ORF » Species Summary » Mus musculus » Pramef20 cDNA ORF clone

Pramef20 cDNA ORF clone, Mus musculus(house mouse)

The following Pramef20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pramef20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu05398 NM_001115077.1
Latest version!		 Mus musculus PRAME family member 20 (Pramef20), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Pramef20 ( NM_001115077.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001108549.1 Mus musculus PRAME family member 20 (Pramef20), mRNA.
    Price & Availability↑
    CloneID OMu05398
    Clone ID Related Accession (Same CDS sequence) NM_001115077.1
    Accession Version NM_001115077.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-25
    Organism Mus musculus(house mouse)
    Product novel preferentially expressed antigen in melanoma (Prame) protein
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AL606926.6. On Mar 2, 2008 this sequence version replaced XM_891640.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGCAGCA AGCCCTTACT CACACTCCAA GAGCTGGCAG CCCAGAACAT TCTGAGGAAT 
    GAGGCTTTGG CCATCAGCGC TTTGCAGTAC TTACCTGAAC TATTCTTACC ACAACTGTTG 
    AAGCAAGCCT ACGATGGTAA CCATCTTAAG GTCCTGAGGG CAATAGTATC TTCCTGGCCT 
    TTCCCTCGCC TTCCTCTTGG AGCTCTGAAG AAGAGAACAC CCTACTTGGA GACTCAATTA 
    CAAGTTGTCT TAGAGGAGGT TGATAAGCTG CTTATCCAGG AGGTTCACCC CAGGGAGTAC 
    AAACTAGAAG TGCTGGATTT ACGGTCTGTG GGACAAAACT ATTTGAATGT GTGGCCTGGG 
    GCCATGGATG ACTGGTTACC CAAGACCACT CAAACAGATC CTTGCTGTTC TAAGACAGTA 
    ATGACACAGC CCTTGAAAGT ACTAGTGGAC TTGCAGCTCA ACAATAGAGG CCAGAGTAGG 
    CTGTTCTCCT ACCTCGTTCA GTGGAGTGAT AGGAGGAAAG GGTTGTTACA GCTGTACTGT 
    AATAAGCTTC AGATCTGGTC ACCATCCCAA CAAAACTACA GGAAGCTCTC ACAAAAGGTG 
    AACCTGGACT ATGTGGAAAC ACTGGGCCTG CATTCCTCCT GCAGTCCATC CTTCTTGCTT 
    AACTTTGCTC CTTACTTGCG CCAAATGAGG AACTTGCGTA AACTTGCTCT CTCCAACATC 
    CGTGAAGAAC ACATTATCTC TCCGGAGAAG AGGAGGTGTA TCATCACCAG GTTCACCTTG 
    CAGATCCTTA AGCTGGAGAG CCTCCAGAAG CTGCATCTAG ATGCTGTCTG CTTCCTTGAG 
    GGACACCTAG ACCAGCTGCT TGGGTCCGTG AAGACCTCCT TGGATGAACT GGCAGTGACT 
    CACTGTAATC TCTCAGTATC AGAATGGAGC CATTTTTCTG AGTTCCCATG TGTCAGTCAG 
    TTGCAACAAC TGAATTTGGA AAATGTCAGA TTGACTGGTT TAAGTCCTGA ACCTCTCCAA 
    GTTCTGTTAG TAAAAGCTGG ACCCACCCTG GTGGCCTTGG ACTTGGAGGA CTGTCACCTT 
    GAAGATGGCC AGCTCTATGC CATCCTACCT GCCCTGAGCA AGTGCTTCAA GCTCACCAAG 
    TTCAGTTTCT ATGGGAACCA AATCTCTATG CTTGCCATGA AGGACCTACT ACACCACACT 
    GCCAGCTTGA TACATCTAAG GCTGGAGCTA TACCCTGTTC CTCAGGACAG TTATGACTAC 
    CATGGTACTC CCCTCATGCA AAGGATGCAA CAACATTGTG ATGAGCTTAT GAATCTACTG 
    AAAACATTTA GGAATCCAGG CAGAGTCTTC TTCGGTACTG ACCGCTGTGA TCAGTGCTGC 
    AATAGATATA TCTATAACAA AACCATTATG TGTAATTGTC GGAGATCTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001108549.1
    CDS1..1434
    Translation

    Target ORF information:

    RefSeq Version NM_001115077.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus PRAME family member 20 (Pramef20), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001115077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGCAGCA AGCCCTTACT CACACTCCAA GAGCTGGCAG CCCAGAACAT TCTGAGGAAT 
    GAGGCTTTGG CCATCAGCGC TTTGCAGTAC TTACCTGAAC TATTCTTACC ACAACTGTTG 
    AAGCAAGCCT ACGATGGTAA CCATCTTAAG GTCCTGAGGG CAATAGTATC TTCCTGGCCT 
    TTCCCTCGCC TTCCTCTTGG AGCTCTGAAG AAGAGAACAC CCTACTTGGA GACTCAATTA 
    CAAGTTGTCT TAGAGGAGGT TGATAAGCTG CTTATCCAGG AGGTTCACCC CAGGGAGTAC 
    AAACTAGAAG TGCTGGATTT ACGGTCTGTG GGACAAAACT ATTTGAATGT GTGGCCTGGG 
    GCCATGGATG ACTGGTTACC CAAGACCACT CAAACAGATC CTTGCTGTTC TAAGACAGTA 
    ATGACACAGC CCTTGAAAGT ACTAGTGGAC TTGCAGCTCA ACAATAGAGG CCAGAGTAGG 
    CTGTTCTCCT ACCTCGTTCA GTGGAGTGAT AGGAGGAAAG GGTTGTTACA GCTGTACTGT 
    AATAAGCTTC AGATCTGGTC ACCATCCCAA CAAAACTACA GGAAGCTCTC ACAAAAGGTG 
    AACCTGGACT ATGTGGAAAC ACTGGGCCTG CATTCCTCCT GCAGTCCATC CTTCTTGCTT 
    AACTTTGCTC CTTACTTGCG CCAAATGAGG AACTTGCGTA AACTTGCTCT CTCCAACATC 
    CGTGAAGAAC ACATTATCTC TCCGGAGAAG AGGAGGTGTA TCATCACCAG GTTCACCTTG 
    CAGATCCTTA AGCTGGAGAG CCTCCAGAAG CTGCATCTAG ATGCTGTCTG CTTCCTTGAG 
    GGACACCTAG ACCAGCTGCT TGGGTCCGTG AAGACCTCCT TGGATGAACT GGCAGTGACT 
    CACTGTAATC TCTCAGTATC AGAATGGAGC CATTTTTCTG AGTTCCCATG TGTCAGTCAG 
    TTGCAACAAC TGAATTTGGA AAATGTCAGA TTGACTGGTT TAAGTCCTGA ACCTCTCCAA 
    GTTCTGTTAG TAAAAGCTGG ACCCACCCTG GTGGCCTTGG ACTTGGAGGA CTGTCACCTT 
    GAAGATGGCC AGCTCTATGC CATCCTACCT GCCCTGAGCA AGTGCTTCAA GCTCACCAAG 
    TTCAGTTTCT ATGGGAACCA AATCTCTATG CTTGCCATGA AGGACCTACT ACACCACACT 
    GCCAGCTTGA TACATCTAAG GCTGGAGCTA TACCCTGTTC CTCAGGACAG TTATGACTAC 
    CATGGTACTC CCCTCATGCA AAGGATGCAA CAACATTGTG ATGAGCTTAT GAATCTACTG 
    AAAACATTTA GGAATCCAGG CAGAGTCTTC TTCGGTACTG ACCGCTGTGA TCAGTGCTGC 
    AATAGATATA TCTATAACAA AACCATTATG TGTAATTGTC GGAGATCTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    High-throughput discovery of novel developmental phenotypes.
    Nature537(7621)508-514(2016 09)
    Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA


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