Pdcl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdcl
Entrez Gene ID	67466
Full Name	phosducin-like
Synonyms	1200011E13Rik,AW108059,PhLP1
General protein information	Preferred Names phosducin-like Names phosducin-like protein PHLP
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 B

mRNA and Protein(s)

mRNA	Protein	Name
NM_026176.3	NP_080452.2	phosducin-like protein
XM_006498252.1	XP_006498315.1	phosducin-like protein isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDCL	NP_005379.3
Danio rerio (zebrafish)	pdcl	NP_955941.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004468	XP_313766.4
Xenopus tropicalis (tropical clawed frog)	pdcl	NP_001016070.1
Rattus norvegicus (Norway rat)	Pdcl	NP_071583.1
	PDCL	XP_001086958.1
Mus musculus (house mouse)	Pdcl	NP_080452.2
Gallus gallus (chicken)	PDCL	XP_004946061.1
Bos taurus (cattle)	PDCL	NP_777184.2
Pan troglodytes (chimpanzee)	PDCL	XP_528422.1
Canis lupus familiaris (dog)	PDCL	XP_005625456.1
Drosophila melanogaster (fruit fly)	CG7650	NP_648786.1

Gene Ontology
Bibliography

Related articles in PubMed

CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog.
Pusapati GV, Kong JH, Patel BB, Krishnan A, Sagner A, Kinnebrew M, Briscoe J, Aravind L, Rohatgi R
Developmental cell44(1)113-129.e8(2018 Jan)

Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling.
Xie K, Masuho I, Shih CC, Cao Y, Sasaki K, Lai CW, Han PL, Ueda H, Dessauer CW, Ehrlich ME, Xu B, Willardson BM, Martemyanov KA
eLife4(2015 Nov)

Retinal cone photoreceptors require phosducin-like protein 1 for G protein complex assembly and signaling.
Tracy CM, Kolesnikov AV, Blake DR, Chen CK, Baehr W, Kefalov VJ, Willardson BM
PloS one10(2)e0117129(2015)

Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins.
Gao X, Sinha S, Belcastro M, Woodard C, Ramamurthy V, Stoilov P, Sokolov M
The Journal of biological chemistry288(36)25760-8(2013 Sep)

Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors.
Lai CW, Kolesnikov AV, Frederick JM, Blake DR, Jiang L, Stewart JS, Chen CK, Barrow JR, Baehr W, Kefalov VJ, Willardson BM
The Journal of neuroscience : the official journal of the Society for Neuroscience33(18)7941-51(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings reveal a common mechanism of Gbetagamma and RGS9-Gbeta5 assembly in rods and cones, highlighting the importance of PhLP1 and CCT-mediated Gbeta complex formation in G protein signaling.
Title: Retinal cone photoreceptors require phosducin-like protein 1 for G protein complex assembly and signaling.

evidence of a generic mechanism, whereby the splicing of the PhLP gene could potentially and efficiently regulate the cellular levels of heterotrimeric G proteins.
Title: Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins.

this study demonstrated in vivo that PhLP1 is required for the folding and assembly of both Gbetagamma and Gbeta5-RGS9.
Title: Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors.

These results suggest a mechanism for Gbetagamma assembly in which PhLP stabilizes the nascent Gbeta polypeptide until Ggamma can associate, resulting in membrane binding of Gbetagamma and release of PhLP to catalyze another round of assembly.
Title: Phosducin-like protein acts as a molecular chaperone for G protein betagamma dimer assembly.

physiological control of G-protein regulation by PhLP seems to involve phosphorylation by CK2 and alternative splicing of the regulator
Title: Regulation of phosducin-like protein by casein kinase 2 and N-terminal splicing.

The following Pdcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01743	NM_026176.3 Latest version!	Mus musculus phosducin-like (Pdcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu01743	XM_006498252.1 Latest version!	Mus musculus phosducin-like (Pdcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdcl ( NM_026176.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080452.2 Mus musculus phosducin-like (Pdcl), mRNA.

Price & Availability↑

CloneID	OMu01743
Clone ID Related Accession (Same CDS sequence)	NM_026176.3 , XM_006498252.1
Accession Version	NM_026176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Mus musculus(house mouse)
Product	phosducin-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY024444.1, AK028668.1 and AV286628.1. On Jan 18, 2008 this sequence version replaced NM_026176.2. ##Evidence-Data-START## Transcript exon combination :: BC006578.1, AK133747.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGACAACCC TGGATGATAA GTTACTGGGG GAGAAATTAC AGTACTACTA CAGCACCAGC 
GAGGATGAGG ACAGTGACCA TGAAGACAAA GACAGAGGCA GGGGAGCACC AGCCATTAGT 
TCCACACCTG CAGAGGCTGA GCTGGCAGGC GAAGGCATCT CCATCAATAC AGGTCCAAAA 
GGTGTGATCA ATGACTGGCG CCGCTTCAAG CAGTTGGAGA CAGAGCAGAG GGAGGAGCAG 
TGCCGGGAGA TGGAGCGGCT GATCAAAAAG CTGTCTATGA GCTGCAGGTC CCATCTGGAT 
GAAGAGGAGG AGCAACAGAA ACAGAAGGAC CTCCAGGAGA AAATCAGTGG GAAGATGACT 
CTAAAGGAGT TTGGTACAAA GGACAAGAAC TTGGATGATG AAGAGTTTTT GCAGCAGTAT 
CGGAAGCAGA GGATGGAAGA GATGCGGCAG CAGTTTCATA AAGGGCCCCA ATTCAAGCAA 
GTTTTTGAAA TCCCCAGTGG AGAAGGGTTT TTAGATATGA TTGATAAAGA ACAAAAAAGC 
ACACTTATCA TGGTTCATAT TTATGAAGAT GGCGTCCCAG GGACTGAAGC CATGAATGGC 
TGCATGATCT GCCTTGCCAC AGAGTACCCC GCCGTCAAAT TCTGTCGGGT GAGGAGCTCA 
GTTATTGGGG CCAGTAGTCG TTTTACCCGG AATGCCCTTC CTGCTCTGCT CATCTACAAG 
GCGGGTGAAT TGATTGGCAA TTTTGTTCGA GTCACTGACC AGCTGGGGGA AGATTTCTTT 
GCTGTAGACC TTGAAGCTTT CCTGCAGGAA TTTGGATTGC TCCCAGAAAA GGAAGTCTTG 
GTGCTGACAT CTGTGCGAAA CTCTGCCACC TGTCACAGTG AAGACAGCGA TCTGGAAATA 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080452.2
CDS	196..1101
Translation	MTTLDDKLLGEKLQYYYSTSEDEDSDHEDKDRGRGAPAISSTPAEAELAGEGISINTGPKGVINDWRRFKQLETEQREEQCREMERLIKKLSMSCRSHLDEEEEQQKQKDLQEKISGKMTLKEFGTKDKNLDDEEFLQQYRKQRMEEMRQQFHKGPQFKQVFEIPSGEGFLDMIDKEQKSTLIMVHIYEDGVPGTEAMNGCMICLATEYPAVKFCRVRSSVIGASSRFTRNALPALLIYKAGELIGNFVRVTDQLGEDFFAVDLEAFLQEFGLLPEKEVLVLTSVRNSATCHSEDSDLEID

Target ORF information:

RefSeq Version	NM_026176.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosducin-like (Pdcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026176.3

ORF Insert Sequence:

ATGACAACCC TGGATGATAA GTTACTGGGG GAGAAATTAC AGTACTACTA CAGCACCAGC 
GAGGATGAGG ACAGTGACCA TGAAGACAAA GACAGAGGCA GGGGAGCACC AGCCATTAGT 
TCCACACCTG CAGAGGCTGA GCTGGCAGGC GAAGGCATCT CCATCAATAC AGGTCCAAAA 
GGTGTGATCA ATGACTGGCG CCGCTTCAAG CAGTTGGAGA CAGAGCAGAG GGAGGAGCAG 
TGCCGGGAGA TGGAGCGGCT GATCAAAAAG CTGTCTATGA GCTGCAGGTC CCATCTGGAT 
GAAGAGGAGG AGCAACAGAA ACAGAAGGAC CTCCAGGAGA AAATCAGTGG GAAGATGACT 
CTAAAGGAGT TTGGTACAAA GGACAAGAAC TTGGATGATG AAGAGTTTTT GCAGCAGTAT 
CGGAAGCAGA GGATGGAAGA GATGCGGCAG CAGTTTCATA AAGGGCCCCA ATTCAAGCAA 
GTTTTTGAAA TCCCCAGTGG AGAAGGGTTT TTAGATATGA TTGATAAAGA ACAAAAAAGC 
ACACTTATCA TGGTTCATAT TTATGAAGAT GGCGTCCCAG GGACTGAAGC CATGAATGGC 
TGCATGATCT GCCTTGCCAC AGAGTACCCC GCCGTCAAAT TCTGTCGGGT GAGGAGCTCA 
GTTATTGGGG CCAGTAGTCG TTTTACCCGG AATGCCCTTC CTGCTCTGCT CATCTACAAG 
GCGGGTGAAT TGATTGGCAA TTTTGTTCGA GTCACTGACC AGCTGGGGGA AGATTTCTTT 
GCTGTAGACC TTGAAGCTTT CCTGCAGGAA TTTGGATTGC TCCCAGAAAA GGAAGTCTTG 
GTGCTGACAT CTGTGCGAAA CTCTGCCACC TGTCACAGTG AAGACAGCGA TCTGGAAATA 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdcl ( XM_006498252.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498315.1 Mus musculus phosducin-like (Pdcl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu01743
Clone ID Related Accession (Same CDS sequence)	NM_026176.3 , XM_006498252.1
Accession Version	XM_006498252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosducin-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCC TGGATGATAA GTTACTGGGG GAGAAATTAC AGTACTACTA CAGCACCAGC 
GAGGATGAGG ACAGTGACCA TGAAGACAAA GACAGAGGCA GGGGAGCACC AGCCATTAGT 
TCCACACCTG CAGAGGCTGA GCTGGCAGGC GAAGGCATCT CCATCAATAC AGGTCCAAAA 
GGTGTGATCA ATGACTGGCG CCGCTTCAAG CAGTTGGAGA CAGAGCAGAG GGAGGAGCAG 
TGCCGGGAGA TGGAGCGGCT GATCAAAAAG CTGTCTATGA GCTGCAGGTC CCATCTGGAT 
GAAGAGGAGG AGCAACAGAA ACAGAAGGAC CTCCAGGAGA AAATCAGTGG GAAGATGACT 
CTAAAGGAGT TTGGTACAAA GGACAAGAAC TTGGATGATG AAGAGTTTTT GCAGCAGTAT 
CGGAAGCAGA GGATGGAAGA GATGCGGCAG CAGTTTCATA AAGGGCCCCA ATTCAAGCAA 
GTTTTTGAAA TCCCCAGTGG AGAAGGGTTT TTAGATATGA TTGATAAAGA ACAAAAAAGC 
ACACTTATCA TGGTTCATAT TTATGAAGAT GGCGTCCCAG GGACTGAAGC CATGAATGGC 
TGCATGATCT GCCTTGCCAC AGAGTACCCC GCCGTCAAAT TCTGTCGGGT GAGGAGCTCA 
GTTATTGGGG CCAGTAGTCG TTTTACCCGG AATGCCCTTC CTGCTCTGCT CATCTACAAG 
GCGGGTGAAT TGATTGGCAA TTTTGTTCGA GTCACTGACC AGCTGGGGGA AGATTTCTTT 
GCTGTAGACC TTGAAGCTTT CCTGCAGGAA TTTGGATTGC TCCCAGAAAA GGAAGTCTTG 
GTGCTGACAT CTGTGCGAAA CTCTGCCACC TGTCACAGTG AAGACAGCGA TCTGGAAATA 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498315.1
CDS	731..1636
Translation	MTTLDDKLLGEKLQYYYSTSEDEDSDHEDKDRGRGAPAISSTPAEAELAGEGISINTGPKGVINDWRRFKQLETEQREEQCREMERLIKKLSMSCRSHLDEEEEQQKQKDLQEKISGKMTLKEFGTKDKNLDDEEFLQQYRKQRMEEMRQQFHKGPQFKQVFEIPSGEGFLDMIDKEQKSTLIMVHIYEDGVPGTEAMNGCMICLATEYPAVKFCRVRSSVIGASSRFTRNALPALLIYKAGELIGNFVRVTDQLGEDFFAVDLEAFLQEFGLLPEKEVLVLTSVRNSATCHSEDSDLEID

Target ORF information:

RefSeq Version	XM_006498252.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosducin-like (Pdcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498252.1

ORF Insert Sequence:

ATGACAACCC TGGATGATAA GTTACTGGGG GAGAAATTAC AGTACTACTA CAGCACCAGC 
GAGGATGAGG ACAGTGACCA TGAAGACAAA GACAGAGGCA GGGGAGCACC AGCCATTAGT 
TCCACACCTG CAGAGGCTGA GCTGGCAGGC GAAGGCATCT CCATCAATAC AGGTCCAAAA 
GGTGTGATCA ATGACTGGCG CCGCTTCAAG CAGTTGGAGA CAGAGCAGAG GGAGGAGCAG 
TGCCGGGAGA TGGAGCGGCT GATCAAAAAG CTGTCTATGA GCTGCAGGTC CCATCTGGAT 
GAAGAGGAGG AGCAACAGAA ACAGAAGGAC CTCCAGGAGA AAATCAGTGG GAAGATGACT 
CTAAAGGAGT TTGGTACAAA GGACAAGAAC TTGGATGATG AAGAGTTTTT GCAGCAGTAT 
CGGAAGCAGA GGATGGAAGA GATGCGGCAG CAGTTTCATA AAGGGCCCCA ATTCAAGCAA 
GTTTTTGAAA TCCCCAGTGG AGAAGGGTTT TTAGATATGA TTGATAAAGA ACAAAAAAGC 
ACACTTATCA TGGTTCATAT TTATGAAGAT GGCGTCCCAG GGACTGAAGC CATGAATGGC 
TGCATGATCT GCCTTGCCAC AGAGTACCCC GCCGTCAAAT TCTGTCGGGT GAGGAGCTCA 
GTTATTGGGG CCAGTAGTCG TTTTACCCGG AATGCCCTTC CTGCTCTGCT CATCTACAAG 
GCGGGTGAAT TGATTGGCAA TTTTGTTCGA GTCACTGACC AGCTGGGGGA AGATTTCTTT 
GCTGTAGACC TTGAAGCTTT CCTGCAGGAA TTTGGATTGC TCCCAGAAAA GGAAGTCTTG 
GTGCTGACAT CTGTGCGAAA CTCTGCCACC TGTCACAGTG AAGACAGCGA TCTGGAAATA 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog.
Developmental cell44(1)113-129.e8(2018 Jan)
Pusapati GV,Kong JH,Patel BB,Krishnan A,Sagner A,Kinnebrew M,Briscoe J,Aravind L,Rohatgi R

Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling.
eLife4(2015 Nov)
Xie K,Masuho I,Shih CC,Cao Y,Sasaki K,Lai CW,Han PL,Ueda H,Dessauer CW,Ehrlich ME,Xu B,Willardson BM,Martemyanov KA

Retinal cone photoreceptors require phosducin-like protein 1 for G protein complex assembly and signaling.
PloS one10(2)e0117129(2015)
Tracy CM,Kolesnikov AV,Blake DR,Chen CK,Baehr W,Kefalov VJ,Willardson BM

Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins.
The Journal of biological chemistry288(36)25760-8(2013 Sep)
Gao X,Sinha S,Belcastro M,Woodard C,Ramamurthy V,Stoilov P,Sokolov M

Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(18)7941-51(2013 May)
Lai CW,Kolesnikov AV,Frederick JM,Blake DR,Jiang L,Stewart JS,Chen CK,Barrow JR,Baehr W,Kefalov VJ,Willardson BM

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Pdcl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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