Diablo cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Diablo
Entrez Gene ID	66593
Full Name	diablo, IAP-binding mitochondrial protein
Synonyms	0610041G12Rik,1700006L01Rik,AU040403,Smac
General protein information	Preferred Names diablo, IAP-binding mitochondrial protein Names diablo homolog, mitochondrial direct IAP binding protein with low PI direct IAP-binding protein with low pI second mitochondria-derived activator of caspase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_023232.3	NP_075721.3	diablo homolog, mitochondrial precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	diablo	NP_001016281.1
Pan troglodytes (chimpanzee)	DIABLO	XP_001150587.2
Canis lupus familiaris (dog)	DIABLO	NP_001073235.1
Bos taurus (cattle)	DIABLO	NP_001039347.1
Mus musculus (house mouse)	Diablo	NP_075721.3
Gallus gallus (chicken)	DIABLO	XP_415152.3
Homo sapiens (human)	DIABLO	NP_063940.1

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of apoptotic response by LAR family phosphatases-cIAP1 signaling during urinary tract morphogenesis.
Stewart K, Tang YC, Shafer MER, Graham-Paquin AL, Bouchard M
Proceedings of the National Academy of Sciences of the United States of America114(43)E9016-E9025(2017 Oct)

BID-dependent release of mitochondrial SMAC dampens XIAP-mediated immunity against Shigella.
Andree M, Seeger JM, Sch?ll S, Coutelle O, Wagner-Stippich D, Wiegmann K, Wunderlich CM, Brinkmann K, Broxtermann P, Witt A, Fritsch M, Martinelli P, Bielig H, Lamkemeyer T, Rugarli EI, Kaufmann T, Sterner-Kock A, Wunderlich FT, Villunger A, Martins LM, Kr?nke M, Kufer TA, Uterm?hlen O, Kashkar H
The EMBO journal33(19)2171-87(2014 Oct)

Smac-mimetic-induced epithelial cell death reduces the growth of renal cysts.
Fan LX, Zhou X, Sweeney WE Jr, Wallace DP, Avner ED, Grantham JJ, Li X
Journal of the American Society of Nephrology : JASN24(12)2010-22(2013 Dec)

Identification of a novel anti-apoptotic E3 ubiquitin ligase that ubiquitinates antagonists of inhibitor of apoptosis proteins SMAC, HtrA2, and ARTS.
Kim JB, Kim SY, Kim BM, Lee H, Kim I, Yun J, Jo Y, Oh T, Jo Y, Chae HD, Shin DY
The Journal of biological chemistry288(17)12014-21(2013 Apr)

Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis.
Yoon K, Jang HD, Lee SY
Biochemical and biophysical research communications319(2)649-54(2004 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We report that an Smac-mimetic selectively induces TNF-alpha-dependent cystic renal epithelial cell death, leading to the removal of cystic epithelial cells from renal tissues and delaying cyst formation.
Title: Smac-mimetic-induced epithelial cell death reduces the growth of renal cysts.

Results demonstrate an essential and apoptosis-independent function of SMAC in tumor suppression and provide new insights into the biology and targeting of colon cancer.
Title: An apoptosis-independent role of SMAC in tumor suppression.

Identification of a novel anti-apoptotic E3 ubiquitin ligase that ubiquitinates antagonists of inhibitor of apoptosis proteins SMAC, HtrA2, and ARTS.
Title: Identification of a novel anti-apoptotic E3 ubiquitin ligase that ubiquitinates antagonists of inhibitor of apoptosis proteins SMAC, HtrA2, and ARTS.

Loss of Smac/DIABLO alters both caspase-dependent and caspase-independent intrinsic programmed cell death.
Title: Caspase-3 deficiency reveals a physiologic role for Smac/DIABLO in regulating programmed cell death.

Membrane-associated XIAP induces mitochondrial outer membrane permeabilization leading to cytochrome c and Smac release, which is dependent on Bax and Bak.
Title: Role for X-linked Inhibitor of apoptosis protein upstream of mitochondrial permeabilization.

The following Diablo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Diablo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22337	NM_023232.3 Latest version!	Mus musculus diablo, IAP-binding mitochondrial protein (Diablo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Diablo ( NM_023232.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075721.3 Mus musculus diablo, IAP-binding mitochondrial protein (Diablo), mRNA.

Price & Availability↑

CloneID	OMu22337
Clone ID Related Accession (Same CDS sequence)	NM_023232.3
Accession Version	NM_023232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	diablo homolog, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY080746.1, AK012760.1 and AK133194.1. On Jan 24, 2006 this sequence version replaced NM_023232.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054109.1, AK002887.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGGCTC TGAGAAGTTG GGTGACTCGG AGCGTCTGCT CTCTGTTCAG GTACAGACAG 
CGTTTTCCTG TCTTGGCTAA CTCTAAGAAA CGCTGTTTCT CAGAATTGAT AAAACCATGG 
CACAAAACTG TGTTGACTGG ATTTGGCATG ACACTGTGTG CGGTTCCTAT TGCTCAGAAA 
TCGGAGCCTC AGTCTCTCAG TAACGAAGCA TTGATGAGGA GGGCTGTGTC TTTGGTAACA 
GATAGCACCT CTACCTTTCT GTCTCAAACC ACCTACGCGC TGATTGAAGC AATCACCGAG 
TATACTAAGG CTGTTTACAC GTTAGTGTCT CTGTACCGAC AATATACAAG TTTACTTGGG 
AAGATGAATT CCCAGGAGGA AGATGAGGTG TGGCAGGTAA TTATAGGAGC CAGAGTTGAG 
ATGACTTCAA AACAGCAGGA ATATTTGAAG TTGGAGACCA CTTGGATGAC AGCGGTTGGC 
CTTTCAGAGA TGGCTGCTGA GGCTGCCTAT CAAACTGGAG CAGATCAGGC CTCCATAACT 
GCCAGGAATC ACATCCAGTT GGTGAAGTCA CAGGTACAGG AGGTGCGCCA GCTCTCCCAG 
AAGGCAGAAA CCAAGTTGGC TGAAGCACAG ACAAAAGAGC TGCACCAGAA AGCACAGGAA 
GTGAGTGATG AGGGGGCTGA CCAGGAAGAG GAGGCCTACC TGCGTGAAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075721.3
CDS	45..758
Translation	MAALRSWVTRSVCSLFRYRQRFPVLANSKKRCFSELIKPWHKTVLTGFGMTLCAVPIAQKSEPQSLSNEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLVSLYRQYTSLLGKMNSQEEDEVWQVIIGARVEMTSKQQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKSQVQEVRQLSQKAETKLAEAQTKELHQKAQEVSDEGADQEEEAYLRED

Target ORF information:

RefSeq Version	NM_023232.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus diablo, IAP-binding mitochondrial protein (Diablo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023232.3

ORF Insert Sequence:

ATGGCGGCTC TGAGAAGTTG GGTGACTCGG AGCGTCTGCT CTCTGTTCAG GTACAGACAG 
CGTTTTCCTG TCTTGGCTAA CTCTAAGAAA CGCTGTTTCT CAGAATTGAT AAAACCATGG 
CACAAAACTG TGTTGACTGG ATTTGGCATG ACACTGTGTG CGGTTCCTAT TGCTCAGAAA 
TCGGAGCCTC AGTCTCTCAG TAACGAAGCA TTGATGAGGA GGGCTGTGTC TTTGGTAACA 
GATAGCACCT CTACCTTTCT GTCTCAAACC ACCTACGCGC TGATTGAAGC AATCACCGAG 
TATACTAAGG CTGTTTACAC GTTAGTGTCT CTGTACCGAC AATATACAAG TTTACTTGGG 
AAGATGAATT CCCAGGAGGA AGATGAGGTG TGGCAGGTAA TTATAGGAGC CAGAGTTGAG 
ATGACTTCAA AACAGCAGGA ATATTTGAAG TTGGAGACCA CTTGGATGAC AGCGGTTGGC 
CTTTCAGAGA TGGCTGCTGA GGCTGCCTAT CAAACTGGAG CAGATCAGGC CTCCATAACT 
GCCAGGAATC ACATCCAGTT GGTGAAGTCA CAGGTACAGG AGGTGCGCCA GCTCTCCCAG 
AAGGCAGAAA CCAAGTTGGC TGAAGCACAG ACAAAAGAGC TGCACCAGAA AGCACAGGAA 
GTGAGTGATG AGGGGGCTGA CCAGGAAGAG GAGGCCTACC TGCGTGAAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of apoptotic response by LAR family phosphatases-cIAP1 signaling during urinary tract morphogenesis.
Proceedings of the National Academy of Sciences of the United States of America114(43)E9016-E9025(2017 Oct)
Stewart K,Tang YC,Shafer MER,Graham-Paquin AL,Bouchard M

BID-dependent release of mitochondrial SMAC dampens XIAP-mediated immunity against Shigella.
The EMBO journal33(19)2171-87(2014 Oct)
Andree M,Seeger JM,Sch?ll S,Coutelle O,Wagner-Stippich D,Wiegmann K,Wunderlich CM,Brinkmann K,Broxtermann P,Witt A,Fritsch M,Martinelli P,Bielig H,Lamkemeyer T,Rugarli EI,Kaufmann T,Sterner-Kock A,Wunderlich FT,Villunger A,Martins LM,Kr?nke M,Kufer TA,Uterm?hlen O,Kashkar H

Smac-mimetic-induced epithelial cell death reduces the growth of renal cysts.
Journal of the American Society of Nephrology : JASN24(12)2010-22(2013 Dec)
Fan LX,Zhou X,Sweeney WE Jr,Wallace DP,Avner ED,Grantham JJ,Li X

Identification of a novel anti-apoptotic E3 ubiquitin ligase that ubiquitinates antagonists of inhibitor of apoptosis proteins SMAC, HtrA2, and ARTS.
The Journal of biological chemistry288(17)12014-21(2013 Apr)
Kim JB,Kim SY,Kim BM,Lee H,Kim I,Yun J,Jo Y,Oh T,Jo Y,Chae HD,Shin DY

Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis.
Biochemical and biophysical research communications319(2)649-54(2004 Jun)
Yoon K,Jang HD,Lee SY