Map3k20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map3k20
Entrez Gene ID	65964
Full Name	mitogen-activated protein kinase kinase kinase 20
Synonyms	AV006891,B230120H23Rik,HCCS-4,MLTK,MLTKalpha,MLTKbeta,Zak
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 20 Names mitogen-activated protein kinase kinase kinase 20 mitogen-activated protein kinase kinase kinase MLT MLK-like mitogen-activated protein triple kinase MLK-related kinase MRK human cervical cancer suppressor gene 4 protein leucine zipper- and sterile alpha motif kinase ZAK leucine zipper- and sterile alpha motif-containing kinase mixed lineage kinase-related kinase sterile alpha motif and leucine zipper containing kinase AZK
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499998.3	XP_006500061.1	mitogen-activated protein kinase kinase kinase 20 isoform X2
XM_006499998.2	XP_006500061.1	mitogen-activated protein kinase kinase kinase MLT isoform X2
XM_006499997.2	XP_006500060.1	mitogen-activated protein kinase kinase kinase MLT isoform X1
XM_030251937.1	XP_030107797.1	mitogen-activated protein kinase kinase kinase 20 isoform X3
NM_178084.4	NP_835185.2	mitogen-activated protein kinase kinase kinase 20 isoform 2
NM_001164791.1	NP_001158263.1	mitogen-activated protein kinase kinase kinase 20 isoform 3
XM_006499997.3	XP_006500060.1	mitogen-activated protein kinase kinase kinase 20 isoform X1
NM_023057.5	NP_075544.1	mitogen-activated protein kinase kinase kinase 20 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Zak	NP_075544.1
Rattus norvegicus (Norway rat)	Zak	XP_006234431.1
Xenopus tropicalis (tropical clawed frog)	LOC100495797	XP_002934652.1
Homo sapiens (human)	ZAK	NP_057737.2
Danio rerio (zebrafish)	si:dkey-15i8.3	XP_687660.2
Pan troglodytes (chimpanzee)	LOC459745	XP_003309476.1
Bos taurus (cattle)	ZAK	NP_001192422.1
Gallus gallus (chicken)	ZAK	XP_421996.4
	ZAK	XP_001086798.2
Canis lupus familiaris (dog)	ZAK	XP_535966.2

Gene Ontology
Bibliography

Related articles in PubMed

A Novel Zak Knockout Mouse with a Defective Ribotoxic Stress Response.
Jandhyala DM, Wong J, Mantis NJ, Magun BE, Leong JM, Thorpe CM
Toxins8(9)(2016 09)

Exome sequencing and CRISPR/Cas genome editing identify mutations of ZAK as a cause of limb defects in humans and mice.
Spielmann M, Kakar N, Tayebi N, Leettola C, N?rnberg G, Sowada N, Lupi??ez DG, Harabula I, Fl?ttmann R, Horn D, Chan WL, Wittler L, Yilmaz R, Altm?ller J, Thiele H, van Bokhoven H, Schwartz CE, N?rnberg P, Bowie JU, Ahmad J, Kubisch C, Mundlos S, Borck G
Genome research26(2)183-91(2016 Feb)

Searching for genomic region of high-fat diet-induced type 2 diabetes in mouse chromosome 2 by analysis of congenic strains.
Kobayashi M, Ohno T, Ihara K, Murai A, Kumazawa M, Hoshino H, Iwanaga K, Iwai H, Hamana Y, Ito M, Ohno K, Horio F
PloS one9(5)e96271(2014)

Identification of transcripts with enriched expression in the developing and adult pancreas.
Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, Helgason CD
Genome biology9(6)R99(2008)

Complete inhibition of anisomycin and UV radiation but not cytokine induced JNK and p38 activation by an aryl-substituted dihydropyrrolopyrazole quinoline and mixed lineage kinase 7 small interfering RNA.
Wang X, Mader MM, Toth JE, Yu X, Jin N, Campbell RM, Smallwood JK, Christe ME, Chatterjee A, Goodson T Jr, Vlahos CJ, Matter WF, Bloem LJ
The Journal of biological chemistry280(19)19298-305(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zak-/- mice demonstrate a distinct ribotoxic stress-associated phenotype in response to ricin.
Title: A Novel Zak Knockout Mouse with a Defective Ribotoxic Stress Response.

ZAK is a key player in mammalian limb patterning in humans and mice.
Title: Exome sequencing and CRISPR/Cas genome editing identify mutations of ZAK as a cause of limb defects in humans and mice.

Exome sequencing analysis between SM/J and A/J mice identified 4 genes (Itga6, Zak, Gpr155, and Mtx2) with nonsynonymous coding SNPs. These four genes might be candidate genes for type 2 diabetes caused by gene-gene interactions.
Title: Searching for genomic region of high-fat diet-induced type 2 diabetes in mouse chromosome 2 by analysis of congenic strains.

Mixed lineage kinase 7 (MLK7), a splice variant of the human ZAK gene (ortholog of mouse B230120H23Rik), encodes an isoform which is the MAPKKK required for modulation of the stress-activated MAPKs downstream of anisomycin and UV stimulation.
Title: Complete inhibition of anisomycin and UV radiation but not cytokine induced JNK and p38 activation by an aryl-substituted dihydropyrrolopyrazole quinoline and mixed lineage kinase 7 small interfering RNA.

Results show that mixed lineage kinase-like mitogen-activated protein triple kinase (MLTK_-alpha plays a key role in the UVB- and EGF-induced phosphorylation of histone H3 at Ser28.
Title: Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha.

The following Map3k20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map3k20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15355	NM_178084.4 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu16012	NM_001164791.1 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu16012	XM_030251937.1 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu30771	XM_006499998.3 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu15276	NM_023057.5 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu15276	XM_006499997.3 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu30771	XM_006499998.2	Mus musculus sterile alpha motif and leucine zipper containing kinase AZK (Zak), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu15276	XM_006499997.2	Mus musculus sterile alpha motif and leucine zipper containing kinase AZK (Zak), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map3k20 ( XM_006499998.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500061.1 Mus musculus sterile alpha motif and leucine zipper containing kinase AZK (Zak), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu30771
Clone ID Related Accession (Same CDS sequence)	XM_006499998.3 , XM_006499998.2
Accession Version	XM_006499998.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase MLT isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006499998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGAGC AGAAGCTGAC GGAGCAATCC AACACCCCGC TGCTGCCTTC CTTTGAGATT 
GGTGCGTGGA CTGAAGACGA TGTGTATTTT TGGGTTCAGC AGCTCGTCAG AAAAGGCGAG 
TCTTCAGTAG AGATGAGTGG ATACGCAAGT TTGTTTAAGG AGAACAACAT CACAGGCAAG 
CGGTTGCTGC TTCTGGAAGA GGAAGATCTG AAAGACATGG GCATCGTCTC CAAGGGACAC 
ATCATTCACT TTAAGTCAGC TATTGAGAAG CTAACCCACG ATTATCTGAA CCTGTTTCAC 
TTCCCACCAC TGATTAAGGA TTCAGGAGGG GAACCTGAAG AAAATGAGGA AAAAATAGTG 
AACCTTGAAC TTGTTTTTGG TTTTCACTTG AAGCCAGGAA CTGGCCCACA GGATTGCAAA 
TGGAAAATGT ATATGGAGAT GGATGGGGAC GAAGTTGCAA TCACGTACAT AAAAGATGTG 
ACTTTCAACA CGAGCCTCCC TGATGCAGAG ATTTTAAAGA TGACAAAGCC ACCATTTGTA 
ATGGAGAAGT GGATCGTGGG AATAGCGGAG GATCAGACTG TGGAGTGCAC GGTCACCTAT 
GAGAATGATG TCAGAACACC AAAACTCACT AAGCACGTGC ACTCCATACA GTGGGACAGA 
ACGAAGCCTC AGGATGAGGT GAAGGCGGTC CAGCTTGCCA TTCAGACTCT GTTCTCCAGC 
TCAGAGGGCA ACCCTGGCAG CAGATCCGAC TCCAGTGCGG ACTGCCAATG GTTAGACACT 
CTGCGCATGC GGCAGATTGC ATCCCACACT TCTCTACAGC GTTCCCAAAG CAACCCTATC 
TTGGGGTCTC CTTTCTTCCC TTACTTTGCC AACCAGGATT CCTATGCTGC TGCGGTGAGG 
AGGACCCAGA CTCCGGTGAA GTACCAGCAG ATTACACCAA GCATCAACCC TTCCCGGAGC 
TCCTCCCCCA CGCAGTATGG GCTGTCCAGA AACTTCTCCT CCCTGAACCT CAGCTCCAGG 
GACAGCGGCT TCTCCAGCCT CAATGACAGC TCCTCCGAGA GGGGCCGCTA CTCAGACCGA 
AGCAGAAACA AGTACTACCG TGGTAGTGTG TCCCTCAATT CTTCCCCGAA AGGAAGATAT 
GGTGGGAAAA GTCAGCATTC CACACCTTCA AGAGAAAGAT ATTCTGGAAA GTTCTACAGG 
CTGCCCCAGT CAGCGCTGAA CACTCATCAG TCCCCTGACT TCAAGAGGAG CCCAAATGAC 
CATGACCGCC GTGTGCCCAG GACCATACCC GGGATGCCTC TGCACCCTGA GACTGCCTCC 
AAAGCAGGGG AAGAGGAGAG CAGGGTGAGC GAAGGGGGCT GGACTAAAGT GGAATATCGG 
AAGAAGACAC ACAGGCAACT TTCGGCCAAG ACTAGCAAGG AGAGAACCCG TGGCAACTAC 
CGTGGGCGGC GGAATTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500061.1
CDS	968..2428
Translation	MWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGESSVEMSGYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYLNLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEVAITYIKDVTFNTSLPDAEILKMTKPPFVMEKWIVGIAEDQTVECTVTYENDVRTPKLTKHVHSIQWDRTKPQDEVKAVQLAIQTLFSSSEGNPGSRSDSSADCQWLDTLRMRQIASHTSLQRSQSNPILGSPFFPYFANQDSYAAAVRRTQTPVKYQQITPSINPSRSSSPTQYGLSRNFSSLNLSSRDSGFSSLNDSSSERGRYSDRSRNKYYRGSVSLNSSPKGRYGGKSQHSTPSRERYSGKFYRLPQSALNTHQSPDFKRSPNDHDRRVPRTIPGMPLHPETASKAGEEESRVSEGGWTKVEYRKKTHRQLSAKTSKERTRGNYRGRRNF

Target ORF information:

RefSeq Version	XM_006499998.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sterile alpha motif and leucine zipper containing kinase AZK (Zak), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499998.2

ORF Insert Sequence:

ATGTGGGAGC AGAAGCTGAC GGAGCAATCC AACACCCCGC TGCTGCCTTC CTTTGAGATT 
GGTGCGTGGA CTGAAGACGA TGTGTATTTT TGGGTTCAGC AGCTCGTCAG AAAAGGCGAG 
TCTTCAGTAG AGATGAGTGG ATACGCAAGT TTGTTTAAGG AGAACAACAT CACAGGCAAG 
CGGTTGCTGC TTCTGGAAGA GGAAGATCTG AAAGACATGG GCATCGTCTC CAAGGGACAC 
ATCATTCACT TTAAGTCAGC TATTGAGAAG CTAACCCACG ATTATCTGAA CCTGTTTCAC 
TTCCCACCAC TGATTAAGGA TTCAGGAGGG GAACCTGAAG AAAATGAGGA AAAAATAGTG 
AACCTTGAAC TTGTTTTTGG TTTTCACTTG AAGCCAGGAA CTGGCCCACA GGATTGCAAA 
TGGAAAATGT ATATGGAGAT GGATGGGGAC GAAGTTGCAA TCACGTACAT AAAAGATGTG 
ACTTTCAACA CGAGCCTCCC TGATGCAGAG ATTTTAAAGA TGACAAAGCC ACCATTTGTA 
ATGGAGAAGT GGATCGTGGG AATAGCGGAG GATCAGACTG TGGAGTGCAC GGTCACCTAT 
GAGAATGATG TCAGAACACC AAAACTCACT AAGCACGTGC ACTCCATACA GTGGGACAGA 
ACGAAGCCTC AGGATGAGGT GAAGGCGGTC CAGCTTGCCA TTCAGACTCT GTTCTCCAGC 
TCAGAGGGCA ACCCTGGCAG CAGATCCGAC TCCAGTGCGG ACTGCCAATG GTTAGACACT 
CTGCGCATGC GGCAGATTGC ATCCCACACT TCTCTACAGC GTTCCCAAAG CAACCCTATC 
TTGGGGTCTC CTTTCTTCCC TTACTTTGCC AACCAGGATT CCTATGCTGC TGCGGTGAGG 
AGGACCCAGA CTCCGGTGAA GTACCAGCAG ATTACACCAA GCATCAACCC TTCCCGGAGC 
TCCTCCCCCA CGCAGTATGG GCTGTCCAGA AACTTCTCCT CCCTGAACCT CAGCTCCAGG 
GACAGCGGCT TCTCCAGCCT CAATGACAGC TCCTCCGAGA GGGGCCGCTA CTCAGACCGA 
AGCAGAAACA AGTACTACCG TGGTAGTGTG TCCCTCAATT CTTCCCCGAA AGGAAGATAT 
GGTGGGAAAA GTCAGCATTC CACACCTTCA AGAGAAAGAT ATTCTGGAAA GTTCTACAGG 
CTGCCCCAGT CAGCGCTGAA CACTCATCAG TCCCCTGACT TCAAGAGGAG CCCAAATGAC 
CATGACCGCC GTGTGCCCAG GACCATACCC GGGATGCCTC TGCACCCTGA GACTGCCTCC 
AAAGCAGGGG AAGAGGAGAG CAGGGTGAGC GAAGGGGGCT GGACTAAAGT GGAATATCGG 
AAGAAGACAC ACAGGCAACT TTCGGCCAAG ACTAGCAAGG AGAGAACCCG TGGCAACTAC 
CGTGGGCGGC GGAATTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k20 ( NM_178084.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_835185.2 Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15355
Clone ID Related Accession (Same CDS sequence)	NM_178084.4
Accession Version	NM_178084.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 20 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AV470816.1, AK045444.1 and AK132186.1. On Sep 18, 2009 this sequence version replaced NM_178084.3. Transcript Variant: This variant (2) lacks multiple 3' exon but has an alternate 3' segment, as compared to variant 1. The resulting isoform (2) is much shorter and has a distinct C-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK045444.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGGGTAGC TCCGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835185.2
CDS	190..1059
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRWVAPA

Target ORF information:

RefSeq Version	NM_178084.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178084.4

ORF Insert Sequence:

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGGGTAGC TCCGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k20 ( NM_001164791.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001158263.1 Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu16012
Clone ID Related Accession (Same CDS sequence)	XM_030251937.1 , NM_001164791.1
Accession Version	NM_001164791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 20 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AV470816.1, AK162819.1, AL928699.9 and AW049206.1. Transcript Variant: This variant (3) lacks several 3' exon but has an alternate 3' exon, as compared to variant 1. The resulting isoform (3) is shorter and has a distinct C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK162819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTT CTCTTGCCTC TCTCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGCCAGATC 
ACTGCAGCAA GTAACGGGGA GGGCCATGGC ATGAACCCAG GCCTGCAGGC CATGATGCTC 
ATGGGCTTTG GGGATGTCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCA TCCAAAACCA CATGTAAGAG GAGAGGGAAG 
AAAGTCAACA TGGCCCTGGG GTTCAGTGAC TTTGACCTGT CAGAAGGTGA CGATGATGAC 
CATGATGGTG ACGATGCTGA GAATGATGTG GATAATAGTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158263.1
CDS	190..1554
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLSARMSEESYFESKTEESNSAEMSCQITAASNGEGHGMNPGLQAMMLMGFGDVFSMNKAGAVLHSGMQINMQAKQNSSKTTCKRRGKKVNMALGFSDFDLSEGDDDDHDGDDAENDVDNSE

Target ORF information:

RefSeq Version	NM_001164791.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164791.1

ORF Insert Sequence:

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTT CTCTTGCCTC TCTCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGCCAGATC 
ACTGCAGCAA GTAACGGGGA GGGCCATGGC ATGAACCCAG GCCTGCAGGC CATGATGCTC 
ATGGGCTTTG GGGATGTCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCA TCCAAAACCA CATGTAAGAG GAGAGGGAAG 
AAAGTCAACA TGGCCCTGGG GTTCAGTGAC TTTGACCTGT CAGAAGGTGA CGATGATGAC 
CATGATGGTG ACGATGCTGA GAATGATGTG GATAATAGTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k20 ( XM_030251937.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107797.1 Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X3, mRNA.

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CloneID	OMu16012
Clone ID Related Accession (Same CDS sequence)	XM_030251937.1 , NM_001164791.1
Accession Version	XM_030251937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTT CTCTTGCCTC TCTCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGCCAGATC 
ACTGCAGCAA GTAACGGGGA GGGCCATGGC ATGAACCCAG GCCTGCAGGC CATGATGCTC 
ATGGGCTTTG GGGATGTCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCA TCCAAAACCA CATGTAAGAG GAGAGGGAAG 
AAAGTCAACA TGGCCCTGGG GTTCAGTGAC TTTGACCTGT CAGAAGGTGA CGATGATGAC 
CATGATGGTG ACGATGCTGA GAATGATGTG GATAATAGTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107797.1
CDS	147..1511
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLSARMSEESYFESKTEESNSAEMSCQITAASNGEGHGMNPGLQAMMLMGFGDVFSMNKAGAVLHSGMQINMQAKQNSSKTTCKRRGKKVNMALGFSDFDLSEGDDDDHDGDDAENDVDNSE

Target ORF information:

RefSeq Version	XM_030251937.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251937.1

ORF Insert Sequence:

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTT CTCTTGCCTC TCTCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGCCAGATC 
ACTGCAGCAA GTAACGGGGA GGGCCATGGC ATGAACCCAG GCCTGCAGGC CATGATGCTC 
ATGGGCTTTG GGGATGTCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCA TCCAAAACCA CATGTAAGAG GAGAGGGAAG 
AAAGTCAACA TGGCCCTGGG GTTCAGTGAC TTTGACCTGT CAGAAGGTGA CGATGATGAC 
CATGATGGTG ACGATGCTGA GAATGATGTG GATAATAGTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k20 ( XM_006499998.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500061.1 Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X2, mRNA.

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CloneID	OMu30771
Clone ID Related Accession (Same CDS sequence)	XM_006499998.3 , XM_006499998.2
Accession Version	XM_006499998.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499998.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGAGC AGAAGCTGAC GGAGCAATCC AACACCCCGC TGCTGCCTTC CTTTGAGATT 
GGTGCGTGGA CTGAAGACGA TGTGTATTTT TGGGTTCAGC AGCTCGTCAG AAAAGGCGAG 
TCTTCAGTAG AGATGAGTGG ATACGCAAGT TTGTTTAAGG AGAACAACAT CACAGGCAAG 
CGGTTGCTGC TTCTGGAAGA GGAAGATCTG AAAGACATGG GCATCGTCTC CAAGGGACAC 
ATCATTCACT TTAAGTCAGC TATTGAGAAG CTAACCCACG ATTATCTGAA CCTGTTTCAC 
TTCCCACCAC TGATTAAGGA TTCAGGAGGG GAACCTGAAG AAAATGAGGA AAAAATAGTG 
AACCTTGAAC TTGTTTTTGG TTTTCACTTG AAGCCAGGAA CTGGCCCACA GGATTGCAAA 
TGGAAAATGT ATATGGAGAT GGATGGGGAC GAAGTTGCAA TCACGTACAT AAAAGATGTG 
ACTTTCAACA CGAGCCTCCC TGATGCAGAG ATTTTAAAGA TGACAAAGCC ACCATTTGTA 
ATGGAGAAGT GGATCGTGGG AATAGCGGAG GATCAGACTG TGGAGTGCAC GGTCACCTAT 
GAGAATGATG TCAGAACACC AAAACTCACT AAGCACGTGC ACTCCATACA GTGGGACAGA 
ACGAAGCCTC AGGATGAGGT GAAGGCGGTC CAGCTTGCCA TTCAGACTCT GTTCTCCAGC 
TCAGAGGGCA ACCCTGGCAG CAGATCCGAC TCCAGTGCGG ACTGCCAATG GTTAGACACT 
CTGCGCATGC GGCAGATTGC ATCCCACACT TCTCTACAGC GTTCCCAAAG CAACCCTATC 
TTGGGGTCTC CTTTCTTCCC TTACTTTGCC AACCAGGATT CCTATGCTGC TGCGGTGAGG 
AGGACCCAGA CTCCGGTGAA GTACCAGCAG ATTACACCAA GCATCAACCC TTCCCGGAGC 
TCCTCCCCCA CGCAGTATGG GCTGTCCAGA AACTTCTCCT CCCTGAACCT CAGCTCCAGG 
GACAGCGGCT TCTCCAGCCT CAATGACAGC TCCTCCGAGA GGGGCCGCTA CTCAGACCGA 
AGCAGAAACA AGTACTACCG TGGTAGTGTG TCCCTCAATT CTTCCCCGAA AGGAAGATAT 
GGTGGGAAAA GTCAGCATTC CACACCTTCA AGAGAAAGAT ATTCTGGAAA GTTCTACAGG 
CTGCCCCAGT CAGCGCTGAA CACTCATCAG TCCCCTGACT TCAAGAGGAG CCCAAATGAC 
CATGACCGCC GTGTGCCCAG GACCATACCC GGGATGCCTC TGCACCCTGA GACTGCCTCC 
AAAGCAGGGG AAGAGGAGAG CAGGGTGAGC GAAGGGGGCT GGACTAAAGT GGAATATCGG 
AAGAAGACAC ACAGGCAACT TTCGGCCAAG ACTAGCAAGG AGAGAACCCG TGGCAACTAC 
CGTGGGCGGC GGAATTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500061.1
CDS	964..2424
Translation	MWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGESSVEMSGYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYLNLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEVAITYIKDVTFNTSLPDAEILKMTKPPFVMEKWIVGIAEDQTVECTVTYENDVRTPKLTKHVHSIQWDRTKPQDEVKAVQLAIQTLFSSSEGNPGSRSDSSADCQWLDTLRMRQIASHTSLQRSQSNPILGSPFFPYFANQDSYAAAVRRTQTPVKYQQITPSINPSRSSSPTQYGLSRNFSSLNLSSRDSGFSSLNDSSSERGRYSDRSRNKYYRGSVSLNSSPKGRYGGKSQHSTPSRERYSGKFYRLPQSALNTHQSPDFKRSPNDHDRRVPRTIPGMPLHPETASKAGEEESRVSEGGWTKVEYRKKTHRQLSAKTSKERTRGNYRGRRNF

Target ORF information:

RefSeq Version	XM_006499998.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499998.3

ORF Insert Sequence:

ATGTGGGAGC AGAAGCTGAC GGAGCAATCC AACACCCCGC TGCTGCCTTC CTTTGAGATT 
GGTGCGTGGA CTGAAGACGA TGTGTATTTT TGGGTTCAGC AGCTCGTCAG AAAAGGCGAG 
TCTTCAGTAG AGATGAGTGG ATACGCAAGT TTGTTTAAGG AGAACAACAT CACAGGCAAG 
CGGTTGCTGC TTCTGGAAGA GGAAGATCTG AAAGACATGG GCATCGTCTC CAAGGGACAC 
ATCATTCACT TTAAGTCAGC TATTGAGAAG CTAACCCACG ATTATCTGAA CCTGTTTCAC 
TTCCCACCAC TGATTAAGGA TTCAGGAGGG GAACCTGAAG AAAATGAGGA AAAAATAGTG 
AACCTTGAAC TTGTTTTTGG TTTTCACTTG AAGCCAGGAA CTGGCCCACA GGATTGCAAA 
TGGAAAATGT ATATGGAGAT GGATGGGGAC GAAGTTGCAA TCACGTACAT AAAAGATGTG 
ACTTTCAACA CGAGCCTCCC TGATGCAGAG ATTTTAAAGA TGACAAAGCC ACCATTTGTA 
ATGGAGAAGT GGATCGTGGG AATAGCGGAG GATCAGACTG TGGAGTGCAC GGTCACCTAT 
GAGAATGATG TCAGAACACC AAAACTCACT AAGCACGTGC ACTCCATACA GTGGGACAGA 
ACGAAGCCTC AGGATGAGGT GAAGGCGGTC CAGCTTGCCA TTCAGACTCT GTTCTCCAGC 
TCAGAGGGCA ACCCTGGCAG CAGATCCGAC TCCAGTGCGG ACTGCCAATG GTTAGACACT 
CTGCGCATGC GGCAGATTGC ATCCCACACT TCTCTACAGC GTTCCCAAAG CAACCCTATC 
TTGGGGTCTC CTTTCTTCCC TTACTTTGCC AACCAGGATT CCTATGCTGC TGCGGTGAGG 
AGGACCCAGA CTCCGGTGAA GTACCAGCAG ATTACACCAA GCATCAACCC TTCCCGGAGC 
TCCTCCCCCA CGCAGTATGG GCTGTCCAGA AACTTCTCCT CCCTGAACCT CAGCTCCAGG 
GACAGCGGCT TCTCCAGCCT CAATGACAGC TCCTCCGAGA GGGGCCGCTA CTCAGACCGA 
AGCAGAAACA AGTACTACCG TGGTAGTGTG TCCCTCAATT CTTCCCCGAA AGGAAGATAT 
GGTGGGAAAA GTCAGCATTC CACACCTTCA AGAGAAAGAT ATTCTGGAAA GTTCTACAGG 
CTGCCCCAGT CAGCGCTGAA CACTCATCAG TCCCCTGACT TCAAGAGGAG CCCAAATGAC 
CATGACCGCC GTGTGCCCAG GACCATACCC GGGATGCCTC TGCACCCTGA GACTGCCTCC 
AAAGCAGGGG AAGAGGAGAG CAGGGTGAGC GAAGGGGGCT GGACTAAAGT GGAATATCGG 
AAGAAGACAC ACAGGCAACT TTCGGCCAAG ACTAGCAAGG AGAGAACCCG TGGCAACTAC 
CGTGGGCGGC GGAATTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k20 ( NM_023057.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075544.1 Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 1, mRNA.

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CloneID	OMu15276
Clone ID Related Accession (Same CDS sequence)	XM_006499997.2 , XM_006499997.3 , NM_023057.5
Accession Version	NM_023057.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 20 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132186.1 and AB049731.1. On Sep 18, 2009 this sequence version replaced NM_023057.4. Transcript Variant: This variant (1) is the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK132186.1, BC023718.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCGTGGACT 
GAAGACGATG TGTATTTTTG GGTTCAGCAG CTCGTCAGAA AAGGCGAGTC TTCAGTAGAG 
ATGAGTGGAT ACGCAAGTTT GTTTAAGGAG AACAACATCA CAGGCAAGCG GTTGCTGCTT 
CTGGAAGAGG AAGATCTGAA AGACATGGGC ATCGTCTCCA AGGGACACAT CATTCACTTT 
AAGTCAGCTA TTGAGAAGCT AACCCACGAT TATCTGAACC TGTTTCACTT CCCACCACTG 
ATTAAGGATT CAGGAGGGGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA GCCAGGAACT GGCCCACAGG ATTGCAAATG GAAAATGTAT 
ATGGAGATGG ATGGGGACGA AGTTGCAATC ACGTACATAA AAGATGTGAC TTTCAACACG 
AGCCTCCCTG ATGCAGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATCGTGGGAA TAGCGGAGGA TCAGACTGTG GAGTGCACGG TCACCTATGA GAATGATGTC 
AGAACACCAA AACTCACTAA GCACGTGCAC TCCATACAGT GGGACAGAAC GAAGCCTCAG 
GATGAGGTGA AGGCGGTCCA GCTTGCCATT CAGACTCTGT TCTCCAGCTC AGAGGGCAAC 
CCTGGCAGCA GATCCGACTC CAGTGCGGAC TGCCAATGGT TAGACACTCT GCGCATGCGG 
CAGATTGCAT CCCACACTTC TCTACAGCGT TCCCAAAGCA ACCCTATCTT GGGGTCTCCT 
TTCTTCCCTT ACTTTGCCAA CCAGGATTCC TATGCTGCTG CGGTGAGGAG GACCCAGACT 
CCGGTGAAGT ACCAGCAGAT TACACCAAGC ATCAACCCTT CCCGGAGCTC CTCCCCCACG 
CAGTATGGGC TGTCCAGAAA CTTCTCCTCC CTGAACCTCA GCTCCAGGGA CAGCGGCTTC 
TCCAGCCTCA ATGACAGCTC CTCCGAGAGG GGCCGCTACT CAGACCGAAG CAGAAACAAG 
TACTACCGTG GTAGTGTGTC CCTCAATTCT TCCCCGAAAG GAAGATATGG TGGGAAAAGT 
CAGCATTCCA CACCTTCAAG AGAAAGATAT TCTGGAAAGT TCTACAGGCT GCCCCAGTCA 
GCGCTGAACA CTCATCAGTC CCCTGACTTC AAGAGGAGCC CAAATGACCA TGACCGCCGT 
GTGCCCAGGA CCATACCCGG GATGCCTCTG CACCCTGAGA CTGCCTCCAA AGCAGGGGAA 
GAGGAGAGCA GGGTGAGCGA AGGGGGCTGG ACTAAAGTGG AATATCGGAA GAAGACACAC 
AGGCAACTTT CGGCCAAGAC TAGCAAGGAG AGAACCCGTG GCAACTACCG TGGGCGGCGG 
AATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075544.1
CDS	254..2662
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGESSVEMSGYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYLNLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEVAITYIKDVTFNTSLPDAEILKMTKPPFVMEKWIVGIAEDQTVECTVTYENDVRTPKLTKHVHSIQWDRTKPQDEVKAVQLAIQTLFSSSEGNPGSRSDSSADCQWLDTLRMRQIASHTSLQRSQSNPILGSPFFPYFANQDSYAAAVRRTQTPVKYQQITPSINPSRSSSPTQYGLSRNFSSLNLSSRDSGFSSLNDSSSERGRYSDRSRNKYYRGSVSLNSSPKGRYGGKSQHSTPSRERYSGKFYRLPQSALNTHQSPDFKRSPNDHDRRVPRTIPGMPLHPETASKAGEEESRVSEGGWTKVEYRKKTHRQLSAKTSKERTRGNYRGRRNF

Target ORF information:

RefSeq Version	NM_023057.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023057.5

ORF Insert Sequence:

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCGTGGACT 
GAAGACGATG TGTATTTTTG GGTTCAGCAG CTCGTCAGAA AAGGCGAGTC TTCAGTAGAG 
ATGAGTGGAT ACGCAAGTTT GTTTAAGGAG AACAACATCA CAGGCAAGCG GTTGCTGCTT 
CTGGAAGAGG AAGATCTGAA AGACATGGGC ATCGTCTCCA AGGGACACAT CATTCACTTT 
AAGTCAGCTA TTGAGAAGCT AACCCACGAT TATCTGAACC TGTTTCACTT CCCACCACTG 
ATTAAGGATT CAGGAGGGGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA GCCAGGAACT GGCCCACAGG ATTGCAAATG GAAAATGTAT 
ATGGAGATGG ATGGGGACGA AGTTGCAATC ACGTACATAA AAGATGTGAC TTTCAACACG 
AGCCTCCCTG ATGCAGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATCGTGGGAA TAGCGGAGGA TCAGACTGTG GAGTGCACGG TCACCTATGA GAATGATGTC 
AGAACACCAA AACTCACTAA GCACGTGCAC TCCATACAGT GGGACAGAAC GAAGCCTCAG 
GATGAGGTGA AGGCGGTCCA GCTTGCCATT CAGACTCTGT TCTCCAGCTC AGAGGGCAAC 
CCTGGCAGCA GATCCGACTC CAGTGCGGAC TGCCAATGGT TAGACACTCT GCGCATGCGG 
CAGATTGCAT CCCACACTTC TCTACAGCGT TCCCAAAGCA ACCCTATCTT GGGGTCTCCT 
TTCTTCCCTT ACTTTGCCAA CCAGGATTCC TATGCTGCTG CGGTGAGGAG GACCCAGACT 
CCGGTGAAGT ACCAGCAGAT TACACCAAGC ATCAACCCTT CCCGGAGCTC CTCCCCCACG 
CAGTATGGGC TGTCCAGAAA CTTCTCCTCC CTGAACCTCA GCTCCAGGGA CAGCGGCTTC 
TCCAGCCTCA ATGACAGCTC CTCCGAGAGG GGCCGCTACT CAGACCGAAG CAGAAACAAG 
TACTACCGTG GTAGTGTGTC CCTCAATTCT TCCCCGAAAG GAAGATATGG TGGGAAAAGT 
CAGCATTCCA CACCTTCAAG AGAAAGATAT TCTGGAAAGT TCTACAGGCT GCCCCAGTCA 
GCGCTGAACA CTCATCAGTC CCCTGACTTC AAGAGGAGCC CAAATGACCA TGACCGCCGT 
GTGCCCAGGA CCATACCCGG GATGCCTCTG CACCCTGAGA CTGCCTCCAA AGCAGGGGAA 
GAGGAGAGCA GGGTGAGCGA AGGGGGCTGG ACTAAAGTGG AATATCGGAA GAAGACACAC 
AGGCAACTTT CGGCCAAGAC TAGCAAGGAG AGAACCCGTG GCAACTACCG TGGGCGGCGG 
AATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k20 ( XM_006499997.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500060.1 Mus musculus sterile alpha motif and leucine zipper containing kinase AZK (Zak), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15276
Clone ID Related Accession (Same CDS sequence)	XM_006499997.2 , XM_006499997.3 , NM_023057.5
Accession Version	XM_006499997.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase MLT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006499997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCGTGGACT 
GAAGACGATG TGTATTTTTG GGTTCAGCAG CTCGTCAGAA AAGGCGAGTC TTCAGTAGAG 
ATGAGTGGAT ACGCAAGTTT GTTTAAGGAG AACAACATCA CAGGCAAGCG GTTGCTGCTT 
CTGGAAGAGG AAGATCTGAA AGACATGGGC ATCGTCTCCA AGGGACACAT CATTCACTTT 
AAGTCAGCTA TTGAGAAGCT AACCCACGAT TATCTGAACC TGTTTCACTT CCCACCACTG 
ATTAAGGATT CAGGAGGGGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA GCCAGGAACT GGCCCACAGG ATTGCAAATG GAAAATGTAT 
ATGGAGATGG ATGGGGACGA AGTTGCAATC ACGTACATAA AAGATGTGAC TTTCAACACG 
AGCCTCCCTG ATGCAGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATCGTGGGAA TAGCGGAGGA TCAGACTGTG GAGTGCACGG TCACCTATGA GAATGATGTC 
AGAACACCAA AACTCACTAA GCACGTGCAC TCCATACAGT GGGACAGAAC GAAGCCTCAG 
GATGAGGTGA AGGCGGTCCA GCTTGCCATT CAGACTCTGT TCTCCAGCTC AGAGGGCAAC 
CCTGGCAGCA GATCCGACTC CAGTGCGGAC TGCCAATGGT TAGACACTCT GCGCATGCGG 
CAGATTGCAT CCCACACTTC TCTACAGCGT TCCCAAAGCA ACCCTATCTT GGGGTCTCCT 
TTCTTCCCTT ACTTTGCCAA CCAGGATTCC TATGCTGCTG CGGTGAGGAG GACCCAGACT 
CCGGTGAAGT ACCAGCAGAT TACACCAAGC ATCAACCCTT CCCGGAGCTC CTCCCCCACG 
CAGTATGGGC TGTCCAGAAA CTTCTCCTCC CTGAACCTCA GCTCCAGGGA CAGCGGCTTC 
TCCAGCCTCA ATGACAGCTC CTCCGAGAGG GGCCGCTACT CAGACCGAAG CAGAAACAAG 
TACTACCGTG GTAGTGTGTC CCTCAATTCT TCCCCGAAAG GAAGATATGG TGGGAAAAGT 
CAGCATTCCA CACCTTCAAG AGAAAGATAT TCTGGAAAGT TCTACAGGCT GCCCCAGTCA 
GCGCTGAACA CTCATCAGTC CCCTGACTTC AAGAGGAGCC CAAATGACCA TGACCGCCGT 
GTGCCCAGGA CCATACCCGG GATGCCTCTG CACCCTGAGA CTGCCTCCAA AGCAGGGGAA 
GAGGAGAGCA GGGTGAGCGA AGGGGGCTGG ACTAAAGTGG AATATCGGAA GAAGACACAC 
AGGCAACTTT CGGCCAAGAC TAGCAAGGAG AGAACCCGTG GCAACTACCG TGGGCGGCGG 
AATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500060.1
CDS	430..2838
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGESSVEMSGYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYLNLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEVAITYIKDVTFNTSLPDAEILKMTKPPFVMEKWIVGIAEDQTVECTVTYENDVRTPKLTKHVHSIQWDRTKPQDEVKAVQLAIQTLFSSSEGNPGSRSDSSADCQWLDTLRMRQIASHTSLQRSQSNPILGSPFFPYFANQDSYAAAVRRTQTPVKYQQITPSINPSRSSSPTQYGLSRNFSSLNLSSRDSGFSSLNDSSSERGRYSDRSRNKYYRGSVSLNSSPKGRYGGKSQHSTPSRERYSGKFYRLPQSALNTHQSPDFKRSPNDHDRRVPRTIPGMPLHPETASKAGEEESRVSEGGWTKVEYRKKTHRQLSAKTSKERTRGNYRGRRNF

Target ORF information:

RefSeq Version	XM_006499997.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sterile alpha motif and leucine zipper containing kinase AZK (Zak), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499997.2

ORF Insert Sequence:

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCGTGGACT 
GAAGACGATG TGTATTTTTG GGTTCAGCAG CTCGTCAGAA AAGGCGAGTC TTCAGTAGAG 
ATGAGTGGAT ACGCAAGTTT GTTTAAGGAG AACAACATCA CAGGCAAGCG GTTGCTGCTT 
CTGGAAGAGG AAGATCTGAA AGACATGGGC ATCGTCTCCA AGGGACACAT CATTCACTTT 
AAGTCAGCTA TTGAGAAGCT AACCCACGAT TATCTGAACC TGTTTCACTT CCCACCACTG 
ATTAAGGATT CAGGAGGGGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA GCCAGGAACT GGCCCACAGG ATTGCAAATG GAAAATGTAT 
ATGGAGATGG ATGGGGACGA AGTTGCAATC ACGTACATAA AAGATGTGAC TTTCAACACG 
AGCCTCCCTG ATGCAGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATCGTGGGAA TAGCGGAGGA TCAGACTGTG GAGTGCACGG TCACCTATGA GAATGATGTC 
AGAACACCAA AACTCACTAA GCACGTGCAC TCCATACAGT GGGACAGAAC GAAGCCTCAG 
GATGAGGTGA AGGCGGTCCA GCTTGCCATT CAGACTCTGT TCTCCAGCTC AGAGGGCAAC 
CCTGGCAGCA GATCCGACTC CAGTGCGGAC TGCCAATGGT TAGACACTCT GCGCATGCGG 
CAGATTGCAT CCCACACTTC TCTACAGCGT TCCCAAAGCA ACCCTATCTT GGGGTCTCCT 
TTCTTCCCTT ACTTTGCCAA CCAGGATTCC TATGCTGCTG CGGTGAGGAG GACCCAGACT 
CCGGTGAAGT ACCAGCAGAT TACACCAAGC ATCAACCCTT CCCGGAGCTC CTCCCCCACG 
CAGTATGGGC TGTCCAGAAA CTTCTCCTCC CTGAACCTCA GCTCCAGGGA CAGCGGCTTC 
TCCAGCCTCA ATGACAGCTC CTCCGAGAGG GGCCGCTACT CAGACCGAAG CAGAAACAAG 
TACTACCGTG GTAGTGTGTC CCTCAATTCT TCCCCGAAAG GAAGATATGG TGGGAAAAGT 
CAGCATTCCA CACCTTCAAG AGAAAGATAT TCTGGAAAGT TCTACAGGCT GCCCCAGTCA 
GCGCTGAACA CTCATCAGTC CCCTGACTTC AAGAGGAGCC CAAATGACCA TGACCGCCGT 
GTGCCCAGGA CCATACCCGG GATGCCTCTG CACCCTGAGA CTGCCTCCAA AGCAGGGGAA 
GAGGAGAGCA GGGTGAGCGA AGGGGGCTGG ACTAAAGTGG AATATCGGAA GAAGACACAC 
AGGCAACTTT CGGCCAAGAC TAGCAAGGAG AGAACCCGTG GCAACTACCG TGGGCGGCGG 
AATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k20 ( XM_006499997.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500060.1 Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15276
Clone ID Related Accession (Same CDS sequence)	XM_006499997.2 , XM_006499997.3 , NM_023057.5
Accession Version	XM_006499997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCGTGGACT 
GAAGACGATG TGTATTTTTG GGTTCAGCAG CTCGTCAGAA AAGGCGAGTC TTCAGTAGAG 
ATGAGTGGAT ACGCAAGTTT GTTTAAGGAG AACAACATCA CAGGCAAGCG GTTGCTGCTT 
CTGGAAGAGG AAGATCTGAA AGACATGGGC ATCGTCTCCA AGGGACACAT CATTCACTTT 
AAGTCAGCTA TTGAGAAGCT AACCCACGAT TATCTGAACC TGTTTCACTT CCCACCACTG 
ATTAAGGATT CAGGAGGGGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA GCCAGGAACT GGCCCACAGG ATTGCAAATG GAAAATGTAT 
ATGGAGATGG ATGGGGACGA AGTTGCAATC ACGTACATAA AAGATGTGAC TTTCAACACG 
AGCCTCCCTG ATGCAGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATCGTGGGAA TAGCGGAGGA TCAGACTGTG GAGTGCACGG TCACCTATGA GAATGATGTC 
AGAACACCAA AACTCACTAA GCACGTGCAC TCCATACAGT GGGACAGAAC GAAGCCTCAG 
GATGAGGTGA AGGCGGTCCA GCTTGCCATT CAGACTCTGT TCTCCAGCTC AGAGGGCAAC 
CCTGGCAGCA GATCCGACTC CAGTGCGGAC TGCCAATGGT TAGACACTCT GCGCATGCGG 
CAGATTGCAT CCCACACTTC TCTACAGCGT TCCCAAAGCA ACCCTATCTT GGGGTCTCCT 
TTCTTCCCTT ACTTTGCCAA CCAGGATTCC TATGCTGCTG CGGTGAGGAG GACCCAGACT 
CCGGTGAAGT ACCAGCAGAT TACACCAAGC ATCAACCCTT CCCGGAGCTC CTCCCCCACG 
CAGTATGGGC TGTCCAGAAA CTTCTCCTCC CTGAACCTCA GCTCCAGGGA CAGCGGCTTC 
TCCAGCCTCA ATGACAGCTC CTCCGAGAGG GGCCGCTACT CAGACCGAAG CAGAAACAAG 
TACTACCGTG GTAGTGTGTC CCTCAATTCT TCCCCGAAAG GAAGATATGG TGGGAAAAGT 
CAGCATTCCA CACCTTCAAG AGAAAGATAT TCTGGAAAGT TCTACAGGCT GCCCCAGTCA 
GCGCTGAACA CTCATCAGTC CCCTGACTTC AAGAGGAGCC CAAATGACCA TGACCGCCGT 
GTGCCCAGGA CCATACCCGG GATGCCTCTG CACCCTGAGA CTGCCTCCAA AGCAGGGGAA 
GAGGAGAGCA GGGTGAGCGA AGGGGGCTGG ACTAAAGTGG AATATCGGAA GAAGACACAC 
AGGCAACTTT CGGCCAAGAC TAGCAAGGAG AGAACCCGTG GCAACTACCG TGGGCGGCGG 
AATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500060.1
CDS	424..2832
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGESSVEMSGYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYLNLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEVAITYIKDVTFNTSLPDAEILKMTKPPFVMEKWIVGIAEDQTVECTVTYENDVRTPKLTKHVHSIQWDRTKPQDEVKAVQLAIQTLFSSSEGNPGSRSDSSADCQWLDTLRMRQIASHTSLQRSQSNPILGSPFFPYFANQDSYAAAVRRTQTPVKYQQITPSINPSRSSSPTQYGLSRNFSSLNLSSRDSGFSSLNDSSSERGRYSDRSRNKYYRGSVSLNSSPKGRYGGKSQHSTPSRERYSGKFYRLPQSALNTHQSPDFKRSPNDHDRRVPRTIPGMPLHPETASKAGEEESRVSEGGWTKVEYRKKTHRQLSAKTSKERTRGNYRGRRNF

Target ORF information:

RefSeq Version	XM_006499997.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 20 (Map3k20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499997.3

ORF Insert Sequence:

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 
GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 
GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 
TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 
CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 
GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 
GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 
AAGCTGACGG AGCAATCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCGTGGACT 
GAAGACGATG TGTATTTTTG GGTTCAGCAG CTCGTCAGAA AAGGCGAGTC TTCAGTAGAG 
ATGAGTGGAT ACGCAAGTTT GTTTAAGGAG AACAACATCA CAGGCAAGCG GTTGCTGCTT 
CTGGAAGAGG AAGATCTGAA AGACATGGGC ATCGTCTCCA AGGGACACAT CATTCACTTT 
AAGTCAGCTA TTGAGAAGCT AACCCACGAT TATCTGAACC TGTTTCACTT CCCACCACTG 
ATTAAGGATT CAGGAGGGGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA GCCAGGAACT GGCCCACAGG ATTGCAAATG GAAAATGTAT 
ATGGAGATGG ATGGGGACGA AGTTGCAATC ACGTACATAA AAGATGTGAC TTTCAACACG 
AGCCTCCCTG ATGCAGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATCGTGGGAA TAGCGGAGGA TCAGACTGTG GAGTGCACGG TCACCTATGA GAATGATGTC 
AGAACACCAA AACTCACTAA GCACGTGCAC TCCATACAGT GGGACAGAAC GAAGCCTCAG 
GATGAGGTGA AGGCGGTCCA GCTTGCCATT CAGACTCTGT TCTCCAGCTC AGAGGGCAAC 
CCTGGCAGCA GATCCGACTC CAGTGCGGAC TGCCAATGGT TAGACACTCT GCGCATGCGG 
CAGATTGCAT CCCACACTTC TCTACAGCGT TCCCAAAGCA ACCCTATCTT GGGGTCTCCT 
TTCTTCCCTT ACTTTGCCAA CCAGGATTCC TATGCTGCTG CGGTGAGGAG GACCCAGACT 
CCGGTGAAGT ACCAGCAGAT TACACCAAGC ATCAACCCTT CCCGGAGCTC CTCCCCCACG 
CAGTATGGGC TGTCCAGAAA CTTCTCCTCC CTGAACCTCA GCTCCAGGGA CAGCGGCTTC 
TCCAGCCTCA ATGACAGCTC CTCCGAGAGG GGCCGCTACT CAGACCGAAG CAGAAACAAG 
TACTACCGTG GTAGTGTGTC CCTCAATTCT TCCCCGAAAG GAAGATATGG TGGGAAAAGT 
CAGCATTCCA CACCTTCAAG AGAAAGATAT TCTGGAAAGT TCTACAGGCT GCCCCAGTCA 
GCGCTGAACA CTCATCAGTC CCCTGACTTC AAGAGGAGCC CAAATGACCA TGACCGCCGT 
GTGCCCAGGA CCATACCCGG GATGCCTCTG CACCCTGAGA CTGCCTCCAA AGCAGGGGAA 
GAGGAGAGCA GGGTGAGCGA AGGGGGCTGG ACTAAAGTGG AATATCGGAA GAAGACACAC 
AGGCAACTTT CGGCCAAGAC TAGCAAGGAG AGAACCCGTG GCAACTACCG TGGGCGGCGG 
AATTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Novel Zak Knockout Mouse with a Defective Ribotoxic Stress Response.
Toxins8(9)(2016 09)
Jandhyala DM,Wong J,Mantis NJ,Magun BE,Leong JM,Thorpe CM

Exome sequencing and CRISPR/Cas genome editing identify mutations of ZAK as a cause of limb defects in humans and mice.
Genome research26(2)183-91(2016 Feb)
Spielmann M,Kakar N,Tayebi N,Leettola C,N?rnberg G,Sowada N,Lupi??ez DG,Harabula I,Fl?ttmann R,Horn D,Chan WL,Wittler L,Yilmaz R,Altm?ller J,Thiele H,van Bokhoven H,Schwartz CE,N?rnberg P,Bowie JU,Ahmad J,Kubisch C,Mundlos S,Borck G

Searching for genomic region of high-fat diet-induced type 2 diabetes in mouse chromosome 2 by analysis of congenic strains.
PloS one9(5)e96271(2014)
Kobayashi M,Ohno T,Ihara K,Murai A,Kumazawa M,Hoshino H,Iwanaga K,Iwai H,Hamana Y,Ito M,Ohno K,Horio F

Identification of transcripts with enriched expression in the developing and adult pancreas.
Genome biology9(6)R99(2008)
Hoffman BG,Zavaglia B,Witzsche J,Ruiz de Algara T,Beach M,Hoodless PA,Jones SJ,Marra MA,Helgason CD

Complete inhibition of anisomycin and UV radiation but not cytokine induced JNK and p38 activation by an aryl-substituted dihydropyrrolopyrazole quinoline and mixed lineage kinase 7 small interfering RNA.
The Journal of biological chemistry280(19)19298-305(2005 May)
Wang X,Mader MM,Toth JE,Yu X,Jin N,Campbell RM,Smallwood JK,Christe ME,Chatterjee A,Goodson T Jr,Vlahos CJ,Matter WF,Bloem LJ

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Map3k20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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