Nisch cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nisch
Entrez Gene ID	64652
Full Name	nischarin
Synonyms	1200007D05Rik,3202002H23Rik,AW494485,I-1,edsn
General protein information	Preferred Names nischarin Names nischarin I1R IR1 imidazoline receptor 1 imidazoline receptor I-1-like protein imidazoline-1 receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316115.1	XP_017171604.1	nischarin isoform X1
NM_001347583.1	NP_001334512.1	nischarin isoform 2
NM_022656.2	NP_073147.2	nischarin isoform 1
XM_017316115.2	XP_017171604.1	nischarin isoform X1
XM_006519353.2	XP_006519416.1	nischarin isoform X1
XM_017316116.1	XP_017171605.1	nischarin isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nisch	NP_073147.2
Danio rerio (zebrafish)	nisch	XP_002666276.2
Xenopus tropicalis (tropical clawed frog)	nisch	NP_001123817.1
Caenorhabditis elegans (roundworm)	CELE_F13E9.1	NP_502016.2
Gallus gallus (chicken)	NISCH	XP_004944436.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.
Gstrein T, Edwards A, P?istoupilov? A, Leca I, Breuss M, Pilat-Carotta S, Hansen AH, Tripathy R, Traunbauer AK, Hochstoeger T, Rosoklija G, Repic M, Landler L, Str?neck? V, D?rnberger G, Keane TM, Zuber J, Adams DJ, Flint J, Honzik T, Gut M, Beltran S, Mechtler K, Sherr E, Kmoch S, Gut I, Keays DA
Nature neuroscience(2018 Jan)

Nischarin inhibition alters energy metabolism by activating AMP-activated protein kinase.
Dong S, Baranwal S, Garcia A, Serrano-Gomez SJ, Eastlack S, Iwakuma T, Mercante D, Mauvais-Jarvis F, Alahari SK
The Journal of biological chemistry292(41)16833-16846(2017 10)

A mutation in Nischarin causes otitis media via LIMK1 and NF-?B pathways.
Crompton M, Purnell T, Tyrer HE, Parker A, Ball G, Hardisty-Hughes RE, Gale R, Williams D, Dean CH, Simon MM, Mallon AM, Wells S, Bhutta MF, Burton MJ, Tateossian H, Brown SDM
PLoS genetics13(8)e1006969(2017 Aug)

Inhibition of Nischarin Expression Promotes Neurite Outgrowth through Regulation of PAK Activity.
Ding Y, Li Y, Lu L, Zhang R, Zeng L, Wang L, Zhang X
PloS one10(12)e0144948(2015)

Improvement of obesity by activation of I1-imidazoline receptors in high fat diet-fed mice.
Chung HH, Cheng J
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(8)581-5(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate that Nisch is an important AMPK inhibitor and a critical regulator of energy homeostasis, including lipid and glucose metabolism.
Title: Nischarin inhibition alters energy metabolism by activating AMP-activated protein kinase.

We identified a significant genetic interaction between Nisch and Itga5; mice heterozygous for Itga5-null and homozygous for edison mutations display a significantly increased penetrance and severity of chronic otitis media.
Title: A mutation in Nischarin causes otitis media via LIMK1 and NF-?B pathways.

Nischarin inhibits neurite outgrowth by blocking PAK1 activation in neurons.
Title: Inhibition of Nischarin Expression Promotes Neurite Outgrowth through Regulation of PAK Activity.

Activation of I1-imidazoline receptor ameliorated energy intake and epididymal white adipose tissue accumulation.
Title: Improvement of obesity by activation of I1-imidazoline receptors in high fat diet-fed mice.

Data suggest that both IR1 and IR2 (imidazoline receptor 2) play roles in acquisition of behavioral responses in alcoholism; activation of IR1 and IR2 may serve as molecular targets in prevention (and possibly treatment) of alcoholism.
Title: Agmatine attenuates acquisition but not the expression of ethanol conditioned place preference in mice: a role for imidazoline receptors.

The following Nisch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nisch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69869	NM_001347583.1 Latest version!	Mus musculus nischarin (Nisch), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu50222	XM_017316115.2 Latest version!	Mus musculus nischarin (Nisch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu14197	NM_022656.2 Latest version!	Mus musculus nischarin (Nisch), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu50222	XM_006519353.2 Latest version!	Mus musculus nischarin (Nisch), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu50222	XM_017316116.1 Latest version!	Mus musculus nischarin (Nisch), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OMu50222	XM_017316115.1	Mus musculus nischarin (Nisch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nisch ( NM_001347583.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334512.1 Mus musculus nischarin (Nisch), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69869
Clone ID Related Accession (Same CDS sequence)	NM_001347583.1
Accession Version	NM_001347583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	nischarin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ106892.1, AK160990.1, AK004628.1, AC154446.2 and BY602009.1. On Dec 1, 2016 this sequence version replaced XM_006519354.2. Transcript Variant: This variant (2) lacks multiple exons and includes an alternate 3' terminal exon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003270.1, BC066838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGGGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTCGTGG GTTCTGAGCT CGTGGACACG TACACGGTTT ATGTCATCCA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGT TACAGTGATT TTCATGACCT ACATGAAAAG 
CTTGTGGCAG AGAGAAAAAT TGACAAATCT CTACTTCCAC CCAAAAAGAT AATTGGGAAG 
AACTCGAGAA GCTTGGTAGA GAAGAGGGAG AGAGACCTGG AGGTGTACCT CCAGACGCTC 
CTCACAACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAGTAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAGCAA 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAAC AGGGAAAGCC TACTTGTGCC AGTGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAT CTTAAGGTTT CAGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTAAGGA GCAGCTCTTG CCCTTTGATC TTTCAATATT CAAGTCTCTT 
CACCAGGTGG AGATAAGTCA TTGTGATGCC AAGCATATCC GAGGGCTGGT CACCTCCAAG 
CCCACTTTAG CCACAATGAG TGTTAGATTC TCAGCAACCT CAATGAAGGA AGTGCTTGCT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CTGCTACCCT CGGAGGCCCT 
GTGACTGCTA TCATCCCAAC ATGGCAAGCA CTGACTACTC TAGACCTGAG CCACAACAGC 
ATCTGTGAGA TCGACGAATC TGTGAAACTG ATCCCAAAGA TAGAGTACCT GGACCTGAGT 
CACAATGGAC TGCGAGTTGT GGATAACCTG CAGCACCTAT ACAACCTCGT GCACCTTGAC 
CTGTCCTACA ACAAGCTCTC CTCCCTGGAA GGCGTGCACA CTAAACTGGG CAATGTCAAA 
ACCCTAAACC TGGCAGGCAA CTTCTTAGAG AGTCTGAGCG GTCTGCACAA ACTCTACTCC 
TTGGTTAATG TGGACCTACG AGACAACCGG ATTGAGCAGT TGGATGAGGT CAAGAGCATT 
GGCAGCCTGC CATGTCTGGA GCGTTTGACC TTGCTGAACA ACCCTTTGAG CATCATCCCT 
GACTACCGGA CCAAGGTGCT TTCCCAGTTT GGAGAACGAG CCTCTGAGAT TTGTCTAGAT 
GATGTCGCAA CCACAGAGAA AGAGCTGGAC ACTGTGGAAG TGCTGAAGGC AATTCAGAAA 
GCCAAAGATG TCAAGTCCAA ACTGAGCAAC ACAGAAAAGA AGGCTGGTGA GGACTTCCGG 
CTCCCGCCTG CACCCTGCAT CAGACCCGGC GGCTCCCCTC CTGCAGCTCC CGCCTCAGCC 
TCCCTGCCTC AGCCGATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334512.1
CDS	165..1715
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKSLLPPKKIIGKNSRSLVEKRERDLEVYLQTLLTTFPDVAPRVLAHFLHFHLYEVNGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIKEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVTSKPTLATMSVRFSATSMKEVLAPEASEFDEWEPEGTATLGGPVTAIIPTWQALTTLDLSHNSICEIDESVKLIPKIEYLDLSHNGLRVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLESLSGLHKLYSLVNVDLRDNRIEQLDEVKSIGSLPCLERLTLLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSNTEKKAGEDFRLPPAPCIRPGGSPPAAPASASLPQPILSNQGILGDE

Target ORF information:

RefSeq Version	NM_001347583.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nischarin (Nisch), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347583.1

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGGGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTCGTGG GTTCTGAGCT CGTGGACACG TACACGGTTT ATGTCATCCA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGT TACAGTGATT TTCATGACCT ACATGAAAAG 
CTTGTGGCAG AGAGAAAAAT TGACAAATCT CTACTTCCAC CCAAAAAGAT AATTGGGAAG 
AACTCGAGAA GCTTGGTAGA GAAGAGGGAG AGAGACCTGG AGGTGTACCT CCAGACGCTC 
CTCACAACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAGTAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAGCAA 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAAC AGGGAAAGCC TACTTGTGCC AGTGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAT CTTAAGGTTT CAGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTAAGGA GCAGCTCTTG CCCTTTGATC TTTCAATATT CAAGTCTCTT 
CACCAGGTGG AGATAAGTCA TTGTGATGCC AAGCATATCC GAGGGCTGGT CACCTCCAAG 
CCCACTTTAG CCACAATGAG TGTTAGATTC TCAGCAACCT CAATGAAGGA AGTGCTTGCT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CTGCTACCCT CGGAGGCCCT 
GTGACTGCTA TCATCCCAAC ATGGCAAGCA CTGACTACTC TAGACCTGAG CCACAACAGC 
ATCTGTGAGA TCGACGAATC TGTGAAACTG ATCCCAAAGA TAGAGTACCT GGACCTGAGT 
CACAATGGAC TGCGAGTTGT GGATAACCTG CAGCACCTAT ACAACCTCGT GCACCTTGAC 
CTGTCCTACA ACAAGCTCTC CTCCCTGGAA GGCGTGCACA CTAAACTGGG CAATGTCAAA 
ACCCTAAACC TGGCAGGCAA CTTCTTAGAG AGTCTGAGCG GTCTGCACAA ACTCTACTCC 
TTGGTTAATG TGGACCTACG AGACAACCGG ATTGAGCAGT TGGATGAGGT CAAGAGCATT 
GGCAGCCTGC CATGTCTGGA GCGTTTGACC TTGCTGAACA ACCCTTTGAG CATCATCCCT 
GACTACCGGA CCAAGGTGCT TTCCCAGTTT GGAGAACGAG CCTCTGAGAT TTGTCTAGAT 
GATGTCGCAA CCACAGAGAA AGAGCTGGAC ACTGTGGAAG TGCTGAAGGC AATTCAGAAA 
GCCAAAGATG TCAAGTCCAA ACTGAGCAAC ACAGAAAAGA AGGCTGGTGA GGACTTCCGG 
CTCCCGCCTG CACCCTGCAT CAGACCCGGC GGCTCCCCTC CTGCAGCTCC CGCCTCAGCC 
TCCCTGCCTC AGCCGATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_017316115.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171604.1 Mus musculus nischarin (Nisch), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50222
Clone ID Related Accession (Same CDS sequence)	XM_017316115.1 , XM_017316115.2 , XM_006519353.2 , XM_017316116.1
Accession Version	XM_017316115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTAC AGGAAGAGGC CCTGGCCAGC AGCCTCTCAT CCACGGATAG TCTGCCACCC 
GAGGACCACC GGCCCATTGC CCGAGCCTGC TCTGACTCCT TGGAATCTAT CCCTGCAGGA 
CAGGTGGCCT CTGATGATCT GAGGGATGTG CCAGGAGCTG TTGGCGGCGT GAGCCCAGAT 
CATGCAGAGC CAGAGGTTCA GGTGGTGCCT GGGTCCGGCC AGATCATCTT CCTGCCCTTC 
ACTTGCATTG GCTACACAGC CACCAACCAG GACTTTATCC AGCGCCTCAG CACACTGATC 
CGCCAGGCCA TTGAACGGCA ACTCCCTGCC TGGATTGAGG CTGCCAACCA GCGCGAGGAA 
GCCCATGGTG AGCAGGGCGA GGAGGAAGAA GAGGAAGAGG AAGAGGAGGA TGTTGCTGAG 
AACCGCTACT TTGAAATGGG ACCCCCAGAC GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA 
GAAGAGGATG AGGAAGACGA GGACGAGGAG GCGGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCCCTGGGCG CCGATGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA AGGCAGCATC CGCCAGTTCG CTGCCTGCCT TGTGCTCACT 
GACTTCGGCA TCGCAGTCTT TGAGATCCCA CACCAAGAGT CAAGAGGCAG CAGCCAGCAC 
ATCCTCTCAT CCCTGCGTTT TGTCTTCTGC TTCCCACATG GCGACCTCAC GGAGTTTGGC 
TTCCTCATGC CCGAGCTGTG TCTGGTGCTC AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCGG ATGCTGCCAA CTTACACGAG TTCCATGCTG ATCTACGCTC CTGCTTTGCA 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC ATCCTCTACG GCAGCCACAC CACCCTGCAG 
GAGTTCTTGC GCCAGCTGCT CACCTTCTAC AAGGTGGCCG GGGGCTCTCA GGAGCGCAGC 
CAGGGCTGCT TCCCTGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTCCA GACCCCTGCC 
GGGGACTATT CAGGCAATAT CGAATGGGCC AGCTGCACGC TGTGCTCGGC GGTGCGGCGC 
TCCTGCTGCG CGCCCTCGGA GGCCGTCAAG TCCGCTGCCA TCCCCTACTG GCTGCTGCTC 
ACATCCCAGC ATCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA TCGAGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTTAGCCGCG TCCCGCTCTC TACCGTGCTG 
CTAGACCCCA CTCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCTGATGG CCATGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTTACC GCCATCTTTG TGCTGCCCCA CGAGAAATTC 
CACTTCCTGC GAGTCTACAA TCAGCTACGC GCCTCACTGC AGGACCTGAA GACTGTGGTC 
ATCTCCAAGA ATCCTTCAGC TAAGCCAAGA AATCAGCCTG CCAAGAGCAG GGCCAGTGCT 
GAGCAGCGGC TACAGGAGAC CCCAGCAGAC GCTCCGGCTC CAGCTGCAGT CCCACCAACA 
GCTTCAGCTC CAGCCCCCGC AGAGGCCTTG GCTCCAGATC TGGCTCCTGT ACAGGCCCCA 
GGAGAGGACC GAGGTCTAAC TTCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCGGCCCCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCGCAGCAG AGGCTCCAGC TGCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCTTCA 
GCAGAGGCTC CAGCTCCAAA CCAGGCTCCT GCTCCAGCAA GGGGTCCCGC TCCAGCAAGG 
GGTCCCGCTC CAGCAGGGGG TCCCGCTCCA GCAGAGGCCC TGGCACAAGC AGAAGTCCCT 
GCCCAGTACC CAAGTGAGCG CCTAATCCAG TCCACATCTG AAGAGAATCA GATCCCTTCT 
CACTTGCCAG TATGCCCATC ACTCCAGCAC ATTGCCCGTC TTCGGGGGAG AGCCATCATT 
GACCTCTTCC ACAACAGCAT TGCTGAGGTT GAAAATGAGG AGCTGAGGCA CCTCCTGTGG 
TCATCAGTGG TGTTCTACCA GACCCCGGGG CTAGAAGTGA CCGCCTGTGT GCTGCTCTCT 
AGCAAGGCTG TGTACTTCAT ACTGCATGAT GGTCTCCGCC GCTACTTCTC TGAACCACTG 
CAGGATTTCT GGCACCAGAA AAACACCGAC TATAACAACA GTCCTTTCCA CGTCTCTCAG 
TGCTTTGTGT TGAAACTCAG TGACCTGCAG TCAGTCAACG TCGGCCTTTT CGACCAGTAC 
TTCCGGCTGA CGGGCTCCTC CCCGACGCAG GTGGTCACGT GCTTGACTCG CGACAGCTAC 
CTGACGCACT GCTTCCTCCA GCATCTGATG CTTGTGCTGT CCTCCCTGGA GCGCACACCC 
TCGCCTGAGC CTGTTGACAA GGACTTCTAC TCAGAATTTG GGGACAAGAA TACAGGGAAA 
ATGGAGAACT ATGAGCTGAT CCATTCCAGC CGCGTCAAGT TCACCTACCC CAGTGAGGAA 
GAGGTTGGGG ACCTGACCTA TATTGTCGCA CAGAAGATGG CTGATCCTGC AAAGAATCCA 
GCCCTCAGCA TCTTACTGTA CATCCAGGCC TTCCAGGTGG TCACACCACA CCTTGGGCGG 
GGCAGGGGCC CACTGCGCCC TAAGACGCTG CTCCTGACCA GCGCCGAGAT CTTCCTCCTG 
GATGAGGACT ACATCCACTA TCCATTGCCT GAATTTGCCA AAGAGCCACC GCAGAGGGAC 
AGATACCGGC TAGACGATGG CCGCCGGGTC CGGGATTTGG ACCGGGTGCT CATGGGCTAC 
TATCCCTACC CACAGGCCCT CACTCTTGTT TTTGATGACA CACAGGGTCA CGACCTCATG 
GGGAGTGTCA CCCTGGACCA CTTTGGGGAG ATGCCAGGTG GTCCTGGCAG GGTTGGCCAG 
GGCCGAGAGG TACAGTGGCA GGTGTTTGTC CCCAGTGCCG AGAGCCGAGA AAAGCTCATC 
TCACTGCTCG CACGACAGTG GGAAGCTCTT TGTGGCAGGG AGCTGCCTGT GGAGCTCACT 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171604.1
CDS	5006..8251
Translation	MFVQEEALASSLSSTDSLPPEDHRPIARACSDSLESIPAGQVASDDLRDVPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEEDVAENRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTTLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTPAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVISKNPSAKPRNQPAKSRASAEQRLQETPADAPAPAAVPPTASAPAPAEALAPDLAPVQAPGEDRGLTSAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAPAEAPAAAEAPAAAEAPAAAEAPAAAEAPASAEAPAPNQAPAPARGPAPARGPAPAGGPAPAEALAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHNSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSILLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRVGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_017316115.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nischarin (Nisch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316115.2

ORF Insert Sequence:

ATGTTTGTAC AGGAAGAGGC CCTGGCCAGC AGCCTCTCAT CCACGGATAG TCTGCCACCC 
GAGGACCACC GGCCCATTGC CCGAGCCTGC TCTGACTCCT TGGAATCTAT CCCTGCAGGA 
CAGGTGGCCT CTGATGATCT GAGGGATGTG CCAGGAGCTG TTGGCGGCGT GAGCCCAGAT 
CATGCAGAGC CAGAGGTTCA GGTGGTGCCT GGGTCCGGCC AGATCATCTT CCTGCCCTTC 
ACTTGCATTG GCTACACAGC CACCAACCAG GACTTTATCC AGCGCCTCAG CACACTGATC 
CGCCAGGCCA TTGAACGGCA ACTCCCTGCC TGGATTGAGG CTGCCAACCA GCGCGAGGAA 
GCCCATGGTG AGCAGGGCGA GGAGGAAGAA GAGGAAGAGG AAGAGGAGGA TGTTGCTGAG 
AACCGCTACT TTGAAATGGG ACCCCCAGAC GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA 
GAAGAGGATG AGGAAGACGA GGACGAGGAG GCGGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCCCTGGGCG CCGATGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA AGGCAGCATC CGCCAGTTCG CTGCCTGCCT TGTGCTCACT 
GACTTCGGCA TCGCAGTCTT TGAGATCCCA CACCAAGAGT CAAGAGGCAG CAGCCAGCAC 
ATCCTCTCAT CCCTGCGTTT TGTCTTCTGC TTCCCACATG GCGACCTCAC GGAGTTTGGC 
TTCCTCATGC CCGAGCTGTG TCTGGTGCTC AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCGG ATGCTGCCAA CTTACACGAG TTCCATGCTG ATCTACGCTC CTGCTTTGCA 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC ATCCTCTACG GCAGCCACAC CACCCTGCAG 
GAGTTCTTGC GCCAGCTGCT CACCTTCTAC AAGGTGGCCG GGGGCTCTCA GGAGCGCAGC 
CAGGGCTGCT TCCCTGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTCCA GACCCCTGCC 
GGGGACTATT CAGGCAATAT CGAATGGGCC AGCTGCACGC TGTGCTCGGC GGTGCGGCGC 
TCCTGCTGCG CGCCCTCGGA GGCCGTCAAG TCCGCTGCCA TCCCCTACTG GCTGCTGCTC 
ACATCCCAGC ATCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA TCGAGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTTAGCCGCG TCCCGCTCTC TACCGTGCTG 
CTAGACCCCA CTCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCTGATGG CCATGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTTACC GCCATCTTTG TGCTGCCCCA CGAGAAATTC 
CACTTCCTGC GAGTCTACAA TCAGCTACGC GCCTCACTGC AGGACCTGAA GACTGTGGTC 
ATCTCCAAGA ATCCTTCAGC TAAGCCAAGA AATCAGCCTG CCAAGAGCAG GGCCAGTGCT 
GAGCAGCGGC TACAGGAGAC CCCAGCAGAC GCTCCGGCTC CAGCTGCAGT CCCACCAACA 
GCTTCAGCTC CAGCCCCCGC AGAGGCCTTG GCTCCAGATC TGGCTCCTGT ACAGGCCCCA 
GGAGAGGACC GAGGTCTAAC TTCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCGGCCCCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCGCAGCAG AGGCTCCAGC TGCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCTTCA 
GCAGAGGCTC CAGCTCCAAA CCAGGCTCCT GCTCCAGCAA GGGGTCCCGC TCCAGCAAGG 
GGTCCCGCTC CAGCAGGGGG TCCCGCTCCA GCAGAGGCCC TGGCACAAGC AGAAGTCCCT 
GCCCAGTACC CAAGTGAGCG CCTAATCCAG TCCACATCTG AAGAGAATCA GATCCCTTCT 
CACTTGCCAG TATGCCCATC ACTCCAGCAC ATTGCCCGTC TTCGGGGGAG AGCCATCATT 
GACCTCTTCC ACAACAGCAT TGCTGAGGTT GAAAATGAGG AGCTGAGGCA CCTCCTGTGG 
TCATCAGTGG TGTTCTACCA GACCCCGGGG CTAGAAGTGA CCGCCTGTGT GCTGCTCTCT 
AGCAAGGCTG TGTACTTCAT ACTGCATGAT GGTCTCCGCC GCTACTTCTC TGAACCACTG 
CAGGATTTCT GGCACCAGAA AAACACCGAC TATAACAACA GTCCTTTCCA CGTCTCTCAG 
TGCTTTGTGT TGAAACTCAG TGACCTGCAG TCAGTCAACG TCGGCCTTTT CGACCAGTAC 
TTCCGGCTGA CGGGCTCCTC CCCGACGCAG GTGGTCACGT GCTTGACTCG CGACAGCTAC 
CTGACGCACT GCTTCCTCCA GCATCTGATG CTTGTGCTGT CCTCCCTGGA GCGCACACCC 
TCGCCTGAGC CTGTTGACAA GGACTTCTAC TCAGAATTTG GGGACAAGAA TACAGGGAAA 
ATGGAGAACT ATGAGCTGAT CCATTCCAGC CGCGTCAAGT TCACCTACCC CAGTGAGGAA 
GAGGTTGGGG ACCTGACCTA TATTGTCGCA CAGAAGATGG CTGATCCTGC AAAGAATCCA 
GCCCTCAGCA TCTTACTGTA CATCCAGGCC TTCCAGGTGG TCACACCACA CCTTGGGCGG 
GGCAGGGGCC CACTGCGCCC TAAGACGCTG CTCCTGACCA GCGCCGAGAT CTTCCTCCTG 
GATGAGGACT ACATCCACTA TCCATTGCCT GAATTTGCCA AAGAGCCACC GCAGAGGGAC 
AGATACCGGC TAGACGATGG CCGCCGGGTC CGGGATTTGG ACCGGGTGCT CATGGGCTAC 
TATCCCTACC CACAGGCCCT CACTCTTGTT TTTGATGACA CACAGGGTCA CGACCTCATG 
GGGAGTGTCA CCCTGGACCA CTTTGGGGAG ATGCCAGGTG GTCCTGGCAG GGTTGGCCAG 
GGCCGAGAGG TACAGTGGCA GGTGTTTGTC CCCAGTGCCG AGAGCCGAGA AAAGCTCATC 
TCACTGCTCG CACGACAGTG GGAAGCTCTT TGTGGCAGGG AGCTGCCTGT GGAGCTCACT 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( NM_022656.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_073147.2 Mus musculus nischarin (Nisch), transcript variant 1, mRNA.

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CloneID	OMu14197
Clone ID Related Accession (Same CDS sequence)	NM_022656.2
Accession Version	NM_022656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	nischarin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ106892.1, AK122412.1, BC089329.1, AC154446.2 and BP761152.1. On Sep 8, 2006 this sequence version replaced NM_022656.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGGGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTCGTGG GTTCTGAGCT CGTGGACACG TACACGGTTT ATGTCATCCA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGT TACAGTGATT TTCATGACCT ACATGAAAAG 
CTTGTGGCAG AGAGAAAAAT TGACAAATCT CTACTTCCAC CCAAAAAGAT AATTGGGAAG 
AACTCGAGAA GCTTGGTAGA GAAGAGGGAG AGAGACCTGG AGGTGTACCT CCAGACGCTC 
CTCACAACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAGTAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAGCAA 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAAC AGGGAAAGCC TACTTGTGCC AGTGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAT CTTAAGGTTT CAGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTAAGGA GCAGCTCTTG CCCTTTGATC TTTCAATATT CAAGTCTCTT 
CACCAGGTGG AGATAAGTCA TTGTGATGCC AAGCATATCC GAGGGCTGGT CACCTCCAAG 
CCCACTTTAG CCACAATGAG TGTTAGATTC TCAGCAACCT CAATGAAGGA AGTGCTTGCT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CTGCTACCCT CGGAGGCCCT 
GTGACTGCTA TCATCCCAAC ATGGCAAGCA CTGACTACTC TAGACCTGAG CCACAACAGC 
ATCTGTGAGA TCGACGAATC TGTGAAACTG ATCCCAAAGA TAGAGTACCT GGACCTGAGT 
CACAATGGAC TGCGAGTTGT GGATAACCTG CAGCACCTAT ACAACCTCGT GCACCTTGAC 
CTGTCCTACA ACAAGCTCTC CTCCCTGGAA GGCGTGCACA CTAAACTGGG CAATGTCAAA 
ACCCTAAACC TGGCAGGCAA CTTCTTAGAG AGTCTGAGCG GTCTGCACAA ACTCTACTCC 
TTGGTTAATG TGGACCTACG AGACAACCGG ATTGAGCAGT TGGATGAGGT CAAGAGCATT 
GGCAGCCTGC CATGTCTGGA GCGTTTGACC TTGCTGAACA ACCCTTTGAG CATCATCCCT 
GACTACCGGA CCAAGGTGCT TTCCCAGTTT GGAGAACGAG CCTCTGAGAT TTGTCTAGAT 
GATGTCGCAA CCACAGAGAA AGAGCTGGAC ACTGTGGAAG TGCTGAAGGC AATTCAGAAA 
GCCAAAGATG TCAAGTCCAA ACTGAGCAAC ACAGAAAAGA AGGCTGGTGA GGACTTCCGG 
CTCCCGCCTG CACCCTGCAT CAGACCCGGC GGCTCCCCTC CTGCAGCTCC CGCCTCAGCC 
TCCCTGCCTC AGCCGATCCT CTCCAACCAA GGTATCATGT TTGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGTCTG CCACCCGAGG ACCACCGGCC CATTGCCCGA 
GCCTGCTCTG ACTCCTTGGA ATCTATCCCT GCAGGACAGG TGGCCTCTGA TGATCTGAGG 
GATGTGCCAG GAGCTGTTGG CGGCGTGAGC CCAGATCATG CAGAGCCAGA GGTTCAGGTG 
GTGCCTGGGT CCGGCCAGAT CATCTTCCTG CCCTTCACTT GCATTGGCTA CACAGCCACC 
AACCAGGACT TTATCCAGCG CCTCAGCACA CTGATCCGCC AGGCCATTGA ACGGCAACTC 
CCTGCCTGGA TTGAGGCTGC CAACCAGCGC GAGGAAGCCC ATGGTGAGCA GGGCGAGGAG 
GAAGAAGAGG AAGAGGAAGA GGAGGATGTT GCTGAGAACC GCTACTTTGA AATGGGACCC 
CCAGACGCAG AGGAAGAGGA GGGGAGTGGC CAGGGAGAAG AGGATGAGGA AGACGAGGAC 
GAGGAGGCGG AGGAGGAGCG CCTGGCTCTG GAGTGGGCCC TGGGCGCCGA TGAGGACTTC 
CTGCTGGAGC ACATCCGCAT CCTCAAGGTG CTCTGGTGCT TCCTGATCCA CGTGCAAGGC 
AGCATCCGCC AGTTCGCTGC CTGCCTTGTG CTCACTGACT TCGGCATCGC AGTCTTTGAG 
ATCCCACACC AAGAGTCAAG AGGCAGCAGC CAGCACATCC TCTCATCCCT GCGTTTTGTC 
TTCTGCTTCC CACATGGCGA CCTCACGGAG TTTGGCTTCC TCATGCCCGA GCTGTGTCTG 
GTGCTCAAGG TGCGGCACAG TGAGAACACG CTCTTCATCA TCTCGGATGC TGCCAACTTA 
CACGAGTTCC ATGCTGATCT ACGCTCCTGC TTTGCACCGC AGCACATGGC CATGCTGTGC 
AGCCCCATCC TCTACGGCAG CCACACCACC CTGCAGGAGT TCTTGCGCCA GCTGCTCACC 
TTCTACAAGG TGGCCGGGGG CTCTCAGGAG CGCAGCCAGG GCTGCTTCCC TGTCTACCTG 
GTCTACAGTG ACAAGCGCAT GGTCCAGACC CCTGCCGGGG ACTATTCAGG CAATATCGAA 
TGGGCCAGCT GCACGCTGTG CTCGGCGGTG CGGCGCTCCT GCTGCGCGCC CTCGGAGGCC 
GTCAAGTCCG CTGCCATCCC CTACTGGCTG CTGCTCACAT CCCAGCATCT CAACGTCATC 
AAGGCCGACT TCAACCCCAT GCCCAATCGA GGCACCCACA ACTGCCGCAA CCGCAACAGC 
TTCAAGCTTA GCCGCGTCCC GCTCTCTACC GTGCTGCTAG ACCCCACTCG CAGCTGCACC 
CAGCCACGGG GTGCCTTCGC TGATGGCCAT GTGCTCGAGC TGCTCGTGGG CTACCGCTTC 
GTTACCGCCA TCTTTGTGCT GCCCCACGAG AAATTCCACT TCCTGCGAGT CTACAATCAG 
CTACGCGCCT CACTGCAGGA CCTGAAGACT GTGGTCATCT CCAAGAATCC TTCAGCTAAG 
CCAAGAAATC AGCCTGCCAA GAGCAGGGCC AGTGCTGAGC AGCGGCTACA GGAGACCCCA 
GCAGACGCTC CGGCTCCAGC TGCAGTCCCA CCAACAGCTT CAGCTCCAGC CCCCGCAGAG 
GCCTTGGCTC CAGATCTGGC TCCTGTACAG GCCCCAGGAG AGGACCGAGG TCTAACTTCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCCCAGCAG AGGCTCCAGC CGCAGCAGAG GCTCCGGCCG CAGCAGAGGC TCCAGCTGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCTTCAGCAG AGGCTCCAGC TCCAAACCAG 
GCTCCTGCTC CAGCAAGGGG TCCCGCTCCA GCAAGGGGTC CCGCTCCAGC AGGGGGTCCC 
GCTCCAGCAG AGGCCCTGGC ACAAGCAGAA GTCCCTGCCC AGTACCCAAG TGAGCGCCTA 
ATCCAGTCCA CATCTGAAGA GAATCAGATC CCTTCTCACT TGCCAGTATG CCCATCACTC 
CAGCACATTG CCCGTCTTCG GGGGAGAGCC ATCATTGACC TCTTCCACAA CAGCATTGCT 
GAGGTTGAAA ATGAGGAGCT GAGGCACCTC CTGTGGTCAT CAGTGGTGTT CTACCAGACC 
CCGGGGCTAG AAGTGACCGC CTGTGTGCTG CTCTCTAGCA AGGCTGTGTA CTTCATACTG 
CATGATGGTC TCCGCCGCTA CTTCTCTGAA CCACTGCAGG ATTTCTGGCA CCAGAAAAAC 
ACCGACTATA ACAACAGTCC TTTCCACGTC TCTCAGTGCT TTGTGTTGAA ACTCAGTGAC 
CTGCAGTCAG TCAACGTCGG CCTTTTCGAC CAGTACTTCC GGCTGACGGG CTCCTCCCCG 
ACGCAGGTGG TCACGTGCTT GACTCGCGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAT 
CTGATGCTTG TGCTGTCCTC CCTGGAGCGC ACACCCTCGC CTGAGCCTGT TGACAAGGAC 
TTCTACTCAG AATTTGGGGA CAAGAATACA GGGAAAATGG AGAACTATGA GCTGATCCAT 
TCCAGCCGCG TCAAGTTCAC CTACCCCAGT GAGGAAGAGG TTGGGGACCT GACCTATATT 
GTCGCACAGA AGATGGCTGA TCCTGCAAAG AATCCAGCCC TCAGCATCTT ACTGTACATC 
CAGGCCTTCC AGGTGGTCAC ACCACACCTT GGGCGGGGCA GGGGCCCACT GCGCCCTAAG 
ACGCTGCTCC TGACCAGCGC CGAGATCTTC CTCCTGGATG AGGACTACAT CCACTATCCA 
TTGCCTGAAT TTGCCAAAGA GCCACCGCAG AGGGACAGAT ACCGGCTAGA CGATGGCCGC 
CGGGTCCGGG ATTTGGACCG GGTGCTCATG GGCTACTATC CCTACCCACA GGCCCTCACT 
CTTGTTTTTG ATGACACACA GGGTCACGAC CTCATGGGGA GTGTCACCCT GGACCACTTT 
GGGGAGATGC CAGGTGGTCC TGGCAGGGTT GGCCAGGGCC GAGAGGTACA GTGGCAGGTG 
TTTGTCCCCA GTGCCGAGAG CCGAGAAAAG CTCATCTCAC TGCTCGCACG ACAGTGGGAA 
GCTCTTTGTG GCAGGGAGCT GCCTGTGGAG CTCACTGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073147.2
CDS	165..4946
Translation	MAAATLSFGPEREAEPAKEARVVGSELVDTYTVYVIQVTDGNHEWTIKHRYSDFHDLHEKLVAERKIDKSLLPPKKIIGKNSRSLVEKRERDLEVYLQTLLTTFPDVAPRVLAHFLHFHLYEVNGVTAALAEELFEKGEQLLGAGEVFAIRPLQLYAITEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIKEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVTSKPTLATMSVRFSATSMKEVLAPEASEFDEWEPEGTATLGGPVTAIIPTWQALTTLDLSHNSICEIDESVKLIPKIEYLDLSHNGLRVVDNLQHLYNLVHLDLSYNKLSSLEGVHTKLGNVKTLNLAGNFLESLSGLHKLYSLVNVDLRDNRIEQLDEVKSIGSLPCLERLTLLNNPLSIIPDYRTKVLSQFGERASEICLDDVATTEKELDTVEVLKAIQKAKDVKSKLSNTEKKAGEDFRLPPAPCIRPGGSPPAAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLPPEDHRPIARACSDSLESIPAGQVASDDLRDVPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEEDVAENRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTTLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTPAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVISKNPSAKPRNQPAKSRASAEQRLQETPADAPAPAAVPPTASAPAPAEALAPDLAPVQAPGEDRGLTSAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAPAEAPAAAEAPAAAEAPAAAEAPAAAEAPASAEAPAPNQAPAPARGPAPARGPAPAGGPAPAEALAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHNSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSILLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRVGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	NM_022656.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nischarin (Nisch), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022656.2

ORF Insert Sequence:

ATGGCGGCTG CGACACTCAG CTTCGGCCCT GAGCGGGAGG CCGAGCCTGC CAAGGAGGCG 
CGCGTCGTGG GTTCTGAGCT CGTGGACACG TACACGGTTT ATGTCATCCA GGTTACCGAT 
GGCAACCATG AGTGGACGAT CAAACACCGT TACAGTGATT TTCATGACCT ACATGAAAAG 
CTTGTGGCAG AGAGAAAAAT TGACAAATCT CTACTTCCAC CCAAAAAGAT AATTGGGAAG 
AACTCGAGAA GCTTGGTAGA GAAGAGGGAG AGAGACCTGG AGGTGTACCT CCAGACGCTC 
CTCACAACCT TCCCTGATGT GGCTCCCAGA GTTCTGGCCC ACTTCCTGCA TTTTCACCTC 
TATGAAGTAA ATGGTGTCAC TGCAGCACTG GCTGAAGAAC TCTTTGAAAA AGGAGAGCAA 
CTTCTAGGAG CCGGTGAGGT CTTTGCCATC AGGCCCTTGC AGCTCTATGC TATCACAGAA 
CAGCTGCAAC AGGGAAAGCC TACTTGTGCC AGTGGTGATG CCAAGACTGA CCTTGGACAC 
ATCCTAGACT TCACCTGTCG CCTTAAGTAT CTTAAGGTTT CAGGCACAGA AGGACCTTTT 
GGGACCAGCA ATATTAAGGA GCAGCTCTTG CCCTTTGATC TTTCAATATT CAAGTCTCTT 
CACCAGGTGG AGATAAGTCA TTGTGATGCC AAGCATATCC GAGGGCTGGT CACCTCCAAG 
CCCACTTTAG CCACAATGAG TGTTAGATTC TCAGCAACCT CAATGAAGGA AGTGCTTGCT 
CCTGAAGCCT CAGAATTTGA TGAGTGGGAG CCTGAAGGCA CTGCTACCCT CGGAGGCCCT 
GTGACTGCTA TCATCCCAAC ATGGCAAGCA CTGACTACTC TAGACCTGAG CCACAACAGC 
ATCTGTGAGA TCGACGAATC TGTGAAACTG ATCCCAAAGA TAGAGTACCT GGACCTGAGT 
CACAATGGAC TGCGAGTTGT GGATAACCTG CAGCACCTAT ACAACCTCGT GCACCTTGAC 
CTGTCCTACA ACAAGCTCTC CTCCCTGGAA GGCGTGCACA CTAAACTGGG CAATGTCAAA 
ACCCTAAACC TGGCAGGCAA CTTCTTAGAG AGTCTGAGCG GTCTGCACAA ACTCTACTCC 
TTGGTTAATG TGGACCTACG AGACAACCGG ATTGAGCAGT TGGATGAGGT CAAGAGCATT 
GGCAGCCTGC CATGTCTGGA GCGTTTGACC TTGCTGAACA ACCCTTTGAG CATCATCCCT 
GACTACCGGA CCAAGGTGCT TTCCCAGTTT GGAGAACGAG CCTCTGAGAT TTGTCTAGAT 
GATGTCGCAA CCACAGAGAA AGAGCTGGAC ACTGTGGAAG TGCTGAAGGC AATTCAGAAA 
GCCAAAGATG TCAAGTCCAA ACTGAGCAAC ACAGAAAAGA AGGCTGGTGA GGACTTCCGG 
CTCCCGCCTG CACCCTGCAT CAGACCCGGC GGCTCCCCTC CTGCAGCTCC CGCCTCAGCC 
TCCCTGCCTC AGCCGATCCT CTCCAACCAA GGTATCATGT TTGTACAGGA AGAGGCCCTG 
GCCAGCAGCC TCTCATCCAC GGATAGTCTG CCACCCGAGG ACCACCGGCC CATTGCCCGA 
GCCTGCTCTG ACTCCTTGGA ATCTATCCCT GCAGGACAGG TGGCCTCTGA TGATCTGAGG 
GATGTGCCAG GAGCTGTTGG CGGCGTGAGC CCAGATCATG CAGAGCCAGA GGTTCAGGTG 
GTGCCTGGGT CCGGCCAGAT CATCTTCCTG CCCTTCACTT GCATTGGCTA CACAGCCACC 
AACCAGGACT TTATCCAGCG CCTCAGCACA CTGATCCGCC AGGCCATTGA ACGGCAACTC 
CCTGCCTGGA TTGAGGCTGC CAACCAGCGC GAGGAAGCCC ATGGTGAGCA GGGCGAGGAG 
GAAGAAGAGG AAGAGGAAGA GGAGGATGTT GCTGAGAACC GCTACTTTGA AATGGGACCC 
CCAGACGCAG AGGAAGAGGA GGGGAGTGGC CAGGGAGAAG AGGATGAGGA AGACGAGGAC 
GAGGAGGCGG AGGAGGAGCG CCTGGCTCTG GAGTGGGCCC TGGGCGCCGA TGAGGACTTC 
CTGCTGGAGC ACATCCGCAT CCTCAAGGTG CTCTGGTGCT TCCTGATCCA CGTGCAAGGC 
AGCATCCGCC AGTTCGCTGC CTGCCTTGTG CTCACTGACT TCGGCATCGC AGTCTTTGAG 
ATCCCACACC AAGAGTCAAG AGGCAGCAGC CAGCACATCC TCTCATCCCT GCGTTTTGTC 
TTCTGCTTCC CACATGGCGA CCTCACGGAG TTTGGCTTCC TCATGCCCGA GCTGTGTCTG 
GTGCTCAAGG TGCGGCACAG TGAGAACACG CTCTTCATCA TCTCGGATGC TGCCAACTTA 
CACGAGTTCC ATGCTGATCT ACGCTCCTGC TTTGCACCGC AGCACATGGC CATGCTGTGC 
AGCCCCATCC TCTACGGCAG CCACACCACC CTGCAGGAGT TCTTGCGCCA GCTGCTCACC 
TTCTACAAGG TGGCCGGGGG CTCTCAGGAG CGCAGCCAGG GCTGCTTCCC TGTCTACCTG 
GTCTACAGTG ACAAGCGCAT GGTCCAGACC CCTGCCGGGG ACTATTCAGG CAATATCGAA 
TGGGCCAGCT GCACGCTGTG CTCGGCGGTG CGGCGCTCCT GCTGCGCGCC CTCGGAGGCC 
GTCAAGTCCG CTGCCATCCC CTACTGGCTG CTGCTCACAT CCCAGCATCT CAACGTCATC 
AAGGCCGACT TCAACCCCAT GCCCAATCGA GGCACCCACA ACTGCCGCAA CCGCAACAGC 
TTCAAGCTTA GCCGCGTCCC GCTCTCTACC GTGCTGCTAG ACCCCACTCG CAGCTGCACC 
CAGCCACGGG GTGCCTTCGC TGATGGCCAT GTGCTCGAGC TGCTCGTGGG CTACCGCTTC 
GTTACCGCCA TCTTTGTGCT GCCCCACGAG AAATTCCACT TCCTGCGAGT CTACAATCAG 
CTACGCGCCT CACTGCAGGA CCTGAAGACT GTGGTCATCT CCAAGAATCC TTCAGCTAAG 
CCAAGAAATC AGCCTGCCAA GAGCAGGGCC AGTGCTGAGC AGCGGCTACA GGAGACCCCA 
GCAGACGCTC CGGCTCCAGC TGCAGTCCCA CCAACAGCTT CAGCTCCAGC CCCCGCAGAG 
GCCTTGGCTC CAGATCTGGC TCCTGTACAG GCCCCAGGAG AGGACCGAGG TCTAACTTCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCCCAGCAG AGGCTCCAGC CGCAGCAGAG GCTCCGGCCG CAGCAGAGGC TCCAGCTGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCTTCAGCAG AGGCTCCAGC TCCAAACCAG 
GCTCCTGCTC CAGCAAGGGG TCCCGCTCCA GCAAGGGGTC CCGCTCCAGC AGGGGGTCCC 
GCTCCAGCAG AGGCCCTGGC ACAAGCAGAA GTCCCTGCCC AGTACCCAAG TGAGCGCCTA 
ATCCAGTCCA CATCTGAAGA GAATCAGATC CCTTCTCACT TGCCAGTATG CCCATCACTC 
CAGCACATTG CCCGTCTTCG GGGGAGAGCC ATCATTGACC TCTTCCACAA CAGCATTGCT 
GAGGTTGAAA ATGAGGAGCT GAGGCACCTC CTGTGGTCAT CAGTGGTGTT CTACCAGACC 
CCGGGGCTAG AAGTGACCGC CTGTGTGCTG CTCTCTAGCA AGGCTGTGTA CTTCATACTG 
CATGATGGTC TCCGCCGCTA CTTCTCTGAA CCACTGCAGG ATTTCTGGCA CCAGAAAAAC 
ACCGACTATA ACAACAGTCC TTTCCACGTC TCTCAGTGCT TTGTGTTGAA ACTCAGTGAC 
CTGCAGTCAG TCAACGTCGG CCTTTTCGAC CAGTACTTCC GGCTGACGGG CTCCTCCCCG 
ACGCAGGTGG TCACGTGCTT GACTCGCGAC AGCTACCTGA CGCACTGCTT CCTCCAGCAT 
CTGATGCTTG TGCTGTCCTC CCTGGAGCGC ACACCCTCGC CTGAGCCTGT TGACAAGGAC 
TTCTACTCAG AATTTGGGGA CAAGAATACA GGGAAAATGG AGAACTATGA GCTGATCCAT 
TCCAGCCGCG TCAAGTTCAC CTACCCCAGT GAGGAAGAGG TTGGGGACCT GACCTATATT 
GTCGCACAGA AGATGGCTGA TCCTGCAAAG AATCCAGCCC TCAGCATCTT ACTGTACATC 
CAGGCCTTCC AGGTGGTCAC ACCACACCTT GGGCGGGGCA GGGGCCCACT GCGCCCTAAG 
ACGCTGCTCC TGACCAGCGC CGAGATCTTC CTCCTGGATG AGGACTACAT CCACTATCCA 
TTGCCTGAAT TTGCCAAAGA GCCACCGCAG AGGGACAGAT ACCGGCTAGA CGATGGCCGC 
CGGGTCCGGG ATTTGGACCG GGTGCTCATG GGCTACTATC CCTACCCACA GGCCCTCACT 
CTTGTTTTTG ATGACACACA GGGTCACGAC CTCATGGGGA GTGTCACCCT GGACCACTTT 
GGGGAGATGC CAGGTGGTCC TGGCAGGGTT GGCCAGGGCC GAGAGGTACA GTGGCAGGTG 
TTTGTCCCCA GTGCCGAGAG CCGAGAAAAG CTCATCTCAC TGCTCGCACG ACAGTGGGAA 
GCTCTTTGTG GCAGGGAGCT GCCTGTGGAG CTCACTGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_017316115.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171604.1 Mus musculus nischarin (Nisch), transcript variant X1, mRNA.

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CloneID	OMu50222
Clone ID Related Accession (Same CDS sequence)	XM_017316115.1 , XM_017316115.2 , XM_006519353.2 , XM_017316116.1
Accession Version	XM_017316115.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTAC AGGAAGAGGC CCTGGCCAGC AGCCTCTCAT CCACGGATAG TCTGCCACCC 
GAGGACCACC GGCCCATTGC CCGAGCCTGC TCTGACTCCT TGGAATCTAT CCCTGCAGGA 
CAGGTGGCCT CTGATGATCT GAGGGATGTG CCAGGAGCTG TTGGCGGCGT GAGCCCAGAT 
CATGCAGAGC CAGAGGTTCA GGTGGTGCCT GGGTCCGGCC AGATCATCTT CCTGCCCTTC 
ACTTGCATTG GCTACACAGC CACCAACCAG GACTTTATCC AGCGCCTCAG CACACTGATC 
CGCCAGGCCA TTGAACGGCA ACTCCCTGCC TGGATTGAGG CTGCCAACCA GCGCGAGGAA 
GCCCATGGTG AGCAGGGCGA GGAGGAAGAA GAGGAAGAGG AAGAGGAGGA TGTTGCTGAG 
AACCGCTACT TTGAAATGGG ACCCCCAGAC GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA 
GAAGAGGATG AGGAAGACGA GGACGAGGAG GCGGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCCCTGGGCG CCGATGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA AGGCAGCATC CGCCAGTTCG CTGCCTGCCT TGTGCTCACT 
GACTTCGGCA TCGCAGTCTT TGAGATCCCA CACCAAGAGT CAAGAGGCAG CAGCCAGCAC 
ATCCTCTCAT CCCTGCGTTT TGTCTTCTGC TTCCCACATG GCGACCTCAC GGAGTTTGGC 
TTCCTCATGC CCGAGCTGTG TCTGGTGCTC AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCGG ATGCTGCCAA CTTACACGAG TTCCATGCTG ATCTACGCTC CTGCTTTGCA 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC ATCCTCTACG GCAGCCACAC CACCCTGCAG 
GAGTTCTTGC GCCAGCTGCT CACCTTCTAC AAGGTGGCCG GGGGCTCTCA GGAGCGCAGC 
CAGGGCTGCT TCCCTGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTCCA GACCCCTGCC 
GGGGACTATT CAGGCAATAT CGAATGGGCC AGCTGCACGC TGTGCTCGGC GGTGCGGCGC 
TCCTGCTGCG CGCCCTCGGA GGCCGTCAAG TCCGCTGCCA TCCCCTACTG GCTGCTGCTC 
ACATCCCAGC ATCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA TCGAGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTTAGCCGCG TCCCGCTCTC TACCGTGCTG 
CTAGACCCCA CTCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCTGATGG CCATGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTTACC GCCATCTTTG TGCTGCCCCA CGAGAAATTC 
CACTTCCTGC GAGTCTACAA TCAGCTACGC GCCTCACTGC AGGACCTGAA GACTGTGGTC 
ATCTCCAAGA ATCCTTCAGC TAAGCCAAGA AATCAGCCTG CCAAGAGCAG GGCCAGTGCT 
GAGCAGCGGC TACAGGAGAC CCCAGCAGAC GCTCCGGCTC CAGCTGCAGT CCCACCAACA 
GCTTCAGCTC CAGCCCCCGC AGAGGCCTTG GCTCCAGATC TGGCTCCTGT ACAGGCCCCA 
GGAGAGGACC GAGGTCTAAC TTCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCGGCCCCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCGCAGCAG AGGCTCCAGC TGCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCTTCA 
GCAGAGGCTC CAGCTCCAAA CCAGGCTCCT GCTCCAGCAA GGGGTCCCGC TCCAGCAAGG 
GGTCCCGCTC CAGCAGGGGG TCCCGCTCCA GCAGAGGCCC TGGCACAAGC AGAAGTCCCT 
GCCCAGTACC CAAGTGAGCG CCTAATCCAG TCCACATCTG AAGAGAATCA GATCCCTTCT 
CACTTGCCAG TATGCCCATC ACTCCAGCAC ATTGCCCGTC TTCGGGGGAG AGCCATCATT 
GACCTCTTCC ACAACAGCAT TGCTGAGGTT GAAAATGAGG AGCTGAGGCA CCTCCTGTGG 
TCATCAGTGG TGTTCTACCA GACCCCGGGG CTAGAAGTGA CCGCCTGTGT GCTGCTCTCT 
AGCAAGGCTG TGTACTTCAT ACTGCATGAT GGTCTCCGCC GCTACTTCTC TGAACCACTG 
CAGGATTTCT GGCACCAGAA AAACACCGAC TATAACAACA GTCCTTTCCA CGTCTCTCAG 
TGCTTTGTGT TGAAACTCAG TGACCTGCAG TCAGTCAACG TCGGCCTTTT CGACCAGTAC 
TTCCGGCTGA CGGGCTCCTC CCCGACGCAG GTGGTCACGT GCTTGACTCG CGACAGCTAC 
CTGACGCACT GCTTCCTCCA GCATCTGATG CTTGTGCTGT CCTCCCTGGA GCGCACACCC 
TCGCCTGAGC CTGTTGACAA GGACTTCTAC TCAGAATTTG GGGACAAGAA TACAGGGAAA 
ATGGAGAACT ATGAGCTGAT CCATTCCAGC CGCGTCAAGT TCACCTACCC CAGTGAGGAA 
GAGGTTGGGG ACCTGACCTA TATTGTCGCA CAGAAGATGG CTGATCCTGC AAAGAATCCA 
GCCCTCAGCA TCTTACTGTA CATCCAGGCC TTCCAGGTGG TCACACCACA CCTTGGGCGG 
GGCAGGGGCC CACTGCGCCC TAAGACGCTG CTCCTGACCA GCGCCGAGAT CTTCCTCCTG 
GATGAGGACT ACATCCACTA TCCATTGCCT GAATTTGCCA AAGAGCCACC GCAGAGGGAC 
AGATACCGGC TAGACGATGG CCGCCGGGTC CGGGATTTGG ACCGGGTGCT CATGGGCTAC 
TATCCCTACC CACAGGCCCT CACTCTTGTT TTTGATGACA CACAGGGTCA CGACCTCATG 
GGGAGTGTCA CCCTGGACCA CTTTGGGGAG ATGCCAGGTG GTCCTGGCAG GGTTGGCCAG 
GGCCGAGAGG TACAGTGGCA GGTGTTTGTC CCCAGTGCCG AGAGCCGAGA AAAGCTCATC 
TCACTGCTCG CACGACAGTG GGAAGCTCTT TGTGGCAGGG AGCTGCCTGT GGAGCTCACT 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171604.1
CDS	5004..8249
Translation	MFVQEEALASSLSSTDSLPPEDHRPIARACSDSLESIPAGQVASDDLRDVPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEEDVAENRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTTLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTPAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVISKNPSAKPRNQPAKSRASAEQRLQETPADAPAPAAVPPTASAPAPAEALAPDLAPVQAPGEDRGLTSAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAPAEAPAAAEAPAAAEAPAAAEAPAAAEAPASAEAPAPNQAPAPARGPAPARGPAPAGGPAPAEALAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHNSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSILLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRVGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_017316115.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nischarin (Nisch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316115.1

ORF Insert Sequence:

ATGTTTGTAC AGGAAGAGGC CCTGGCCAGC AGCCTCTCAT CCACGGATAG TCTGCCACCC 
GAGGACCACC GGCCCATTGC CCGAGCCTGC TCTGACTCCT TGGAATCTAT CCCTGCAGGA 
CAGGTGGCCT CTGATGATCT GAGGGATGTG CCAGGAGCTG TTGGCGGCGT GAGCCCAGAT 
CATGCAGAGC CAGAGGTTCA GGTGGTGCCT GGGTCCGGCC AGATCATCTT CCTGCCCTTC 
ACTTGCATTG GCTACACAGC CACCAACCAG GACTTTATCC AGCGCCTCAG CACACTGATC 
CGCCAGGCCA TTGAACGGCA ACTCCCTGCC TGGATTGAGG CTGCCAACCA GCGCGAGGAA 
GCCCATGGTG AGCAGGGCGA GGAGGAAGAA GAGGAAGAGG AAGAGGAGGA TGTTGCTGAG 
AACCGCTACT TTGAAATGGG ACCCCCAGAC GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA 
GAAGAGGATG AGGAAGACGA GGACGAGGAG GCGGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCCCTGGGCG CCGATGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA AGGCAGCATC CGCCAGTTCG CTGCCTGCCT TGTGCTCACT 
GACTTCGGCA TCGCAGTCTT TGAGATCCCA CACCAAGAGT CAAGAGGCAG CAGCCAGCAC 
ATCCTCTCAT CCCTGCGTTT TGTCTTCTGC TTCCCACATG GCGACCTCAC GGAGTTTGGC 
TTCCTCATGC CCGAGCTGTG TCTGGTGCTC AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCGG ATGCTGCCAA CTTACACGAG TTCCATGCTG ATCTACGCTC CTGCTTTGCA 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC ATCCTCTACG GCAGCCACAC CACCCTGCAG 
GAGTTCTTGC GCCAGCTGCT CACCTTCTAC AAGGTGGCCG GGGGCTCTCA GGAGCGCAGC 
CAGGGCTGCT TCCCTGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTCCA GACCCCTGCC 
GGGGACTATT CAGGCAATAT CGAATGGGCC AGCTGCACGC TGTGCTCGGC GGTGCGGCGC 
TCCTGCTGCG CGCCCTCGGA GGCCGTCAAG TCCGCTGCCA TCCCCTACTG GCTGCTGCTC 
ACATCCCAGC ATCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA TCGAGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTTAGCCGCG TCCCGCTCTC TACCGTGCTG 
CTAGACCCCA CTCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCTGATGG CCATGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTTACC GCCATCTTTG TGCTGCCCCA CGAGAAATTC 
CACTTCCTGC GAGTCTACAA TCAGCTACGC GCCTCACTGC AGGACCTGAA GACTGTGGTC 
ATCTCCAAGA ATCCTTCAGC TAAGCCAAGA AATCAGCCTG CCAAGAGCAG GGCCAGTGCT 
GAGCAGCGGC TACAGGAGAC CCCAGCAGAC GCTCCGGCTC CAGCTGCAGT CCCACCAACA 
GCTTCAGCTC CAGCCCCCGC AGAGGCCTTG GCTCCAGATC TGGCTCCTGT ACAGGCCCCA 
GGAGAGGACC GAGGTCTAAC TTCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCGGCCCCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCGCAGCAG AGGCTCCAGC TGCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCTTCA 
GCAGAGGCTC CAGCTCCAAA CCAGGCTCCT GCTCCAGCAA GGGGTCCCGC TCCAGCAAGG 
GGTCCCGCTC CAGCAGGGGG TCCCGCTCCA GCAGAGGCCC TGGCACAAGC AGAAGTCCCT 
GCCCAGTACC CAAGTGAGCG CCTAATCCAG TCCACATCTG AAGAGAATCA GATCCCTTCT 
CACTTGCCAG TATGCCCATC ACTCCAGCAC ATTGCCCGTC TTCGGGGGAG AGCCATCATT 
GACCTCTTCC ACAACAGCAT TGCTGAGGTT GAAAATGAGG AGCTGAGGCA CCTCCTGTGG 
TCATCAGTGG TGTTCTACCA GACCCCGGGG CTAGAAGTGA CCGCCTGTGT GCTGCTCTCT 
AGCAAGGCTG TGTACTTCAT ACTGCATGAT GGTCTCCGCC GCTACTTCTC TGAACCACTG 
CAGGATTTCT GGCACCAGAA AAACACCGAC TATAACAACA GTCCTTTCCA CGTCTCTCAG 
TGCTTTGTGT TGAAACTCAG TGACCTGCAG TCAGTCAACG TCGGCCTTTT CGACCAGTAC 
TTCCGGCTGA CGGGCTCCTC CCCGACGCAG GTGGTCACGT GCTTGACTCG CGACAGCTAC 
CTGACGCACT GCTTCCTCCA GCATCTGATG CTTGTGCTGT CCTCCCTGGA GCGCACACCC 
TCGCCTGAGC CTGTTGACAA GGACTTCTAC TCAGAATTTG GGGACAAGAA TACAGGGAAA 
ATGGAGAACT ATGAGCTGAT CCATTCCAGC CGCGTCAAGT TCACCTACCC CAGTGAGGAA 
GAGGTTGGGG ACCTGACCTA TATTGTCGCA CAGAAGATGG CTGATCCTGC AAAGAATCCA 
GCCCTCAGCA TCTTACTGTA CATCCAGGCC TTCCAGGTGG TCACACCACA CCTTGGGCGG 
GGCAGGGGCC CACTGCGCCC TAAGACGCTG CTCCTGACCA GCGCCGAGAT CTTCCTCCTG 
GATGAGGACT ACATCCACTA TCCATTGCCT GAATTTGCCA AAGAGCCACC GCAGAGGGAC 
AGATACCGGC TAGACGATGG CCGCCGGGTC CGGGATTTGG ACCGGGTGCT CATGGGCTAC 
TATCCCTACC CACAGGCCCT CACTCTTGTT TTTGATGACA CACAGGGTCA CGACCTCATG 
GGGAGTGTCA CCCTGGACCA CTTTGGGGAG ATGCCAGGTG GTCCTGGCAG GGTTGGCCAG 
GGCCGAGAGG TACAGTGGCA GGTGTTTGTC CCCAGTGCCG AGAGCCGAGA AAAGCTCATC 
TCACTGCTCG CACGACAGTG GGAAGCTCTT TGTGGCAGGG AGCTGCCTGT GGAGCTCACT 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_006519353.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519416.1 Mus musculus nischarin (Nisch), transcript variant X2, mRNA.

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CloneID	OMu50222
Clone ID Related Accession (Same CDS sequence)	XM_017316115.1 , XM_017316115.2 , XM_006519353.2 , XM_017316116.1
Accession Version	XM_006519353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006519353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTAC AGGAAGAGGC CCTGGCCAGC AGCCTCTCAT CCACGGATAG TCTGCCACCC 
GAGGACCACC GGCCCATTGC CCGAGCCTGC TCTGACTCCT TGGAATCTAT CCCTGCAGGA 
CAGGTGGCCT CTGATGATCT GAGGGATGTG CCAGGAGCTG TTGGCGGCGT GAGCCCAGAT 
CATGCAGAGC CAGAGGTTCA GGTGGTGCCT GGGTCCGGCC AGATCATCTT CCTGCCCTTC 
ACTTGCATTG GCTACACAGC CACCAACCAG GACTTTATCC AGCGCCTCAG CACACTGATC 
CGCCAGGCCA TTGAACGGCA ACTCCCTGCC TGGATTGAGG CTGCCAACCA GCGCGAGGAA 
GCCCATGGTG AGCAGGGCGA GGAGGAAGAA GAGGAAGAGG AAGAGGAGGA TGTTGCTGAG 
AACCGCTACT TTGAAATGGG ACCCCCAGAC GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA 
GAAGAGGATG AGGAAGACGA GGACGAGGAG GCGGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCCCTGGGCG CCGATGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA AGGCAGCATC CGCCAGTTCG CTGCCTGCCT TGTGCTCACT 
GACTTCGGCA TCGCAGTCTT TGAGATCCCA CACCAAGAGT CAAGAGGCAG CAGCCAGCAC 
ATCCTCTCAT CCCTGCGTTT TGTCTTCTGC TTCCCACATG GCGACCTCAC GGAGTTTGGC 
TTCCTCATGC CCGAGCTGTG TCTGGTGCTC AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCGG ATGCTGCCAA CTTACACGAG TTCCATGCTG ATCTACGCTC CTGCTTTGCA 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC ATCCTCTACG GCAGCCACAC CACCCTGCAG 
GAGTTCTTGC GCCAGCTGCT CACCTTCTAC AAGGTGGCCG GGGGCTCTCA GGAGCGCAGC 
CAGGGCTGCT TCCCTGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTCCA GACCCCTGCC 
GGGGACTATT CAGGCAATAT CGAATGGGCC AGCTGCACGC TGTGCTCGGC GGTGCGGCGC 
TCCTGCTGCG CGCCCTCGGA GGCCGTCAAG TCCGCTGCCA TCCCCTACTG GCTGCTGCTC 
ACATCCCAGC ATCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA TCGAGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTTAGCCGCG TCCCGCTCTC TACCGTGCTG 
CTAGACCCCA CTCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCTGATGG CCATGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTTACC GCCATCTTTG TGCTGCCCCA CGAGAAATTC 
CACTTCCTGC GAGTCTACAA TCAGCTACGC GCCTCACTGC AGGACCTGAA GACTGTGGTC 
ATCTCCAAGA ATCCTTCAGC TAAGCCAAGA AATCAGCCTG CCAAGAGCAG GGCCAGTGCT 
GAGCAGCGGC TACAGGAGAC CCCAGCAGAC GCTCCGGCTC CAGCTGCAGT CCCACCAACA 
GCTTCAGCTC CAGCCCCCGC AGAGGCCTTG GCTCCAGATC TGGCTCCTGT ACAGGCCCCA 
GGAGAGGACC GAGGTCTAAC TTCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCGGCCCCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCGCAGCAG AGGCTCCAGC TGCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCTTCA 
GCAGAGGCTC CAGCTCCAAA CCAGGCTCCT GCTCCAGCAA GGGGTCCCGC TCCAGCAAGG 
GGTCCCGCTC CAGCAGGGGG TCCCGCTCCA GCAGAGGCCC TGGCACAAGC AGAAGTCCCT 
GCCCAGTACC CAAGTGAGCG CCTAATCCAG TCCACATCTG AAGAGAATCA GATCCCTTCT 
CACTTGCCAG TATGCCCATC ACTCCAGCAC ATTGCCCGTC TTCGGGGGAG AGCCATCATT 
GACCTCTTCC ACAACAGCAT TGCTGAGGTT GAAAATGAGG AGCTGAGGCA CCTCCTGTGG 
TCATCAGTGG TGTTCTACCA GACCCCGGGG CTAGAAGTGA CCGCCTGTGT GCTGCTCTCT 
AGCAAGGCTG TGTACTTCAT ACTGCATGAT GGTCTCCGCC GCTACTTCTC TGAACCACTG 
CAGGATTTCT GGCACCAGAA AAACACCGAC TATAACAACA GTCCTTTCCA CGTCTCTCAG 
TGCTTTGTGT TGAAACTCAG TGACCTGCAG TCAGTCAACG TCGGCCTTTT CGACCAGTAC 
TTCCGGCTGA CGGGCTCCTC CCCGACGCAG GTGGTCACGT GCTTGACTCG CGACAGCTAC 
CTGACGCACT GCTTCCTCCA GCATCTGATG CTTGTGCTGT CCTCCCTGGA GCGCACACCC 
TCGCCTGAGC CTGTTGACAA GGACTTCTAC TCAGAATTTG GGGACAAGAA TACAGGGAAA 
ATGGAGAACT ATGAGCTGAT CCATTCCAGC CGCGTCAAGT TCACCTACCC CAGTGAGGAA 
GAGGTTGGGG ACCTGACCTA TATTGTCGCA CAGAAGATGG CTGATCCTGC AAAGAATCCA 
GCCCTCAGCA TCTTACTGTA CATCCAGGCC TTCCAGGTGG TCACACCACA CCTTGGGCGG 
GGCAGGGGCC CACTGCGCCC TAAGACGCTG CTCCTGACCA GCGCCGAGAT CTTCCTCCTG 
GATGAGGACT ACATCCACTA TCCATTGCCT GAATTTGCCA AAGAGCCACC GCAGAGGGAC 
AGATACCGGC TAGACGATGG CCGCCGGGTC CGGGATTTGG ACCGGGTGCT CATGGGCTAC 
TATCCCTACC CACAGGCCCT CACTCTTGTT TTTGATGACA CACAGGGTCA CGACCTCATG 
GGGAGTGTCA CCCTGGACCA CTTTGGGGAG ATGCCAGGTG GTCCTGGCAG GGTTGGCCAG 
GGCCGAGAGG TACAGTGGCA GGTGTTTGTC CCCAGTGCCG AGAGCCGAGA AAAGCTCATC 
TCACTGCTCG CACGACAGTG GGAAGCTCTT TGTGGCAGGG AGCTGCCTGT GGAGCTCACT 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519416.1
CDS	3999..7244
Translation	MFVQEEALASSLSSTDSLPPEDHRPIARACSDSLESIPAGQVASDDLRDVPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEEDVAENRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTTLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTPAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVISKNPSAKPRNQPAKSRASAEQRLQETPADAPAPAAVPPTASAPAPAEALAPDLAPVQAPGEDRGLTSAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAPAEAPAAAEAPAAAEAPAAAEAPAAAEAPASAEAPAPNQAPAPARGPAPARGPAPAGGPAPAEALAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHNSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSILLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRVGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_006519353.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nischarin (Nisch), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519353.2

ORF Insert Sequence:

ATGTTTGTAC AGGAAGAGGC CCTGGCCAGC AGCCTCTCAT CCACGGATAG TCTGCCACCC 
GAGGACCACC GGCCCATTGC CCGAGCCTGC TCTGACTCCT TGGAATCTAT CCCTGCAGGA 
CAGGTGGCCT CTGATGATCT GAGGGATGTG CCAGGAGCTG TTGGCGGCGT GAGCCCAGAT 
CATGCAGAGC CAGAGGTTCA GGTGGTGCCT GGGTCCGGCC AGATCATCTT CCTGCCCTTC 
ACTTGCATTG GCTACACAGC CACCAACCAG GACTTTATCC AGCGCCTCAG CACACTGATC 
CGCCAGGCCA TTGAACGGCA ACTCCCTGCC TGGATTGAGG CTGCCAACCA GCGCGAGGAA 
GCCCATGGTG AGCAGGGCGA GGAGGAAGAA GAGGAAGAGG AAGAGGAGGA TGTTGCTGAG 
AACCGCTACT TTGAAATGGG ACCCCCAGAC GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA 
GAAGAGGATG AGGAAGACGA GGACGAGGAG GCGGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCCCTGGGCG CCGATGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA AGGCAGCATC CGCCAGTTCG CTGCCTGCCT TGTGCTCACT 
GACTTCGGCA TCGCAGTCTT TGAGATCCCA CACCAAGAGT CAAGAGGCAG CAGCCAGCAC 
ATCCTCTCAT CCCTGCGTTT TGTCTTCTGC TTCCCACATG GCGACCTCAC GGAGTTTGGC 
TTCCTCATGC CCGAGCTGTG TCTGGTGCTC AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCGG ATGCTGCCAA CTTACACGAG TTCCATGCTG ATCTACGCTC CTGCTTTGCA 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC ATCCTCTACG GCAGCCACAC CACCCTGCAG 
GAGTTCTTGC GCCAGCTGCT CACCTTCTAC AAGGTGGCCG GGGGCTCTCA GGAGCGCAGC 
CAGGGCTGCT TCCCTGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTCCA GACCCCTGCC 
GGGGACTATT CAGGCAATAT CGAATGGGCC AGCTGCACGC TGTGCTCGGC GGTGCGGCGC 
TCCTGCTGCG CGCCCTCGGA GGCCGTCAAG TCCGCTGCCA TCCCCTACTG GCTGCTGCTC 
ACATCCCAGC ATCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA TCGAGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTTAGCCGCG TCCCGCTCTC TACCGTGCTG 
CTAGACCCCA CTCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCTGATGG CCATGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTTACC GCCATCTTTG TGCTGCCCCA CGAGAAATTC 
CACTTCCTGC GAGTCTACAA TCAGCTACGC GCCTCACTGC AGGACCTGAA GACTGTGGTC 
ATCTCCAAGA ATCCTTCAGC TAAGCCAAGA AATCAGCCTG CCAAGAGCAG GGCCAGTGCT 
GAGCAGCGGC TACAGGAGAC CCCAGCAGAC GCTCCGGCTC CAGCTGCAGT CCCACCAACA 
GCTTCAGCTC CAGCCCCCGC AGAGGCCTTG GCTCCAGATC TGGCTCCTGT ACAGGCCCCA 
GGAGAGGACC GAGGTCTAAC TTCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCGGCCCCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCGCAGCAG AGGCTCCAGC TGCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCTTCA 
GCAGAGGCTC CAGCTCCAAA CCAGGCTCCT GCTCCAGCAA GGGGTCCCGC TCCAGCAAGG 
GGTCCCGCTC CAGCAGGGGG TCCCGCTCCA GCAGAGGCCC TGGCACAAGC AGAAGTCCCT 
GCCCAGTACC CAAGTGAGCG CCTAATCCAG TCCACATCTG AAGAGAATCA GATCCCTTCT 
CACTTGCCAG TATGCCCATC ACTCCAGCAC ATTGCCCGTC TTCGGGGGAG AGCCATCATT 
GACCTCTTCC ACAACAGCAT TGCTGAGGTT GAAAATGAGG AGCTGAGGCA CCTCCTGTGG 
TCATCAGTGG TGTTCTACCA GACCCCGGGG CTAGAAGTGA CCGCCTGTGT GCTGCTCTCT 
AGCAAGGCTG TGTACTTCAT ACTGCATGAT GGTCTCCGCC GCTACTTCTC TGAACCACTG 
CAGGATTTCT GGCACCAGAA AAACACCGAC TATAACAACA GTCCTTTCCA CGTCTCTCAG 
TGCTTTGTGT TGAAACTCAG TGACCTGCAG TCAGTCAACG TCGGCCTTTT CGACCAGTAC 
TTCCGGCTGA CGGGCTCCTC CCCGACGCAG GTGGTCACGT GCTTGACTCG CGACAGCTAC 
CTGACGCACT GCTTCCTCCA GCATCTGATG CTTGTGCTGT CCTCCCTGGA GCGCACACCC 
TCGCCTGAGC CTGTTGACAA GGACTTCTAC TCAGAATTTG GGGACAAGAA TACAGGGAAA 
ATGGAGAACT ATGAGCTGAT CCATTCCAGC CGCGTCAAGT TCACCTACCC CAGTGAGGAA 
GAGGTTGGGG ACCTGACCTA TATTGTCGCA CAGAAGATGG CTGATCCTGC AAAGAATCCA 
GCCCTCAGCA TCTTACTGTA CATCCAGGCC TTCCAGGTGG TCACACCACA CCTTGGGCGG 
GGCAGGGGCC CACTGCGCCC TAAGACGCTG CTCCTGACCA GCGCCGAGAT CTTCCTCCTG 
GATGAGGACT ACATCCACTA TCCATTGCCT GAATTTGCCA AAGAGCCACC GCAGAGGGAC 
AGATACCGGC TAGACGATGG CCGCCGGGTC CGGGATTTGG ACCGGGTGCT CATGGGCTAC 
TATCCCTACC CACAGGCCCT CACTCTTGTT TTTGATGACA CACAGGGTCA CGACCTCATG 
GGGAGTGTCA CCCTGGACCA CTTTGGGGAG ATGCCAGGTG GTCCTGGCAG GGTTGGCCAG 
GGCCGAGAGG TACAGTGGCA GGTGTTTGTC CCCAGTGCCG AGAGCCGAGA AAAGCTCATC 
TCACTGCTCG CACGACAGTG GGAAGCTCTT TGTGGCAGGG AGCTGCCTGT GGAGCTCACT 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nisch ( XM_017316116.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171605.1 Mus musculus nischarin (Nisch), transcript variant X2, mRNA.

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CloneID	OMu50222
Clone ID Related Accession (Same CDS sequence)	XM_017316115.1 , XM_017316115.2 , XM_006519353.2 , XM_017316116.1
Accession Version	XM_017316116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nischarin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTAC AGGAAGAGGC CCTGGCCAGC AGCCTCTCAT CCACGGATAG TCTGCCACCC 
GAGGACCACC GGCCCATTGC CCGAGCCTGC TCTGACTCCT TGGAATCTAT CCCTGCAGGA 
CAGGTGGCCT CTGATGATCT GAGGGATGTG CCAGGAGCTG TTGGCGGCGT GAGCCCAGAT 
CATGCAGAGC CAGAGGTTCA GGTGGTGCCT GGGTCCGGCC AGATCATCTT CCTGCCCTTC 
ACTTGCATTG GCTACACAGC CACCAACCAG GACTTTATCC AGCGCCTCAG CACACTGATC 
CGCCAGGCCA TTGAACGGCA ACTCCCTGCC TGGATTGAGG CTGCCAACCA GCGCGAGGAA 
GCCCATGGTG AGCAGGGCGA GGAGGAAGAA GAGGAAGAGG AAGAGGAGGA TGTTGCTGAG 
AACCGCTACT TTGAAATGGG ACCCCCAGAC GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA 
GAAGAGGATG AGGAAGACGA GGACGAGGAG GCGGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCCCTGGGCG CCGATGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA AGGCAGCATC CGCCAGTTCG CTGCCTGCCT TGTGCTCACT 
GACTTCGGCA TCGCAGTCTT TGAGATCCCA CACCAAGAGT CAAGAGGCAG CAGCCAGCAC 
ATCCTCTCAT CCCTGCGTTT TGTCTTCTGC TTCCCACATG GCGACCTCAC GGAGTTTGGC 
TTCCTCATGC CCGAGCTGTG TCTGGTGCTC AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCGG ATGCTGCCAA CTTACACGAG TTCCATGCTG ATCTACGCTC CTGCTTTGCA 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC ATCCTCTACG GCAGCCACAC CACCCTGCAG 
GAGTTCTTGC GCCAGCTGCT CACCTTCTAC AAGGTGGCCG GGGGCTCTCA GGAGCGCAGC 
CAGGGCTGCT TCCCTGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTCCA GACCCCTGCC 
GGGGACTATT CAGGCAATAT CGAATGGGCC AGCTGCACGC TGTGCTCGGC GGTGCGGCGC 
TCCTGCTGCG CGCCCTCGGA GGCCGTCAAG TCCGCTGCCA TCCCCTACTG GCTGCTGCTC 
ACATCCCAGC ATCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA TCGAGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTTAGCCGCG TCCCGCTCTC TACCGTGCTG 
CTAGACCCCA CTCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCTGATGG CCATGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTTACC GCCATCTTTG TGCTGCCCCA CGAGAAATTC 
CACTTCCTGC GAGTCTACAA TCAGCTACGC GCCTCACTGC AGGACCTGAA GACTGTGGTC 
ATCTCCAAGA ATCCTTCAGC TAAGCCAAGA AATCAGCCTG CCAAGAGCAG GGCCAGTGCT 
GAGCAGCGGC TACAGGAGAC CCCAGCAGAC GCTCCGGCTC CAGCTGCAGT CCCACCAACA 
GCTTCAGCTC CAGCCCCCGC AGAGGCCTTG GCTCCAGATC TGGCTCCTGT ACAGGCCCCA 
GGAGAGGACC GAGGTCTAAC TTCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCGGCCCCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCGCAGCAG AGGCTCCAGC TGCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCTTCA 
GCAGAGGCTC CAGCTCCAAA CCAGGCTCCT GCTCCAGCAA GGGGTCCCGC TCCAGCAAGG 
GGTCCCGCTC CAGCAGGGGG TCCCGCTCCA GCAGAGGCCC TGGCACAAGC AGAAGTCCCT 
GCCCAGTACC CAAGTGAGCG CCTAATCCAG TCCACATCTG AAGAGAATCA GATCCCTTCT 
CACTTGCCAG TATGCCCATC ACTCCAGCAC ATTGCCCGTC TTCGGGGGAG AGCCATCATT 
GACCTCTTCC ACAACAGCAT TGCTGAGGTT GAAAATGAGG AGCTGAGGCA CCTCCTGTGG 
TCATCAGTGG TGTTCTACCA GACCCCGGGG CTAGAAGTGA CCGCCTGTGT GCTGCTCTCT 
AGCAAGGCTG TGTACTTCAT ACTGCATGAT GGTCTCCGCC GCTACTTCTC TGAACCACTG 
CAGGATTTCT GGCACCAGAA AAACACCGAC TATAACAACA GTCCTTTCCA CGTCTCTCAG 
TGCTTTGTGT TGAAACTCAG TGACCTGCAG TCAGTCAACG TCGGCCTTTT CGACCAGTAC 
TTCCGGCTGA CGGGCTCCTC CCCGACGCAG GTGGTCACGT GCTTGACTCG CGACAGCTAC 
CTGACGCACT GCTTCCTCCA GCATCTGATG CTTGTGCTGT CCTCCCTGGA GCGCACACCC 
TCGCCTGAGC CTGTTGACAA GGACTTCTAC TCAGAATTTG GGGACAAGAA TACAGGGAAA 
ATGGAGAACT ATGAGCTGAT CCATTCCAGC CGCGTCAAGT TCACCTACCC CAGTGAGGAA 
GAGGTTGGGG ACCTGACCTA TATTGTCGCA CAGAAGATGG CTGATCCTGC AAAGAATCCA 
GCCCTCAGCA TCTTACTGTA CATCCAGGCC TTCCAGGTGG TCACACCACA CCTTGGGCGG 
GGCAGGGGCC CACTGCGCCC TAAGACGCTG CTCCTGACCA GCGCCGAGAT CTTCCTCCTG 
GATGAGGACT ACATCCACTA TCCATTGCCT GAATTTGCCA AAGAGCCACC GCAGAGGGAC 
AGATACCGGC TAGACGATGG CCGCCGGGTC CGGGATTTGG ACCGGGTGCT CATGGGCTAC 
TATCCCTACC CACAGGCCCT CACTCTTGTT TTTGATGACA CACAGGGTCA CGACCTCATG 
GGGAGTGTCA CCCTGGACCA CTTTGGGGAG ATGCCAGGTG GTCCTGGCAG GGTTGGCCAG 
GGCCGAGAGG TACAGTGGCA GGTGTTTGTC CCCAGTGCCG AGAGCCGAGA AAAGCTCATC 
TCACTGCTCG CACGACAGTG GGAAGCTCTT TGTGGCAGGG AGCTGCCTGT GGAGCTCACT 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171605.1
CDS	2721..5966
Translation	MFVQEEALASSLSSTDSLPPEDHRPIARACSDSLESIPAGQVASDDLRDVPGAVGGVSPDHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREEAHGEQGEEEEEEEEEEDVAENRYFEMGPPDAEEEEGSGQGEEDEEDEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTTLQEFLRQLLTFYKVAGGSQERSQGCFPVYLVYSDKRMVQTPAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTSQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVISKNPSAKPRNQPAKSRASAEQRLQETPADAPAPAAVPPTASAPAPAEALAPDLAPVQAPGEDRGLTSAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAAAEAPAPAEAPAAAEAPAAAEAPAAAEAPAAAEAPASAEAPAPNQAPAPARGPAPARGPAPAGGPAPAEALAQAEVPAQYPSERLIQSTSEENQIPSHLPVCPSLQHIARLRGRAIIDLFHNSIAEVENEELRHLLWSSVVFYQTPGLEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSILLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRVGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

Target ORF information:

RefSeq Version	XM_017316116.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nischarin (Nisch), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316116.1

ORF Insert Sequence:

ATGTTTGTAC AGGAAGAGGC CCTGGCCAGC AGCCTCTCAT CCACGGATAG TCTGCCACCC 
GAGGACCACC GGCCCATTGC CCGAGCCTGC TCTGACTCCT TGGAATCTAT CCCTGCAGGA 
CAGGTGGCCT CTGATGATCT GAGGGATGTG CCAGGAGCTG TTGGCGGCGT GAGCCCAGAT 
CATGCAGAGC CAGAGGTTCA GGTGGTGCCT GGGTCCGGCC AGATCATCTT CCTGCCCTTC 
ACTTGCATTG GCTACACAGC CACCAACCAG GACTTTATCC AGCGCCTCAG CACACTGATC 
CGCCAGGCCA TTGAACGGCA ACTCCCTGCC TGGATTGAGG CTGCCAACCA GCGCGAGGAA 
GCCCATGGTG AGCAGGGCGA GGAGGAAGAA GAGGAAGAGG AAGAGGAGGA TGTTGCTGAG 
AACCGCTACT TTGAAATGGG ACCCCCAGAC GCAGAGGAAG AGGAGGGGAG TGGCCAGGGA 
GAAGAGGATG AGGAAGACGA GGACGAGGAG GCGGAGGAGG AGCGCCTGGC TCTGGAGTGG 
GCCCTGGGCG CCGATGAGGA CTTCCTGCTG GAGCACATCC GCATCCTCAA GGTGCTCTGG 
TGCTTCCTGA TCCACGTGCA AGGCAGCATC CGCCAGTTCG CTGCCTGCCT TGTGCTCACT 
GACTTCGGCA TCGCAGTCTT TGAGATCCCA CACCAAGAGT CAAGAGGCAG CAGCCAGCAC 
ATCCTCTCAT CCCTGCGTTT TGTCTTCTGC TTCCCACATG GCGACCTCAC GGAGTTTGGC 
TTCCTCATGC CCGAGCTGTG TCTGGTGCTC AAGGTGCGGC ACAGTGAGAA CACGCTCTTC 
ATCATCTCGG ATGCTGCCAA CTTACACGAG TTCCATGCTG ATCTACGCTC CTGCTTTGCA 
CCGCAGCACA TGGCCATGCT GTGCAGCCCC ATCCTCTACG GCAGCCACAC CACCCTGCAG 
GAGTTCTTGC GCCAGCTGCT CACCTTCTAC AAGGTGGCCG GGGGCTCTCA GGAGCGCAGC 
CAGGGCTGCT TCCCTGTCTA CCTGGTCTAC AGTGACAAGC GCATGGTCCA GACCCCTGCC 
GGGGACTATT CAGGCAATAT CGAATGGGCC AGCTGCACGC TGTGCTCGGC GGTGCGGCGC 
TCCTGCTGCG CGCCCTCGGA GGCCGTCAAG TCCGCTGCCA TCCCCTACTG GCTGCTGCTC 
ACATCCCAGC ATCTCAACGT CATCAAGGCC GACTTCAACC CCATGCCCAA TCGAGGCACC 
CACAACTGCC GCAACCGCAA CAGCTTCAAG CTTAGCCGCG TCCCGCTCTC TACCGTGCTG 
CTAGACCCCA CTCGCAGCTG CACCCAGCCA CGGGGTGCCT TCGCTGATGG CCATGTGCTC 
GAGCTGCTCG TGGGCTACCG CTTCGTTACC GCCATCTTTG TGCTGCCCCA CGAGAAATTC 
CACTTCCTGC GAGTCTACAA TCAGCTACGC GCCTCACTGC AGGACCTGAA GACTGTGGTC 
ATCTCCAAGA ATCCTTCAGC TAAGCCAAGA AATCAGCCTG CCAAGAGCAG GGCCAGTGCT 
GAGCAGCGGC TACAGGAGAC CCCAGCAGAC GCTCCGGCTC CAGCTGCAGT CCCACCAACA 
GCTTCAGCTC CAGCCCCCGC AGAGGCCTTG GCTCCAGATC TGGCTCCTGT ACAGGCCCCA 
GGAGAGGACC GAGGTCTAAC TTCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCCGCA 
GCAGAGGCTC CAGCCGCAGC AGAGGCTCCA GCCGCAGCAG AGGCTCCAGC CGCAGCAGAG 
GCTCCAGCCG CAGCAGAGGC TCCGGCCCCA GCAGAGGCTC CAGCCGCAGC AGAGGCTCCG 
GCCGCAGCAG AGGCTCCAGC TGCAGCAGAG GCTCCAGCCG CAGCAGAGGC TCCAGCTTCA 
GCAGAGGCTC CAGCTCCAAA CCAGGCTCCT GCTCCAGCAA GGGGTCCCGC TCCAGCAAGG 
GGTCCCGCTC CAGCAGGGGG TCCCGCTCCA GCAGAGGCCC TGGCACAAGC AGAAGTCCCT 
GCCCAGTACC CAAGTGAGCG CCTAATCCAG TCCACATCTG AAGAGAATCA GATCCCTTCT 
CACTTGCCAG TATGCCCATC ACTCCAGCAC ATTGCCCGTC TTCGGGGGAG AGCCATCATT 
GACCTCTTCC ACAACAGCAT TGCTGAGGTT GAAAATGAGG AGCTGAGGCA CCTCCTGTGG 
TCATCAGTGG TGTTCTACCA GACCCCGGGG CTAGAAGTGA CCGCCTGTGT GCTGCTCTCT 
AGCAAGGCTG TGTACTTCAT ACTGCATGAT GGTCTCCGCC GCTACTTCTC TGAACCACTG 
CAGGATTTCT GGCACCAGAA AAACACCGAC TATAACAACA GTCCTTTCCA CGTCTCTCAG 
TGCTTTGTGT TGAAACTCAG TGACCTGCAG TCAGTCAACG TCGGCCTTTT CGACCAGTAC 
TTCCGGCTGA CGGGCTCCTC CCCGACGCAG GTGGTCACGT GCTTGACTCG CGACAGCTAC 
CTGACGCACT GCTTCCTCCA GCATCTGATG CTTGTGCTGT CCTCCCTGGA GCGCACACCC 
TCGCCTGAGC CTGTTGACAA GGACTTCTAC TCAGAATTTG GGGACAAGAA TACAGGGAAA 
ATGGAGAACT ATGAGCTGAT CCATTCCAGC CGCGTCAAGT TCACCTACCC CAGTGAGGAA 
GAGGTTGGGG ACCTGACCTA TATTGTCGCA CAGAAGATGG CTGATCCTGC AAAGAATCCA 
GCCCTCAGCA TCTTACTGTA CATCCAGGCC TTCCAGGTGG TCACACCACA CCTTGGGCGG 
GGCAGGGGCC CACTGCGCCC TAAGACGCTG CTCCTGACCA GCGCCGAGAT CTTCCTCCTG 
GATGAGGACT ACATCCACTA TCCATTGCCT GAATTTGCCA AAGAGCCACC GCAGAGGGAC 
AGATACCGGC TAGACGATGG CCGCCGGGTC CGGGATTTGG ACCGGGTGCT CATGGGCTAC 
TATCCCTACC CACAGGCCCT CACTCTTGTT TTTGATGACA CACAGGGTCA CGACCTCATG 
GGGAGTGTCA CCCTGGACCA CTTTGGGGAG ATGCCAGGTG GTCCTGGCAG GGTTGGCCAG 
GGCCGAGAGG TACAGTGGCA GGTGTTTGTC CCCAGTGCCG AGAGCCGAGA AAAGCTCATC 
TCACTGCTCG CACGACAGTG GGAAGCTCTT TGTGGCAGGG AGCTGCCTGT GGAGCTCACT 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.
Nature neuroscience(2018 Jan)
Gstrein T,Edwards A,P?istoupilov? A,Leca I,Breuss M,Pilat-Carotta S,Hansen AH,Tripathy R,Traunbauer AK,Hochstoeger T,Rosoklija G,Repic M,Landler L,Str?neck? V,D?rnberger G,Keane TM,Zuber J,Adams DJ,Flint J,Honzik T,Gut M,Beltran S,Mechtler K,Sherr E,Kmoch S,Gut I,Keays DA

Nischarin inhibition alters energy metabolism by activating AMP-activated protein kinase.
The Journal of biological chemistry292(41)16833-16846(2017 10)
Dong S,Baranwal S,Garcia A,Serrano-Gomez SJ,Eastlack S,Iwakuma T,Mercante D,Mauvais-Jarvis F,Alahari SK

A mutation in Nischarin causes otitis media via LIMK1 and NF-?B pathways.
PLoS genetics13(8)e1006969(2017 Aug)
Crompton M,Purnell T,Tyrer HE,Parker A,Ball G,Hardisty-Hughes RE,Gale R,Williams D,Dean CH,Simon MM,Mallon AM,Wells S,Bhutta MF,Burton MJ,Tateossian H,Brown SDM

Inhibition of Nischarin Expression Promotes Neurite Outgrowth through Regulation of PAK Activity.
PloS one10(12)e0144948(2015)
Ding Y,Li Y,Lu L,Zhang R,Zeng L,Wang L,Zhang X

Improvement of obesity by activation of I1-imidazoline receptors in high fat diet-fed mice.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(8)581-5(2013 Aug)
Chung HH,Cheng J

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Nisch cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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