Tbcb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tbcb
Entrez Gene ID	66411
Full Name	tubulin folding cofactor B
Synonyms	2410007D12Rik,AU041393,CG22,CKAPI,Ckap1
General protein information	Preferred Names tubulin folding cofactor B Names tubulin-folding cofactor B cytoskeleton-associated protein 1 cytoskeleton-associated protein CKAPI tubulin-specific chaperone B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025548.3	NP_079824.2	tubulin-folding cofactor B
NM_025548.4	NP_079824.2	tubulin-folding cofactor B isoform 1
NM_001360648.1	NP_001347577.1	tubulin-folding cofactor B isoform 2
XM_011250672.2	XP_011248974.1	tubulin-folding cofactor B isoform X1
NM_001360649.1	NP_001347578.1	tubulin-folding cofactor B isoform 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TBCB	XP_533685.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000120	XP_311025.2
Saccharomyces cerevisiae (baker's yeast)	ALF1	NP_014251.1
Magnaporthe oryzae (rice blast fungus)	MGG_08570	XP_003716093.1
Homo sapiens (human)	TBCB	NP_001272.2
Mus musculus (house mouse)	Tbcb	NP_079824.2
Kluyveromyces lactis	KLLA0E22859g	XP_002999413.1
Eremothecium gossypii	AGOS_AFR054W	NP_985601.2
Oryza sativa (rice)	Os04g0692100	NP_001054365.1
Xenopus tropicalis (tropical clawed frog)	tbcb	XP_002932660.1
Neurospora crassa	NCU01713	XP_956027.1
Pan troglodytes (chimpanzee)	TBCB	XP_003316381.1
Bos taurus (cattle)	TBCB	NP_001014939.1
Arabidopsis thaliana (thale cress)	AT3G10220	NP_187633.2
	CKAP1	XP_001102227.1
Danio rerio (zebrafish)	tbcb	NP_997940.1
Drosophila melanogaster (fruit fly)	TBCB	NP_611425.1
Caenorhabditis elegans (roundworm)	CELE_F53F4.3	NP_506367.1
Schizosaccharomyces pombe (fission yeast)	alp11	NP_593683.2

Gene Ontology
Bibliography

Related articles in PubMed

Tubulin-binding cofactor B is a direct interaction partner of the dynactin subunit p150(Glued).
Kuh GF, Stockmann M, Meyer-Ohlendorf M, Linta L, Proepper C, Ludolph AC, Bockmann J, Boeckers TM, Liebau S
Cell and tissue research350(1)13-26(2012 Oct)

RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly.
Lo JC, Jamsai D, O'Connor AE, Borg C, Clark BJ, Whisstock JC, Field MC, Adams V, Ishikawa T, Aitken RJ, Whittle B, Goodnow CC, Ormandy CJ, O'Bryan MK
PLoS genetics8(10)e1002969(2012)

Stathmin, a microtubule-destabilizing protein, is dysregulated in spinal muscular atrophy.
Wen HL, Lin YT, Ting CH, Lin-Chao S, Li H, Hsieh-Li HM
Human molecular genetics19(9)1766-78(2010 May)

Tubulin cofactor B plays a role in the neuronal growth cone.
Lopez-Fanarraga M, Carranza G, Bellido J, Kortazar D, Villegas JC, Zabala JC
Journal of neurochemistry100(6)1680-7(2007 Mar)

Role of cofactors B (TBCB) and E (TBCE) in tubulin heterodimer dissociation.
Kortazar D, Fanarraga ML, Carranza G, Bellido J, Villegas JC, Avila J, Zabala JC
Experimental cell research313(3)425-36(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of TBCB leads to the decreased localization of p150 to the microtubule network that might result in a functional modulation of this protein complex.
Title: Tubulin-binding cofactor B is a direct interaction partner of the dynactin subunit p150(Glued).

We show that TBCB overexpression depolymerizes microtubules. We found that this function is based on the ability of TBCB to form a binary complex with TBCE that greatly enhances the efficiency of this cofactor to dissociate tubulin in vivo and in vitro.
Title: Role of cofactors B (TBCB) and E (TBCE) in tubulin heterodimer dissociation.

The following Tbcb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tbcb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69936	NM_001360648.1 Latest version!	Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu69936	XM_011250672.2 Latest version!	Mus musculus tubulin folding cofactor B (Tbcb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu00654	NM_025548.4 Latest version!	Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu84327	NM_001360649.1 Latest version!	Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu00654	NM_025548.3	Mus musculus tubulin folding cofactor B (Tbcb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tbcb ( NM_001360648.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347577.1 Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69936
Clone ID Related Accession (Same CDS sequence)	NM_001360648.1 , XM_011250672.2
Accession Version	NM_001360648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Mus musculus(house mouse)
Product	tubulin-folding cofactor B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149067.3. On Feb 8, 2018 this sequence version replaced XM_011250672.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.539730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTGG AGCTATATGG AGCAGACGAC AAGTTCTACA GCAAGTTGGA TCAGGAGGAT 
GCACTGCTGG GCTCCTATCC TGTGGATGAC GGCTGTCGCA TCCATGTCAT TGACCACAGT 
GGCGTCCGGC TCGGAGAGTA TGAGGATGTG TCCAAAGTGG AGAAATACGA GATCTCACCA 
GAAGCTTACG AACGGAGGCA GAACACAGTT CGATCCTTCA TGAAACGCAG CAAGCTAGGC 
CCGTACAATG AAGAACTCCG GGCCCAGCAA GAGGCAGAGG CTGCCCAGCG TCTCAGTGAG 
GAGAAGGCCC AAGCCAGTGC CATCTCTGTG GGGAGCCGTT GTGAGGTGCG GGCACCCGAC 
CACTCCCTTC GCAGGGGCAC TGTCATGTAT GTAGGACTCA CAGATTTCAA GCCAGGCTAC 
TGGGTTGGTG TCCGATATGA TGAGCCGTTA GGAAAAAACG ATGGCAGTGT GAATGGGAAA 
CGCTACTTTG AATGCCAGGC CAAGTATGGT GCCTTTGTAA AGCCATCAGC TGTCACCGTA 
GGAGATTTCC CAGAGGAGGA CTACGGGTTG GACGAGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347577.1
CDS	74..655
Translation	MELELYGADDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQQEAEAAQRLSEEKAQASAISVGSRCEVRAPDHSLRRGTVMYVGLTDFKPGYWVGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSAVTVGDFPEEDYGLDEM

Target ORF information:

RefSeq Version	NM_001360648.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360648.1

ORF Insert Sequence:

ATGGAACTGG AGCTATATGG AGCAGACGAC AAGTTCTACA GCAAGTTGGA TCAGGAGGAT 
GCACTGCTGG GCTCCTATCC TGTGGATGAC GGCTGTCGCA TCCATGTCAT TGACCACAGT 
GGCGTCCGGC TCGGAGAGTA TGAGGATGTG TCCAAAGTGG AGAAATACGA GATCTCACCA 
GAAGCTTACG AACGGAGGCA GAACACAGTT CGATCCTTCA TGAAACGCAG CAAGCTAGGC 
CCGTACAATG AAGAACTCCG GGCCCAGCAA GAGGCAGAGG CTGCCCAGCG TCTCAGTGAG 
GAGAAGGCCC AAGCCAGTGC CATCTCTGTG GGGAGCCGTT GTGAGGTGCG GGCACCCGAC 
CACTCCCTTC GCAGGGGCAC TGTCATGTAT GTAGGACTCA CAGATTTCAA GCCAGGCTAC 
TGGGTTGGTG TCCGATATGA TGAGCCGTTA GGAAAAAACG ATGGCAGTGT GAATGGGAAA 
CGCTACTTTG AATGCCAGGC CAAGTATGGT GCCTTTGTAA AGCCATCAGC TGTCACCGTA 
GGAGATTTCC CAGAGGAGGA CTACGGGTTG GACGAGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbcb ( XM_011250672.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248974.1 Mus musculus tubulin folding cofactor B (Tbcb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69936
Clone ID Related Accession (Same CDS sequence)	NM_001360648.1 , XM_011250672.2
Accession Version	XM_011250672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tubulin-folding cofactor B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG AGCTATATGG AGCAGACGAC AAGTTCTACA GCAAGTTGGA TCAGGAGGAT 
GCACTGCTGG GCTCCTATCC TGTGGATGAC GGCTGTCGCA TCCATGTCAT TGACCACAGT 
GGCGTCCGGC TCGGAGAGTA TGAGGATGTG TCCAAAGTGG AGAAATACGA GATCTCACCA 
GAAGCTTACG AACGGAGGCA GAACACAGTT CGATCCTTCA TGAAACGCAG CAAGCTAGGC 
CCGTACAATG AAGAACTCCG GGCCCAGCAA GAGGCAGAGG CTGCCCAGCG TCTCAGTGAG 
GAGAAGGCCC AAGCCAGTGC CATCTCTGTG GGGAGCCGTT GTGAGGTGCG GGCACCCGAC 
CACTCCCTTC GCAGGGGCAC TGTCATGTAT GTAGGACTCA CAGATTTCAA GCCAGGCTAC 
TGGGTTGGTG TCCGATATGA TGAGCCGTTA GGAAAAAACG ATGGCAGTGT GAATGGGAAA 
CGCTACTTTG AATGCCAGGC CAAGTATGGT GCCTTTGTAA AGCCATCAGC TGTCACCGTA 
GGAGATTTCC CAGAGGAGGA CTACGGGTTG GACGAGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248974.1
CDS	160..741
Translation	MELELYGADDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQQEAEAAQRLSEEKAQASAISVGSRCEVRAPDHSLRRGTVMYVGLTDFKPGYWVGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSAVTVGDFPEEDYGLDEM

Target ORF information:

RefSeq Version	XM_011250672.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin folding cofactor B (Tbcb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250672.2

ORF Insert Sequence:

ATGGAACTGG AGCTATATGG AGCAGACGAC AAGTTCTACA GCAAGTTGGA TCAGGAGGAT 
GCACTGCTGG GCTCCTATCC TGTGGATGAC GGCTGTCGCA TCCATGTCAT TGACCACAGT 
GGCGTCCGGC TCGGAGAGTA TGAGGATGTG TCCAAAGTGG AGAAATACGA GATCTCACCA 
GAAGCTTACG AACGGAGGCA GAACACAGTT CGATCCTTCA TGAAACGCAG CAAGCTAGGC 
CCGTACAATG AAGAACTCCG GGCCCAGCAA GAGGCAGAGG CTGCCCAGCG TCTCAGTGAG 
GAGAAGGCCC AAGCCAGTGC CATCTCTGTG GGGAGCCGTT GTGAGGTGCG GGCACCCGAC 
CACTCCCTTC GCAGGGGCAC TGTCATGTAT GTAGGACTCA CAGATTTCAA GCCAGGCTAC 
TGGGTTGGTG TCCGATATGA TGAGCCGTTA GGAAAAAACG ATGGCAGTGT GAATGGGAAA 
CGCTACTTTG AATGCCAGGC CAAGTATGGT GCCTTTGTAA AGCCATCAGC TGTCACCGTA 
GGAGATTTCC CAGAGGAGGA CTACGGGTTG GACGAGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbcb ( NM_025548.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079824.2 Mus musculus tubulin folding cofactor B (Tbcb), mRNA.

Price & Availability↑

CloneID	OMu00654
Clone ID Related Accession (Same CDS sequence)	NM_025548.3 , NM_025548.4
Accession Version	NM_025548.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Mus musculus(house mouse)
Product	tubulin-folding cofactor B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ445322.1 and AK002316.1. On Mar 21, 2008 this sequence version replaced NM_025548.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.423838.1, SRR1660815.262773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN00849389 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTGA CGGGGATATC GGCGCCCACC GTGATGGTTT TCATCAGCAG CTCCCTCAAC 
AGCTTCCGTT CGGAGAAGCG ATACAGTCGT AGCCTCACCA TCGCAGAGTT CAAGTGTAAA 
CTGGAGCTGG TGGTGGGCAG CCCTGCTTCC TGCATGGAAC TGGAGCTATA TGGAGCAGAC 
GACAAGTTCT ACAGCAAGTT GGATCAGGAG GATGCACTGC TGGGCTCCTA TCCTGTGGAT 
GACGGCTGTC GCATCCATGT CATTGACCAC AGTGGCGTCC GGCTCGGAGA GTATGAGGAT 
GTGTCCAAAG TGGAGAAATA CGAGATCTCA CCAGAAGCTT ACGAACGGAG GCAGAACACA 
GTTCGATCCT TCATGAAACG CAGCAAGCTA GGCCCGTACA ATGAAGAACT CCGGGCCCAG 
CAAGAGGCAG AGGCTGCCCA GCGTCTCAGT GAGGAGAAGG CCCAAGCCAG TGCCATCTCT 
GTGGGGAGCC GTTGTGAGGT GCGGGCACCC GACCACTCCC TTCGCAGGGG CACTGTCATG 
TATGTAGGAC TCACAGATTT CAAGCCAGGC TACTGGGTTG GTGTCCGATA TGATGAGCCG 
TTAGGAAAAA ACGATGGCAG TGTGAATGGG AAACGCTACT TTGAATGCCA GGCCAAGTAT 
GGTGCCTTTG TAAAGCCATC AGCTGTCACC GTAGGAGATT TCCCAGAGGA GGACTACGGG 
TTGGACGAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079824.2
CDS	337..1071
Translation	MEVTGISAPTVMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELVVGSPASCMELELYGADDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQQEAEAAQRLSEEKAQASAISVGSRCEVRAPDHSLRRGTVMYVGLTDFKPGYWVGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSAVTVGDFPEEDYGLDEM

Target ORF information:

RefSeq Version	NM_025548.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin folding cofactor B (Tbcb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025548.3

ORF Insert Sequence:

ATGGAGGTGA CGGGGATATC GGCGCCCACC GTGATGGTTT TCATCAGCAG CTCCCTCAAC 
AGCTTCCGTT CGGAGAAGCG ATACAGTCGT AGCCTCACCA TCGCAGAGTT CAAGTGTAAA 
CTGGAGCTGG TGGTGGGCAG CCCTGCTTCC TGCATGGAAC TGGAGCTATA TGGAGCAGAC 
GACAAGTTCT ACAGCAAGTT GGATCAGGAG GATGCACTGC TGGGCTCCTA TCCTGTGGAT 
GACGGCTGTC GCATCCATGT CATTGACCAC AGTGGCGTCC GGCTCGGAGA GTATGAGGAT 
GTGTCCAAAG TGGAGAAATA CGAGATCTCA CCAGAAGCTT ACGAACGGAG GCAGAACACA 
GTTCGATCCT TCATGAAACG CAGCAAGCTA GGCCCGTACA ATGAAGAACT CCGGGCCCAG 
CAAGAGGCAG AGGCTGCCCA GCGTCTCAGT GAGGAGAAGG CCCAAGCCAG TGCCATCTCT 
GTGGGGAGCC GTTGTGAGGT GCGGGCACCC GACCACTCCC TTCGCAGGGG CACTGTCATG 
TATGTAGGAC TCACAGATTT CAAGCCAGGC TACTGGGTTG GTGTCCGATA TGATGAGCCG 
TTAGGAAAAA ACGATGGCAG TGTGAATGGG AAACGCTACT TTGAATGCCA GGCCAAGTAT 
GGTGCCTTTG TAAAGCCATC AGCTGTCACC GTAGGAGATT TCCCAGAGGA GGACTACGGG 
TTGGACGAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbcb ( NM_025548.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079824.2 Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00654
Clone ID Related Accession (Same CDS sequence)	NM_025548.3 , NM_025548.4
Accession Version	NM_025548.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	tubulin-folding cofactor B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149067.3. On Feb 8, 2018 this sequence version replaced NM_025548.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.299878.1, SRR1660817.257349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN00849389 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGA CGGGGATATC GGCGCCCACC GTGATGGTTT TCATCAGCAG CTCCCTCAAC 
AGCTTCCGTT CGGAGAAGCG ATACAGTCGT AGCCTCACCA TCGCAGAGTT CAAGTGTAAA 
CTGGAGCTGG TGGTGGGCAG CCCTGCTTCC TGCATGGAAC TGGAGCTATA TGGAGCAGAC 
GACAAGTTCT ACAGCAAGTT GGATCAGGAG GATGCACTGC TGGGCTCCTA TCCTGTGGAT 
GACGGCTGTC GCATCCATGT CATTGACCAC AGTGGCGTCC GGCTCGGAGA GTATGAGGAT 
GTGTCCAAAG TGGAGAAATA CGAGATCTCA CCAGAAGCTT ACGAACGGAG GCAGAACACA 
GTTCGATCCT TCATGAAACG CAGCAAGCTA GGCCCGTACA ATGAAGAACT CCGGGCCCAG 
CAAGAGGCAG AGGCTGCCCA GCGTCTCAGT GAGGAGAAGG CCCAAGCCAG TGCCATCTCT 
GTGGGGAGCC GTTGTGAGGT GCGGGCACCC GACCACTCCC TTCGCAGGGG CACTGTCATG 
TATGTAGGAC TCACAGATTT CAAGCCAGGC TACTGGGTTG GTGTCCGATA TGATGAGCCG 
TTAGGAAAAA ACGATGGCAG TGTGAATGGG AAACGCTACT TTGAATGCCA GGCCAAGTAT 
GGTGCCTTTG TAAAGCCATC AGCTGTCACC GTAGGAGATT TCCCAGAGGA GGACTACGGG 
TTGGACGAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079824.2
CDS	505..1239
Translation	MEVTGISAPTVMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELVVGSPASCMELELYGADDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQQEAEAAQRLSEEKAQASAISVGSRCEVRAPDHSLRRGTVMYVGLTDFKPGYWVGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSAVTVGDFPEEDYGLDEM

Target ORF information:

RefSeq Version	NM_025548.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025548.4

ORF Insert Sequence:

ATGGAGGTGA CGGGGATATC GGCGCCCACC GTGATGGTTT TCATCAGCAG CTCCCTCAAC 
AGCTTCCGTT CGGAGAAGCG ATACAGTCGT AGCCTCACCA TCGCAGAGTT CAAGTGTAAA 
CTGGAGCTGG TGGTGGGCAG CCCTGCTTCC TGCATGGAAC TGGAGCTATA TGGAGCAGAC 
GACAAGTTCT ACAGCAAGTT GGATCAGGAG GATGCACTGC TGGGCTCCTA TCCTGTGGAT 
GACGGCTGTC GCATCCATGT CATTGACCAC AGTGGCGTCC GGCTCGGAGA GTATGAGGAT 
GTGTCCAAAG TGGAGAAATA CGAGATCTCA CCAGAAGCTT ACGAACGGAG GCAGAACACA 
GTTCGATCCT TCATGAAACG CAGCAAGCTA GGCCCGTACA ATGAAGAACT CCGGGCCCAG 
CAAGAGGCAG AGGCTGCCCA GCGTCTCAGT GAGGAGAAGG CCCAAGCCAG TGCCATCTCT 
GTGGGGAGCC GTTGTGAGGT GCGGGCACCC GACCACTCCC TTCGCAGGGG CACTGTCATG 
TATGTAGGAC TCACAGATTT CAAGCCAGGC TACTGGGTTG GTGTCCGATA TGATGAGCCG 
TTAGGAAAAA ACGATGGCAG TGTGAATGGG AAACGCTACT TTGAATGCCA GGCCAAGTAT 
GGTGCCTTTG TAAAGCCATC AGCTGTCACC GTAGGAGATT TCCCAGAGGA GGACTACGGG 
TTGGACGAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbcb ( NM_001360649.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347578.1 Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84327
Clone ID Related Accession (Same CDS sequence)	NM_001360649.1
Accession Version	NM_001360649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	tubulin-folding cofactor B isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149067.3 and AA718358.1. Transcript Variant: This variant (3) uses an alternate splice junction compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849386, SAMN00849389 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGA CGGGGATATC GGCGCCCACC GTGATGGTTT TCATCAGCAG CTCCCTCAAC 
AGCTTCCGTT CGGAGAAGCG ATACAGTCGT AGCCTCACCA TCGCAGAGTT CAAGTGTAAA 
CTGGAGCTGG TGGTGGGCAG CCCTGCTTCC TGCATGGAAC TGGAGCTATA TGGAGCAGAC 
GACAAGTTCT ACAGCAAGTT GGATCAGGAG GATGCACTGC TGGGCTCCTA TCCTGTGGAT 
GACGGCTGTC GCATCCATGT CATTGACCAC AGTGGCGTCC GGCTCGGAGA GTATGAGGAT 
GTGTCCAAAG TGGAGAAATA CGAGATCTCA CCAGAAGCTT ACGAACGGAG GCAGAACACA 
GTTCGATCCT TCATGAAACG CAGCAAGCTA GGCCCGTACA ATGAAGAACT CCGGGCCCAG 
CAAGAGGCAG AGGCTGCCCA GCGTCTCAGT GAGGAGAAGG CCCAAGCCAG TGCCATCTCT 
GTGGGGAGCC GTTGTGAGGA CTCACAGATT TCAAGCCAGG CTACTGGGTT GGTGTCCGAT 
ATGATGAGCC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347578.1
CDS	505..1059
Translation	MEVTGISAPTVMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELVVGSPASCMELELYGADDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQQEAEAAQRLSEEKAQASAISVGSRCEDSQISSQATGLVSDMMSR

Target ORF information:

RefSeq Version	NM_001360649.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin folding cofactor B (Tbcb), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360649.1

ORF Insert Sequence:

ATGGAGGTGA CGGGGATATC GGCGCCCACC GTGATGGTTT TCATCAGCAG CTCCCTCAAC 
AGCTTCCGTT CGGAGAAGCG ATACAGTCGT AGCCTCACCA TCGCAGAGTT CAAGTGTAAA 
CTGGAGCTGG TGGTGGGCAG CCCTGCTTCC TGCATGGAAC TGGAGCTATA TGGAGCAGAC 
GACAAGTTCT ACAGCAAGTT GGATCAGGAG GATGCACTGC TGGGCTCCTA TCCTGTGGAT 
GACGGCTGTC GCATCCATGT CATTGACCAC AGTGGCGTCC GGCTCGGAGA GTATGAGGAT 
GTGTCCAAAG TGGAGAAATA CGAGATCTCA CCAGAAGCTT ACGAACGGAG GCAGAACACA 
GTTCGATCCT TCATGAAACG CAGCAAGCTA GGCCCGTACA ATGAAGAACT CCGGGCCCAG 
CAAGAGGCAG AGGCTGCCCA GCGTCTCAGT GAGGAGAAGG CCCAAGCCAG TGCCATCTCT 
GTGGGGAGCC GTTGTGAGGA CTCACAGATT TCAAGCCAGG CTACTGGGTT GGTGTCCGAT 
ATGATGAGCC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tubulin-binding cofactor B is a direct interaction partner of the dynactin subunit p150(Glued).
Cell and tissue research350(1)13-26(2012 Oct)
Kuh GF,Stockmann M,Meyer-Ohlendorf M,Linta L,Proepper C,Ludolph AC,Bockmann J,Boeckers TM,Liebau S

RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly.
PLoS genetics8(10)e1002969(2012)
Lo JC,Jamsai D,O'Connor AE,Borg C,Clark BJ,Whisstock JC,Field MC,Adams V,Ishikawa T,Aitken RJ,Whittle B,Goodnow CC,Ormandy CJ,O'Bryan MK

Stathmin, a microtubule-destabilizing protein, is dysregulated in spinal muscular atrophy.
Human molecular genetics19(9)1766-78(2010 May)
Wen HL,Lin YT,Ting CH,Lin-Chao S,Li H,Hsieh-Li HM

Tubulin cofactor B plays a role in the neuronal growth cone.
Journal of neurochemistry100(6)1680-7(2007 Mar)
Lopez-Fanarraga M,Carranza G,Bellido J,Kortazar D,Villegas JC,Zabala JC

Role of cofactors B (TBCB) and E (TBCE) in tubulin heterodimer dissociation.
Experimental cell research313(3)425-36(2007 Feb)
Kortazar D,Fanarraga ML,Carranza G,Bellido J,Villegas JC,Avila J,Zabala JC

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Tbcb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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