GUCY1A3 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY1A3
Entrez Gene ID	60596
Full Name	guanylate cyclase 1, soluble, alpha 1
Synonyms	1200016O07Rik,GCS-alpha-1,GCS-alpha-3,Gucy1a3,sGC-alpha1
General protein information	Preferred Names guanylate cyclase 1, soluble, alpha 1 Names guanylate cyclase soluble subunit alpha-3 guanylate cyclase 1, soluble, alpha 3 soluble guanylate cyclase large subunit
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 3 Map Location: 3\|3 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_021896.5	NP_068696.2	guanylate cyclase soluble subunit alpha-3
XM_006501843.3	XP_006501906.1	guanylate cyclase soluble subunit alpha-3 isoform X1
XM_011240184.1	XP_011238486.1	guanylate cyclase soluble subunit alpha-3 isoform X1
NM_001356988.1	NP_001343917.1	guanylate cyclase soluble subunit alpha-1
NM_001356987.1	NP_001343916.1	guanylate cyclase soluble subunit alpha-1
NM_021896.6	NP_068696.2	guanylate cyclase soluble subunit alpha-1
XM_006501843.2	XP_006501906.1	guanylate cyclase soluble subunit alpha-3 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gucy1a3	XP_004911212.1
Canis lupus familiaris (dog)	GUCY1A3	NP_001018045.1
Bos taurus (cattle)	GUCY1A3	NP_786972.1
Homo sapiens (human)	GUCY1A3	NP_001124154.1
Mus musculus (house mouse)	Gucy1a3	NP_068696.2
Rattus norvegicus (Norway rat)	Gucy1a3	NP_058786.2
Pan troglodytes (chimpanzee)	GUCY1A3	XP_003310599.1
	GUCY1A3	XP_002804280.1
Gallus gallus (chicken)	GUCY1A3	XP_420375.1
Danio rerio (zebrafish)	gucy1a3	NP_001017725.1

Gene Ontology
Bibliography

Related articles in PubMed

The Ability of Nitric Oxide to Lower Intraocular Pressure Is Dependent on Guanylyl Cyclase.
Muenster S, Lieb WS, Fabry G, Allen KN, Kamat SS, Guy AH, Dordea AC, Teixeira L, Tainsh RE, Yu B, Zhu W, Ashpole NE, Malhotra R, Brouckaert P, Bloch DB, Scherrer-Crosbie M, Stamer WD, Kuehn MH, Pasquale LR, Buys ES
Investigative ophthalmology & visual science58(11)4826-4835(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data demonstrate that a reduction in cardiac sGC activity worsens doxorubicin-induced cardiotoxicity in mice and identify sGC as a potential therapeutic target.
Title: Decreased Soluble Guanylate Cyclase Contributes to Cardiac Dysfunction Induced by Chronic Doxorubicin Treatment in Mice.

Exogenous or paracrine sources of NO promote the specification towards the myocyte lineage and expression of cardiac sarcomeric proteins of adult cardiac progenitor cells. This is contingent upon the expression and activity of the alpha1 subunit of guanylyl cyclase in CPC that is necessary for NO-mediated inhibition of the canonical Wnt/beta-catenin pathway.
Title: Paracrine nitric oxide induces expression of cardiac sarcomeric proteins in adult progenitor cells through soluble guanylyl cyclase/cyclic-guanosine monophosphate and Wnt/?-catenin inhibition.

We suggest that Gucy1a3 activity promotes the phenotypic switching of smooth muscle cells from a contractile to a synthetic state, fostering the formation of atherosclerosis.
Title: Proatherosclerotic Effect of the ?1-Subunit of Soluble Guanylyl Cyclase by Promoting Smooth Muscle Phenotypic Switching.

The authors elucidated this dependency by showing that guanylate cyclase-1 is a novel rhodopsin-binding protein.
Title: Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking.

Cellular markers revealed that vascular SMC differentiation decreased, whereas myofibroblast activation increased in the hyperoxic sGC-alpha1 KO pup lung.
Title: Soluble guanylate cyclase modulates alveolarization in the newborn lung.

The following GUCY1A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY1A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20147	NM_021896.6 Latest version!	Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu20147	NM_001356987.1 Latest version!	Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu20147	NM_001356988.1 Latest version!	Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu20147	XM_006501843.3 Latest version!	Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu20147	XM_011240184.1 Latest version!	Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
View Old Accession Versions >>
OMu20147	NM_021896.5	Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu20147	XM_006501843.2	Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY1A3 ( NM_021896.5 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_068696.2 Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), mRNA.

Price & Availability↑

CloneID	OMu20147
Clone ID Related Accession (Same CDS sequence)	NM_021896.5 , XM_006501843.3 , XM_011240184.1 , NM_001356988.1 , NM_001356987.1 , NM_021896.6 , XM_006501843.2
Accession Version	NM_021896.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-06
Organism	Mus musculus(House mouse)
Product	guanylate cyclase soluble subunit alpha-3
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY150724.1, AK004815.1, BC057327.1 and CO042590.1. On Jun 18, 2009 this sequence version replaced NM_021896.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.76645.1, SRR1660819.63091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068696.2
CDS	311..2386
Translation	MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLPICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKIEENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_021896.5
Organism	Mus musculus(House mouse)
Definition	Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021896.5

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A3 ( NM_001356988.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001343917.1 Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20147
Clone ID Related Accession (Same CDS sequence)	NM_021896.5 , XM_006501843.3 , XM_011240184.1 , NM_001356988.1 , NM_001356987.1 , NM_021896.6 , XM_006501843.2
Accession Version	NM_001356988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	guanylate cyclase soluble subunit alpha-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121563.3 and CO042590.1. On Oct 14, 2017 this sequence version replaced XM_011240184.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.96994.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343917.1
CDS	493..2568
Translation	MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLPICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKIEENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001356988.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356988.1

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A3 ( NM_001356987.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001343916.1 Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 3, mRNA.

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CloneID	OMu20147
Clone ID Related Accession (Same CDS sequence)	NM_021896.5 , XM_006501843.3 , XM_011240184.1 , NM_001356988.1 , NM_001356987.1 , NM_021896.6 , XM_006501843.2
Accession Version	NM_001356987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	guanylate cyclase soluble subunit alpha-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC094310.1, AC121563.3 and CO042590.1. On Oct 14, 2017 this sequence version replaced XM_006501843.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094310.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343916.1
CDS	367..2442
Translation	MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLPICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKIEENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_001356987.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356987.1

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A3 ( NM_021896.6 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_068696.2 Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 1, mRNA.

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CloneID	OMu20147
Clone ID Related Accession (Same CDS sequence)	NM_021896.5 , XM_006501843.3 , XM_011240184.1 , NM_001356988.1 , NM_001356987.1 , NM_021896.6 , XM_006501843.2
Accession Version	NM_021896.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	guanylate cyclase soluble subunit alpha-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121563.3, BY150724.1, AK004815.1, BC057327.1 and CO042590.1. On Oct 14, 2017 this sequence version replaced NM_021896.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.63091.1, SRR1660821.76645.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068696.2
CDS	380..2455
Translation	MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLPICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKIEENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	NM_021896.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanylate cyclase 1, soluble, alpha 1 (Gucy1a1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021896.6

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A3 ( XM_006501843.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006501906.1 Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X2, mRNA.

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CloneID	OMu20147
Clone ID Related Accession (Same CDS sequence)	NM_021896.5 , XM_006501843.3 , XM_011240184.1 , NM_001356988.1 , NM_001356987.1 , NM_021896.6 , XM_006501843.2
Accession Version	XM_006501843.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501906.1
CDS	515..2590
Translation	MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLPICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKIEENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_006501843.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501843.3

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A3 ( XM_011240184.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011238486.1 Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X1, mRNA.

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CloneID	OMu20147
Clone ID Related Accession (Same CDS sequence)	NM_021896.5 , XM_006501843.3 , XM_011240184.1 , NM_001356988.1 , NM_001356987.1 , NM_021896.6 , XM_006501843.2
Accession Version	XM_011240184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238486.1
CDS	493..2568
Translation	MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLPICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKIEENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_011240184.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240184.1

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY1A3 ( XM_006501843.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006501906.1 Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu20147
Clone ID Related Accession (Same CDS sequence)	NM_021896.5 , XM_006501843.3 , XM_011240184.1 , NM_001356988.1 , NM_001356987.1 , NM_021896.6 , XM_006501843.2
Accession Version	XM_006501843.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006501906.1

Translation

MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLPICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKIEENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGID

Target ORF information:

RefSeq Version	XM_006501843.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus guanylate cyclase 1, soluble, alpha 3 (Gucy1a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501843.2

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA GGATCTCAAG ATCACAGGGG AGTGTCCTTT CTCCTTACTG 
GCCCCTGGTC AGGTTCCTAA GGAGCCAACA GAGGAGGTGG CTGGAGGCTC TGAGGGCTGC 
CAGGCTACTC TGCCCATCTG CCAGTACTTT CCTGAGAAGA ATGCAGAAGG GAGTCTCCCC 
CAAAGAAAGA CAAGCCGCAA CAGAGTCTAC CTGCACACCC TGGCAGAGAG TATTTGCAAG 
CTCATCTTCC CAGAGTGTGA GCGACTGAAC CTTGCACTTC AGAGAACCTT GGCAAAGCAT 
AAAATAGAAG AAAACAGGAA ATCTTCAGAA AAAGAAGACC TTGAAAAAAT AATCGCAGAA 
GAAGCAATTG CAGCAGGTGC CCCAGTGGAG GCGCTCAAAG ACTCTCTGGG CGAGGAGCTG 
TTCAAGATCT GCTATGAGGA AGATGAGCAC ATTTTGGGCG TGGTTGGCGG CACCCTGAAG 
GACTTTCTAA ATAGCTTCAG CACGCTCCTC AAGCAGAGCA GCCACTGCCA AGAGGCGGAG 
AGGCGGGGAC GACTGGAAGA TGCCTCCATC TTATGCCTGG ACAAGGACCA GGACTTTCTA 
AATGTTTACT ACTTCTTCCC GAAGAGAACC ACAGCCCTGC TTCTCCCTGG TATCATTAAA 
GCGGCTGCTC GCATACTGTA CGAAAGCCAC GTGGAGGTGT CCCTGATGCC TCCCTGCTTC 
CGAAGTGACT GTACCGAGTT TGTGAACCAG CCCTATTTGC TCTACTCCGT TCATGTGAAG 
AGCACCAAGC CGTCCCTGTC CCCAGGCAAG CCCCAGTCCT CGCTAGTGAT CCCCGCTTCG 
CTCTTCTGCA AGACTTTCCC GTTCCATTTC ATGCTGGACC GAGACCTGGC CATCCTGCAG 
CTGGGTAACG GCATCAGAAG GCTGGTGAAC AAGAGGGACT TCCAAGGGAA GCCCAACTTT 
GAAGAGTTCT TTGAAATTCT AACTCCCAAA ATCAACCAGA CATTTAGTGG CATCATGACA 
ATGTTGAACA TGCAGTTTGT CATCCGGGTG AGGAGATGGG ATAACTCGGT GAAGAAATCG 
TCAAGGGTTA TGGATCTCAA AGGTCAAATG ATCTACATCG TTGAATCCAG TGCCATCTTG 
TTCTTAGGGT CACCATGTGT GGACAGGCTG GAAGATTTCA CAGGACGGGG GCTCTATCTG 
TCCGACATCC CAATTCATAA CGCCCTGAGG GATGTTGTCT TGATAGGGGA GCAGGCACGG 
GCTCAAGATG GCCTCAAGAA GAGGTTGGGG AAGCTGAAGG CAACCCTGGA GCATGCCCAC 
CAAGCCCTGG AGGAGGAGAA GAAGAGGACA GTGGATCTGC TGTGCTCTAT CTTCCCCTCT 
GAGGTTGCTC AGCAGCTGTG GCAAGGACAA ATTGTGCAAG CCAAGAAATT CAGCGAGGTC 
ACCATGCTTT TCTCAGATAT CGTAGGGTTC ACTGCTATCT GCTCTCAGTG TTCACCTCTG 
CAGGTCATCA CGATGCTCAA CGCTCTCTAC ACTCGCTTTG ACCAGCAGTG TGGAGAGCTG 
GATGTCTACA AGGTGGAGAC CATCGGGGAT GCATATTGTG TGGCAGGTGG ATTGCACAGA 
GAGAGTGACA CCCATGCTGT CCAGATAGCA CTGATGGCCC TGAAGATGAT GGAGCTCTCC 
AATGAGGTCA TGTCTCCCCA CGGAGAACCT ATCAAGATGC GAATTGGACT ACATTCTGGA 
TCAGTGTTTG CTGGAGTTGT CGGAGTGAAG ATGCCCCGGT ATTGCCTGTT TGGAAACAAT 
GTCACTCTGG CTAACAAATT TGAATCCTGC AGTGTGCCTC GGAAAATCAA TGTCAGCCCC 
ACCACATACA GGTTACTCAA AGACTGTCCT GGCTTTGTGT TCACCCCGAG ATCAAGGGAG 
GAGCTTCCAC CAAACTTCCC TAGTGACATT CCTGGGATCT GTCACTTTCT GGATGCTTAT 
CACCATCAAG GACCTAATTC CAAACCATGG TTCCAGGATA AAGATGTGGA AGATGGAAAC 
GCCAACTTCT TAGGCAAAGC GTCAGGGATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Ability of Nitric Oxide to Lower Intraocular Pressure Is Dependent on Guanylyl Cyclase.
Investigative ophthalmology & visual science58(11)4826-4835(2017 Sep)
Muenster S,Lieb WS,Fabry G,Allen KN,Kamat SS,Guy AH,Dordea AC,Teixeira L,Tainsh RE,Yu B,Zhu W,Ashpole NE,Malhotra R,Brouckaert P,Bloch DB,Scherrer-Crosbie M,Stamer WD,Kuehn MH,Pasquale LR,Buys ES

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GUCY1A3 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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