Gemin2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gemin2
Entrez Gene ID	66603
Full Name	gem nuclear organelle associated protein 2
Synonyms	Sip1,gemin-2
General protein information	Preferred Names gem nuclear organelle associated protein 2 Names gem-associated protein 2 SMN-interacting protein 1 component of gems 2 survival of motor neuron protein-interacting protein 1 survivor of motor neuron protein interacting protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C1
Summary	This gene encodes one of the proteins found in the survival of motor neuron (SMN) complex, which consists of the SMN protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal small nuclear ribonucleoproteins (snRNP) and for pre-mRNA splicing. This protein interacts directly with the SMN protein and it is required for formation of the SMN complex. Disruption of this gene in mouse resulted in impaired snRNP assembly, and motor neuron degeneration. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_025656.5	NP_079932.2	gem-associated protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIP1	XP_509917.1
Danio rerio (zebrafish)	gemin2	NP_001017608.1
Homo sapiens (human)	GEMIN2	NP_003607.1
	SIP1	XP_001092707.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005413	XP_001688660.1
Canis lupus familiaris (dog)	GEMIN2	XP_005623408.1
Bos taurus (cattle)	GEMIN2	XP_003587627.1
Rattus norvegicus (Norway rat)	Gemin2	NP_445841.1
Gallus gallus (chicken)	GEMIN2	NP_001034391.1
Mus musculus (house mouse)	Gemin2	NP_079932.2
Drosophila melanogaster (fruit fly)	Gem2	NP_649092.1
Xenopus tropicalis (tropical clawed frog)	gemin2	NP_001096228.1

Gene Ontology
Bibliography

Related articles in PubMed

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Tang F, Barbacioru C, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA
PloS one6(6)e21208(2011)

SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro.
Todd AG, Morse R, Shaw DJ, McGinley S, Stebbings H, Young PJ
Journal of molecular biology401(5)681-9(2010 Sep)

The activity of the spinal muscular atrophy protein is regulated during development and cellular differentiation.
Gabanella F, Carissimi C, Usiello A, Pellizzoni L
Human molecular genetics14(23)3629-42(2005 Dec)

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
McKee AE, Minet E, Stern C, Riahi S, Stiles CD, Silver PA
BMC developmental biology514(2005 Jul)

Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell death.
Jablonka S, Holtmann B, Meister G, Bandilla M, Rossoll W, Fischer U, Sendtner M
Proceedings of the National Academy of Sciences of the United States of America99(15)10126-31(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

utilised a targeted screen to identify mRNA associated with SMN, Gemin2 and Gemin3 in the cytoplasm of a human neuroblastoma cell line, SHSY5Y
Title: SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro.

Gene targeting in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell death (gemin2; SMN interacting protein 1)
Title: Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell death.

The following Gemin2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gemin2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00828	NM_025656.5 Latest version!	Mus musculus gem nuclear organelle associated protein 2 (Gemin2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gemin2 ( NM_025656.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079932.2 Mus musculus gem nuclear organelle associated protein 2 (Gemin2), mRNA.

Price & Availability↑

CloneID	OMu00828
Clone ID Related Accession (Same CDS sequence)	NM_025656.5
Accession Version	NM_025656.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	gem-associated protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB623237.1, AK005928.1 and AC153654.4. On Sep 19, 2015 this sequence version replaced NM_025656.4. ##Evidence-Data-START## Transcript exon combination :: AK005928.1, AK013414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTGGG TTCCAGCCGA GTCTGCGGTG GAAGAGTTGA TGCCACGGTT GTTACCTGTG 
GAGCCCTGTG ACTTGACGGA AGGTTTCGAT CCCTCGGTCC CCCCCAGGAC GCCTCAGGAA 
TATCTGAGGA GAGTGCAGAT CGAAGCAGCC CAATGTCCAG ATGTTGTGGT AGCCCAGATT 
GACCCAAAGA AGTTGAAAAG GAAGCAAAGT GTGAACATTT CTCTTTCCGG ATGCCAGCCT 
GCGCCTGAAG GTTACTCTCC AACACTTCAG TGGCAACAAC AACAAGTGGC ACATTTTTCA 
ACTGTTCGAC AGAGTGTACA CAAGCATAGA AATCACTGGA AATCACAACA GTTGGACAGT 
AATGTGGCAA TGCCAAAATC TGAAGATGAA GAAGGCTGGA AAAAATTTTG TCTGGGTGAA 
AGGTTATGTG CTGAAGGGGC CACTGGACCG TCTACAGAGG AAAGCCCTGG GATCGATTAT 
GTACAAGTTG GTTTTCCTCC TTTGCTTAGT ATTGTAAGCA GAATGAATCA GACAACCATT 
ACTAGTGTCT TGGAATATCT AAGTAATTGG TTTGGAGAAA GAGACTTTAC TCCAGAATTG 
GGAAGATGGT TTTATGCTTT GTTGGCTTGT CTTGAAAAGC CTTTATTACC TGAGGCTCAT 
TCACTAATCC GACAGCTTGC AAGAAGATGT TCTGAAGTGA GGCTGCTGGT GGGCAGTAAA 
GATGACGAAA GAGTCCCAGC TTTGAATCTG CTGATCTGCT TAGTTAGCAG GTATTTTGAT 
CAACGCGATT TAGCTGATGA GCCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079932.2
CDS	87..896
Translation	MAWVPAESAVEELMPRLLPVEPCDLTEGFDPSVPPRTPQEYLRRVQIEAAQCPDVVVAQIDPKKLKRKQSVNISLSGCQPAPEGYSPTLQWQQQQVAHFSTVRQSVHKHRNHWKSQQLDSNVAMPKSEDEEGWKKFCLGERLCAEGATGPSTEESPGIDYVQVGFPPLLSIVSRMNQTTITSVLEYLSNWFGERDFTPELGRWFYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVGSKDDERVPALNLLICLVSRYFDQRDLADEPS

Target ORF information:

RefSeq Version	NM_025656.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus gem nuclear organelle associated protein 2 (Gemin2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025656.5

ORF Insert Sequence:

ATGGCGTGGG TTCCAGCCGA GTCTGCGGTG GAAGAGTTGA TGCCACGGTT GTTACCTGTG 
GAGCCCTGTG ACTTGACGGA AGGTTTCGAT CCCTCGGTCC CCCCCAGGAC GCCTCAGGAA 
TATCTGAGGA GAGTGCAGAT CGAAGCAGCC CAATGTCCAG ATGTTGTGGT AGCCCAGATT 
GACCCAAAGA AGTTGAAAAG GAAGCAAAGT GTGAACATTT CTCTTTCCGG ATGCCAGCCT 
GCGCCTGAAG GTTACTCTCC AACACTTCAG TGGCAACAAC AACAAGTGGC ACATTTTTCA 
ACTGTTCGAC AGAGTGTACA CAAGCATAGA AATCACTGGA AATCACAACA GTTGGACAGT 
AATGTGGCAA TGCCAAAATC TGAAGATGAA GAAGGCTGGA AAAAATTTTG TCTGGGTGAA 
AGGTTATGTG CTGAAGGGGC CACTGGACCG TCTACAGAGG AAAGCCCTGG GATCGATTAT 
GTACAAGTTG GTTTTCCTCC TTTGCTTAGT ATTGTAAGCA GAATGAATCA GACAACCATT 
ACTAGTGTCT TGGAATATCT AAGTAATTGG TTTGGAGAAA GAGACTTTAC TCCAGAATTG 
GGAAGATGGT TTTATGCTTT GTTGGCTTGT CTTGAAAAGC CTTTATTACC TGAGGCTCAT 
TCACTAATCC GACAGCTTGC AAGAAGATGT TCTGAAGTGA GGCTGCTGGT GGGCAGTAAA 
GATGACGAAA GAGTCCCAGC TTTGAATCTG CTGATCTGCT TAGTTAGCAG GTATTTTGAT 
CAACGCGATT TAGCTGATGA GCCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
PloS one6(6)e21208(2011)
Tang F,Barbacioru C,Nordman E,Bao S,Lee C,Wang X,Tuch BB,Heard E,Lao K,Surani MA

SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro.
Journal of molecular biology401(5)681-9(2010 Sep)
Todd AG,Morse R,Shaw DJ,McGinley S,Stebbings H,Young PJ

The activity of the spinal muscular atrophy protein is regulated during development and cellular differentiation.
Human molecular genetics14(23)3629-42(2005 Dec)
Gabanella F,Carissimi C,Usiello A,Pellizzoni L

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
BMC developmental biology514(2005 Jul)
McKee AE,Minet E,Stern C,Riahi S,Stiles CD,Silver PA

Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell death.
Proceedings of the National Academy of Sciences of the United States of America99(15)10126-31(2002 Jul)
Jablonka S,Holtmann B,Meister G,Bandilla M,Rossoll W,Fischer U,Sendtner M

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Gemin2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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