Cgnl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cgnl1
Entrez Gene ID	68178
Full Name	cingulin-like 1
Synonyms	4933421H10Rik,9930020M10Rik,AI503810,Jacop
General protein information	Preferred Names cingulin-like 1 Names cingulin-like protein 1 junction-associated coiled-coil protein paracingulin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 D
Summary	This gene encodes a protein localized to the tight junctions and adherens junctions in vertebrate epithelial cells. The encoded protein regulates the activity of Rho family GTPases during junction assembly and at confluence. At the adherens junctions, the encoded protein is part of a protein complex that links E-cadherin to the microtubule cytoskeleton. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001304362.1	NP_001291291.1	cingulin-like protein 1
NM_026599.5	NP_080875.3	cingulin-like protein 1
XM_006511395.1	XP_006511458.1	cingulin-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CGNL1	NP_116255.2
Danio rerio (zebrafish)	cgnl1	XP_003199047.1
Gallus gallus (chicken)	CGNL1	XP_413790.4
	CGNL1	XP_001092704.1
Mus musculus (house mouse)	Cgnl1	NP_080875.3
Pan troglodytes (chimpanzee)	CGNL1	XP_510437.2
Bos taurus (cattle)	CGNL1	XP_005211807.1
Xenopus tropicalis (tropical clawed frog)	cgnl1	NP_001120551.1
Canis lupus familiaris (dog)	CGNL1	XP_005638512.1
Rattus norvegicus (Norway rat)	Cgnl1	NP_001101634.1

Gene Ontology
Bibliography

Related articles in PubMed

Cgnl1, an endothelial junction complex protein, regulates GTPase mediated angiogenesis.
Chrifi I, Hermkens D, Brandt MM, van Dijk CGM, B?rgisser PE, Haasdijk R, Pei J, van de Kamp EHM, Zhu C, Blonden L, Kros JM, Duncker DJ, Duckers HJ, Cheng C
Cardiovascular research113(14)1776-1788(2017 Dec)

Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
Guillemot L, Schneider Y, Brun P, Castagliuolo I, Pizzuti D, Martines D, Jond L, Bongiovanni M, Citi S
Journal of cell science125(Pt 21)5005-14(2012 Nov)

Cingulin, paracingulin, and PLEKHA7: signaling and cytoskeletal adaptors at the apical junctional complex.
Citi S, Pulimeno P, Paschoud S
Annals of the New York Academy of Sciences1257125-32(2012 Jun)

Endocardiogenesis in embryoid bodies: novel markers identified by gene expression profiling.
Narumiya H, Hidaka K, Shirai M, Terami H, Aburatani H, Morisaki T
Biochemical and biophysical research communications357(4)896-902(2007 Jun)

JACOP, a novel plaque protein localizing at the apical junctional complex with sequence similarity to cingulin.
Ohnishi H, Nakahara T, Furuse K, Sasaki H, Tsukita S, Furuse M
The Journal of biological chemistry279(44)46014-22(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

JACOP is involved in anchoring the apical junctional complex, especially TJs, to actin-based cytoskeletons.
Title: JACOP, a novel plaque protein localizing at the apical junctional complex with sequence similarity to cingulin.

The following Cgnl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cgnl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02649	NM_026599.5 Latest version!	Mus musculus cingulin-like 1 (Cgnl1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu02649	NM_001304362.1 Latest version!	Mus musculus cingulin-like 1 (Cgnl1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu02649	XM_006511395.1 Latest version!	Mus musculus cingulin-like 1 (Cgnl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cgnl1 ( NM_001304362.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291291.1 Mus musculus cingulin-like 1 (Cgnl1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02649
Clone ID Related Accession (Same CDS sequence)	NM_001304362.1 , NM_026599.5 , XM_006511395.1
Accession Version	NM_001304362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	cingulin-like protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB629895.1, AC093483.6, AK040774.1, AK173252.1, BB667316.1 and CJ252240.1. On Feb 16, 2015 this sequence version replaced XM_006511394.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB186125.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGT ATTTTGGCGA ATACCAACAC GTACAGCAGG AGTACGGGGT ACACCTGAGA 
CTTGCCAGTG GTGACACTCC AAAGCCAAGG AATTCCCAGC CCTCAAAGGC AGGCTCCTAT 
GGTGTCAGCA TCAGGGTCCA GGGAATTGAT GGCCACCCTT ATATAGTCCT GAATAACACT 
GAGCGGTGTC TAGCAGGCAC ACCTTTCCCA GAAAACGCGC CATCGTTTCC ATCTTCAGTG 
ATAAATAACC TGTCCCTACA TCCAAGCAAT GGAACCGTGT TGAAGGAGAA CACTCCGGAG 
GAACTGCAGC TTCCAGAAAA CCCGTACCTG CAAACCAGCC CGCTAAGAGG CCAGAAGCAG 
TTCTCCCTCC ACGAGGGCAG GAATGGAGTT CTAGAACGCA AAGACGGGCC CACGAAGCTG 
CCCCATGTGC TCAACTTCCA GAGGCACCCG GAGCTTCTGC AGCCCTATGA CCCAGAAAAG 
AATGAGGTGA ACGCAAAGAA GCATCACCCT CCCGAGAGTC CCTGGCTGAG AAATGCAACA 
GAGGACGGGA CCAACTGTAA GAAGTCCCGG AACTGCTTCC CCAAATCCTA CGGTTCCCAG 
CCCAACAGCC CTACTTCGGA AGATTTAGCC AAGACCAACA TGACAGCGAT CCGTCTCTGC 
AGCTCTGTGG TCATAGAAGA CCCCCAAAAG CAGACCTCAG TGTGCGTGAA CGTTCAGAGG 
TGCGCCAAGG AGGGGGTGGG AGAGGAGACC CTTTCCCCTC GACGGAAATC CCCAACTGCC 
CCCAGCCCAC AGGCCTATTC TGAAACCAAG AAAAACAGGC CAGATGTGCT ACCCTTCCGG 
CGACAAGATT CCGCAGGACC CATCCTGGAT GGAGCTCGGT CACGCAGGTC CTCTTCGTCA 
TCCACGACTC CTACTTCTGC CACTTCCTTG TACAAATTTT TACTTGATGA TCAGGAATGC 
GCCATTCATG CCGACAGCGT CAACCGCCAT GAAAACAGAA GGTATATTCC CTTCTTGCCA 
GGAACTGGGC GGGATATCGA CACCTGCTCA ATTCCTGGTG TGGACCAGTT AATTGAAAAA 
TTTGACCAAA AGCCTGGCCT TCAGAGAAGA GGAAGGTCTG GGAAGAGAAA CAGGATCAAT 
CCCGACGACA GGAAAAGATC CCGAAGTGTA GATAGTGCCT TTCCATTTGG TCTCCAGGGA 
AACACAGAAT ACCTCACCGA GTTCAGCAGG AACCTGGGCA AGTCTAGCGA ACACCTCCTT 
CGGCCATCCC AGGTCTTCCC GCAGAGGTCA GTGGCCCAGG AGCACCGTGG GAAGCACAGT 
CCCAGCAGCC CGCCCGCAAA GCTGCAGGGC GCTCAAGGTG CCCACCCCAA GCCTCCCCTG 
CAGAACAAAG ATGGGAAAGT TCTGAACAAA GGAAGGCAGG AAAGCACGGG GGCATGTGCC 
CCCTCCCTGC CAGCACCGAA TAAAAAAGAG GAAGAAATCA AAATAGCCAC TGCCACGTTG 
ATGTTACAGA ACCGGGCGGT GGCTGCCACA TCGGATTCTG GTGCCAAGAA AATTTCAGTG 
AAGACATTTC CTTCCGACTC CAGCACTCAG GCCACACCAG ATCTCTTAAA GGGCCAGCAG 
GAGCTCACAC AACAAACGAA CGAGGAGACC GCCAAGCAGA TCCTTTACAA TTACCTCAAA 
GAAGGAGGCA CAGACAATGA GGATGCCACC AAAAGGAAAG TGAACCTGGT CTTTGAGAAA 
ATCCAGACTT TGAAGTCCAG AGCAGCAGGG AGTGCCCAAG GAAGCAATCA AGCTCCGAAT 
TCTCCCTCTG AAGGCAACAG TCTATTGGAT CAGAAGAACA AATTGATCCT AGAAGTGTCT 
GAACTACAGC AGCAACTGCA ACTGGAGATG AAGAACCAAC AGAACATCAA GGAGGAGCGA 
GAAAGAATGA GAGAGGATTT AGAAGAGCTG CGAGTCCGGC ACCAGAGCCA AGTGGAGGAG 
ACGGCCACCC TGCAGAGACG GCTGGAGGAA AGTGAAGGCG AGCTGAGGAA GAGCTTGGAA 
GAGCTGTTCC AAGTGAAGAT GGAGCGTGAA CAGCACCAGA CTGAGATCAG AGACCTGCAG 
GACCAGCTGT CGGAAATGCA TGATGAGCTA GACAGCACCA AGCGATCCGA GGACAGGGAG 
AAGGGAGCCC TGATTGAGGA GCTCCTGCAA GCCAAGCAGG ACCTTCAAGA TCTGCTCATT 
GCCAAAGAGG AACAGGAAGA TCTCTTGAGG AAGCGGGAGC GGGAGCTGAC TGCCTTGAAG 
GGCGCCTTGA AGGAGGAGGT TTCCAGCCAT GACCAGGAGA TGGATAAGCT GAAGGAACAG 
TACGATGCTG AGCTGCAGGC CCTCAGGGAG AGTGTGGAGG AGGCAACCAA GAATGTTGAG 
GTCCTGGCCA GCAGGAGCAA CTCCTCAGAG CAGAGCCAGG CGGAGGCTGA CCTGCGTGAG 
AAAGTGCTGA AGGAGGAGAA TGAGAAGCTG CAGGGGAGGA TTGCGGAGCT GGAGCGCAGG 
GCAGCCCAGC TACAGAGGCA GATGGAGGAT GTGAAGGGTG ACGAGGCCCA AGCAAAGGAG 
ACACTCCGAA AGTGTGAGAG TGAAGTACAG CAGCTGGAGG AAGCCCTTGT GCATGCCAGA 
AAGGAAGAAA AAGAAGCCAC ATGTGCCAGA AGGGCGCTGG AGAAGGAGCT GGAGCAAGCT 
CGGAGAGAGC TGAGCCAGGT CTCCCAGGAG CAGAAGGAAC TCCTGGAGAA ACTCCGGGAC 
GAGGCGGAGC AGAAGGAGCA GCTGCGAAAG CTGAAGAACG AGATGGAGAG CGAGCGGTGG 
CACCTGGACA AGACCATCGA GAAGCTGCAG AAGGAGATGG CCGACATTGC TGAGGCCTCC 
CGCACATCCT CTTTGGAGCT GCAGAAGCAG CTGGGCGAGT ATAAGGAGAA GAACCGCCGG 
GAGCTTGCGG AAATGCAGAC ACAACTGAAG GAGAAATGCC TGGAAGTGGA AAAGGCCAGA 
CTGGCAGCCT CGAAGATGCA GGATGAGTTA CGCCTCAAGG AGGAAGAGTT GCAGGACTAC 
CAGCGAGCTG AGGAGGAGGC GCTCACAAAA CGGCAGCTTC TGGAGCAGTC GCTGAAGGAC 
CTGGAGTATG AGCTGGAGGC CAAGAGCCAC CTCAAGGATG ACCGCAGCCG ACTCATCAAG 
CAGATGGAGG ACAAGGTGTC CCAATTGGAG ATTGAATTGG AAGAAGAAAG AACCAACGCT 
GACTTGCTGT CGGAGAGGAT CACGTGGAGC AGGGAACAGA TGGAGCAGAT GAGGAGCGAG 
CTTCTTCAGG AGAAAGCTGC CAAGCAAGAC TTAGAGTGTG ACAAGATTTC CCTAGAGAGA 
CAGAACAAAG ACTTAAAGAG CCGGATTATC CACCTGGAAG GTTCCTACAG GTCCAGCAAG 
GAGGGCCTGG TGGTGCAGAT GGAGGCCAGG ATTGCAGAGC TGGAGGACCG GCTGGAGAAT 
GAGGAGAGGG ATCGGGCCAA CCTGCAGCTC AGCAACCGAA GGCTGGAGCG GAAGGTGAAG 
GAGCTGGTGA TGCAGGTGGA TGATGAGCAT CTGTCACTGA CGGACCAGAA GGACCAGCTG 
AGCTTGCGCC TGAAAGCCAT GAAGCGCCAG GTGGAGGAGG CTGAAGAGGA GATCGACCGA 
CTGGAAAGTT CTAAGAAGAA GCTGCAGAGG GAGCTGGAGG AGCAGATGGG GGTGAATGAG 
CAGCTGCAGG GGCAGCTCAA CTCTCTGAAG AAGGGCTTAA GGCTTAAGAC ACTGTCTAGT 
AAAGTACTGG ACGACTCGGA CGACGACGAC CTCAGCAGTG ATGCGGGAAG CCTCTACGAA 
GCCCCACTGA GCTATGCCTT TCCCAAAGAC AGCACTATCG CCAGCCAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291291.1
CDS	587..4480
Translation	MELYFGEYQHVQQEYGVHLRLASGDTPKPRNSQPSKAGSYGVSIRVQGIDGHPYIVLNNTERCLAGTPFPENAPSFPSSVINNLSLHPSNGTVLKENTPEELQLPENPYLQTSPLRGQKQFSLHEGRNGVLERKDGPTKLPHVLNFQRHPELLQPYDPEKNEVNAKKHHPPESPWLRNATEDGTNCKKSRNCFPKSYGSQPNSPTSEDLAKTNMTAIRLCSSVVIEDPQKQTSVCVNVQRCAKEGVGEETLSPRRKSPTAPSPQAYSETKKNRPDVLPFRRQDSAGPILDGARSRRSSSSSTTPTSATSLYKFLLDDQECAIHADSVNRHENRRYIPFLPGTGRDIDTCSIPGVDQLIEKFDQKPGLQRRGRSGKRNRINPDDRKRSRSVDSAFPFGLQGNTEYLTEFSRNLGKSSEHLLRPSQVFPQRSVAQEHRGKHSPSSPPAKLQGAQGAHPKPPLQNKDGKVLNKGRQESTGACAPSLPAPNKKEEEIKIATATLMLQNRAVAATSDSGAKKISVKTFPSDSSTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGGTDNEDATKRKVNLVFEKIQTLKSRAAGSAQGSNQAPNSPSEGNSLLDQKNKLILEVSELQQQLQLEMKNQQNIKEERERMREDLEELRVRHQSQVEETATLQRRLEESEGELRKSLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSTKRSEDREKGALIEELLQAKQDLQDLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDQEMDKLKEQYDAELQALRESVEEATKNVEVLASRSNSSEQSQAEADLREKVLKEENEKLQGRIAELERRAAQLQRQMEDVKGDEAQAKETLRKCESEVQQLEEALVHARKEEKEATCARRALEKELEQARRELSQVSQEQKELLEKLRDEAEQKEQLRKLKNEMESERWHLDKTIEKLQKEMADIAEASRTSSLELQKQLGEYKEKNRRELAEMQTQLKEKCLEVEKARLAASKMQDELRLKEEELQDYQRAEEEALTKRQLLEQSLKDLEYELEAKSHLKDDRSRLIKQMEDKVSQLEIELEEERTNADLLSERITWSREQMEQMRSELLQEKAAKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLENEERDRANLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMGVNEQLQGQLNSLKKGLRLKTLSSKVLDDSDDDDLSSDAGSLYEAPLSYAFPKDSTIASQI

Target ORF information:

RefSeq Version	NM_001304362.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cingulin-like 1 (Cgnl1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304362.1

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGCGA ATACCAACAC GTACAGCAGG AGTACGGGGT ACACCTGAGA 
CTTGCCAGTG GTGACACTCC AAAGCCAAGG AATTCCCAGC CCTCAAAGGC AGGCTCCTAT 
GGTGTCAGCA TCAGGGTCCA GGGAATTGAT GGCCACCCTT ATATAGTCCT GAATAACACT 
GAGCGGTGTC TAGCAGGCAC ACCTTTCCCA GAAAACGCGC CATCGTTTCC ATCTTCAGTG 
ATAAATAACC TGTCCCTACA TCCAAGCAAT GGAACCGTGT TGAAGGAGAA CACTCCGGAG 
GAACTGCAGC TTCCAGAAAA CCCGTACCTG CAAACCAGCC CGCTAAGAGG CCAGAAGCAG 
TTCTCCCTCC ACGAGGGCAG GAATGGAGTT CTAGAACGCA AAGACGGGCC CACGAAGCTG 
CCCCATGTGC TCAACTTCCA GAGGCACCCG GAGCTTCTGC AGCCCTATGA CCCAGAAAAG 
AATGAGGTGA ACGCAAAGAA GCATCACCCT CCCGAGAGTC CCTGGCTGAG AAATGCAACA 
GAGGACGGGA CCAACTGTAA GAAGTCCCGG AACTGCTTCC CCAAATCCTA CGGTTCCCAG 
CCCAACAGCC CTACTTCGGA AGATTTAGCC AAGACCAACA TGACAGCGAT CCGTCTCTGC 
AGCTCTGTGG TCATAGAAGA CCCCCAAAAG CAGACCTCAG TGTGCGTGAA CGTTCAGAGG 
TGCGCCAAGG AGGGGGTGGG AGAGGAGACC CTTTCCCCTC GACGGAAATC CCCAACTGCC 
CCCAGCCCAC AGGCCTATTC TGAAACCAAG AAAAACAGGC CAGATGTGCT ACCCTTCCGG 
CGACAAGATT CCGCAGGACC CATCCTGGAT GGAGCTCGGT CACGCAGGTC CTCTTCGTCA 
TCCACGACTC CTACTTCTGC CACTTCCTTG TACAAATTTT TACTTGATGA TCAGGAATGC 
GCCATTCATG CCGACAGCGT CAACCGCCAT GAAAACAGAA GGTATATTCC CTTCTTGCCA 
GGAACTGGGC GGGATATCGA CACCTGCTCA ATTCCTGGTG TGGACCAGTT AATTGAAAAA 
TTTGACCAAA AGCCTGGCCT TCAGAGAAGA GGAAGGTCTG GGAAGAGAAA CAGGATCAAT 
CCCGACGACA GGAAAAGATC CCGAAGTGTA GATAGTGCCT TTCCATTTGG TCTCCAGGGA 
AACACAGAAT ACCTCACCGA GTTCAGCAGG AACCTGGGCA AGTCTAGCGA ACACCTCCTT 
CGGCCATCCC AGGTCTTCCC GCAGAGGTCA GTGGCCCAGG AGCACCGTGG GAAGCACAGT 
CCCAGCAGCC CGCCCGCAAA GCTGCAGGGC GCTCAAGGTG CCCACCCCAA GCCTCCCCTG 
CAGAACAAAG ATGGGAAAGT TCTGAACAAA GGAAGGCAGG AAAGCACGGG GGCATGTGCC 
CCCTCCCTGC CAGCACCGAA TAAAAAAGAG GAAGAAATCA AAATAGCCAC TGCCACGTTG 
ATGTTACAGA ACCGGGCGGT GGCTGCCACA TCGGATTCTG GTGCCAAGAA AATTTCAGTG 
AAGACATTTC CTTCCGACTC CAGCACTCAG GCCACACCAG ATCTCTTAAA GGGCCAGCAG 
GAGCTCACAC AACAAACGAA CGAGGAGACC GCCAAGCAGA TCCTTTACAA TTACCTCAAA 
GAAGGAGGCA CAGACAATGA GGATGCCACC AAAAGGAAAG TGAACCTGGT CTTTGAGAAA 
ATCCAGACTT TGAAGTCCAG AGCAGCAGGG AGTGCCCAAG GAAGCAATCA AGCTCCGAAT 
TCTCCCTCTG AAGGCAACAG TCTATTGGAT CAGAAGAACA AATTGATCCT AGAAGTGTCT 
GAACTACAGC AGCAACTGCA ACTGGAGATG AAGAACCAAC AGAACATCAA GGAGGAGCGA 
GAAAGAATGA GAGAGGATTT AGAAGAGCTG CGAGTCCGGC ACCAGAGCCA AGTGGAGGAG 
ACGGCCACCC TGCAGAGACG GCTGGAGGAA AGTGAAGGCG AGCTGAGGAA GAGCTTGGAA 
GAGCTGTTCC AAGTGAAGAT GGAGCGTGAA CAGCACCAGA CTGAGATCAG AGACCTGCAG 
GACCAGCTGT CGGAAATGCA TGATGAGCTA GACAGCACCA AGCGATCCGA GGACAGGGAG 
AAGGGAGCCC TGATTGAGGA GCTCCTGCAA GCCAAGCAGG ACCTTCAAGA TCTGCTCATT 
GCCAAAGAGG AACAGGAAGA TCTCTTGAGG AAGCGGGAGC GGGAGCTGAC TGCCTTGAAG 
GGCGCCTTGA AGGAGGAGGT TTCCAGCCAT GACCAGGAGA TGGATAAGCT GAAGGAACAG 
TACGATGCTG AGCTGCAGGC CCTCAGGGAG AGTGTGGAGG AGGCAACCAA GAATGTTGAG 
GTCCTGGCCA GCAGGAGCAA CTCCTCAGAG CAGAGCCAGG CGGAGGCTGA CCTGCGTGAG 
AAAGTGCTGA AGGAGGAGAA TGAGAAGCTG CAGGGGAGGA TTGCGGAGCT GGAGCGCAGG 
GCAGCCCAGC TACAGAGGCA GATGGAGGAT GTGAAGGGTG ACGAGGCCCA AGCAAAGGAG 
ACACTCCGAA AGTGTGAGAG TGAAGTACAG CAGCTGGAGG AAGCCCTTGT GCATGCCAGA 
AAGGAAGAAA AAGAAGCCAC ATGTGCCAGA AGGGCGCTGG AGAAGGAGCT GGAGCAAGCT 
CGGAGAGAGC TGAGCCAGGT CTCCCAGGAG CAGAAGGAAC TCCTGGAGAA ACTCCGGGAC 
GAGGCGGAGC AGAAGGAGCA GCTGCGAAAG CTGAAGAACG AGATGGAGAG CGAGCGGTGG 
CACCTGGACA AGACCATCGA GAAGCTGCAG AAGGAGATGG CCGACATTGC TGAGGCCTCC 
CGCACATCCT CTTTGGAGCT GCAGAAGCAG CTGGGCGAGT ATAAGGAGAA GAACCGCCGG 
GAGCTTGCGG AAATGCAGAC ACAACTGAAG GAGAAATGCC TGGAAGTGGA AAAGGCCAGA 
CTGGCAGCCT CGAAGATGCA GGATGAGTTA CGCCTCAAGG AGGAAGAGTT GCAGGACTAC 
CAGCGAGCTG AGGAGGAGGC GCTCACAAAA CGGCAGCTTC TGGAGCAGTC GCTGAAGGAC 
CTGGAGTATG AGCTGGAGGC CAAGAGCCAC CTCAAGGATG ACCGCAGCCG ACTCATCAAG 
CAGATGGAGG ACAAGGTGTC CCAATTGGAG ATTGAATTGG AAGAAGAAAG AACCAACGCT 
GACTTGCTGT CGGAGAGGAT CACGTGGAGC AGGGAACAGA TGGAGCAGAT GAGGAGCGAG 
CTTCTTCAGG AGAAAGCTGC CAAGCAAGAC TTAGAGTGTG ACAAGATTTC CCTAGAGAGA 
CAGAACAAAG ACTTAAAGAG CCGGATTATC CACCTGGAAG GTTCCTACAG GTCCAGCAAG 
GAGGGCCTGG TGGTGCAGAT GGAGGCCAGG ATTGCAGAGC TGGAGGACCG GCTGGAGAAT 
GAGGAGAGGG ATCGGGCCAA CCTGCAGCTC AGCAACCGAA GGCTGGAGCG GAAGGTGAAG 
GAGCTGGTGA TGCAGGTGGA TGATGAGCAT CTGTCACTGA CGGACCAGAA GGACCAGCTG 
AGCTTGCGCC TGAAAGCCAT GAAGCGCCAG GTGGAGGAGG CTGAAGAGGA GATCGACCGA 
CTGGAAAGTT CTAAGAAGAA GCTGCAGAGG GAGCTGGAGG AGCAGATGGG GGTGAATGAG 
CAGCTGCAGG GGCAGCTCAA CTCTCTGAAG AAGGGCTTAA GGCTTAAGAC ACTGTCTAGT 
AAAGTACTGG ACGACTCGGA CGACGACGAC CTCAGCAGTG ATGCGGGAAG CCTCTACGAA 
GCCCCACTGA GCTATGCCTT TCCCAAAGAC AGCACTATCG CCAGCCAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cgnl1 ( NM_026599.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080875.3 Mus musculus cingulin-like 1 (Cgnl1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu02649
Clone ID Related Accession (Same CDS sequence)	NM_001304362.1 , NM_026599.5 , XM_006511395.1
Accession Version	NM_026599.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	cingulin-like protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB629895.1, AK040774.1, AK173252.1, BB667316.1 and CJ252240.1. On Jan 22, 2015 this sequence version replaced NM_026599.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## CDS exon combination :: AB186125.1, BC158061.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGT ATTTTGGCGA ATACCAACAC GTACAGCAGG AGTACGGGGT ACACCTGAGA 
CTTGCCAGTG GTGACACTCC AAAGCCAAGG AATTCCCAGC CCTCAAAGGC AGGCTCCTAT 
GGTGTCAGCA TCAGGGTCCA GGGAATTGAT GGCCACCCTT ATATAGTCCT GAATAACACT 
GAGCGGTGTC TAGCAGGCAC ACCTTTCCCA GAAAACGCGC CATCGTTTCC ATCTTCAGTG 
ATAAATAACC TGTCCCTACA TCCAAGCAAT GGAACCGTGT TGAAGGAGAA CACTCCGGAG 
GAACTGCAGC TTCCAGAAAA CCCGTACCTG CAAACCAGCC CGCTAAGAGG CCAGAAGCAG 
TTCTCCCTCC ACGAGGGCAG GAATGGAGTT CTAGAACGCA AAGACGGGCC CACGAAGCTG 
CCCCATGTGC TCAACTTCCA GAGGCACCCG GAGCTTCTGC AGCCCTATGA CCCAGAAAAG 
AATGAGGTGA ACGCAAAGAA GCATCACCCT CCCGAGAGTC CCTGGCTGAG AAATGCAACA 
GAGGACGGGA CCAACTGTAA GAAGTCCCGG AACTGCTTCC CCAAATCCTA CGGTTCCCAG 
CCCAACAGCC CTACTTCGGA AGATTTAGCC AAGACCAACA TGACAGCGAT CCGTCTCTGC 
AGCTCTGTGG TCATAGAAGA CCCCCAAAAG CAGACCTCAG TGTGCGTGAA CGTTCAGAGG 
TGCGCCAAGG AGGGGGTGGG AGAGGAGACC CTTTCCCCTC GACGGAAATC CCCAACTGCC 
CCCAGCCCAC AGGCCTATTC TGAAACCAAG AAAAACAGGC CAGATGTGCT ACCCTTCCGG 
CGACAAGATT CCGCAGGACC CATCCTGGAT GGAGCTCGGT CACGCAGGTC CTCTTCGTCA 
TCCACGACTC CTACTTCTGC CACTTCCTTG TACAAATTTT TACTTGATGA TCAGGAATGC 
GCCATTCATG CCGACAGCGT CAACCGCCAT GAAAACAGAA GGTATATTCC CTTCTTGCCA 
GGAACTGGGC GGGATATCGA CACCTGCTCA ATTCCTGGTG TGGACCAGTT AATTGAAAAA 
TTTGACCAAA AGCCTGGCCT TCAGAGAAGA GGAAGGTCTG GGAAGAGAAA CAGGATCAAT 
CCCGACGACA GGAAAAGATC CCGAAGTGTA GATAGTGCCT TTCCATTTGG TCTCCAGGGA 
AACACAGAAT ACCTCACCGA GTTCAGCAGG AACCTGGGCA AGTCTAGCGA ACACCTCCTT 
CGGCCATCCC AGGTCTTCCC GCAGAGGTCA GTGGCCCAGG AGCACCGTGG GAAGCACAGT 
CCCAGCAGCC CGCCCGCAAA GCTGCAGGGC GCTCAAGGTG CCCACCCCAA GCCTCCCCTG 
CAGAACAAAG ATGGGAAAGT TCTGAACAAA GGAAGGCAGG AAAGCACGGG GGCATGTGCC 
CCCTCCCTGC CAGCACCGAA TAAAAAAGAG GAAGAAATCA AAATAGCCAC TGCCACGTTG 
ATGTTACAGA ACCGGGCGGT GGCTGCCACA TCGGATTCTG GTGCCAAGAA AATTTCAGTG 
AAGACATTTC CTTCCGACTC CAGCACTCAG GCCACACCAG ATCTCTTAAA GGGCCAGCAG 
GAGCTCACAC AACAAACGAA CGAGGAGACC GCCAAGCAGA TCCTTTACAA TTACCTCAAA 
GAAGGAGGCA CAGACAATGA GGATGCCACC AAAAGGAAAG TGAACCTGGT CTTTGAGAAA 
ATCCAGACTT TGAAGTCCAG AGCAGCAGGG AGTGCCCAAG GAAGCAATCA AGCTCCGAAT 
TCTCCCTCTG AAGGCAACAG TCTATTGGAT CAGAAGAACA AATTGATCCT AGAAGTGTCT 
GAACTACAGC AGCAACTGCA ACTGGAGATG AAGAACCAAC AGAACATCAA GGAGGAGCGA 
GAAAGAATGA GAGAGGATTT AGAAGAGCTG CGAGTCCGGC ACCAGAGCCA AGTGGAGGAG 
ACGGCCACCC TGCAGAGACG GCTGGAGGAA AGTGAAGGCG AGCTGAGGAA GAGCTTGGAA 
GAGCTGTTCC AAGTGAAGAT GGAGCGTGAA CAGCACCAGA CTGAGATCAG AGACCTGCAG 
GACCAGCTGT CGGAAATGCA TGATGAGCTA GACAGCACCA AGCGATCCGA GGACAGGGAG 
AAGGGAGCCC TGATTGAGGA GCTCCTGCAA GCCAAGCAGG ACCTTCAAGA TCTGCTCATT 
GCCAAAGAGG AACAGGAAGA TCTCTTGAGG AAGCGGGAGC GGGAGCTGAC TGCCTTGAAG 
GGCGCCTTGA AGGAGGAGGT TTCCAGCCAT GACCAGGAGA TGGATAAGCT GAAGGAACAG 
TACGATGCTG AGCTGCAGGC CCTCAGGGAG AGTGTGGAGG AGGCAACCAA GAATGTTGAG 
GTCCTGGCCA GCAGGAGCAA CTCCTCAGAG CAGAGCCAGG CGGAGGCTGA CCTGCGTGAG 
AAAGTGCTGA AGGAGGAGAA TGAGAAGCTG CAGGGGAGGA TTGCGGAGCT GGAGCGCAGG 
GCAGCCCAGC TACAGAGGCA GATGGAGGAT GTGAAGGGTG ACGAGGCCCA AGCAAAGGAG 
ACACTCCGAA AGTGTGAGAG TGAAGTACAG CAGCTGGAGG AAGCCCTTGT GCATGCCAGA 
AAGGAAGAAA AAGAAGCCAC ATGTGCCAGA AGGGCGCTGG AGAAGGAGCT GGAGCAAGCT 
CGGAGAGAGC TGAGCCAGGT CTCCCAGGAG CAGAAGGAAC TCCTGGAGAA ACTCCGGGAC 
GAGGCGGAGC AGAAGGAGCA GCTGCGAAAG CTGAAGAACG AGATGGAGAG CGAGCGGTGG 
CACCTGGACA AGACCATCGA GAAGCTGCAG AAGGAGATGG CCGACATTGC TGAGGCCTCC 
CGCACATCCT CTTTGGAGCT GCAGAAGCAG CTGGGCGAGT ATAAGGAGAA GAACCGCCGG 
GAGCTTGCGG AAATGCAGAC ACAACTGAAG GAGAAATGCC TGGAAGTGGA AAAGGCCAGA 
CTGGCAGCCT CGAAGATGCA GGATGAGTTA CGCCTCAAGG AGGAAGAGTT GCAGGACTAC 
CAGCGAGCTG AGGAGGAGGC GCTCACAAAA CGGCAGCTTC TGGAGCAGTC GCTGAAGGAC 
CTGGAGTATG AGCTGGAGGC CAAGAGCCAC CTCAAGGATG ACCGCAGCCG ACTCATCAAG 
CAGATGGAGG ACAAGGTGTC CCAATTGGAG ATTGAATTGG AAGAAGAAAG AACCAACGCT 
GACTTGCTGT CGGAGAGGAT CACGTGGAGC AGGGAACAGA TGGAGCAGAT GAGGAGCGAG 
CTTCTTCAGG AGAAAGCTGC CAAGCAAGAC TTAGAGTGTG ACAAGATTTC CCTAGAGAGA 
CAGAACAAAG ACTTAAAGAG CCGGATTATC CACCTGGAAG GTTCCTACAG GTCCAGCAAG 
GAGGGCCTGG TGGTGCAGAT GGAGGCCAGG ATTGCAGAGC TGGAGGACCG GCTGGAGAAT 
GAGGAGAGGG ATCGGGCCAA CCTGCAGCTC AGCAACCGAA GGCTGGAGCG GAAGGTGAAG 
GAGCTGGTGA TGCAGGTGGA TGATGAGCAT CTGTCACTGA CGGACCAGAA GGACCAGCTG 
AGCTTGCGCC TGAAAGCCAT GAAGCGCCAG GTGGAGGAGG CTGAAGAGGA GATCGACCGA 
CTGGAAAGTT CTAAGAAGAA GCTGCAGAGG GAGCTGGAGG AGCAGATGGG GGTGAATGAG 
CAGCTGCAGG GGCAGCTCAA CTCTCTGAAG AAGGGCTTAA GGCTTAAGAC ACTGTCTAGT 
AAAGTACTGG ACGACTCGGA CGACGACGAC CTCAGCAGTG ATGCGGGAAG CCTCTACGAA 
GCCCCACTGA GCTATGCCTT TCCCAAAGAC AGCACTATCG CCAGCCAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080875.3
CDS	110..4003
Translation	MELYFGEYQHVQQEYGVHLRLASGDTPKPRNSQPSKAGSYGVSIRVQGIDGHPYIVLNNTERCLAGTPFPENAPSFPSSVINNLSLHPSNGTVLKENTPEELQLPENPYLQTSPLRGQKQFSLHEGRNGVLERKDGPTKLPHVLNFQRHPELLQPYDPEKNEVNAKKHHPPESPWLRNATEDGTNCKKSRNCFPKSYGSQPNSPTSEDLAKTNMTAIRLCSSVVIEDPQKQTSVCVNVQRCAKEGVGEETLSPRRKSPTAPSPQAYSETKKNRPDVLPFRRQDSAGPILDGARSRRSSSSSTTPTSATSLYKFLLDDQECAIHADSVNRHENRRYIPFLPGTGRDIDTCSIPGVDQLIEKFDQKPGLQRRGRSGKRNRINPDDRKRSRSVDSAFPFGLQGNTEYLTEFSRNLGKSSEHLLRPSQVFPQRSVAQEHRGKHSPSSPPAKLQGAQGAHPKPPLQNKDGKVLNKGRQESTGACAPSLPAPNKKEEEIKIATATLMLQNRAVAATSDSGAKKISVKTFPSDSSTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGGTDNEDATKRKVNLVFEKIQTLKSRAAGSAQGSNQAPNSPSEGNSLLDQKNKLILEVSELQQQLQLEMKNQQNIKEERERMREDLEELRVRHQSQVEETATLQRRLEESEGELRKSLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSTKRSEDREKGALIEELLQAKQDLQDLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDQEMDKLKEQYDAELQALRESVEEATKNVEVLASRSNSSEQSQAEADLREKVLKEENEKLQGRIAELERRAAQLQRQMEDVKGDEAQAKETLRKCESEVQQLEEALVHARKEEKEATCARRALEKELEQARRELSQVSQEQKELLEKLRDEAEQKEQLRKLKNEMESERWHLDKTIEKLQKEMADIAEASRTSSLELQKQLGEYKEKNRRELAEMQTQLKEKCLEVEKARLAASKMQDELRLKEEELQDYQRAEEEALTKRQLLEQSLKDLEYELEAKSHLKDDRSRLIKQMEDKVSQLEIELEEERTNADLLSERITWSREQMEQMRSELLQEKAAKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLENEERDRANLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMGVNEQLQGQLNSLKKGLRLKTLSSKVLDDSDDDDLSSDAGSLYEAPLSYAFPKDSTIASQI

Target ORF information:

RefSeq Version	NM_026599.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus cingulin-like 1 (Cgnl1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026599.5

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGCGA ATACCAACAC GTACAGCAGG AGTACGGGGT ACACCTGAGA 
CTTGCCAGTG GTGACACTCC AAAGCCAAGG AATTCCCAGC CCTCAAAGGC AGGCTCCTAT 
GGTGTCAGCA TCAGGGTCCA GGGAATTGAT GGCCACCCTT ATATAGTCCT GAATAACACT 
GAGCGGTGTC TAGCAGGCAC ACCTTTCCCA GAAAACGCGC CATCGTTTCC ATCTTCAGTG 
ATAAATAACC TGTCCCTACA TCCAAGCAAT GGAACCGTGT TGAAGGAGAA CACTCCGGAG 
GAACTGCAGC TTCCAGAAAA CCCGTACCTG CAAACCAGCC CGCTAAGAGG CCAGAAGCAG 
TTCTCCCTCC ACGAGGGCAG GAATGGAGTT CTAGAACGCA AAGACGGGCC CACGAAGCTG 
CCCCATGTGC TCAACTTCCA GAGGCACCCG GAGCTTCTGC AGCCCTATGA CCCAGAAAAG 
AATGAGGTGA ACGCAAAGAA GCATCACCCT CCCGAGAGTC CCTGGCTGAG AAATGCAACA 
GAGGACGGGA CCAACTGTAA GAAGTCCCGG AACTGCTTCC CCAAATCCTA CGGTTCCCAG 
CCCAACAGCC CTACTTCGGA AGATTTAGCC AAGACCAACA TGACAGCGAT CCGTCTCTGC 
AGCTCTGTGG TCATAGAAGA CCCCCAAAAG CAGACCTCAG TGTGCGTGAA CGTTCAGAGG 
TGCGCCAAGG AGGGGGTGGG AGAGGAGACC CTTTCCCCTC GACGGAAATC CCCAACTGCC 
CCCAGCCCAC AGGCCTATTC TGAAACCAAG AAAAACAGGC CAGATGTGCT ACCCTTCCGG 
CGACAAGATT CCGCAGGACC CATCCTGGAT GGAGCTCGGT CACGCAGGTC CTCTTCGTCA 
TCCACGACTC CTACTTCTGC CACTTCCTTG TACAAATTTT TACTTGATGA TCAGGAATGC 
GCCATTCATG CCGACAGCGT CAACCGCCAT GAAAACAGAA GGTATATTCC CTTCTTGCCA 
GGAACTGGGC GGGATATCGA CACCTGCTCA ATTCCTGGTG TGGACCAGTT AATTGAAAAA 
TTTGACCAAA AGCCTGGCCT TCAGAGAAGA GGAAGGTCTG GGAAGAGAAA CAGGATCAAT 
CCCGACGACA GGAAAAGATC CCGAAGTGTA GATAGTGCCT TTCCATTTGG TCTCCAGGGA 
AACACAGAAT ACCTCACCGA GTTCAGCAGG AACCTGGGCA AGTCTAGCGA ACACCTCCTT 
CGGCCATCCC AGGTCTTCCC GCAGAGGTCA GTGGCCCAGG AGCACCGTGG GAAGCACAGT 
CCCAGCAGCC CGCCCGCAAA GCTGCAGGGC GCTCAAGGTG CCCACCCCAA GCCTCCCCTG 
CAGAACAAAG ATGGGAAAGT TCTGAACAAA GGAAGGCAGG AAAGCACGGG GGCATGTGCC 
CCCTCCCTGC CAGCACCGAA TAAAAAAGAG GAAGAAATCA AAATAGCCAC TGCCACGTTG 
ATGTTACAGA ACCGGGCGGT GGCTGCCACA TCGGATTCTG GTGCCAAGAA AATTTCAGTG 
AAGACATTTC CTTCCGACTC CAGCACTCAG GCCACACCAG ATCTCTTAAA GGGCCAGCAG 
GAGCTCACAC AACAAACGAA CGAGGAGACC GCCAAGCAGA TCCTTTACAA TTACCTCAAA 
GAAGGAGGCA CAGACAATGA GGATGCCACC AAAAGGAAAG TGAACCTGGT CTTTGAGAAA 
ATCCAGACTT TGAAGTCCAG AGCAGCAGGG AGTGCCCAAG GAAGCAATCA AGCTCCGAAT 
TCTCCCTCTG AAGGCAACAG TCTATTGGAT CAGAAGAACA AATTGATCCT AGAAGTGTCT 
GAACTACAGC AGCAACTGCA ACTGGAGATG AAGAACCAAC AGAACATCAA GGAGGAGCGA 
GAAAGAATGA GAGAGGATTT AGAAGAGCTG CGAGTCCGGC ACCAGAGCCA AGTGGAGGAG 
ACGGCCACCC TGCAGAGACG GCTGGAGGAA AGTGAAGGCG AGCTGAGGAA GAGCTTGGAA 
GAGCTGTTCC AAGTGAAGAT GGAGCGTGAA CAGCACCAGA CTGAGATCAG AGACCTGCAG 
GACCAGCTGT CGGAAATGCA TGATGAGCTA GACAGCACCA AGCGATCCGA GGACAGGGAG 
AAGGGAGCCC TGATTGAGGA GCTCCTGCAA GCCAAGCAGG ACCTTCAAGA TCTGCTCATT 
GCCAAAGAGG AACAGGAAGA TCTCTTGAGG AAGCGGGAGC GGGAGCTGAC TGCCTTGAAG 
GGCGCCTTGA AGGAGGAGGT TTCCAGCCAT GACCAGGAGA TGGATAAGCT GAAGGAACAG 
TACGATGCTG AGCTGCAGGC CCTCAGGGAG AGTGTGGAGG AGGCAACCAA GAATGTTGAG 
GTCCTGGCCA GCAGGAGCAA CTCCTCAGAG CAGAGCCAGG CGGAGGCTGA CCTGCGTGAG 
AAAGTGCTGA AGGAGGAGAA TGAGAAGCTG CAGGGGAGGA TTGCGGAGCT GGAGCGCAGG 
GCAGCCCAGC TACAGAGGCA GATGGAGGAT GTGAAGGGTG ACGAGGCCCA AGCAAAGGAG 
ACACTCCGAA AGTGTGAGAG TGAAGTACAG CAGCTGGAGG AAGCCCTTGT GCATGCCAGA 
AAGGAAGAAA AAGAAGCCAC ATGTGCCAGA AGGGCGCTGG AGAAGGAGCT GGAGCAAGCT 
CGGAGAGAGC TGAGCCAGGT CTCCCAGGAG CAGAAGGAAC TCCTGGAGAA ACTCCGGGAC 
GAGGCGGAGC AGAAGGAGCA GCTGCGAAAG CTGAAGAACG AGATGGAGAG CGAGCGGTGG 
CACCTGGACA AGACCATCGA GAAGCTGCAG AAGGAGATGG CCGACATTGC TGAGGCCTCC 
CGCACATCCT CTTTGGAGCT GCAGAAGCAG CTGGGCGAGT ATAAGGAGAA GAACCGCCGG 
GAGCTTGCGG AAATGCAGAC ACAACTGAAG GAGAAATGCC TGGAAGTGGA AAAGGCCAGA 
CTGGCAGCCT CGAAGATGCA GGATGAGTTA CGCCTCAAGG AGGAAGAGTT GCAGGACTAC 
CAGCGAGCTG AGGAGGAGGC GCTCACAAAA CGGCAGCTTC TGGAGCAGTC GCTGAAGGAC 
CTGGAGTATG AGCTGGAGGC CAAGAGCCAC CTCAAGGATG ACCGCAGCCG ACTCATCAAG 
CAGATGGAGG ACAAGGTGTC CCAATTGGAG ATTGAATTGG AAGAAGAAAG AACCAACGCT 
GACTTGCTGT CGGAGAGGAT CACGTGGAGC AGGGAACAGA TGGAGCAGAT GAGGAGCGAG 
CTTCTTCAGG AGAAAGCTGC CAAGCAAGAC TTAGAGTGTG ACAAGATTTC CCTAGAGAGA 
CAGAACAAAG ACTTAAAGAG CCGGATTATC CACCTGGAAG GTTCCTACAG GTCCAGCAAG 
GAGGGCCTGG TGGTGCAGAT GGAGGCCAGG ATTGCAGAGC TGGAGGACCG GCTGGAGAAT 
GAGGAGAGGG ATCGGGCCAA CCTGCAGCTC AGCAACCGAA GGCTGGAGCG GAAGGTGAAG 
GAGCTGGTGA TGCAGGTGGA TGATGAGCAT CTGTCACTGA CGGACCAGAA GGACCAGCTG 
AGCTTGCGCC TGAAAGCCAT GAAGCGCCAG GTGGAGGAGG CTGAAGAGGA GATCGACCGA 
CTGGAAAGTT CTAAGAAGAA GCTGCAGAGG GAGCTGGAGG AGCAGATGGG GGTGAATGAG 
CAGCTGCAGG GGCAGCTCAA CTCTCTGAAG AAGGGCTTAA GGCTTAAGAC ACTGTCTAGT 
AAAGTACTGG ACGACTCGGA CGACGACGAC CTCAGCAGTG ATGCGGGAAG CCTCTACGAA 
GCCCCACTGA GCTATGCCTT TCCCAAAGAC AGCACTATCG CCAGCCAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cgnl1 ( XM_006511395.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511458.1 Mus musculus cingulin-like 1 (Cgnl1), transcript variant X1, mRNA.

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CloneID	OMu02649
Clone ID Related Accession (Same CDS sequence)	NM_001304362.1 , NM_026599.5 , XM_006511395.1
Accession Version	XM_006511395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cingulin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT ATTTTGGCGA ATACCAACAC GTACAGCAGG AGTACGGGGT ACACCTGAGA 
CTTGCCAGTG GTGACACTCC AAAGCCAAGG AATTCCCAGC CCTCAAAGGC AGGCTCCTAT 
GGTGTCAGCA TCAGGGTCCA GGGAATTGAT GGCCACCCTT ATATAGTCCT GAATAACACT 
GAGCGGTGTC TAGCAGGCAC ACCTTTCCCA GAAAACGCGC CATCGTTTCC ATCTTCAGTG 
ATAAATAACC TGTCCCTACA TCCAAGCAAT GGAACCGTGT TGAAGGAGAA CACTCCGGAG 
GAACTGCAGC TTCCAGAAAA CCCGTACCTG CAAACCAGCC CGCTAAGAGG CCAGAAGCAG 
TTCTCCCTCC ACGAGGGCAG GAATGGAGTT CTAGAACGCA AAGACGGGCC CACGAAGCTG 
CCCCATGTGC TCAACTTCCA GAGGCACCCG GAGCTTCTGC AGCCCTATGA CCCAGAAAAG 
AATGAGGTGA ACGCAAAGAA GCATCACCCT CCCGAGAGTC CCTGGCTGAG AAATGCAACA 
GAGGACGGGA CCAACTGTAA GAAGTCCCGG AACTGCTTCC CCAAATCCTA CGGTTCCCAG 
CCCAACAGCC CTACTTCGGA AGATTTAGCC AAGACCAACA TGACAGCGAT CCGTCTCTGC 
AGCTCTGTGG TCATAGAAGA CCCCCAAAAG CAGACCTCAG TGTGCGTGAA CGTTCAGAGG 
TGCGCCAAGG AGGGGGTGGG AGAGGAGACC CTTTCCCCTC GACGGAAATC CCCAACTGCC 
CCCAGCCCAC AGGCCTATTC TGAAACCAAG AAAAACAGGC CAGATGTGCT ACCCTTCCGG 
CGACAAGATT CCGCAGGACC CATCCTGGAT GGAGCTCGGT CACGCAGGTC CTCTTCGTCA 
TCCACGACTC CTACTTCTGC CACTTCCTTG TACAAATTTT TACTTGATGA TCAGGAATGC 
GCCATTCATG CCGACAGCGT CAACCGCCAT GAAAACAGAA GGTATATTCC CTTCTTGCCA 
GGAACTGGGC GGGATATCGA CACCTGCTCA ATTCCTGGTG TGGACCAGTT AATTGAAAAA 
TTTGACCAAA AGCCTGGCCT TCAGAGAAGA GGAAGGTCTG GGAAGAGAAA CAGGATCAAT 
CCCGACGACA GGAAAAGATC CCGAAGTGTA GATAGTGCCT TTCCATTTGG TCTCCAGGGA 
AACACAGAAT ACCTCACCGA GTTCAGCAGG AACCTGGGCA AGTCTAGCGA ACACCTCCTT 
CGGCCATCCC AGGTCTTCCC GCAGAGGTCA GTGGCCCAGG AGCACCGTGG GAAGCACAGT 
CCCAGCAGCC CGCCCGCAAA GCTGCAGGGC GCTCAAGGTG CCCACCCCAA GCCTCCCCTG 
CAGAACAAAG ATGGGAAAGT TCTGAACAAA GGAAGGCAGG AAAGCACGGG GGCATGTGCC 
CCCTCCCTGC CAGCACCGAA TAAAAAAGAG GAAGAAATCA AAATAGCCAC TGCCACGTTG 
ATGTTACAGA ACCGGGCGGT GGCTGCCACA TCGGATTCTG GTGCCAAGAA AATTTCAGTG 
AAGACATTTC CTTCCGACTC CAGCACTCAG GCCACACCAG ATCTCTTAAA GGGCCAGCAG 
GAGCTCACAC AACAAACGAA CGAGGAGACC GCCAAGCAGA TCCTTTACAA TTACCTCAAA 
GAAGGAGGCA CAGACAATGA GGATGCCACC AAAAGGAAAG TGAACCTGGT CTTTGAGAAA 
ATCCAGACTT TGAAGTCCAG AGCAGCAGGG AGTGCCCAAG GAAGCAATCA AGCTCCGAAT 
TCTCCCTCTG AAGGCAACAG TCTATTGGAT CAGAAGAACA AATTGATCCT AGAAGTGTCT 
GAACTACAGC AGCAACTGCA ACTGGAGATG AAGAACCAAC AGAACATCAA GGAGGAGCGA 
GAAAGAATGA GAGAGGATTT AGAAGAGCTG CGAGTCCGGC ACCAGAGCCA AGTGGAGGAG 
ACGGCCACCC TGCAGAGACG GCTGGAGGAA AGTGAAGGCG AGCTGAGGAA GAGCTTGGAA 
GAGCTGTTCC AAGTGAAGAT GGAGCGTGAA CAGCACCAGA CTGAGATCAG AGACCTGCAG 
GACCAGCTGT CGGAAATGCA TGATGAGCTA GACAGCACCA AGCGATCCGA GGACAGGGAG 
AAGGGAGCCC TGATTGAGGA GCTCCTGCAA GCCAAGCAGG ACCTTCAAGA TCTGCTCATT 
GCCAAAGAGG AACAGGAAGA TCTCTTGAGG AAGCGGGAGC GGGAGCTGAC TGCCTTGAAG 
GGCGCCTTGA AGGAGGAGGT TTCCAGCCAT GACCAGGAGA TGGATAAGCT GAAGGAACAG 
TACGATGCTG AGCTGCAGGC CCTCAGGGAG AGTGTGGAGG AGGCAACCAA GAATGTTGAG 
GTCCTGGCCA GCAGGAGCAA CTCCTCAGAG CAGAGCCAGG CGGAGGCTGA CCTGCGTGAG 
AAAGTGCTGA AGGAGGAGAA TGAGAAGCTG CAGGGGAGGA TTGCGGAGCT GGAGCGCAGG 
GCAGCCCAGC TACAGAGGCA GATGGAGGAT GTGAAGGGTG ACGAGGCCCA AGCAAAGGAG 
ACACTCCGAA AGTGTGAGAG TGAAGTACAG CAGCTGGAGG AAGCCCTTGT GCATGCCAGA 
AAGGAAGAAA AAGAAGCCAC ATGTGCCAGA AGGGCGCTGG AGAAGGAGCT GGAGCAAGCT 
CGGAGAGAGC TGAGCCAGGT CTCCCAGGAG CAGAAGGAAC TCCTGGAGAA ACTCCGGGAC 
GAGGCGGAGC AGAAGGAGCA GCTGCGAAAG CTGAAGAACG AGATGGAGAG CGAGCGGTGG 
CACCTGGACA AGACCATCGA GAAGCTGCAG AAGGAGATGG CCGACATTGC TGAGGCCTCC 
CGCACATCCT CTTTGGAGCT GCAGAAGCAG CTGGGCGAGT ATAAGGAGAA GAACCGCCGG 
GAGCTTGCGG AAATGCAGAC ACAACTGAAG GAGAAATGCC TGGAAGTGGA AAAGGCCAGA 
CTGGCAGCCT CGAAGATGCA GGATGAGTTA CGCCTCAAGG AGGAAGAGTT GCAGGACTAC 
CAGCGAGCTG AGGAGGAGGC GCTCACAAAA CGGCAGCTTC TGGAGCAGTC GCTGAAGGAC 
CTGGAGTATG AGCTGGAGGC CAAGAGCCAC CTCAAGGATG ACCGCAGCCG ACTCATCAAG 
CAGATGGAGG ACAAGGTGTC CCAATTGGAG ATTGAATTGG AAGAAGAAAG AACCAACGCT 
GACTTGCTGT CGGAGAGGAT CACGTGGAGC AGGGAACAGA TGGAGCAGAT GAGGAGCGAG 
CTTCTTCAGG AGAAAGCTGC CAAGCAAGAC TTAGAGTGTG ACAAGATTTC CCTAGAGAGA 
CAGAACAAAG ACTTAAAGAG CCGGATTATC CACCTGGAAG GTTCCTACAG GTCCAGCAAG 
GAGGGCCTGG TGGTGCAGAT GGAGGCCAGG ATTGCAGAGC TGGAGGACCG GCTGGAGAAT 
GAGGAGAGGG ATCGGGCCAA CCTGCAGCTC AGCAACCGAA GGCTGGAGCG GAAGGTGAAG 
GAGCTGGTGA TGCAGGTGGA TGATGAGCAT CTGTCACTGA CGGACCAGAA GGACCAGCTG 
AGCTTGCGCC TGAAAGCCAT GAAGCGCCAG GTGGAGGAGG CTGAAGAGGA GATCGACCGA 
CTGGAAAGTT CTAAGAAGAA GCTGCAGAGG GAGCTGGAGG AGCAGATGGG GGTGAATGAG 
CAGCTGCAGG GGCAGCTCAA CTCTCTGAAG AAGGGCTTAA GGCTTAAGAC ACTGTCTAGT 
AAAGTACTGG ACGACTCGGA CGACGACGAC CTCAGCAGTG ATGCGGGAAG CCTCTACGAA 
GCCCCACTGA GCTATGCCTT TCCCAAAGAC AGCACTATCG CCAGCCAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511458.1
CDS	326..4219
Translation	MELYFGEYQHVQQEYGVHLRLASGDTPKPRNSQPSKAGSYGVSIRVQGIDGHPYIVLNNTERCLAGTPFPENAPSFPSSVINNLSLHPSNGTVLKENTPEELQLPENPYLQTSPLRGQKQFSLHEGRNGVLERKDGPTKLPHVLNFQRHPELLQPYDPEKNEVNAKKHHPPESPWLRNATEDGTNCKKSRNCFPKSYGSQPNSPTSEDLAKTNMTAIRLCSSVVIEDPQKQTSVCVNVQRCAKEGVGEETLSPRRKSPTAPSPQAYSETKKNRPDVLPFRRQDSAGPILDGARSRRSSSSSTTPTSATSLYKFLLDDQECAIHADSVNRHENRRYIPFLPGTGRDIDTCSIPGVDQLIEKFDQKPGLQRRGRSGKRNRINPDDRKRSRSVDSAFPFGLQGNTEYLTEFSRNLGKSSEHLLRPSQVFPQRSVAQEHRGKHSPSSPPAKLQGAQGAHPKPPLQNKDGKVLNKGRQESTGACAPSLPAPNKKEEEIKIATATLMLQNRAVAATSDSGAKKISVKTFPSDSSTQATPDLLKGQQELTQQTNEETAKQILYNYLKEGGTDNEDATKRKVNLVFEKIQTLKSRAAGSAQGSNQAPNSPSEGNSLLDQKNKLILEVSELQQQLQLEMKNQQNIKEERERMREDLEELRVRHQSQVEETATLQRRLEESEGELRKSLEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSTKRSEDREKGALIEELLQAKQDLQDLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDQEMDKLKEQYDAELQALRESVEEATKNVEVLASRSNSSEQSQAEADLREKVLKEENEKLQGRIAELERRAAQLQRQMEDVKGDEAQAKETLRKCESEVQQLEEALVHARKEEKEATCARRALEKELEQARRELSQVSQEQKELLEKLRDEAEQKEQLRKLKNEMESERWHLDKTIEKLQKEMADIAEASRTSSLELQKQLGEYKEKNRRELAEMQTQLKEKCLEVEKARLAASKMQDELRLKEEELQDYQRAEEEALTKRQLLEQSLKDLEYELEAKSHLKDDRSRLIKQMEDKVSQLEIELEEERTNADLLSERITWSREQMEQMRSELLQEKAAKQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEGLVVQMEARIAELEDRLENEERDRANLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQLSLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMGVNEQLQGQLNSLKKGLRLKTLSSKVLDDSDDDDLSSDAGSLYEAPLSYAFPKDSTIASQI

Target ORF information:

RefSeq Version	XM_006511395.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cingulin-like 1 (Cgnl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511395.1

ORF Insert Sequence:

ATGGAGCTGT ATTTTGGCGA ATACCAACAC GTACAGCAGG AGTACGGGGT ACACCTGAGA 
CTTGCCAGTG GTGACACTCC AAAGCCAAGG AATTCCCAGC CCTCAAAGGC AGGCTCCTAT 
GGTGTCAGCA TCAGGGTCCA GGGAATTGAT GGCCACCCTT ATATAGTCCT GAATAACACT 
GAGCGGTGTC TAGCAGGCAC ACCTTTCCCA GAAAACGCGC CATCGTTTCC ATCTTCAGTG 
ATAAATAACC TGTCCCTACA TCCAAGCAAT GGAACCGTGT TGAAGGAGAA CACTCCGGAG 
GAACTGCAGC TTCCAGAAAA CCCGTACCTG CAAACCAGCC CGCTAAGAGG CCAGAAGCAG 
TTCTCCCTCC ACGAGGGCAG GAATGGAGTT CTAGAACGCA AAGACGGGCC CACGAAGCTG 
CCCCATGTGC TCAACTTCCA GAGGCACCCG GAGCTTCTGC AGCCCTATGA CCCAGAAAAG 
AATGAGGTGA ACGCAAAGAA GCATCACCCT CCCGAGAGTC CCTGGCTGAG AAATGCAACA 
GAGGACGGGA CCAACTGTAA GAAGTCCCGG AACTGCTTCC CCAAATCCTA CGGTTCCCAG 
CCCAACAGCC CTACTTCGGA AGATTTAGCC AAGACCAACA TGACAGCGAT CCGTCTCTGC 
AGCTCTGTGG TCATAGAAGA CCCCCAAAAG CAGACCTCAG TGTGCGTGAA CGTTCAGAGG 
TGCGCCAAGG AGGGGGTGGG AGAGGAGACC CTTTCCCCTC GACGGAAATC CCCAACTGCC 
CCCAGCCCAC AGGCCTATTC TGAAACCAAG AAAAACAGGC CAGATGTGCT ACCCTTCCGG 
CGACAAGATT CCGCAGGACC CATCCTGGAT GGAGCTCGGT CACGCAGGTC CTCTTCGTCA 
TCCACGACTC CTACTTCTGC CACTTCCTTG TACAAATTTT TACTTGATGA TCAGGAATGC 
GCCATTCATG CCGACAGCGT CAACCGCCAT GAAAACAGAA GGTATATTCC CTTCTTGCCA 
GGAACTGGGC GGGATATCGA CACCTGCTCA ATTCCTGGTG TGGACCAGTT AATTGAAAAA 
TTTGACCAAA AGCCTGGCCT TCAGAGAAGA GGAAGGTCTG GGAAGAGAAA CAGGATCAAT 
CCCGACGACA GGAAAAGATC CCGAAGTGTA GATAGTGCCT TTCCATTTGG TCTCCAGGGA 
AACACAGAAT ACCTCACCGA GTTCAGCAGG AACCTGGGCA AGTCTAGCGA ACACCTCCTT 
CGGCCATCCC AGGTCTTCCC GCAGAGGTCA GTGGCCCAGG AGCACCGTGG GAAGCACAGT 
CCCAGCAGCC CGCCCGCAAA GCTGCAGGGC GCTCAAGGTG CCCACCCCAA GCCTCCCCTG 
CAGAACAAAG ATGGGAAAGT TCTGAACAAA GGAAGGCAGG AAAGCACGGG GGCATGTGCC 
CCCTCCCTGC CAGCACCGAA TAAAAAAGAG GAAGAAATCA AAATAGCCAC TGCCACGTTG 
ATGTTACAGA ACCGGGCGGT GGCTGCCACA TCGGATTCTG GTGCCAAGAA AATTTCAGTG 
AAGACATTTC CTTCCGACTC CAGCACTCAG GCCACACCAG ATCTCTTAAA GGGCCAGCAG 
GAGCTCACAC AACAAACGAA CGAGGAGACC GCCAAGCAGA TCCTTTACAA TTACCTCAAA 
GAAGGAGGCA CAGACAATGA GGATGCCACC AAAAGGAAAG TGAACCTGGT CTTTGAGAAA 
ATCCAGACTT TGAAGTCCAG AGCAGCAGGG AGTGCCCAAG GAAGCAATCA AGCTCCGAAT 
TCTCCCTCTG AAGGCAACAG TCTATTGGAT CAGAAGAACA AATTGATCCT AGAAGTGTCT 
GAACTACAGC AGCAACTGCA ACTGGAGATG AAGAACCAAC AGAACATCAA GGAGGAGCGA 
GAAAGAATGA GAGAGGATTT AGAAGAGCTG CGAGTCCGGC ACCAGAGCCA AGTGGAGGAG 
ACGGCCACCC TGCAGAGACG GCTGGAGGAA AGTGAAGGCG AGCTGAGGAA GAGCTTGGAA 
GAGCTGTTCC AAGTGAAGAT GGAGCGTGAA CAGCACCAGA CTGAGATCAG AGACCTGCAG 
GACCAGCTGT CGGAAATGCA TGATGAGCTA GACAGCACCA AGCGATCCGA GGACAGGGAG 
AAGGGAGCCC TGATTGAGGA GCTCCTGCAA GCCAAGCAGG ACCTTCAAGA TCTGCTCATT 
GCCAAAGAGG AACAGGAAGA TCTCTTGAGG AAGCGGGAGC GGGAGCTGAC TGCCTTGAAG 
GGCGCCTTGA AGGAGGAGGT TTCCAGCCAT GACCAGGAGA TGGATAAGCT GAAGGAACAG 
TACGATGCTG AGCTGCAGGC CCTCAGGGAG AGTGTGGAGG AGGCAACCAA GAATGTTGAG 
GTCCTGGCCA GCAGGAGCAA CTCCTCAGAG CAGAGCCAGG CGGAGGCTGA CCTGCGTGAG 
AAAGTGCTGA AGGAGGAGAA TGAGAAGCTG CAGGGGAGGA TTGCGGAGCT GGAGCGCAGG 
GCAGCCCAGC TACAGAGGCA GATGGAGGAT GTGAAGGGTG ACGAGGCCCA AGCAAAGGAG 
ACACTCCGAA AGTGTGAGAG TGAAGTACAG CAGCTGGAGG AAGCCCTTGT GCATGCCAGA 
AAGGAAGAAA AAGAAGCCAC ATGTGCCAGA AGGGCGCTGG AGAAGGAGCT GGAGCAAGCT 
CGGAGAGAGC TGAGCCAGGT CTCCCAGGAG CAGAAGGAAC TCCTGGAGAA ACTCCGGGAC 
GAGGCGGAGC AGAAGGAGCA GCTGCGAAAG CTGAAGAACG AGATGGAGAG CGAGCGGTGG 
CACCTGGACA AGACCATCGA GAAGCTGCAG AAGGAGATGG CCGACATTGC TGAGGCCTCC 
CGCACATCCT CTTTGGAGCT GCAGAAGCAG CTGGGCGAGT ATAAGGAGAA GAACCGCCGG 
GAGCTTGCGG AAATGCAGAC ACAACTGAAG GAGAAATGCC TGGAAGTGGA AAAGGCCAGA 
CTGGCAGCCT CGAAGATGCA GGATGAGTTA CGCCTCAAGG AGGAAGAGTT GCAGGACTAC 
CAGCGAGCTG AGGAGGAGGC GCTCACAAAA CGGCAGCTTC TGGAGCAGTC GCTGAAGGAC 
CTGGAGTATG AGCTGGAGGC CAAGAGCCAC CTCAAGGATG ACCGCAGCCG ACTCATCAAG 
CAGATGGAGG ACAAGGTGTC CCAATTGGAG ATTGAATTGG AAGAAGAAAG AACCAACGCT 
GACTTGCTGT CGGAGAGGAT CACGTGGAGC AGGGAACAGA TGGAGCAGAT GAGGAGCGAG 
CTTCTTCAGG AGAAAGCTGC CAAGCAAGAC TTAGAGTGTG ACAAGATTTC CCTAGAGAGA 
CAGAACAAAG ACTTAAAGAG CCGGATTATC CACCTGGAAG GTTCCTACAG GTCCAGCAAG 
GAGGGCCTGG TGGTGCAGAT GGAGGCCAGG ATTGCAGAGC TGGAGGACCG GCTGGAGAAT 
GAGGAGAGGG ATCGGGCCAA CCTGCAGCTC AGCAACCGAA GGCTGGAGCG GAAGGTGAAG 
GAGCTGGTGA TGCAGGTGGA TGATGAGCAT CTGTCACTGA CGGACCAGAA GGACCAGCTG 
AGCTTGCGCC TGAAAGCCAT GAAGCGCCAG GTGGAGGAGG CTGAAGAGGA GATCGACCGA 
CTGGAAAGTT CTAAGAAGAA GCTGCAGAGG GAGCTGGAGG AGCAGATGGG GGTGAATGAG 
CAGCTGCAGG GGCAGCTCAA CTCTCTGAAG AAGGGCTTAA GGCTTAAGAC ACTGTCTAGT 
AAAGTACTGG ACGACTCGGA CGACGACGAC CTCAGCAGTG ATGCGGGAAG CCTCTACGAA 
GCCCCACTGA GCTATGCCTT TCCCAAAGAC AGCACTATCG CCAGCCAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cgnl1, an endothelial junction complex protein, regulates GTPase mediated angiogenesis.
Cardiovascular research113(14)1776-1788(2017 Dec)
Chrifi I,Hermkens D,Brandt MM,van Dijk CGM,B?rgisser PE,Haasdijk R,Pei J,van de Kamp EHM,Zhu C,Blonden L,Kros JM,Duncker DJ,Duckers HJ,Cheng C

Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
Journal of cell science125(Pt 21)5005-14(2012 Nov)
Guillemot L,Schneider Y,Brun P,Castagliuolo I,Pizzuti D,Martines D,Jond L,Bongiovanni M,Citi S

Cingulin, paracingulin, and PLEKHA7: signaling and cytoskeletal adaptors at the apical junctional complex.
Annals of the New York Academy of Sciences1257125-32(2012 Jun)
Citi S,Pulimeno P,Paschoud S

Endocardiogenesis in embryoid bodies: novel markers identified by gene expression profiling.
Biochemical and biophysical research communications357(4)896-902(2007 Jun)
Narumiya H,Hidaka K,Shirai M,Terami H,Aburatani H,Morisaki T

JACOP, a novel plaque protein localizing at the apical junctional complex with sequence similarity to cingulin.
The Journal of biological chemistry279(44)46014-22(2004 Oct)
Ohnishi H,Nakahara T,Furuse K,Sasaki H,Tsukita S,Furuse M

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Cgnl1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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