Acss2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acss2
Entrez Gene ID	60525
Full Name	acyl-CoA synthetase short-chain family member 2
Synonyms	1110017C11Rik,ACAS,Acas1,Acas2,AceCS1,Acs1
General protein information	Preferred Names acyl-CoA synthetase short-chain family member 2 Names acetyl-coenzyme A synthetase, cytoplasmic ACS aceCS acetate--CoA ligase acetyl-CoA synthetase 1 acetyl-Coenzyme A synthetase 1 (AMP forming) acetyl-Coenzyme A synthetase 2 (ADP forming) acetyl-Coenzyme A synthetase 2 (AMP forming) acyl-activating enzyme
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H1

mRNA and Protein(s)

mRNA	Protein	Name
NM_019811.3	NP_062785.2	acetyl-coenzyme A synthetase, cytoplasmic
XM_006499973.1	XP_006500036.1	acetyl-coenzyme A synthetase, cytoplasmic isoform X1
XM_006499974.1	XP_006500037.1	acetyl-coenzyme A synthetase, cytoplasmic isoform X3
XM_006499975.1	XP_006500038.1	acetyl-coenzyme A synthetase, cytoplasmic isoform X4
XM_017319156.1	XP_017174645.1	acetyl-coenzyme A synthetase, cytoplasmic isoform X2

Pathways from BioSystems

Homologies

	ACSS2	XP_001103094.1
Canis lupus familiaris (dog)	ACSS2	XP_534395.4
Mus musculus (house mouse)	Acss2	NP_062785.2
Rattus norvegicus (Norway rat)	Acss2	NP_001101263.1
Caenorhabditis elegans (roundworm)	acs-19	NP_001021205.1
Arabidopsis thaliana (thale cress)	ACS	NP_001031974.2
Pan troglodytes (chimpanzee)	ACSS2	XP_514806.3
Oryza sativa (rice)	Os02g0525900	NP_001046995.1
Bos taurus (cattle)	ACSS2	NP_001098809.1
Drosophila melanogaster (fruit fly)	AcCoAS	NP_001014599.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006569	XP_316594.2
Xenopus tropicalis (tropical clawed frog)	acss2	XP_004918555.1
Homo sapiens (human)	ACSS2	NP_001070020.2
Gallus gallus (chicken)	ACSS2	XP_417342.4

Gene Ontology
Bibliography

Related articles in PubMed

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.
Mews P, Donahue G, Drake AM, Luczak V, Abel T, Berger SL
Nature546(7658)381-386(2017 06)

Possible involvement of ACSS2 gene in alcoholism.
Ribeiro AF, de Lacerda RB, Correia D, Brunialti-Godard AL, de Miranda DM, Campos VR, de Souza VF, Ribeiro AM
Journal of neural transmission (Vienna, Austria : 1996)(2017 May)

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.
Chen L, Chen Q, Xie B, Quan C, Sheng Y, Zhu S, Rong P, Zhou S, Sakamoto K, MacKintosh C, Wang HY, Chen S
Proceedings of the National Academy of Sciences of the United States of America113(26)7219-24(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results reveal a connection between cellular metabolism, gene regulation, and neural plasticity and establish a link between acetyl-CoA generation 'on-site' at chromatin for histone acetylation and the transcription of key neuronal genes.
Title: Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.

Study revealed that the activity of acetyl-CoA synthetase 2 (ACSS2) contributes to cancer cell growth under low-oxygen and lipid-depleted conditions.
Title: Acetyl-CoA synthetase 2 promotes acetate utilization and maintains cancer cell growth under metabolic stress.

Study shows that ACSS2 is upregulated in the human orthotopic tumor and primary human tumors, as well as a murine glioma model; the tumors do not oxidize [U-(13)C]glutamine. In vivo oxidation of [1,2-(13)C]acetate was validated in brain tumor patients and was correlated with expression of acetyl-CoA synthetase enzyme 2, ACSS2.
Title: Acetate is a bioenergetic substrate for human glioblastoma and brain metastases.

Study reports evidence that the nucleocytosolic ACSS2 enzyme is of critical importance for mammalian cells to utilize acetate as a source of acetyl-CoA, and that mice lacking this enzyme exhibit a substantial reduction in tumor burden in two genetic models of liver cancer.
Title: Acetate dependence of tumors.

Acss2 generates a specific pool of acetyl CoA during hypoxia that modulates interactions of the acetylase/coactivator Cbp (also known as Crebbp) with the stress-responsive transcription factor, Hif-2. Because erythropoietin is a Hif-2 target gene, red blood cell production in mice is also regulated by Acss2. Recovery from anemia is accelerated by administering acetate, although this requires the presence of Acss2.
Title: An acetate switch regulates stress erythropoiesis.

The following Acss2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acss2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30751	XM_006499974.1 Latest version!	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu30752	XM_006499975.1 Latest version!	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu30750	XM_006499973.1 Latest version!	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu11363	NM_019811.3 Latest version!	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$412.30 $589.00
OMu80355	XM_017319156.1 Latest version!	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acss2 ( XM_006499974.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500037.1 Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu30751
Clone ID Related Accession (Same CDS sequence)	XM_006499974.1
Accession Version	XM_006499974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	acetyl-coenzyme A synthetase, cytoplasmic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGAG CAACTACAAA CATCTGCTAC AACGTGCTGG ATCGAAATGT CCATGAGAAA 
AAACTTGGCG ACAAAGTTGC TTTTTACTGG GAGGGCAATG AGCCAGGGGA GACCACCAAG 
ATCACATACC GTGAACTCCT GGTCCAGGTG TGTCAGTTCA GCAATGTTCT CCGTAAACAG 
GGCATTCAGA AGGGTGACCG AGTGGCCATC TACATGCCTA TGATCTTGGA ACTTGTGGTG 
GCTATGCTGG CATGCGCTCG CCTTGGAGCT TTGCACTCCA TTGTGTTTGC AGGCTTCTCT 
GCAGAGTCTC TCTGTGAAAG GATCTTGGAT TCCAGTTGCT GCCTGCTCAT CACTACAGAT 
GCCTTCTACA GGGGGGAAAA ACTTGTGAAC CTGAAGGAGC TGGCTGATGA GTCCTTGGAG 
AAGTGCCGAG AGAAGGGCTT CCCAGTGAGA TGCTGCATTG TGGTCAAACA TCTGGGGCGG 
GCAGAGCTGG GCATGAATGA CTCCCCCAGC CAGTCCCCAC CAGTTAAGAG GCCATGTCCA 
GATGTCCAGG GTAAGCTGAG AGAGAAATCC AAGTGCATCC GACCCCAGAT CTGCTGGAAC 
GAAGGGGTTG ACTTATGGTG GCATGAACTC ATGCAGCAGG CAGGAGACGA GTGTGAGCCT 
GAGTGGTGTG ATGCTGAGGA CCCACTCTTC ATCTTGTACA CCAGTGGCTC CACAGGCAAA 
CCTAAGGGTG TGGTGCACAC AATTGGAGGC TATATGCTCT ATGTGGCTAC AACTTTCAAG 
TATGTGTTTG ATTTCCACCC GGAAGATGTG TTCTGGTGCA CAGCAGACAT CGGCTGGATC 
ACTGGTCATT CCTATGTCAC CTATGGGCCA CTGGCTAATG GTGCCACTAG TGTTTTGTTT 
GAGGGGATCC CCACATACCC AGATGAAGGG CGCTTGTGGA GCATTGTGGA CAAATACAAG 
GTGACCAAGT TCTACACGGC ACCAACAGCC ATCCGGATGC TCATGAAGTT TGGAGATGAT 
CCTGTCACCA AGCATAGCCG GGCATCCCTG CAGGTGCTGG GCACAGTAGG TGAACCCATC 
AACCCTGAAG CCTGGCTATG GTACCACCGG GTAGTAGGTT CCCAGCGCTG CCCCATTGTA 
GACACCTTCT GGCAAACAGA AACAGGTGGC CATATGCTGA CCCCTCTCCC TGGCGCCACA 
CCCATGAAAC CTGGTTCTGC TTCTTTCCCA TTCTTCGGTG TAGCGCCTGC AATCCTGAAT 
GAGTCCGGGG AGGAGCTGGA AGGGGAAGCT GAAGGTTATC TGGTGTTCAA GCAGCCCTGG 
CCAGGGATCA TGCGCACAGT CTATGGGAAC CACACACGGT TTGAGACCAC CTACTTTAAG 
AAGTTCCCTG GCTACTATGT GACCGGAGAT GGCTGCCGAC GGGATCAGGA TGGCTATTAC 
TGGATCACGG GCAGGATTGA TGACATGCTC AATGTGTCTG GACATCTCCT GAGTACAGCA 
GAGGTGGAAT CGGCACTTGT GGAACACGAG GCTGTCGCAG AGGCAGCTGT GGTGGGCCAC 
CCTCATCCTG TGAAGGGCGA ATGCCTCTAC TGCTTTGTTA CCTTGTGTGA TGGCCATACC 
TTCAGCCCCA CACTCACAGA GGAACTCAAG AAGCAGATTA GAGAAAAGAT TGGCCCCATT 
GCCACACCAG ACTACATCCA GAATGCACCT GGCTTGCCTA AAACACGCTC AGGGAAAATC 
ATGAGGCGAG TTCTCCGGAA GATTGCTCAG AATGACCATG ACCTGGGGGA TACATCTACG 
GTGGCTGACC CATCTGTCAT CAACCATCTC TTCAGTCACC GCTGCCTGAC CACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500037.1
CDS	317..2176
Translation	MKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQGKLREKSKCIRPQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTTQ

Target ORF information:

RefSeq Version	XM_006499974.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499974.1

ORF Insert Sequence:

ATGAAAGGAG CAACTACAAA CATCTGCTAC AACGTGCTGG ATCGAAATGT CCATGAGAAA 
AAACTTGGCG ACAAAGTTGC TTTTTACTGG GAGGGCAATG AGCCAGGGGA GACCACCAAG 
ATCACATACC GTGAACTCCT GGTCCAGGTG TGTCAGTTCA GCAATGTTCT CCGTAAACAG 
GGCATTCAGA AGGGTGACCG AGTGGCCATC TACATGCCTA TGATCTTGGA ACTTGTGGTG 
GCTATGCTGG CATGCGCTCG CCTTGGAGCT TTGCACTCCA TTGTGTTTGC AGGCTTCTCT 
GCAGAGTCTC TCTGTGAAAG GATCTTGGAT TCCAGTTGCT GCCTGCTCAT CACTACAGAT 
GCCTTCTACA GGGGGGAAAA ACTTGTGAAC CTGAAGGAGC TGGCTGATGA GTCCTTGGAG 
AAGTGCCGAG AGAAGGGCTT CCCAGTGAGA TGCTGCATTG TGGTCAAACA TCTGGGGCGG 
GCAGAGCTGG GCATGAATGA CTCCCCCAGC CAGTCCCCAC CAGTTAAGAG GCCATGTCCA 
GATGTCCAGG GTAAGCTGAG AGAGAAATCC AAGTGCATCC GACCCCAGAT CTGCTGGAAC 
GAAGGGGTTG ACTTATGGTG GCATGAACTC ATGCAGCAGG CAGGAGACGA GTGTGAGCCT 
GAGTGGTGTG ATGCTGAGGA CCCACTCTTC ATCTTGTACA CCAGTGGCTC CACAGGCAAA 
CCTAAGGGTG TGGTGCACAC AATTGGAGGC TATATGCTCT ATGTGGCTAC AACTTTCAAG 
TATGTGTTTG ATTTCCACCC GGAAGATGTG TTCTGGTGCA CAGCAGACAT CGGCTGGATC 
ACTGGTCATT CCTATGTCAC CTATGGGCCA CTGGCTAATG GTGCCACTAG TGTTTTGTTT 
GAGGGGATCC CCACATACCC AGATGAAGGG CGCTTGTGGA GCATTGTGGA CAAATACAAG 
GTGACCAAGT TCTACACGGC ACCAACAGCC ATCCGGATGC TCATGAAGTT TGGAGATGAT 
CCTGTCACCA AGCATAGCCG GGCATCCCTG CAGGTGCTGG GCACAGTAGG TGAACCCATC 
AACCCTGAAG CCTGGCTATG GTACCACCGG GTAGTAGGTT CCCAGCGCTG CCCCATTGTA 
GACACCTTCT GGCAAACAGA AACAGGTGGC CATATGCTGA CCCCTCTCCC TGGCGCCACA 
CCCATGAAAC CTGGTTCTGC TTCTTTCCCA TTCTTCGGTG TAGCGCCTGC AATCCTGAAT 
GAGTCCGGGG AGGAGCTGGA AGGGGAAGCT GAAGGTTATC TGGTGTTCAA GCAGCCCTGG 
CCAGGGATCA TGCGCACAGT CTATGGGAAC CACACACGGT TTGAGACCAC CTACTTTAAG 
AAGTTCCCTG GCTACTATGT GACCGGAGAT GGCTGCCGAC GGGATCAGGA TGGCTATTAC 
TGGATCACGG GCAGGATTGA TGACATGCTC AATGTGTCTG GACATCTCCT GAGTACAGCA 
GAGGTGGAAT CGGCACTTGT GGAACACGAG GCTGTCGCAG AGGCAGCTGT GGTGGGCCAC 
CCTCATCCTG TGAAGGGCGA ATGCCTCTAC TGCTTTGTTA CCTTGTGTGA TGGCCATACC 
TTCAGCCCCA CACTCACAGA GGAACTCAAG AAGCAGATTA GAGAAAAGAT TGGCCCCATT 
GCCACACCAG ACTACATCCA GAATGCACCT GGCTTGCCTA AAACACGCTC AGGGAAAATC 
ATGAGGCGAG TTCTCCGGAA GATTGCTCAG AATGACCATG ACCTGGGGGA TACATCTACG 
GTGGCTGACC CATCTGTCAT CAACCATCTC TTCAGTCACC GCTGCCTGAC CACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acss2 ( XM_006499975.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500038.1 Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu30752
Clone ID Related Accession (Same CDS sequence)	XM_006499975.1
Accession Version	XM_006499975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	acetyl-coenzyme A synthetase, cytoplasmic isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTATGA TCTTGGAACT TGTGGTGGCT ATGCTGGCAT GCGCTCGCCT TGGAGCTTTG 
CACTCCATTG TGTTTGCAGG CTTCTCTGCA GAGTCTCTCT GTGAAAGGAT CTTGGATTCC 
AGTTGCTGCC TGCTCATCAC TACAGATGCC TTCTACAGGG GGGAAAAACT TGTGAACCTG 
AAGGAGCTGG CTGATGAGTC CTTGGAGAAG TGCCGAGAGA AGGGCTTCCC AGTGAGATGC 
TGCATTGTGG TCAAACATCT GGGGCGGGCA GAGCTGGGCA TGAATGACTC CCCCAGCCAG 
TCCCCACCAG TTAAGAGGCC ATGTCCAGAT GTCCAGGGTA AGCTGAGAGA GAAATCCAAG 
TGCATCCGAC CCCAGATCTG CTGGAACGAA GGGGTTGACT TATGGTGGCA TGAACTCATG 
CAGCAGGCAG GAGACGAGTG TGAGCCTGAG TGGTGTGATG CTGAGGACCC ACTCTTCATC 
TTGTACACCA GTGGCTCCAC AGGCAAACCT AAGGGTGTGG TGCACACAAT TGGAGGCTAT 
ATGCTCTATG TGGCTACAAC TTTCAAGTAT GTGTTTGATT TCCACCCGGA AGATGTGTTC 
TGGTGCACAG CAGACATCGG CTGGATCACT GGTCATTCCT ATGTCACCTA TGGGCCACTG 
GCTAATGGTG CCACTAGTGT TTTGTTTGAG GGGATCCCCA CATACCCAGA TGAAGGGCGC 
TTGTGGAGCA TTGTGGACAA ATACAAGGTG ACCAAGTTCT ACACGGCACC AACAGCCATC 
CGGATGCTCA TGAAGTTTGG AGATGATCCT GTCACCAAGC ATAGCCGGGC ATCCCTGCAG 
GTGCTGGGCA CAGTAGGTGA ACCCATCAAC CCTGAAGCCT GGCTATGGTA CCACCGGGTA 
GTAGGTTCCC AGCGCTGCCC CATTGTAGAC ACCTTCTGGC AAACAGAAAC AGGTGGCCAT 
ATGCTGACCC CTCTCCCTGG CGCCACACCC ATGAAACCTG GTTCTGCTTC TTTCCCATTC 
TTCGGTGTAG CGCCTGCAAT CCTGAATGAG TCCGGGGAGG AGCTGGAAGG GGAAGCTGAA 
GGTTATCTGG TGTTCAAGCA GCCCTGGCCA GGGATCATGC GCACAGTCTA TGGGAACCAC 
ACACGGTTTG AGACCACCTA CTTTAAGAAG TTCCCTGGCT ACTATGTGAC CGGAGATGGC 
TGCCGACGGG ATCAGGATGG CTATTACTGG ATCACGGGCA GGATTGATGA CATGCTCAAT 
GTGTCTGGAC ATCTCCTGAG TACAGCAGAG GTGGAATCGG CACTTGTGGA ACACGAGGCT 
GTCGCAGAGG CAGCTGTGGT GGGCCACCCT CATCCTGTGA AGGGCGAATG CCTCTACTGC 
TTTGTTACCT TGTGTGATGG CCATACCTTC AGCCCCACAC TCACAGAGGA ACTCAAGAAG 
CAGATTAGAG AAAAGATTGG CCCCATTGCC ACACCAGACT ACATCCAGAA TGCACCTGGC 
TTGCCTAAAA CACGCTCAGG GAAAATCATG AGGCGAGTTC TCCGGAAGAT TGCTCAGAAT 
GACCATGACC TGGGGGATAC ATCTACGGTG GCTGACCCAT CTGTCATCAA CCATCTCTTC 
AGTCACCGCT GCCTGACCAC CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500038.1
CDS	135..1781
Translation	MPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQGKLREKSKCIRPQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTTQ

Target ORF information:

RefSeq Version	XM_006499975.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499975.1

ORF Insert Sequence:

ATGCCTATGA TCTTGGAACT TGTGGTGGCT ATGCTGGCAT GCGCTCGCCT TGGAGCTTTG 
CACTCCATTG TGTTTGCAGG CTTCTCTGCA GAGTCTCTCT GTGAAAGGAT CTTGGATTCC 
AGTTGCTGCC TGCTCATCAC TACAGATGCC TTCTACAGGG GGGAAAAACT TGTGAACCTG 
AAGGAGCTGG CTGATGAGTC CTTGGAGAAG TGCCGAGAGA AGGGCTTCCC AGTGAGATGC 
TGCATTGTGG TCAAACATCT GGGGCGGGCA GAGCTGGGCA TGAATGACTC CCCCAGCCAG 
TCCCCACCAG TTAAGAGGCC ATGTCCAGAT GTCCAGGGTA AGCTGAGAGA GAAATCCAAG 
TGCATCCGAC CCCAGATCTG CTGGAACGAA GGGGTTGACT TATGGTGGCA TGAACTCATG 
CAGCAGGCAG GAGACGAGTG TGAGCCTGAG TGGTGTGATG CTGAGGACCC ACTCTTCATC 
TTGTACACCA GTGGCTCCAC AGGCAAACCT AAGGGTGTGG TGCACACAAT TGGAGGCTAT 
ATGCTCTATG TGGCTACAAC TTTCAAGTAT GTGTTTGATT TCCACCCGGA AGATGTGTTC 
TGGTGCACAG CAGACATCGG CTGGATCACT GGTCATTCCT ATGTCACCTA TGGGCCACTG 
GCTAATGGTG CCACTAGTGT TTTGTTTGAG GGGATCCCCA CATACCCAGA TGAAGGGCGC 
TTGTGGAGCA TTGTGGACAA ATACAAGGTG ACCAAGTTCT ACACGGCACC AACAGCCATC 
CGGATGCTCA TGAAGTTTGG AGATGATCCT GTCACCAAGC ATAGCCGGGC ATCCCTGCAG 
GTGCTGGGCA CAGTAGGTGA ACCCATCAAC CCTGAAGCCT GGCTATGGTA CCACCGGGTA 
GTAGGTTCCC AGCGCTGCCC CATTGTAGAC ACCTTCTGGC AAACAGAAAC AGGTGGCCAT 
ATGCTGACCC CTCTCCCTGG CGCCACACCC ATGAAACCTG GTTCTGCTTC TTTCCCATTC 
TTCGGTGTAG CGCCTGCAAT CCTGAATGAG TCCGGGGAGG AGCTGGAAGG GGAAGCTGAA 
GGTTATCTGG TGTTCAAGCA GCCCTGGCCA GGGATCATGC GCACAGTCTA TGGGAACCAC 
ACACGGTTTG AGACCACCTA CTTTAAGAAG TTCCCTGGCT ACTATGTGAC CGGAGATGGC 
TGCCGACGGG ATCAGGATGG CTATTACTGG ATCACGGGCA GGATTGATGA CATGCTCAAT 
GTGTCTGGAC ATCTCCTGAG TACAGCAGAG GTGGAATCGG CACTTGTGGA ACACGAGGCT 
GTCGCAGAGG CAGCTGTGGT GGGCCACCCT CATCCTGTGA AGGGCGAATG CCTCTACTGC 
TTTGTTACCT TGTGTGATGG CCATACCTTC AGCCCCACAC TCACAGAGGA ACTCAAGAAG 
CAGATTAGAG AAAAGATTGG CCCCATTGCC ACACCAGACT ACATCCAGAA TGCACCTGGC 
TTGCCTAAAA CACGCTCAGG GAAAATCATG AGGCGAGTTC TCCGGAAGAT TGCTCAGAAT 
GACCATGACC TGGGGGATAC ATCTACGGTG GCTGACCCAT CTGTCATCAA CCATCTCTTC 
AGTCACCGCT GCCTGACCAC CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acss2 ( XM_006499973.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500036.1 Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30750
Clone ID Related Accession (Same CDS sequence)	XM_006499973.1
Accession Version	XM_006499973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	acetyl-coenzyme A synthetase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAGAG CACGGCCTCT AGCCTCAGGC TCCGCCCTCA CCCGAGGCCC CGCCTCTAGG 
GTCGACCTGT TTTCCCCGCC CCGGCACCCG CAGCGACCGC AACGGCGGAC AAGGTGTTCG 
GAACTTGATG CGATGGGGCT TCCCGAGGAG CGGCGCAAGA GCGGCAGTGG AAGCCGGGCT 
CGTGAGGAGA CCGGCGCCGA GGGCCGGGTG CGGGGTTGGT CCCCGCCGCC GGAGGTCAGA 
CGCTCGGCGC ACGTCCCCTC TCTGCAGCGC TACCGCGAGC TGCACCGGCG TTCTGTGGAG 
GAGCCACGGG AGTTTTGGGG AAACATTGCC AAGGAATTTT ACTGGAAAAC CGCATGCCCT 
GGCCCATTCC TCCAGTACAA CTTTGATGTG ACTAAAGGGA AAATATTCAC TGAGTGGATG 
AAAGGAGCAA CTACAAACAT CTGCTACAAC GTGCTGGATC GAAATGTCCA TGAGAAAAAA 
CTTGGCGACA AAGTTGCTTT TTACTGGGAG GGCAATGAGC CAGGGGAGAC CACCAAGATC 
ACATACCGTG AACTCCTGGT CCAGGTGTGT CAGTTCAGCA ATGTTCTCCG TAAACAGGGC 
ATTCAGAAGG GTGACCGAGT GGCCATCTAC ATGCCTATGA TCTTGGAACT TGTGGTGGCT 
ATGCTGGCAT GCGCTCGCCT TGGAGCTTTG CACTCCATTG TGTTTGCAGG CTTCTCTGCA 
GAGTCTCTCT GTGAAAGGAT CTTGGATTCC AGTTGCTGCC TGCTCATCAC TACAGATGCC 
TTCTACAGGG GGGAAAAACT TGTGAACCTG AAGGAGCTGG CTGATGAGTC CTTGGAGAAG 
TGCCGAGAGA AGGGCTTCCC AGTGAGATGC TGCATTGTGG TCAAACATCT GGGGCGGGCA 
GAGCTGGGCA TGAATGACTC CCCCAGCCAG TCCCCACCAG TTAAGAGGCC ATGTCCAGAT 
GTCCAGGGTA AGCTGAGAGA GAAATCCAAG TGCATCCGAC CCCAGATCTG CTGGAACGAA 
GGGGTTGACT TATGGTGGCA TGAACTCATG CAGCAGGCAG GAGACGAGTG TGAGCCTGAG 
TGGTGTGATG CTGAGGACCC ACTCTTCATC TTGTACACCA GTGGCTCCAC AGGCAAACCT 
AAGGGTGTGG TGCACACAAT TGGAGGCTAT ATGCTCTATG TGGCTACAAC TTTCAAGTAT 
GTGTTTGATT TCCACCCGGA AGATGTGTTC TGGTGCACAG CAGACATCGG CTGGATCACT 
GGTCATTCCT ATGTCACCTA TGGGCCACTG GCTAATGGTG CCACTAGTGT TTTGTTTGAG 
GGGATCCCCA CATACCCAGA TGAAGGGCGC TTGTGGAGCA TTGTGGACAA ATACAAGGTG 
ACCAAGTTCT ACACGGCACC AACAGCCATC CGGATGCTCA TGAAGTTTGG AGATGATCCT 
GTCACCAAGC ATAGCCGGGC ATCCCTGCAG GTGCTGGGCA CAGTAGGTGA ACCCATCAAC 
CCTGAAGCCT GGCTATGGTA CCACCGGGTA GTAGGTTCCC AGCGCTGCCC CATTGTAGAC 
ACCTTCTGGC AAACAGAAAC AGGTGGCCAT ATGCTGACCC CTCTCCCTGG CGCCACACCC 
ATGAAACCTG GTTCTGCTTC TTTCCCATTC TTCGGTGTAG CGCCTGCAAT CCTGAATGAG 
TCCGGGGAGG AGCTGGAAGG GGAAGCTGAA GGTTATCTGG TGTTCAAGCA GCCCTGGCCA 
GGGATCATGC GCACAGTCTA TGGGAACCAC ACACGGTTTG AGACCACCTA CTTTAAGAAG 
TTCCCTGGCT ACTATGTGAC CGGAGATGGC TGCCGACGGG ATCAGGATGG CTATTACTGG 
ATCACGGGCA GGATTGATGA CATGCTCAAT GTGTCTGGAC ATCTCCTGAG TACAGCAGAG 
GTGGAATCGG CACTTGTGGA ACACGAGGCT GTCGCAGAGG CAGCTGTGGT GGGCCACCCT 
CATCCTGTGA AGGGCGAATG CCTCTACTGC TTTGTTACCT TGTGTGATGG CCATACCTTC 
AGCCCCACAC TCACAGAGGA ACTCAAGAAG CAGATTAGAG AAAAGATTGG CCCCATTGCC 
ACACCAGACT ACATCCAGAA TGCACCTGGC TTGCCTAAAA CACGCTCAGG GAAAATCATG 
AGGCGAGTTC TCCGGAAGAT TGCTCAGAAT GACCATGACC TGGGGGATAC ATCTACGGTG 
GCTGACCCAT CTGTCATCAA CCATCTCTTC AGTCACCGCT GCCTGACCAC CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500036.1
CDS	23..2299
Translation	MPRARPLASGSALTRGPASRVDLFSPPRHPQRPQRRTRCSELDAMGLPEERRKSGSGSRAREETGAEGRVRGWSPPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFYWKTACPGPFLQYNFDVTKGKIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQGKLREKSKCIRPQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTTQ

Target ORF information:

RefSeq Version	XM_006499973.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499973.1

ORF Insert Sequence:

ATGCCTAGAG CACGGCCTCT AGCCTCAGGC TCCGCCCTCA CCCGAGGCCC CGCCTCTAGG 
GTCGACCTGT TTTCCCCGCC CCGGCACCCG CAGCGACCGC AACGGCGGAC AAGGTGTTCG 
GAACTTGATG CGATGGGGCT TCCCGAGGAG CGGCGCAAGA GCGGCAGTGG AAGCCGGGCT 
CGTGAGGAGA CCGGCGCCGA GGGCCGGGTG CGGGGTTGGT CCCCGCCGCC GGAGGTCAGA 
CGCTCGGCGC ACGTCCCCTC TCTGCAGCGC TACCGCGAGC TGCACCGGCG TTCTGTGGAG 
GAGCCACGGG AGTTTTGGGG AAACATTGCC AAGGAATTTT ACTGGAAAAC CGCATGCCCT 
GGCCCATTCC TCCAGTACAA CTTTGATGTG ACTAAAGGGA AAATATTCAC TGAGTGGATG 
AAAGGAGCAA CTACAAACAT CTGCTACAAC GTGCTGGATC GAAATGTCCA TGAGAAAAAA 
CTTGGCGACA AAGTTGCTTT TTACTGGGAG GGCAATGAGC CAGGGGAGAC CACCAAGATC 
ACATACCGTG AACTCCTGGT CCAGGTGTGT CAGTTCAGCA ATGTTCTCCG TAAACAGGGC 
ATTCAGAAGG GTGACCGAGT GGCCATCTAC ATGCCTATGA TCTTGGAACT TGTGGTGGCT 
ATGCTGGCAT GCGCTCGCCT TGGAGCTTTG CACTCCATTG TGTTTGCAGG CTTCTCTGCA 
GAGTCTCTCT GTGAAAGGAT CTTGGATTCC AGTTGCTGCC TGCTCATCAC TACAGATGCC 
TTCTACAGGG GGGAAAAACT TGTGAACCTG AAGGAGCTGG CTGATGAGTC CTTGGAGAAG 
TGCCGAGAGA AGGGCTTCCC AGTGAGATGC TGCATTGTGG TCAAACATCT GGGGCGGGCA 
GAGCTGGGCA TGAATGACTC CCCCAGCCAG TCCCCACCAG TTAAGAGGCC ATGTCCAGAT 
GTCCAGGGTA AGCTGAGAGA GAAATCCAAG TGCATCCGAC CCCAGATCTG CTGGAACGAA 
GGGGTTGACT TATGGTGGCA TGAACTCATG CAGCAGGCAG GAGACGAGTG TGAGCCTGAG 
TGGTGTGATG CTGAGGACCC ACTCTTCATC TTGTACACCA GTGGCTCCAC AGGCAAACCT 
AAGGGTGTGG TGCACACAAT TGGAGGCTAT ATGCTCTATG TGGCTACAAC TTTCAAGTAT 
GTGTTTGATT TCCACCCGGA AGATGTGTTC TGGTGCACAG CAGACATCGG CTGGATCACT 
GGTCATTCCT ATGTCACCTA TGGGCCACTG GCTAATGGTG CCACTAGTGT TTTGTTTGAG 
GGGATCCCCA CATACCCAGA TGAAGGGCGC TTGTGGAGCA TTGTGGACAA ATACAAGGTG 
ACCAAGTTCT ACACGGCACC AACAGCCATC CGGATGCTCA TGAAGTTTGG AGATGATCCT 
GTCACCAAGC ATAGCCGGGC ATCCCTGCAG GTGCTGGGCA CAGTAGGTGA ACCCATCAAC 
CCTGAAGCCT GGCTATGGTA CCACCGGGTA GTAGGTTCCC AGCGCTGCCC CATTGTAGAC 
ACCTTCTGGC AAACAGAAAC AGGTGGCCAT ATGCTGACCC CTCTCCCTGG CGCCACACCC 
ATGAAACCTG GTTCTGCTTC TTTCCCATTC TTCGGTGTAG CGCCTGCAAT CCTGAATGAG 
TCCGGGGAGG AGCTGGAAGG GGAAGCTGAA GGTTATCTGG TGTTCAAGCA GCCCTGGCCA 
GGGATCATGC GCACAGTCTA TGGGAACCAC ACACGGTTTG AGACCACCTA CTTTAAGAAG 
TTCCCTGGCT ACTATGTGAC CGGAGATGGC TGCCGACGGG ATCAGGATGG CTATTACTGG 
ATCACGGGCA GGATTGATGA CATGCTCAAT GTGTCTGGAC ATCTCCTGAG TACAGCAGAG 
GTGGAATCGG CACTTGTGGA ACACGAGGCT GTCGCAGAGG CAGCTGTGGT GGGCCACCCT 
CATCCTGTGA AGGGCGAATG CCTCTACTGC TTTGTTACCT TGTGTGATGG CCATACCTTC 
AGCCCCACAC TCACAGAGGA ACTCAAGAAG CAGATTAGAG AAAAGATTGG CCCCATTGCC 
ACACCAGACT ACATCCAGAA TGCACCTGGC TTGCCTAAAA CACGCTCAGG GAAAATCATG 
AGGCGAGTTC TCCGGAAGAT TGCTCAGAAT GACCATGACC TGGGGGATAC ATCTACGGTG 
GCTGACCCAT CTGTCATCAA CCATCTCTTC AGTCACCGCT GCCTGACCAC CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acss2 ( NM_019811.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062785.2 Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), mRNA.

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CloneID	OMu11363
Clone ID Related Accession (Same CDS sequence)	NM_019811.3
Accession Version	NM_019811.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	acetyl-coenzyme A synthetase, cytoplasmic
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844852.11. On Mar 21, 2007 this sequence version replaced NM_019811.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051432.1, AK093831.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTTC CCGAGGAGCG GCGCAAGAGC GGCAGTGGAA GCCGGGCTCG TGAGGAGACC 
GGCGCCGAGG GCCGGGTGCG GGGTTGGTCC CCGCCGCCGG AGGTCAGACG CTCGGCGCAC 
GTCCCCTCTC TGCAGCGCTA CCGCGAGCTG CACCGGCGTT CTGTGGAGGA GCCACGGGAG 
TTTTGGGGAA ACATTGCCAA GGAATTTTAC TGGAAAACCG CATGCCCTGG CCCATTCCTC 
CAGTACAACT TTGATGTGAC TAAAGGGAAA ATATTCACTG AGTGGATGAA AGGAGCAACT 
ACAAACATCT GCTACAACGT GCTGGATCGA AATGTCCATG AGAAAAAACT TGGCGACAAA 
GTTGCTTTTT ACTGGGAGGG CAATGAGCCA GGGGAGACCA CCAAGATCAC ATACCGTGAA 
CTCCTGGTCC AGGTGTGTCA GTTCAGCAAT GTTCTCCGTA AACAGGGCAT TCAGAAGGGT 
GACCGAGTGG CCATCTACAT GCCTATGATC TTGGAACTTG TGGTGGCTAT GCTGGCATGC 
GCTCGCCTTG GAGCTTTGCA CTCCATTGTG TTTGCAGGCT TCTCTGCAGA GTCTCTCTGT 
GAAAGGATCT TGGATTCCAG TTGCTGCCTG CTCATCACTA CAGATGCCTT CTACAGGGGG 
GAAAAACTTG TGAACCTGAA GGAGCTGGCT GATGAGTCCT TGGAGAAGTG CCGAGAGAAG 
GGCTTCCCAG TGAGATGCTG CATTGTGGTC AAACATCTGG GGCGGGCAGA GCTGGGCATG 
AATGACTCCC CCAGCCAGTC CCCACCAGTT AAGAGGCCAT GTCCAGATGT CCAGATCTGC 
TGGAACGAAG GGGTTGACTT ATGGTGGCAT GAACTCATGC AGCAGGCAGG AGACGAGTGT 
GAGCCTGAGT GGTGTGATGC TGAGGACCCA CTCTTCATCT TGTACACCAG TGGCTCCACA 
GGCAAACCTA AGGGTGTGGT GCACACAATT GGAGGCTATA TGCTCTATGT GGCTACAACT 
TTCAAGTATG TGTTTGATTT CCACCCGGAA GATGTGTTCT GGTGCACAGC AGACATCGGC 
TGGATCACTG GTCATTCCTA TGTCACCTAT GGGCCACTGG CTAATGGTGC CACTAGTGTT 
TTGTTTGAGG GGATCCCCAC ATACCCAGAT GAAGGGCGCT TGTGGAGCAT TGTGGACAAA 
TACAAGGTGA CCAAGTTCTA CACGGCACCA ACAGCCATCC GGATGCTCAT GAAGTTTGGA 
GATGATCCTG TCACCAAGCA TAGCCGGGCA TCCCTGCAGG TGCTGGGCAC AGTAGGTGAA 
CCCATCAACC CTGAAGCCTG GCTATGGTAC CACCGGGTAG TAGGTTCCCA GCGCTGCCCC 
ATTGTAGACA CCTTCTGGCA AACAGAAACA GGTGGCCATA TGCTGACCCC TCTCCCTGGC 
GCCACACCCA TGAAACCTGG TTCTGCTTCT TTCCCATTCT TCGGTGTAGC GCCTGCAATC 
CTGAATGAGT CCGGGGAGGA GCTGGAAGGG GAAGCTGAAG GTTATCTGGT GTTCAAGCAG 
CCCTGGCCAG GGATCATGCG CACAGTCTAT GGGAACCACA CACGGTTTGA GACCACCTAC 
TTTAAGAAGT TCCCTGGCTA CTATGTGACC GGAGATGGCT GCCGACGGGA TCAGGATGGC 
TATTACTGGA TCACGGGCAG GATTGATGAC ATGCTCAATG TGTCTGGACA TCTCCTGAGT 
ACAGCAGAGG TGGAATCGGC ACTTGTGGAA CACGAGGCTG TCGCAGAGGC AGCTGTGGTG 
GGCCACCCTC ATCCTGTGAA GGGCGAATGC CTCTACTGCT TTGTTACCTT GTGTGATGGC 
CATACCTTCA GCCCCACACT CACAGAGGAA CTCAAGAAGC AGATTAGAGA AAAGATTGGC 
CCCATTGCCA CACCAGACTA CATCCAGAAT GCACCTGGCT TGCCTAAAAC ACGCTCAGGG 
AAAATCATGA GGCGAGTTCT CCGGAAGATT GCTCAGAATG ACCATGACCT GGGGGATACA 
TCTACGGTGG CTGACCCATC TGTCATCAAC CATCTCTTCA GTCACCGCTG CCTGACCACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062785.2
CDS	65..2170
Translation	MGLPEERRKSGSGSRAREETGAEGRVRGWSPPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFYWKTACPGPFLQYNFDVTKGKIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTTQ

Target ORF information:

RefSeq Version	NM_019811.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019811.3

ORF Insert Sequence:

ATGGGGCTTC CCGAGGAGCG GCGCAAGAGC GGCAGTGGAA GCCGGGCTCG TGAGGAGACC 
GGCGCCGAGG GCCGGGTGCG GGGTTGGTCC CCGCCGCCGG AGGTCAGACG CTCGGCGCAC 
GTCCCCTCTC TGCAGCGCTA CCGCGAGCTG CACCGGCGTT CTGTGGAGGA GCCACGGGAG 
TTTTGGGGAA ACATTGCCAA GGAATTTTAC TGGAAAACCG CATGCCCTGG CCCATTCCTC 
CAGTACAACT TTGATGTGAC TAAAGGGAAA ATATTCACTG AGTGGATGAA AGGAGCAACT 
ACAAACATCT GCTACAACGT GCTGGATCGA AATGTCCATG AGAAAAAACT TGGCGACAAA 
GTTGCTTTTT ACTGGGAGGG CAATGAGCCA GGGGAGACCA CCAAGATCAC ATACCGTGAA 
CTCCTGGTCC AGGTGTGTCA GTTCAGCAAT GTTCTCCGTA AACAGGGCAT TCAGAAGGGT 
GACCGAGTGG CCATCTACAT GCCTATGATC TTGGAACTTG TGGTGGCTAT GCTGGCATGC 
GCTCGCCTTG GAGCTTTGCA CTCCATTGTG TTTGCAGGCT TCTCTGCAGA GTCTCTCTGT 
GAAAGGATCT TGGATTCCAG TTGCTGCCTG CTCATCACTA CAGATGCCTT CTACAGGGGG 
GAAAAACTTG TGAACCTGAA GGAGCTGGCT GATGAGTCCT TGGAGAAGTG CCGAGAGAAG 
GGCTTCCCAG TGAGATGCTG CATTGTGGTC AAACATCTGG GGCGGGCAGA GCTGGGCATG 
AATGACTCCC CCAGCCAGTC CCCACCAGTT AAGAGGCCAT GTCCAGATGT CCAGATCTGC 
TGGAACGAAG GGGTTGACTT ATGGTGGCAT GAACTCATGC AGCAGGCAGG AGACGAGTGT 
GAGCCTGAGT GGTGTGATGC TGAGGACCCA CTCTTCATCT TGTACACCAG TGGCTCCACA 
GGCAAACCTA AGGGTGTGGT GCACACAATT GGAGGCTATA TGCTCTATGT GGCTACAACT 
TTCAAGTATG TGTTTGATTT CCACCCGGAA GATGTGTTCT GGTGCACAGC AGACATCGGC 
TGGATCACTG GTCATTCCTA TGTCACCTAT GGGCCACTGG CTAATGGTGC CACTAGTGTT 
TTGTTTGAGG GGATCCCCAC ATACCCAGAT GAAGGGCGCT TGTGGAGCAT TGTGGACAAA 
TACAAGGTGA CCAAGTTCTA CACGGCACCA ACAGCCATCC GGATGCTCAT GAAGTTTGGA 
GATGATCCTG TCACCAAGCA TAGCCGGGCA TCCCTGCAGG TGCTGGGCAC AGTAGGTGAA 
CCCATCAACC CTGAAGCCTG GCTATGGTAC CACCGGGTAG TAGGTTCCCA GCGCTGCCCC 
ATTGTAGACA CCTTCTGGCA AACAGAAACA GGTGGCCATA TGCTGACCCC TCTCCCTGGC 
GCCACACCCA TGAAACCTGG TTCTGCTTCT TTCCCATTCT TCGGTGTAGC GCCTGCAATC 
CTGAATGAGT CCGGGGAGGA GCTGGAAGGG GAAGCTGAAG GTTATCTGGT GTTCAAGCAG 
CCCTGGCCAG GGATCATGCG CACAGTCTAT GGGAACCACA CACGGTTTGA GACCACCTAC 
TTTAAGAAGT TCCCTGGCTA CTATGTGACC GGAGATGGCT GCCGACGGGA TCAGGATGGC 
TATTACTGGA TCACGGGCAG GATTGATGAC ATGCTCAATG TGTCTGGACA TCTCCTGAGT 
ACAGCAGAGG TGGAATCGGC ACTTGTGGAA CACGAGGCTG TCGCAGAGGC AGCTGTGGTG 
GGCCACCCTC ATCCTGTGAA GGGCGAATGC CTCTACTGCT TTGTTACCTT GTGTGATGGC 
CATACCTTCA GCCCCACACT CACAGAGGAA CTCAAGAAGC AGATTAGAGA AAAGATTGGC 
CCCATTGCCA CACCAGACTA CATCCAGAAT GCACCTGGCT TGCCTAAAAC ACGCTCAGGG 
AAAATCATGA GGCGAGTTCT CCGGAAGATT GCTCAGAATG ACCATGACCT GGGGGATACA 
TCTACGGTGG CTGACCCATC TGTCATCAAC CATCTCTTCA GTCACCGCTG CCTGACCACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acss2 ( XM_017319156.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174645.1 Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X2, mRNA.

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CloneID	OMu80355
Clone ID Related Accession (Same CDS sequence)	XM_017319156.1
Accession Version	XM_017319156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	acetyl-coenzyme A synthetase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAGAG CACGGCCTCT AGCCTCAGGC TCCGCCCTCA CCCGAGGCCC CGCCTCTAGG 
GTCGACCTGT TTTCCCCGCC CCGGCACCCG CAGCGACCGC AACGGCGGAC AAGGTGTTCG 
GAACTTGATG CGATGGGGCT TCCCGAGGAG CGGCGCAAGA GCGGCAGTGG AAGCCGGGCT 
CGTGAGGAGA CCGGCGCCGA GGGCCGGGTG CGGGGTTGGT CCCCGCCGCC GGAGGTCAGA 
CGCTCGGCGC ACGTCCCCTC TCTGCAGCGC TACCGCGAGC TGCACCGGCG TTCTGTGGAG 
GAGCCACGGG AGTTTTGGGG AAACATTGCC AAGGAATTTT ACTGGAAAAC CGCATGCCCT 
GGCCCATTCC TCCAGTACAA CTTTGATGTG ACTAAAGGGA AAATATTCAC TGAGTGGATG 
AAAGGAGCAA CTACAAACAT CTGCTACAAC GTGCTGGATC GAAATGTCCA TGAGAAAAAA 
CTTGGCGACA AAGTTGCTTT TTACTGGGAG GGCAATGAGC CAGGGGAGAC CACCAAGATC 
ACATACCGTG AACTCCTGGT CCAGGTGTGT CAGTTCAGCA ATGTTCTCCG TAAACAGGGC 
ATTCAGAAGG GTGACCGAGT GGCCATCTAC ATGCCTATGA TCTTGGAACT TGTGGTGGCT 
ATGCTGGCAT GCGCTCGCCT TGGAGCTTTG CACTCCATTG TGTTTGCAGG CTTCTCTGCA 
GAGTCTCTCT GTGAAAGGAT CTTGGATTCC AGTTGCTGCC TGCTCATCAC TACAGATGCC 
TTCTACAGGG GGGAAAAACT TGTGAACCTG AAGGAGCTGG CTGATGAGTC CTTGGAGAAG 
TGCCGAGAGA AGGGCTTCCC AGTGAGATGC TGCATTGTGG TCAAACATCT GGGGCGGGCA 
GAGCTGGGCA TGAATGACTC CCCCAGCCAG TCCCCACCAG TTAAGAGGCC ATGTCCAGAT 
GTCCAGGGTA AGCTGAGAGA GAAATCCAAG TGCATCCGAC CCCAGATCTG CTGGAACGAA 
GGGGTTGACT TATGGTGGCA TGAACTCATG CAGCAGGCAG GAGACGAGTG TGAGCCTGAG 
TGGTGTGATG CTGAGGACCC ACTCTTCATC TTGTACACCA GTGGCTCCAC AGGCAAACCT 
AAGTTTGAGG GGATCCCCAC ATACCCAGAT GAAGGGCGCT TGTGGAGCAT TGTGGACAAA 
TACAAGGTGA CCAAGTTCTA CACGGCACCA ACAGCCATCC GGATGCTCAT GAAGTTTGGA 
GATGATCCTG TCACCAAGCA TAGCCGGGCA TCCCTGCAGG TGCTGGGCAC AGTAGGTGAA 
CCCATCAACC CTGAAGCCTG GCTATGGTAC CACCGGGTAG TAGGTTCCCA GCGCTGCCCC 
ATTGTAGACA CCTTCTGGCA AACAGAAACA GGTGGCCATA TGCTGACCCC TCTCCCTGGC 
GCCACACCCA TGAAACCTGG TTCTGCTTCT TTCCCATTCT TCGGTGTAGC GCCTGCAATC 
CTGAATGAGT CCGGGGAGGA GCTGGAAGGG GAAGCTGAAG GTTATCTGGT GTTCAAGCAG 
CCCTGGCCAG GGATCATGCG CACAGTCTAT GGGAACCACA CACGGTTTGA GACCACCTAC 
TTTAAGAAGT TCCCTGGCTA CTATGTGACC GGAGATGGCT GCCGACGGGA TCAGGATGGC 
TATTACTGGA TCACGGGCAG GATTGATGAC ATGCTCAATG TGTCTGGACA TCTCCTGAGT 
ACAGCAGAGG TGGAATCGGC ACTTGTGGAA CACGAGGCTG TCGCAGAGGC AGCTGTGGTG 
GGCCACCCTC ATCCTGTGAA GGGCGAATGC CTCTACTGCT TTGTTACCTT GTGTGATGGC 
CATACCTTCA GCCCCACACT CACAGAGGAA CTCAAGAAGC AGATTAGAGA AAAGATTGGC 
CCCATTGCCA CACCAGACTA CATCCAGAAT GCACCTGGCT TGCCTAAAAC ACGCTCAGGG 
AAAATCATGA GGCGAGTTCT CCGGAAGATT GCTCAGAATG ACCATGACCT GGGGGATACA 
TCTACGGTGG CTGACCCATC TGTCATCAAC CATCTCTTCA GTCACCGCTG CCTGACCACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174645.1
CDS	23..2128
Translation	MPRARPLASGSALTRGPASRVDLFSPPRHPQRPQRRTRCSELDAMGLPEERRKSGSGSRAREETGAEGRVRGWSPPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFYWKTACPGPFLQYNFDVTKGKIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQGKLREKSKCIRPQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTTQ

Target ORF information:

RefSeq Version	XM_017319156.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319156.1

ORF Insert Sequence:

ATGCCTAGAG CACGGCCTCT AGCCTCAGGC TCCGCCCTCA CCCGAGGCCC CGCCTCTAGG 
GTCGACCTGT TTTCCCCGCC CCGGCACCCG CAGCGACCGC AACGGCGGAC AAGGTGTTCG 
GAACTTGATG CGATGGGGCT TCCCGAGGAG CGGCGCAAGA GCGGCAGTGG AAGCCGGGCT 
CGTGAGGAGA CCGGCGCCGA GGGCCGGGTG CGGGGTTGGT CCCCGCCGCC GGAGGTCAGA 
CGCTCGGCGC ACGTCCCCTC TCTGCAGCGC TACCGCGAGC TGCACCGGCG TTCTGTGGAG 
GAGCCACGGG AGTTTTGGGG AAACATTGCC AAGGAATTTT ACTGGAAAAC CGCATGCCCT 
GGCCCATTCC TCCAGTACAA CTTTGATGTG ACTAAAGGGA AAATATTCAC TGAGTGGATG 
AAAGGAGCAA CTACAAACAT CTGCTACAAC GTGCTGGATC GAAATGTCCA TGAGAAAAAA 
CTTGGCGACA AAGTTGCTTT TTACTGGGAG GGCAATGAGC CAGGGGAGAC CACCAAGATC 
ACATACCGTG AACTCCTGGT CCAGGTGTGT CAGTTCAGCA ATGTTCTCCG TAAACAGGGC 
ATTCAGAAGG GTGACCGAGT GGCCATCTAC ATGCCTATGA TCTTGGAACT TGTGGTGGCT 
ATGCTGGCAT GCGCTCGCCT TGGAGCTTTG CACTCCATTG TGTTTGCAGG CTTCTCTGCA 
GAGTCTCTCT GTGAAAGGAT CTTGGATTCC AGTTGCTGCC TGCTCATCAC TACAGATGCC 
TTCTACAGGG GGGAAAAACT TGTGAACCTG AAGGAGCTGG CTGATGAGTC CTTGGAGAAG 
TGCCGAGAGA AGGGCTTCCC AGTGAGATGC TGCATTGTGG TCAAACATCT GGGGCGGGCA 
GAGCTGGGCA TGAATGACTC CCCCAGCCAG TCCCCACCAG TTAAGAGGCC ATGTCCAGAT 
GTCCAGGGTA AGCTGAGAGA GAAATCCAAG TGCATCCGAC CCCAGATCTG CTGGAACGAA 
GGGGTTGACT TATGGTGGCA TGAACTCATG CAGCAGGCAG GAGACGAGTG TGAGCCTGAG 
TGGTGTGATG CTGAGGACCC ACTCTTCATC TTGTACACCA GTGGCTCCAC AGGCAAACCT 
AAGTTTGAGG GGATCCCCAC ATACCCAGAT GAAGGGCGCT TGTGGAGCAT TGTGGACAAA 
TACAAGGTGA CCAAGTTCTA CACGGCACCA ACAGCCATCC GGATGCTCAT GAAGTTTGGA 
GATGATCCTG TCACCAAGCA TAGCCGGGCA TCCCTGCAGG TGCTGGGCAC AGTAGGTGAA 
CCCATCAACC CTGAAGCCTG GCTATGGTAC CACCGGGTAG TAGGTTCCCA GCGCTGCCCC 
ATTGTAGACA CCTTCTGGCA AACAGAAACA GGTGGCCATA TGCTGACCCC TCTCCCTGGC 
GCCACACCCA TGAAACCTGG TTCTGCTTCT TTCCCATTCT TCGGTGTAGC GCCTGCAATC 
CTGAATGAGT CCGGGGAGGA GCTGGAAGGG GAAGCTGAAG GTTATCTGGT GTTCAAGCAG 
CCCTGGCCAG GGATCATGCG CACAGTCTAT GGGAACCACA CACGGTTTGA GACCACCTAC 
TTTAAGAAGT TCCCTGGCTA CTATGTGACC GGAGATGGCT GCCGACGGGA TCAGGATGGC 
TATTACTGGA TCACGGGCAG GATTGATGAC ATGCTCAATG TGTCTGGACA TCTCCTGAGT 
ACAGCAGAGG TGGAATCGGC ACTTGTGGAA CACGAGGCTG TCGCAGAGGC AGCTGTGGTG 
GGCCACCCTC ATCCTGTGAA GGGCGAATGC CTCTACTGCT TTGTTACCTT GTGTGATGGC 
CATACCTTCA GCCCCACACT CACAGAGGAA CTCAAGAAGC AGATTAGAGA AAAGATTGGC 
CCCATTGCCA CACCAGACTA CATCCAGAAT GCACCTGGCT TGCCTAAAAC ACGCTCAGGG 
AAAATCATGA GGCGAGTTCT CCGGAAGATT GCTCAGAATG ACCATGACCT GGGGGATACA 
TCTACGGTGG CTGACCCATC TGTCATCAAC CATCTCTTCA GTCACCGCTG CCTGACCACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.
Nature546(7658)381-386(2017 06)
Mews P,Donahue G,Drake AM,Luczak V,Abel T,Berger SL

Possible involvement of ACSS2 gene in alcoholism.
Journal of neural transmission (Vienna, Austria : 1996)(2017 May)
Ribeiro AF,de Lacerda RB,Correia D,Brunialti-Godard AL,de Miranda DM,Campos VR,de Souza VF,Ribeiro AM

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.
Proceedings of the National Academy of Sciences of the United States of America113(26)7219-24(2016 06)
Chen L,Chen Q,Xie B,Quan C,Sheng Y,Zhu S,Rong P,Zhou S,Sakamoto K,MacKintosh C,Wang HY,Chen S

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Acss2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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