Ift22 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ift22
Entrez Gene ID	67286
Full Name	intraflagellar transport 22
Synonyms	3110017O03Rik,AI835745,AI847427,Rabl5
General protein information	Preferred Names intraflagellar transport 22 Names intraflagellar transport protein 22 homolog RAB, member of RAS oncogene family-like 5 rab-like protein 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
XM_011240905.2	XP_011239207.1	intraflagellar transport protein 22 homolog isoform X1
XM_011240905.3	XP_011239207.1	intraflagellar transport protein 22 homolog isoform X1
NM_026073.3	NP_080349.1	intraflagellar transport protein 22 homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	IFT22	NP_073614.1
	RABL5	XP_001108384.1
Gallus gallus (chicken)	RABL5	XP_415752.2
Bos taurus (cattle)	RABL5	NP_001015612.1
Rattus norvegicus (Norway rat)	Rabl5	NP_001011902.1
Danio rerio (zebrafish)	rabl5	NP_001017884.1
Xenopus tropicalis (tropical clawed frog)	rabl5	NP_001011271.1
Pan troglodytes (chimpanzee)	RABL5	XP_003318735.1
Canis lupus familiaris (dog)	RABL5	XP_536853.3
Mus musculus (house mouse)	Rabl5	NP_080349.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP, Nachury MV
Developmental cell31(3)265-78(2014 Nov)

TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.
Ishikawa H, Ide T, Yagi T, Jiang X, Hirono M, Sasaki H, Yanagisawa H, Wemmer KA, Stainier DY, Qin H, Kamiya R, Marshall WF
eLife3e01566(2014 Jan)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Characterization of mouse IFT complex B.
Follit JA, Xu F, Keady BT, Pazour GJ
Cell motility and the cytoskeleton66(8)457-68(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ift22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23595	NM_026073.3 Latest version!	Mus musculus intraflagellar transport 22 (Ift22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu70110	XM_011240905.3 Latest version!	Mus musculus intraflagellar transport 22 (Ift22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu70110	XM_011240905.2	Mus musculus intraflagellar transport 22 (Ift22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ift22 ( NM_026073.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080349.1 Mus musculus intraflagellar transport 22 (Ift22), mRNA.

Price & Availability↑

CloneID	OMu23595
Clone ID Related Accession (Same CDS sequence)	NM_026073.3
Accession Version	NM_026073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 22 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC147987.5. On Nov 17, 2006 this sequence version replaced NM_026073.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC009150.1, SRR1660811.181028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAAGG CTAAGATCCT CTTCGTGGGC CCCTGCGAGA GCGGCAAAAC AGTGTTGGCC 
AACTTTCTCA CTGAATCTTC GGACATCACT GAATATAACC CAACACAAGG TGTGAGGATC 
CTAGAGTTTG AGAACCCACA TGTCACCAGC AACAACAAAG GCACGGGCTG CGAATTCGAG 
CTCTGGGACT GTGGTGGCGA CTCTAAGTTT GAGTCCTGTT GGCCCGCTCT GATGAAGGAT 
GCTCATGGAG TGGTGATCGT CTTCAATGCT GATATTCCAA GCCACTTAAA GGAAATTGAA 
ATGTGGTATT CCTGCTTTGT CCAGCAACAG TTCCTCCAAG ACAGTCACTG CATGCTTGTG 
GCCCACCACA AACCGGGCTC CGGAGGAGAA AGGGGCAGCC TGGCTTTGTC TCCCCCCTTG 
AACAAGCTGA AGCTGGTGCA CTCAAACCTG GAGGAAGACC CTGAGGAGGT CCGGGTGGAG 
TTCATCAAGT ATTTAAAAAG CATCATCAAC TCCATGTCAG AGAGCAGAGA CCGCGAGGAG 
ATGCTCATCA TCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080349.1
CDS	83..640
Translation	MLKAKILFVGPCESGKTVLANFLTESSDITEYNPTQGVRILEFENPHVTSNNKGTGCEFELWDCGGDSKFESCWPALMKDAHGVVIVFNADIPSHLKEIEMWYSCFVQQQFLQDSHCMLVAHHKPGSGGERGSLALSPPLNKLKLVHSNLEEDPEEVRVEFIKYLKSIINSMSESRDREEMLIIT

Target ORF information:

RefSeq Version	NM_026073.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 22 (Ift22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026073.3

ORF Insert Sequence:

ATGCTGAAGG CTAAGATCCT CTTCGTGGGC CCCTGCGAGA GCGGCAAAAC AGTGTTGGCC 
AACTTTCTCA CTGAATCTTC GGACATCACT GAATATAACC CAACACAAGG TGTGAGGATC 
CTAGAGTTTG AGAACCCACA TGTCACCAGC AACAACAAAG GCACGGGCTG CGAATTCGAG 
CTCTGGGACT GTGGTGGCGA CTCTAAGTTT GAGTCCTGTT GGCCCGCTCT GATGAAGGAT 
GCTCATGGAG TGGTGATCGT CTTCAATGCT GATATTCCAA GCCACTTAAA GGAAATTGAA 
ATGTGGTATT CCTGCTTTGT CCAGCAACAG TTCCTCCAAG ACAGTCACTG CATGCTTGTG 
GCCCACCACA AACCGGGCTC CGGAGGAGAA AGGGGCAGCC TGGCTTTGTC TCCCCCCTTG 
AACAAGCTGA AGCTGGTGCA CTCAAACCTG GAGGAAGACC CTGAGGAGGT CCGGGTGGAG 
TTCATCAAGT ATTTAAAAAG CATCATCAAC TCCATGTCAG AGAGCAGAGA CCGCGAGGAG 
ATGCTCATCA TCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift22 ( XM_011240905.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239207.1 Mus musculus intraflagellar transport 22 (Ift22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70110
Clone ID Related Accession (Same CDS sequence)	XM_011240905.2 , XM_011240905.3
Accession Version	XM_011240905.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 22 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGATG CTCATGGAGT GGTGATCGTC TTCAATGCTG ATATTCCAAG CCACTTAAAG 
GAAATTGAAA TGTGGTATTC CTGCTTTGTC CAGCAACAGT TCCTCCAAGA CAGTCACTGC 
ATGCTTGTGG CCCACCACAA ACCGGGCTCC GGAGGAGAAA GGGGCAGCCT GGCTTTGTCT 
CCCCCCTTGA ACAAGCTGAA GCTGGTGCAC TCAAACCTGG AGGAAGACCC TGAGGAGGTC 
CGGGTGGAGT TCATCAAGTA TTTAAAAAGC ATCATCAACT CCATGTCAGA GAGCAGAGAC 
CGCGAGGAGA TGCTCATCAT CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239207.1
CDS	880..1206
Translation	MKDAHGVVIVFNADIPSHLKEIEMWYSCFVQQQFLQDSHCMLVAHHKPGSGGERGSLALSPPLNKLKLVHSNLEEDPEEVRVEFIKYLKSIINSMSESRDREEMLIIT

Target ORF information:

RefSeq Version	XM_011240905.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 22 (Ift22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240905.2

ORF Insert Sequence:

ATGAAGGATG CTCATGGAGT GGTGATCGTC TTCAATGCTG ATATTCCAAG CCACTTAAAG 
GAAATTGAAA TGTGGTATTC CTGCTTTGTC CAGCAACAGT TCCTCCAAGA CAGTCACTGC 
ATGCTTGTGG CCCACCACAA ACCGGGCTCC GGAGGAGAAA GGGGCAGCCT GGCTTTGTCT 
CCCCCCTTGA ACAAGCTGAA GCTGGTGCAC TCAAACCTGG AGGAAGACCC TGAGGAGGTC 
CGGGTGGAGT TCATCAAGTA TTTAAAAAGC ATCATCAACT CCATGTCAGA GAGCAGAGAC 
CGCGAGGAGA TGCTCATCAT CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift22 ( XM_011240905.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239207.1 Mus musculus intraflagellar transport 22 (Ift22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70110
Clone ID Related Accession (Same CDS sequence)	XM_011240905.2 , XM_011240905.3
Accession Version	XM_011240905.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 22 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGATG CTCATGGAGT GGTGATCGTC TTCAATGCTG ATATTCCAAG CCACTTAAAG 
GAAATTGAAA TGTGGTATTC CTGCTTTGTC CAGCAACAGT TCCTCCAAGA CAGTCACTGC 
ATGCTTGTGG CCCACCACAA ACCGGGCTCC GGAGGAGAAA GGGGCAGCCT GGCTTTGTCT 
CCCCCCTTGA ACAAGCTGAA GCTGGTGCAC TCAAACCTGG AGGAAGACCC TGAGGAGGTC 
CGGGTGGAGT TCATCAAGTA TTTAAAAAGC ATCATCAACT CCATGTCAGA GAGCAGAGAC 
CGCGAGGAGA TGCTCATCAT CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239207.1
CDS	876..1202
Translation	MKDAHGVVIVFNADIPSHLKEIEMWYSCFVQQQFLQDSHCMLVAHHKPGSGGERGSLALSPPLNKLKLVHSNLEEDPEEVRVEFIKYLKSIINSMSESRDREEMLIIT

Target ORF information:

RefSeq Version	XM_011240905.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 22 (Ift22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240905.3

ORF Insert Sequence:

ATGAAGGATG CTCATGGAGT GGTGATCGTC TTCAATGCTG ATATTCCAAG CCACTTAAAG 
GAAATTGAAA TGTGGTATTC CTGCTTTGTC CAGCAACAGT TCCTCCAAGA CAGTCACTGC 
ATGCTTGTGG CCCACCACAA ACCGGGCTCC GGAGGAGAAA GGGGCAGCCT GGCTTTGTCT 
CCCCCCTTGA ACAAGCTGAA GCTGGTGCAC TCAAACCTGG AGGAAGACCC TGAGGAGGTC 
CGGGTGGAGT TCATCAAGTA TTTAAAAAGC ATCATCAACT CCATGTCAGA GAGCAGAGAC 
CGCGAGGAGA TGCTCATCAT CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
Developmental cell31(3)265-78(2014 Nov)
Liew GM,Ye F,Nager AR,Murphy JP,Lee JS,Aguiar M,Breslow DK,Gygi SP,Nachury MV

TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.
eLife3e01566(2014 Jan)
Ishikawa H,Ide T,Yagi T,Jiang X,Hirono M,Sasaki H,Yanagisawa H,Wemmer KA,Stainier DY,Qin H,Kamiya R,Marshall WF

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Characterization of mouse IFT complex B.
Cell motility and the cytoskeleton66(8)457-68(2009 Aug)
Follit JA,Xu F,Keady BT,Pazour GJ

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Ift22 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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