Ufl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ufl1
Entrez Gene ID	67490
Full Name	UFM1 specific ligase 1
Synonyms	1810074P20Rik,AI429228,Kiaa0776,Maxer,Rcad,mKIAA0776
General protein information	Preferred Names UFM1 specific ligase 1 Names E3 UFM1-protein ligase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538204.3	XP_006538267.1	E3 UFM1-protein ligase 1 isoform X2
NM_001355512.1	NP_001342441.1	E3 UFM1-protein ligase 1
XM_006538203.3	XP_006538266.1	E3 UFM1-protein ligase 1 isoform X1
NM_026194.4	NP_080470.2	E3 UFM1-protein ligase 1
NM_026194.5	NP_080470.2	E3 UFM1-protein ligase 1

Pathways from BioSystems

Homologies

	UFL1	XP_001100312.1
Canis lupus familiaris (dog)	UFL1	XP_532236.4
Bos taurus (cattle)	UFL1	NP_001073745.1
Anopheles gambiae (African malaria mosquito)	UFL1_ANOGA	XP_317778.3
Arabidopsis thaliana (thale cress)	AT3G46220	NP_566883.4
Homo sapiens (human)	UFL1	NP_056138.1
Gallus gallus (chicken)	UFL1	NP_001096671.1
Mus musculus (house mouse)	Ufl1	NP_080470.2
Rattus norvegicus (Norway rat)	Ufl1	NP_001119751.1
Drosophila melanogaster (fruit fly)	CG1104	NP_001246957.1
Oryza sativa (rice)	Os05g0117600	NP_001174184.1
Xenopus tropicalis (tropical clawed frog)	ufl1	XP_002934919.2
Pan troglodytes (chimpanzee)	UFL1	XP_518643.2
Danio rerio (zebrafish)	ufl1	NP_956900.1

Gene Ontology
Bibliography

Related articles in PubMed

RCAD/BiP pathway is necessary for the proper synthesis of digestive enzymes and secretory function of the exocrine pancreas.
Miller C, Cai Y, Patton T, Graves SH, Li H, Sabbatini ME
American journal of physiology. Gastrointestinal and liver physiology312(3)G314-G326(2017 Mar)

RCAD/Ufl1, a Ufm1 E3 ligase, is essential for hematopoietic stem cell function and murine hematopoiesis.
Zhang M, Zhu X, Zhang Y, Cai Y, Chen J, Sivaprakasam S, Gurav A, Pi W, Makala L, Wu J, Pace B, Tuan-Lo D, Ganapathy V, Singh N, Li H
Cell death and differentiation22(12)1922-34(2015 Dec)

Transcriptional regulation of the Ufm1 conjugation system in response to disturbance of the endoplasmic reticulum homeostasis and inhibition of vesicle trafficking.
Zhang Y, Zhang M, Wu J, Lei G, Li H
PloS one7(11)e48587(2012)

Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis.
Lemaire K, Moura RF, Granvik M, Igoillo-Esteve M, Hohmeier HE, Hendrickx N, Newgard CB, Waelkens E, Cnop M, Schuit F
PloS one6(4)e18517(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RCAD/BiP pathway is required for the proper synthesis and secretion of amylase from pancreatic acini, as well as for the maintenance of the ER homeostasis.
Title: RCAD/BiP pathway is necessary for the proper synthesis of digestive enzymes and secretory function of the exocrine pancreas.

Results identify a novel molecule named multiple alpha-helix protein located at ER (Maxer) downregulated by mutant ataxin-1 (Atx1) in Bergmann glia.
Title: Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity.

The following Ufl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ufl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33107	XM_006538204.3 Latest version!	Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15746	NM_001355512.1 Latest version!	Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu15746	NM_026194.5 Latest version!	Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu15746	XM_006538203.3 Latest version!	Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OMu15746	NM_026194.4	Mus musculus UFM1 specific ligase 1 (Ufl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ufl1 ( XM_006538204.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538267.1 Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu33107
Clone ID Related Accession (Same CDS sequence)	XM_006538204.3
Accession Version	XM_006538204.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 UFM1-protein ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538204.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC AGCAGGTGAT GAGACCATTC GGCAAGCTCG CTTCAGCTAT AGTCTTTAGT 
GACACGGTTG TGGTGAGTGA AAAGTTCATA ACTGACTGCA CAGGACTGTT CAGCGAACGG 
ATGCACCAAA AAGCTGAGAA GGAAATGAAA AATAATCCTG TGCATTTAAT CACTGAAGAA 
GATCTGAAAC AAATTTCCAT TTTAGAAAGT GTTAATACAA GTAAAAAGGA TAAAAAAGAT 
GAACGCAGGA AGAAAGCAAC AGAGGGCTCT GGAAGTGTAA GAGGAGGAGG AGGGGGCAAT 
GCCAGAGAAT ATAAAATTAA GAAAACCAAG AAGAAGGGAA GAAAAGATGA GGACAGTGAT 
GATGAGTCCC AGTCGTCGCA TGGTGGAAAG AAGAAGCCGG ACATCACATT CATGTTCCAG 
GATGAAATTG AAGATTGCTT AAGGAAACAC ATTCAGGATG CCCCTGAGGA GTTTATTTCA 
GAGCTTGCAG AATACTTAAT AAAGCCTCTT AATAAAATGT ATCTTGAGGT AGTACGTTCA 
GTCTTCATGT CTTCAACATC TGCTTCTGGA ACTGGCAGAA AACGCACAAT CAAGGACTTA 
CAAGAAGAGG TTTCAAACCT ATACAATAAT ATCAGATTAT TTGAAAAGGG AATGAAATAT 
TTTGCAGATG ACACACAGAC TGCTCTTACC AAGCACTTGC TGAAGACAGT GTGTACCGAT 
ATCACTAACC TCATGTTCAA CTTCCTAGCC TCAGATTTCC TCATGGCAGT GGAGGAGCCT 
GCAGCCATTA CAAGTGATAT AAGAAAAAAA ATTTTAAGCA AATTGACAGA AGAAACCAAA 
GTAGCTCTCA CAAAACTCCA TAACTCTCTC AATGAAAAAA GCATAGAAGA CTTCCTGTCT 
TGCCTGGATT CTGCCACAGA AGCGTGTGAC ATTATGGTGA AAAAGGGAGA CAAGAAAAGG 
GAAAGACAAA TCCTCTTCCA GCATCGACAG GCTCTGTGTG AGCAGCTGAA GGTCACAGAA 
GACCCTGCTC TGATTTTACA CCTCACAGCA GTCCTGTTAT TTCAGCTCTC AACCCACAGC 
ATGCTCCACG CACCTGGAAG GTGTGTCCCA CAGATCATCG CGTTTCTTCA CAGCAAAATC 
CCAGAGGATC AACATACTCT CTTGGTAAAA TATCAAGGTT TGGTTGTGAA ACAGTTAGTC 
AGTCAAAATA AGAAGACTGG GCAGGGAGAG GATCCGTCAA GCGATGAATT AGACAAGGAA 
CAACATGATG TCACAAACGC TACTCGGAAA GAGCTTCAGG AACTGTCATT ATCCATTAAG 
GACCTTGTTC TCAAGTCCAG GAAATCATCT GTCACAGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538267.1
CDS	642..2006
Translation	MLLQQVMRPFGKLASAIVFSDTVVVSEKFITDCTGLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTSKKDKKDERRKKATEGSGSVRGGGGGNAREYKIKKTKKKGRKDEDSDDESQSSHGGKKKPDITFMFQDEIEDCLRKHIQDAPEEFISELAEYLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTALTKHLLKTVCTDITNLMFNFLASDFLMAVEEPAAITSDIRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCEQLKVTEDPALILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVSQNKKTGQGEDPSSDELDKEQHDVTNATRKELQELSLSIKDLVLKSRKSSVTEE

Target ORF information:

RefSeq Version	XM_006538204.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538204.3

ORF Insert Sequence:

ATGCTGCTCC AGCAGGTGAT GAGACCATTC GGCAAGCTCG CTTCAGCTAT AGTCTTTAGT 
GACACGGTTG TGGTGAGTGA AAAGTTCATA ACTGACTGCA CAGGACTGTT CAGCGAACGG 
ATGCACCAAA AAGCTGAGAA GGAAATGAAA AATAATCCTG TGCATTTAAT CACTGAAGAA 
GATCTGAAAC AAATTTCCAT TTTAGAAAGT GTTAATACAA GTAAAAAGGA TAAAAAAGAT 
GAACGCAGGA AGAAAGCAAC AGAGGGCTCT GGAAGTGTAA GAGGAGGAGG AGGGGGCAAT 
GCCAGAGAAT ATAAAATTAA GAAAACCAAG AAGAAGGGAA GAAAAGATGA GGACAGTGAT 
GATGAGTCCC AGTCGTCGCA TGGTGGAAAG AAGAAGCCGG ACATCACATT CATGTTCCAG 
GATGAAATTG AAGATTGCTT AAGGAAACAC ATTCAGGATG CCCCTGAGGA GTTTATTTCA 
GAGCTTGCAG AATACTTAAT AAAGCCTCTT AATAAAATGT ATCTTGAGGT AGTACGTTCA 
GTCTTCATGT CTTCAACATC TGCTTCTGGA ACTGGCAGAA AACGCACAAT CAAGGACTTA 
CAAGAAGAGG TTTCAAACCT ATACAATAAT ATCAGATTAT TTGAAAAGGG AATGAAATAT 
TTTGCAGATG ACACACAGAC TGCTCTTACC AAGCACTTGC TGAAGACAGT GTGTACCGAT 
ATCACTAACC TCATGTTCAA CTTCCTAGCC TCAGATTTCC TCATGGCAGT GGAGGAGCCT 
GCAGCCATTA CAAGTGATAT AAGAAAAAAA ATTTTAAGCA AATTGACAGA AGAAACCAAA 
GTAGCTCTCA CAAAACTCCA TAACTCTCTC AATGAAAAAA GCATAGAAGA CTTCCTGTCT 
TGCCTGGATT CTGCCACAGA AGCGTGTGAC ATTATGGTGA AAAAGGGAGA CAAGAAAAGG 
GAAAGACAAA TCCTCTTCCA GCATCGACAG GCTCTGTGTG AGCAGCTGAA GGTCACAGAA 
GACCCTGCTC TGATTTTACA CCTCACAGCA GTCCTGTTAT TTCAGCTCTC AACCCACAGC 
ATGCTCCACG CACCTGGAAG GTGTGTCCCA CAGATCATCG CGTTTCTTCA CAGCAAAATC 
CCAGAGGATC AACATACTCT CTTGGTAAAA TATCAAGGTT TGGTTGTGAA ACAGTTAGTC 
AGTCAAAATA AGAAGACTGG GCAGGGAGAG GATCCGTCAA GCGATGAATT AGACAAGGAA 
CAACATGATG TCACAAACGC TACTCGGAAA GAGCTTCAGG AACTGTCATT ATCCATTAAG 
GACCTTGTTC TCAAGTCCAG GAAATCATCT GTCACAGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ufl1 ( NM_001355512.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342441.1 Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15746
Clone ID Related Accession (Same CDS sequence)	NM_001355512.1 , XM_006538203.3 , NM_026194.4 , NM_026194.5
Accession Version	NM_001355512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	E3 UFM1-protein ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY097965.1, AK132697.1, AL365061.1, AL935267.13 and BM900014.1. On Sep 19, 2017 this sequence version replaced XM_006538203.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.250863.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG CCTGGGAGGA GATCCGGCGG CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCAGAGTCCA CGCAAAGGTT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTCCATACA CTTGACGGCA AGGAATATAT CACTCCAGCC 
CAAATCAGTA AAGAAATGAG AGACGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTT 
GACTTACAAC AGGTTATTAA TGTGGACCTG ACTCATATCG AGAGTAGAGT CAGTGATATT 
ATTAAATCAG AGAAGCACGT GCAGATGGTA CTGGGACAAC TGATTGATGA GAACTATCTG 
GATCAGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACTGTCTCA 
GAACTGTGTA AAGCCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTGGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTAG CTCGCCATAA AGCACGTATC CGCGGACTCT TCAGTGCCAT TACACGGCCT 
ACACCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTCGAGGACC TCGTCAGCAC TGGCCGCTTA CGAGGCACCG TGGTCGGCGG GCGACAGGAT 
AAAGCAGTCT TTGTCCCTGA CATCTACTCC AGGACACAGA GTACTTGGGT GGACTCCTTT 
TTCAGGCAGA ATGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCAACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GACTCGTAGA CCAGGTGGAA GCATCCGTAG AGGAAGCCAT CAGCTCAGGG 
ACCTGGGTTG ACATTTCGCC TCTGCTACCC AGTTCCTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTCCAGC AGGTGATGAG ACCATTCGGC AAGCTCGCTT CAGCTATAGT CTTTAGTGAC 
ACGGTTGTGG TGAGTGAAAA GTTCATAACT GACTGCACAG GACTGTTCAG CGAACGGATG 
CACCAAAAAG CTGAGAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC TGAAGAAGAT 
CTGAAACAAA TTTCCATTTT AGAAAGTGTT AATACAAGTA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGCTCTGGA AGTGTAAGAG GAGGAGGAGG GGGCAATGCC 
AGAGAATATA AAATTAAGAA AACCAAGAAG AAGGGAAGAA AAGATGAGGA CAGTGATGAT 
GAGTCCCAGT CGTCGCATGG TGGAAAGAAG AAGCCGGACA TCACATTCAT GTTCCAGGAT 
GAAATTGAAG ATTGCTTAAG GAAACACATT CAGGATGCCC CTGAGGAGTT TATTTCAGAG 
CTTGCAGAAT ACTTAATAAA GCCTCTTAAT AAAATGTATC TTGAGGTAGT ACGTTCAGTC 
TTCATGTCTT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACAATCAA GGACTTACAA 
GAAGAGGTTT CAAACCTATA CAATAATATC AGATTATTTG AAAAGGGAAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTTACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GATTTCCTCA TGGCAGTGGA GGAGCCTGCA 
GCCATTACAA GTGATATAAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACCAAAGTA 
GCTCTCACAA AACTCCATAA CTCTCTCAAT GAAAAAAGCA TAGAAGACTT CCTGTCTTGC 
CTGGATTCTG CCACAGAAGC GTGTGACATT ATGGTGAAAA AGGGAGACAA GAAAAGGGAA 
AGACAAATCC TCTTCCAGCA TCGACAGGCT CTGTGTGAGC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACACCT CACAGCAGTC CTGTTATTTC AGCTCTCAAC CCACAGCATG 
CTCCACGCAC CTGGAAGGTG TGTCCCACAG ATCATCGCGT TTCTTCACAG CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGTTTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGACTGGGCA GGGAGAGGAT CCGTCAAGCG ATGAATTAGA CAAGGAACAA 
CATGATGTCA CAAACGCTAC TCGGAAAGAG CTTCAGGAAC TGTCATTATC CATTAAGGAC 
CTTGTTCTCA AGTCCAGGAA ATCATCTGTC ACAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342441.1
CDS	70..2451
Translation	MADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKYGFQEQLLYSVLEDLVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTQLLFLKATCVGQGLVDQVEASVEEAISSGTWVDISPLLPSSLSVEDAAMLLQQVMRPFGKLASAIVFSDTVVVSEKFITDCTGLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTSKKDKKDERRKKATEGSGSVRGGGGGNAREYKIKKTKKKGRKDEDSDDESQSSHGGKKKPDITFMFQDEIEDCLRKHIQDAPEEFISELAEYLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTALTKHLLKTVCTDITNLMFNFLASDFLMAVEEPAAITSDIRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCEQLKVTEDPALILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVSQNKKTGQGEDPSSDELDKEQHDVTNATRKELQELSLSIKDLVLKSRKSSVTEE

Target ORF information:

RefSeq Version	NM_001355512.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355512.1

ORF Insert Sequence:

ATGGCGGACG CCTGGGAGGA GATCCGGCGG CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCAGAGTCCA CGCAAAGGTT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTCCATACA CTTGACGGCA AGGAATATAT CACTCCAGCC 
CAAATCAGTA AAGAAATGAG AGACGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTT 
GACTTACAAC AGGTTATTAA TGTGGACCTG ACTCATATCG AGAGTAGAGT CAGTGATATT 
ATTAAATCAG AGAAGCACGT GCAGATGGTA CTGGGACAAC TGATTGATGA GAACTATCTG 
GATCAGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACTGTCTCA 
GAACTGTGTA AAGCCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTGGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTAG CTCGCCATAA AGCACGTATC CGCGGACTCT TCAGTGCCAT TACACGGCCT 
ACACCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTCGAGGACC TCGTCAGCAC TGGCCGCTTA CGAGGCACCG TGGTCGGCGG GCGACAGGAT 
AAAGCAGTCT TTGTCCCTGA CATCTACTCC AGGACACAGA GTACTTGGGT GGACTCCTTT 
TTCAGGCAGA ATGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCAACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GACTCGTAGA CCAGGTGGAA GCATCCGTAG AGGAAGCCAT CAGCTCAGGG 
ACCTGGGTTG ACATTTCGCC TCTGCTACCC AGTTCCTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTCCAGC AGGTGATGAG ACCATTCGGC AAGCTCGCTT CAGCTATAGT CTTTAGTGAC 
ACGGTTGTGG TGAGTGAAAA GTTCATAACT GACTGCACAG GACTGTTCAG CGAACGGATG 
CACCAAAAAG CTGAGAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC TGAAGAAGAT 
CTGAAACAAA TTTCCATTTT AGAAAGTGTT AATACAAGTA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGCTCTGGA AGTGTAAGAG GAGGAGGAGG GGGCAATGCC 
AGAGAATATA AAATTAAGAA AACCAAGAAG AAGGGAAGAA AAGATGAGGA CAGTGATGAT 
GAGTCCCAGT CGTCGCATGG TGGAAAGAAG AAGCCGGACA TCACATTCAT GTTCCAGGAT 
GAAATTGAAG ATTGCTTAAG GAAACACATT CAGGATGCCC CTGAGGAGTT TATTTCAGAG 
CTTGCAGAAT ACTTAATAAA GCCTCTTAAT AAAATGTATC TTGAGGTAGT ACGTTCAGTC 
TTCATGTCTT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACAATCAA GGACTTACAA 
GAAGAGGTTT CAAACCTATA CAATAATATC AGATTATTTG AAAAGGGAAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTTACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GATTTCCTCA TGGCAGTGGA GGAGCCTGCA 
GCCATTACAA GTGATATAAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACCAAAGTA 
GCTCTCACAA AACTCCATAA CTCTCTCAAT GAAAAAAGCA TAGAAGACTT CCTGTCTTGC 
CTGGATTCTG CCACAGAAGC GTGTGACATT ATGGTGAAAA AGGGAGACAA GAAAAGGGAA 
AGACAAATCC TCTTCCAGCA TCGACAGGCT CTGTGTGAGC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACACCT CACAGCAGTC CTGTTATTTC AGCTCTCAAC CCACAGCATG 
CTCCACGCAC CTGGAAGGTG TGTCCCACAG ATCATCGCGT TTCTTCACAG CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGTTTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGACTGGGCA GGGAGAGGAT CCGTCAAGCG ATGAATTAGA CAAGGAACAA 
CATGATGTCA CAAACGCTAC TCGGAAAGAG CTTCAGGAAC TGTCATTATC CATTAAGGAC 
CTTGTTCTCA AGTCCAGGAA ATCATCTGTC ACAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ufl1 ( NM_026194.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080470.2 Mus musculus UFM1 specific ligase 1 (Ufl1), mRNA.

Price & Availability↑

CloneID	OMu15746
Clone ID Related Accession (Same CDS sequence)	NM_001355512.1 , XM_006538203.3 , NM_026194.4 , NM_026194.5
Accession Version	NM_026194.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-05
Organism	Mus musculus(House mouse)
Product	E3 UFM1-protein ligase 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY097965.1, AK132697.1, BY368866.1 and AV274915.1. On Apr 22, 2009 this sequence version replaced NM_026194.3. ##Evidence-Data-START## Transcript exon combination :: AK132697.1, AK007993.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACG CCTGGGAGGA GATCCGGCGG CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCAGAGTCCA CGCAAAGGTT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTCCATACA CTTGACGGCA AGGAATATAT CACTCCAGCC 
CAAATCAGTA AAGAAATGAG AGACGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTT 
GACTTACAAC AGGTTATTAA TGTGGACCTG ACTCATATCG AGAGTAGAGT CAGTGATATT 
ATTAAATCAG AGAAGCACGT GCAGATGGTA CTGGGACAAC TGATTGATGA GAACTATCTG 
GATCAGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACTGTCTCA 
GAACTGTGTA AAGCCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTGGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTAG CTCGCCATAA AGCACGTATC CGCGGACTCT TCAGTGCCAT TACACGGCCT 
ACACCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTCGAGGACC TCGTCAGCAC TGGCCGCTTA CGAGGCACCG TGGTCGGCGG GCGACAGGAT 
AAAGCAGTCT TTGTCCCTGA CATCTACTCC AGGACACAGA GTACTTGGGT GGACTCCTTT 
TTCAGGCAGA ATGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCAACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GACTCGTAGA CCAGGTGGAA GCATCCGTAG AGGAAGCCAT CAGCTCAGGG 
ACCTGGGTTG ACATTTCGCC TCTGCTACCC AGTTCCTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTCCAGC AGGTGATGAG ACCATTCGGC AAGCTCGCTT CAGCTATAGT CTTTAGTGAC 
ACGGTTGTGG TGAGTGAAAA GTTCATAACT GACTGCACAG GACTGTTCAG CGAACGGATG 
CACCAAAAAG CTGAGAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC TGAAGAAGAT 
CTGAAACAAA TTTCCATTTT AGAAAGTGTT AATACAAGTA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGCTCTGGA AGTGTAAGAG GAGGAGGAGG GGGCAATGCC 
AGAGAATATA AAATTAAGAA AACCAAGAAG AAGGGAAGAA AAGATGAGGA CAGTGATGAT 
GAGTCCCAGT CGTCGCATGG TGGAAAGAAG AAGCCGGACA TCACATTCAT GTTCCAGGAT 
GAAATTGAAG ATTGCTTAAG GAAACACATT CAGGATGCCC CTGAGGAGTT TATTTCAGAG 
CTTGCAGAAT ACTTAATAAA GCCTCTTAAT AAAATGTATC TTGAGGTAGT ACGTTCAGTC 
TTCATGTCTT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACAATCAA GGACTTACAA 
GAAGAGGTTT CAAACCTATA CAATAATATC AGATTATTTG AAAAGGGAAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTTACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GATTTCCTCA TGGCAGTGGA GGAGCCTGCA 
GCCATTACAA GTGATATAAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACCAAAGTA 
GCTCTCACAA AACTCCATAA CTCTCTCAAT GAAAAAAGCA TAGAAGACTT CCTGTCTTGC 
CTGGATTCTG CCACAGAAGC GTGTGACATT ATGGTGAAAA AGGGAGACAA GAAAAGGGAA 
AGACAAATCC TCTTCCAGCA TCGACAGGCT CTGTGTGAGC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACACCT CACAGCAGTC CTGTTATTTC AGCTCTCAAC CCACAGCATG 
CTCCACGCAC CTGGAAGGTG TGTCCCACAG ATCATCGCGT TTCTTCACAG CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGTTTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGACTGGGCA GGGAGAGGAT CCGTCAAGCG ATGAATTAGA CAAGGAACAA 
CATGATGTCA CAAACGCTAC TCGGAAAGAG CTTCAGGAAC TGTCATTATC CATTAAGGAC 
CTTGTTCTCA AGTCCAGGAA ATCATCTGTC ACAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080470.2
CDS	70..2451
Translation	MADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKYGFQEQLLYSVLEDLVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTQLLFLKATCVGQGLVDQVEASVEEAISSGTWVDISPLLPSSLSVEDAAMLLQQVMRPFGKLASAIVFSDTVVVSEKFITDCTGLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTSKKDKKDERRKKATEGSGSVRGGGGGNAREYKIKKTKKKGRKDEDSDDESQSSHGGKKKPDITFMFQDEIEDCLRKHIQDAPEEFISELAEYLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTALTKHLLKTVCTDITNLMFNFLASDFLMAVEEPAAITSDIRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCEQLKVTEDPALILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVSQNKKTGQGEDPSSDELDKEQHDVTNATRKELQELSLSIKDLVLKSRKSSVTEE

Target ORF information:

RefSeq Version	NM_026194.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus UFM1 specific ligase 1 (Ufl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026194.4

ORF Insert Sequence:

ATGGCGGACG CCTGGGAGGA GATCCGGCGG CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCAGAGTCCA CGCAAAGGTT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTCCATACA CTTGACGGCA AGGAATATAT CACTCCAGCC 
CAAATCAGTA AAGAAATGAG AGACGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTT 
GACTTACAAC AGGTTATTAA TGTGGACCTG ACTCATATCG AGAGTAGAGT CAGTGATATT 
ATTAAATCAG AGAAGCACGT GCAGATGGTA CTGGGACAAC TGATTGATGA GAACTATCTG 
GATCAGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACTGTCTCA 
GAACTGTGTA AAGCCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTGGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTAG CTCGCCATAA AGCACGTATC CGCGGACTCT TCAGTGCCAT TACACGGCCT 
ACACCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTCGAGGACC TCGTCAGCAC TGGCCGCTTA CGAGGCACCG TGGTCGGCGG GCGACAGGAT 
AAAGCAGTCT TTGTCCCTGA CATCTACTCC AGGACACAGA GTACTTGGGT GGACTCCTTT 
TTCAGGCAGA ATGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCAACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GACTCGTAGA CCAGGTGGAA GCATCCGTAG AGGAAGCCAT CAGCTCAGGG 
ACCTGGGTTG ACATTTCGCC TCTGCTACCC AGTTCCTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTCCAGC AGGTGATGAG ACCATTCGGC AAGCTCGCTT CAGCTATAGT CTTTAGTGAC 
ACGGTTGTGG TGAGTGAAAA GTTCATAACT GACTGCACAG GACTGTTCAG CGAACGGATG 
CACCAAAAAG CTGAGAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC TGAAGAAGAT 
CTGAAACAAA TTTCCATTTT AGAAAGTGTT AATACAAGTA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGCTCTGGA AGTGTAAGAG GAGGAGGAGG GGGCAATGCC 
AGAGAATATA AAATTAAGAA AACCAAGAAG AAGGGAAGAA AAGATGAGGA CAGTGATGAT 
GAGTCCCAGT CGTCGCATGG TGGAAAGAAG AAGCCGGACA TCACATTCAT GTTCCAGGAT 
GAAATTGAAG ATTGCTTAAG GAAACACATT CAGGATGCCC CTGAGGAGTT TATTTCAGAG 
CTTGCAGAAT ACTTAATAAA GCCTCTTAAT AAAATGTATC TTGAGGTAGT ACGTTCAGTC 
TTCATGTCTT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACAATCAA GGACTTACAA 
GAAGAGGTTT CAAACCTATA CAATAATATC AGATTATTTG AAAAGGGAAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTTACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GATTTCCTCA TGGCAGTGGA GGAGCCTGCA 
GCCATTACAA GTGATATAAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACCAAAGTA 
GCTCTCACAA AACTCCATAA CTCTCTCAAT GAAAAAAGCA TAGAAGACTT CCTGTCTTGC 
CTGGATTCTG CCACAGAAGC GTGTGACATT ATGGTGAAAA AGGGAGACAA GAAAAGGGAA 
AGACAAATCC TCTTCCAGCA TCGACAGGCT CTGTGTGAGC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACACCT CACAGCAGTC CTGTTATTTC AGCTCTCAAC CCACAGCATG 
CTCCACGCAC CTGGAAGGTG TGTCCCACAG ATCATCGCGT TTCTTCACAG CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGTTTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGACTGGGCA GGGAGAGGAT CCGTCAAGCG ATGAATTAGA CAAGGAACAA 
CATGATGTCA CAAACGCTAC TCGGAAAGAG CTTCAGGAAC TGTCATTATC CATTAAGGAC 
CTTGTTCTCA AGTCCAGGAA ATCATCTGTC ACAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ufl1 ( NM_026194.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080470.2 Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant 1, mRNA.

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CloneID	OMu15746
Clone ID Related Accession (Same CDS sequence)	NM_001355512.1 , XM_006538203.3 , NM_026194.4 , NM_026194.5
Accession Version	NM_026194.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Mus musculus(house mouse)
Product	E3 UFM1-protein ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY097965.1, AK132697.1, AL935267.13 and BM900014.1. On Sep 19, 2017 this sequence version replaced NM_026194.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK132697.1, AK007993.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG CCTGGGAGGA GATCCGGCGG CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCAGAGTCCA CGCAAAGGTT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTCCATACA CTTGACGGCA AGGAATATAT CACTCCAGCC 
CAAATCAGTA AAGAAATGAG AGACGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTT 
GACTTACAAC AGGTTATTAA TGTGGACCTG ACTCATATCG AGAGTAGAGT CAGTGATATT 
ATTAAATCAG AGAAGCACGT GCAGATGGTA CTGGGACAAC TGATTGATGA GAACTATCTG 
GATCAGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACTGTCTCA 
GAACTGTGTA AAGCCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTGGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTAG CTCGCCATAA AGCACGTATC CGCGGACTCT TCAGTGCCAT TACACGGCCT 
ACACCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTCGAGGACC TCGTCAGCAC TGGCCGCTTA CGAGGCACCG TGGTCGGCGG GCGACAGGAT 
AAAGCAGTCT TTGTCCCTGA CATCTACTCC AGGACACAGA GTACTTGGGT GGACTCCTTT 
TTCAGGCAGA ATGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCAACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GACTCGTAGA CCAGGTGGAA GCATCCGTAG AGGAAGCCAT CAGCTCAGGG 
ACCTGGGTTG ACATTTCGCC TCTGCTACCC AGTTCCTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTCCAGC AGGTGATGAG ACCATTCGGC AAGCTCGCTT CAGCTATAGT CTTTAGTGAC 
ACGGTTGTGG TGAGTGAAAA GTTCATAACT GACTGCACAG GACTGTTCAG CGAACGGATG 
CACCAAAAAG CTGAGAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC TGAAGAAGAT 
CTGAAACAAA TTTCCATTTT AGAAAGTGTT AATACAAGTA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGCTCTGGA AGTGTAAGAG GAGGAGGAGG GGGCAATGCC 
AGAGAATATA AAATTAAGAA AACCAAGAAG AAGGGAAGAA AAGATGAGGA CAGTGATGAT 
GAGTCCCAGT CGTCGCATGG TGGAAAGAAG AAGCCGGACA TCACATTCAT GTTCCAGGAT 
GAAATTGAAG ATTGCTTAAG GAAACACATT CAGGATGCCC CTGAGGAGTT TATTTCAGAG 
CTTGCAGAAT ACTTAATAAA GCCTCTTAAT AAAATGTATC TTGAGGTAGT ACGTTCAGTC 
TTCATGTCTT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACAATCAA GGACTTACAA 
GAAGAGGTTT CAAACCTATA CAATAATATC AGATTATTTG AAAAGGGAAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTTACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GATTTCCTCA TGGCAGTGGA GGAGCCTGCA 
GCCATTACAA GTGATATAAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACCAAAGTA 
GCTCTCACAA AACTCCATAA CTCTCTCAAT GAAAAAAGCA TAGAAGACTT CCTGTCTTGC 
CTGGATTCTG CCACAGAAGC GTGTGACATT ATGGTGAAAA AGGGAGACAA GAAAAGGGAA 
AGACAAATCC TCTTCCAGCA TCGACAGGCT CTGTGTGAGC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACACCT CACAGCAGTC CTGTTATTTC AGCTCTCAAC CCACAGCATG 
CTCCACGCAC CTGGAAGGTG TGTCCCACAG ATCATCGCGT TTCTTCACAG CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGTTTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGACTGGGCA GGGAGAGGAT CCGTCAAGCG ATGAATTAGA CAAGGAACAA 
CATGATGTCA CAAACGCTAC TCGGAAAGAG CTTCAGGAAC TGTCATTATC CATTAAGGAC 
CTTGTTCTCA AGTCCAGGAA ATCATCTGTC ACAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080470.2
CDS	70..2451
Translation	MADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKYGFQEQLLYSVLEDLVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTQLLFLKATCVGQGLVDQVEASVEEAISSGTWVDISPLLPSSLSVEDAAMLLQQVMRPFGKLASAIVFSDTVVVSEKFITDCTGLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTSKKDKKDERRKKATEGSGSVRGGGGGNAREYKIKKTKKKGRKDEDSDDESQSSHGGKKKPDITFMFQDEIEDCLRKHIQDAPEEFISELAEYLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTALTKHLLKTVCTDITNLMFNFLASDFLMAVEEPAAITSDIRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCEQLKVTEDPALILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVSQNKKTGQGEDPSSDELDKEQHDVTNATRKELQELSLSIKDLVLKSRKSSVTEE

Target ORF information:

RefSeq Version	NM_026194.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026194.5

ORF Insert Sequence:

ATGGCGGACG CCTGGGAGGA GATCCGGCGG CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCAGAGTCCA CGCAAAGGTT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTCCATACA CTTGACGGCA AGGAATATAT CACTCCAGCC 
CAAATCAGTA AAGAAATGAG AGACGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTT 
GACTTACAAC AGGTTATTAA TGTGGACCTG ACTCATATCG AGAGTAGAGT CAGTGATATT 
ATTAAATCAG AGAAGCACGT GCAGATGGTA CTGGGACAAC TGATTGATGA GAACTATCTG 
GATCAGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACTGTCTCA 
GAACTGTGTA AAGCCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTGGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTAG CTCGCCATAA AGCACGTATC CGCGGACTCT TCAGTGCCAT TACACGGCCT 
ACACCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTCGAGGACC TCGTCAGCAC TGGCCGCTTA CGAGGCACCG TGGTCGGCGG GCGACAGGAT 
AAAGCAGTCT TTGTCCCTGA CATCTACTCC AGGACACAGA GTACTTGGGT GGACTCCTTT 
TTCAGGCAGA ATGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCAACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GACTCGTAGA CCAGGTGGAA GCATCCGTAG AGGAAGCCAT CAGCTCAGGG 
ACCTGGGTTG ACATTTCGCC TCTGCTACCC AGTTCCTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTCCAGC AGGTGATGAG ACCATTCGGC AAGCTCGCTT CAGCTATAGT CTTTAGTGAC 
ACGGTTGTGG TGAGTGAAAA GTTCATAACT GACTGCACAG GACTGTTCAG CGAACGGATG 
CACCAAAAAG CTGAGAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC TGAAGAAGAT 
CTGAAACAAA TTTCCATTTT AGAAAGTGTT AATACAAGTA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGCTCTGGA AGTGTAAGAG GAGGAGGAGG GGGCAATGCC 
AGAGAATATA AAATTAAGAA AACCAAGAAG AAGGGAAGAA AAGATGAGGA CAGTGATGAT 
GAGTCCCAGT CGTCGCATGG TGGAAAGAAG AAGCCGGACA TCACATTCAT GTTCCAGGAT 
GAAATTGAAG ATTGCTTAAG GAAACACATT CAGGATGCCC CTGAGGAGTT TATTTCAGAG 
CTTGCAGAAT ACTTAATAAA GCCTCTTAAT AAAATGTATC TTGAGGTAGT ACGTTCAGTC 
TTCATGTCTT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACAATCAA GGACTTACAA 
GAAGAGGTTT CAAACCTATA CAATAATATC AGATTATTTG AAAAGGGAAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTTACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GATTTCCTCA TGGCAGTGGA GGAGCCTGCA 
GCCATTACAA GTGATATAAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACCAAAGTA 
GCTCTCACAA AACTCCATAA CTCTCTCAAT GAAAAAAGCA TAGAAGACTT CCTGTCTTGC 
CTGGATTCTG CCACAGAAGC GTGTGACATT ATGGTGAAAA AGGGAGACAA GAAAAGGGAA 
AGACAAATCC TCTTCCAGCA TCGACAGGCT CTGTGTGAGC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACACCT CACAGCAGTC CTGTTATTTC AGCTCTCAAC CCACAGCATG 
CTCCACGCAC CTGGAAGGTG TGTCCCACAG ATCATCGCGT TTCTTCACAG CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGTTTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGACTGGGCA GGGAGAGGAT CCGTCAAGCG ATGAATTAGA CAAGGAACAA 
CATGATGTCA CAAACGCTAC TCGGAAAGAG CTTCAGGAAC TGTCATTATC CATTAAGGAC 
CTTGTTCTCA AGTCCAGGAA ATCATCTGTC ACAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ufl1 ( XM_006538203.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538266.1 Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15746
Clone ID Related Accession (Same CDS sequence)	NM_001355512.1 , XM_006538203.3 , NM_026194.4 , NM_026194.5
Accession Version	XM_006538203.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	E3 UFM1-protein ligase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538203.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG CCTGGGAGGA GATCCGGCGG CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCAGAGTCCA CGCAAAGGTT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTCCATACA CTTGACGGCA AGGAATATAT CACTCCAGCC 
CAAATCAGTA AAGAAATGAG AGACGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTT 
GACTTACAAC AGGTTATTAA TGTGGACCTG ACTCATATCG AGAGTAGAGT CAGTGATATT 
ATTAAATCAG AGAAGCACGT GCAGATGGTA CTGGGACAAC TGATTGATGA GAACTATCTG 
GATCAGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACTGTCTCA 
GAACTGTGTA AAGCCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTGGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTAG CTCGCCATAA AGCACGTATC CGCGGACTCT TCAGTGCCAT TACACGGCCT 
ACACCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTCGAGGACC TCGTCAGCAC TGGCCGCTTA CGAGGCACCG TGGTCGGCGG GCGACAGGAT 
AAAGCAGTCT TTGTCCCTGA CATCTACTCC AGGACACAGA GTACTTGGGT GGACTCCTTT 
TTCAGGCAGA ATGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCAACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GACTCGTAGA CCAGGTGGAA GCATCCGTAG AGGAAGCCAT CAGCTCAGGG 
ACCTGGGTTG ACATTTCGCC TCTGCTACCC AGTTCCTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTCCAGC AGGTGATGAG ACCATTCGGC AAGCTCGCTT CAGCTATAGT CTTTAGTGAC 
ACGGTTGTGG TGAGTGAAAA GTTCATAACT GACTGCACAG GACTGTTCAG CGAACGGATG 
CACCAAAAAG CTGAGAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC TGAAGAAGAT 
CTGAAACAAA TTTCCATTTT AGAAAGTGTT AATACAAGTA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGCTCTGGA AGTGTAAGAG GAGGAGGAGG GGGCAATGCC 
AGAGAATATA AAATTAAGAA AACCAAGAAG AAGGGAAGAA AAGATGAGGA CAGTGATGAT 
GAGTCCCAGT CGTCGCATGG TGGAAAGAAG AAGCCGGACA TCACATTCAT GTTCCAGGAT 
GAAATTGAAG ATTGCTTAAG GAAACACATT CAGGATGCCC CTGAGGAGTT TATTTCAGAG 
CTTGCAGAAT ACTTAATAAA GCCTCTTAAT AAAATGTATC TTGAGGTAGT ACGTTCAGTC 
TTCATGTCTT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACAATCAA GGACTTACAA 
GAAGAGGTTT CAAACCTATA CAATAATATC AGATTATTTG AAAAGGGAAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTTACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GATTTCCTCA TGGCAGTGGA GGAGCCTGCA 
GCCATTACAA GTGATATAAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACCAAAGTA 
GCTCTCACAA AACTCCATAA CTCTCTCAAT GAAAAAAGCA TAGAAGACTT CCTGTCTTGC 
CTGGATTCTG CCACAGAAGC GTGTGACATT ATGGTGAAAA AGGGAGACAA GAAAAGGGAA 
AGACAAATCC TCTTCCAGCA TCGACAGGCT CTGTGTGAGC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACACCT CACAGCAGTC CTGTTATTTC AGCTCTCAAC CCACAGCATG 
CTCCACGCAC CTGGAAGGTG TGTCCCACAG ATCATCGCGT TTCTTCACAG CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGTTTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGACTGGGCA GGGAGAGGAT CCGTCAAGCG ATGAATTAGA CAAGGAACAA 
CATGATGTCA CAAACGCTAC TCGGAAAGAG CTTCAGGAAC TGTCATTATC CATTAAGGAC 
CTTGTTCTCA AGTCCAGGAA ATCATCTGTC ACAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538266.1
CDS	179..2560
Translation	MADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQLSEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKYGFQEQLLYSVLEDLVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTQLLFLKATCVGQGLVDQVEASVEEAISSGTWVDISPLLPSSLSVEDAAMLLQQVMRPFGKLASAIVFSDTVVVSEKFITDCTGLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTSKKDKKDERRKKATEGSGSVRGGGGGNAREYKIKKTKKKGRKDEDSDDESQSSHGGKKKPDITFMFQDEIEDCLRKHIQDAPEEFISELAEYLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTALTKHLLKTVCTDITNLMFNFLASDFLMAVEEPAAITSDIRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCEQLKVTEDPALILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVSQNKKTGQGEDPSSDELDKEQHDVTNATRKELQELSLSIKDLVLKSRKSSVTEE

Target ORF information:

RefSeq Version	XM_006538203.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus UFM1 specific ligase 1 (Ufl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538203.3

ORF Insert Sequence:

ATGGCGGACG CCTGGGAGGA GATCCGGCGG CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCAGAGTCCA CGCAAAGGTT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTCCATACA CTTGACGGCA AGGAATATAT CACTCCAGCC 
CAAATCAGTA AAGAAATGAG AGACGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTT 
GACTTACAAC AGGTTATTAA TGTGGACCTG ACTCATATCG AGAGTAGAGT CAGTGATATT 
ATTAAATCAG AGAAGCACGT GCAGATGGTA CTGGGACAAC TGATTGATGA GAACTATCTG 
GATCAGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACTGTCTCA 
GAACTGTGTA AAGCCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTGGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTAG CTCGCCATAA AGCACGTATC CGCGGACTCT TCAGTGCCAT TACACGGCCT 
ACACCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTCGAGGACC TCGTCAGCAC TGGCCGCTTA CGAGGCACCG TGGTCGGCGG GCGACAGGAT 
AAAGCAGTCT TTGTCCCTGA CATCTACTCC AGGACACAGA GTACTTGGGT GGACTCCTTT 
TTCAGGCAGA ATGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCAACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GACTCGTAGA CCAGGTGGAA GCATCCGTAG AGGAAGCCAT CAGCTCAGGG 
ACCTGGGTTG ACATTTCGCC TCTGCTACCC AGTTCCTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTCCAGC AGGTGATGAG ACCATTCGGC AAGCTCGCTT CAGCTATAGT CTTTAGTGAC 
ACGGTTGTGG TGAGTGAAAA GTTCATAACT GACTGCACAG GACTGTTCAG CGAACGGATG 
CACCAAAAAG CTGAGAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC TGAAGAAGAT 
CTGAAACAAA TTTCCATTTT AGAAAGTGTT AATACAAGTA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGCTCTGGA AGTGTAAGAG GAGGAGGAGG GGGCAATGCC 
AGAGAATATA AAATTAAGAA AACCAAGAAG AAGGGAAGAA AAGATGAGGA CAGTGATGAT 
GAGTCCCAGT CGTCGCATGG TGGAAAGAAG AAGCCGGACA TCACATTCAT GTTCCAGGAT 
GAAATTGAAG ATTGCTTAAG GAAACACATT CAGGATGCCC CTGAGGAGTT TATTTCAGAG 
CTTGCAGAAT ACTTAATAAA GCCTCTTAAT AAAATGTATC TTGAGGTAGT ACGTTCAGTC 
TTCATGTCTT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACAATCAA GGACTTACAA 
GAAGAGGTTT CAAACCTATA CAATAATATC AGATTATTTG AAAAGGGAAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTTACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GATTTCCTCA TGGCAGTGGA GGAGCCTGCA 
GCCATTACAA GTGATATAAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACCAAAGTA 
GCTCTCACAA AACTCCATAA CTCTCTCAAT GAAAAAAGCA TAGAAGACTT CCTGTCTTGC 
CTGGATTCTG CCACAGAAGC GTGTGACATT ATGGTGAAAA AGGGAGACAA GAAAAGGGAA 
AGACAAATCC TCTTCCAGCA TCGACAGGCT CTGTGTGAGC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACACCT CACAGCAGTC CTGTTATTTC AGCTCTCAAC CCACAGCATG 
CTCCACGCAC CTGGAAGGTG TGTCCCACAG ATCATCGCGT TTCTTCACAG CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGTTTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGACTGGGCA GGGAGAGGAT CCGTCAAGCG ATGAATTAGA CAAGGAACAA 
CATGATGTCA CAAACGCTAC TCGGAAAGAG CTTCAGGAAC TGTCATTATC CATTAAGGAC 
CTTGTTCTCA AGTCCAGGAA ATCATCTGTC ACAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RCAD/BiP pathway is necessary for the proper synthesis of digestive enzymes and secretory function of the exocrine pancreas.
American journal of physiology. Gastrointestinal and liver physiology312(3)G314-G326(2017 Mar)
Miller C,Cai Y,Patton T,Graves SH,Li H,Sabbatini ME

RCAD/Ufl1, a Ufm1 E3 ligase, is essential for hematopoietic stem cell function and murine hematopoiesis.
Cell death and differentiation22(12)1922-34(2015 Dec)
Zhang M,Zhu X,Zhang Y,Cai Y,Chen J,Sivaprakasam S,Gurav A,Pi W,Makala L,Wu J,Pace B,Tuan-Lo D,Ganapathy V,Singh N,Li H

Transcriptional regulation of the Ufm1 conjugation system in response to disturbance of the endoplasmic reticulum homeostasis and inhibition of vesicle trafficking.
PloS one7(11)e48587(2012)
Zhang Y,Zhang M,Wu J,Lei G,Li H

Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis.
PloS one6(4)e18517(2011 Apr)
Lemaire K,Moura RF,Granvik M,Igoillo-Esteve M,Hohmeier HE,Hendrickx N,Newgard CB,Waelkens E,Cnop M,Schuit F

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Ufl1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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