Uqcrfs1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Uqcrfs1
Entrez Gene ID	66694
Full Name	ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
Synonyms	4430402G14Rik,AI875505
General protein information	Preferred Names ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 Names cytochrome b-c1 complex subunit Rieske, mitochondrial RISP Rieske protein UQCRFS1 complex III subunit 5 cytochrome b-c1 complex subunit 5 rieske iron-sulfur protein ubiquinol-cytochrome c reductase iron-sulfur subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A3.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_025710.2	NP_079986.1	cytochrome b-c1 complex subunit Rieske, mitochondrial

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100334857	XP_003199036.1
Drosophila melanogaster (fruit fly)	RFeSP	NP_722715.1
Kluyveromyces lactis	KLLA0E22573g	XP_454973.1
Schizosaccharomyces pombe (fission yeast)	rip1	NP_595941.1
Neurospora crassa	NCU06606	XP_962348.1
Oryza sativa (rice)	Os04g0398500	NP_001052672.1
Bos taurus (cattle)	UQCRFS1	NP_777238.1
Mus musculus (house mouse)	Uqcrfs1	NP_079986.1
	UQCRFS1	XP_001101289.1
Canis lupus familiaris (dog)	UQCRFS1	XP_533711.1
Rattus norvegicus (Norway rat)	Uqcrfs1	NP_001008888.1
Gallus gallus (chicken)	UQCRFS1	NP_001005843.1
Danio rerio (zebrafish)	uqcrfs1	NP_001096664.1
Arabidopsis thaliana (thale cress)	AT5G13440	NP_196848.1
Arabidopsis thaliana (thale cress)	AT5G13430	NP_568288.1
Xenopus tropicalis (tropical clawed frog)	uqcrfs1	NP_988911.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008955	XP_319708.4
Caenorhabditis elegans (roundworm)	isp-1	NP_501361.1
Eremothecium gossypii	AGOS_AEL067W	NP_984794.1
Magnaporthe oryzae (rice blast fungus)	MGG_16866	XP_003712616.1
Homo sapiens (human)	UQCRFS1	NP_005994.2
Pan troglodytes (chimpanzee)	UQCRFS1	XP_001148728.1
Saccharomyces cerevisiae (baker's yeast)	RIP1	NP_010890.3
Oryza sativa (rice)	Os02g0520800	NP_001046975.1

Gene Ontology
Bibliography

Related articles in PubMed

Editing activity for eliminating mischarged tRNAs is essential in mammalian mitochondria.
Hilander T, Zhou XL, Konovalova S, Zhang FP, Euro L, Chilov D, Poutanen M, Chihade J, Wang ED, Tyynismaa H
Nucleic acids research(2017 Dec)

TTC19 Plays a Husbandry Role on UQCRFS1 Turnover in the Biogenesis of Mitochondrial Respiratory Complex III.
Bottani E, Cerutti R, Harbour ME, Ravaglia S, Dogan SA, Giordano C, Fearnley IM, D'Amati G, Viscomi C, Fernandez-Vizarra E, Zeviani M
Molecular cell67(1)96-105.e4(2017 Jul)

The mitochondrial respiratory chain is essential for haematopoietic stem cell function.
Ans? E, Weinberg SE, Diebold LP, Thompson BJ, Malinge S, Schumacker PT, Liu X, Zhang Y, Shao Z, Steadman M, Marsh KM, Xu J, Crispino JD, Chandel NS
Nature cell biology19(6)614-625(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UQCRFS1 produces N-terminal polypeptides, which remain bound to holocomplex III. We show that, in normal conditions, these UQCRFS1 fragments are rapidly removed, but when TTC19 is absent they accumulate within complex III, causing its structural and functional impairment.
Title: TTC19 Plays a Husbandry Role on UQCRFS1 Turnover in the Biogenesis of Mitochondrial Respiratory Complex III.

Rieske iron-sulfur protein is required for superoxide generation at Complex III.
Title: Superoxide generated at mitochondrial complex III triggers acute responses to hypoxia in the pulmonary circulation.

Mitochondrial respiratory complexes of the electron transport chain (CI and CIV) and supercomplexes were decreased in the RISP-deficient cells.
Title: Cells lacking Rieske iron-sulfur protein have a reactive oxygen species-associated decrease in respiratory complexes I and IV.

A 3' UTR modification of the mitochondrial rieske iron sulfur protein in mice produces a specific skin pigmentation phenotype.
Title: A 3' UTR modification of the mitochondrial rieske iron sulfur protein in mice produces a specific skin pigmentation phenotype.

The following Uqcrfs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Uqcrfs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13183	NM_025710.2 Latest version!	Mus musculus ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Uqcrfs1 ( NM_025710.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079986.1 Mus musculus ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1), mRNA.

Price & Availability↑

CloneID	OMu13183
Clone ID Related Accession (Same CDS sequence)	NM_025710.2
Accession Version	NM_025710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	cytochrome b-c1 complex subunit Rieske, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK014470.1. On Aug 5, 2008 this sequence version replaced NM_025710.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK014470.1, AK152391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTTGTCGG TCGCCGCCCG CTCGGGCCCG TTCGCGCCCG TCCTATCGGC CACTTCCCGC 
GGGGTGGCGG GCGCGCTGCG TCCTCTGCTG CAAGGCGCGG TGCCCGCCGC CTCGGAGCCA 
CCTGTTCTGG ATGTGAAGCG ACCCTTCCTG TGCCGCGAGT CCCTGAGTGG CCAGGCCGCA 
GCCCGGCCTT TGGTGGCCAC GGTGGGCCTG AACGTTCCTG CTTCTGTCCG TTTTTCCCAT 
ACAGATGTCA AGGTGCCCGA CTTCTCTGAC TATCGTCGTG CTGAAGTTCT TGATAGCACA 
AAATCTTCTA AAGAAAGCAG TGAGGCTAGA AAAGGCTTCT CTTACCTGGT AACTGCAACT 
ACTACTGTGG GTGTTGCTTA TGCGGCCAAA AATGTGGTCT CCCAGTTTGT TTCCAGCATG 
AGTGCTTCTG CTGACGTACT GGCCATGTCG AAGATCGAGA TCAAGTTGTC TGATATCCCT 
GAAGGGAAGA ACATGGCTTT TAAATGGAGA GGCAAACCTC TGTTTGTGCG CCATAGAACC 
AAGAAGGAGA TTGACCAGGA AGCTGCAGTC GAAGTGTCCC AGTTAAGGGA CCCACAGCAT 
GATTTAGATC GAGTAAAGAA ACCTGAATGG GTTATTCTGA TAGGTGTCTG TACTCATCTT 
GGTTGTGTAC CCATTGCAAA CGCAGGAGAT TTTGGTGGCT ATTATTGCCC CTGCCATGGG 
TCACACTATG ACGCCTCTGG CAGGATCAGG AAGGGCCCTG CACCTCTCAA CCTGGAAGTG 
CCTGCCTACG AGTTCACCAG TGATGATGTA GTTGTTGTGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079986.1
CDS	70..894
Translation	MLSVAARSGPFAPVLSATSRGVAGALRPLLQGAVPAASEPPVLDVKRPFLCRESLSGQAAARPLVATVGLNVPASVRFSHTDVKVPDFSDYRRAEVLDSTKSSKESSEARKGFSYLVTATTTVGVAYAAKNVVSQFVSSMSASADVLAMSKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTKKEIDQEAAVEVSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRKGPAPLNLEVPAYEFTSDDVVVVG

Target ORF information:

RefSeq Version	NM_025710.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025710.2

ORF Insert Sequence:

ATGTTGTCGG TCGCCGCCCG CTCGGGCCCG TTCGCGCCCG TCCTATCGGC CACTTCCCGC 
GGGGTGGCGG GCGCGCTGCG TCCTCTGCTG CAAGGCGCGG TGCCCGCCGC CTCGGAGCCA 
CCTGTTCTGG ATGTGAAGCG ACCCTTCCTG TGCCGCGAGT CCCTGAGTGG CCAGGCCGCA 
GCCCGGCCTT TGGTGGCCAC GGTGGGCCTG AACGTTCCTG CTTCTGTCCG TTTTTCCCAT 
ACAGATGTCA AGGTGCCCGA CTTCTCTGAC TATCGTCGTG CTGAAGTTCT TGATAGCACA 
AAATCTTCTA AAGAAAGCAG TGAGGCTAGA AAAGGCTTCT CTTACCTGGT AACTGCAACT 
ACTACTGTGG GTGTTGCTTA TGCGGCCAAA AATGTGGTCT CCCAGTTTGT TTCCAGCATG 
AGTGCTTCTG CTGACGTACT GGCCATGTCG AAGATCGAGA TCAAGTTGTC TGATATCCCT 
GAAGGGAAGA ACATGGCTTT TAAATGGAGA GGCAAACCTC TGTTTGTGCG CCATAGAACC 
AAGAAGGAGA TTGACCAGGA AGCTGCAGTC GAAGTGTCCC AGTTAAGGGA CCCACAGCAT 
GATTTAGATC GAGTAAAGAA ACCTGAATGG GTTATTCTGA TAGGTGTCTG TACTCATCTT 
GGTTGTGTAC CCATTGCAAA CGCAGGAGAT TTTGGTGGCT ATTATTGCCC CTGCCATGGG 
TCACACTATG ACGCCTCTGG CAGGATCAGG AAGGGCCCTG CACCTCTCAA CCTGGAAGTG 
CCTGCCTACG AGTTCACCAG TGATGATGTA GTTGTTGTGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Editing activity for eliminating mischarged tRNAs is essential in mammalian mitochondria.
Nucleic acids research(2017 Dec)
Hilander T,Zhou XL,Konovalova S,Zhang FP,Euro L,Chilov D,Poutanen M,Chihade J,Wang ED,Tyynismaa H

TTC19 Plays a Husbandry Role on UQCRFS1 Turnover in the Biogenesis of Mitochondrial Respiratory Complex III.
Molecular cell67(1)96-105.e4(2017 Jul)
Bottani E,Cerutti R,Harbour ME,Ravaglia S,Dogan SA,Giordano C,Fearnley IM,D'Amati G,Viscomi C,Fernandez-Vizarra E,Zeviani M

The mitochondrial respiratory chain is essential for haematopoietic stem cell function.
Nature cell biology19(6)614-625(2017 Jun)
Ans? E,Weinberg SE,Diebold LP,Thompson BJ,Malinge S,Schumacker PT,Liu X,Zhang Y,Shao Z,Steadman M,Marsh KM,Xu J,Crispino JD,Chandel NS

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Uqcrfs1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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