Ndfip1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndfip1
Entrez Gene ID	65113
Full Name	Nedd4 family interacting protein 1
Synonyms	0610010M22Rik,N4wbp5
General protein information	Preferred Names Nedd4 family interacting protein 1 Names NEDD4 family-interacting protein 1 NEDD4 WW domain-binding protein 5 Nedd4 WW binding protein 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526149.1	XP_006526212.1	NEDD4 family-interacting protein 1 isoform X1
NM_022996.1	NP_075372.1	NEDD4 family-interacting protein 1
NM_001355749.1	NP_001342678.1	NEDD4 family-interacting protein 1
NM_022996.2	NP_075372.1	NEDD4 family-interacting protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NDFIP1	XP_002689314.3
Drosophila melanogaster (fruit fly)	Ndfip	NP_730291.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005801	XP_001237719.2
Xenopus tropicalis (tropical clawed frog)	ndfip1	NP_001027505.1
Canis lupus familiaris (dog)	NDFIP1	XP_005617965.1
	NDFIP1	XP_001095360.1
Mus musculus (house mouse)	Ndfip1	NP_075372.1
Rattus norvegicus (Norway rat)	Ndfip1	NP_001013077.1
Danio rerio (zebrafish)	ndfip1	NP_955960.1
Homo sapiens (human)	NDFIP1	NP_085048.1
Pan troglodytes (chimpanzee)	NDFIP1	XP_001153016.2

Gene Ontology
Bibliography

Related articles in PubMed

Ndfip1 restricts mTORC1 signalling and glycolysis in regulatory T cells to prevent autoinflammatory disease.
Layman AAK, Deng G, O'Leary CE, Tadros S, Thomas RM, Dybas JM, Moser EK, Wells AD, Doliba NM, Oliver PM
Nature communications815677(2017 Jun)

NDFIP allows NEDD4/NEDD4L-induced AQP2 ubiquitination and degradation.
Trimpert C, Wesche D, de Groot T, Pimentel Rodriguez MM, Wong V, van den Berg DTM, Cheval L, Ariza CA, Doucet A, Stagljar I, Deen PMT
PloS one12(9)e0183774(2017)

Ndfip1 restricts Th17 cell potency by limiting lineage stability and proinflammatory cytokine production.
Layman AAK, Sprout SL, Phillips D, Oliver PM
Scientific reports739649(2017 01)

The Nedd4-2/Ndfip1 axis is a negative regulator of IgE-mediated mast cell activation.
Yip KH, Kolesnikoff N, Hauschild N, Biggs L, Lopez AF, Galli SJ, Kumar S, Grimbaldeston MA
Nature communications713198(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate thar Janus kinase 1 (Jak1) degradation is dependent on Nedd4 family interacting proteins Ndfip1/Ndfip2.
Title: Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells.

Silencing of Ndfip1 inhibited cytokine-induced apoptosis of pancreatic islets and promoted glucose-stimulated insulin secretion. These effects were associated with an increase in the cellular content of JunB, a potent inhibitor of ER stress and apoptosis.
Title: Nedd4 family interacting protein 1 (Ndfip1) promotes death of pancreatic beta cells.

transgene expression of Ndfip1 in the developing brain increased nuclear Pten and lengthened the cell cycle of neuronal progenitors, resulting in microencephaly.
Title: Ndfip1 represses cell proliferation by controlling Pten localization and signaling specificity.

Ndfip1 is required during stress for ubiquitinating and trafficking BRAT1 into the nucleus.
Title: Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.

Ndfip1 regulates itch ligase activity and airway inflammation via UbcH7.
Title: Ndfip1 regulates itch ligase activity and airway inflammation via UbcH7.

The following Ndfip1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndfip1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54726	NM_022996.2 Latest version!	Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu54726	NM_001355749.1 Latest version!	Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu54726	XM_006526149.1 Latest version!	Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22003	NM_022996.1 Latest version!	Mus musculus Nedd4 family interacting protein 1 (Ndfip1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndfip1 ( NM_001355749.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342678.1 Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu54726
Clone ID Related Accession (Same CDS sequence)	XM_006526149.1 , NM_001355749.1 , NM_022996.2
Accession Version	NM_001355749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	NEDD4 family-interacting protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC134576.3 and AK050560.1. On Sep 26, 2017 this sequence version replaced XM_006526149.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK050560.1, SRR7345562.414626.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTGG CGTTGGCGGC GCTGGCGGCC GTGGAGCCGG CCTGCGGCAG CGGGTACCAG 
CAGTTGCAGA ATGAAGAGGA GCCTGGGGAA CCTGAGCAGA CTGCAGGTGA TGCTCCTCCA 
CCATACAGCA GCATCACGGC AGAGAGTGCA GCATATTTTG ACTACAAAGA TGAATCTGGA 
TTTCCAAAGC CCCCATCGTA TAATGTGGCT ACAACACTGC CCAGTTATGA CGAGGCTGAG 
AGAACCAAGA CTGAAGCTAC GATCCCTTTG GTTCCTGGAA GAGATGAAGA TTTTGTGGGC 
CGGGATGATT TTGATGATAC TGACCAGCTG AGGATAGGAA ACGATGGGAT TTTTATGTTA 
ACTTTTTTCA TGGCATTCCT CTTCAACTGG ATTGGGTTTT TCTTGTCTTT TTGCCTGACC 
ACCTCAGCTG CGGGAAGGTA TGGGGCCATC TCAGGATTTG GTCTTTCTCT AATTAAGTGG 
ATCCTTATTG TCAGGTTTTC CACCTATTTC CCTGGATACT TTGATGGCCA GTACTGGCTC 
TGGTGGGTGT TCTTGGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTACGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342678.1
CDS	208..873
Translation	MALALAALAAVEPACGSGYQQLQNEEEPGEPEQTAGDAPPPYSSITAESAAYFDYKDESGFPKPPSYNVATTLPSYDEAERTKTEATIPLVPGRDEDFVGRDDFDDTDQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLTTSAAGRYGAISGFGLSLIKWILIVRFSTYFPGYFDGQYWLWWVFLVLGFLLFLRGFINYAKVRKMPETFSNLPRTRVLFIY

Target ORF information:

RefSeq Version	NM_001355749.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355749.1

ORF Insert Sequence:

ATGGCCTTGG CGTTGGCGGC GCTGGCGGCC GTGGAGCCGG CCTGCGGCAG CGGGTACCAG 
CAGTTGCAGA ATGAAGAGGA GCCTGGGGAA CCTGAGCAGA CTGCAGGTGA TGCTCCTCCA 
CCATACAGCA GCATCACGGC AGAGAGTGCA GCATATTTTG ACTACAAAGA TGAATCTGGA 
TTTCCAAAGC CCCCATCGTA TAATGTGGCT ACAACACTGC CCAGTTATGA CGAGGCTGAG 
AGAACCAAGA CTGAAGCTAC GATCCCTTTG GTTCCTGGAA GAGATGAAGA TTTTGTGGGC 
CGGGATGATT TTGATGATAC TGACCAGCTG AGGATAGGAA ACGATGGGAT TTTTATGTTA 
ACTTTTTTCA TGGCATTCCT CTTCAACTGG ATTGGGTTTT TCTTGTCTTT TTGCCTGACC 
ACCTCAGCTG CGGGAAGGTA TGGGGCCATC TCAGGATTTG GTCTTTCTCT AATTAAGTGG 
ATCCTTATTG TCAGGTTTTC CACCTATTTC CCTGGATACT TTGATGGCCA GTACTGGCTC 
TGGTGGGTGT TCTTGGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTACGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndfip1 ( NM_022996.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075372.1 Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu54726
Clone ID Related Accession (Same CDS sequence)	XM_006526149.1 , NM_001355749.1 , NM_022996.2
Accession Version	NM_022996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	NEDD4 family-interacting protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC134576.3. On Sep 26, 2017 this sequence version replaced NM_022996.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC020359.1, AF220209.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTGG CGTTGGCGGC GCTGGCGGCC GTGGAGCCGG CCTGCGGCAG CGGGTACCAG 
CAGTTGCAGA ATGAAGAGGA GCCTGGGGAA CCTGAGCAGA CTGCAGGTGA TGCTCCTCCA 
CCATACAGCA GCATCACGGC AGAGAGTGCA GCATATTTTG ACTACAAAGA TGAATCTGGA 
TTTCCAAAGC CCCCATCGTA TAATGTGGCT ACAACACTGC CCAGTTATGA CGAGGCTGAG 
AGAACCAAGA CTGAAGCTAC GATCCCTTTG GTTCCTGGAA GAGATGAAGA TTTTGTGGGC 
CGGGATGATT TTGATGATAC TGACCAGCTG AGGATAGGAA ACGATGGGAT TTTTATGTTA 
ACTTTTTTCA TGGCATTCCT CTTCAACTGG ATTGGGTTTT TCTTGTCTTT TTGCCTGACC 
ACCTCAGCTG CGGGAAGGTA TGGGGCCATC TCAGGATTTG GTCTTTCTCT AATTAAGTGG 
ATCCTTATTG TCAGGTTTTC CACCTATTTC CCTGGATACT TTGATGGCCA GTACTGGCTC 
TGGTGGGTGT TCTTGGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTACGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075372.1
CDS	208..873
Translation	MALALAALAAVEPACGSGYQQLQNEEEPGEPEQTAGDAPPPYSSITAESAAYFDYKDESGFPKPPSYNVATTLPSYDEAERTKTEATIPLVPGRDEDFVGRDDFDDTDQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLTTSAAGRYGAISGFGLSLIKWILIVRFSTYFPGYFDGQYWLWWVFLVLGFLLFLRGFINYAKVRKMPETFSNLPRTRVLFIY

Target ORF information:

RefSeq Version	NM_022996.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022996.2

ORF Insert Sequence:

ATGGCCTTGG CGTTGGCGGC GCTGGCGGCC GTGGAGCCGG CCTGCGGCAG CGGGTACCAG 
CAGTTGCAGA ATGAAGAGGA GCCTGGGGAA CCTGAGCAGA CTGCAGGTGA TGCTCCTCCA 
CCATACAGCA GCATCACGGC AGAGAGTGCA GCATATTTTG ACTACAAAGA TGAATCTGGA 
TTTCCAAAGC CCCCATCGTA TAATGTGGCT ACAACACTGC CCAGTTATGA CGAGGCTGAG 
AGAACCAAGA CTGAAGCTAC GATCCCTTTG GTTCCTGGAA GAGATGAAGA TTTTGTGGGC 
CGGGATGATT TTGATGATAC TGACCAGCTG AGGATAGGAA ACGATGGGAT TTTTATGTTA 
ACTTTTTTCA TGGCATTCCT CTTCAACTGG ATTGGGTTTT TCTTGTCTTT TTGCCTGACC 
ACCTCAGCTG CGGGAAGGTA TGGGGCCATC TCAGGATTTG GTCTTTCTCT AATTAAGTGG 
ATCCTTATTG TCAGGTTTTC CACCTATTTC CCTGGATACT TTGATGGCCA GTACTGGCTC 
TGGTGGGTGT TCTTGGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTACGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndfip1 ( XM_006526149.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526212.1 Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54726
Clone ID Related Accession (Same CDS sequence)	XM_006526149.1 , NM_001355749.1 , NM_022996.2
Accession Version	XM_006526149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	NEDD4 family-interacting protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGG CGTTGGCGGC GCTGGCGGCC GTGGAGCCGG CCTGCGGCAG CGGGTACCAG 
CAGTTGCAGA ATGAAGAGGA GCCTGGGGAA CCTGAGCAGA CTGCAGGTGA TGCTCCTCCA 
CCATACAGCA GCATCACGGC AGAGAGTGCA GCATATTTTG ACTACAAAGA TGAATCTGGA 
TTTCCAAAGC CCCCATCGTA TAATGTGGCT ACAACACTGC CCAGTTATGA CGAGGCTGAG 
AGAACCAAGA CTGAAGCTAC GATCCCTTTG GTTCCTGGAA GAGATGAAGA TTTTGTGGGC 
CGGGATGATT TTGATGATAC TGACCAGCTG AGGATAGGAA ACGATGGGAT TTTTATGTTA 
ACTTTTTTCA TGGCATTCCT CTTCAACTGG ATTGGGTTTT TCTTGTCTTT TTGCCTGACC 
ACCTCAGCTG CGGGAAGGTA TGGGGCCATC TCAGGATTTG GTCTTTCTCT AATTAAGTGG 
ATCCTTATTG TCAGGTTTTC CACCTATTTC CCTGGATACT TTGATGGCCA GTACTGGCTC 
TGGTGGGTGT TCTTGGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTACGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526212.1
CDS	203..868
Translation	MALALAALAAVEPACGSGYQQLQNEEEPGEPEQTAGDAPPPYSSITAESAAYFDYKDESGFPKPPSYNVATTLPSYDEAERTKTEATIPLVPGRDEDFVGRDDFDDTDQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLTTSAAGRYGAISGFGLSLIKWILIVRFSTYFPGYFDGQYWLWWVFLVLGFLLFLRGFINYAKVRKMPETFSNLPRTRVLFIY

Target ORF information:

RefSeq Version	XM_006526149.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus Nedd4 family interacting protein 1 (Ndfip1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526149.1

ORF Insert Sequence:

ATGGCCTTGG CGTTGGCGGC GCTGGCGGCC GTGGAGCCGG CCTGCGGCAG CGGGTACCAG 
CAGTTGCAGA ATGAAGAGGA GCCTGGGGAA CCTGAGCAGA CTGCAGGTGA TGCTCCTCCA 
CCATACAGCA GCATCACGGC AGAGAGTGCA GCATATTTTG ACTACAAAGA TGAATCTGGA 
TTTCCAAAGC CCCCATCGTA TAATGTGGCT ACAACACTGC CCAGTTATGA CGAGGCTGAG 
AGAACCAAGA CTGAAGCTAC GATCCCTTTG GTTCCTGGAA GAGATGAAGA TTTTGTGGGC 
CGGGATGATT TTGATGATAC TGACCAGCTG AGGATAGGAA ACGATGGGAT TTTTATGTTA 
ACTTTTTTCA TGGCATTCCT CTTCAACTGG ATTGGGTTTT TCTTGTCTTT TTGCCTGACC 
ACCTCAGCTG CGGGAAGGTA TGGGGCCATC TCAGGATTTG GTCTTTCTCT AATTAAGTGG 
ATCCTTATTG TCAGGTTTTC CACCTATTTC CCTGGATACT TTGATGGCCA GTACTGGCTC 
TGGTGGGTGT TCTTGGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTACGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndfip1 ( NM_022996.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075372.1 Mus musculus Nedd4 family interacting protein 1 (Ndfip1), mRNA.

Price & Availability↑

CloneID	OMu22003
Clone ID Related Accession (Same CDS sequence)	NM_022996.1
Accession Version	NM_022996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Mus musculus(House mouse)
Product	NEDD4 family-interacting protein 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC026372.1. On or before May 4, 2006 this sequence version replaced XM_985952.1, XM_001052153.1, XM_988154.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026372.1, AF220209.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTGG CGTTGGCGGC GCTGGCGGCC GTGGAGCCGG CCTGCGGCAG CGGGTACCAG 
CAGTTGCAGA ATGAAGAGGA GCCTGGGGAA CCTGAGCAGA CTGCAGGTGA TGCTCCTCCA 
CCATACAGCA GCATCACTGC AGAGAGTGCA GCATATTTTG ACTACAAAGA TGAATCTGGA 
TTTCCAAAGC CCCCATCGTA TAATGTGGCT ACAACACTGC CCAGTTATGA CGAGGCTGAG 
AGAACCAAGA CTGAAGCTAC GATCCCTTTG GTTCCTGGAA GAGATGAAGA TTTTGTGGGC 
CGGGATGATT TTGATGATAC TGACCAGCTG AGGATAGGAA ACGATGGGAT TTTTATGTTA 
ACTTTTTTCA TGGCATTCCT CTTCAACTGG ATTGGGTTTT TCTTGTCTTT TTGCCTGACC 
ACCTCAGCTG CGGGAAGGTA TGGGGCCATC TCAGGATTTG GTCTTTCTCT AATTAAGTGG 
ATCCTTATTG TCAGGTTTTC CACCTATTTC CCTGGATACT TTGATGGCCA GTACTGGCTC 
TGGTGGGTGT TCTTGGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTACGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075372.1
CDS	140..805
Translation	MALALAALAAVEPACGSGYQQLQNEEEPGEPEQTAGDAPPPYSSITAESAAYFDYKDESGFPKPPSYNVATTLPSYDEAERTKTEATIPLVPGRDEDFVGRDDFDDTDQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLTTSAAGRYGAISGFGLSLIKWILIVRFSTYFPGYFDGQYWLWWVFLVLGFLLFLRGFINYAKVRKMPETFSNLPRTRVLFIY

Target ORF information:

RefSeq Version	NM_022996.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus Nedd4 family interacting protein 1 (Ndfip1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022996.1

ORF Insert Sequence:

ATGGCCTTGG CGTTGGCGGC GCTGGCGGCC GTGGAGCCGG CCTGCGGCAG CGGGTACCAG 
CAGTTGCAGA ATGAAGAGGA GCCTGGGGAA CCTGAGCAGA CTGCAGGTGA TGCTCCTCCA 
CCATACAGCA GCATCACTGC AGAGAGTGCA GCATATTTTG ACTACAAAGA TGAATCTGGA 
TTTCCAAAGC CCCCATCGTA TAATGTGGCT ACAACACTGC CCAGTTATGA CGAGGCTGAG 
AGAACCAAGA CTGAAGCTAC GATCCCTTTG GTTCCTGGAA GAGATGAAGA TTTTGTGGGC 
CGGGATGATT TTGATGATAC TGACCAGCTG AGGATAGGAA ACGATGGGAT TTTTATGTTA 
ACTTTTTTCA TGGCATTCCT CTTCAACTGG ATTGGGTTTT TCTTGTCTTT TTGCCTGACC 
ACCTCAGCTG CGGGAAGGTA TGGGGCCATC TCAGGATTTG GTCTTTCTCT AATTAAGTGG 
ATCCTTATTG TCAGGTTTTC CACCTATTTC CCTGGATACT TTGATGGCCA GTACTGGCTC 
TGGTGGGTGT TCTTGGTTTT AGGCTTTCTC CTGTTTCTCA GAGGATTTAT CAATTACGCA 
AAAGTTCGGA AGATGCCAGA AACTTTCTCA AATCTCCCCA GGACCAGAGT TCTCTTTATT 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ndfip1 restricts mTORC1 signalling and glycolysis in regulatory T cells to prevent autoinflammatory disease.
Nature communications815677(2017 Jun)
Layman AAK,Deng G,O'Leary CE,Tadros S,Thomas RM,Dybas JM,Moser EK,Wells AD,Doliba NM,Oliver PM

NDFIP allows NEDD4/NEDD4L-induced AQP2 ubiquitination and degradation.
PloS one12(9)e0183774(2017)
Trimpert C,Wesche D,de Groot T,Pimentel Rodriguez MM,Wong V,van den Berg DTM,Cheval L,Ariza CA,Doucet A,Stagljar I,Deen PMT

Ndfip1 restricts Th17 cell potency by limiting lineage stability and proinflammatory cytokine production.
Scientific reports739649(2017 01)
Layman AAK,Sprout SL,Phillips D,Oliver PM

The Nedd4-2/Ndfip1 axis is a negative regulator of IgE-mediated mast cell activation.
Nature communications713198(2016 Oct)
Yip KH,Kolesnikoff N,Hauschild N,Biggs L,Lopez AF,Galli SJ,Kumar S,Grimbaldeston MA

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Ndfip1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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