Eif3f cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif3f
Entrez Gene ID	66085
Full Name	eukaryotic translation initiation factor 3, subunit F
Synonyms	0610037M02Rik,AA409853,Eif3s5
General protein information	Preferred Names eukaryotic translation initiation factor 3, subunit F Names eukaryotic translation initiation factor 3 subunit F deubiquitinating enzyme eIF3f eIF-3-epsilon eIF3 p47 eIF3f eukaryotic translation initiation factor 3, subunit 5 (epsilon)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_025344.2	NP_079620.2	eukaryotic translation initiation factor 3 subunit F

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	eif-3.F	NP_495988.1
Neurospora crassa	NCU01021	XP_961633.1
Xenopus tropicalis (tropical clawed frog)	eif3f	NP_001016190.1
Homo sapiens (human)	EIF3F	NP_003745.1
	EIF3F	XP_001105893.2
Drosophila melanogaster (fruit fly)	CG9769	NP_649489.1
Anopheles gambiae (African malaria mosquito)	EIF3F_ANOGA	XP_311967.3
Pan troglodytes (chimpanzee)	EIF3F	NP_001104282.1
Gallus gallus (chicken)	EIF3F	XP_421624.1
Schizosaccharomyces pombe (fission yeast)	SPBC4C3.07	NP_596298.1
Arabidopsis thaliana (thale cress)	EIF2	NP_181528.1
Canis lupus familiaris (dog)	EIF3F	XP_534044.4
Rattus norvegicus (Norway rat)	Eif3f	NP_001264231.1
Danio rerio (zebrafish)	eif3f	NP_001186938.1
Oryza sativa (rice)	Os05g0104800	NP_001054405.1
Bos taurus (cattle)	EIF3F	XP_002693111.1
Mus musculus (house mouse)	Eif3f	NP_079620.2
Magnaporthe oryzae (rice blast fungus)	MGG_10653	XP_003710082.1

Gene Ontology
Bibliography

Related articles in PubMed

The m subunit of murine translation initiation factor eIF3 maintains the integrity of the eIF3 complex and is required for embryonic development, homeostasis, and organ size control.
Zeng L, Wan Y, Li D, Wu J, Shao M, Chen J, Hui L, Ji H, Zhu X
The Journal of biological chemistry288(42)30087-93(2013 Oct)

The translation initiation factor 3f (eIF3f) exhibits a deubiquitinase activity regulating Notch activation.
Moretti J, Chastagner P, Gastaldello S, Heuss SF, Dirac AM, Bernards R, Masucci MG, Isra?l A, Brou C
PLoS biology8(11)e1000545(2010 Nov)

The translation regulatory subunit eIF3f controls the kinase-dependent mTOR signaling required for muscle differentiation and hypertrophy in mouse.
Csibi A, Cornille K, Leibovitch MP, Poupon A, Tintignac LA, Sanchez AM, Leibovitch SA
PloS one5(2)e8994(2010 Feb)

Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease.
Tkatchenko TV, Moreno-Rodriguez RA, Conway SJ, Molkentin JD, Markwald RR, Tkatchenko AV
Physiological genomics39(3)160-8(2009 Nov)

MAFbx/Atrogin-1 controls the activity of the initiation factor eIF3-f in skeletal muscle atrophy by targeting multiple C-terminal lysines.
Csibi A, Leibovitch MP, Cornille K, Tintignac LA, Leibovitch SA
The Journal of biological chemistry284(7)4413-21(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data provide evidence that in skeletal muscle cells the degradation of eIF3f suppresses S6K1 activation by mTOR signaling.
Title: The translation regulatory subunit eIF3f controls the kinase-dependent mTOR signaling required for muscle differentiation and hypertrophy in mouse.

MAFbx/Atrogin-1 controls the activity of the initiation factor eIF3-f in skeletal muscle atrophy by targeting multiple C-terminal lysines.
Title: MAFbx/Atrogin-1 controls the activity of the initiation factor eIF3-f in skeletal muscle atrophy by targeting multiple C-terminal lysines.

Key target that accounts for E3 ubiquitin ligase MAFbx (atrogin1, Fbxo32) function during muscle atrophy; has a major role in skeletal muscle hypertrophy.
Title: The initiation factor eIF3-f is a major target for atrogin1/MAFbx function in skeletal muscle atrophy.

The following Eif3f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif3f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18176	NM_025344.2 Latest version!	Mus musculus eukaryotic translation initiation factor 3, subunit F (Eif3f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif3f ( NM_025344.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079620.2 Mus musculus eukaryotic translation initiation factor 3, subunit F (Eif3f), mRNA.

Price & Availability↑

CloneID	OMu18176
Clone ID Related Accession (Same CDS sequence)	NM_025344.2
Accession Version	NM_025344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 3 subunit F
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK152037.1, BC083190.1 and AK136797.1. On Mar 25, 2009 this sequence version replaced NM_025344.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.274129.1, AK136797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTCTC CGGCCGTACC GGCTAATGTC CCTCCTGCCA CTGCAGCCGC AGCCCCGGCG 
CCGGTCGTCA CCGCAGCCCC GGCTTCAGCC CCGACCCCAT CCACGCCAGC TCCGACACCG 
GCTGCGACTC CCGCTGCGTC CCCGGCGCCC GTCTCGTCTG ATCCTGCTGT AGCTGCGCCT 
GCAGCCCCGG GCCAGACCCC AGCCTCCGCG CCAGCCCCAG CGCAGACGCC GGCGCCTTCG 
CAGCCCGGGC CCGCCCTCCC GGGGCCTTTC CCGGGCGGCC GCGTGGTCAG GCTACACCCC 
GTCATTTTGG CCTCGATCGT GGACAGCTAC GAACGCCGCA ACGAGGGAGC TGCCCGAGTT 
ATTGGAACCC TGTTGGGAAC TGTTGACAAG CACTCGGTAG AAGTCACCAA CTGCTTTTCG 
GTGCCACACA ATGAGTCAGA AGATGAAGTG GCTGTTGACA TGGAATTTGC TAAGAATATG 
TATGAATTAC ATAAAAAAGT CTCCCCAAAT GAGCTCATCC TAGGCTGGTA TGCCACAGGC 
CATGACATCA CAGAACACTC AGTGCTGATC CATGAGTACT ACAGCAGGGA GGCCCCGAAC 
CCCATTCACC TCACGGTGGA CACAGGTCTC CAGCATGGGC GCATGAGCAT CAAGGCCTAT 
GTCAGCACTT TAATGGGTGT CCCTGGGAGG ACCATGGGAG TGATGTTCAC ACCTCTCACA 
GTGAAGTACG CGTATTATGA CACTGAACGC ATTGGAGTTG ACCTCATCAT GAAGACGTGT 
TTTAGCCCCA ACCGGGTGAT TGGACTCTCA AGTGACTTAC AACAAGTGGG AGGGGCCTCA 
GCTCGCATCC AGGATGCTCT AAGCACTGTA TTACAGTATG CTGAGGATGT GCTGTCTGGG 
AAAGTGTCTG CTGACAACAC GGTGGGCCGC TTCTTGATGA GCCTTGTCAA CCAAGTACCC 
AAGATAGTTC CTGATGACTT TGAGACCATG CTCAACAGCA ACATCAATGA CCTGCTGATG 
GTGACCTACC TGGCCAATCT CACCCAGTCA CAGATTGCCC TCAACGAGAA ACTTGTAAAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079620.2
CDS	18..1103
Translation	MASPAVPANVPPATAAAAPAPVVTAAPASAPTPSTPAPTPAATPAASPAPVSSDPAVAAPAAPGQTPASAPAPAQTPAPSQPGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYATGHDITEHSVLIHEYYSREAPNPIHLTVDTGLQHGRMSIKAYVSTLMGVPGRTMGVMFTPLTVKYAYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVSADNTVGRFLMSLVNQVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIALNEKLVNL

Target ORF information:

RefSeq Version	NM_025344.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 3, subunit F (Eif3f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025344.2

ORF Insert Sequence:

ATGGCTTCTC CGGCCGTACC GGCTAATGTC CCTCCTGCCA CTGCAGCCGC AGCCCCGGCG 
CCGGTCGTCA CCGCAGCCCC GGCTTCAGCC CCGACCCCAT CCACGCCAGC TCCGACACCG 
GCTGCGACTC CCGCTGCGTC CCCGGCGCCC GTCTCGTCTG ATCCTGCTGT AGCTGCGCCT 
GCAGCCCCGG GCCAGACCCC AGCCTCCGCG CCAGCCCCAG CGCAGACGCC GGCGCCTTCG 
CAGCCCGGGC CCGCCCTCCC GGGGCCTTTC CCGGGCGGCC GCGTGGTCAG GCTACACCCC 
GTCATTTTGG CCTCGATCGT GGACAGCTAC GAACGCCGCA ACGAGGGAGC TGCCCGAGTT 
ATTGGAACCC TGTTGGGAAC TGTTGACAAG CACTCGGTAG AAGTCACCAA CTGCTTTTCG 
GTGCCACACA ATGAGTCAGA AGATGAAGTG GCTGTTGACA TGGAATTTGC TAAGAATATG 
TATGAATTAC ATAAAAAAGT CTCCCCAAAT GAGCTCATCC TAGGCTGGTA TGCCACAGGC 
CATGACATCA CAGAACACTC AGTGCTGATC CATGAGTACT ACAGCAGGGA GGCCCCGAAC 
CCCATTCACC TCACGGTGGA CACAGGTCTC CAGCATGGGC GCATGAGCAT CAAGGCCTAT 
GTCAGCACTT TAATGGGTGT CCCTGGGAGG ACCATGGGAG TGATGTTCAC ACCTCTCACA 
GTGAAGTACG CGTATTATGA CACTGAACGC ATTGGAGTTG ACCTCATCAT GAAGACGTGT 
TTTAGCCCCA ACCGGGTGAT TGGACTCTCA AGTGACTTAC AACAAGTGGG AGGGGCCTCA 
GCTCGCATCC AGGATGCTCT AAGCACTGTA TTACAGTATG CTGAGGATGT GCTGTCTGGG 
AAAGTGTCTG CTGACAACAC GGTGGGCCGC TTCTTGATGA GCCTTGTCAA CCAAGTACCC 
AAGATAGTTC CTGATGACTT TGAGACCATG CTCAACAGCA ACATCAATGA CCTGCTGATG 
GTGACCTACC TGGCCAATCT CACCCAGTCA CAGATTGCCC TCAACGAGAA ACTTGTAAAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The m subunit of murine translation initiation factor eIF3 maintains the integrity of the eIF3 complex and is required for embryonic development, homeostasis, and organ size control.
The Journal of biological chemistry288(42)30087-93(2013 Oct)
Zeng L,Wan Y,Li D,Wu J,Shao M,Chen J,Hui L,Ji H,Zhu X

The translation initiation factor 3f (eIF3f) exhibits a deubiquitinase activity regulating Notch activation.
PLoS biology8(11)e1000545(2010 Nov)
Moretti J,Chastagner P,Gastaldello S,Heuss SF,Dirac AM,Bernards R,Masucci MG,Isra?l A,Brou C

The translation regulatory subunit eIF3f controls the kinase-dependent mTOR signaling required for muscle differentiation and hypertrophy in mouse.
PloS one5(2)e8994(2010 Feb)
Csibi A,Cornille K,Leibovitch MP,Poupon A,Tintignac LA,Sanchez AM,Leibovitch SA

Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease.
Physiological genomics39(3)160-8(2009 Nov)
Tkatchenko TV,Moreno-Rodriguez RA,Conway SJ,Molkentin JD,Markwald RR,Tkatchenko AV

MAFbx/Atrogin-1 controls the activity of the initiation factor eIF3-f in skeletal muscle atrophy by targeting multiple C-terminal lysines.
The Journal of biological chemistry284(7)4413-21(2009 Feb)
Csibi A,Leibovitch MP,Cornille K,Tintignac LA,Leibovitch SA

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Eif3f cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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