gRNAs
The following Ube4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu12578 | |
| Clone ID Related Accession (Same CDS sequence) | NM_022022.3 | |
| Accession Version | NM_022022.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-23 | |
| Organism | Mus musculus(house mouse) | |
| Product | ubiquitin conjugation factor E4 B | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK016689.1, BY279585.1 and BC075620.1. On or before Apr 5, 2008 this sequence version replaced XM_001477015.1, NM_022022.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF260926.1, BC067402.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_071305.2 |
| CDS | 727..4248 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_022022.3 |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu34371 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006539088.4 , XM_006539088.3 | |
| Accession Version | XM_006539088.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | ubiquitin conjugation factor E4 B isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539088.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006539151.1 |
| CDS | 2257..6516 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006539088.4 |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu34371 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006539088.4 , XM_006539088.3 | |
| Accession Version | XM_006539088.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | ubiquitin conjugation factor E4 B isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006539151.1 |
| CDS | 725..4984 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006539088.3 |
1 | ATGGAGGAGC TGAGCGCTGA CGAGATTCGA CGGAGGCGCC TGGCACGACT TGCTGGTGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu34372 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006539089.3 | |
| Accession Version | XM_006539089.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | ubiquitin conjugation factor E4 B isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATTCGAC GGAGGCGCCT GGCACGACTT GCTGGTGGAC AGACCTCCCA GCCGACCACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006539152.1 |
| CDS | 209..4447 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006539089.3 |
1 | ATGATTCGAC GGAGGCGCCT GGCACGACTT GCTGGTGGAC AGACCTCCCA GCCGACCACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis. |
Islet ?-, ?-, and ?-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor. |
A novel conserved isoform of the ubiquitin ligase UFD2a/UBE4B is expressed exclusively in mature striated muscle cells. |
Increased E4 activity in mice leads to ubiquitin-containing aggregates and degeneration of hypothalamic neurons resulting in obesity. |
WldS can delay Wallerian degeneration in mice when interaction with valosin-containing protein is weakened. |
