Gtf3a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gtf3a
Entrez Gene ID	66596
Full Name	general transcription factor III A
Synonyms	2010015D03Rik,2610111I01Rik,5330403M05Rik
General protein information	Preferred Names general transcription factor III A Names transcription factor IIIA TFIIIA factor A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G3
Summary	The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_025652.3	NP_079928.2	transcription factor IIIA

Pathways from BioSystems

Homologies

Homo sapiens (human)	GTF3A	NP_002088.2
Mus musculus (house mouse)	Gtf3a	NP_079928.2
Rattus norvegicus (Norway rat)	Gtf3a	NP_001107042.1
Gallus gallus (chicken)	GTF3A	NP_001107043.1
Drosophila melanogaster (fruit fly)	CG9609	NP_573161.1
Magnaporthe oryzae (rice blast fungus)	MGG_05322	XP_003712886.1
Pan troglodytes (chimpanzee)	GTF3A	XP_522641.3
	GTF3A	XP_001095556.1
Canis lupus familiaris (dog)	GTF3A	XP_534523.3
Bos taurus (cattle)	GTF3A	NP_001107044.1
Danio rerio (zebrafish)	gtf3aa	NP_001003866.1
Schizosaccharomyces pombe (fission yeast)	sfc2	NP_594670.1
Xenopus tropicalis (tropical clawed frog)	gtf3a	XP_002941582.2

Gene Ontology
Bibliography

Related articles in PubMed

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

cDNA cloning, DNA binding, and evolution of mammalian transcription factor IIIA.
Hanas JS, Hocker JR, Cheng YG, Lerner MR, Brackett DJ, Lightfoot SA, Hanas RJ, Madhusudhan KT, Moreland RJ
Gene282(1-2)43-52(2002 Jan)

Purification and characterization of human transcription factor IIIA.
Moorefield B, Roeder RG
The Journal of biological chemistry269(33)20857-65(1994 Aug)

Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes.
Kozak M
Proceedings of the National Academy of Sciences of the United States of America87(21)8301-5(1990 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gtf3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gtf3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13020	NM_025652.3 Latest version!	Mus musculus general transcription factor III A (Gtf3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gtf3a ( NM_025652.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079928.2 Mus musculus general transcription factor III A (Gtf3a), mRNA.

Price & Availability↑

CloneID	OMu13020
Clone ID Related Accession (Same CDS sequence)	NM_025652.3
Accession Version	NM_025652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	transcription factor IIIA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE656279.1, AF391799.1, AC124828.14 and BI202484.1. On Feb 8, 2008 this sequence version replaced NM_025652.2. ##Evidence-Data-START## Transcript exon combination :: AF391799.1, AK008247.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGAGCCGC GGGTGTCAGT CGCGGAAGCG GTGTCGTCGC TGACCATCGC GGATGCGTTC 
GTCGGGGCCT GTGAGGGCCC CGCGCCGCCG CGCCCCGCGC TGCCCAGCAG GTTCATCTGC 
TCCTTTCCCG ACTGCAGCGC CAGTTACAAC AAAGCCTGGA AGCTAGACGC GCACCTATGC 
AAACACACGG GGGAGAGGCC ATTTGTTTGC GACTATGAGG GCTGTGGCAA GGCCTTCATC 
AGAGACTACC ATCTGAGCCG GCATGTCCTG ATTCACACCG GGGAAAAGCC GTTTGTTTGT 
GCAGATGATG GCTGTAATCA GAAATTCAAC ACAAAATCAA ACTTGAAGAA ACACATTGAA 
CGCAAACATG GAAACCCACA AAAACAGTAT GTGTGCAGTT ATGAGGGTTG CAAGAAGGCC 
TTTAAGAAGC ACCAGCAGCT GAGAACCCAT CAGTGCCAGC ACACCAGCGA GCCGCTCTTC 
AGGTGTACCC ACGAGGGATG CGGGAAGCAC TTTGCCTCGC CCAGCAGGCT GAAACGGCAT 
GGGAAAGTTC ACGAGGGCTA CCTGTGTCAA AAGGGATGTT CTTTCATGGG AAAAACGTGG 
ACAGAGCTCC TGAAACACAT GAGAGAAGCC CATAAAGAGG ACATAACCTG CAATGTATGT 
CAGAGGATGT TCAAGCGCAG AGATTACCTT AAGCAGCACA TGAAGACTCA CGCCCCGGAA 
AGGGATGTGT ACCGCTGTCC GCGGCAAGGC TGCGGAAGAA CCTACACAAC CGTGTTCAAC 
CTGCAGAGCC ACATTCTCTC CTTCCACGAG GAAAAGCGCC CATTTGTGTG TGAGCACGCT 
GGCTGTGGCA AGACATTCGC AATGAAACAG AGTCTCATGA GGCACAGTGT CGTGCACGAT 
CCCGACAAGA AGAGGATGAA GCTCAAAGTA AGAGCCCCTC GGGAGAGACG CAGCTTGGCC 
TCTCGCCTCA GTGGGTACTT CCCTCCTAAG AGGAAACAAG AGCCCGACTA CTCCTTGCCT 
AACGCCAGCG CAGAGTCCAG CAGCAGCCCA GAGGCCCAGC TGCCCCCGCC AGCCGCCTTA 
CTCACTGTCT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079928.2
CDS	125..1219
Translation	LEPRVSVAEAVSSLTIADAFVGACEGPAPPRPALPSRFICSFPDCSASYNKAWKLDAHLCKHTGERPFVCDYEGCGKAFIRDYHLSRHVLIHTGEKPFVCADDGCNQKFNTKSNLKKHIERKHGNPQKQYVCSYEGCKKAFKKHQQLRTHQCQHTSEPLFRCTHEGCGKHFASPSRLKRHGKVHEGYLCQKGCSFMGKTWTELLKHMREAHKEDITCNVCQRMFKRRDYLKQHMKTHAPERDVYRCPRQGCGRTYTTVFNLQSHILSFHEEKRPFVCEHAGCGKTFAMKQSLMRHSVVHDPDKKRMKLKVRAPRERRSLASRLSGYFPPKRKQEPDYSLPNASAESSSSPEAQLPPPAALLTVC

Target ORF information:

RefSeq Version	NM_025652.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor III A (Gtf3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025652.3

ORF Insert Sequence:

CTGGAGCCGC GGGTGTCAGT CGCGGAAGCG GTGTCGTCGC TGACCATCGC GGATGCGTTC 
GTCGGGGCCT GTGAGGGCCC CGCGCCGCCG CGCCCCGCGC TGCCCAGCAG GTTCATCTGC 
TCCTTTCCCG ACTGCAGCGC CAGTTACAAC AAAGCCTGGA AGCTAGACGC GCACCTATGC 
AAACACACGG GGGAGAGGCC ATTTGTTTGC GACTATGAGG GCTGTGGCAA GGCCTTCATC 
AGAGACTACC ATCTGAGCCG GCATGTCCTG ATTCACACCG GGGAAAAGCC GTTTGTTTGT 
GCAGATGATG GCTGTAATCA GAAATTCAAC ACAAAATCAA ACTTGAAGAA ACACATTGAA 
CGCAAACATG GAAACCCACA AAAACAGTAT GTGTGCAGTT ATGAGGGTTG CAAGAAGGCC 
TTTAAGAAGC ACCAGCAGCT GAGAACCCAT CAGTGCCAGC ACACCAGCGA GCCGCTCTTC 
AGGTGTACCC ACGAGGGATG CGGGAAGCAC TTTGCCTCGC CCAGCAGGCT GAAACGGCAT 
GGGAAAGTTC ACGAGGGCTA CCTGTGTCAA AAGGGATGTT CTTTCATGGG AAAAACGTGG 
ACAGAGCTCC TGAAACACAT GAGAGAAGCC CATAAAGAGG ACATAACCTG CAATGTATGT 
CAGAGGATGT TCAAGCGCAG AGATTACCTT AAGCAGCACA TGAAGACTCA CGCCCCGGAA 
AGGGATGTGT ACCGCTGTCC GCGGCAAGGC TGCGGAAGAA CCTACACAAC CGTGTTCAAC 
CTGCAGAGCC ACATTCTCTC CTTCCACGAG GAAAAGCGCC CATTTGTGTG TGAGCACGCT 
GGCTGTGGCA AGACATTCGC AATGAAACAG AGTCTCATGA GGCACAGTGT CGTGCACGAT 
CCCGACAAGA AGAGGATGAA GCTCAAAGTA AGAGCCCCTC GGGAGAGACG CAGCTTGGCC 
TCTCGCCTCA GTGGGTACTT CCCTCCTAAG AGGAAACAAG AGCCCGACTA CTCCTTGCCT 
AACGCCAGCG CAGAGTCCAG CAGCAGCCCA GAGGCCCAGC TGCCCCCGCC AGCCGCCTTA 
CTCACTGTCT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

cDNA cloning, DNA binding, and evolution of mammalian transcription factor IIIA.
Gene282(1-2)43-52(2002 Jan)
Hanas JS,Hocker JR,Cheng YG,Lerner MR,Brackett DJ,Lightfoot SA,Hanas RJ,Madhusudhan KT,Moreland RJ

Purification and characterization of human transcription factor IIIA.
The Journal of biological chemistry269(33)20857-65(1994 Aug)
Moorefield B,Roeder RG

Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes.
Proceedings of the National Academy of Sciences of the United States of America87(21)8301-5(1990 Nov)
Kozak M

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Gtf3a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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