Mcub cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcub
Entrez Gene ID	66815
Full Name	mitochondrial calcium uniporter dominant negative beta subunit
Synonyms	9030408N13Rik,Ccdc109b
General protein information	Preferred Names mitochondrial calcium uniporter dominant negative beta subunit Names calcium uniporter regulatory subunit MCUb, mitochondrial coiled-coil domain containing 109B coiled-coil domain-containing protein 109B mitochondrial calcium uniporter regulatory subunit MCUb
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501900.1	XP_006501963.1	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X1
XM_006501901.3	XP_006501964.1	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X2
NM_025779.3	NP_080055.2	calcium uniporter regulatory subunit MCUb, mitochondrial isoform1
XM_006501900.2	XP_006501963.1	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X1
XM_006501901.4	XP_006501964.1	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X2
XM_011240197.2	XP_011238499.1	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X3
NM_001293644.1	NP_001280573.1	calcium uniporter regulatory subunit MCUb, mitochondrial isoform 2
XM_011240197.3	XP_011238499.1	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ccdc109b	NP_001120048.1
Homo sapiens (human)	CCDC109B	NP_060388.2
Pan troglodytes (chimpanzee)	CCDC109B	XP_517392.2
Mus musculus (house mouse)	Ccdc109b	NP_080055.2
Rattus norvegicus (Norway rat)	Ccdc109b	XP_006233342.1
Gallus gallus (chicken)	CCDC109B	XP_420651.4
	LOC698670	XP_001087164.1
Canis lupus familiaris (dog)	CCDC109B	XP_005639324.1
Bos taurus (cattle)	CCDC109B	NP_001068639.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Expression of mRNA Encoding Mcu and Other Mitochondrial Calcium Regulatory Genes Depends on Cell Type, Neuronal Subtype, and Ca2+ Signaling.
M?rkus NM, Hasel P, Qiu J, Bell KF, Heron S, Kind PC, Dando O, Simpson TI, Hardingham GE
PloS one11(2)e0148164(2016)

The mitochondrial calcium uniporter is a multimer that can include a dominant-negative pore-forming subunit.
Raffaello A, De Stefani D, Sabbadin D, Teardo E, Merli G, Picard A, Checchetto V, Moro S, Szab? I, Rizzuto R
The EMBO journal32(17)2362-76(2013 Aug)

Brn3a and Nurr1 mediate a gene regulatory pathway for habenula development.
Quina LA, Wang S, Ng L, Turner EE
The Journal of neuroscience : the official journal of the Society for Neuroscience29(45)14309-22(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Functional characterization of the MCUb protein; shows that it inhibits mitochondrial calcium uptake
Title: The mitochondrial calcium uniporter is a multimer that can include a dominant-negative pore-forming subunit.

The following Mcub gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcub cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23422	NM_025779.3 Latest version!	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$237.30 $339.00
OMu23436	NM_001293644.1 Latest version!	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu32179	XM_006501900.2 Latest version!	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu32180	XM_006501901.4 Latest version!	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu70013	XM_011240197.3 Latest version!	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu32179	XM_006501900.1	Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu32180	XM_006501901.3	Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu70013	XM_011240197.2	Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcub ( XM_006501900.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501963.1 Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu32179
Clone ID Related Accession (Same CDS sequence)	XM_006501900.1 , XM_006501900.2
Accession Version	XM_006501900.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGAAGAGCC AAGTGGAGAG CATATGACTG AGTTGGAAAA CACCAAGTCC 
TTGGTTCACA GACTATTCAC TATCCTGCAT TTAGAGGAGA TCCAGAAGAG ACGAGAGCGC 
CATCTGATGG CGAAGATCGA CCACCTGCAG GAACAGCTGC GGCCCTTGGA GCAGGTCAAA 
GCTGCAATAG AAGCCCGGTC GGAAGCCAAC ACCAGTGGTC TCCTGTGGGC CGGCTTAGCT 
CTGCTCTCCG TGCAGGGCGG GGCGCTGGCC TGGCTTACTT GGTGGGTGTA CTCCTGGGAT 
ATCATGGAGC CAGTTACCTT CTTCCTGTCG TTTGCGAATT CCATCGTCTT TTTTGCATAC 
TTCATCATAA CCCGGCAGAA CTACACATAC TCATCCCTCC GGAGCAGGCA GTTTCTTCAG 
TTCTTCCACA AGAAATCGCA GCGGCGGTGT TTTGATGTGG AACAATACAA CAAGCTAAAG 
GAAGACCTTG CTGAGGCTAC AGAATCCCTG GAAAGCGTGC GCCGCTCCCT CCGCTTGCGA 
ATCCAAGGAG AGGAAGCCAG CGAGAAGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501963.1
CDS	140..1072
Translation	MPGALSGRRMLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPKYHQALHYGTVEPQDEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITAEEPSGEHMTELENTKSLVHRLFTILHLEEIQKRRERHLMAKIDHLQEQLRPLEQVKAAIEARSEANTSGLLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLSFANSIVFFAYFIITRQNYTYSSLRSRQFLQFFHKKSQRRCFDVEQYNKLKEDLAEATESLESVRRSLRLRIQGEEASEKN

Target ORF information:

RefSeq Version	XM_006501900.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501900.1

ORF Insert Sequence:

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGAAGAGCC AAGTGGAGAG CATATGACTG AGTTGGAAAA CACCAAGTCC 
TTGGTTCACA GACTATTCAC TATCCTGCAT TTAGAGGAGA TCCAGAAGAG ACGAGAGCGC 
CATCTGATGG CGAAGATCGA CCACCTGCAG GAACAGCTGC GGCCCTTGGA GCAGGTCAAA 
GCTGCAATAG AAGCCCGGTC GGAAGCCAAC ACCAGTGGTC TCCTGTGGGC CGGCTTAGCT 
CTGCTCTCCG TGCAGGGCGG GGCGCTGGCC TGGCTTACTT GGTGGGTGTA CTCCTGGGAT 
ATCATGGAGC CAGTTACCTT CTTCCTGTCG TTTGCGAATT CCATCGTCTT TTTTGCATAC 
TTCATCATAA CCCGGCAGAA CTACACATAC TCATCCCTCC GGAGCAGGCA GTTTCTTCAG 
TTCTTCCACA AGAAATCGCA GCGGCGGTGT TTTGATGTGG AACAATACAA CAAGCTAAAG 
GAAGACCTTG CTGAGGCTAC AGAATCCCTG GAAAGCGTGC GCCGCTCCCT CCGCTTGCGA 
ATCCAAGGAG AGGAAGCCAG CGAGAAGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcub ( XM_006501901.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501964.1 Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32180
Clone ID Related Accession (Same CDS sequence)	XM_006501901.3 , XM_006501901.4
Accession Version	XM_006501901.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGT CGGTGGCAGC TGCTGCGGAC GATCCGAGCC CGCGGCCGCG GGGATCCCAG 
GGAGCTGCCA TCCACACCCC AGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGATGGCAG TGAAATTCCA GCTTCAACAT TGATGGACAC TTTGCTAATG 
ACAGATTTTA AGCTCATCAT TAACAAACTA CGGTATGATA TACGGTGTCA CAAGAAAGAA 
GAGCCAAGTG GAGAGCATAT GACTGAGTTG GAAAACACCA AGTCCTTGGT TCACAGACTA 
TTCACTATCC TGCATTTAGA GGAGATCCAG AAGAGACGAG AGCGCCATCT GATGGCGAAG 
ATCGACCACC TGCAGGAACA GCTGCGGCCC TTGGAGCAGG TCAAAGCTGC AATAGAAGCC 
CGGTCGGAAG CCAACACCAG TGGTCTCCTG TGGGCCGGCT TAGCTCTGCT CTCCGTGCAG 
GGCGGGGCGC TGGCCTGGCT TACTTGGTGG GTGTACTCCT GGGATATCAT GGAGCCAGTT 
ACCTTCTTCC TGTCGTTTGC GAATTCCATC GTCTTTTTTG CATACTTCAT CATAACCCGG 
CAGAACTACA CATACTCATC CCTCCGGAGC AGGCAGTTTC TTCAGTTCTT CCACAAGAAA 
TCGCAGCGGC GGTGTTTTGA TGTGGAACAA TACAACAAGC TAAAGGAAGA CCTTGCTGAG 
GCTACAGAAT CCCTGGAAAG CGTGCGCCGC TCCCTCCGCT TGCGAATCCA AGGAGAGGAA 
GCCAGCGAGA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501964.1
CDS	184..1101
Translation	MSGSVAAAADDPSPRPRGSQGAAIHTPEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITADGSEIPASTLMDTLLMTDFKLIINKLRYDIRCHKKEEPSGEHMTELENTKSLVHRLFTILHLEEIQKRRERHLMAKIDHLQEQLRPLEQVKAAIEARSEANTSGLLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLSFANSIVFFAYFIITRQNYTYSSLRSRQFLQFFHKKSQRRCFDVEQYNKLKEDLAEATESLESVRRSLRLRIQGEEASEKN

Target ORF information:

RefSeq Version	XM_006501901.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501901.3

ORF Insert Sequence:

ATGTCTGGGT CGGTGGCAGC TGCTGCGGAC GATCCGAGCC CGCGGCCGCG GGGATCCCAG 
GGAGCTGCCA TCCACACCCC AGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGATGGCAG TGAAATTCCA GCTTCAACAT TGATGGACAC TTTGCTAATG 
ACAGATTTTA AGCTCATCAT TAACAAACTA CGGTATGATA TACGGTGTCA CAAGAAAGAA 
GAGCCAAGTG GAGAGCATAT GACTGAGTTG GAAAACACCA AGTCCTTGGT TCACAGACTA 
TTCACTATCC TGCATTTAGA GGAGATCCAG AAGAGACGAG AGCGCCATCT GATGGCGAAG 
ATCGACCACC TGCAGGAACA GCTGCGGCCC TTGGAGCAGG TCAAAGCTGC AATAGAAGCC 
CGGTCGGAAG CCAACACCAG TGGTCTCCTG TGGGCCGGCT TAGCTCTGCT CTCCGTGCAG 
GGCGGGGCGC TGGCCTGGCT TACTTGGTGG GTGTACTCCT GGGATATCAT GGAGCCAGTT 
ACCTTCTTCC TGTCGTTTGC GAATTCCATC GTCTTTTTTG CATACTTCAT CATAACCCGG 
CAGAACTACA CATACTCATC CCTCCGGAGC AGGCAGTTTC TTCAGTTCTT CCACAAGAAA 
TCGCAGCGGC GGTGTTTTGA TGTGGAACAA TACAACAAGC TAAAGGAAGA CCTTGCTGAG 
GCTACAGAAT CCCTGGAAAG CGTGCGCCGC TCCCTCCGCT TGCGAATCCA AGGAGAGGAA 
GCCAGCGAGA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcub ( XM_011240197.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238499.1 Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu70013
Clone ID Related Accession (Same CDS sequence)	XM_011240197.2 , XM_011240197.3
Accession Version	XM_011240197.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGGTCAAAG CTGCAATAGA AGCCCGGTCG GAAGCCAACA CCAGTGGTCT 
CCTGTGGGCC GGCTTAGCTC TGCTCTCCGT GCAGGGCGGG GCGCTGGCCT GGCTTACTTG 
GTGGGTGTAC TCCTGGGATA TCATGGAGCC AGTTACCTTC TTCCTGTCGT TTGCGAATTC 
CATCGTCTTT TTTGCATACT TCATCATAAC CCGGCAGAAC TACACATACT CATCCCTCCG 
GAGCAGGCAG TTTCTTCAGT TCTTCCACAA GAAATCGCAG CGGCGGTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238499.1
CDS	138..791
Translation	MPGALSGRRMLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPKYHQALHYGTVEPQDEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITAGQSCNRSPVGSQHQWSPVGRLSSALRAGRGAGLAYLVGVLLGYHGASYLLPVVCEFHRLFCILHHNPAELHILIPPEQAVSSVLPQEIAAAVF

Target ORF information:

RefSeq Version	XM_011240197.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus coiled-coil domain containing 109B (Ccdc109b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240197.2

ORF Insert Sequence:

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGGTCAAAG CTGCAATAGA AGCCCGGTCG GAAGCCAACA CCAGTGGTCT 
CCTGTGGGCC GGCTTAGCTC TGCTCTCCGT GCAGGGCGGG GCGCTGGCCT GGCTTACTTG 
GTGGGTGTAC TCCTGGGATA TCATGGAGCC AGTTACCTTC TTCCTGTCGT TTGCGAATTC 
CATCGTCTTT TTTGCATACT TCATCATAAC CCGGCAGAAC TACACATACT CATCCCTCCG 
GAGCAGGCAG TTTCTTCAGT TCTTCCACAA GAAATCGCAG CGGCGGTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcub ( NM_025779.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080055.2 Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23422
Clone ID Related Accession (Same CDS sequence)	NM_025779.3
Accession Version	NM_025779.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Mus musculus(house mouse)
Product	calcium uniporter regulatory subunit MCUb, mitochondrial isoform1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC049571.1 and BX515874.1. On Oct 24, 2007 this sequence version replaced NM_025779.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC049571.1, AK150724.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGATGGCAG TGAAATTCCA GCTTCAACAT TGATGGACAC TTTGCTAATG 
ACAGATTTTA AGCTCATCAT TAACAAACTA CGGTATGATA TACGGTGTCA CAAGAAAGAA 
GAGCCAAGTG GAGAGCATAT GACTGAGTTG GAAAACACCA AGTCCTTGGT TCACAGACTA 
TTCACTATCC TGCATTTAGA GGAGATCCAG AAGAGACGAG AGCGCCATCT GATGGCGAAG 
ATCGACCACC TGCAGGAACA GCTGCGGCCC TTGGAGCAGG TCAAAGCTGC AATAGAAGCC 
CGGTCGGAAG CCAACACCAG TGGTCTCCTG TGGGCCGGCT TAGCTCTGCT CTCCGTGCAG 
GGCGGGGCGC TGGCCTGGCT TACTTGGTGG GTGTACTCCT GGGATATCAT GGAGCCAGTT 
ACCTTCTTCC TGTCGTTTGC GAATTCCATC GTCTTTTTTG CATACTTCAT CATAACCCGG 
CAGAACTACA CATACTCATC CCTCCGGAGC AGGCAGTTTC TTCAGTTCTT CCACAAGAAA 
TCGCAGCGGC GGTGTTTTGA TGTGGAACAA TACAACAAGC TAAAGGAAGA CCTTGCTGAG 
GCTACAGAAT CCCTGGAAAG CGTGCGCCGC TCCCTCCGCT TGCGAATCCA AGGAGAGGAA 
GCCAGCGAGA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080055.2
CDS	132..1169
Translation	MPGALSGRRMLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPKYHQALHYGTVEPQDEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITADGSEIPASTLMDTLLMTDFKLIINKLRYDIRCHKKEEPSGEHMTELENTKSLVHRLFTILHLEEIQKRRERHLMAKIDHLQEQLRPLEQVKAAIEARSEANTSGLLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLSFANSIVFFAYFIITRQNYTYSSLRSRQFLQFFHKKSQRRCFDVEQYNKLKEDLAEATESLESVRRSLRLRIQGEEASEKN

Target ORF information:

RefSeq Version	NM_025779.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025779.3

ORF Insert Sequence:

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGATGGCAG TGAAATTCCA GCTTCAACAT TGATGGACAC TTTGCTAATG 
ACAGATTTTA AGCTCATCAT TAACAAACTA CGGTATGATA TACGGTGTCA CAAGAAAGAA 
GAGCCAAGTG GAGAGCATAT GACTGAGTTG GAAAACACCA AGTCCTTGGT TCACAGACTA 
TTCACTATCC TGCATTTAGA GGAGATCCAG AAGAGACGAG AGCGCCATCT GATGGCGAAG 
ATCGACCACC TGCAGGAACA GCTGCGGCCC TTGGAGCAGG TCAAAGCTGC AATAGAAGCC 
CGGTCGGAAG CCAACACCAG TGGTCTCCTG TGGGCCGGCT TAGCTCTGCT CTCCGTGCAG 
GGCGGGGCGC TGGCCTGGCT TACTTGGTGG GTGTACTCCT GGGATATCAT GGAGCCAGTT 
ACCTTCTTCC TGTCGTTTGC GAATTCCATC GTCTTTTTTG CATACTTCAT CATAACCCGG 
CAGAACTACA CATACTCATC CCTCCGGAGC AGGCAGTTTC TTCAGTTCTT CCACAAGAAA 
TCGCAGCGGC GGTGTTTTGA TGTGGAACAA TACAACAAGC TAAAGGAAGA CCTTGCTGAG 
GCTACAGAAT CCCTGGAAAG CGTGCGCCGC TCCCTCCGCT TGCGAATCCA AGGAGAGGAA 
GCCAGCGAGA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcub ( NM_001293644.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001280573.1 Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant 2, mRNA.

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CloneID	OMu23436
Clone ID Related Accession (Same CDS sequence)	NM_001293644.1
Accession Version	NM_001293644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	calcium uniporter regulatory subunit MCUb, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC049571.1, AK152326.1 and BX515874.1. On Jun 11, 2014 this sequence version replaced XM_006501902.1. Transcript Variant: This variant (2) differs in the 3' UTR and lacks an alternate exon in the 3' coding region resulting in a frameshift compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK152326.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGATGGCAG TGAAATTCCA GCTTCAACAT TGATGGACAC TTTGCTAATG 
ACAGATTTTA AGCTCATCAT TAACAAACTA CGGTATGATA TACGGTGTCA CAAGAAAGGT 
CAAAGCTGCA ATAGAAGCCC GGTCGGAAGC CAACACCAGT GGTCTCCTGT GGGCCGGCTT 
AGCTCTGCTC TCCGTGCAGG GCGGGGCGCT GGCCTGGCTT ACTTGGTGGG TGTACTCCTG 
GGATATCATG GAGCCAGTTA CCTTCTTCCT GTCGTTTGCG AATTCCATCG TCTTTTTTGC 
ATACTTCATC ATAACCCGGC AGAACTACAC ATACTCATCC CTCCGGAGCA GGCAGTTTCT 
TCAGTTCTTC CACAAGAAAT CGCAGCGGCG GTGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280573.1
CDS	132..890
Translation	MPGALSGRRMLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPKYHQALHYGTVEPQDEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITADGSEIPASTLMDTLLMTDFKLIINKLRYDIRCHKKGQSCNRSPVGSQHQWSPVGRLSSALRAGRGAGLAYLVGVLLGYHGASYLLPVVCEFHRLFCILHHNPAELHILIPPEQAVSSVLPQEIAAAVF

Target ORF information:

RefSeq Version	NM_001293644.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293644.1

ORF Insert Sequence:

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGATGGCAG TGAAATTCCA GCTTCAACAT TGATGGACAC TTTGCTAATG 
ACAGATTTTA AGCTCATCAT TAACAAACTA CGGTATGATA TACGGTGTCA CAAGAAAGGT 
CAAAGCTGCA ATAGAAGCCC GGTCGGAAGC CAACACCAGT GGTCTCCTGT GGGCCGGCTT 
AGCTCTGCTC TCCGTGCAGG GCGGGGCGCT GGCCTGGCTT ACTTGGTGGG TGTACTCCTG 
GGATATCATG GAGCCAGTTA CCTTCTTCCT GTCGTTTGCG AATTCCATCG TCTTTTTTGC 
ATACTTCATC ATAACCCGGC AGAACTACAC ATACTCATCC CTCCGGAGCA GGCAGTTTCT 
TCAGTTCTTC CACAAGAAAT CGCAGCGGCG GTGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcub ( XM_006501900.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501963.1 Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X1, mRNA.

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CloneID	OMu32179
Clone ID Related Accession (Same CDS sequence)	XM_006501900.1 , XM_006501900.2
Accession Version	XM_006501900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGAAGAGCC AAGTGGAGAG CATATGACTG AGTTGGAAAA CACCAAGTCC 
TTGGTTCACA GACTATTCAC TATCCTGCAT TTAGAGGAGA TCCAGAAGAG ACGAGAGCGC 
CATCTGATGG CGAAGATCGA CCACCTGCAG GAACAGCTGC GGCCCTTGGA GCAGGTCAAA 
GCTGCAATAG AAGCCCGGTC GGAAGCCAAC ACCAGTGGTC TCCTGTGGGC CGGCTTAGCT 
CTGCTCTCCG TGCAGGGCGG GGCGCTGGCC TGGCTTACTT GGTGGGTGTA CTCCTGGGAT 
ATCATGGAGC CAGTTACCTT CTTCCTGTCG TTTGCGAATT CCATCGTCTT TTTTGCATAC 
TTCATCATAA CCCGGCAGAA CTACACATAC TCATCCCTCC GGAGCAGGCA GTTTCTTCAG 
TTCTTCCACA AGAAATCGCA GCGGCGGTGT TTTGATGTGG AACAATACAA CAAGCTAAAG 
GAAGACCTTG CTGAGGCTAC AGAATCCCTG GAAAGCGTGC GCCGCTCCCT CCGCTTGCGA 
ATCCAAGGAG AGGAAGCCAG CGAGAAGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501963.1
CDS	137..1069
Translation	MPGALSGRRMLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPKYHQALHYGTVEPQDEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITAEEPSGEHMTELENTKSLVHRLFTILHLEEIQKRRERHLMAKIDHLQEQLRPLEQVKAAIEARSEANTSGLLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLSFANSIVFFAYFIITRQNYTYSSLRSRQFLQFFHKKSQRRCFDVEQYNKLKEDLAEATESLESVRRSLRLRIQGEEASEKN

Target ORF information:

RefSeq Version	XM_006501900.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501900.2

ORF Insert Sequence:

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGAAGAGCC AAGTGGAGAG CATATGACTG AGTTGGAAAA CACCAAGTCC 
TTGGTTCACA GACTATTCAC TATCCTGCAT TTAGAGGAGA TCCAGAAGAG ACGAGAGCGC 
CATCTGATGG CGAAGATCGA CCACCTGCAG GAACAGCTGC GGCCCTTGGA GCAGGTCAAA 
GCTGCAATAG AAGCCCGGTC GGAAGCCAAC ACCAGTGGTC TCCTGTGGGC CGGCTTAGCT 
CTGCTCTCCG TGCAGGGCGG GGCGCTGGCC TGGCTTACTT GGTGGGTGTA CTCCTGGGAT 
ATCATGGAGC CAGTTACCTT CTTCCTGTCG TTTGCGAATT CCATCGTCTT TTTTGCATAC 
TTCATCATAA CCCGGCAGAA CTACACATAC TCATCCCTCC GGAGCAGGCA GTTTCTTCAG 
TTCTTCCACA AGAAATCGCA GCGGCGGTGT TTTGATGTGG AACAATACAA CAAGCTAAAG 
GAAGACCTTG CTGAGGCTAC AGAATCCCTG GAAAGCGTGC GCCGCTCCCT CCGCTTGCGA 
ATCCAAGGAG AGGAAGCCAG CGAGAAGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcub ( XM_006501901.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501964.1 Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X2, mRNA.

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CloneID	OMu32180
Clone ID Related Accession (Same CDS sequence)	XM_006501901.3 , XM_006501901.4
Accession Version	XM_006501901.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501901.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGGT CGGTGGCAGC TGCTGCGGAC GATCCGAGCC CGCGGCCGCG GGGATCCCAG 
GGAGCTGCCA TCCACACCCC AGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGATGGCAG TGAAATTCCA GCTTCAACAT TGATGGACAC TTTGCTAATG 
ACAGATTTTA AGCTCATCAT TAACAAACTA CGGTATGATA TACGGTGTCA CAAGAAAGAA 
GAGCCAAGTG GAGAGCATAT GACTGAGTTG GAAAACACCA AGTCCTTGGT TCACAGACTA 
TTCACTATCC TGCATTTAGA GGAGATCCAG AAGAGACGAG AGCGCCATCT GATGGCGAAG 
ATCGACCACC TGCAGGAACA GCTGCGGCCC TTGGAGCAGG TCAAAGCTGC AATAGAAGCC 
CGGTCGGAAG CCAACACCAG TGGTCTCCTG TGGGCCGGCT TAGCTCTGCT CTCCGTGCAG 
GGCGGGGCGC TGGCCTGGCT TACTTGGTGG GTGTACTCCT GGGATATCAT GGAGCCAGTT 
ACCTTCTTCC TGTCGTTTGC GAATTCCATC GTCTTTTTTG CATACTTCAT CATAACCCGG 
CAGAACTACA CATACTCATC CCTCCGGAGC AGGCAGTTTC TTCAGTTCTT CCACAAGAAA 
TCGCAGCGGC GGTGTTTTGA TGTGGAACAA TACAACAAGC TAAAGGAAGA CCTTGCTGAG 
GCTACAGAAT CCCTGGAAAG CGTGCGCCGC TCCCTCCGCT TGCGAATCCA AGGAGAGGAA 
GCCAGCGAGA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501964.1
CDS	181..1098
Translation	MSGSVAAAADDPSPRPRGSQGAAIHTPEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITADGSEIPASTLMDTLLMTDFKLIINKLRYDIRCHKKEEPSGEHMTELENTKSLVHRLFTILHLEEIQKRRERHLMAKIDHLQEQLRPLEQVKAAIEARSEANTSGLLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLSFANSIVFFAYFIITRQNYTYSSLRSRQFLQFFHKKSQRRCFDVEQYNKLKEDLAEATESLESVRRSLRLRIQGEEASEKN

Target ORF information:

RefSeq Version	XM_006501901.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501901.4

ORF Insert Sequence:

ATGTCTGGGT CGGTGGCAGC TGCTGCGGAC GATCCGAGCC CGCGGCCGCG GGGATCCCAG 
GGAGCTGCCA TCCACACCCC AGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGATGGCAG TGAAATTCCA GCTTCAACAT TGATGGACAC TTTGCTAATG 
ACAGATTTTA AGCTCATCAT TAACAAACTA CGGTATGATA TACGGTGTCA CAAGAAAGAA 
GAGCCAAGTG GAGAGCATAT GACTGAGTTG GAAAACACCA AGTCCTTGGT TCACAGACTA 
TTCACTATCC TGCATTTAGA GGAGATCCAG AAGAGACGAG AGCGCCATCT GATGGCGAAG 
ATCGACCACC TGCAGGAACA GCTGCGGCCC TTGGAGCAGG TCAAAGCTGC AATAGAAGCC 
CGGTCGGAAG CCAACACCAG TGGTCTCCTG TGGGCCGGCT TAGCTCTGCT CTCCGTGCAG 
GGCGGGGCGC TGGCCTGGCT TACTTGGTGG GTGTACTCCT GGGATATCAT GGAGCCAGTT 
ACCTTCTTCC TGTCGTTTGC GAATTCCATC GTCTTTTTTG CATACTTCAT CATAACCCGG 
CAGAACTACA CATACTCATC CCTCCGGAGC AGGCAGTTTC TTCAGTTCTT CCACAAGAAA 
TCGCAGCGGC GGTGTTTTGA TGTGGAACAA TACAACAAGC TAAAGGAAGA CCTTGCTGAG 
GCTACAGAAT CCCTGGAAAG CGTGCGCCGC TCCCTCCGCT TGCGAATCCA AGGAGAGGAA 
GCCAGCGAGA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcub ( XM_011240197.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238499.1 Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu70013
Clone ID Related Accession (Same CDS sequence)	XM_011240197.2 , XM_011240197.3
Accession Version	XM_011240197.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium uniporter regulatory subunit MCUb, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240197.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGGTCAAAG CTGCAATAGA AGCCCGGTCG GAAGCCAACA CCAGTGGTCT 
CCTGTGGGCC GGCTTAGCTC TGCTCTCCGT GCAGGGCGGG GCGCTGGCCT GGCTTACTTG 
GTGGGTGTAC TCCTGGGATA TCATGGAGCC AGTTACCTTC TTCCTGTCGT TTGCGAATTC 
CATCGTCTTT TTTGCATACT TCATCATAAC CCGGCAGAAC TACACATACT CATCCCTCCG 
GAGCAGGCAG TTTCTTCAGT TCTTCCACAA GAAATCGCAG CGGCGGTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238499.1
CDS	135..788
Translation	MPGALSGRRMLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPKYHQALHYGTVEPQDEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNEDKGIKTAAIITAGQSCNRSPVGSQHQWSPVGRLSSALRAGRGAGLAYLVGVLLGYHGASYLLPVVCEFHRLFCILHHNPAELHILIPPEQAVSSVLPQEIAAAVF

Target ORF information:

RefSeq Version	XM_011240197.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial calcium uniporter dominant negative beta subunit (Mcub), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240197.3

ORF Insert Sequence:

ATGCCAGGAG CTCTGTCCGG CAGGAGGATG CTCCCGAGCG GGCTATGTCT GGGTCGGTGG 
CAGCTGCTGC GGACGATCCG AGCCCGCGGC CGCGGGGATC CCAGGGAGCT GCCATCCACA 
CCCCAGGTTT TATGTATGAA ACTCTATGGA AATCCAAAAT ACCACCAAGC ACTCCATTAT 
GGGACAGTGG AGCCACAGGA TGAAATAACA GTTACTTATA AACACGGCCT TCCCTTGGTA 
ACCCTCACTC TGCCATCCAG AAAAGAGCGC TGCCAGTTTG TAGTCAAGCC GATGTTGTCG 
ACAGTAGGCT CATTCCTGCA GGACTTACAG AATGAGGATA AAGGCATCAA AACTGCAGCC 
ATCATCACGG CAGGTCAAAG CTGCAATAGA AGCCCGGTCG GAAGCCAACA CCAGTGGTCT 
CCTGTGGGCC GGCTTAGCTC TGCTCTCCGT GCAGGGCGGG GCGCTGGCCT GGCTTACTTG 
GTGGGTGTAC TCCTGGGATA TCATGGAGCC AGTTACCTTC TTCCTGTCGT TTGCGAATTC 
CATCGTCTTT TTTGCATACT TCATCATAAC CCGGCAGAAC TACACATACT CATCCCTCCG 
GAGCAGGCAG TTTCTTCAGT TCTTCCACAA GAAATCGCAG CGGCGGTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Expression of mRNA Encoding Mcu and Other Mitochondrial Calcium Regulatory Genes Depends on Cell Type, Neuronal Subtype, and Ca2+ Signaling.
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Mcub cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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