RPS20 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPS20
Entrez Gene ID	67427
Full Name	ribosomal protein S20
Synonyms	4632426K06Rik,Dsk4
General protein information	Preferred Names ribosomal protein S20 Names 40S ribosomal protein S20
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A1
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Dec 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_026147.6	NP_080423.1	40S ribosomal protein S20
NM_026147.5	NP_080423.1	40S ribosomal protein S20

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RPS20	XP_535079.2
Saccharomyces cerevisiae (baker's yeast)	RPS20	NP_011848.1
Oryza sativa (rice)	Os03g0249400	NP_001049560.1
Pan troglodytes (chimpanzee)	RPS20	XP_001154969.1
Rattus norvegicus (Norway rat)	Rps20	NP_001007604.1
Caenorhabditis elegans (roundworm)	rps-20	NP_740944.1
Kluyveromyces lactis	KLLA0F25542g	XP_456218.1
Schizosaccharomyces pombe (fission yeast)	rps20	NP_588436.1
Oryza sativa (rice)	Os06g0134000	NP_001056713.1
Xenopus tropicalis (tropical clawed frog)	rbm6	NP_001005106.1
	RPS20	XP_001085267.1
Bos taurus (cattle)	RPS20	NP_001029610.1
Mus musculus (house mouse)	Rps20	NP_080423.1
Magnaporthe oryzae (rice blast fungus)	MGG_03372	XP_003716574.1
Homo sapiens (human)	RPS20	NP_001014.1
Danio rerio (zebrafish)	rps20	NP_998369.1
Eremothecium gossypii	AGOS_ABR041C	NP_982987.1
Neurospora crassa	NCU06892	XP_959239.1
Arabidopsis thaliana (thale cress)	AT5G62300	NP_201036.1
Arabidopsis thaliana (thale cress)	AT3G47370	NP_190321.1
Gallus gallus (chicken)	RPS20	XP_428540.3
Drosophila melanogaster (fruit fly)	RpS20	NP_524421.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010591	XP_314556.3
Arabidopsis thaliana (thale cress)	AT3G45030	NP_190089.2
Oryza sativa (rice)	Os10g0170200	NP_001064232.1

Gene Ontology
Bibliography

Related articles in PubMed

Long-range enhancers regulating Myc expression are required for normal facial morphogenesis.
Uslu VV, Petretich M, Ruf S, Langenfeld K, Fonseca NA, Marioni JC, Spitz F
Nature genetics46(7)753-8(2014 Jul)

Ribosome-mediated specificity in Hox mRNA translation and vertebrate tissue patterning.
Kondrashov N, Pusic A, Stumpf CR, Shimizu K, Hsieh AC, Ishijima J, Shiroishi T, Barna M
Cell145(3)383-397(2011 Apr)

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
Sansom SN, Griffiths DS, Faedo A, Kleinjan DJ, Ruan Y, Smith J, van Heyningen V, Rubenstein JL, Livesey FJ
PLoS genetics5(6)e1000511(2009 Jun)

Ribosomal mutations cause p53-mediated dark skin and pleiotropic effects.
McGowan KA, Li JZ, Park CY, Beaudry V, Tabor HK, Sabnis AJ, Zhang W, Fuchs H, de Angelis MH, Myers RM, Attardi LD, Barsh GS
Nature genetics40(8)963-70(2008 Aug)

Comprehensive identification of phosphorylation sites in postsynaptic density preparations.
Trinidad JC, Specht CG, Thalhammer A, Schoepfer R, Burlingame AL
Molecular & cellular proteomics : MCP5(5)914-22(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports two mouse dark skin (Dsk) loci caused by mutations in Rps19 (ribosomal protein S19) and Rps20 (ribosomal protein S20).
Title: Ribosomal mutations cause p53-mediated dark skin and pleiotropic effects.

The following RPS20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19512	NM_026147.6 Latest version!	Mus musculus ribosomal protein S20 (Rps20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu19512	NM_026147.5	Mus musculus ribosomal protein S20 (Rps20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPS20 ( NM_026147.6 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_080423.1 Mus musculus ribosomal protein S20 (Rps20), mRNA.

Price & Availability↑

CloneID	OMu19512
Clone ID Related Accession (Same CDS sequence)	NM_026147.6 , NM_026147.5
Accession Version	NM_026147.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	40S ribosomal protein S20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ041981.1, AK136398.1, AL807387.10 and AL772401.22. On Dec 4, 2015 this sequence version replaced NM_026147.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.69771.1, BY714044.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATTTA AAGATACCGG AAAGACGCCC GTGGAGCCCG AAGTGGCGAT TCACCGAATT 
CGAATCACGC TCACCAGCCG CAACGTGAAG TCGCTGGAGA AGGTTTGTGC GGACTTGATC 
AGAGGCGCCA AGGAAAAGAA TCTGAAAGTG AAAGGACCGG TGCGCATGCC TACCAAGACT 
TTGAGAATCA CTACCAGAAA AACACCTTGT GGTGAAGGTT CCAAGACCTG GGATCGATTC 
CAGATGAGGA TCCACAAGCG ACTCATTGAT TTACATAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCGGGAGTT GAGGTTGAAG TCACCATTGC AGATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080423.1
CDS	170..529
Translation	MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA

Target ORF information:

RefSeq Version	NM_026147.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribosomal protein S20 (Rps20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026147.6

ORF Insert Sequence:

ATGGCATTTA AAGATACCGG AAAGACGCCC GTGGAGCCCG AAGTGGCGAT TCACCGAATT 
CGAATCACGC TCACCAGCCG CAACGTGAAG TCGCTGGAGA AGGTTTGTGC GGACTTGATC 
AGAGGCGCCA AGGAAAAGAA TCTGAAAGTG AAAGGACCGG TGCGCATGCC TACCAAGACT 
TTGAGAATCA CTACCAGAAA AACACCTTGT GGTGAAGGTT CCAAGACCTG GGATCGATTC 
CAGATGAGGA TCCACAAGCG ACTCATTGAT TTACATAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCGGGAGTT GAGGTTGAAG TCACCATTGC AGATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPS20 ( NM_026147.5 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_080423.1 Mus musculus ribosomal protein S20 (Rps20), mRNA.

Price & Availability↑

CloneID	OMu19512
Clone ID Related Accession (Same CDS sequence)	NM_026147.6 , NM_026147.5
Accession Version	NM_026147.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	40S ribosomal protein S20
Comment

ATGGCATTTA AAGATACCGG AAAGACGCCC GTGGAGCCCG AAGTGGCGAT TCACCGAATT 
CGAATCACGC TCACCAGCCG CAACGTGAAG TCGCTGGAGA AGGTTTGTGC GGACTTGATC 
AGAGGCGCCA AGGAAAAGAA TCTGAAAGTG AAAGGACCGG TGCGCATGCC TACCAAGACT 
TTGAGAATCA CTACCAGAAA AACACCTTGT GGTGAAGGTT CCAAGACCTG GGATCGATTC 
CAGATGAGGA TCCACAAGCG ACTCATTGAT TTACATAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCGGGAGTT GAGGTTGAAG TCACCATTGC AGATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080423.1
Translation	MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA

Target ORF information:

RefSeq Version	NM_026147.5
Organism	Mus musculus(House mouse)
Definition	Mus musculus ribosomal protein S20 (Rps20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026147.5

ORF Insert Sequence:

ATGGCATTTA AAGATACCGG AAAGACGCCC GTGGAGCCCG AAGTGGCGAT TCACCGAATT 
CGAATCACGC TCACCAGCCG CAACGTGAAG TCGCTGGAGA AGGTTTGTGC GGACTTGATC 
AGAGGCGCCA AGGAAAAGAA TCTGAAAGTG AAAGGACCGG TGCGCATGCC TACCAAGACT 
TTGAGAATCA CTACCAGAAA AACACCTTGT GGTGAAGGTT CCAAGACCTG GGATCGATTC 
CAGATGAGGA TCCACAAGCG ACTCATTGAT TTACATAGTC CTTCTGAGAT TGTTAAGCAG 
ATTACTTCCA TCAGTATTGA GCCGGGAGTT GAGGTTGAAG TCACCATTGC AGATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Long-range enhancers regulating Myc expression are required for normal facial morphogenesis.
Nature genetics46(7)753-8(2014 Jul)
Uslu VV,Petretich M,Ruf S,Langenfeld K,Fonseca NA,Marioni JC,Spitz F

Ribosome-mediated specificity in Hox mRNA translation and vertebrate tissue patterning.
Cell145(3)383-397(2011 Apr)
Kondrashov N,Pusic A,Stumpf CR,Shimizu K,Hsieh AC,Ishijima J,Shiroishi T,Barna M

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
PLoS genetics5(6)e1000511(2009 Jun)
Sansom SN,Griffiths DS,Faedo A,Kleinjan DJ,Ruan Y,Smith J,van Heyningen V,Rubenstein JL,Livesey FJ

Ribosomal mutations cause p53-mediated dark skin and pleiotropic effects.
Nature genetics40(8)963-70(2008 Aug)
McGowan KA,Li JZ,Park CY,Beaudry V,Tabor HK,Sabnis AJ,Zhang W,Fuchs H,de Angelis MH,Myers RM,Attardi LD,Barsh GS

Comprehensive identification of phosphorylation sites in postsynaptic density preparations.
Molecular & cellular proteomics : MCP5(5)914-22(2006 May)
Trinidad JC,Specht CG,Thalhammer A,Schoepfer R,Burlingame AL

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RPS20 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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