H2al2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	H2al2a
Entrez Gene ID	68231
Full Name	H2A histone family, member B1
Synonyms	1700113O17Rik,H2A.Bbd,H2A.L.2,H2al2,TSEG-1,Tseg1
General protein information	Preferred Names H2A histone family, member B1 Names histone H2A-Bbd type 1 H2A Barr body-deficient histone H2A-like 2 histone H2Alike 2 histone variant H2al2 testis-specific expressed gene 1 protein testis-specific expressed protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_026627.2	NP_080903.1	histone H2A-Bbd type 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC617633	XP_003588236.1
Mus musculus (house mouse)	Gm14484	NP_001020431.1
Mus musculus (house mouse)	Gm5132	NP_001078986.1
Mus musculus (house mouse)	Gm14476	NP_001229879.1
Canis lupus familiaris (dog)	LOC491818	XP_548938.1
Bos taurus (cattle)	LOC523631	NP_001071426.1
Mus musculus (house mouse)	Gm14474	NP_001229876.1
Mus musculus (house mouse)	Gm6026	NP_001171040.1
Canis lupus familiaris (dog)	LOC491827	XP_548947.1
Mus musculus (house mouse)	Gm14501	NP_001079006.1
Mus musculus (house mouse)	Gm14475	NP_001229883.1
Mus musculus (house mouse)	Gm14482	NP_001229881.1
Mus musculus (house mouse)	Gm14479	NP_001229880.1
Mus musculus (house mouse)	Gm4906	NP_001108001.1
Mus musculus (house mouse)	H2afb1	NP_080903.1
Rattus norvegicus (Norway rat)	LOC100363171	XP_002730244.1
Homo sapiens (human)	LOC101928761	XP_005274690.1
Mus musculus (house mouse)	Gm5382	NP_001029272.1
Mus musculus (house mouse)	Gm14483	NP_001104507.1
Mus musculus (house mouse)	Gm14478	NP_001229882.1
Mus musculus (house mouse)	Gm14477	NP_001229878.1
Mus musculus (house mouse)	Gm16501	NP_001106866.1
Mus musculus (house mouse)	1700012L04Rik	NP_083864.1

Gene Ontology
Bibliography

Related articles in PubMed

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
Barral S, Morozumi Y, Tanaka H, Montellier E, Govin J, de Dieuleveult M, Charbonnier G, Cout? Y, Puthier D, Buchou T, Boussouar F, Urahama T, Fenaille F, Curtet S, H?ry P, Fernandez-Nunez N, Shiota H, G?rard M, Rousseaux S, Kurumizaka H, Khochbin S
Molecular cell66(1)89-101.e8(2017 Apr)

Nucleosome adaptability conferred by sequence and structural variations in histone H2A-H2B dimers.
Shaytan AK, Landsman D, Panchenko AR
Current opinion in structural biology3248-57(2015 Jun)

Over-expression of testis-specific expressed gene 1 attenuates the proliferation and induces apoptosis of GC-1spg cells.
Gu CH, Tian FY, Pu JR, Zheng LD, Mei H, Zeng FQ, Yang JJ, Kan QC, Tong QS
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban34(4)535-41(2014 Aug)

The histone variant H2A.Bbd is enriched at sites of DNA synthesis.
Sansoni V, Casas-Delucchi CS, Rajan M, Schmidt A, B?nisch C, Thomae AW, Staege MS, Hake SB, Cardoso MC, Imhof A
Nucleic acids research42(10)6405-20(2014 Jun)

TSEG-1, a novel member of histone H2A variants, participates in spermatogenesis via promoting apoptosis of spermatogenic cells.
Gu C, Tong Q, Zheng L, Liang Z, Pu J, Mei H, Hu T, Du Z, Tian F, Zeng F
Genomics95(5)278-89(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The incorporation of H2A.L.2 initiates and orchestrates a series of successive transitional states that ultimately shift to the fully compacted genome of the mature spermatozoa.
Title: Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.

These results indicated over-expression of TSEG-1 suppresses the proliferation and induces the apoptosis of GC-1spg cells, which establishes a basis for further study on the function of TSEG-
Title: Over-expression of testis-specific expressed gene 1 attenuates the proliferation and induces apoptosis of GC-1spg cells.

The unusual properties of the H2AL2 nucleosomes suggest a specific role of H2AL2 during mouse spermiogenesis.
Title: The incorporation of the novel histone variant H2AL2 confers unusual structural and functional properties of the nucleosome.

Results indicated that TSEG-1 was cloned from X chromosome, with full-length sequence of 510 bp. The ORF is 336 bp that en-codes a deduced amino acid sequence of 111 residues. It was distinctively expressed in testis, but not in other tissues . : [TSEG-1]
Title: [Cloning and sequence analysis of TSEG-1, a novel gene specifically expressed in mouse testis].

H2AL1 and H2AL2, specifically mark the pericentric regions in condensing spermatids and participate in the formation of new nucleoprotein structures
Title: Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.

The following H2al2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H2al2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17952	NM_026627.2 Latest version!	Mus musculus H2A histone family member L2A (H2al2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H2al2a ( NM_026627.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080903.1 Mus musculus H2A histone family member L2A (H2al2a), mRNA.

Price & Availability↑

CloneID	OMu17952
Clone ID Related Accession (Same CDS sequence)	NM_026627.2
Accession Version	NM_026627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	histone H2A-Bbd type 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL928589.11. On Sep 17, 2015 this sequence version replaced NM_026627.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript is intronless :: BC061062.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-independent histone :: PMID: 25731851 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGA AAAGGCAAAG GCGAAGAAGA CGGAAAGTGA CCCGCTCCCA GAGAGCTGAG 
CTTCAATTTC CTGTTAGCCG TGTGGATCGT TTCCTACGAG AGGGAAACTA TTCTAGACGC 
CTGAGCTCTT CTGCTCCTGT ATTCCTCGCG GGTGTGCTCG AGTACTTAAC ATCTAACATC 
CTTGAACTGG CTGGTGAGGT GGCCCACACC ACTGGCAGGA AGCGCATAGC TCCAGAGCAT 
GTGTGCCGTG TGGTACAGAA CAACGAACAG CTCCACCAAC TCTTCAAACA AGGTGGCACA 
TCAGTGTTTG AGCCACCAGA ACCTGATGAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080903.1
CDS	86..421
Translation	MARKRQRRRRRKVTRSQRAELQFPVSRVDRFLREGNYSRRLSSSAPVFLAGVLEYLTSNILELAGEVAHTTGRKRIAPEHVCRVVQNNEQLHQLFKQGGTSVFEPPEPDDN

Target ORF information:

RefSeq Version	NM_026627.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus H2A histone family member L2A (H2al2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026627.2

ORF Insert Sequence:

ATGGCCAGGA AAAGGCAAAG GCGAAGAAGA CGGAAAGTGA CCCGCTCCCA GAGAGCTGAG 
CTTCAATTTC CTGTTAGCCG TGTGGATCGT TTCCTACGAG AGGGAAACTA TTCTAGACGC 
CTGAGCTCTT CTGCTCCTGT ATTCCTCGCG GGTGTGCTCG AGTACTTAAC ATCTAACATC 
CTTGAACTGG CTGGTGAGGT GGCCCACACC ACTGGCAGGA AGCGCATAGC TCCAGAGCAT 
GTGTGCCGTG TGGTACAGAA CAACGAACAG CTCCACCAAC TCTTCAAACA AGGTGGCACA 
TCAGTGTTTG AGCCACCAGA ACCTGATGAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
Molecular cell66(1)89-101.e8(2017 Apr)
Barral S,Morozumi Y,Tanaka H,Montellier E,Govin J,de Dieuleveult M,Charbonnier G,Cout? Y,Puthier D,Buchou T,Boussouar F,Urahama T,Fenaille F,Curtet S,H?ry P,Fernandez-Nunez N,Shiota H,G?rard M,Rousseaux S,Kurumizaka H,Khochbin S

Nucleosome adaptability conferred by sequence and structural variations in histone H2A-H2B dimers.
Current opinion in structural biology3248-57(2015 Jun)
Shaytan AK,Landsman D,Panchenko AR

Over-expression of testis-specific expressed gene 1 attenuates the proliferation and induces apoptosis of GC-1spg cells.
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban34(4)535-41(2014 Aug)
Gu CH,Tian FY,Pu JR,Zheng LD,Mei H,Zeng FQ,Yang JJ,Kan QC,Tong QS

The histone variant H2A.Bbd is enriched at sites of DNA synthesis.
Nucleic acids research42(10)6405-20(2014 Jun)
Sansoni V,Casas-Delucchi CS,Rajan M,Schmidt A,B?nisch C,Thomae AW,Staege MS,Hake SB,Cardoso MC,Imhof A

TSEG-1, a novel member of histone H2A variants, participates in spermatogenesis via promoting apoptosis of spermatogenic cells.
Genomics95(5)278-89(2010 May)
Gu C,Tong Q,Zheng L,Liang Z,Pu J,Mei H,Hu T,Du Z,Tian F,Zeng F

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H2al2a cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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