Naaa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Naaa
Entrez Gene ID	67111
Full Name	N-acylethanolamine acid amidase
Synonyms	2210023K21Rik,3830414F09Rik,Asahl
General protein information	Preferred Names N-acylethanolamine acid amidase Names N-acylethanolamine-hydrolyzing acid amidase ASAH-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163687.1	NP_001157159.1	N-acylethanolamine-hydrolyzing acid amidase isoform 2 precursor
NM_025972.4	NP_080248.2	N-acylethanolamine-hydrolyzing acid amidase isoform 1 precursor

Pathways from BioSystems

Homologies

	NAAA	XP_001099747.1
Bos taurus (cattle)	NAAA	NP_001093839.1
Mus musculus (house mouse)	Naaa	NP_080248.2
Rattus norvegicus (Norway rat)	Naaa	NP_001010967.1
Canis lupus familiaris (dog)	NAAA	XP_003640161.1
Caenorhabditis elegans (roundworm)	Y55D5A.3	NP_497647.1
Homo sapiens (human)	NAAA	NP_055250.2
Pan troglodytes (chimpanzee)	NAAA	XP_001152628.2
Gallus gallus (chicken)	NAAA	XP_420596.2
Xenopus tropicalis (tropical clawed frog)	naaa	NP_001005714.1

Gene Ontology
Bibliography

Related articles in PubMed

The N-acylethanolamine acid amidase inhibitor ARN077 suppresses inflammation and pruritus in a mouse model of allergic dermatitis.
Sasso O, Summa M, Armirotti A, Pontis S, De Mei C, Piomelli D
The Journal of investigative dermatology(2017 Oct)

N-acylethanolamine-hydrolyzing acid amidase and fatty acid amide hydrolase inhibition differentially affect N-acylethanolamine levels and macrophage activation.
Alhouayek M, Bottemanne P, Makriyannis A, Muccioli GG
Biochimica et biophysica acta1862(5)474-484(2017 05)

N-Acylethanolamine-hydrolyzing acid amidase inhibition increases colon N-palmitoylethanolamine levels and counteracts murine colitis.
Alhouayek M, Bottemanne P, Subramanian KV, Lambert DM, Makriyannis A, Cani PD, Muccioli GG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(2)650-61(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both inhibitors reduced several markers of macrophage activation, such as mRNA expression of inflammatory mediators, as well as cytokine and prostaglandin production, with however some differences between FAAH and NAAA inhibition. Our results support an important role for inhibition of NAE hydrolysis and NAAA inhibition in particular in controlling macrophage activation, and thus inflammation.
Title: N-acylethanolamine-hydrolyzing acid amidase and fatty acid amide hydrolase inhibition differentially affect N-acylethanolamine levels and macrophage activation.

NAAA inhibition increases palmitoylethanolamide levels in the colon and reduces colon inflammation and systemic inflammation
Title: N-Acylethanolamine-hydrolyzing acid amidase inhibition increases colon N-palmitoylethanolamine levels and counteracts murine colitis.

NAAA regulates peripheral pain initiation by interrupting endogenous fatty acid ethanolamide signaling at PPAR-alpha.
Title: Antinociceptive effects of the N-acylethanolamine acid amidase inhibitor ARN077 in rodent pain models.

Results suggest that N-acylethanolamine-hydrolyzing acid amidase and fatty acid amide hydrolase cooperatively degrade anandamide and other N-acylethanolamines in macrophages.
Title: Involvement of N-acylethanolamine-hydrolyzing acid amidase in the degradation of anandamide and other N-acylethanolamines in macrophages.

The following Naaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Naaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13664	NM_001163687.1 Latest version!	Mus musculus N-acylethanolamine acid amidase (Naaa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu13979	NM_025972.4 Latest version!	Mus musculus N-acylethanolamine acid amidase (Naaa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Naaa ( NM_001163687.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157159.1 Mus musculus N-acylethanolamine acid amidase (Naaa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13664
Clone ID Related Accession (Same CDS sequence)	NM_001163687.1
Accession Version	NM_001163687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	N-acylethanolamine-hydrolyzing acid amidase isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008776.1, AK017141.1, AC125542.4 and AK133485.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter isoform (2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK017141.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCC TAGCCACCAG GGCCGCGTGC CATGGGGCAC ACCTGGCCCT GGCTCTGTTG 
CTGCTACTGT CGCTGTCCGG TCCCTGGCTG TCGGCTGTCG TCCCCGGGAC TCCGCCTCTC 
TTCAACGTCA GTCTGGACGC GGCCCCGGAG CAGCGCTGGC TGCCGATGCT GCGGCACTAC 
GACCCAGACT TCTTGCGCAC CGCGGTGGCG CAGGTCATTG GGGTTCCCCA GTGGGTACTC 
GGGATGGTCG GAGAAATTGT GTCGAAGGTG GAGAGCTTCC TGCCTCAGCC CTTCACCGAC 
GAGATCCGCA GCATCTGTGA CTCGCTCAAC CTCAGCCTGG CTGACGGTAT CTTGGTCAAC 
CTGGCCTACG AGGCTTCCGC ATTCTGCACC AGTATTGTGG CCCAAGACTC CCAAGGCCAT 
ATTTACCACG GCCGGAACCT GGACTATCCT TTTGGAAAAA TCTTACGAAA GCTGACAGCG 
AACGTGCAGT TTATAAAGAA TGGGCAGATT GCATTCACTG GGACCACTTT TGTTGGCTAT 
GTAGGACTGT GGACAGGCCA AAGTCCACAC AAGTTTACAA TTTCTGGTGA TGAACGAGAT 
AAAGGCTGGT GGTGGGAGAA TATGATCGCG GCGCTTTCTC TGGGACACTC CCCCATCAGC 
TGGCTTATCC GCAAAACCCT GAGTGAGTCA GAAAGCTTCG AAGCAGCTGT CTACACGCTG 
GCCAAGACTC CCCTCATTGC TGACGTTTAT TACATTGTTG GGGGTACATC GCCCAAGGAG 
GGAGTAGTCA TCACCAGGGA CCGAGGTGGC CCGGCAGACA TTTGGCCTCT TGACCCTCTG 
AATGGAGAGT GGTTCCGAGT TGAGACAAAT TATGACCATT GGAAGCCTGC ACCCAAGGTG 
GATGACCGAA GAACACCAGC CATCAAAGCC CTAAATGCTA CAGGGCAAGC TCACCTCAAT 
CTGGAGACCC TCTTCCAGGT TTTATCCCTG TTTCCTGTTT ATAACAACTA CACAATTTAT 
ACTACAGTGA TGAGCGCTGC CGAGCCCGAC AAGTACCTGA CCATGATCAG AAACCCGAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157159.1
CDS	50..1132
Translation	MGTLATRAACHGAHLALALLLLLSLSGPWLSAVVPGTPPLFNVSLDAAPEQRWLPMLRHYDPDFLRTAVAQVIGVPQWVLGMVGEIVSKVESFLPQPFTDEIRSICDSLNLSLADGILVNLAYEASAFCTSIVAQDSQGHIYHGRNLDYPFGKILRKLTANVQFIKNGQIAFTGTTFVGYVGLWTGQSPHKFTISGDERDKGWWWENMIAALSLGHSPISWLIRKTLSESESFEAAVYTLAKTPLIADVYYIVGGTSPKEGVVITRDRGGPADIWPLDPLNGEWFRVETNYDHWKPAPKVDDRRTPAIKALNATGQAHLNLETLFQVLSLFPVYNNYTIYTTVMSAAEPDKYLTMIRNPS

Target ORF information:

RefSeq Version	NM_001163687.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylethanolamine acid amidase (Naaa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163687.1

ORF Insert Sequence:

ATGGGGACCC TAGCCACCAG GGCCGCGTGC CATGGGGCAC ACCTGGCCCT GGCTCTGTTG 
CTGCTACTGT CGCTGTCCGG TCCCTGGCTG TCGGCTGTCG TCCCCGGGAC TCCGCCTCTC 
TTCAACGTCA GTCTGGACGC GGCCCCGGAG CAGCGCTGGC TGCCGATGCT GCGGCACTAC 
GACCCAGACT TCTTGCGCAC CGCGGTGGCG CAGGTCATTG GGGTTCCCCA GTGGGTACTC 
GGGATGGTCG GAGAAATTGT GTCGAAGGTG GAGAGCTTCC TGCCTCAGCC CTTCACCGAC 
GAGATCCGCA GCATCTGTGA CTCGCTCAAC CTCAGCCTGG CTGACGGTAT CTTGGTCAAC 
CTGGCCTACG AGGCTTCCGC ATTCTGCACC AGTATTGTGG CCCAAGACTC CCAAGGCCAT 
ATTTACCACG GCCGGAACCT GGACTATCCT TTTGGAAAAA TCTTACGAAA GCTGACAGCG 
AACGTGCAGT TTATAAAGAA TGGGCAGATT GCATTCACTG GGACCACTTT TGTTGGCTAT 
GTAGGACTGT GGACAGGCCA AAGTCCACAC AAGTTTACAA TTTCTGGTGA TGAACGAGAT 
AAAGGCTGGT GGTGGGAGAA TATGATCGCG GCGCTTTCTC TGGGACACTC CCCCATCAGC 
TGGCTTATCC GCAAAACCCT GAGTGAGTCA GAAAGCTTCG AAGCAGCTGT CTACACGCTG 
GCCAAGACTC CCCTCATTGC TGACGTTTAT TACATTGTTG GGGGTACATC GCCCAAGGAG 
GGAGTAGTCA TCACCAGGGA CCGAGGTGGC CCGGCAGACA TTTGGCCTCT TGACCCTCTG 
AATGGAGAGT GGTTCCGAGT TGAGACAAAT TATGACCATT GGAAGCCTGC ACCCAAGGTG 
GATGACCGAA GAACACCAGC CATCAAAGCC CTAAATGCTA CAGGGCAAGC TCACCTCAAT 
CTGGAGACCC TCTTCCAGGT TTTATCCCTG TTTCCTGTTT ATAACAACTA CACAATTTAT 
ACTACAGTGA TGAGCGCTGC CGAGCCCGAC AAGTACCTGA CCATGATCAG AAACCCGAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Naaa ( NM_025972.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080248.2 Mus musculus N-acylethanolamine acid amidase (Naaa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13979
Clone ID Related Accession (Same CDS sequence)	NM_025972.4
Accession Version	NM_025972.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	N-acylethanolamine-hydrolyzing acid amidase isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008776.1, AB162194.1, AK133485.1 and AC125542.4. On Aug 8, 2009 this sequence version replaced NM_025972.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK133485.1, SRR1660817.135587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCC TAGCCACCAG GGCCGCGTGC CATGGGGCAC ACCTGGCCCT GGCTCTGTTG 
CTGCTACTGT CGCTGTCCGG TCCCTGGCTG TCGGCTGTCG TCCCCGGGAC TCCGCCTCTC 
TTCAACGTCA GTCTGGACGC GGCCCCGGAG CAGCGCTGGC TGCCGATGCT GCGGCACTAC 
GACCCAGACT TCTTGCGCAC CGCGGTGGCG CAGGTCATTG GCGACAGGGT TCCCCAGTGG 
GTACTCGGGA TGGTCGGAGA AATTGTGTCG AAGGTGGAGA GCTTCCTGCC TCAGCCCTTC 
ACCGACGAGA TCCGCAGCAT CTGTGACTCG CTCAACCTCA GCCTGGCTGA CGGTATCTTG 
GTCAACCTGG CCTACGAGGC TTCCGCATTC TGCACCAGTA TTGTGGCCCA AGACTCCCAA 
GGCCATATTT ACCACGGCCG GAACCTGGAC TATCCTTTTG GAAAAATCTT ACGAAAGCTG 
ACAGCGAACG TGCAGTTTAT AAAGAATGGG CAGATTGCAT TCACTGGGAC CACTTTTGTT 
GGCTATGTAG GACTGTGGAC AGGCCAAAGT CCACACAAGT TTACAATTTC TGGTGATGAA 
CGAGATAAAG GCTGGTGGTG GGAGAATATG ATCGCGGCGC TTTCTCTGGG ACACTCCCCC 
ATCAGCTGGC TTATCCGCAA AACCCTGAGT GAGTCAGAAA GCTTCGAAGC AGCTGTCTAC 
ACGCTGGCCA AGACTCCCCT CATTGCTGAC GTTTATTACA TTGTTGGGGG TACATCGCCC 
AAGGAGGGAG TAGTCATCAC CAGGGACCGA GGTGGCCCGG CAGACATTTG GCCTCTTGAC 
CCTCTGAATG GAGAGTGGTT CCGAGTTGAG ACAAATTATG ACCATTGGAA GCCTGCACCC 
AAGGTGGATG ACCGAAGAAC ACCAGCCATC AAAGCCCTAA ATGCTACAGG GCAAGCTCAC 
CTCAATCTGG AGACCCTCTT CCAGGTTTTA TCCCTGTTTC CTGTTTATAA CAACTACACA 
ATTTATACTA CAGTGATGAG CGCTGCCGAG CCCGACAAGT ACCTGACCAT GATCAGAAAC 
CCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080248.2
CDS	50..1138
Translation	MGTLATRAACHGAHLALALLLLLSLSGPWLSAVVPGTPPLFNVSLDAAPEQRWLPMLRHYDPDFLRTAVAQVIGDRVPQWVLGMVGEIVSKVESFLPQPFTDEIRSICDSLNLSLADGILVNLAYEASAFCTSIVAQDSQGHIYHGRNLDYPFGKILRKLTANVQFIKNGQIAFTGTTFVGYVGLWTGQSPHKFTISGDERDKGWWWENMIAALSLGHSPISWLIRKTLSESESFEAAVYTLAKTPLIADVYYIVGGTSPKEGVVITRDRGGPADIWPLDPLNGEWFRVETNYDHWKPAPKVDDRRTPAIKALNATGQAHLNLETLFQVLSLFPVYNNYTIYTTVMSAAEPDKYLTMIRNPS

Target ORF information:

RefSeq Version	NM_025972.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acylethanolamine acid amidase (Naaa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025972.4

ORF Insert Sequence:

ATGGGGACCC TAGCCACCAG GGCCGCGTGC CATGGGGCAC ACCTGGCCCT GGCTCTGTTG 
CTGCTACTGT CGCTGTCCGG TCCCTGGCTG TCGGCTGTCG TCCCCGGGAC TCCGCCTCTC 
TTCAACGTCA GTCTGGACGC GGCCCCGGAG CAGCGCTGGC TGCCGATGCT GCGGCACTAC 
GACCCAGACT TCTTGCGCAC CGCGGTGGCG CAGGTCATTG GCGACAGGGT TCCCCAGTGG 
GTACTCGGGA TGGTCGGAGA AATTGTGTCG AAGGTGGAGA GCTTCCTGCC TCAGCCCTTC 
ACCGACGAGA TCCGCAGCAT CTGTGACTCG CTCAACCTCA GCCTGGCTGA CGGTATCTTG 
GTCAACCTGG CCTACGAGGC TTCCGCATTC TGCACCAGTA TTGTGGCCCA AGACTCCCAA 
GGCCATATTT ACCACGGCCG GAACCTGGAC TATCCTTTTG GAAAAATCTT ACGAAAGCTG 
ACAGCGAACG TGCAGTTTAT AAAGAATGGG CAGATTGCAT TCACTGGGAC CACTTTTGTT 
GGCTATGTAG GACTGTGGAC AGGCCAAAGT CCACACAAGT TTACAATTTC TGGTGATGAA 
CGAGATAAAG GCTGGTGGTG GGAGAATATG ATCGCGGCGC TTTCTCTGGG ACACTCCCCC 
ATCAGCTGGC TTATCCGCAA AACCCTGAGT GAGTCAGAAA GCTTCGAAGC AGCTGTCTAC 
ACGCTGGCCA AGACTCCCCT CATTGCTGAC GTTTATTACA TTGTTGGGGG TACATCGCCC 
AAGGAGGGAG TAGTCATCAC CAGGGACCGA GGTGGCCCGG CAGACATTTG GCCTCTTGAC 
CCTCTGAATG GAGAGTGGTT CCGAGTTGAG ACAAATTATG ACCATTGGAA GCCTGCACCC 
AAGGTGGATG ACCGAAGAAC ACCAGCCATC AAAGCCCTAA ATGCTACAGG GCAAGCTCAC 
CTCAATCTGG AGACCCTCTT CCAGGTTTTA TCCCTGTTTC CTGTTTATAA CAACTACACA 
ATTTATACTA CAGTGATGAG CGCTGCCGAG CCCGACAAGT ACCTGACCAT GATCAGAAAC 
CCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The N-acylethanolamine acid amidase inhibitor ARN077 suppresses inflammation and pruritus in a mouse model of allergic dermatitis.
The Journal of investigative dermatology(2017 Oct)
Sasso O,Summa M,Armirotti A,Pontis S,De Mei C,Piomelli D

N-acylethanolamine-hydrolyzing acid amidase and fatty acid amide hydrolase inhibition differentially affect N-acylethanolamine levels and macrophage activation.
Biochimica et biophysica acta1862(5)474-484(2017 05)
Alhouayek M,Bottemanne P,Makriyannis A,Muccioli GG

N-Acylethanolamine-hydrolyzing acid amidase inhibition increases colon N-palmitoylethanolamine levels and counteracts murine colitis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(2)650-61(2015 Feb)
Alhouayek M,Bottemanne P,Subramanian KV,Lambert DM,Makriyannis A,Cani PD,Muccioli GG

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Naaa cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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