Timm50 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Timm50
Entrez Gene ID	66525
Full Name	translocase of inner mitochondrial membrane 50
Synonyms	2810403L02Rik,AU015082,TIM50L
General protein information	Preferred Names translocase of inner mitochondrial membrane 50 Names mitochondrial import inner membrane translocase subunit TIM50 translocase of inner mitochondrial membrane 50 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011250673.2	XP_011248975.1	mitochondrial import inner membrane translocase subunit TIM50 isoform X1
XM_011250673.3	XP_011248975.1	mitochondrial import inner membrane translocase subunit TIM50 isoform X1
NM_025616.3	NP_079892.1	mitochondrial import inner membrane translocase subunit TIM50 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TIMM50	XP_001138592.1
Bos taurus (cattle)	TIMM50	NP_001030196.1
Mus musculus (house mouse)	Timm50	NP_079892.1
Rattus norvegicus (Norway rat)	LOC687295	XP_003748854.1
Xenopus tropicalis (tropical clawed frog)	timm50	NP_001120038.1
Gallus gallus (chicken)	TIMM50	XP_001233751.3
Danio rerio (zebrafish)	timm50	NP_956959.1
Drosophila melanogaster (fruit fly)	ttm50	NP_570027.1
Homo sapiens (human)	TIMM50	NP_001001563.1
Canis lupus familiaris (dog)	TIMM50	XP_533672.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001108	XP_322056.5

Gene Ontology
Bibliography

Related articles in PubMed

Translocase of Inner Membrane 50 Functions as a Novel Protective Regulator of Pathological Cardiac Hypertrophy.
Tang K, Zhao Y, Li H, Zhu M, Li W, Liu W, Zhu G, Xu D, Peng W, Xu YW
Journal of the American Heart Association6(4)(2017 Apr)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death.
Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber SA, Fernandes-Alnemri T, Alnemri ES
The Journal of biological chemistry279(23)24813-25(2004 Jun)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Timm50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Timm50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13400	NM_025616.3 Latest version!	Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu69958	XM_011250673.3 Latest version!	Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu69958	XM_011250673.2	Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Timm50 ( NM_025616.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079892.1 Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), mRNA.

Price & Availability↑

CloneID	OMu13400
Clone ID Related Accession (Same CDS sequence)	NM_025616.3
Accession Version	NM_025616.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	mitochondrial import inner membrane translocase subunit TIM50 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC148986.5. On Apr 29, 2007 this sequence version replaced NM_025616.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK008340.1, AK167473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGGCCT CGGCGGCTCT GTTCTCGCGC TTGCGGAGCG GGCTCCGGGT AGGCGCGCGG 
GGGCTGTGCA CGCGGCTAGC GCCGCCACCT CCCCGCACTC CCGAGCAGGT CACTGAAATT 
GCCAACCGTG GGGGCTCCAA GGCCCAGGGG CCACAGCACC AGCCAGGCTC CGAGGGTCCC 
AGCTATGCCA AAAAAATTGC GCTCTGGATC GCCGGTTTGC TCGGAGCTGG TGGGACTGTC 
AGCATCGTCT ATATTTTTGG AAACAACCCT GTGGATGAAA ATGGTACCAA GATTCCTGAT 
GAATTCGACA GCGATCCAAT TCTGGTTCAG CAGTTGCGCC GGACATACAA ATACTTCAAA 
GATTACAGAC AGATGATCAT CGAGCCCACC AGTCCCTGCC TTCTCCCAGA CCCTCTGCGG 
GAGCCCTACT ACCAGCCACC TTATACACTG GTCCTGGAGC TTACCGGGGT CCTTCTGCAC 
CCAGAGTGGT CGCTGGCCAC TGGCTGGAGG TTTAAGAAGC GTCCAGGCAT CGAGACCTTG 
TTCCAGCAGC TTGCCCCTCT GTATGAAATA GTCATCTTCA CATCAGAGAC TGGCATGACT 
GCATTTCCAC TCATTGATAG TGTGGACCCT CACGGTTTCA TCTCCTACCG TCTGTTCCGG 
GACGCCACCA GATATATGGA GGGACATCAT GTGAAGGACA TCTCCTGTCT GAATCGGGAC 
CCAGCCCGAG TAGTAGTCGT GGACTGCAAG AAGGAAGCTT TCCGCCTCCA GCCCTTCAAC 
GGTGTTGCCC TGCGGCCCTG GGATGGCAAC TCAGATGACC GGGTCTTGCT GGACCTGTCT 
GCCTTCCTCA AGACCATTGC ACTGAACCAA GTGGAGGACG TCCGGACGGT GCTGGAGCAC 
TATGCTCTAG AGGATGACCC ACTGGAGGCA TTCAAACAGC GGCAGAGCCG GCTTGAGCAG 
GAGGAGCAGC AGCGCCTGGC CGAGCTCTCC AAGTCCAACA GGCAGGGCCT CTCCTTTGGC 
TCACTCGCCA GCCGACTGTG GCCTCGCTCC AAGCAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079892.1
CDS	15..1076
Translation	MAASAALFSRLRSGLRVGARGLCTRLAPPPPRTPEQVTEIANRGGSKAQGPQHQPGSEGPSYAKKIALWIAGLLGAGGTVSIVYIFGNNPVDENGTKIPDEFDSDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVALRPWDGNSDDRVLLDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNRQGLSFGSLASRLWPRSKQP

Target ORF information:

RefSeq Version	NM_025616.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025616.3

ORF Insert Sequence:

ATGGCGGCCT CGGCGGCTCT GTTCTCGCGC TTGCGGAGCG GGCTCCGGGT AGGCGCGCGG 
GGGCTGTGCA CGCGGCTAGC GCCGCCACCT CCCCGCACTC CCGAGCAGGT CACTGAAATT 
GCCAACCGTG GGGGCTCCAA GGCCCAGGGG CCACAGCACC AGCCAGGCTC CGAGGGTCCC 
AGCTATGCCA AAAAAATTGC GCTCTGGATC GCCGGTTTGC TCGGAGCTGG TGGGACTGTC 
AGCATCGTCT ATATTTTTGG AAACAACCCT GTGGATGAAA ATGGTACCAA GATTCCTGAT 
GAATTCGACA GCGATCCAAT TCTGGTTCAG CAGTTGCGCC GGACATACAA ATACTTCAAA 
GATTACAGAC AGATGATCAT CGAGCCCACC AGTCCCTGCC TTCTCCCAGA CCCTCTGCGG 
GAGCCCTACT ACCAGCCACC TTATACACTG GTCCTGGAGC TTACCGGGGT CCTTCTGCAC 
CCAGAGTGGT CGCTGGCCAC TGGCTGGAGG TTTAAGAAGC GTCCAGGCAT CGAGACCTTG 
TTCCAGCAGC TTGCCCCTCT GTATGAAATA GTCATCTTCA CATCAGAGAC TGGCATGACT 
GCATTTCCAC TCATTGATAG TGTGGACCCT CACGGTTTCA TCTCCTACCG TCTGTTCCGG 
GACGCCACCA GATATATGGA GGGACATCAT GTGAAGGACA TCTCCTGTCT GAATCGGGAC 
CCAGCCCGAG TAGTAGTCGT GGACTGCAAG AAGGAAGCTT TCCGCCTCCA GCCCTTCAAC 
GGTGTTGCCC TGCGGCCCTG GGATGGCAAC TCAGATGACC GGGTCTTGCT GGACCTGTCT 
GCCTTCCTCA AGACCATTGC ACTGAACCAA GTGGAGGACG TCCGGACGGT GCTGGAGCAC 
TATGCTCTAG AGGATGACCC ACTGGAGGCA TTCAAACAGC GGCAGAGCCG GCTTGAGCAG 
GAGGAGCAGC AGCGCCTGGC CGAGCTCTCC AAGTCCAACA GGCAGGGCCT CTCCTTTGGC 
TCACTCGCCA GCCGACTGTG GCCTCGCTCC AAGCAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timm50 ( XM_011250673.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248975.1 Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69958
Clone ID Related Accession (Same CDS sequence)	XM_011250673.2 , XM_011250673.3
Accession Version	XM_011250673.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitochondrial import inner membrane translocase subunit TIM50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGGA GCCCGCGTTT TCGATGTTTC CATGCTTCCT GTGTGGACCA CAGTTGCTCC 
CCCGGGGCTG GATTATGGAG GCCCGGAGTG CATCCCCTGG AGCCCAGGTC TCAACCACGT 
GGGTGCCGGG AGAGGTCACC TGTTCTGATC TCGGTGACCA TTCAGGTCAC TGAAATTGCC 
AACCGTGGGG GCTCCAAGGC CCAGGGGCCA CAGCACCAGC CAGGCTCCGA GGGTCCCAGC 
TATGCCAAAA AAATTGCGCT CTGGATCGCC GGTTTGCTCG GAGCTGGTGG GACTGTCAGC 
ATCGTCTATA TTTTTGGAAA CAACCCTGTG GATGAAAATG GTACCAAGAT TCCTGATGAA 
TTCGACAGCG ATCCAATTCT GGTTCAGCAG TTGCGCCGGA CATACAAATA CTTCAAAGAT 
TACAGACAGA TGATCATCGA GCCCACCAGT CCCTGCCTTC TCCCAGACCC TCTGCGGGAG 
CCCTACTACC AGCCACCTTA TACACTGGTC CTGGAGCTTA CCGGGGTCCT TCTGCACCCA 
GAGTGGTCGC TGGCCACTGG CTGGAGGTTT AAGAAGCGTC CAGGCATCGA GACCTTGTTC 
CAGCAGCTTG CCCCTCTGTA TGAAATAGTC ATCTTCACAT CAGAGACTGG CATGACTGCA 
TTTCCACTCA TTGATAGTGT GGACCCTCAC GGTTTCATCT CCTACCGTCT GTTCCGGGAC 
GCCACCAGAT ATATGGAGGG ACATCATGTG AAGGACATCT CCTGTCTGAA TCGGGACCCA 
GCCCGAGTAG TAGTCGTGGA CTGCAAGAAG GAAGCTTTCC GCCTCCAGCC CTTCAACGGT 
GTTGCCCTGC GGCCCTGGGA TGGCAACTCA GATGACCGGG TCTTGCTGGA CCTGTCTGCC 
TTCCTCAAGA CCATTGCACT GAACCAAGTG GAGGACGTCC GGACGGTGCT GGAGCACTAT 
GCTCTAGAGG ATGACCCACT GGAGGCATTC AAACAGCGGC AGAGCCGGCT TGAGCAGGAG 
GAGCAGCAGC GCCTGGCCGA GCTCTCCAAG TCCAACAGGC AGGGCCTCTC CTTTGGCTCA 
CTCGCCAGCC GACTGTGGCC TCGCTCCAAG CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248975.1
CDS	68..1186
Translation	MGRSPRFRCFHASCVDHSCSPGAGLWRPGVHPLEPRSQPRGCRERSPVLISVTIQVTEIANRGGSKAQGPQHQPGSEGPSYAKKIALWIAGLLGAGGTVSIVYIFGNNPVDENGTKIPDEFDSDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVALRPWDGNSDDRVLLDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNRQGLSFGSLASRLWPRSKQP

Target ORF information:

RefSeq Version	XM_011250673.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250673.2

ORF Insert Sequence:

ATGGGACGGA GCCCGCGTTT TCGATGTTTC CATGCTTCCT GTGTGGACCA CAGTTGCTCC 
CCCGGGGCTG GATTATGGAG GCCCGGAGTG CATCCCCTGG AGCCCAGGTC TCAACCACGT 
GGGTGCCGGG AGAGGTCACC TGTTCTGATC TCGGTGACCA TTCAGGTCAC TGAAATTGCC 
AACCGTGGGG GCTCCAAGGC CCAGGGGCCA CAGCACCAGC CAGGCTCCGA GGGTCCCAGC 
TATGCCAAAA AAATTGCGCT CTGGATCGCC GGTTTGCTCG GAGCTGGTGG GACTGTCAGC 
ATCGTCTATA TTTTTGGAAA CAACCCTGTG GATGAAAATG GTACCAAGAT TCCTGATGAA 
TTCGACAGCG ATCCAATTCT GGTTCAGCAG TTGCGCCGGA CATACAAATA CTTCAAAGAT 
TACAGACAGA TGATCATCGA GCCCACCAGT CCCTGCCTTC TCCCAGACCC TCTGCGGGAG 
CCCTACTACC AGCCACCTTA TACACTGGTC CTGGAGCTTA CCGGGGTCCT TCTGCACCCA 
GAGTGGTCGC TGGCCACTGG CTGGAGGTTT AAGAAGCGTC CAGGCATCGA GACCTTGTTC 
CAGCAGCTTG CCCCTCTGTA TGAAATAGTC ATCTTCACAT CAGAGACTGG CATGACTGCA 
TTTCCACTCA TTGATAGTGT GGACCCTCAC GGTTTCATCT CCTACCGTCT GTTCCGGGAC 
GCCACCAGAT ATATGGAGGG ACATCATGTG AAGGACATCT CCTGTCTGAA TCGGGACCCA 
GCCCGAGTAG TAGTCGTGGA CTGCAAGAAG GAAGCTTTCC GCCTCCAGCC CTTCAACGGT 
GTTGCCCTGC GGCCCTGGGA TGGCAACTCA GATGACCGGG TCTTGCTGGA CCTGTCTGCC 
TTCCTCAAGA CCATTGCACT GAACCAAGTG GAGGACGTCC GGACGGTGCT GGAGCACTAT 
GCTCTAGAGG ATGACCCACT GGAGGCATTC AAACAGCGGC AGAGCCGGCT TGAGCAGGAG 
GAGCAGCAGC GCCTGGCCGA GCTCTCCAAG TCCAACAGGC AGGGCCTCTC CTTTGGCTCA 
CTCGCCAGCC GACTGTGGCC TCGCTCCAAG CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timm50 ( XM_011250673.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248975.1 Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69958
Clone ID Related Accession (Same CDS sequence)	XM_011250673.2 , XM_011250673.3
Accession Version	XM_011250673.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitochondrial import inner membrane translocase subunit TIM50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGGA GCCCGCGTTT TCGATGTTTC CATGCTTCCT GTGTGGACCA CAGTTGCTCC 
CCCGGGGCTG GATTATGGAG GCCCGGAGTG CATCCCCTGG AGCCCAGGTC TCAACCACGT 
GGGTGCCGGG AGAGGTCACC TGTTCTGATC TCGGTGACCA TTCAGGTCAC TGAAATTGCC 
AACCGTGGGG GCTCCAAGGC CCAGGGGCCA CAGCACCAGC CAGGCTCCGA GGGTCCCAGC 
TATGCCAAAA AAATTGCGCT CTGGATCGCC GGTTTGCTCG GAGCTGGTGG GACTGTCAGC 
ATCGTCTATA TTTTTGGAAA CAACCCTGTG GATGAAAATG GTACCAAGAT TCCTGATGAA 
TTCGACAGCG ATCCAATTCT GGTTCAGCAG TTGCGCCGGA CATACAAATA CTTCAAAGAT 
TACAGACAGA TGATCATCGA GCCCACCAGT CCCTGCCTTC TCCCAGACCC TCTGCGGGAG 
CCCTACTACC AGCCACCTTA TACACTGGTC CTGGAGCTTA CCGGGGTCCT TCTGCACCCA 
GAGTGGTCGC TGGCCACTGG CTGGAGGTTT AAGAAGCGTC CAGGCATCGA GACCTTGTTC 
CAGCAGCTTG CCCCTCTGTA TGAAATAGTC ATCTTCACAT CAGAGACTGG CATGACTGCA 
TTTCCACTCA TTGATAGTGT GGACCCTCAC GGTTTCATCT CCTACCGTCT GTTCCGGGAC 
GCCACCAGAT ATATGGAGGG ACATCATGTG AAGGACATCT CCTGTCTGAA TCGGGACCCA 
GCCCGAGTAG TAGTCGTGGA CTGCAAGAAG GAAGCTTTCC GCCTCCAGCC CTTCAACGGT 
GTTGCCCTGC GGCCCTGGGA TGGCAACTCA GATGACCGGG TCTTGCTGGA CCTGTCTGCC 
TTCCTCAAGA CCATTGCACT GAACCAAGTG GAGGACGTCC GGACGGTGCT GGAGCACTAT 
GCTCTAGAGG ATGACCCACT GGAGGCATTC AAACAGCGGC AGAGCCGGCT TGAGCAGGAG 
GAGCAGCAGC GCCTGGCCGA GCTCTCCAAG TCCAACAGGC AGGGCCTCTC CTTTGGCTCA 
CTCGCCAGCC GACTGTGGCC TCGCTCCAAG CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248975.1
CDS	193..1311
Translation	MGRSPRFRCFHASCVDHSCSPGAGLWRPGVHPLEPRSQPRGCRERSPVLISVTIQVTEIANRGGSKAQGPQHQPGSEGPSYAKKIALWIAGLLGAGGTVSIVYIFGNNPVDENGTKIPDEFDSDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVALRPWDGNSDDRVLLDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNRQGLSFGSLASRLWPRSKQP

Target ORF information:

RefSeq Version	XM_011250673.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus translocase of inner mitochondrial membrane 50 (Timm50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250673.3

ORF Insert Sequence:

ATGGGACGGA GCCCGCGTTT TCGATGTTTC CATGCTTCCT GTGTGGACCA CAGTTGCTCC 
CCCGGGGCTG GATTATGGAG GCCCGGAGTG CATCCCCTGG AGCCCAGGTC TCAACCACGT 
GGGTGCCGGG AGAGGTCACC TGTTCTGATC TCGGTGACCA TTCAGGTCAC TGAAATTGCC 
AACCGTGGGG GCTCCAAGGC CCAGGGGCCA CAGCACCAGC CAGGCTCCGA GGGTCCCAGC 
TATGCCAAAA AAATTGCGCT CTGGATCGCC GGTTTGCTCG GAGCTGGTGG GACTGTCAGC 
ATCGTCTATA TTTTTGGAAA CAACCCTGTG GATGAAAATG GTACCAAGAT TCCTGATGAA 
TTCGACAGCG ATCCAATTCT GGTTCAGCAG TTGCGCCGGA CATACAAATA CTTCAAAGAT 
TACAGACAGA TGATCATCGA GCCCACCAGT CCCTGCCTTC TCCCAGACCC TCTGCGGGAG 
CCCTACTACC AGCCACCTTA TACACTGGTC CTGGAGCTTA CCGGGGTCCT TCTGCACCCA 
GAGTGGTCGC TGGCCACTGG CTGGAGGTTT AAGAAGCGTC CAGGCATCGA GACCTTGTTC 
CAGCAGCTTG CCCCTCTGTA TGAAATAGTC ATCTTCACAT CAGAGACTGG CATGACTGCA 
TTTCCACTCA TTGATAGTGT GGACCCTCAC GGTTTCATCT CCTACCGTCT GTTCCGGGAC 
GCCACCAGAT ATATGGAGGG ACATCATGTG AAGGACATCT CCTGTCTGAA TCGGGACCCA 
GCCCGAGTAG TAGTCGTGGA CTGCAAGAAG GAAGCTTTCC GCCTCCAGCC CTTCAACGGT 
GTTGCCCTGC GGCCCTGGGA TGGCAACTCA GATGACCGGG TCTTGCTGGA CCTGTCTGCC 
TTCCTCAAGA CCATTGCACT GAACCAAGTG GAGGACGTCC GGACGGTGCT GGAGCACTAT 
GCTCTAGAGG ATGACCCACT GGAGGCATTC AAACAGCGGC AGAGCCGGCT TGAGCAGGAG 
GAGCAGCAGC GCCTGGCCGA GCTCTCCAAG TCCAACAGGC AGGGCCTCTC CTTTGGCTCA 
CTCGCCAGCC GACTGTGGCC TCGCTCCAAG CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Translocase of Inner Membrane 50 Functions as a Novel Protective Regulator of Pathological Cardiac Hypertrophy.
Journal of the American Heart Association6(4)(2017 Apr)
Tang K,Zhao Y,Li H,Zhu M,Li W,Liu W,Zhu G,Xu D,Peng W,Xu YW

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
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Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death.
The Journal of biological chemistry279(23)24813-25(2004 Jun)
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Timm50 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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