Ube4b cDNA ORF clone, Mus musculus(house mouse)

The following Ube4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu12578 NM_022022.3
Latest version!
Mus musculus ubiquitination factor E4B (Ube4b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $692.30
$989.00
OMu34371 XM_006539088.4
Latest version!
Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu34372 XM_006539089.3
Latest version!
Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
View Old Accession Versions >>
OMu34371 XM_006539088.3 Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu12578
    Clone ID Related Accession (Same CDS sequence) NM_022022.3
    Accession Version NM_022022.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Mus musculus(house mouse)
    Product ubiquitin conjugation factor E4 B
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK016689.1, BY279585.1 and BC075620.1. On or before Apr 5, 2008 this sequence version replaced XM_001477015.1, NM_022022.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF260926.1, BC067402.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
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    121
    181
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
    CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT
    CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG
    ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA
    GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC
    TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG
    GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG
    AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG
    CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT
    TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT
    CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT
    CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC
    CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG
    GGCTCCTTCA GCACCAGCTC GTTGTCTAGT TTGGGAGCCT CTGGTGGAGC AAGTAACTGG
    GATTCGTACA GTGACCATTT CACCATTGAA ACCTGCAAAG AGACGGACAT GCTGAACTAC
    CTCATCGAGT GTTTTGACCG AGTTGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC
    CAGCCAGCAG TCAGCCAGCT TCTGAGCAAC ATCCGCTCGC AGTGCATCTC CCACACCGCA
    CTAGTGCTAC AAGGTTCCCT CACACAGCCC AGGTCCTTGC AACAGCCATC CTTCCTCGTG
    CCGTACATGC TCTGCAGGAA CCTCCCGTAC GGCTTCATTC AAGAGCTGGT GAGGACCACT
    CACCAGGATG AAGAGGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCGCTG
    GCTGCCAAAG AGTGCTCCTT GGAAAGCGAC TACTTTAAGT ACCCCCTCAT GGCACTGGGT
    GAACTCTGTG AAACCAAGTT TGGGAAGACA CACCCTATGT GCAATTTGGT CGCTTCTTTA
    CCCCTGTGGT TGCCGAAGTC CTTAAGCCCT GGTTCTGGGC GGGAGCTGCA GAGACTCTCC
    TACTTAGGGG CCTTCTTTAG CTTCTCAGTC TTCGCAGAGG ACGATGCAAA GGTGGTTGAG
    AAATACTTCT CAGGGCCTGC CATTACCCTG GAGAACACAC GCGTGGTCAG CCAGTCACTA
    CAGCATTACC TGGAGCTGGG ACGGCAAGAA CTTTTCAAGA TTCTACATAG TATTTTGTTA
    AATGGCGAAA CTCGTGAAGC GGCTCTCAGC TACATGGCGG CCCTTGTCAA TGCCAACATG
    AAGAAAGCCC AGATGCAGGC AGATGATCGG TTGGTGTCTA CAGACGGGTT TATGCTGAAT
    CTCCTTTGGG TCCTGCAGCA GCTAAGTACA AAAATCAAGT TAGAAACAGT TGACCCCACT
    TACATATTCC ACCCAAGATG TCGGATCACT CTCCCGAATG ATGAGACACG GATAAATGCA
    ACGATGGAGG ATGTGAATGA GCGGCTGACA GAGCTCTATG GTGATCAGCC TCCATTTTCT
    GAGCCAAAAT TCCCTACGGA ATGTTTCTTT CTCACCCTGC ATGCTCATCA CCTCTCTATT
    CTGCCTAGTT GTCGTCGCTA CATTCGCCGA CTTCGAGCCA TCCGCGAGCT CAATAGAACC
    GTGGAAGATT TGAAAAATAA TGAAAGCCAG TGGAAAGATT CCCCACTGGC CACCAGACAC
    CGTGAAATGC TGAAGCGCTG TAAAACTCAG CTTAAGAAAC TGGTACGGTG CAAGGCCTGT
    GCTGACGCTG GCTTACTTGA CGAGAGCTTC CTAAGAAGAT GCCTGAATTT TTATGGCCTT
    CTCATTCAGC TGATGCTGCG CATCCTGGAC CCTGCATATC CTGACGTAAC ACTGCCTTTG
    AATTCAGAAG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTCGA AGATGTTGCT
    GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TTTATGAGCC CTGCACTCAG
    GACATTGTCA TGTTCCTCGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAACCCTTAC
    CTAGTGGCCA AGCTGGTGGA GGTCATGTTC ATGACCAACC CTTCTGTCCA GCCGAGAACT
    CAGAAGTTCT TTGAGATGAT TGAGAACCAT CCTCTCTCTA CCAAACTGCT GGTGCCGTCC
    CTCATGAAGT TCTATACAGA TGTCGAGCAT ACTGGAGCCA CCAGCGAGTT CTATGACAAG
    TTCACAATTC GCTATCACAT TAGCACTATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC
    CATGGCACCT TCATGGAGGA GTTCAATTCT GGAAAGCAGT TCGTTCGGTA TATCAATATG
    CTAATAAATG ACACGACCTT TTTACTGGAC GAAAGTCTGG AGTCTCTGAA GCGGATCCAT
    GAAGTGCAAG AAGAGATGAA GAACAAAGAA CAGTGGGACC AGCTGCCTCG GGATCAGCAG
    CAGGCCCGGC AGTCTCAGCT TGCTCAGGAC GAGCGTGTTT CCCGTTCTTA TCTTGCCCTG
    GCAACTGAAA CCGTGGACAT GTTCCATCTC CTCACCAAGC AAGTCCAGAA GCCGTTCCTG
    AGGCCAGAGC TTGGTCCCCG ATTAGCAGCC ATGCTGAACT TTAACCTGCA GCAGCTCTGC
    GGACCCAAGT GCCGGGACCT GAAAGTTGAA AACCCAGAGA AGTATGGTTT TGAGCCAAAG
    AAGCTGTTGG ACCAACTGAC AGATATTTAT TTACAATTGG ACTGTGCTCG CTTTGCTAAA
    GCCATCGCCG ATGACCAGCG ATCCTACAGC AAGGAACTGT TTGAAGAAGT CATTTCAAAG
    ATGCGGAAGG CGGGAATCAA ATCCACCATC GCAATAGAGA AGTTTAAACT TCTTGCAGAG
    AAAGTGGAGG AAATCGTGGC AAAGAATGCG CGGGCAGAAA TAGACTACAG CGATGCCCCG
    GACGAGTTCA GAGACCCTCT GATGGACACC CTGATGACCG ATCCCGTGAG ACTGCCCTCT
    GGCACCGTCA TGGACCGTTC TATCATCCTG CGGCATCTGC TCAACTCCCC CACCGACCCC
    TTCAACCGCC AGATGCTGAC TGAGAGCATG CTGGAGCCAG TGCCAGAGCT AAAGGAGCAG
    ATTCAGGCCT GGATGAGAGA GAAACAGAGC AGTGACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_071305.2
    CDS727..4248
    Translation

    Target ORF information:

    RefSeq Version NM_022022.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus ubiquitination factor E4B (Ube4b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022022.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
    CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT
    CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG
    ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA
    GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC
    TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG
    GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG
    AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG
    CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT
    TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT
    CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT
    CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC
    CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG
    GGCTCCTTCA GCACCAGCTC GTTGTCTAGT TTGGGAGCCT CTGGTGGAGC AAGTAACTGG
    GATTCGTACA GTGACCATTT CACCATTGAA ACCTGCAAAG AGACGGACAT GCTGAACTAC
    CTCATCGAGT GTTTTGACCG AGTTGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC
    CAGCCAGCAG TCAGCCAGCT TCTGAGCAAC ATCCGCTCGC AGTGCATCTC CCACACCGCA
    CTAGTGCTAC AAGGTTCCCT CACACAGCCC AGGTCCTTGC AACAGCCATC CTTCCTCGTG
    CCGTACATGC TCTGCAGGAA CCTCCCGTAC GGCTTCATTC AAGAGCTGGT GAGGACCACT
    CACCAGGATG AAGAGGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCGCTG
    GCTGCCAAAG AGTGCTCCTT GGAAAGCGAC TACTTTAAGT ACCCCCTCAT GGCACTGGGT
    GAACTCTGTG AAACCAAGTT TGGGAAGACA CACCCTATGT GCAATTTGGT CGCTTCTTTA
    CCCCTGTGGT TGCCGAAGTC CTTAAGCCCT GGTTCTGGGC GGGAGCTGCA GAGACTCTCC
    TACTTAGGGG CCTTCTTTAG CTTCTCAGTC TTCGCAGAGG ACGATGCAAA GGTGGTTGAG
    AAATACTTCT CAGGGCCTGC CATTACCCTG GAGAACACAC GCGTGGTCAG CCAGTCACTA
    CAGCATTACC TGGAGCTGGG ACGGCAAGAA CTTTTCAAGA TTCTACATAG TATTTTGTTA
    AATGGCGAAA CTCGTGAAGC GGCTCTCAGC TACATGGCGG CCCTTGTCAA TGCCAACATG
    AAGAAAGCCC AGATGCAGGC AGATGATCGG TTGGTGTCTA CAGACGGGTT TATGCTGAAT
    CTCCTTTGGG TCCTGCAGCA GCTAAGTACA AAAATCAAGT TAGAAACAGT TGACCCCACT
    TACATATTCC ACCCAAGATG TCGGATCACT CTCCCGAATG ATGAGACACG GATAAATGCA
    ACGATGGAGG ATGTGAATGA GCGGCTGACA GAGCTCTATG GTGATCAGCC TCCATTTTCT
    GAGCCAAAAT TCCCTACGGA ATGTTTCTTT CTCACCCTGC ATGCTCATCA CCTCTCTATT
    CTGCCTAGTT GTCGTCGCTA CATTCGCCGA CTTCGAGCCA TCCGCGAGCT CAATAGAACC
    GTGGAAGATT TGAAAAATAA TGAAAGCCAG TGGAAAGATT CCCCACTGGC CACCAGACAC
    CGTGAAATGC TGAAGCGCTG TAAAACTCAG CTTAAGAAAC TGGTACGGTG CAAGGCCTGT
    GCTGACGCTG GCTTACTTGA CGAGAGCTTC CTAAGAAGAT GCCTGAATTT TTATGGCCTT
    CTCATTCAGC TGATGCTGCG CATCCTGGAC CCTGCATATC CTGACGTAAC ACTGCCTTTG
    AATTCAGAAG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTCGA AGATGTTGCT
    GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TTTATGAGCC CTGCACTCAG
    GACATTGTCA TGTTCCTCGT TGTGATGCTG TGCAACCAGA ACTACATCCG AAACCCTTAC
    CTAGTGGCCA AGCTGGTGGA GGTCATGTTC ATGACCAACC CTTCTGTCCA GCCGAGAACT
    CAGAAGTTCT TTGAGATGAT TGAGAACCAT CCTCTCTCTA CCAAACTGCT GGTGCCGTCC
    CTCATGAAGT TCTATACAGA TGTCGAGCAT ACTGGAGCCA CCAGCGAGTT CTATGACAAG
    TTCACAATTC GCTATCACAT TAGCACTATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC
    CATGGCACCT TCATGGAGGA GTTCAATTCT GGAAAGCAGT TCGTTCGGTA TATCAATATG
    CTAATAAATG ACACGACCTT TTTACTGGAC GAAAGTCTGG AGTCTCTGAA GCGGATCCAT
    GAAGTGCAAG AAGAGATGAA GAACAAAGAA CAGTGGGACC AGCTGCCTCG GGATCAGCAG
    CAGGCCCGGC AGTCTCAGCT TGCTCAGGAC GAGCGTGTTT CCCGTTCTTA TCTTGCCCTG
    GCAACTGAAA CCGTGGACAT GTTCCATCTC CTCACCAAGC AAGTCCAGAA GCCGTTCCTG
    AGGCCAGAGC TTGGTCCCCG ATTAGCAGCC ATGCTGAACT TTAACCTGCA GCAGCTCTGC
    GGACCCAAGT GCCGGGACCT GAAAGTTGAA AACCCAGAGA AGTATGGTTT TGAGCCAAAG
    AAGCTGTTGG ACCAACTGAC AGATATTTAT TTACAATTGG ACTGTGCTCG CTTTGCTAAA
    GCCATCGCCG ATGACCAGCG ATCCTACAGC AAGGAACTGT TTGAAGAAGT CATTTCAAAG
    ATGCGGAAGG CGGGAATCAA ATCCACCATC GCAATAGAGA AGTTTAAACT TCTTGCAGAG
    AAAGTGGAGG AAATCGTGGC AAAGAATGCG CGGGCAGAAA TAGACTACAG CGATGCCCCG
    GACGAGTTCA GAGACCCTCT GATGGACACC CTGATGACCG ATCCCGTGAG ACTGCCCTCT
    GGCACCGTCA TGGACCGTTC TATCATCCTG CGGCATCTGC TCAACTCCCC CACCGACCCC
    TTCAACCGCC AGATGCTGAC TGAGAGCATG CTGGAGCCAG TGCCAGAGCT AAAGGAGCAG
    ATTCAGGCCT GGATGAGAGA GAAACAGAGC AGTGACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu34371
    Clone ID Related Accession (Same CDS sequence) XM_006539088.4 , XM_006539088.3
    Accession Version XM_006539088.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ubiquitin conjugation factor E4 B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539088.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
    CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT
    CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG
    ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA
    GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC
    TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG
    GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG
    AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG
    CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT
    TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT
    CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT
    CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC
    CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG
    GGCTCCTTCA GCACCAGCTC GTTGTCTAGC CTCTATGAAA CGAGCCCCGC CCCCTCTGCC
    AGTTTATGGA GTTTTGTGCC AGCGCTCAGC CCATCCTTTG CCTTACCTTC CCACCCAGCC
    CCACAGGCGG CTATGCCTTC CCAGTCCCTT AGCCCCCATG GTGTAGCCCC ACAGGCGGCT
    GTGCCTTCCC AGTCCCTCAG CCCCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCAG
    TCCCTCAGCC CCCATGGTGT AGCCCCACAG GTGGCTGTGC CTTCCCAGTC CCTCAGCACC
    CATGGTGTAG CCCCGCAGGC AGCTGTGCCT TCCCAGTCCC TCAGCACCCA TGGTGTAGCC
    CCGCAGGCGG CTGTGCCTTC CCAGTCCCTC AGCACCCATG GTGTAGCCCC GCAGGTGGCT
    GTGCCTTCCC AGTCCCTCAG CACCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCCA
    CCCCTCAGCC CTCATAGTAC AGCTTCTGGA AATGCTGCCG GAAGCCAGCC CTCATCCCCG
    AGGTATCGGC CCTATACTGT CACGCACCCT TGGGGGTCTT CACCTTGCCC CACCCCACGA
    TCCTCCATTC TGGCTAGCTC CCCAGGCTTC CCTTCCCACG CAAGTAGTCC CCGGGCTGTA
    CCTGCCAGCT CCTCCAGACA GAGGCCCCGC AGCAGGGTCC CCGCCTTCCC ACCTGCCTCG
    CCCAGTGCCG CCAGCAGACG TCCCTCCTCC CTGCGGATCT CTCCTAGTTT GGGAGCCTCT
    GGTGGAGCAA GTAACTGGGA TTCGTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG
    ACGGACATGC TGAACTACCT CATCGAGTGT TTTGACCGAG TTGGAATAGA GGAAAAAAAA
    GCACCAAAGA TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCGCAG
    TGCATCTCCC ACACCGCACT AGTGCTACAA GGTTCCCTCA CACAGCCCAG GTCCTTGCAA
    CAGCCATCCT TCCTCGTGCC GTACATGCTC TGCAGGAACC TCCCGTACGG CTTCATTCAA
    GAGCTGGTGA GGACCACTCA CCAGGATGAA GAGGTGTTCA AGCAGATCTT TATCCCCATT
    TTACAAGGCC TGGCGCTGGC TGCCAAAGAG TGCTCCTTGG AAAGCGACTA CTTTAAGTAC
    CCCCTCATGG CACTGGGTGA ACTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTATGTGC
    AATTTGGTCG CTTCTTTACC CCTGTGGTTG CCGAAGTCCT TAAGCCCTGG TTCTGGGCGG
    GAGCTGCAGA GACTCTCCTA CTTAGGGGCC TTCTTTAGCT TCTCAGTCTT CGCAGAGGAC
    GATGCAAAGG TGGTTGAGAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA GAACACACGC
    GTGGTCAGCC AGTCACTACA GCATTACCTG GAGCTGGGAC GGCAAGAACT TTTCAAGATT
    CTACATAGTA TTTTGTTAAA TGGCGAAACT CGTGAAGCGG CTCTCAGCTA CATGGCGGCC
    CTTGTCAATG CCAACATGAA GAAAGCCCAG ATGCAGGCAG ATGATCGGTT GGTGTCTACA
    GACGGGTTTA TGCTGAATCT CCTTTGGGTC CTGCAGCAGC TAAGTACAAA AATCAAGTTA
    GAAACAGTTG ACCCCACTTA CATATTCCAC CCAAGATGTC GGATCACTCT CCCGAATGAT
    GAGACACGGA TAAATGCAAC GATGGAGGAT GTGAATGAGC GGCTGACAGA GCTCTATGGT
    GATCAGCCTC CATTTTCTGA GCCAAAATTC CCTACGGAAT GTTTCTTTCT CACCCTGCAT
    GCTCATCACC TCTCTATTCT GCCTAGTTGT CGTCGCTACA TTCGCCGACT TCGAGCCATC
    CGCGAGCTCA ATAGAACCGT GGAAGATTTG AAAAATAATG AAAGCCAGTG GAAAGATTCC
    CCACTGGCCA CCAGACACCG TGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG
    GTACGGTGCA AGGCCTGTGC TGACGCTGGC TTACTTGACG AGAGCTTCCT AAGAAGATGC
    CTGAATTTTT ATGGCCTTCT CATTCAGCTG ATGCTGCGCA TCCTGGACCC TGCATATCCT
    GACGTAACAC TGCCTTTGAA TTCAGAAGTC CCCAAGGTAT TTGCAGCATT GCCTGAGTTT
    TATGTCGAAG ATGTTGCTGA ATTTTTATTT TTTATTGTAC AATACTCTCC TCAGGTGCTT
    TATGAGCCCT GCACTCAGGA CATTGTCATG TTCCTCGTTG TGATGCTGTG CAACCAGAAC
    TACATCCGAA ACCCTTACCT AGTGGCCAAG CTGGTGGAGG TCATGTTCAT GACCAACCCT
    TCTGTCCAGC CGAGAACTCA GAAGTTCTTT GAGATGATTG AGAACCATCC TCTCTCTACC
    AAACTGCTGG TGCCGTCCCT CATGAAGTTC TATACAGATG TCGAGCATAC TGGAGCCACC
    AGCGAGTTCT ATGACAAGTT CACAATTCGC TATCACATTA GCACTATTTT TAAAAGCCTT
    TGGCAAAACA TAGCTCACCA TGGCACCTTC ATGGAGGAGT TCAATTCTGG AAAGCAGTTC
    GTTCGGTATA TCAATATGCT AATAAATGAC ACGACCTTTT TACTGGACGA AAGTCTGGAG
    TCTCTGAAGC GGATCCATGA AGTGCAAGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG
    CTGCCTCGGG ATCAGCAGCA GGCCCGGCAG TCTCAGCTTG CTCAGGACGA GCGTGTTTCC
    CGTTCTTATC TTGCCCTGGC AACTGAAACC GTGGACATGT TCCATCTCCT CACCAAGCAA
    GTCCAGAAGC CGTTCCTGAG GCCAGAGCTT GGTCCCCGAT TAGCAGCCAT GCTGAACTTT
    AACCTGCAGC AGCTCTGCGG ACCCAAGTGC CGGGACCTGA AAGTTGAAAA CCCAGAGAAG
    TATGGTTTTG AGCCAAAGAA GCTGTTGGAC CAACTGACAG ATATTTATTT ACAATTGGAC
    TGTGCTCGCT TTGCTAAAGC CATCGCCGAT GACCAGCGAT CCTACAGCAA GGAACTGTTT
    GAAGAAGTCA TTTCAAAGAT GCGGAAGGCG GGAATCAAAT CCACCATCGC AATAGAGAAG
    TTTAAACTTC TTGCAGAGAA AGTGGAGGAA ATCGTGGCAA AGAATGCGCG GGCAGAAATA
    GACTACAGCG ATGCCCCGGA CGAGTTCAGA GACCCTCTGA TGGACACCCT GATGACCGAT
    CCCGTGAGAC TGCCCTCTGG CACCGTCATG GACCGTTCTA TCATCCTGCG GCATCTGCTC
    AACTCCCCCA CCGACCCCTT CAACCGCCAG ATGCTGACTG AGAGCATGCT GGAGCCAGTG
    CCAGAGCTAA AGGAGCAGAT TCAGGCCTGG ATGAGAGAGA AACAGAGCAG TGACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006539151.1
    CDS2257..6516
    Translation

    Target ORF information:

    RefSeq Version XM_006539088.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006539088.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
    CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT
    CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG
    ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA
    GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC
    TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG
    GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG
    AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG
    CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT
    TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT
    CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT
    CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC
    CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG
    GGCTCCTTCA GCACCAGCTC GTTGTCTAGC CTCTATGAAA CGAGCCCCGC CCCCTCTGCC
    AGTTTATGGA GTTTTGTGCC AGCGCTCAGC CCATCCTTTG CCTTACCTTC CCACCCAGCC
    CCACAGGCGG CTATGCCTTC CCAGTCCCTT AGCCCCCATG GTGTAGCCCC ACAGGCGGCT
    GTGCCTTCCC AGTCCCTCAG CCCCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCAG
    TCCCTCAGCC CCCATGGTGT AGCCCCACAG GTGGCTGTGC CTTCCCAGTC CCTCAGCACC
    CATGGTGTAG CCCCGCAGGC AGCTGTGCCT TCCCAGTCCC TCAGCACCCA TGGTGTAGCC
    CCGCAGGCGG CTGTGCCTTC CCAGTCCCTC AGCACCCATG GTGTAGCCCC GCAGGTGGCT
    GTGCCTTCCC AGTCCCTCAG CACCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCCA
    CCCCTCAGCC CTCATAGTAC AGCTTCTGGA AATGCTGCCG GAAGCCAGCC CTCATCCCCG
    AGGTATCGGC CCTATACTGT CACGCACCCT TGGGGGTCTT CACCTTGCCC CACCCCACGA
    TCCTCCATTC TGGCTAGCTC CCCAGGCTTC CCTTCCCACG CAAGTAGTCC CCGGGCTGTA
    CCTGCCAGCT CCTCCAGACA GAGGCCCCGC AGCAGGGTCC CCGCCTTCCC ACCTGCCTCG
    CCCAGTGCCG CCAGCAGACG TCCCTCCTCC CTGCGGATCT CTCCTAGTTT GGGAGCCTCT
    GGTGGAGCAA GTAACTGGGA TTCGTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG
    ACGGACATGC TGAACTACCT CATCGAGTGT TTTGACCGAG TTGGAATAGA GGAAAAAAAA
    GCACCAAAGA TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCGCAG
    TGCATCTCCC ACACCGCACT AGTGCTACAA GGTTCCCTCA CACAGCCCAG GTCCTTGCAA
    CAGCCATCCT TCCTCGTGCC GTACATGCTC TGCAGGAACC TCCCGTACGG CTTCATTCAA
    GAGCTGGTGA GGACCACTCA CCAGGATGAA GAGGTGTTCA AGCAGATCTT TATCCCCATT
    TTACAAGGCC TGGCGCTGGC TGCCAAAGAG TGCTCCTTGG AAAGCGACTA CTTTAAGTAC
    CCCCTCATGG CACTGGGTGA ACTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTATGTGC
    AATTTGGTCG CTTCTTTACC CCTGTGGTTG CCGAAGTCCT TAAGCCCTGG TTCTGGGCGG
    GAGCTGCAGA GACTCTCCTA CTTAGGGGCC TTCTTTAGCT TCTCAGTCTT CGCAGAGGAC
    GATGCAAAGG TGGTTGAGAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA GAACACACGC
    GTGGTCAGCC AGTCACTACA GCATTACCTG GAGCTGGGAC GGCAAGAACT TTTCAAGATT
    CTACATAGTA TTTTGTTAAA TGGCGAAACT CGTGAAGCGG CTCTCAGCTA CATGGCGGCC
    CTTGTCAATG CCAACATGAA GAAAGCCCAG ATGCAGGCAG ATGATCGGTT GGTGTCTACA
    GACGGGTTTA TGCTGAATCT CCTTTGGGTC CTGCAGCAGC TAAGTACAAA AATCAAGTTA
    GAAACAGTTG ACCCCACTTA CATATTCCAC CCAAGATGTC GGATCACTCT CCCGAATGAT
    GAGACACGGA TAAATGCAAC GATGGAGGAT GTGAATGAGC GGCTGACAGA GCTCTATGGT
    GATCAGCCTC CATTTTCTGA GCCAAAATTC CCTACGGAAT GTTTCTTTCT CACCCTGCAT
    GCTCATCACC TCTCTATTCT GCCTAGTTGT CGTCGCTACA TTCGCCGACT TCGAGCCATC
    CGCGAGCTCA ATAGAACCGT GGAAGATTTG AAAAATAATG AAAGCCAGTG GAAAGATTCC
    CCACTGGCCA CCAGACACCG TGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG
    GTACGGTGCA AGGCCTGTGC TGACGCTGGC TTACTTGACG AGAGCTTCCT AAGAAGATGC
    CTGAATTTTT ATGGCCTTCT CATTCAGCTG ATGCTGCGCA TCCTGGACCC TGCATATCCT
    GACGTAACAC TGCCTTTGAA TTCAGAAGTC CCCAAGGTAT TTGCAGCATT GCCTGAGTTT
    TATGTCGAAG ATGTTGCTGA ATTTTTATTT TTTATTGTAC AATACTCTCC TCAGGTGCTT
    TATGAGCCCT GCACTCAGGA CATTGTCATG TTCCTCGTTG TGATGCTGTG CAACCAGAAC
    TACATCCGAA ACCCTTACCT AGTGGCCAAG CTGGTGGAGG TCATGTTCAT GACCAACCCT
    TCTGTCCAGC CGAGAACTCA GAAGTTCTTT GAGATGATTG AGAACCATCC TCTCTCTACC
    AAACTGCTGG TGCCGTCCCT CATGAAGTTC TATACAGATG TCGAGCATAC TGGAGCCACC
    AGCGAGTTCT ATGACAAGTT CACAATTCGC TATCACATTA GCACTATTTT TAAAAGCCTT
    TGGCAAAACA TAGCTCACCA TGGCACCTTC ATGGAGGAGT TCAATTCTGG AAAGCAGTTC
    GTTCGGTATA TCAATATGCT AATAAATGAC ACGACCTTTT TACTGGACGA AAGTCTGGAG
    TCTCTGAAGC GGATCCATGA AGTGCAAGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG
    CTGCCTCGGG ATCAGCAGCA GGCCCGGCAG TCTCAGCTTG CTCAGGACGA GCGTGTTTCC
    CGTTCTTATC TTGCCCTGGC AACTGAAACC GTGGACATGT TCCATCTCCT CACCAAGCAA
    GTCCAGAAGC CGTTCCTGAG GCCAGAGCTT GGTCCCCGAT TAGCAGCCAT GCTGAACTTT
    AACCTGCAGC AGCTCTGCGG ACCCAAGTGC CGGGACCTGA AAGTTGAAAA CCCAGAGAAG
    TATGGTTTTG AGCCAAAGAA GCTGTTGGAC CAACTGACAG ATATTTATTT ACAATTGGAC
    TGTGCTCGCT TTGCTAAAGC CATCGCCGAT GACCAGCGAT CCTACAGCAA GGAACTGTTT
    GAAGAAGTCA TTTCAAAGAT GCGGAAGGCG GGAATCAAAT CCACCATCGC AATAGAGAAG
    TTTAAACTTC TTGCAGAGAA AGTGGAGGAA ATCGTGGCAA AGAATGCGCG GGCAGAAATA
    GACTACAGCG ATGCCCCGGA CGAGTTCAGA GACCCTCTGA TGGACACCCT GATGACCGAT
    CCCGTGAGAC TGCCCTCTGG CACCGTCATG GACCGTTCTA TCATCCTGCG GCATCTGCTC
    AACTCCCCCA CCGACCCCTT CAACCGCCAG ATGCTGACTG AGAGCATGCT GGAGCCAGTG
    CCAGAGCTAA AGGAGCAGAT TCAGGCCTGG ATGAGAGAGA AACAGAGCAG TGACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu34371
    Clone ID Related Accession (Same CDS sequence) XM_006539088.4 , XM_006539088.3
    Accession Version XM_006539088.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product ubiquitin conjugation factor E4 B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
    CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT
    CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG
    ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA
    GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC
    TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG
    GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG
    AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG
    CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT
    TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT
    CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT
    CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC
    CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG
    GGCTCCTTCA GCACCAGCTC GTTGTCTAGC CTCTATGAAA CGAGCCCCGC CCCCTCTGCC
    AGTTTATGGA GTTTTGTGCC AGCGCTCAGC CCATCCTTTG CCTTACCTTC CCACCCAGCC
    CCACAGGCGG CTATGCCTTC CCAGTCCCTT AGCCCCCATG GTGTAGCCCC ACAGGCGGCT
    GTGCCTTCCC AGTCCCTCAG CCCCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCAG
    TCCCTCAGCC CCCATGGTGT AGCCCCACAG GTGGCTGTGC CTTCCCAGTC CCTCAGCACC
    CATGGTGTAG CCCCGCAGGC AGCTGTGCCT TCCCAGTCCC TCAGCACCCA TGGTGTAGCC
    CCGCAGGCGG CTGTGCCTTC CCAGTCCCTC AGCACCCATG GTGTAGCCCC GCAGGTGGCT
    GTGCCTTCCC AGTCCCTCAG CACCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCCA
    CCCCTCAGCC CTCATAGTAC AGCTTCTGGA AATGCTGCCG GAAGCCAGCC CTCATCCCCG
    AGGTATCGGC CCTATACTGT CACGCACCCT TGGGGGTCTT CACCTTGCCC CACCCCACGA
    TCCTCCATTC TGGCTAGCTC CCCAGGCTTC CCTTCCCACG CAAGTAGTCC CCGGGCTGTA
    CCTGCCAGCT CCTCCAGACA GAGGCCCCGC AGCAGGGTCC CCGCCTTCCC ACCTGCCTCG
    CCCAGTGCCG CCAGCAGACG TCCCTCCTCC CTGCGGATCT CTCCTAGTTT GGGAGCCTCT
    GGTGGAGCAA GTAACTGGGA TTCGTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG
    ACGGACATGC TGAACTACCT CATCGAGTGT TTTGACCGAG TTGGAATAGA GGAAAAAAAA
    GCACCAAAGA TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCGCAG
    TGCATCTCCC ACACCGCACT AGTGCTACAA GGTTCCCTCA CACAGCCCAG GTCCTTGCAA
    CAGCCATCCT TCCTCGTGCC GTACATGCTC TGCAGGAACC TCCCGTACGG CTTCATTCAA
    GAGCTGGTGA GGACCACTCA CCAGGATGAA GAGGTGTTCA AGCAGATCTT TATCCCCATT
    TTACAAGGCC TGGCGCTGGC TGCCAAAGAG TGCTCCTTGG AAAGCGACTA CTTTAAGTAC
    CCCCTCATGG CACTGGGTGA ACTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTATGTGC
    AATTTGGTCG CTTCTTTACC CCTGTGGTTG CCGAAGTCCT TAAGCCCTGG TTCTGGGCGG
    GAGCTGCAGA GACTCTCCTA CTTAGGGGCC TTCTTTAGCT TCTCAGTCTT CGCAGAGGAC
    GATGCAAAGG TGGTTGAGAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA GAACACACGC
    GTGGTCAGCC AGTCACTACA GCATTACCTG GAGCTGGGAC GGCAAGAACT TTTCAAGATT
    CTACATAGTA TTTTGTTAAA TGGCGAAACT CGTGAAGCGG CTCTCAGCTA CATGGCGGCC
    CTTGTCAATG CCAACATGAA GAAAGCCCAG ATGCAGGCAG ATGATCGGTT GGTGTCTACA
    GACGGGTTTA TGCTGAATCT CCTTTGGGTC CTGCAGCAGC TAAGTACAAA AATCAAGTTA
    GAAACAGTTG ACCCCACTTA CATATTCCAC CCAAGATGTC GGATCACTCT CCCGAATGAT
    GAGACACGGA TAAATGCAAC GATGGAGGAT GTGAATGAGC GGCTGACAGA GCTCTATGGT
    GATCAGCCTC CATTTTCTGA GCCAAAATTC CCTACGGAAT GTTTCTTTCT CACCCTGCAT
    GCTCATCACC TCTCTATTCT GCCTAGTTGT CGTCGCTACA TTCGCCGACT TCGAGCCATC
    CGCGAGCTCA ATAGAACCGT GGAAGATTTG AAAAATAATG AAAGCCAGTG GAAAGATTCC
    CCACTGGCCA CCAGACACCG TGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG
    GTACGGTGCA AGGCCTGTGC TGACGCTGGC TTACTTGACG AGAGCTTCCT AAGAAGATGC
    CTGAATTTTT ATGGCCTTCT CATTCAGCTG ATGCTGCGCA TCCTGGACCC TGCATATCCT
    GACGTAACAC TGCCTTTGAA TTCAGAAGTC CCCAAGGTAT TTGCAGCATT GCCTGAGTTT
    TATGTCGAAG ATGTTGCTGA ATTTTTATTT TTTATTGTAC AATACTCTCC TCAGGTGCTT
    TATGAGCCCT GCACTCAGGA CATTGTCATG TTCCTCGTTG TGATGCTGTG CAACCAGAAC
    TACATCCGAA ACCCTTACCT AGTGGCCAAG CTGGTGGAGG TCATGTTCAT GACCAACCCT
    TCTGTCCAGC CGAGAACTCA GAAGTTCTTT GAGATGATTG AGAACCATCC TCTCTCTACC
    AAACTGCTGG TGCCGTCCCT CATGAAGTTC TATACAGATG TCGAGCATAC TGGAGCCACC
    AGCGAGTTCT ATGACAAGTT CACAATTCGC TATCACATTA GCACTATTTT TAAAAGCCTT
    TGGCAAAACA TAGCTCACCA TGGCACCTTC ATGGAGGAGT TCAATTCTGG AAAGCAGTTC
    GTTCGGTATA TCAATATGCT AATAAATGAC ACGACCTTTT TACTGGACGA AAGTCTGGAG
    TCTCTGAAGC GGATCCATGA AGTGCAAGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG
    CTGCCTCGGG ATCAGCAGCA GGCCCGGCAG TCTCAGCTTG CTCAGGACGA GCGTGTTTCC
    CGTTCTTATC TTGCCCTGGC AACTGAAACC GTGGACATGT TCCATCTCCT CACCAAGCAA
    GTCCAGAAGC CGTTCCTGAG GCCAGAGCTT GGTCCCCGAT TAGCAGCCAT GCTGAACTTT
    AACCTGCAGC AGCTCTGCGG ACCCAAGTGC CGGGACCTGA AAGTTGAAAA CCCAGAGAAG
    TATGGTTTTG AGCCAAAGAA GCTGTTGGAC CAACTGACAG ATATTTATTT ACAATTGGAC
    TGTGCTCGCT TTGCTAAAGC CATCGCCGAT GACCAGCGAT CCTACAGCAA GGAACTGTTT
    GAAGAAGTCA TTTCAAAGAT GCGGAAGGCG GGAATCAAAT CCACCATCGC AATAGAGAAG
    TTTAAACTTC TTGCAGAGAA AGTGGAGGAA ATCGTGGCAA AGAATGCGCG GGCAGAAATA
    GACTACAGCG ATGCCCCGGA CGAGTTCAGA GACCCTCTGA TGGACACCCT GATGACCGAT
    CCCGTGAGAC TGCCCTCTGG CACCGTCATG GACCGTTCTA TCATCCTGCG GCATCTGCTC
    AACTCCCCCA CCGACCCCTT CAACCGCCAG ATGCTGACTG AGAGCATGCT GGAGCCAGTG
    CCAGAGCTAA AGGAGCAGAT TCAGGCCTGG ATGAGAGAGA AACAGAGCAG TGACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006539151.1
    CDS725..4984
    Translation

    Target ORF information:

    RefSeq Version XM_006539088.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006539088.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA 
    CAGACCTCCC AGCCGACCAC CCCGCTTACA TCTCCCCAGA GGGAGAACCC TCCGGGACCT
    CCAATAGCTG CATCAGCCCC AGGCCCCTCC CAGAGTCTTG GTCTCAATGT CCACAACATG
    ACCCCAGCTA CCTCCCCCAT AGGTGCAGCA GGAGTTGCCC ATCGGAGCCA GAGCAGCGAA
    GGCGTCAGTT CTCTCAGCAG CTCACCTTCC AACAGCCTTG AGACACAGTC TCAGTCCCTC
    TCCCGTTCCC AGAGCATGGA TATTGACGGT GTCTCCTGTG AGAAAAGCAT GTCCCAGGTG
    GATGTGGATT CGGGAATTGA GAATATGGAG GTGGACGAGA ACGACCGAAG AGAAAAACGG
    AGCCTGAGTG ACAAGGAGCC TTCTTCAGGT CCTGAGGTGT CTGAAGAGCA AGCCTTACAG
    CTGGTCTGTA AGATCTTCCG TGTCTCCTGG AAGGACCGCG ACAGAGATGT CATCTTCCTT
    TCTTCTCTCT CTGCGCAGTT TAAACAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAT
    CTGATTGGCC AGATTTTAAT GGAAGTACTG ATGATGTCAA CTCAGACACG AGATGAAAAT
    CCATTTGCCA GTCTGACAGC CACATCCCAG CCCATCGCCA CGGCAGCTCG GTCACCAGAC
    CGCAATCTCA TGCTGAACAC TGGCTCCAGT TCAGGAACAA GCCCCATGTT CTGCAACATG
    GGCTCCTTCA GCACCAGCTC GTTGTCTAGC CTCTATGAAA CGAGCCCCGC CCCCTCTGCC
    AGTTTATGGA GTTTTGTGCC AGCGCTCAGC CCATCCTTTG CCTTACCTTC CCACCCAGCC
    CCACAGGCGG CTATGCCTTC CCAGTCCCTT AGCCCCCATG GTGTAGCCCC ACAGGCGGCT
    GTGCCTTCCC AGTCCCTCAG CCCCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCAG
    TCCCTCAGCC CCCATGGTGT AGCCCCACAG GTGGCTGTGC CTTCCCAGTC CCTCAGCACC
    CATGGTGTAG CCCCGCAGGC AGCTGTGCCT TCCCAGTCCC TCAGCACCCA TGGTGTAGCC
    CCGCAGGCGG CTGTGCCTTC CCAGTCCCTC AGCACCCATG GTGTAGCCCC GCAGGTGGCT
    GTGCCTTCCC AGTCCCTCAG CACCCATGGT GTAGCCCCGC AGGCGGCTGT GCCTTCCCCA
    CCCCTCAGCC CTCATAGTAC AGCTTCTGGA AATGCTGCCG GAAGCCAGCC CTCATCCCCG
    AGGTATCGGC CCTATACTGT CACGCACCCT TGGGGGTCTT CACCTTGCCC CACCCCACGA
    TCCTCCATTC TGGCTAGCTC CCCAGGCTTC CCTTCCCACG CAAGTAGTCC CCGGGCTGTA
    CCTGCCAGCT CCTCCAGACA GAGGCCCCGC AGCAGGGTCC CCGCCTTCCC ACCTGCCTCG
    CCCAGTGCCG CCAGCAGACG TCCCTCCTCC CTGCGGATCT CTCCTAGTTT GGGAGCCTCT
    GGTGGAGCAA GTAACTGGGA TTCGTACAGT GACCATTTCA CCATTGAAAC CTGCAAAGAG
    ACGGACATGC TGAACTACCT CATCGAGTGT TTTGACCGAG TTGGAATAGA GGAAAAAAAA
    GCACCAAAGA TGTGCAGCCA GCCAGCAGTC AGCCAGCTTC TGAGCAACAT CCGCTCGCAG
    TGCATCTCCC ACACCGCACT AGTGCTACAA GGTTCCCTCA CACAGCCCAG GTCCTTGCAA
    CAGCCATCCT TCCTCGTGCC GTACATGCTC TGCAGGAACC TCCCGTACGG CTTCATTCAA
    GAGCTGGTGA GGACCACTCA CCAGGATGAA GAGGTGTTCA AGCAGATCTT TATCCCCATT
    TTACAAGGCC TGGCGCTGGC TGCCAAAGAG TGCTCCTTGG AAAGCGACTA CTTTAAGTAC
    CCCCTCATGG CACTGGGTGA ACTCTGTGAA ACCAAGTTTG GGAAGACACA CCCTATGTGC
    AATTTGGTCG CTTCTTTACC CCTGTGGTTG CCGAAGTCCT TAAGCCCTGG TTCTGGGCGG
    GAGCTGCAGA GACTCTCCTA CTTAGGGGCC TTCTTTAGCT TCTCAGTCTT CGCAGAGGAC
    GATGCAAAGG TGGTTGAGAA ATACTTCTCA GGGCCTGCCA TTACCCTGGA GAACACACGC
    GTGGTCAGCC AGTCACTACA GCATTACCTG GAGCTGGGAC GGCAAGAACT TTTCAAGATT
    CTACATAGTA TTTTGTTAAA TGGCGAAACT CGTGAAGCGG CTCTCAGCTA CATGGCGGCC
    CTTGTCAATG CCAACATGAA GAAAGCCCAG ATGCAGGCAG ATGATCGGTT GGTGTCTACA
    GACGGGTTTA TGCTGAATCT CCTTTGGGTC CTGCAGCAGC TAAGTACAAA AATCAAGTTA
    GAAACAGTTG ACCCCACTTA CATATTCCAC CCAAGATGTC GGATCACTCT CCCGAATGAT
    GAGACACGGA TAAATGCAAC GATGGAGGAT GTGAATGAGC GGCTGACAGA GCTCTATGGT
    GATCAGCCTC CATTTTCTGA GCCAAAATTC CCTACGGAAT GTTTCTTTCT CACCCTGCAT
    GCTCATCACC TCTCTATTCT GCCTAGTTGT CGTCGCTACA TTCGCCGACT TCGAGCCATC
    CGCGAGCTCA ATAGAACCGT GGAAGATTTG AAAAATAATG AAAGCCAGTG GAAAGATTCC
    CCACTGGCCA CCAGACACCG TGAAATGCTG AAGCGCTGTA AAACTCAGCT TAAGAAACTG
    GTACGGTGCA AGGCCTGTGC TGACGCTGGC TTACTTGACG AGAGCTTCCT AAGAAGATGC
    CTGAATTTTT ATGGCCTTCT CATTCAGCTG ATGCTGCGCA TCCTGGACCC TGCATATCCT
    GACGTAACAC TGCCTTTGAA TTCAGAAGTC CCCAAGGTAT TTGCAGCATT GCCTGAGTTT
    TATGTCGAAG ATGTTGCTGA ATTTTTATTT TTTATTGTAC AATACTCTCC TCAGGTGCTT
    TATGAGCCCT GCACTCAGGA CATTGTCATG TTCCTCGTTG TGATGCTGTG CAACCAGAAC
    TACATCCGAA ACCCTTACCT AGTGGCCAAG CTGGTGGAGG TCATGTTCAT GACCAACCCT
    TCTGTCCAGC CGAGAACTCA GAAGTTCTTT GAGATGATTG AGAACCATCC TCTCTCTACC
    AAACTGCTGG TGCCGTCCCT CATGAAGTTC TATACAGATG TCGAGCATAC TGGAGCCACC
    AGCGAGTTCT ATGACAAGTT CACAATTCGC TATCACATTA GCACTATTTT TAAAAGCCTT
    TGGCAAAACA TAGCTCACCA TGGCACCTTC ATGGAGGAGT TCAATTCTGG AAAGCAGTTC
    GTTCGGTATA TCAATATGCT AATAAATGAC ACGACCTTTT TACTGGACGA AAGTCTGGAG
    TCTCTGAAGC GGATCCATGA AGTGCAAGAA GAGATGAAGA ACAAAGAACA GTGGGACCAG
    CTGCCTCGGG ATCAGCAGCA GGCCCGGCAG TCTCAGCTTG CTCAGGACGA GCGTGTTTCC
    CGTTCTTATC TTGCCCTGGC AACTGAAACC GTGGACATGT TCCATCTCCT CACCAAGCAA
    GTCCAGAAGC CGTTCCTGAG GCCAGAGCTT GGTCCCCGAT TAGCAGCCAT GCTGAACTTT
    AACCTGCAGC AGCTCTGCGG ACCCAAGTGC CGGGACCTGA AAGTTGAAAA CCCAGAGAAG
    TATGGTTTTG AGCCAAAGAA GCTGTTGGAC CAACTGACAG ATATTTATTT ACAATTGGAC
    TGTGCTCGCT TTGCTAAAGC CATCGCCGAT GACCAGCGAT CCTACAGCAA GGAACTGTTT
    GAAGAAGTCA TTTCAAAGAT GCGGAAGGCG GGAATCAAAT CCACCATCGC AATAGAGAAG
    TTTAAACTTC TTGCAGAGAA AGTGGAGGAA ATCGTGGCAA AGAATGCGCG GGCAGAAATA
    GACTACAGCG ATGCCCCGGA CGAGTTCAGA GACCCTCTGA TGGACACCCT GATGACCGAT
    CCCGTGAGAC TGCCCTCTGG CACCGTCATG GACCGTTCTA TCATCCTGCG GCATCTGCTC
    AACTCCCCCA CCGACCCCTT CAACCGCCAG ATGCTGACTG AGAGCATGCT GGAGCCAGTG
    CCAGAGCTAA AGGAGCAGAT TCAGGCCTGG ATGAGAGAGA AACAGAGCAG TGACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu34372
    Clone ID Related Accession (Same CDS sequence) XM_006539089.3
    Accession Version XM_006539089.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product ubiquitin conjugation factor E4 B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATTCGAC GGAGGCGCCT GGCACGACTT GCTGGTGGAC AGACCTCCCA GCCGACCACC 
    CCGCTTACAT CTCCCCAGAG GGAGAACCCT CCGGGACCTC CAATAGCTGC ATCAGCCCCA
    GGCCCCTCCC AGAGTCTTGG TCTCAATGTC CACAACATGA CCCCAGCTAC CTCCCCCATA
    GGTGCAGCAG GAGTTGCCCA TCGGAGCCAG AGCAGCGAAG GCGTCAGTTC TCTCAGCAGC
    TCACCTTCCA ACAGCCTTGA GACACAGTCT CAGTCCCTCT CCCGTTCCCA GAGCATGGAT
    ATTGACGGTG TCTCCTGTGA GAAAAGCATG TCCCAGGTGG ATGTGGATTC GGGAATTGAG
    AATATGGAGG TGGACGAGAA CGACCGAAGA GAAAAACGGA GCCTGAGTGA CAAGGAGCCT
    TCTTCAGGTC CTGAGGTGTC TGAAGAGCAA GCCTTACAGC TGGTCTGTAA GATCTTCCGT
    GTCTCCTGGA AGGACCGCGA CAGAGATGTC ATCTTCCTTT CTTCTCTCTC TGCGCAGTTT
    AAACAGAACC CAAAAGAAGT GTTCTCTGAT TTTAAGGATC TGATTGGCCA GATTTTAATG
    GAAGTACTGA TGATGTCAAC TCAGACACGA GATGAAAATC CATTTGCCAG TCTGACAGCC
    ACATCCCAGC CCATCGCCAC GGCAGCTCGG TCACCAGACC GCAATCTCAT GCTGAACACT
    GGCTCCAGTT CAGGAACAAG CCCCATGTTC TGCAACATGG GCTCCTTCAG CACCAGCTCG
    TTGTCTAGCC TCTATGAAAC GAGCCCCGCC CCCTCTGCCA GTTTATGGAG TTTTGTGCCA
    GCGCTCAGCC CATCCTTTGC CTTACCTTCC CACCCAGCCC CACAGGCGGC TATGCCTTCC
    CAGTCCCTTA GCCCCCATGG TGTAGCCCCA CAGGCGGCTG TGCCTTCCCA GTCCCTCAGC
    CCCCATGGTG TAGCCCCGCA GGCGGCTGTG CCTTCCCAGT CCCTCAGCCC CCATGGTGTA
    GCCCCACAGG TGGCTGTGCC TTCCCAGTCC CTCAGCACCC ATGGTGTAGC CCCGCAGGCA
    GCTGTGCCTT CCCAGTCCCT CAGCACCCAT GGTGTAGCCC CGCAGGCGGC TGTGCCTTCC
    CAGTCCCTCA GCACCCATGG TGTAGCCCCG CAGGTGGCTG TGCCTTCCCA GTCCCTCAGC
    ACCCATGGTG TAGCCCCGCA GGCGGCTGTG CCTTCCCCAC CCCTCAGCCC TCATAGTACA
    GCTTCTGGAA ATGCTGCCGG AAGCCAGCCC TCATCCCCGA GGTATCGGCC CTATACTGTC
    ACGCACCCTT GGGGGTCTTC ACCTTGCCCC ACCCCACGAT CCTCCATTCT GGCTAGCTCC
    CCAGGCTTCC CTTCCCACGC AAGTAGTCCC CGGGCTGTAC CTGCCAGCTC CTCCAGACAG
    AGGCCCCGCA GCAGGGTCCC CGCCTTCCCA CCTGCCTCGC CCAGTGCCGC CAGCAGACGT
    CCCTCCTCCC TGCGGATCTC TCCTAGTTTG GGAGCCTCTG GTGGAGCAAG TAACTGGGAT
    TCGTACAGTG ACCATTTCAC CATTGAAACC TGCAAAGAGA CGGACATGCT GAACTACCTC
    ATCGAGTGTT TTGACCGAGT TGGAATAGAG GAAAAAAAAG CACCAAAGAT GTGCAGCCAG
    CCAGCAGTCA GCCAGCTTCT GAGCAACATC CGCTCGCAGT GCATCTCCCA CACCGCACTA
    GTGCTACAAG GTTCCCTCAC ACAGCCCAGG TCCTTGCAAC AGCCATCCTT CCTCGTGCCG
    TACATGCTCT GCAGGAACCT CCCGTACGGC TTCATTCAAG AGCTGGTGAG GACCACTCAC
    CAGGATGAAG AGGTGTTCAA GCAGATCTTT ATCCCCATTT TACAAGGCCT GGCGCTGGCT
    GCCAAAGAGT GCTCCTTGGA AAGCGACTAC TTTAAGTACC CCCTCATGGC ACTGGGTGAA
    CTCTGTGAAA CCAAGTTTGG GAAGACACAC CCTATGTGCA ATTTGGTCGC TTCTTTACCC
    CTGTGGTTGC CGAAGTCCTT AAGCCCTGGT TCTGGGCGGG AGCTGCAGAG ACTCTCCTAC
    TTAGGGGCCT TCTTTAGCTT CTCAGTCTTC GCAGAGGACG ATGCAAAGGT GGTTGAGAAA
    TACTTCTCAG GGCCTGCCAT TACCCTGGAG AACACACGCG TGGTCAGCCA GTCACTACAG
    CATTACCTGG AGCTGGGACG GCAAGAACTT TTCAAGATTC TACATAGTAT TTTGTTAAAT
    GGCGAAACTC GTGAAGCGGC TCTCAGCTAC ATGGCGGCCC TTGTCAATGC CAACATGAAG
    AAAGCCCAGA TGCAGGCAGA TGATCGGTTG GTGTCTACAG ACGGGTTTAT GCTGAATCTC
    CTTTGGGTCC TGCAGCAGCT AAGTACAAAA ATCAAGTTAG AAACAGTTGA CCCCACTTAC
    ATATTCCACC CAAGATGTCG GATCACTCTC CCGAATGATG AGACACGGAT AAATGCAACG
    ATGGAGGATG TGAATGAGCG GCTGACAGAG CTCTATGGTG ATCAGCCTCC ATTTTCTGAG
    CCAAAATTCC CTACGGAATG TTTCTTTCTC ACCCTGCATG CTCATCACCT CTCTATTCTG
    CCTAGTTGTC GTCGCTACAT TCGCCGACTT CGAGCCATCC GCGAGCTCAA TAGAACCGTG
    GAAGATTTGA AAAATAATGA AAGCCAGTGG AAAGATTCCC CACTGGCCAC CAGACACCGT
    GAAATGCTGA AGCGCTGTAA AACTCAGCTT AAGAAACTGG TACGGTGCAA GGCCTGTGCT
    GACGCTGGCT TACTTGACGA GAGCTTCCTA AGAAGATGCC TGAATTTTTA TGGCCTTCTC
    ATTCAGCTGA TGCTGCGCAT CCTGGACCCT GCATATCCTG ACGTAACACT GCCTTTGAAT
    TCAGAAGTCC CCAAGGTATT TGCAGCATTG CCTGAGTTTT ATGTCGAAGA TGTTGCTGAA
    TTTTTATTTT TTATTGTACA ATACTCTCCT CAGGTGCTTT ATGAGCCCTG CACTCAGGAC
    ATTGTCATGT TCCTCGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA CCCTTACCTA
    GTGGCCAAGC TGGTGGAGGT CATGTTCATG ACCAACCCTT CTGTCCAGCC GAGAACTCAG
    AAGTTCTTTG AGATGATTGA GAACCATCCT CTCTCTACCA AACTGCTGGT GCCGTCCCTC
    ATGAAGTTCT ATACAGATGT CGAGCATACT GGAGCCACCA GCGAGTTCTA TGACAAGTTC
    ACAATTCGCT ATCACATTAG CACTATTTTT AAAAGCCTTT GGCAAAACAT AGCTCACCAT
    GGCACCTTCA TGGAGGAGTT CAATTCTGGA AAGCAGTTCG TTCGGTATAT CAATATGCTA
    ATAAATGACA CGACCTTTTT ACTGGACGAA AGTCTGGAGT CTCTGAAGCG GATCCATGAA
    GTGCAAGAAG AGATGAAGAA CAAAGAACAG TGGGACCAGC TGCCTCGGGA TCAGCAGCAG
    GCCCGGCAGT CTCAGCTTGC TCAGGACGAG CGTGTTTCCC GTTCTTATCT TGCCCTGGCA
    ACTGAAACCG TGGACATGTT CCATCTCCTC ACCAAGCAAG TCCAGAAGCC GTTCCTGAGG
    CCAGAGCTTG GTCCCCGATT AGCAGCCATG CTGAACTTTA ACCTGCAGCA GCTCTGCGGA
    CCCAAGTGCC GGGACCTGAA AGTTGAAAAC CCAGAGAAGT ATGGTTTTGA GCCAAAGAAG
    CTGTTGGACC AACTGACAGA TATTTATTTA CAATTGGACT GTGCTCGCTT TGCTAAAGCC
    ATCGCCGATG ACCAGCGATC CTACAGCAAG GAACTGTTTG AAGAAGTCAT TTCAAAGATG
    CGGAAGGCGG GAATCAAATC CACCATCGCA ATAGAGAAGT TTAAACTTCT TGCAGAGAAA
    GTGGAGGAAA TCGTGGCAAA GAATGCGCGG GCAGAAATAG ACTACAGCGA TGCCCCGGAC
    GAGTTCAGAG ACCCTCTGAT GGACACCCTG ATGACCGATC CCGTGAGACT GCCCTCTGGC
    ACCGTCATGG ACCGTTCTAT CATCCTGCGG CATCTGCTCA ACTCCCCCAC CGACCCCTTC
    AACCGCCAGA TGCTGACTGA GAGCATGCTG GAGCCAGTGC CAGAGCTAAA GGAGCAGATT
    CAGGCCTGGA TGAGAGAGAA ACAGAGCAGT GACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006539152.1
    CDS209..4447
    Translation

    Target ORF information:

    RefSeq Version XM_006539089.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus ubiquitination factor E4B (Ube4b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006539089.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATTCGAC GGAGGCGCCT GGCACGACTT GCTGGTGGAC AGACCTCCCA GCCGACCACC 
    CCGCTTACAT CTCCCCAGAG GGAGAACCCT CCGGGACCTC CAATAGCTGC ATCAGCCCCA
    GGCCCCTCCC AGAGTCTTGG TCTCAATGTC CACAACATGA CCCCAGCTAC CTCCCCCATA
    GGTGCAGCAG GAGTTGCCCA TCGGAGCCAG AGCAGCGAAG GCGTCAGTTC TCTCAGCAGC
    TCACCTTCCA ACAGCCTTGA GACACAGTCT CAGTCCCTCT CCCGTTCCCA GAGCATGGAT
    ATTGACGGTG TCTCCTGTGA GAAAAGCATG TCCCAGGTGG ATGTGGATTC GGGAATTGAG
    AATATGGAGG TGGACGAGAA CGACCGAAGA GAAAAACGGA GCCTGAGTGA CAAGGAGCCT
    TCTTCAGGTC CTGAGGTGTC TGAAGAGCAA GCCTTACAGC TGGTCTGTAA GATCTTCCGT
    GTCTCCTGGA AGGACCGCGA CAGAGATGTC ATCTTCCTTT CTTCTCTCTC TGCGCAGTTT
    AAACAGAACC CAAAAGAAGT GTTCTCTGAT TTTAAGGATC TGATTGGCCA GATTTTAATG
    GAAGTACTGA TGATGTCAAC TCAGACACGA GATGAAAATC CATTTGCCAG TCTGACAGCC
    ACATCCCAGC CCATCGCCAC GGCAGCTCGG TCACCAGACC GCAATCTCAT GCTGAACACT
    GGCTCCAGTT CAGGAACAAG CCCCATGTTC TGCAACATGG GCTCCTTCAG CACCAGCTCG
    TTGTCTAGCC TCTATGAAAC GAGCCCCGCC CCCTCTGCCA GTTTATGGAG TTTTGTGCCA
    GCGCTCAGCC CATCCTTTGC CTTACCTTCC CACCCAGCCC CACAGGCGGC TATGCCTTCC
    CAGTCCCTTA GCCCCCATGG TGTAGCCCCA CAGGCGGCTG TGCCTTCCCA GTCCCTCAGC
    CCCCATGGTG TAGCCCCGCA GGCGGCTGTG CCTTCCCAGT CCCTCAGCCC CCATGGTGTA
    GCCCCACAGG TGGCTGTGCC TTCCCAGTCC CTCAGCACCC ATGGTGTAGC CCCGCAGGCA
    GCTGTGCCTT CCCAGTCCCT CAGCACCCAT GGTGTAGCCC CGCAGGCGGC TGTGCCTTCC
    CAGTCCCTCA GCACCCATGG TGTAGCCCCG CAGGTGGCTG TGCCTTCCCA GTCCCTCAGC
    ACCCATGGTG TAGCCCCGCA GGCGGCTGTG CCTTCCCCAC CCCTCAGCCC TCATAGTACA
    GCTTCTGGAA ATGCTGCCGG AAGCCAGCCC TCATCCCCGA GGTATCGGCC CTATACTGTC
    ACGCACCCTT GGGGGTCTTC ACCTTGCCCC ACCCCACGAT CCTCCATTCT GGCTAGCTCC
    CCAGGCTTCC CTTCCCACGC AAGTAGTCCC CGGGCTGTAC CTGCCAGCTC CTCCAGACAG
    AGGCCCCGCA GCAGGGTCCC CGCCTTCCCA CCTGCCTCGC CCAGTGCCGC CAGCAGACGT
    CCCTCCTCCC TGCGGATCTC TCCTAGTTTG GGAGCCTCTG GTGGAGCAAG TAACTGGGAT
    TCGTACAGTG ACCATTTCAC CATTGAAACC TGCAAAGAGA CGGACATGCT GAACTACCTC
    ATCGAGTGTT TTGACCGAGT TGGAATAGAG GAAAAAAAAG CACCAAAGAT GTGCAGCCAG
    CCAGCAGTCA GCCAGCTTCT GAGCAACATC CGCTCGCAGT GCATCTCCCA CACCGCACTA
    GTGCTACAAG GTTCCCTCAC ACAGCCCAGG TCCTTGCAAC AGCCATCCTT CCTCGTGCCG
    TACATGCTCT GCAGGAACCT CCCGTACGGC TTCATTCAAG AGCTGGTGAG GACCACTCAC
    CAGGATGAAG AGGTGTTCAA GCAGATCTTT ATCCCCATTT TACAAGGCCT GGCGCTGGCT
    GCCAAAGAGT GCTCCTTGGA AAGCGACTAC TTTAAGTACC CCCTCATGGC ACTGGGTGAA
    CTCTGTGAAA CCAAGTTTGG GAAGACACAC CCTATGTGCA ATTTGGTCGC TTCTTTACCC
    CTGTGGTTGC CGAAGTCCTT AAGCCCTGGT TCTGGGCGGG AGCTGCAGAG ACTCTCCTAC
    TTAGGGGCCT TCTTTAGCTT CTCAGTCTTC GCAGAGGACG ATGCAAAGGT GGTTGAGAAA
    TACTTCTCAG GGCCTGCCAT TACCCTGGAG AACACACGCG TGGTCAGCCA GTCACTACAG
    CATTACCTGG AGCTGGGACG GCAAGAACTT TTCAAGATTC TACATAGTAT TTTGTTAAAT
    GGCGAAACTC GTGAAGCGGC TCTCAGCTAC ATGGCGGCCC TTGTCAATGC CAACATGAAG
    AAAGCCCAGA TGCAGGCAGA TGATCGGTTG GTGTCTACAG ACGGGTTTAT GCTGAATCTC
    CTTTGGGTCC TGCAGCAGCT AAGTACAAAA ATCAAGTTAG AAACAGTTGA CCCCACTTAC
    ATATTCCACC CAAGATGTCG GATCACTCTC CCGAATGATG AGACACGGAT AAATGCAACG
    ATGGAGGATG TGAATGAGCG GCTGACAGAG CTCTATGGTG ATCAGCCTCC ATTTTCTGAG
    CCAAAATTCC CTACGGAATG TTTCTTTCTC ACCCTGCATG CTCATCACCT CTCTATTCTG
    CCTAGTTGTC GTCGCTACAT TCGCCGACTT CGAGCCATCC GCGAGCTCAA TAGAACCGTG
    GAAGATTTGA AAAATAATGA AAGCCAGTGG AAAGATTCCC CACTGGCCAC CAGACACCGT
    GAAATGCTGA AGCGCTGTAA AACTCAGCTT AAGAAACTGG TACGGTGCAA GGCCTGTGCT
    GACGCTGGCT TACTTGACGA GAGCTTCCTA AGAAGATGCC TGAATTTTTA TGGCCTTCTC
    ATTCAGCTGA TGCTGCGCAT CCTGGACCCT GCATATCCTG ACGTAACACT GCCTTTGAAT
    TCAGAAGTCC CCAAGGTATT TGCAGCATTG CCTGAGTTTT ATGTCGAAGA TGTTGCTGAA
    TTTTTATTTT TTATTGTACA ATACTCTCCT CAGGTGCTTT ATGAGCCCTG CACTCAGGAC
    ATTGTCATGT TCCTCGTTGT GATGCTGTGC AACCAGAACT ACATCCGAAA CCCTTACCTA
    GTGGCCAAGC TGGTGGAGGT CATGTTCATG ACCAACCCTT CTGTCCAGCC GAGAACTCAG
    AAGTTCTTTG AGATGATTGA GAACCATCCT CTCTCTACCA AACTGCTGGT GCCGTCCCTC
    ATGAAGTTCT ATACAGATGT CGAGCATACT GGAGCCACCA GCGAGTTCTA TGACAAGTTC
    ACAATTCGCT ATCACATTAG CACTATTTTT AAAAGCCTTT GGCAAAACAT AGCTCACCAT
    GGCACCTTCA TGGAGGAGTT CAATTCTGGA AAGCAGTTCG TTCGGTATAT CAATATGCTA
    ATAAATGACA CGACCTTTTT ACTGGACGAA AGTCTGGAGT CTCTGAAGCG GATCCATGAA
    GTGCAAGAAG AGATGAAGAA CAAAGAACAG TGGGACCAGC TGCCTCGGGA TCAGCAGCAG
    GCCCGGCAGT CTCAGCTTGC TCAGGACGAG CGTGTTTCCC GTTCTTATCT TGCCCTGGCA
    ACTGAAACCG TGGACATGTT CCATCTCCTC ACCAAGCAAG TCCAGAAGCC GTTCCTGAGG
    CCAGAGCTTG GTCCCCGATT AGCAGCCATG CTGAACTTTA ACCTGCAGCA GCTCTGCGGA
    CCCAAGTGCC GGGACCTGAA AGTTGAAAAC CCAGAGAAGT ATGGTTTTGA GCCAAAGAAG
    CTGTTGGACC AACTGACAGA TATTTATTTA CAATTGGACT GTGCTCGCTT TGCTAAAGCC
    ATCGCCGATG ACCAGCGATC CTACAGCAAG GAACTGTTTG AAGAAGTCAT TTCAAAGATG
    CGGAAGGCGG GAATCAAATC CACCATCGCA ATAGAGAAGT TTAAACTTCT TGCAGAGAAA
    GTGGAGGAAA TCGTGGCAAA GAATGCGCGG GCAGAAATAG ACTACAGCGA TGCCCCGGAC
    GAGTTCAGAG ACCCTCTGAT GGACACCCTG ATGACCGATC CCGTGAGACT GCCCTCTGGC
    ACCGTCATGG ACCGTTCTAT CATCCTGCGG CATCTGCTCA ACTCCCCCAC CGACCCCTTC
    AACCGCCAGA TGCTGACTGA GAGCATGCTG GAGCCAGTGC CAGAGCTAAA GGAGCAGATT
    CAGGCCTGGA TGAGAGAGAA ACAGAGCAGT GACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.