Ndc80 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndc80
Entrez Gene ID	67052
Full Name	NDC80 kinetochore complex component
Synonyms	2610020P18Rik,AV139730,HEC,HEC1,Kntc2
General protein information	Preferred Names NDC80 kinetochore complex component Names kinetochore protein NDC80 homolog NDC80 homolog, kinetochore complex component highly expressed in cancer, rich in leucine heptad repeats kinetochore associated 2 kinetochore protein Hec1 kinetochore-associated protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_023294.2	NP_075783.2	kinetochore protein NDC80 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NDC80	XP_001146732.1
Bos taurus (cattle)	NDC80	NP_001193512.1
Mus musculus (house mouse)	Ndc80	NP_075783.2
	NDC80	XP_001086615.1
Gallus gallus (chicken)	NDC80	NP_989808.2
Danio rerio (zebrafish)	ndc80	NP_001003863.1
Canis lupus familiaris (dog)	NDC80	XP_537313.2
Oryza sativa (rice)	Os08g0468400	NP_001062008.1
Xenopus tropicalis (tropical clawed frog)	ndc80	NP_001011162.1
Arabidopsis thaliana (thale cress)	AT3G54630	NP_191024.1
Homo sapiens (human)	NDC80	NP_006092.1
Rattus norvegicus (Norway rat)	Ndc80	NP_001119742.1

Gene Ontology
Bibliography

Related articles in PubMed

Anti-tumor activity of KNTC2 siRNA in orthotopic tumor model mice of hepatocellular carcinoma.
Makita Y, Murata S, Katou Y, Kikuchi K, Uejima H, Teratani M, Hoashi Y, Kenjo E, Matsumoto S, Nogami M, Otake K, Kawamata Y
Biochemical and biophysical research communications493(1)800-806(2017 11)

Kif4 Is Essential for Mouse Oocyte Meiosis.
Camlin NJ, McLaughlin EA, Holt JE
PloS one12(1)e0170650(2017)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

Hec1-dependent cyclin B2 stabilization regulates the G2-M transition and early prometaphase in mouse oocytes.
Gui L, Homer H
Developmental cell25(1)43-54(2013 Apr)

Ndc80 regulates meiotic spindle organization, chromosome alignment, and cell cycle progression in mouse oocytes.
Sun SC, Zhang DX, Lee SE, Xu YN, Kim NH
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada17(3)431-9(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data strongly indicated that KNTC2 is a promising target for the treatment of HCC and that phosphorylated HH3 at serine 10 is one of the target engagement markers for KNTC2.
Title: Anti-tumor activity of KNTC2 siRNA in orthotopic tumor model mice of hepatocellular carcinoma.

Further investigation of the phosphoregulation of meiotic Kif4 revealed that Aurora Kinase and Cdk activity is critical for Kif4 kinetochore localization and interaction with Ndc80 and CENP-C. Finally, Kif4 protein but not gene expression was found to be upregulated with age, suggesting a role for this protein in the decline of oocyte quality with age
Title: Kif4 Is Essential for Mouse Oocyte Meiosis.

Hec1-dependent cyclin B2 stabilization during early metaphase is important for early-stage spindle assembly.
Title: Hec1-dependent cyclin B2 stabilization regulates the G2-M transition and early prometaphase in mouse oocytes.

Disruption of Ndc80 function by either siRNA injection or antibody injection resulted in severe chromosome misalignment, spindle disruption, and precocious polar body extrusion.
Title: Ndc80 regulates meiotic spindle organization, chromosome alignment, and cell cycle progression in mouse oocytes.

overexpression of Hec1 results in mitotic checkpoint hyperactivation that leads to aneuploidy sufficient to generate tumors
Title: Hec1 overexpression hyperactivates the mitotic checkpoint and induces tumor formation in vivo.

The following Ndc80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndc80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01304	NM_023294.2 Latest version!	Mus musculus NDC80 kinetochore complex component (Ndc80), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$377.30 $539.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndc80 ( NM_023294.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075783.2 Mus musculus NDC80 kinetochore complex component (Ndc80), mRNA.

Price & Availability↑

CloneID	OMu01304
Clone ID Related Accession (Same CDS sequence)	NM_023294.2
Accession Version	NM_023294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	kinetochore protein NDC80 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF326730.1, BX527376.1, AK011497.1 and CJ201671.1. On Dec 10, 2008 this sequence version replaced NM_023294.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF326730.1, AK011497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCGCA GTTCAGTTTC CACCTGTGGT GCTGGCCGCC TCTCTATGCA GGAGTTAAGG 
ACCCTGGACC TCAATAAGCC AGGCCTTTAT ACCCCTCAAA CCAAAGAAAG ATCAACCTTT 
GGAAAGCTGA GTACACACAA ACCGACATCG GAAAGAAAAG TCTCAATATT TGGGAAAAGG 
ACTAGCGGAC ATGGATCCAG GAATAGTCAA CTTGGTATAT TTTCCAGTTC TGAAAAAATC 
AAGGACCCAA GACCACTTAA TGACAAAGCA TTCATTCAGC AGTGTATTCG ACAACTCTAT 
GAGTTTCTTA CAGAAAACGG TTATGTGTAT AGTGTATCCA TGAAGTCTCT GCAAGCTCCA 
TCCACTAAAG AGTTCCTAAA GATCTTCGCC TTTCTTTATG GCTTTCTGTG CCCGTCGTAT 
GAACTTCCTG GTACAAAATG TGAAGAAGAG GTCCCAAGAA TTTTTAAAGC ACTTGGGTAT 
CCCTTCACAC TGTCCAAGAG CTCCATGTAT ACAGTGGGAG CCCCTCACAC GTGGCCTCAC 
ATCGTGGCTG CCTTGGTGTG GCTCATAGAC TGCATCAAGA TTGATACTGC CATGAAAGAA 
AGCTCACCTT TATTTGATGA TGGGCAGCTC TGGGGAGAAG AGACTGAAGA TGGAATTAAA 
CACAATAAGT TGTTTTTGGA GTACACCAAA AAGTGCTATG AGAAGTTCAT GACCGGGGCC 
GACAGCTTTG AAGAAGAGGA TGCTGAGCTG CAGGCGAAGC TGAAGGACTT GTACAAGGTA 
GATGCGTCTA AGCTGGAGTC ACTCGAAGCA GAAAACAAAG CACTAAATGA ACAGATTGCA 
AGACTGGAGG AGGAAAGAGA AAGAGAACCG AACCGTCTGA TGTCATTGAA GAAACTGAAA 
GCGTCCTTAC AAGCAGATGT TCAAAACTAT AAAGCATACA TGAGCAACTT GGAGTCTCAT 
TTAGCCGTCC TGAAACAGAA ATCGAATAGT CTTGATGAAG AAATTGGTAG AGTAGAACAA 
GAATGTGAAA CTGTTAAACA GGAAAACACT CGACTACAGA GTATCGTTGA TAACCAGAAG 
TATTCAGTCG CTGACATTGA GAGAATAAAT CATGAGAAAA ATGAATTGCA GCAGACTATT 
AATAAATTAA CCAAAGACCT GGAAGCCGAA CAGCAACAGA TGTGGAATGA AGAATTAAAA 
TACGCAAGAG GCAAAGAGGC GATTGAAGCG CAGCTAGCGG AGTACCACAA GTTGGCTAGA 
AAATTAAAGC TTATCCCCAA AGGTGCTGAG AATTCCAAAG GTTACGACTT TGAAATTAAG 
TTTAATCCTG AGGCGGGTGC CAACTGCCTT GTCAAATACA GGACTCAAGT GTATGCACCG 
CTCAAAGAGC TCTTGAATGA AAGCGAAGAA GAAATTAACA AAGCTCTGAA TAAAAAGAGG 
CATCTGGAGG ATACTTTAGA ACAACTGAAC ACCATGAAAA CGGAAAGCAA GAACACTGTG 
AGGATGCTGA AGGAGGAGAT TCAGAAACTG GATGACCTTC ACCAGCAGGC AGTGAAGGAA 
GCTGAGGAAA AAGACAAGAA GAGTGCCAGT GAGCTTGAGT CCCTGGAGAA ACACAAGCAC 
CTGCTGGAGA GCGGGGTGAA CGATGGCCTC AGCGAGGCCA TGGATGAGTT GGACGCCGTC 
CAGCGGGAAT ACCAGCTAAC TGTGAAGACC ACAACTGAAG AAAGAAGAAA GGTGGAAAAC 
AACTTACAAC GTCTTTTGGA GATGGTCGCC ACACACGTAG GGTCTTTGGA GAAACATCTT 
GAAGAGGAGA ATGCTAAAGC CGACAGAGAG TATGAAGAAT TCATGTCTGA AGATCTCCTG 
GAAAACATCA GGGAGATGGC AGAGAAGTAT AAGAGAAATG CTGCCCAACT TAAGGCTCCC 
GACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075783.2
CDS	157..2085
Translation	MKRSSVSTCGAGRLSMQELRTLDLNKPGLYTPQTKERSTFGKLSTHKPTSERKVSIFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLYEFLTENGYVYSVSMKSLQAPSTKEFLKIFAFLYGFLCPSYELPGTKCEEEVPRIFKALGYPFTLSKSSMYTVGAPHTWPHIVAALVWLIDCIKIDTAMKESSPLFDDGQLWGEETEDGIKHNKLFLEYTKKCYEKFMTGADSFEEEDAELQAKLKDLYKVDASKLESLEAENKALNEQIARLEEEREREPNRLMSLKKLKASLQADVQNYKAYMSNLESHLAVLKQKSNSLDEEIGRVEQECETVKQENTRLQSIVDNQKYSVADIERINHEKNELQQTINKLTKDLEAEQQQMWNEELKYARGKEAIEAQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRTQVYAPLKELLNESEEEINKALNKKRHLEDTLEQLNTMKTESKNTVRMLKEEIQKLDDLHQQAVKEAEEKDKKSASELESLEKHKHLLESGVNDGLSEAMDELDAVQREYQLTVKTTTEERRKVENNLQRLLEMVATHVGSLEKHLEEENAKADREYEEFMSEDLLENIREMAEKYKRNAAQLKAPDK

Target ORF information:

RefSeq Version	NM_023294.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NDC80 kinetochore complex component (Ndc80), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023294.2

ORF Insert Sequence:

ATGAAGCGCA GTTCAGTTTC CACCTGTGGT GCTGGCCGCC TCTCTATGCA GGAGTTAAGG 
ACCCTGGACC TCAATAAGCC AGGCCTTTAT ACCCCTCAAA CCAAAGAAAG ATCAACCTTT 
GGAAAGCTGA GTACACACAA ACCGACATCG GAAAGAAAAG TCTCAATATT TGGGAAAAGG 
ACTAGCGGAC ATGGATCCAG GAATAGTCAA CTTGGTATAT TTTCCAGTTC TGAAAAAATC 
AAGGACCCAA GACCACTTAA TGACAAAGCA TTCATTCAGC AGTGTATTCG ACAACTCTAT 
GAGTTTCTTA CAGAAAACGG TTATGTGTAT AGTGTATCCA TGAAGTCTCT GCAAGCTCCA 
TCCACTAAAG AGTTCCTAAA GATCTTCGCC TTTCTTTATG GCTTTCTGTG CCCGTCGTAT 
GAACTTCCTG GTACAAAATG TGAAGAAGAG GTCCCAAGAA TTTTTAAAGC ACTTGGGTAT 
CCCTTCACAC TGTCCAAGAG CTCCATGTAT ACAGTGGGAG CCCCTCACAC GTGGCCTCAC 
ATCGTGGCTG CCTTGGTGTG GCTCATAGAC TGCATCAAGA TTGATACTGC CATGAAAGAA 
AGCTCACCTT TATTTGATGA TGGGCAGCTC TGGGGAGAAG AGACTGAAGA TGGAATTAAA 
CACAATAAGT TGTTTTTGGA GTACACCAAA AAGTGCTATG AGAAGTTCAT GACCGGGGCC 
GACAGCTTTG AAGAAGAGGA TGCTGAGCTG CAGGCGAAGC TGAAGGACTT GTACAAGGTA 
GATGCGTCTA AGCTGGAGTC ACTCGAAGCA GAAAACAAAG CACTAAATGA ACAGATTGCA 
AGACTGGAGG AGGAAAGAGA AAGAGAACCG AACCGTCTGA TGTCATTGAA GAAACTGAAA 
GCGTCCTTAC AAGCAGATGT TCAAAACTAT AAAGCATACA TGAGCAACTT GGAGTCTCAT 
TTAGCCGTCC TGAAACAGAA ATCGAATAGT CTTGATGAAG AAATTGGTAG AGTAGAACAA 
GAATGTGAAA CTGTTAAACA GGAAAACACT CGACTACAGA GTATCGTTGA TAACCAGAAG 
TATTCAGTCG CTGACATTGA GAGAATAAAT CATGAGAAAA ATGAATTGCA GCAGACTATT 
AATAAATTAA CCAAAGACCT GGAAGCCGAA CAGCAACAGA TGTGGAATGA AGAATTAAAA 
TACGCAAGAG GCAAAGAGGC GATTGAAGCG CAGCTAGCGG AGTACCACAA GTTGGCTAGA 
AAATTAAAGC TTATCCCCAA AGGTGCTGAG AATTCCAAAG GTTACGACTT TGAAATTAAG 
TTTAATCCTG AGGCGGGTGC CAACTGCCTT GTCAAATACA GGACTCAAGT GTATGCACCG 
CTCAAAGAGC TCTTGAATGA AAGCGAAGAA GAAATTAACA AAGCTCTGAA TAAAAAGAGG 
CATCTGGAGG ATACTTTAGA ACAACTGAAC ACCATGAAAA CGGAAAGCAA GAACACTGTG 
AGGATGCTGA AGGAGGAGAT TCAGAAACTG GATGACCTTC ACCAGCAGGC AGTGAAGGAA 
GCTGAGGAAA AAGACAAGAA GAGTGCCAGT GAGCTTGAGT CCCTGGAGAA ACACAAGCAC 
CTGCTGGAGA GCGGGGTGAA CGATGGCCTC AGCGAGGCCA TGGATGAGTT GGACGCCGTC 
CAGCGGGAAT ACCAGCTAAC TGTGAAGACC ACAACTGAAG AAAGAAGAAA GGTGGAAAAC 
AACTTACAAC GTCTTTTGGA GATGGTCGCC ACACACGTAG GGTCTTTGGA GAAACATCTT 
GAAGAGGAGA ATGCTAAAGC CGACAGAGAG TATGAAGAAT TCATGTCTGA AGATCTCCTG 
GAAAACATCA GGGAGATGGC AGAGAAGTAT AAGAGAAATG CTGCCCAACT TAAGGCTCCC 
GACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Anti-tumor activity of KNTC2 siRNA in orthotopic tumor model mice of hepatocellular carcinoma.
Biochemical and biophysical research communications493(1)800-806(2017 11)
Makita Y,Murata S,Katou Y,Kikuchi K,Uejima H,Teratani M,Hoashi Y,Kenjo E,Matsumoto S,Nogami M,Otake K,Kawamata Y

Kif4 Is Essential for Mouse Oocyte Meiosis.
PloS one12(1)e0170650(2017)
Camlin NJ,McLaughlin EA,Holt JE

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

Hec1-dependent cyclin B2 stabilization regulates the G2-M transition and early prometaphase in mouse oocytes.
Developmental cell25(1)43-54(2013 Apr)
Gui L,Homer H

Ndc80 regulates meiotic spindle organization, chromosome alignment, and cell cycle progression in mouse oocytes.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada17(3)431-9(2011 Jun)
Sun SC,Zhang DX,Lee SE,Xu YN,Kim NH

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Ndc80 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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