Magohb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Magohb
Entrez Gene ID	66441
Full Name	mago homolog B, exon junction complex core component
Synonyms	2010012C16Rik,Mago,Magoh,Magoh-rs1
General protein information	Preferred Names mago homolog B, exon junction complex core component Names protein mago nashi homolog 2 mago nashi protein mago-nashi homolog B mago-nashi homolog, proliferation-associated, related sequence 1 protein mago nashi homolog 1-related
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F3\|6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001327860.1	NP_001314789.1	protein mago nashi homolog 2 isoform 2
NM_025564.2	NP_079840.2	protein mago nashi homolog 2 isoform 1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010755	XP_318556.2
Caenorhabditis elegans (roundworm)	mag-1	NP_493025.1
Neurospora crassa	NCU04405	XP_957482.1
Arabidopsis thaliana (thale cress)	MAGO	NP_171716.1
Oryza sativa (rice)	Os12g0287200	NP_001066589.1
Oryza sativa (rice)	Os08g0107900	NP_001060802.2
Rattus norvegicus (Norway rat)	Magohb	NP_001178034.1
Xenopus tropicalis (tropical clawed frog)	magoh	XP_002931471.1
Homo sapiens (human)	MAGOHB	NP_060518.1
Bos taurus (cattle)	MAGOHB	NP_001069720.1
Mus musculus (house mouse)	Magohb	NP_079840.2
Danio rerio (zebrafish)	magoh	NP_001017700.1
Schizosaccharomyces pombe (fission yeast)	mnh1	NP_596666.1
Pan troglodytes (chimpanzee)	MAGOHB	XP_520743.2
	MAGOHB	XP_001115401.2
Drosophila melanogaster (fruit fly)	mago	NP_476636.1
Magnaporthe oryzae (rice blast fungus)	MGG_06859	XP_003709581.1

Gene Ontology
Bibliography

Related articles in PubMed

Slow co-evolution of the MAGO and Y14 protein families is required for the maintenance of their obligate heterodimerization mode.
Gong P, Zhao M, He C
PloS one9(1)e84842(2014)

Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay.
Singh KK, Wachsmuth L, Kulozik AE, Gehring NH
RNA biology10(8)1291-8(2013 Aug)

The histone methyltransferase KMT2B is required for RNA polymerase II association and protection from DNA methylation at the MagohB CpG island promoter.
Ladopoulos V, Hofemeister H, Hoogenkamp M, Riggs AD, Stewart AF, Bonifer C
Molecular and cellular biology33(7)1383-93(2013 Apr)

Sequence-ready BAC contig, physical, and transcriptional map of a 2-Mb region overlapping the mouse chromosome 6 host-resistance locus Cmv1.
Depatie C, Lee SH, Stafford A, Avner P, Belouchi A, Gros P, Vidal SM
Genomics66(2)161-74(2000 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings show 2 genes MAGOH and MAGOHB are expressed in mammals; in macrophages, expression of MAGOHB but not MAGOH mRNA increases after LPS stimulation; both MAGOH proteins interact with other exon junction complex (EJC) components, incorporate into mRNA-bound EJCs and activate nonsense-mediated decay
Title: Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay.

Reexpression of KMT2B was sufficient to reinstate an active MagohB promoter.
Title: The histone methyltransferase KMT2B is required for RNA polymerase II association and protection from DNA methylation at the MagohB CpG island promoter.

The following Magohb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Magohb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu37506	NM_001327860.1 Latest version!	Mus musculus mago homolog B, exon junction complex core component (Magohb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu19091	NM_025564.2 Latest version!	Mus musculus mago homolog B, exon junction complex core component (Magohb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Magohb ( NM_001327860.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001314789.1 Mus musculus mago homolog B, exon junction complex core component (Magohb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu37506
Clone ID Related Accession (Same CDS sequence)	NM_001327860.1
Accession Version	NM_001327860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	protein mago nashi homolog 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008200.1 and BE649735.1. On May 27, 2016 this sequence version replaced XM_006506474.1. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, resulting in the use of a downstream in-frame translational start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BE649735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGATCAGAA AAGAGGCCTA TGTCCACAAG AGTGTAATGG AAGAACTGAA GAGAATTATT 
GACGACAGTG AAATTACAAA AGAGGACGAC GCTCTGTGGC CACCCCCTGA CAGGGTTGGG 
CGGCAGGAAC TTGAAATCGT AATTGGGGAT GAACACATTT CTTTTACCAC GTCAAAAATA 
GGTTCTCTTA TTGATGTAAA TCAGTCAAAG GATCCTGAAG GCCTTCGTGT ATTTTACTAT 
TTGGTACAAG ACTTGAAATG CTTAGTGTTC AGCCTCATTG GTCTACACTT CAAGATTAAG 
CCAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314789.1
CDS	418..726
Translation	MIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLHFKIKPI

Target ORF information:

RefSeq Version	NM_001327860.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mago homolog B, exon junction complex core component (Magohb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327860.1

ORF Insert Sequence:

ATGATCAGAA AAGAGGCCTA TGTCCACAAG AGTGTAATGG AAGAACTGAA GAGAATTATT 
GACGACAGTG AAATTACAAA AGAGGACGAC GCTCTGTGGC CACCCCCTGA CAGGGTTGGG 
CGGCAGGAAC TTGAAATCGT AATTGGGGAT GAACACATTT CTTTTACCAC GTCAAAAATA 
GGTTCTCTTA TTGATGTAAA TCAGTCAAAG GATCCTGAAG GCCTTCGTGT ATTTTACTAT 
TTGGTACAAG ACTTGAAATG CTTAGTGTTC AGCCTCATTG GTCTACACTT CAAGATTAAG 
CCAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Magohb ( NM_025564.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079840.2 Mus musculus mago homolog B, exon junction complex core component (Magohb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19091
Clone ID Related Accession (Same CDS sequence)	NM_025564.2
Accession Version	NM_025564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	protein mago nashi homolog 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008200.1. On Apr 6, 2007 this sequence version replaced NM_025564.1. Transcript Variant: This variant (1) encodes the longer isoform (1). CCDS Note: The coding region has been updated to extend the N-terminus to one that is also supported by available conservation data. The use of an alternative upstream start codon would result in a protein that is 2 aa longer. ##Evidence-Data-START## Transcript exon combination :: AK008200.1, BY708153.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTATGG GTAGTGATTT CTACCTTCGC TACTACGTCG GGCATAAGGG CAAGTTTGGG 
CACGAGTTTT TGGAGTTCGA GTTTCGGCCG GACGGGAAGC TTAGATATGC CAACAACAGT 
AATTATAAAA ATGACGTGAT GATCAGAAAA GAGGCCTATG TCCACAAGAG TGTAATGGAA 
GAACTGAAGA GAATTATTGA CGACAGTGAA ATTACAAAAG AGGACGACGC TCTGTGGCCA 
CCCCCTGACA GGGTTGGGCG GCAGGAACTT GAAATCGTAA TTGGGGATGA ACACATTTCT 
TTTACCACGT CAAAAATAGG TTCTCTTATT GATGTAAATC AGTCAAAGGA TCCTGAAGGC 
CTTCGTGTAT TTTACTATTT GGTACAAGAC TTGAAATGCT TAGTGTTCAG CCTCATTGGT 
CTACACTTCA AGATTAAGCC AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079840.2
CDS	80..526
Translation	MSMGSDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLHFKIKPI

Target ORF information:

RefSeq Version	NM_025564.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mago homolog B, exon junction complex core component (Magohb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025564.2

ORF Insert Sequence:

ATGTCTATGG GTAGTGATTT CTACCTTCGC TACTACGTCG GGCATAAGGG CAAGTTTGGG 
CACGAGTTTT TGGAGTTCGA GTTTCGGCCG GACGGGAAGC TTAGATATGC CAACAACAGT 
AATTATAAAA ATGACGTGAT GATCAGAAAA GAGGCCTATG TCCACAAGAG TGTAATGGAA 
GAACTGAAGA GAATTATTGA CGACAGTGAA ATTACAAAAG AGGACGACGC TCTGTGGCCA 
CCCCCTGACA GGGTTGGGCG GCAGGAACTT GAAATCGTAA TTGGGGATGA ACACATTTCT 
TTTACCACGT CAAAAATAGG TTCTCTTATT GATGTAAATC AGTCAAAGGA TCCTGAAGGC 
CTTCGTGTAT TTTACTATTT GGTACAAGAC TTGAAATGCT TAGTGTTCAG CCTCATTGGT 
CTACACTTCA AGATTAAGCC AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Slow co-evolution of the MAGO and Y14 protein families is required for the maintenance of their obligate heterodimerization mode.
PloS one9(1)e84842(2014)
Gong P,Zhao M,He C

Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay.
RNA biology10(8)1291-8(2013 Aug)
Singh KK,Wachsmuth L,Kulozik AE,Gehring NH

The histone methyltransferase KMT2B is required for RNA polymerase II association and protection from DNA methylation at the MagohB CpG island promoter.
Molecular and cellular biology33(7)1383-93(2013 Apr)
Ladopoulos V,Hofemeister H,Hoogenkamp M,Riggs AD,Stewart AF,Bonifer C

Sequence-ready BAC contig, physical, and transcriptional map of a 2-Mb region overlapping the mouse chromosome 6 host-resistance locus Cmv1.
Genomics66(2)161-74(2000 Jun)
Depatie C,Lee SH,Stafford A,Avner P,Belouchi A,Gros P,Vidal SM