Mis18a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mis18a
Entrez Gene ID	66578
Full Name	MIS18 kinetochore protein A
Synonyms	2610039C10Rik,2810018N07Rik
General protein information	Preferred Names MIS18 kinetochore protein A Names protein Mis18-alpha MIS18 kinetochore protein homolog A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_025642.1	NP_079918.1	protein Mis18-alpha

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mis18a	NP_001120995.2
Xenopus tropicalis (tropical clawed frog)	mis18a	XP_002938488.2
Homo sapiens (human)	MIS18A	NP_061817.1
Canis lupus familiaris (dog)	MIS18A	XP_852107.1
Bos taurus (cattle)	MIS18A	NP_001091479.1
Mus musculus (house mouse)	Mis18a	NP_079918.1
Danio rerio (zebrafish)	mis18a	XP_002663634.2
	LOC709275	XP_001097780.2
Pan troglodytes (chimpanzee)	MIS18A	NP_001065279.1
Gallus gallus (chicken)	MIS18A	XP_416702.1

Gene Ontology
Bibliography

Related articles in PubMed

Skin-Specific Deletion of Mis18? Impedes Proliferation and Stratification of Epidermal?Keratinocytes.
Park KC, Lee M, Jeon Y, Jeon R, Baek SH, Lee H, Kim KI
The Journal of investigative dermatology137(2)414-421(2017 Feb)

Roles of Mis18? in epigenetic regulation of centromeric chromatin and CENP-A loading.
Kim IS, Lee M, Park KC, Jeon Y, Park JH, Hwang EJ, Jeon TI, Ko S, Lee H, Baek SH, Kim KI
Molecular cell46(3)260-73(2012 May)

Regulatory pathway analysis by high-throughput in situ hybridization.
Visel A, Carson J, Oldekamp J, Warnecke M, Jakubcakova V, Zhou X, Shaw CA, Alvarez-Bolado G, Eichele G
PLoS genetics3(10)1867-83(2007 Oct)

A gene expression map of human chromosome 21 orthologues in the mouse.
Gitton Y, Dahmane N, Baik S, Ruiz i Altaba A, Neidhardt L, Scholze M, Herrmann BG, Kahlem P, Benkahla A, Schrinner S, Yildirimman R, Herwig R, Lehrach H, Yaspo ML,
Nature420(6915)586-90(2002 Dec)

Human chromosome 21 gene expression atlas in the mouse.
Reymond A, Marigo V, Yaylaoglu MB, Leoni A, Ucla C, Scamuffa N, Caccioppoli C, Dermitzakis ET, Lyle R, Banfi S, Eichele G, Antonarakis SE, Ballabio A
Nature420(6915)582-6(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mis18alpha is important for epidermal cell proliferation and stratification, because it is required for the deposition of CENP-A at the centromeric nucleosomes.
Title: Skin-Specific Deletion of Mis18? Impedes Proliferation and Stratification of Epidermal?Keratinocytes.

Mis18alpha interacts with DNMT3A/3B, and this interaction is critical for maintaining DNA methylation and hence regulating epigenetic states of centromeric chromatin.
Title: Roles of Mis18? in epigenetic regulation of centromeric chromatin and CENP-A loading.

The following Mis18a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mis18a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00809	NM_025642.1 Latest version!	Mus musculus MIS18 kinetochore protein A (Mis18a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mis18a ( NM_025642.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079918.1 Mus musculus MIS18 kinetochore protein A (Mis18a), mRNA.

Price & Availability↑

CloneID	OMu00809
Clone ID Related Accession (Same CDS sequence)	NM_025642.1
Accession Version	NM_025642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	protein Mis18-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK012533.1. On Jul 17, 2005 this sequence version replaced XM_619410.1. ##Evidence-Data-START## Transcript exon combination :: AK012533.1, AK163310.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAGCG AGTCCCCCCT GCTGGAGAAG CGTCTGTCGG AAGACTCGAG CCGCTACCTG 
CGGCTGCAGA AGTGGGCAAA CATGTCGAGC GCAGACGCGT TAGGGCTAGA GAAGGAACGG 
CCTGAGGAGA AGGCGGCCGC GGCGGAGAAC CCGCTGGTGT TCCTTTGTGC CCGCTGTCGC 
CGGCCACTGG GCGACTCGCT CACCTGGGTG GCCAGCCAGG AGGACACCAA CTGCATCCTG 
TTGCGCAGCG TCTCCTGTAA CGTCTCTGTG GATAAGGAAC CGAAACTGTC CAAGTGCAGA 
GATGAAGACG GTTGCATCCT TGAGGCGCTG TACTGCACGG GCTGCTCCCT CAGCCTTGGC 
TATGTGTACA GATGCACTCC TAAGAACCTG GACTACAAGC GCGACTTGTT CTGCCTCAGC 
GTTGAAGCCG TTGAAAGTTA CACCTTAGGG TCCTCTGAGA AGCAAATCGT GTCAGAAGAC 
AAGGAGCTTT TCAACCTTGA AAGCCGAGTT GAAATAGAGA AGTCTATAAA GCAGATGGAA 
GAAGTCCTGA CAGCCCTGCA AAAGAAGCTA CGGGAAGTTG AATCTAAACT GTCCTTGGCC 
AGCCGGGGCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079918.1
CDS	100..714
Translation	MSSESPLLEKRLSEDSSRYLRLQKWANMSSADALGLEKERPEEKAAAAENPLVFLCARCRRPLGDSLTWVASQEDTNCILLRSVSCNVSVDKEPKLSKCRDEDGCILEALYCTGCSLSLGYVYRCTPKNLDYKRDLFCLSVEAVESYTLGSSEKQIVSEDKELFNLESRVEIEKSIKQMEEVLTALQKKLREVESKLSLASRGS

Target ORF information:

RefSeq Version	NM_025642.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MIS18 kinetochore protein A (Mis18a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025642.1

ORF Insert Sequence:

ATGTCGAGCG AGTCCCCCCT GCTGGAGAAG CGTCTGTCGG AAGACTCGAG CCGCTACCTG 
CGGCTGCAGA AGTGGGCAAA CATGTCGAGC GCAGACGCGT TAGGGCTAGA GAAGGAACGG 
CCTGAGGAGA AGGCGGCCGC GGCGGAGAAC CCGCTGGTGT TCCTTTGTGC CCGCTGTCGC 
CGGCCACTGG GCGACTCGCT CACCTGGGTG GCCAGCCAGG AGGACACCAA CTGCATCCTG 
TTGCGCAGCG TCTCCTGTAA CGTCTCTGTG GATAAGGAAC CGAAACTGTC CAAGTGCAGA 
GATGAAGACG GTTGCATCCT TGAGGCGCTG TACTGCACGG GCTGCTCCCT CAGCCTTGGC 
TATGTGTACA GATGCACTCC TAAGAACCTG GACTACAAGC GCGACTTGTT CTGCCTCAGC 
GTTGAAGCCG TTGAAAGTTA CACCTTAGGG TCCTCTGAGA AGCAAATCGT GTCAGAAGAC 
AAGGAGCTTT TCAACCTTGA AAGCCGAGTT GAAATAGAGA AGTCTATAAA GCAGATGGAA 
GAAGTCCTGA CAGCCCTGCA AAAGAAGCTA CGGGAAGTTG AATCTAAACT GTCCTTGGCC 
AGCCGGGGCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Skin-Specific Deletion of Mis18? Impedes Proliferation and Stratification of Epidermal?Keratinocytes.
The Journal of investigative dermatology137(2)414-421(2017 Feb)
Park KC,Lee M,Jeon Y,Jeon R,Baek SH,Lee H,Kim KI

Roles of Mis18? in epigenetic regulation of centromeric chromatin and CENP-A loading.
Molecular cell46(3)260-73(2012 May)
Kim IS,Lee M,Park KC,Jeon Y,Park JH,Hwang EJ,Jeon TI,Ko S,Lee H,Baek SH,Kim KI

Regulatory pathway analysis by high-throughput in situ hybridization.
PLoS genetics3(10)1867-83(2007 Oct)
Visel A,Carson J,Oldekamp J,Warnecke M,Jakubcakova V,Zhou X,Shaw CA,Alvarez-Bolado G,Eichele G

A gene expression map of human chromosome 21 orthologues in the mouse.
Nature420(6915)586-90(2002 Dec)
Gitton Y,Dahmane N,Baik S,Ruiz i Altaba A,Neidhardt L,Scholze M,Herrmann BG,Kahlem P,Benkahla A,Schrinner S,Yildirimman R,Herwig R,Lehrach H,Yaspo ML,

Human chromosome 21 gene expression atlas in the mouse.
Nature420(6915)582-6(2002 Dec)
Reymond A,Marigo V,Yaylaoglu MB,Leoni A,Ucla C,Scamuffa N,Caccioppoli C,Dermitzakis ET,Lyle R,Banfi S,Eichele G,Antonarakis SE,Ballabio A

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Mis18a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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