Osgep cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Osgep
Entrez Gene ID	66246
Full Name	O-sialoglycoprotein endopeptidase
Synonyms	1500019L24Rik,GCPL-1,PRSMG1
General protein information	Preferred Names O-sialoglycoprotein endopeptidase Names probable tRNA N6-adenosine threonylcarbamoyltransferase N6-L-threonylcarbamoyladenine synthase mOsgep probable O-sialoglycoprotein endopeptidase probable tRNA threonylcarbamoyladenosine biosynthesis protein Osgep t(6)A synthase t(6)A37 threonylcarbamoyladenosine biosynthesis protein Osgep tRNA threonylcarbamoyladenosine biosynthesis protein Osgep
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_133676.2	NP_598437.2	probable tRNA N6-adenosine threonylcarbamoyltransferase

Pathways from BioSystems

Homologies

Homo sapiens (human)	OSGEP	NP_060277.1
Canis lupus familiaris (dog)	OSGEP	XP_003432020.2
Danio rerio (zebrafish)	osgep	NP_001017751.2
Caenorhabditis elegans (roundworm)	Y71H2AM.1	NP_497625.3
Kluyveromyces lactis	KLLA0F21450g	XP_456041.1
Schizosaccharomyces pombe (fission yeast)	pgp2	NP_596498.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006952	XP_308804.2
Arabidopsis thaliana (thale cress)	AT4G22720	NP_974593.1
Xenopus tropicalis (tropical clawed frog)	osgep	NP_001016112.1
	OSGEP	XP_001088971.1
Bos taurus (cattle)	OSGEP	NP_001068787.1
Saccharomyces cerevisiae (baker's yeast)	KAE1	NP_012964.2
Pan troglodytes (chimpanzee)	OSGEP	XP_001139005.1
Rattus norvegicus (Norway rat)	Osgep	NP_001093980.1
Magnaporthe oryzae (rice blast fungus)	MGG_06931	XP_003709670.1
Mus musculus (house mouse)	Osgep	NP_598437.2
Drosophila melanogaster (fruit fly)	CG4933	NP_648880.1
Eremothecium gossypii	AGOS_AEL316W	NP_984544.1
Neurospora crassa	NCU03836	XP_960595.1
Oryza sativa (rice)	Os05g0194600	NP_001054858.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly.
Braun DA, Rao J, Mollet G, Schapiro D, Daugeron MC, Tan W, Gribouval O, Boyer O, Revy P, Jobst-Schwan T, Schmidt JM, Lawson JA, Schanze D, Ashraf S, Ullmann JFP, Hoogstraten CA, Boddaert N, Collinet B, Martin G, Liger D, Lovric S, Furlano M, Guerrera IC, Sanchez-Ferras O, Hu JF, Boschat AC, Sanquer S, Menten B, Vergult S, De Rocker N, Airik M, Hermle T, Shril S, Widmeier E, Gee HY, Choi WI, Sadowski CE, Pabst WL, Warejko JK, Daga A, Basta T, Matejas V, Scharmann K, Kienast SD, Behnam B, Beeson B, Begtrup A, Bruce M, Ch'ng GS, Lin SP, Chang JH, Chen CH, Cho MT, Gaffney PM, Gipson PE, Hsu CH, Kari JA, Ke YY, Kiraly-Borri C, Lai WM, Lemyre E, Littlejohn RO, Masri A, Moghtaderi M, Nakamura K, Ozaltin F, Praet M, Prasad C, Prytula A, Roeder ER, Rump P, Schnur RE, Shiihara T, Sinha MD, Soliman NA, Soulami K, Sweetser DA, Tsai WH, Tsai JD, Topaloglu R, Vester U, Viskochil DH, Vatanavicharn N, Waxler JL, Wierenga KJ, Wolf MTF, Wong SN, Leidel SA, Truglio G, Dedon PC, Poduri A, Mane S, Lifton RP, Bouchard M, Kannu P, Chitayat D, Magen D, Callewaert B, van Tilbeurgh H, Zenker M, Antignac C, Hildebrandt F
Nature genetics49(10)1529-1538(2017 Oct)

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Tang F, Barbacioru C, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA
PloS one6(6)e21208(2011)

Identification of the functional elements in the bidirectional promoter of the mouse O-sialoglycoprotein endopeptidase and APEX nuclease genes.
Ikeda S, Ayabe H, Mori K, Seki Y, Seki S
Biochemical and biophysical research communications296(4)785-91(2002 Aug)

Sequencing analysis of a putative human O-sialoglycoprotein endopeptidase gene (OSGEP) and analysis of a bidirectional promoter between the OSGEP and APEX genes.
Seki Y, Ikeda S, Kiyohara H, Ayabe H, Seki T, Matsui H
Gene285(1-2)101-8(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

promoter regions and tissue distribution of Osgep
Title: Identification of the functional elements in the bidirectional promoter of the mouse O-sialoglycoprotein endopeptidase and APEX nuclease genes.

The following Osgep gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Osgep cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23370	NM_133676.2 Latest version!	Mus musculus O-sialoglycoprotein endopeptidase (Osgep), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Osgep ( NM_133676.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598437.2 Mus musculus O-sialoglycoprotein endopeptidase (Osgep), mRNA.

Price & Availability↑

CloneID	OMu23370
Clone ID Related Accession (Same CDS sequence)	NM_133676.2
Accession Version	NM_133676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	probable tRNA N6-adenosine threonylcarbamoyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA481753.1 and AK142730.1. On Jan 10, 2006 this sequence version replaced NM_133676.1. ##Evidence-Data-START## Transcript exon combination :: AK142730.1, AK157554.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGCCCGCGG TGCTGGGGTT CGAAGGCAGC GCCAACAAGA TCGGCGTGGG CGTGGTCCGC 
GACGGCACGG TGCTGGCGAA CCCGCGGCGC ACTTACGTCA CGGCCCCGGG CACCGGATTC 
CTTCCAGGTG ACACGGCCAG GCACCATCGA GCTGTTATCC TAGACCTACT GCAGGAGGCG 
CTAACAGAGG CAGGATTGAC CTCCAAGGAC ATTGATTGTA TTGCTTTCAC CAAAGGTCCT 
GGCATGGGAT CCCCATTGGC TTCTGTAGCT GTTGTTGCCC GTACAGTGGC CCAGTTGTGG 
AATAAGCCTT TGCTTGGCGT GAACCACTGC ATAGGCCACA TTGAAATGGG CCGTCTCATC 
ACTGGAGCCG TTAACCCAAC TGTCCTGTAT GTGAGCGGAG GAAATACCCA GGTGATTTCC 
TACTCAGAAC ATCGTTATCG CATCTTTGGA GAAACTATTG ATATCGCCGT GGGAAACTGC 
CTGGATCGTT TTGCTCGGGT GCTGAAGATT TCCAATGACC CCAGTCCAGG CTACAACATT 
GAGCAGATGG CAAAGCGAGG CAAGAAGCTA GTCGAGCTGC CATACACTGT AAAGGGGATG 
GATGTCTCGT TTTCAGGGAT TCTGTCTTTC ATTGAGGATG CAGCGCAGCG AATGCTGGCC 
ACTGGAGAGT GTACTCCTGA AGACCTGTGT TTCTCCTTAC AGGAAACCGT GTTTGCAATG 
CTAGTGGAAA TCACGGAGCG AGCCATGGCA CACTGTGGCT CCAAGGAAGC CCTCATCGTC 
GGAGGAGTTG GATGTAACCT GAGGCTGCAG GAGATGATGG GGACAATGTG CCAGGAGCGG 
GGAGCCCAGC TCTTTGCAAC AGATGAGAGA TTCTGCGTTG ACAATGGAGC CATGATAGCC 
CAAGCTGGTT GGGAGATGTT TCAGGCTGGG CACAGGACTC CTCTCAAAGA TTCTGCAATT 
ACTCAGAGGT ATAGGACAGA TGAAGTGGAA GTGACATGGA GGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598437.2
CDS	116..1123
Translation	MPAVLGFEGSANKIGVGVVRDGTVLANPRRTYVTAPGTGFLPGDTARHHRAVILDLLQEALTEAGLTSKDIDCIAFTKGPGMGSPLASVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGAVNPTVLYVSGGNTQVISYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMDVSFSGILSFIEDAAQRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSKEALIVGGVGCNLRLQEMMGTMCQERGAQLFATDERFCVDNGAMIAQAGWEMFQAGHRTPLKDSAITQRYRTDEVEVTWRD

Target ORF information:

RefSeq Version	NM_133676.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus O-sialoglycoprotein endopeptidase (Osgep), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133676.2

ORF Insert Sequence:

ATGCCCGCGG TGCTGGGGTT CGAAGGCAGC GCCAACAAGA TCGGCGTGGG CGTGGTCCGC 
GACGGCACGG TGCTGGCGAA CCCGCGGCGC ACTTACGTCA CGGCCCCGGG CACCGGATTC 
CTTCCAGGTG ACACGGCCAG GCACCATCGA GCTGTTATCC TAGACCTACT GCAGGAGGCG 
CTAACAGAGG CAGGATTGAC CTCCAAGGAC ATTGATTGTA TTGCTTTCAC CAAAGGTCCT 
GGCATGGGAT CCCCATTGGC TTCTGTAGCT GTTGTTGCCC GTACAGTGGC CCAGTTGTGG 
AATAAGCCTT TGCTTGGCGT GAACCACTGC ATAGGCCACA TTGAAATGGG CCGTCTCATC 
ACTGGAGCCG TTAACCCAAC TGTCCTGTAT GTGAGCGGAG GAAATACCCA GGTGATTTCC 
TACTCAGAAC ATCGTTATCG CATCTTTGGA GAAACTATTG ATATCGCCGT GGGAAACTGC 
CTGGATCGTT TTGCTCGGGT GCTGAAGATT TCCAATGACC CCAGTCCAGG CTACAACATT 
GAGCAGATGG CAAAGCGAGG CAAGAAGCTA GTCGAGCTGC CATACACTGT AAAGGGGATG 
GATGTCTCGT TTTCAGGGAT TCTGTCTTTC ATTGAGGATG CAGCGCAGCG AATGCTGGCC 
ACTGGAGAGT GTACTCCTGA AGACCTGTGT TTCTCCTTAC AGGAAACCGT GTTTGCAATG 
CTAGTGGAAA TCACGGAGCG AGCCATGGCA CACTGTGGCT CCAAGGAAGC CCTCATCGTC 
GGAGGAGTTG GATGTAACCT GAGGCTGCAG GAGATGATGG GGACAATGTG CCAGGAGCGG 
GGAGCCCAGC TCTTTGCAAC AGATGAGAGA TTCTGCGTTG ACAATGGAGC CATGATAGCC 
CAAGCTGGTT GGGAGATGTT TCAGGCTGGG CACAGGACTC CTCTCAAAGA TTCTGCAATT 
ACTCAGAGGT ATAGGACAGA TGAAGTGGAA GTGACATGGA GGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly.
Nature genetics49(10)1529-1538(2017 Oct)
Braun DA,Rao J,Mollet G,Schapiro D,Daugeron MC,Tan W,Gribouval O,Boyer O,Revy P,Jobst-Schwan T,Schmidt JM,Lawson JA,Schanze D,Ashraf S,Ullmann JFP,Hoogstraten CA,Boddaert N,Collinet B,Martin G,Liger D,Lovric S,Furlano M,Guerrera IC,Sanchez-Ferras O,Hu JF,Boschat AC,Sanquer S,Menten B,Vergult S,De Rocker N,Airik M,Hermle T,Shril S,Widmeier E,Gee HY,Choi WI,Sadowski CE,Pabst WL,Warejko JK,Daga A,Basta T,Matejas V,Scharmann K,Kienast SD,Behnam B,Beeson B,Begtrup A,Bruce M,Ch'ng GS,Lin SP,Chang JH,Chen CH,Cho MT,Gaffney PM,Gipson PE,Hsu CH,Kari JA,Ke YY,Kiraly-Borri C,Lai WM,Lemyre E,Littlejohn RO,Masri A,Moghtaderi M,Nakamura K,Ozaltin F,Praet M,Prasad C,Prytula A,Roeder ER,Rump P,Schnur RE,Shiihara T,Sinha MD,Soliman NA,Soulami K,Sweetser DA,Tsai WH,Tsai JD,Topaloglu R,Vester U,Viskochil DH,Vatanavicharn N,Waxler JL,Wierenga KJ,Wolf MTF,Wong SN,Leidel SA,Truglio G,Dedon PC,Poduri A,Mane S,Lifton RP,Bouchard M,Kannu P,Chitayat D,Magen D,Callewaert B,van Tilbeurgh H,Zenker M,Antignac C,Hildebrandt F

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
PloS one6(6)e21208(2011)
Tang F,Barbacioru C,Nordman E,Bao S,Lee C,Wang X,Tuch BB,Heard E,Lao K,Surani MA

Identification of the functional elements in the bidirectional promoter of the mouse O-sialoglycoprotein endopeptidase and APEX nuclease genes.
Biochemical and biophysical research communications296(4)785-91(2002 Aug)
Ikeda S,Ayabe H,Mori K,Seki Y,Seki S

Sequencing analysis of a putative human O-sialoglycoprotein endopeptidase gene (OSGEP) and analysis of a bidirectional promoter between the OSGEP and APEX genes.
Gene285(1-2)101-8(2002 Feb)
Seki Y,Ikeda S,Kiyohara H,Ayabe H,Seki T,Matsui H

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Osgep cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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