(2015 Jan)
Ha S,Stottmann RW,Furley AJ,Beier DR

Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.
The Journal of cell biology204(2)203-13(2014 Jan)
Dong F,Shinohara K,Botilde Y,Nabeshima R,Asai Y,Fukumoto A,Hasegawa T,Matsuo M,Takeda H,Shiratori H,Nakamura T,Hamada H

Deletions and point mutations of LRRC50 cause primary ciliary dyskinesia due to dynein arm defects.
American journal of human genetics85(6)883-9(2009 Dec)
Loges NT,Olbrich H,Becker-Heck A,H?ffner K,Heer A,Reinhard C,Schmidts M,Kispert A,Zariwala MA,Leigh MW,Knowles MR,Zentgraf H,Seithe H,N?rnberg G,N?rnberg P,Reinhardt R,Omran H

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Dnaaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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