Skint1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Skint1
Entrez Gene ID	639781
Full Name	selection and upkeep of intraepithelial T cells 1
Synonyms	Skint-1
General protein information	Preferred Names selection and upkeep of intraepithelial T cells 1 Names selection and upkeep of intraepithelial T-cells protein 1 Immunoglobulin-like and transmembrane domain-containing protein expressed in skin and thymus protein 1 skint 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102662.1	NP_001096132.1	selection and upkeep of intraepithelial T-cells protein 1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Skint1	NP_001129388.1
Bos taurus (cattle)	SKINT1	NP_001120788.1
Mus musculus (house mouse)	Skint1	NP_001096132.1

Gene Ontology
Bibliography

Related articles in PubMed

Human papillomavirus oncoproteins induce a reorganization of epithelial-associated ?? T cells promoting tumor formation.
Van Hede D, Polese B, Humblet C, Wilharm A, Renoux V, Dortu E, de Leval L, Delvenne P, Desmet CJ, Bureau F, Vermijlen D, Jacobs N
Proceedings of the National Academy of Sciences of the United States of America114(43)E9056-E9065(2017 Oct)

Characterization of a Putative Receptor Binding Surface on Skint-1, a Critical Determinant of Dendritic Epidermal T Cell Selection.
Salim M, Knowles TJ, Hart R, Mohammed F, Woodward MJ, Willcox CR, Overduin M, Hayday AC, Willcox BE
The Journal of biological chemistry291(17)9310-21(2016 Apr)

PLZF Controls the Development of Fetal-Derived IL-17+V?6+ ?? T Cells.
Lu Y, Cao X, Zhang X, Kovalovsky D
Journal of immunology (Baltimore, Md. : 1950)195(9)4273-81(2015 Nov)

The TCR ligand-inducible expression of CD73 marks ?? lineage commitment and a metastable intermediate in effector specification.
Coffey F, Lee SY, Buus TB, Lauritsen JP, Wong GW, Joachims ML, Thompson LF, Z??iga-Pfl?cker JC, Kappes DJ, Wiest DL
The Journal of experimental medicine211(2)329-43(2014 Feb)

Cross-reactivity of anti-programmed death ligand 2 polyclonal antibody in mouse tissues.
Zhao Y, Bian G, Yu C, Liu F, Liu L, Guo H, Guo J, Ju G, Wang J
Science China. Life sciences55(11)940-7(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results provide the first structural insights into the Skint family, identifying a putative receptor binding surface that directly implicates Skint-1 in receptor-ligand interactions crucial for DETC selection.
Title: Characterization of a Putative Receptor Binding Surface on Skint-1, a Critical Determinant of Dendritic Epidermal T Cell Selection.

identifies a common molecular mechanism for the development of interferon-gamma-secreting versus interleukin-17-secreting gammadelta T cells
Title: Skint-1 identifies a common molecular mechanism for the development of interferon-?-secreting versus interleukin-17-secreting ?? T cells.

transgenic Skint-1, across a broad range of transgenic Skint-1, across a broad range of expression levels, precisely and selectively determines the Vgamma5Vdelta1(+) dendritic epidermal T-cell compartment
Title: Skint-1 is a highly specific, unique selecting component for epidermal T cells.

Skint1 is the prototypic member of a rapidly evolving family of at least 11 genes in mouse, with greatest similarity to the butyrophilin genes.
Title: Skint1, the prototype of a newly identified immunoglobulin superfamily gene cluster, positively selects epidermal gammadelta T cells.

The following Skint1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Skint1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03786	NM_001102662.1 Latest version!	Mus musculus selection and upkeep of intraepithelial T cells 1 (Skint1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Skint1 ( NM_001102662.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001096132.1 Mus musculus selection and upkeep of intraepithelial T cells 1 (Skint1), mRNA.

Price & Availability↑

CloneID	OMu03786
Clone ID Related Accession (Same CDS sequence)	NM_001102662.1
Accession Version	NM_001102662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Mus musculus(house mouse)
Product	selection and upkeep of intraepithelial T-cells protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF494889.1. On or before Sep 2, 2007 this sequence version replaced XM_001479153.1, XM_001474878.1. ##Evidence-Data-START## Transcript exon combination :: EF494889.1, EF494890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGGGATCCA CAGGACTATG TTTTTATGGA CACTGTATTG TCATGTTCCT TCTTCAGATG 
GTCACAGCAA GCTCAGAGCC ATTTATAGTG AACGGCTTAG AGGGGCCAGT CTTGGCTTCA 
TTGGGTGGAA ACTTAGAACT CAGTTGTCAG TTGTCTCCAC CACAACAAGC CCAGCACATG 
GAGATTCGCT GGTTCCGGAA CCTCTATACA GAGCCTGTGC ACCTGTACAG AGATGGCAAA 
GACATGTTTG GAGAAATTAT CTCCAAGTAT GTGGAACGTA CAGAACTCTT AAAAGATGGG 
ATTGGAGAAG GGAAAGTGAC CCTTAGGATC TTTAATGTGA CTGTTGATGA TGATGGGTCT 
TACCACTGTG TGTTCAAAGA TGGTGATTTC TATGAAGAGC ACATAACAGA GGTCAAGATC 
ACAGCCATAA ACTTACAGGT ACAGATTCAT GTGCACCCTC CTAATACCAA AGGTGTGATA 
GTGGAGTGTC ACTCTGGAGG TTGGTTCCCA CGACCTCTCA TGCAATGGAG AGACAGAAGA 
GGGGAGGTCA TTCCAGCTGC ATCAAAATCC CACTCACAGG GTAGAGACAA ACTGTTCAAT 
ATGAAGATAT CCCTTCTCAT TAGTGAAAGC TTCTTCCAGA AAGTCATTTG TTGTCTTCAG 
AACCCTCTAA CAGGCCAAGA GGAAAGGACA AGTGTTATTC TATCAGATGC ATTTTTTTCA 
TGGAATAGGA TTTGGAAAAT GATTCTGGGT ATAATACTAT CTATGATGGT GGTCTCTATC 
TTTGTTTTTA GTTGTCTGCT ACATCATGAG CATAAAGTTT GTAAGTGGAA GTGGGATGCT 
CCTTGGATAA AAGGATTATT AATAATGACA TCTTCTATGG TGACTGTTGT ATTAGTCATG 
GTGTATTTAC ATATGAAACA AAGAGTTCCA GTTTCAGATG TACATTTTGA ATTGGATACT 
TTGTGGGTGG AAGACATAAG TGTAATCCTG TGTTCACTGA TGGTTCCTGC CACCATGCTT 
GTTTCCTATA CATATTTCAG GTTGAAAGAC TGGTGCCAGC ACAATCATGC TCAGAGAGTC 
TTCACCAGCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096132.1
CDS	81..1175
Translation	MGSTGLCFYGHCIVMFLLQMVTASSEPFIVNGLEGPVLASLGGNLELSCQLSPPQQAQHMEIRWFRNLYTEPVHLYRDGKDMFGEIISKYVERTELLKDGIGEGKVTLRIFNVTVDDDGSYHCVFKDGDFYEEHITEVKITAINLQVQIHVHPPNTKGVIVECHSGGWFPRPLMQWRDRRGEVIPAASKSHSQGRDKLFNMKISLLISESFFQKVICCLQNPLTGQEERTSVILSDAFFSWNRIWKMILGIILSMMVVSIFVFSCLLHHEHKVCKWKWDAPWIKGLLIMTSSMVTVVLVMVYLHMKQRVPVSDVHFELDTLWVEDISVILCSLMVPATMLVSYTYFRLKDWCQHNHAQRVFTSN

Target ORF information:

RefSeq Version	NM_001102662.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus selection and upkeep of intraepithelial T cells 1 (Skint1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102662.1

ORF Insert Sequence:

ATGGGATCCA CAGGACTATG TTTTTATGGA CACTGTATTG TCATGTTCCT TCTTCAGATG 
GTCACAGCAA GCTCAGAGCC ATTTATAGTG AACGGCTTAG AGGGGCCAGT CTTGGCTTCA 
TTGGGTGGAA ACTTAGAACT CAGTTGTCAG TTGTCTCCAC CACAACAAGC CCAGCACATG 
GAGATTCGCT GGTTCCGGAA CCTCTATACA GAGCCTGTGC ACCTGTACAG AGATGGCAAA 
GACATGTTTG GAGAAATTAT CTCCAAGTAT GTGGAACGTA CAGAACTCTT AAAAGATGGG 
ATTGGAGAAG GGAAAGTGAC CCTTAGGATC TTTAATGTGA CTGTTGATGA TGATGGGTCT 
TACCACTGTG TGTTCAAAGA TGGTGATTTC TATGAAGAGC ACATAACAGA GGTCAAGATC 
ACAGCCATAA ACTTACAGGT ACAGATTCAT GTGCACCCTC CTAATACCAA AGGTGTGATA 
GTGGAGTGTC ACTCTGGAGG TTGGTTCCCA CGACCTCTCA TGCAATGGAG AGACAGAAGA 
GGGGAGGTCA TTCCAGCTGC ATCAAAATCC CACTCACAGG GTAGAGACAA ACTGTTCAAT 
ATGAAGATAT CCCTTCTCAT TAGTGAAAGC TTCTTCCAGA AAGTCATTTG TTGTCTTCAG 
AACCCTCTAA CAGGCCAAGA GGAAAGGACA AGTGTTATTC TATCAGATGC ATTTTTTTCA 
TGGAATAGGA TTTGGAAAAT GATTCTGGGT ATAATACTAT CTATGATGGT GGTCTCTATC 
TTTGTTTTTA GTTGTCTGCT ACATCATGAG CATAAAGTTT GTAAGTGGAA GTGGGATGCT 
CCTTGGATAA AAGGATTATT AATAATGACA TCTTCTATGG TGACTGTTGT ATTAGTCATG 
GTGTATTTAC ATATGAAACA AAGAGTTCCA GTTTCAGATG TACATTTTGA ATTGGATACT 
TTGTGGGTGG AAGACATAAG TGTAATCCTG TGTTCACTGA TGGTTCCTGC CACCATGCTT 
GTTTCCTATA CATATTTCAG GTTGAAAGAC TGGTGCCAGC ACAATCATGC TCAGAGAGTC 
TTCACCAGCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human papillomavirus oncoproteins induce a reorganization of epithelial-associated ?? T cells promoting tumor formation.
Proceedings of the National Academy of Sciences of the United States of America114(43)E9056-E9065(2017 Oct)
Van Hede D,Polese B,Humblet C,Wilharm A,Renoux V,Dortu E,de Leval L,Delvenne P,Desmet CJ,Bureau F,Vermijlen D,Jacobs N

Characterization of a Putative Receptor Binding Surface on Skint-1, a Critical Determinant of Dendritic Epidermal T Cell Selection.
The Journal of biological chemistry291(17)9310-21(2016 Apr)
Salim M,Knowles TJ,Hart R,Mohammed F,Woodward MJ,Willcox CR,Overduin M,Hayday AC,Willcox BE

PLZF Controls the Development of Fetal-Derived IL-17+V?6+ ?? T Cells.
Journal of immunology (Baltimore, Md. : 1950)195(9)4273-81(2015 Nov)
Lu Y,Cao X,Zhang X,Kovalovsky D

The TCR ligand-inducible expression of CD73 marks ?? lineage commitment and a metastable intermediate in effector specification.
The Journal of experimental medicine211(2)329-43(2014 Feb)
Coffey F,Lee SY,Buus TB,Lauritsen JP,Wong GW,Joachims ML,Thompson LF,Z??iga-Pfl?cker JC,Kappes DJ,Wiest DL

Cross-reactivity of anti-programmed death ligand 2 polyclonal antibody in mouse tissues.
Science China. Life sciences55(11)940-7(2012 Nov)
Zhao Y,Bian G,Yu C,Liu F,Liu L,Guo H,Guo J,Ju G,Wang J

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Skint1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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