Twsg1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Twsg1
Entrez Gene ID	65960
Full Name	twisted gastrulation BMP signaling modulator 1
Synonyms	1810013J15Rik,9030422N06Rik,AW552143,D17Ertd403e,Tsg,Twg
General protein information	Preferred Names twisted gastrulation BMP signaling modulator 1 Names twisted gastrulation protein homolog 1 twisted gastrulation homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 E1.1\|17 35.26 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_023053.3	NP_075540.1	twisted gastrulation protein homolog 1 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TWSG1	NP_989529.1
Xenopus tropicalis (tropical clawed frog)	twsg1	NP_001006748.1
Bos taurus (cattle)	TWSG1	NP_001069557.1
Homo sapiens (human)	TWSG1	NP_065699.1
Danio rerio (zebrafish)	twsg1a	NP_571872.1
Drosophila melanogaster (fruit fly)	tsg	NP_511135.1
Pan troglodytes (chimpanzee)	TWSG1	XP_001138675.1
Canis lupus familiaris (dog)	TWSG1	XP_547668.2
Mus musculus (house mouse)	Twsg1	NP_075540.1
	TWSG1	XP_002800917.1
Rattus norvegicus (Norway rat)	Twsg1	NP_001102281.1

Gene Ontology
Bibliography

Related articles in PubMed

Villification in the mouse: Bmp signals control intestinal villus patterning.
Walton KD, Whidden M, Kolterud ?, Shoffner SK, Czerwinski MJ, Kushwaha J, Parmar N, Chandhrasekhar D, Freddo AM, Schnell S, Gumucio DL
Development (Cambridge, England)143(3)427-36(2016 Feb)

The Function of Twisted Gastrulation in Regulating Osteoclast Differentiation is Dependent on BMP Binding.
Huntley R, Davydova J, Petryk A, Billington CJ Jr, Jensen ED, Mansky KC, Gopalakrishnan R
Journal of cellular biochemistry116(10)2239-46(2015 Oct)

Impact of retinoic acid exposure on midfacial shape variation and manifestation of holoprosencephaly in Twsg1 mutant mice.
Billington CJ Jr, Schmidt B, Marcucio RS, Hallgrimsson B, Gopalakrishnan R, Petryk A
Disease models & mechanisms8(2)139-46(2015 Feb)

Twisted gastrulation, a BMP antagonist, exacerbates podocyte injury.
Yamada S, Nakamura J, Asada M, Takase M, Matsusaka T, Iguchi T, Yamada R, Tanaka M, Higashi AY, Okuda T, Asada N, Fukatsu A, Kawachi H, Graf D, Muso E, Kita T, Kimura T, Pastan I, Economides AN, Yanagita M
PloS one9(2)e89135(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results show that BMP binding is required for TWSG1-mediated inhibition of osteoclast formation and function, and validate the critical functional regions within the TWSG1 protein for these interactions
Title: The Function of Twisted Gastrulation in Regulating Osteoclast Differentiation is Dependent on BMP Binding.

The goal of this study was to examine whether mice carrying a mutation in a gene encoding Twsg1, which is associated with a low penetrance of holoprosencephaly, are sensitized to retinoic acid teratogenesis.
Title: Impact of retinoic acid exposure on midfacial shape variation and manifestation of holoprosencephaly in Twsg1 mutant mice.

BMP-binding protein twisted gastrulation is required in mammary gland epithelium for normal ductal elongation and myoepithelial compartmentalization.
Title: BMP-binding protein twisted gastrulation is required in mammary gland epithelium for normal ductal elongation and myoepithelial compartmentalization.

Data show that Bmp4, Bmp7, Gremlin and Twisted gastrulation (Twsg1) are all expressed in the thymus and expression was clearly different for each gene investigated.
Title: Compartmentalization of bone morphogenetic proteins and their antagonists in lymphoid progenitors and supporting microenvironments and functional implications.

suggesting that Twsg1 expression in B cells serves to interpret BMP signals on a per-cell basis
Title: Involvement of twisted gastrulation in T cell-independent plasma cell production.

The following Twsg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Twsg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00102	NM_023053.3 Latest version!	Mus musculus twisted gastrulation BMP signaling modulator 1 (Twsg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Twsg1 ( NM_023053.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075540.1 Mus musculus twisted gastrulation BMP signaling modulator 1 (Twsg1), mRNA.

Price & Availability↑

CloneID	OMu00102
Clone ID Related Accession (Same CDS sequence)	NM_023053.3
Accession Version	NM_023053.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	twisted gastrulation protein homolog 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ165542.1, AK049646.1 and CF727906.1. On Dec 10, 2009 this sequence version replaced NM_023053.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK049646.1, AK033504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTCAC ACTATATTGT GCTAGCTCTA GCCTCCCTGA CGTTCCTGCT GTGTCTCCCC 
GTGTCCCAGA GCTGTAACAA AGCACTCTGT GCCAGCGATG TGAGCAAATG CCTCATTCAG 
GAGCTCTGCC AGTGCCGGCC TGGAGAAGGG AACTGCCCCT GCTGTAAGGA GTGCATGCTG 
TGCCTCGGGG CCCTGTGGGA CGAGTGCTGC GACTGTGTCG GTATGTGCAA CCCTCGGAAT 
TACAGCGACA CCCCGCCCAC ATCCAAGAGC ACCGTGGAGG AGCTGCACGA GCCCATTCCG 
TCCCTGTTCA GGGCGCTGAC GGAGGGCGAC ACCCAGCTGA ACTGGAACAT CGTCTCCTTC 
CCTGTGGCAG AGGAGCTGTC ACACCATGAA AACCTAGTCT CCTTCCTAGA AACTGTGAAC 
CAGCTGCACC ACCAAAACGT GTCTGTTCCC AGCAACAATG TCCACGCCCC CTTCCCCAGC 
GACAAAGAGC GCATGTGCAC AGTGGTTTAC TTTGATGACT GCATGTCCAT CCACCAGTGT 
AAGATATCCT GCGAATCCAT GGGTGCATCC AAGTATCGCT GGTTTCACAA CGCCTGCTGC 
GAGTGCATCG GTCCAGAGTG CATTGACTAT GGGAGTAAAA CTGTCAAGTG TATGAACTGC 
ATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075540.1
CDS	159..827
Translation	MKSHYIVLALASLTFLLCLPVSQSCNKALCASDVSKCLIQELCQCRPGEGNCPCCKECMLCLGALWDECCDCVGMCNPRNYSDTPPTSKSTVEELHEPIPSLFRALTEGDTQLNWNIVSFPVAEELSHHENLVSFLETVNQLHHQNVSVPSNNVHAPFPSDKERMCTVVYFDDCMSIHQCKISCESMGASKYRWFHNACCECIGPECIDYGSKTVKCMNCMF

Target ORF information:

RefSeq Version	NM_023053.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus twisted gastrulation BMP signaling modulator 1 (Twsg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023053.3

ORF Insert Sequence:

ATGAAGTCAC ACTATATTGT GCTAGCTCTA GCCTCCCTGA CGTTCCTGCT GTGTCTCCCC 
GTGTCCCAGA GCTGTAACAA AGCACTCTGT GCCAGCGATG TGAGCAAATG CCTCATTCAG 
GAGCTCTGCC AGTGCCGGCC TGGAGAAGGG AACTGCCCCT GCTGTAAGGA GTGCATGCTG 
TGCCTCGGGG CCCTGTGGGA CGAGTGCTGC GACTGTGTCG GTATGTGCAA CCCTCGGAAT 
TACAGCGACA CCCCGCCCAC ATCCAAGAGC ACCGTGGAGG AGCTGCACGA GCCCATTCCG 
TCCCTGTTCA GGGCGCTGAC GGAGGGCGAC ACCCAGCTGA ACTGGAACAT CGTCTCCTTC 
CCTGTGGCAG AGGAGCTGTC ACACCATGAA AACCTAGTCT CCTTCCTAGA AACTGTGAAC 
CAGCTGCACC ACCAAAACGT GTCTGTTCCC AGCAACAATG TCCACGCCCC CTTCCCCAGC 
GACAAAGAGC GCATGTGCAC AGTGGTTTAC TTTGATGACT GCATGTCCAT CCACCAGTGT 
AAGATATCCT GCGAATCCAT GGGTGCATCC AAGTATCGCT GGTTTCACAA CGCCTGCTGC 
GAGTGCATCG GTCCAGAGTG CATTGACTAT GGGAGTAAAA CTGTCAAGTG TATGAACTGC 
ATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Villification in the mouse: Bmp signals control intestinal villus patterning.
Development (Cambridge, England)143(3)427-36(2016 Feb)
Walton KD,Whidden M,Kolterud ?,Shoffner SK,Czerwinski MJ,Kushwaha J,Parmar N,Chandhrasekhar D,Freddo AM,Schnell S,Gumucio DL

The Function of Twisted Gastrulation in Regulating Osteoclast Differentiation is Dependent on BMP Binding.
Journal of cellular biochemistry116(10)2239-46(2015 Oct)
Huntley R,Davydova J,Petryk A,Billington CJ Jr,Jensen ED,Mansky KC,Gopalakrishnan R

Impact of retinoic acid exposure on midfacial shape variation and manifestation of holoprosencephaly in Twsg1 mutant mice.
Disease models & mechanisms8(2)139-46(2015 Feb)
Billington CJ Jr,Schmidt B,Marcucio RS,Hallgrimsson B,Gopalakrishnan R,Petryk A

Twisted gastrulation, a BMP antagonist, exacerbates podocyte injury.
PloS one9(2)e89135(2014)
Yamada S,Nakamura J,Asada M,Takase M,Matsusaka T,Iguchi T,Yamada R,Tanaka M,Higashi AY,Okuda T,Asada N,Fukatsu A,Kawachi H,Graf D,Muso E,Kita T,Kimura T,Pastan I,Economides AN,Yanagita M

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Twsg1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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