Ctcfl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctcfl
Entrez Gene ID	664799
Full Name	CCCTC-binding factor (zinc finger protein)-like
Synonyms	Boris
General protein information	Preferred Names CCCTC-binding factor (zinc finger protein)-like Names transcriptional repressor CTCFL BORIS Brother of the regulator of imprinted sites CTCF paralog CTCF-like protein likely orthologue of H. sapiens CCCTC-binding factor-like protein (CTCFL)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081387.2	NP_001074856.1	transcriptional repressor CTCFL isoform 2
XM_017319167.1	XP_017174656.1	transcriptional repressor CTCFL isoform X1
XM_017319167.2	XP_017174656.1	transcriptional repressor CTCFL isoform X1
XM_017319168.1	XP_017174657.1	transcriptional repressor CTCFL isoform X2
NM_001355185.1	NP_001342114.1	transcriptional repressor CTCFL isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CTCFL	XP_003953828.1
Bos taurus (cattle)	CTCFL	NP_001116837.1
Mus musculus (house mouse)	Ctcfl	NP_001074856.1
Homo sapiens (human)	CTCFL	NP_001255972.1
	CTCFL	XP_001089797.1
Canis lupus familiaris (dog)	CTCFL	XP_534463.2
Rattus norvegicus (Norway rat)	LOC100360757	XP_002729301.1

Gene Ontology
Bibliography

Related articles in PubMed

Testis-specific transcriptional regulators selectively occupy BORIS-bound CTCF target regions in mouse male germ cells.
Rivero-Hinojosa S, Kang S, Lobanenkov VV, Zentner GE
Scientific reports741279(2017 Feb)

Expression of the CTCFL Gene during Mouse Embryogenesis Causes Growth Retardation, Postnatal Lethality, and Dysregulation of the Transforming Growth Factor ? Pathway.
Sati L, Zeiss C, Yekkala K, Demir R, McGrath J
Molecular and cellular biology35(19)3436-45(2015 Oct)

The male germ cell gene regulator CTCFL is functionally different from CTCF and binds CTCF-like consensus sites in a nucleosome composition-dependent manner.
Sleutels F, Soochit W, Bartkuhn M, Heath H, Dienstbach S, Bergmaier P, Franke V, Rosa-Garrido M, van de Nobelen S, Caesar L, van der Reijden M, Bryne JC, van Ijcken W, Grootegoed JA, Delgado MD, Lenhard B, Renkawitz R, Grosveld F, Galjart N
Epigenetics & chromatin5(1)8(2012 Jun)

Integrating role of T antigen, Rb2/p130, CTCF and BORIS in mediating non-canonical endoplasmic reticulum-dependent death pathways triggered by chronic ER stress in mouse medulloblastoma.
Macaluso M, Caracciolo V, Rizzo V, Sun A, Montanari M, Russo G, Bellipanni G, Khalili K, Giordano A
Cell cycle (Georgetown, Tex.)11(9)1841-50(2012 May)

A cell cycle role for the epigenetic factor CTCF-L/BORIS.
Rosa-Garrido M, Ceballos L, Alonso-Lecue P, Abraira C, Delgado MD, Gandarillas A
PloS one7(6)e39371(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The TGFB pathway as most affected by embryonic Ctcfl expression.
Title: Expression of the CTCFL Gene during Mouse Embryogenesis Causes Growth Retardation, Postnatal Lethality, and Dysregulation of the Transforming Growth Factor ? Pathway.

Rb2 coimmunolocalizes with the chromatin insulator CCCTC-binding factor (CTCF) and BORIS in T-antigen-positive but not in T-antigen-negative cells.
Title: Integrating role of T antigen, Rb2/p130, CTCF and BORIS in mediating non-canonical endoplasmic reticulum-dependent death pathways triggered by chronic ER stress in mouse medulloblastoma.

down-regulation of endogenous BORIS by specific shRNAs inhibited both RNA transcription and cell cycle progression. The results altogether suggest a role for BORIS in coordinating S phase events with mitosis.
Title: A cell cycle role for the epigenetic factor CTCF-L/BORIS.

findings indicate that BORIS-induced expression of TSP50 is governed by accessibility and binding of BORIS to the promoter
Title: Transcription factor BORIS (Brother of the Regulator of Imprinted Sites) directly induces expression of a cancer-testis antigen, TSP50, through regulated binding of BORIS to the promoter.

These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function.
Title: Widespread expression of BORIS/CTCFL in normal and cancer cells.

The following Ctcfl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctcfl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14818	NM_001081387.2 Latest version!	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu30774	NM_001355185.1 Latest version!	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu30774	XM_017319168.1 Latest version!	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu80366	XM_017319167.2 Latest version!	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu80366	XM_017319167.1	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctcfl ( NM_001081387.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074856.1 Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14818
Clone ID Related Accession (Same CDS sequence)	NM_001081387.2
Accession Version	NM_001081387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-18
Organism	Mus musculus(house mouse)
Product	transcriptional repressor CTCFL isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL837509.11 and AL928599.5. On May 24, 2007 this sequence version replaced NM_001081387.1. Transcript Variant: This variant (2) uses an alternate in-frame 3' splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ153171.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCG CTGAGGTCCC TGTCCCTTCT GGGTACTTCA CCCAGATCAA AGAGCAGAAG 
TTGAAGCCTG GAGACCTAGA GGAGGAGAAA GAGGAGGACG GGGTACAAAG AGTGGAAGCC 
CAGGAGGGAG TTGTCAAGGA GGTGGAGGCC GAGAACAGTT GCCTGCTTCT GGAGGCCAGG 
GCCCCGGTGG AGAGCGACAG GCGGATCCTG ACCCTGCAAA CGGTGCACCT GGAGTCCCAG 
GATGTGCACC TACAGGGGCT GGGATGGCTG AGCGTGCCAC ACTCTGAGGA GCTTTCAGGG 
ACGGTACCAG AGGCGGAAGG CATACTGCAG TTGCCATCCG TGCTGTGGCT CGACCCAGAG 
CCCCAGCTCA GCCTTCAGCA TTGCGTGACG GTCAGCATCC CGGAAGAGCT GTACCCACCA 
GAGGAGCTGC AGCGGATACA TTTTCACCTG CTGAGAGAGA ATGTGCTAAT GGCCGAGGAG 
AACCCAGAGT TAACACCAGA CTTGGACGAA AGCACAGCCC TGAAAAAGCC CGAAGAAGAT 
GAAAAGGACC AGCTCCCGCC CCAGGGAGAG ACAGACAAGA GAGAAGAGAG GTTGCTCCTT 
CTGGAAATGA AACCAAAAGA GGGAAAAGAC GACGAAATTG TCCTGACCAT TTCCCATCTA 
AGCCTCGAAG AACAGCAAGA TCCACCAGCG GCCAATCAGA CAAGTGTGCC GGGAGCCAAA 
GCCGCAAAAC CAAAACGGCG GAGGCAGACC AAGGGAAAGC CTCAGAGCTT TCAGTGTGAC 
ACCTGCCCGT TCACTTCCTC CAAGCTCTCA ACTTTCAATC GTCACATCAA AATTCACAGC 
AATGAGAGGC CACACCTGTG TCACCTGTGC CTGAAGGCCT TCCGGACTGT CACTCTTCTT 
AGGAACCATG TGAACACCCA CACAGGAACC AGGCCCCACA AGTGCAGGGA CTGCGACATG 
GCGTTTGTCA CCAGTGGAGA ACTCGTCCGG CACAGGCGTT ACAAACACAC TTATGAGAAG 
CCCTTCAAGT GCTCCCTGTG CAAGTACGCC AGCGTCGAGG CAAGCAAGAT GAAGCGTCAC 
ATCCGCTCAC ACACGGGTGA GCGTCCCTTC CAGTGTTGCC AGTGTGCTTA CGCCAGCAGG 
GACTCCTACA AGCTGAAGCG CCACATGAGG ACACACTCAG GTGAGAAGCC GTATGAATGT 
CCCACCTGTC ACGTCCGGTT CACCCAGAGC GGGACCATGA AAATCCATAT AGCACAGAAG 
CACGGAGAGA ATGTGCCCAA ATACGAGTGT CCCCACTGTG CCACCATCAT CGCGAGGAAG 
AGCGACCTGC GTGTCCATCT GCGTAACCTG CACAGCCAGA GCCCGGAGGA GATGAAGTGC 
CGATACTGTC CCGCTGGCTT CCATGAGCGC TATGCCCTCA TTCAGCACCA GAGGACCCAC 
AAGAACGAGA AGAAGTTCAA GTGCAAGCAG TGCGATTACG CGTGCAAGCA GGAGCGATGC 
TTGAAGGCGC ACATGCGCAT GCACACAGGA GAGAAGCCCT TCTCCTGCCT GGCCTGCAAC 
AAGCACTTCC GACAGAAGCA GCTACTGACC GTGCACCTGA GGAAGTACCA TGACCCGAAC 
TTCGTCCCCA ATCTGCACCT GTGCCTCAAG TGTGATAAAC GTTTCTCCCG CTGGAGTAAC 
CTGCAGAGAC ACAGAAAGAA GTGTGACCCG GAGCATGAGA CGTTAGCCCC CAACAAGGAC 
AGGAGACCAG TGACAAGGAC ACAGGCCTCG GAGGGAGAAG CAGGACACAA GGAAGGGGAG 
CCTCAGTGCC CTGGGGAGCA GGCTCTGGGC CACCAAGGAG AAGCAGCGGG GAGCCAGAGC 
CCAGACCACG GCCTTACCTG CGAGATAATC TTTAACATGA TGGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074856.1
CDS	249..2159
Translation	MAAAEVPVPSGYFTQIKEQKLKPGDLEEEKEEDGVQRVEAQEGVVKEVEAENSCLLLEARAPVESDRRILTLQTVHLESQDVHLQGLGWLSVPHSEELSGTVPEAEGILQLPSVLWLDPEPQLSLQHCVTVSIPEELYPPEELQRIHFHLLRENVLMAEENPELTPDLDESTALKKPEEDEKDQLPPQGETDKREERLLLLEMKPKEGKDDEIVLTISHLSLEEQQDPPAANQTSVPGAKAAKPKRRRQTKGKPQSFQCDTCPFTSSKLSTFNRHIKIHSNERPHLCHLCLKAFRTVTLLRNHVNTHTGTRPHKCRDCDMAFVTSGELVRHRRYKHTYEKPFKCSLCKYASVEASKMKRHIRSHTGERPFQCCQCAYASRDSYKLKRHMRTHSGEKPYECPTCHVRFTQSGTMKIHIAQKHGENVPKYECPHCATIIARKSDLRVHLRNLHSQSPEEMKCRYCPAGFHERYALIQHQRTHKNEKKFKCKQCDYACKQERCLKAHMRMHTGEKPFSCLACNKHFRQKQLLTVHLRKYHDPNFVPNLHLCLKCDKRFSRWSNLQRHRKKCDPEHETLAPNKDRRPVTRTQASEGEAGHKEGEPQCPGEQALGHQGEAAGSQSPDHGLTCEIIFNMMDK

Target ORF information:

RefSeq Version	NM_001081387.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081387.2

ORF Insert Sequence:

ATGGCTGCCG CTGAGGTCCC TGTCCCTTCT GGGTACTTCA CCCAGATCAA AGAGCAGAAG 
TTGAAGCCTG GAGACCTAGA GGAGGAGAAA GAGGAGGACG GGGTACAAAG AGTGGAAGCC 
CAGGAGGGAG TTGTCAAGGA GGTGGAGGCC GAGAACAGTT GCCTGCTTCT GGAGGCCAGG 
GCCCCGGTGG AGAGCGACAG GCGGATCCTG ACCCTGCAAA CGGTGCACCT GGAGTCCCAG 
GATGTGCACC TACAGGGGCT GGGATGGCTG AGCGTGCCAC ACTCTGAGGA GCTTTCAGGG 
ACGGTACCAG AGGCGGAAGG CATACTGCAG TTGCCATCCG TGCTGTGGCT CGACCCAGAG 
CCCCAGCTCA GCCTTCAGCA TTGCGTGACG GTCAGCATCC CGGAAGAGCT GTACCCACCA 
GAGGAGCTGC AGCGGATACA TTTTCACCTG CTGAGAGAGA ATGTGCTAAT GGCCGAGGAG 
AACCCAGAGT TAACACCAGA CTTGGACGAA AGCACAGCCC TGAAAAAGCC CGAAGAAGAT 
GAAAAGGACC AGCTCCCGCC CCAGGGAGAG ACAGACAAGA GAGAAGAGAG GTTGCTCCTT 
CTGGAAATGA AACCAAAAGA GGGAAAAGAC GACGAAATTG TCCTGACCAT TTCCCATCTA 
AGCCTCGAAG AACAGCAAGA TCCACCAGCG GCCAATCAGA CAAGTGTGCC GGGAGCCAAA 
GCCGCAAAAC CAAAACGGCG GAGGCAGACC AAGGGAAAGC CTCAGAGCTT TCAGTGTGAC 
ACCTGCCCGT TCACTTCCTC CAAGCTCTCA ACTTTCAATC GTCACATCAA AATTCACAGC 
AATGAGAGGC CACACCTGTG TCACCTGTGC CTGAAGGCCT TCCGGACTGT CACTCTTCTT 
AGGAACCATG TGAACACCCA CACAGGAACC AGGCCCCACA AGTGCAGGGA CTGCGACATG 
GCGTTTGTCA CCAGTGGAGA ACTCGTCCGG CACAGGCGTT ACAAACACAC TTATGAGAAG 
CCCTTCAAGT GCTCCCTGTG CAAGTACGCC AGCGTCGAGG CAAGCAAGAT GAAGCGTCAC 
ATCCGCTCAC ACACGGGTGA GCGTCCCTTC CAGTGTTGCC AGTGTGCTTA CGCCAGCAGG 
GACTCCTACA AGCTGAAGCG CCACATGAGG ACACACTCAG GTGAGAAGCC GTATGAATGT 
CCCACCTGTC ACGTCCGGTT CACCCAGAGC GGGACCATGA AAATCCATAT AGCACAGAAG 
CACGGAGAGA ATGTGCCCAA ATACGAGTGT CCCCACTGTG CCACCATCAT CGCGAGGAAG 
AGCGACCTGC GTGTCCATCT GCGTAACCTG CACAGCCAGA GCCCGGAGGA GATGAAGTGC 
CGATACTGTC CCGCTGGCTT CCATGAGCGC TATGCCCTCA TTCAGCACCA GAGGACCCAC 
AAGAACGAGA AGAAGTTCAA GTGCAAGCAG TGCGATTACG CGTGCAAGCA GGAGCGATGC 
TTGAAGGCGC ACATGCGCAT GCACACAGGA GAGAAGCCCT TCTCCTGCCT GGCCTGCAAC 
AAGCACTTCC GACAGAAGCA GCTACTGACC GTGCACCTGA GGAAGTACCA TGACCCGAAC 
TTCGTCCCCA ATCTGCACCT GTGCCTCAAG TGTGATAAAC GTTTCTCCCG CTGGAGTAAC 
CTGCAGAGAC ACAGAAAGAA GTGTGACCCG GAGCATGAGA CGTTAGCCCC CAACAAGGAC 
AGGAGACCAG TGACAAGGAC ACAGGCCTCG GAGGGAGAAG CAGGACACAA GGAAGGGGAG 
CCTCAGTGCC CTGGGGAGCA GGCTCTGGGC CACCAAGGAG AAGCAGCGGG GAGCCAGAGC 
CCAGACCACG GCCTTACCTG CGAGATAATC TTTAACATGA TGGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctcfl ( NM_001355185.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342114.1 Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu30774
Clone ID Related Accession (Same CDS sequence)	XM_017319168.1 , NM_001355185.1
Accession Version	NM_001355185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-18
Organism	Mus musculus(house mouse)
Product	transcriptional repressor CTCFL isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL837509.11 and AL928599.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151026.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849384 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCG CTGAGGTCCC TGTCCCTTCT GGGTACTTCA CCCAGATCAA AGAGCAGAAG 
TTGAAGCCTG GAGACCTAGA GGAGGAGAAA GAGGAGGACG GGGTACAAAG AGTGGAAGCC 
CAGGAGGGAG TTGTCAAGGA GGTGGAGGCC GAGAACAGTT GCCTGCTTCT GGAGGCCAGG 
GCCCCGGTGG AGAGCGACAG GCGGATCCTG ACCCTGCAAA CGGTGCACCT GGAGTCCCAG 
GATGTGCACC TACAGGGGCT GGGATGGCTG AGCGTGCCAC ACTCTGAGGA GCTTTCAGGG 
ACGGTACCAG AGGCGGAAGG CATACTGCAG TTGCCATCCG TGCTGTGGCT CGACCCAGAG 
CCCCAGCTCA GCCTTCAGCA TTGCGTGACG GTCAGCATCC CGGAAGAGCT GTACCCACCA 
GAGGAGCTGC AGCGGATACA TTTTCACCTG CTGAGAGAGA ATGTGCTAAT GGCCGAGGAG 
AACCCAGAGT TAACACCAGA CTTGGACGAA AGCACAGCCC TGAAAAAGCC CGAAGAAGAT 
GAAAAGGACC AGCTCCCGCC CCAGGGAGAG ACAGACAAGA GAGAAGAGAG GTTGCTCCTT 
CTGGAAATGA AACCAAAAGA GGGAAAAGAC GACGAAATTG TCCTGACCAT TTCCCATCTA 
AGCCTCGAAG AACAGCAAGA TCCACCAGCG GCCAATCAGA CAAGTGTGCC GGGAGCCAAA 
GCCGCAAAAC CAAAACGGCG GAGGCAGACC AAGGGAAAGC CTCAGAGCTT TCAGTGTGAC 
ACCTGCCCGT TCACTTCCTC CAAGCTCTCA ACTTTCAATC GTCACATCAA AATTCACAGC 
AATGAGAGGC CACACCTGTG TCACCTGTGC CTGAAGGCCT TCCGGACTGT CACTCTTCTT 
AGGAACCATG TGAACACCCA CACAGGAACC AGGCCCCACA AGTGCAGGGA CTGCGACATG 
GCGTTTGTCA CCAGTGGAGA ACTCGTCCGG CACAGGCGTT ACAAACACAC TTATGAGAAG 
CCCTTCAAGT GCTCCCTGTG CAAGTACGCC AGCGTCGAGG CAAGCAAGAT GAAGCGTCAC 
ATCCGCTCAC ACACGGGTGA GCGTCCCTTC CAGTGTTGCC AGTGTGCTTA CGCCAGCAGG 
GACTCCTACA AGCTGAAGCG CCACATGAGG ACACACTCAG GTGAGAAGCC GTATGAATGT 
CCCACCTGTC ACGTCCGGTT CACCCAGAGC GGGACCATGA AAATCCATAT AGCACAGAAG 
CACGGAGAGA ATGTGCCCAA ATACGAGTGT CCCCACTGTG CCACCATCAT CGCGAGGAAG 
AGCGACCTGC GTGTCCATCT GCGTAACCTG CACAGCCAGA GCCCGGAGGA GATGAAGTGC 
CGATACTGTC CCGCTGGCTT CCATGAGCGC TATGCCCTCA TTCAGCACCA GAGGACCCAC 
AAGAACGAGA AGAAGTTCAA GTGCAAGCAG TGCGATTACG CGTGCAAGCA GGAGCGATGC 
TTGAAGGCGC ACATGCGCAT GCACACAGGA GAGAAGCCCT TCTCCTGCCT GGCCTGCAAC 
AAGCACTTCC GACAGAAGCA GCTACTGACC GTGCACCTGA GGAAGTACCA TGACCCGAAC 
TTCGTCCCCA ATCTGCACCT GTGCCTCAAG TGTGATAAAC GTTTCTCCCG CTGGAGTAAC 
CTGCAGAGAC ACAGAAAGAA GTGTGACCCG GAGCATGAGA CGTTAGCCCC CAACAAGGAC 
AGGAGACCAG TGACAAGGAC ACAGGCCTCG GAGGGAGAAG CAGGACACAA GGAAGATGTT 
GCCACCCAAG AGAGCGCCAC CTCAGGGGAG CCTCAGTGCC CTGGGGAGCA GGCTCTGGGC 
CACCAAGGAG AAGCAGCGGG GAGCCAGAGC CCAGACCACG GCCTTACCTG CGAGATAATC 
TTTAACATGA TGGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342114.1
CDS	249..2189
Translation	MAAAEVPVPSGYFTQIKEQKLKPGDLEEEKEEDGVQRVEAQEGVVKEVEAENSCLLLEARAPVESDRRILTLQTVHLESQDVHLQGLGWLSVPHSEELSGTVPEAEGILQLPSVLWLDPEPQLSLQHCVTVSIPEELYPPEELQRIHFHLLRENVLMAEENPELTPDLDESTALKKPEEDEKDQLPPQGETDKREERLLLLEMKPKEGKDDEIVLTISHLSLEEQQDPPAANQTSVPGAKAAKPKRRRQTKGKPQSFQCDTCPFTSSKLSTFNRHIKIHSNERPHLCHLCLKAFRTVTLLRNHVNTHTGTRPHKCRDCDMAFVTSGELVRHRRYKHTYEKPFKCSLCKYASVEASKMKRHIRSHTGERPFQCCQCAYASRDSYKLKRHMRTHSGEKPYECPTCHVRFTQSGTMKIHIAQKHGENVPKYECPHCATIIARKSDLRVHLRNLHSQSPEEMKCRYCPAGFHERYALIQHQRTHKNEKKFKCKQCDYACKQERCLKAHMRMHTGEKPFSCLACNKHFRQKQLLTVHLRKYHDPNFVPNLHLCLKCDKRFSRWSNLQRHRKKCDPEHETLAPNKDRRPVTRTQASEGEAGHKEDVATQESATSGEPQCPGEQALGHQGEAAGSQSPDHGLTCEIIFNMMDK

Target ORF information:

RefSeq Version	NM_001355185.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355185.1

ORF Insert Sequence:

ATGGCTGCCG CTGAGGTCCC TGTCCCTTCT GGGTACTTCA CCCAGATCAA AGAGCAGAAG 
TTGAAGCCTG GAGACCTAGA GGAGGAGAAA GAGGAGGACG GGGTACAAAG AGTGGAAGCC 
CAGGAGGGAG TTGTCAAGGA GGTGGAGGCC GAGAACAGTT GCCTGCTTCT GGAGGCCAGG 
GCCCCGGTGG AGAGCGACAG GCGGATCCTG ACCCTGCAAA CGGTGCACCT GGAGTCCCAG 
GATGTGCACC TACAGGGGCT GGGATGGCTG AGCGTGCCAC ACTCTGAGGA GCTTTCAGGG 
ACGGTACCAG AGGCGGAAGG CATACTGCAG TTGCCATCCG TGCTGTGGCT CGACCCAGAG 
CCCCAGCTCA GCCTTCAGCA TTGCGTGACG GTCAGCATCC CGGAAGAGCT GTACCCACCA 
GAGGAGCTGC AGCGGATACA TTTTCACCTG CTGAGAGAGA ATGTGCTAAT GGCCGAGGAG 
AACCCAGAGT TAACACCAGA CTTGGACGAA AGCACAGCCC TGAAAAAGCC CGAAGAAGAT 
GAAAAGGACC AGCTCCCGCC CCAGGGAGAG ACAGACAAGA GAGAAGAGAG GTTGCTCCTT 
CTGGAAATGA AACCAAAAGA GGGAAAAGAC GACGAAATTG TCCTGACCAT TTCCCATCTA 
AGCCTCGAAG AACAGCAAGA TCCACCAGCG GCCAATCAGA CAAGTGTGCC GGGAGCCAAA 
GCCGCAAAAC CAAAACGGCG GAGGCAGACC AAGGGAAAGC CTCAGAGCTT TCAGTGTGAC 
ACCTGCCCGT TCACTTCCTC CAAGCTCTCA ACTTTCAATC GTCACATCAA AATTCACAGC 
AATGAGAGGC CACACCTGTG TCACCTGTGC CTGAAGGCCT TCCGGACTGT CACTCTTCTT 
AGGAACCATG TGAACACCCA CACAGGAACC AGGCCCCACA AGTGCAGGGA CTGCGACATG 
GCGTTTGTCA CCAGTGGAGA ACTCGTCCGG CACAGGCGTT ACAAACACAC TTATGAGAAG 
CCCTTCAAGT GCTCCCTGTG CAAGTACGCC AGCGTCGAGG CAAGCAAGAT GAAGCGTCAC 
ATCCGCTCAC ACACGGGTGA GCGTCCCTTC CAGTGTTGCC AGTGTGCTTA CGCCAGCAGG 
GACTCCTACA AGCTGAAGCG CCACATGAGG ACACACTCAG GTGAGAAGCC GTATGAATGT 
CCCACCTGTC ACGTCCGGTT CACCCAGAGC GGGACCATGA AAATCCATAT AGCACAGAAG 
CACGGAGAGA ATGTGCCCAA ATACGAGTGT CCCCACTGTG CCACCATCAT CGCGAGGAAG 
AGCGACCTGC GTGTCCATCT GCGTAACCTG CACAGCCAGA GCCCGGAGGA GATGAAGTGC 
CGATACTGTC CCGCTGGCTT CCATGAGCGC TATGCCCTCA TTCAGCACCA GAGGACCCAC 
AAGAACGAGA AGAAGTTCAA GTGCAAGCAG TGCGATTACG CGTGCAAGCA GGAGCGATGC 
TTGAAGGCGC ACATGCGCAT GCACACAGGA GAGAAGCCCT TCTCCTGCCT GGCCTGCAAC 
AAGCACTTCC GACAGAAGCA GCTACTGACC GTGCACCTGA GGAAGTACCA TGACCCGAAC 
TTCGTCCCCA ATCTGCACCT GTGCCTCAAG TGTGATAAAC GTTTCTCCCG CTGGAGTAAC 
CTGCAGAGAC ACAGAAAGAA GTGTGACCCG GAGCATGAGA CGTTAGCCCC CAACAAGGAC 
AGGAGACCAG TGACAAGGAC ACAGGCCTCG GAGGGAGAAG CAGGACACAA GGAAGATGTT 
GCCACCCAAG AGAGCGCCAC CTCAGGGGAG CCTCAGTGCC CTGGGGAGCA GGCTCTGGGC 
CACCAAGGAG AAGCAGCGGG GAGCCAGAGC CCAGACCACG GCCTTACCTG CGAGATAATC 
TTTAACATGA TGGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctcfl ( XM_017319168.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174657.1 Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu30774
Clone ID Related Accession (Same CDS sequence)	XM_017319168.1 , NM_001355185.1
Accession Version	XM_017319168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcriptional repressor CTCFL isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCG CTGAGGTCCC TGTCCCTTCT GGGTACTTCA CCCAGATCAA AGAGCAGAAG 
TTGAAGCCTG GAGACCTAGA GGAGGAGAAA GAGGAGGACG GGGTACAAAG AGTGGAAGCC 
CAGGAGGGAG TTGTCAAGGA GGTGGAGGCC GAGAACAGTT GCCTGCTTCT GGAGGCCAGG 
GCCCCGGTGG AGAGCGACAG GCGGATCCTG ACCCTGCAAA CGGTGCACCT GGAGTCCCAG 
GATGTGCACC TACAGGGGCT GGGATGGCTG AGCGTGCCAC ACTCTGAGGA GCTTTCAGGG 
ACGGTACCAG AGGCGGAAGG CATACTGCAG TTGCCATCCG TGCTGTGGCT CGACCCAGAG 
CCCCAGCTCA GCCTTCAGCA TTGCGTGACG GTCAGCATCC CGGAAGAGCT GTACCCACCA 
GAGGAGCTGC AGCGGATACA TTTTCACCTG CTGAGAGAGA ATGTGCTAAT GGCCGAGGAG 
AACCCAGAGT TAACACCAGA CTTGGACGAA AGCACAGCCC TGAAAAAGCC CGAAGAAGAT 
GAAAAGGACC AGCTCCCGCC CCAGGGAGAG ACAGACAAGA GAGAAGAGAG GTTGCTCCTT 
CTGGAAATGA AACCAAAAGA GGGAAAAGAC GACGAAATTG TCCTGACCAT TTCCCATCTA 
AGCCTCGAAG AACAGCAAGA TCCACCAGCG GCCAATCAGA CAAGTGTGCC GGGAGCCAAA 
GCCGCAAAAC CAAAACGGCG GAGGCAGACC AAGGGAAAGC CTCAGAGCTT TCAGTGTGAC 
ACCTGCCCGT TCACTTCCTC CAAGCTCTCA ACTTTCAATC GTCACATCAA AATTCACAGC 
AATGAGAGGC CACACCTGTG TCACCTGTGC CTGAAGGCCT TCCGGACTGT CACTCTTCTT 
AGGAACCATG TGAACACCCA CACAGGAACC AGGCCCCACA AGTGCAGGGA CTGCGACATG 
GCGTTTGTCA CCAGTGGAGA ACTCGTCCGG CACAGGCGTT ACAAACACAC TTATGAGAAG 
CCCTTCAAGT GCTCCCTGTG CAAGTACGCC AGCGTCGAGG CAAGCAAGAT GAAGCGTCAC 
ATCCGCTCAC ACACGGGTGA GCGTCCCTTC CAGTGTTGCC AGTGTGCTTA CGCCAGCAGG 
GACTCCTACA AGCTGAAGCG CCACATGAGG ACACACTCAG GTGAGAAGCC GTATGAATGT 
CCCACCTGTC ACGTCCGGTT CACCCAGAGC GGGACCATGA AAATCCATAT AGCACAGAAG 
CACGGAGAGA ATGTGCCCAA ATACGAGTGT CCCCACTGTG CCACCATCAT CGCGAGGAAG 
AGCGACCTGC GTGTCCATCT GCGTAACCTG CACAGCCAGA GCCCGGAGGA GATGAAGTGC 
CGATACTGTC CCGCTGGCTT CCATGAGCGC TATGCCCTCA TTCAGCACCA GAGGACCCAC 
AAGAACGAGA AGAAGTTCAA GTGCAAGCAG TGCGATTACG CGTGCAAGCA GGAGCGATGC 
TTGAAGGCGC ACATGCGCAT GCACACAGGA GAGAAGCCCT TCTCCTGCCT GGCCTGCAAC 
AAGCACTTCC GACAGAAGCA GCTACTGACC GTGCACCTGA GGAAGTACCA TGACCCGAAC 
TTCGTCCCCA ATCTGCACCT GTGCCTCAAG TGTGATAAAC GTTTCTCCCG CTGGAGTAAC 
CTGCAGAGAC ACAGAAAGAA GTGTGACCCG GAGCATGAGA CGTTAGCCCC CAACAAGGAC 
AGGAGACCAG TGACAAGGAC ACAGGCCTCG GAGGGAGAAG CAGGACACAA GGAAGATGTT 
GCCACCCAAG AGAGCGCCAC CTCAGGGGAG CCTCAGTGCC CTGGGGAGCA GGCTCTGGGC 
CACCAAGGAG AAGCAGCGGG GAGCCAGAGC CCAGACCACG GCCTTACCTG CGAGATAATC 
TTTAACATGA TGGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174657.1
CDS	15..1955
Translation	MAAAEVPVPSGYFTQIKEQKLKPGDLEEEKEEDGVQRVEAQEGVVKEVEAENSCLLLEARAPVESDRRILTLQTVHLESQDVHLQGLGWLSVPHSEELSGTVPEAEGILQLPSVLWLDPEPQLSLQHCVTVSIPEELYPPEELQRIHFHLLRENVLMAEENPELTPDLDESTALKKPEEDEKDQLPPQGETDKREERLLLLEMKPKEGKDDEIVLTISHLSLEEQQDPPAANQTSVPGAKAAKPKRRRQTKGKPQSFQCDTCPFTSSKLSTFNRHIKIHSNERPHLCHLCLKAFRTVTLLRNHVNTHTGTRPHKCRDCDMAFVTSGELVRHRRYKHTYEKPFKCSLCKYASVEASKMKRHIRSHTGERPFQCCQCAYASRDSYKLKRHMRTHSGEKPYECPTCHVRFTQSGTMKIHIAQKHGENVPKYECPHCATIIARKSDLRVHLRNLHSQSPEEMKCRYCPAGFHERYALIQHQRTHKNEKKFKCKQCDYACKQERCLKAHMRMHTGEKPFSCLACNKHFRQKQLLTVHLRKYHDPNFVPNLHLCLKCDKRFSRWSNLQRHRKKCDPEHETLAPNKDRRPVTRTQASEGEAGHKEDVATQESATSGEPQCPGEQALGHQGEAAGSQSPDHGLTCEIIFNMMDK

Target ORF information:

RefSeq Version	XM_017319168.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319168.1

ORF Insert Sequence:

ATGGCTGCCG CTGAGGTCCC TGTCCCTTCT GGGTACTTCA CCCAGATCAA AGAGCAGAAG 
TTGAAGCCTG GAGACCTAGA GGAGGAGAAA GAGGAGGACG GGGTACAAAG AGTGGAAGCC 
CAGGAGGGAG TTGTCAAGGA GGTGGAGGCC GAGAACAGTT GCCTGCTTCT GGAGGCCAGG 
GCCCCGGTGG AGAGCGACAG GCGGATCCTG ACCCTGCAAA CGGTGCACCT GGAGTCCCAG 
GATGTGCACC TACAGGGGCT GGGATGGCTG AGCGTGCCAC ACTCTGAGGA GCTTTCAGGG 
ACGGTACCAG AGGCGGAAGG CATACTGCAG TTGCCATCCG TGCTGTGGCT CGACCCAGAG 
CCCCAGCTCA GCCTTCAGCA TTGCGTGACG GTCAGCATCC CGGAAGAGCT GTACCCACCA 
GAGGAGCTGC AGCGGATACA TTTTCACCTG CTGAGAGAGA ATGTGCTAAT GGCCGAGGAG 
AACCCAGAGT TAACACCAGA CTTGGACGAA AGCACAGCCC TGAAAAAGCC CGAAGAAGAT 
GAAAAGGACC AGCTCCCGCC CCAGGGAGAG ACAGACAAGA GAGAAGAGAG GTTGCTCCTT 
CTGGAAATGA AACCAAAAGA GGGAAAAGAC GACGAAATTG TCCTGACCAT TTCCCATCTA 
AGCCTCGAAG AACAGCAAGA TCCACCAGCG GCCAATCAGA CAAGTGTGCC GGGAGCCAAA 
GCCGCAAAAC CAAAACGGCG GAGGCAGACC AAGGGAAAGC CTCAGAGCTT TCAGTGTGAC 
ACCTGCCCGT TCACTTCCTC CAAGCTCTCA ACTTTCAATC GTCACATCAA AATTCACAGC 
AATGAGAGGC CACACCTGTG TCACCTGTGC CTGAAGGCCT TCCGGACTGT CACTCTTCTT 
AGGAACCATG TGAACACCCA CACAGGAACC AGGCCCCACA AGTGCAGGGA CTGCGACATG 
GCGTTTGTCA CCAGTGGAGA ACTCGTCCGG CACAGGCGTT ACAAACACAC TTATGAGAAG 
CCCTTCAAGT GCTCCCTGTG CAAGTACGCC AGCGTCGAGG CAAGCAAGAT GAAGCGTCAC 
ATCCGCTCAC ACACGGGTGA GCGTCCCTTC CAGTGTTGCC AGTGTGCTTA CGCCAGCAGG 
GACTCCTACA AGCTGAAGCG CCACATGAGG ACACACTCAG GTGAGAAGCC GTATGAATGT 
CCCACCTGTC ACGTCCGGTT CACCCAGAGC GGGACCATGA AAATCCATAT AGCACAGAAG 
CACGGAGAGA ATGTGCCCAA ATACGAGTGT CCCCACTGTG CCACCATCAT CGCGAGGAAG 
AGCGACCTGC GTGTCCATCT GCGTAACCTG CACAGCCAGA GCCCGGAGGA GATGAAGTGC 
CGATACTGTC CCGCTGGCTT CCATGAGCGC TATGCCCTCA TTCAGCACCA GAGGACCCAC 
AAGAACGAGA AGAAGTTCAA GTGCAAGCAG TGCGATTACG CGTGCAAGCA GGAGCGATGC 
TTGAAGGCGC ACATGCGCAT GCACACAGGA GAGAAGCCCT TCTCCTGCCT GGCCTGCAAC 
AAGCACTTCC GACAGAAGCA GCTACTGACC GTGCACCTGA GGAAGTACCA TGACCCGAAC 
TTCGTCCCCA ATCTGCACCT GTGCCTCAAG TGTGATAAAC GTTTCTCCCG CTGGAGTAAC 
CTGCAGAGAC ACAGAAAGAA GTGTGACCCG GAGCATGAGA CGTTAGCCCC CAACAAGGAC 
AGGAGACCAG TGACAAGGAC ACAGGCCTCG GAGGGAGAAG CAGGACACAA GGAAGATGTT 
GCCACCCAAG AGAGCGCCAC CTCAGGGGAG CCTCAGTGCC CTGGGGAGCA GGCTCTGGGC 
CACCAAGGAG AAGCAGCGGG GAGCCAGAGC CCAGACCACG GCCTTACCTG CGAGATAATC 
TTTAACATGA TGGATAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctcfl ( XM_017319167.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174656.1 Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80366
Clone ID Related Accession (Same CDS sequence)	XM_017319167.1 , XM_017319167.2
Accession Version	XM_017319167.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcriptional repressor CTCFL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTG TCACCAGTGG AGAACTCGTC CGGCACAGGC GTTACAAACA CACTTATGAG 
AAGCCCTTCA AGTGCTCCCT GTGCAAGTAC GCCAGCGTCG AGGCAAGCAA GATGAAGCGT 
CACATCCGCT CACACACGGG TGAGCGTCCC TTCCAGTGTT GCCAGTGTGC TTACGCCAGC 
AGGGACTCCT ACAAGCTGAA GCGCCACATG AGGACACACT CAGGTGAGAA GCCGTATGAA 
TGTCCCACCT GTCACGTCCG GTTCACCCAG AGCGGGACCA TGAAAATCCA TATAGCACAG 
AAGCACGGAG AGAATGTGCC CAAATACGAG TGTCCCCACT GTGCCACCAT CATCGCGAGG 
AAGAGCGACC TGCGTGTCCA TCTGCGTAAC CTGCACAGCC AGAGCCCGGA GGAGATGAAG 
TGCCGATACT GTCCCGCTGG CTTCCATGAG CGCTATGCCC TCATTCAGCA CCAGAGGACC 
CACAAGAACG AGAAGAAGTT CAAGTGCAAG CAGTGCGATT ACGCGTGCAA GCAGGAGCGA 
TGCTTGAAGG CGCACATGCG CATGCACACA GGAGAGAAGC CCTTCTCCTG CCTGGCCTGC 
AACAAGCACT TCCGACAGAA GCAGCTACTG ACCGTGCACC TGAGGAAGTA CCATGACCCG 
AACTTCGTCC CCAATCTGCA CCTGTGCCTC AAGTGTGATA AACGTTTCTC CCGCTGGAGT 
AACCTGCAGA GACACAGAAA GAAGTGTGAC CCGGAGCATG AGACGTTAGC CCCCAACAAG 
GACAGGAGAC CAGTGACAAG GACACAGGCC TCGGAGGGAG AAGCAGGACA CAAGGAAGAT 
GTTGCCACCC AAGAGAGCGC CACCTCAGGG GAGCCTCAGT GCCCTGGGGA GCAGGCTCTG 
GGCCACCAAG GAGAAGCAGC GGGGAGCCAG AGCCCAGACC ACGGCCTTAC CTGCGAGATA 
ATCTTTAACA TGATGGATAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174656.1
CDS	81..1064
Translation	MAFVTSGELVRHRRYKHTYEKPFKCSLCKYASVEASKMKRHIRSHTGERPFQCCQCAYASRDSYKLKRHMRTHSGEKPYECPTCHVRFTQSGTMKIHIAQKHGENVPKYECPHCATIIARKSDLRVHLRNLHSQSPEEMKCRYCPAGFHERYALIQHQRTHKNEKKFKCKQCDYACKQERCLKAHMRMHTGEKPFSCLACNKHFRQKQLLTVHLRKYHDPNFVPNLHLCLKCDKRFSRWSNLQRHRKKCDPEHETLAPNKDRRPVTRTQASEGEAGHKEDVATQESATSGEPQCPGEQALGHQGEAAGSQSPDHGLTCEIIFNMMDK

Target ORF information:

RefSeq Version	XM_017319167.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319167.1

ORF Insert Sequence:

ATGGCGTTTG TCACCAGTGG AGAACTCGTC CGGCACAGGC GTTACAAACA CACTTATGAG 
AAGCCCTTCA AGTGCTCCCT GTGCAAGTAC GCCAGCGTCG AGGCAAGCAA GATGAAGCGT 
CACATCCGCT CACACACGGG TGAGCGTCCC TTCCAGTGTT GCCAGTGTGC TTACGCCAGC 
AGGGACTCCT ACAAGCTGAA GCGCCACATG AGGACACACT CAGGTGAGAA GCCGTATGAA 
TGTCCCACCT GTCACGTCCG GTTCACCCAG AGCGGGACCA TGAAAATCCA TATAGCACAG 
AAGCACGGAG AGAATGTGCC CAAATACGAG TGTCCCCACT GTGCCACCAT CATCGCGAGG 
AAGAGCGACC TGCGTGTCCA TCTGCGTAAC CTGCACAGCC AGAGCCCGGA GGAGATGAAG 
TGCCGATACT GTCCCGCTGG CTTCCATGAG CGCTATGCCC TCATTCAGCA CCAGAGGACC 
CACAAGAACG AGAAGAAGTT CAAGTGCAAG CAGTGCGATT ACGCGTGCAA GCAGGAGCGA 
TGCTTGAAGG CGCACATGCG CATGCACACA GGAGAGAAGC CCTTCTCCTG CCTGGCCTGC 
AACAAGCACT TCCGACAGAA GCAGCTACTG ACCGTGCACC TGAGGAAGTA CCATGACCCG 
AACTTCGTCC CCAATCTGCA CCTGTGCCTC AAGTGTGATA AACGTTTCTC CCGCTGGAGT 
AACCTGCAGA GACACAGAAA GAAGTGTGAC CCGGAGCATG AGACGTTAGC CCCCAACAAG 
GACAGGAGAC CAGTGACAAG GACACAGGCC TCGGAGGGAG AAGCAGGACA CAAGGAAGAT 
GTTGCCACCC AAGAGAGCGC CACCTCAGGG GAGCCTCAGT GCCCTGGGGA GCAGGCTCTG 
GGCCACCAAG GAGAAGCAGC GGGGAGCCAG AGCCCAGACC ACGGCCTTAC CTGCGAGATA 
ATCTTTAACA TGATGGATAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctcfl ( XM_017319167.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174656.1 Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80366
Clone ID Related Accession (Same CDS sequence)	XM_017319167.1 , XM_017319167.2
Accession Version	XM_017319167.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transcriptional repressor CTCFL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTG TCACCAGTGG AGAACTCGTC CGGCACAGGC GTTACAAACA CACTTATGAG 
AAGCCCTTCA AGTGCTCCCT GTGCAAGTAC GCCAGCGTCG AGGCAAGCAA GATGAAGCGT 
CACATCCGCT CACACACGGG TGAGCGTCCC TTCCAGTGTT GCCAGTGTGC TTACGCCAGC 
AGGGACTCCT ACAAGCTGAA GCGCCACATG AGGACACACT CAGGTGAGAA GCCGTATGAA 
TGTCCCACCT GTCACGTCCG GTTCACCCAG AGCGGGACCA TGAAAATCCA TATAGCACAG 
AAGCACGGAG AGAATGTGCC CAAATACGAG TGTCCCCACT GTGCCACCAT CATCGCGAGG 
AAGAGCGACC TGCGTGTCCA TCTGCGTAAC CTGCACAGCC AGAGCCCGGA GGAGATGAAG 
TGCCGATACT GTCCCGCTGG CTTCCATGAG CGCTATGCCC TCATTCAGCA CCAGAGGACC 
CACAAGAACG AGAAGAAGTT CAAGTGCAAG CAGTGCGATT ACGCGTGCAA GCAGGAGCGA 
TGCTTGAAGG CGCACATGCG CATGCACACA GGAGAGAAGC CCTTCTCCTG CCTGGCCTGC 
AACAAGCACT TCCGACAGAA GCAGCTACTG ACCGTGCACC TGAGGAAGTA CCATGACCCG 
AACTTCGTCC CCAATCTGCA CCTGTGCCTC AAGTGTGATA AACGTTTCTC CCGCTGGAGT 
AACCTGCAGA GACACAGAAA GAAGTGTGAC CCGGAGCATG AGACGTTAGC CCCCAACAAG 
GACAGGAGAC CAGTGACAAG GACACAGGCC TCGGAGGGAG AAGCAGGACA CAAGGAAGAT 
GTTGCCACCC AAGAGAGCGC CACCTCAGGG GAGCCTCAGT GCCCTGGGGA GCAGGCTCTG 
GGCCACCAAG GAGAAGCAGC GGGGAGCCAG AGCCCAGACC ACGGCCTTAC CTGCGAGATA 
ATCTTTAACA TGATGGATAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174656.1
CDS	81..1064
Translation	MAFVTSGELVRHRRYKHTYEKPFKCSLCKYASVEASKMKRHIRSHTGERPFQCCQCAYASRDSYKLKRHMRTHSGEKPYECPTCHVRFTQSGTMKIHIAQKHGENVPKYECPHCATIIARKSDLRVHLRNLHSQSPEEMKCRYCPAGFHERYALIQHQRTHKNEKKFKCKQCDYACKQERCLKAHMRMHTGEKPFSCLACNKHFRQKQLLTVHLRKYHDPNFVPNLHLCLKCDKRFSRWSNLQRHRKKCDPEHETLAPNKDRRPVTRTQASEGEAGHKEDVATQESATSGEPQCPGEQALGHQGEAAGSQSPDHGLTCEIIFNMMDK

Target ORF information:

RefSeq Version	XM_017319167.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CCCTC-binding factor (zinc finger protein)-like (Ctcfl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319167.2

ORF Insert Sequence:

ATGGCGTTTG TCACCAGTGG AGAACTCGTC CGGCACAGGC GTTACAAACA CACTTATGAG 
AAGCCCTTCA AGTGCTCCCT GTGCAAGTAC GCCAGCGTCG AGGCAAGCAA GATGAAGCGT 
CACATCCGCT CACACACGGG TGAGCGTCCC TTCCAGTGTT GCCAGTGTGC TTACGCCAGC 
AGGGACTCCT ACAAGCTGAA GCGCCACATG AGGACACACT CAGGTGAGAA GCCGTATGAA 
TGTCCCACCT GTCACGTCCG GTTCACCCAG AGCGGGACCA TGAAAATCCA TATAGCACAG 
AAGCACGGAG AGAATGTGCC CAAATACGAG TGTCCCCACT GTGCCACCAT CATCGCGAGG 
AAGAGCGACC TGCGTGTCCA TCTGCGTAAC CTGCACAGCC AGAGCCCGGA GGAGATGAAG 
TGCCGATACT GTCCCGCTGG CTTCCATGAG CGCTATGCCC TCATTCAGCA CCAGAGGACC 
CACAAGAACG AGAAGAAGTT CAAGTGCAAG CAGTGCGATT ACGCGTGCAA GCAGGAGCGA 
TGCTTGAAGG CGCACATGCG CATGCACACA GGAGAGAAGC CCTTCTCCTG CCTGGCCTGC 
AACAAGCACT TCCGACAGAA GCAGCTACTG ACCGTGCACC TGAGGAAGTA CCATGACCCG 
AACTTCGTCC CCAATCTGCA CCTGTGCCTC AAGTGTGATA AACGTTTCTC CCGCTGGAGT 
AACCTGCAGA GACACAGAAA GAAGTGTGAC CCGGAGCATG AGACGTTAGC CCCCAACAAG 
GACAGGAGAC CAGTGACAAG GACACAGGCC TCGGAGGGAG AAGCAGGACA CAAGGAAGAT 
GTTGCCACCC AAGAGAGCGC CACCTCAGGG GAGCCTCAGT GCCCTGGGGA GCAGGCTCTG 
GGCCACCAAG GAGAAGCAGC GGGGAGCCAG AGCCCAGACC ACGGCCTTAC CTGCGAGATA 
ATCTTTAACA TGATGGATAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Testis-specific transcriptional regulators selectively occupy BORIS-bound CTCF target regions in mouse male germ cells.
Scientific reports741279(2017 Feb)
Rivero-Hinojosa S,Kang S,Lobanenkov VV,Zentner GE

Expression of the CTCFL Gene during Mouse Embryogenesis Causes Growth Retardation, Postnatal Lethality, and Dysregulation of the Transforming Growth Factor ? Pathway.
Molecular and cellular biology35(19)3436-45(2015 Oct)
Sati L,Zeiss C,Yekkala K,Demir R,McGrath J

The male germ cell gene regulator CTCFL is functionally different from CTCF and binds CTCF-like consensus sites in a nucleosome composition-dependent manner.
Epigenetics & chromatin5(1)8(2012 Jun)
Sleutels F,Soochit W,Bartkuhn M,Heath H,Dienstbach S,Bergmaier P,Franke V,Rosa-Garrido M,van de Nobelen S,Caesar L,van der Reijden M,Bryne JC,van Ijcken W,Grootegoed JA,Delgado MD,Lenhard B,Renkawitz R,Grosveld F,Galjart N

Integrating role of T antigen, Rb2/p130, CTCF and BORIS in mediating non-canonical endoplasmic reticulum-dependent death pathways triggered by chronic ER stress in mouse medulloblastoma.
Cell cycle (Georgetown, Tex.)11(9)1841-50(2012 May)
Macaluso M,Caracciolo V,Rizzo V,Sun A,Montanari M,Russo G,Bellipanni G,Khalili K,Giordano A

A cell cycle role for the epigenetic factor CTCF-L/BORIS.
PloS one7(6)e39371(2012)
Rosa-Garrido M,Ceballos L,Alonso-Lecue P,Abraira C,Delgado MD,Gandarillas A

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Ctcfl cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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