Cldn15 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cldn15
Entrez Gene ID	60363
Full Name	claudin 15
Synonyms	2210009B08Rik,BB107105
General protein information	Preferred Names claudin 15 Names claudin-15
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2
Summary	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein increases permeability for sodium ions in anion-selective epithelial cell sheets. The gene deficiency leads to megaintestine and decreases in intestinal epithelial paracellular ion permeability. This gene is a direct target for hepatocyte-nuclear-factor-4alpha, a mediator of ion epithelial transport, and is down-modulated in inflammatory bowel disease. [provided by RefSeq, Aug 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021719.4	NP_068365.1	claudin-15

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLDN15	XP_519278.3
Danio rerio (zebrafish)	cldn15b	NP_001035404.1
Xenopus tropicalis (tropical clawed frog)	cldn15.1	NP_001096379.1
Rattus norvegicus (Norway rat)	Cldn15	NP_001100605.1
Danio rerio (zebrafish)	cldn15a	NP_956698.1
Mus musculus (house mouse)	Cldn15	NP_068365.1
Homo sapiens (human)	CLDN15	NP_001172009.1
Bos taurus (cattle)	CLDN15	NP_001039651.1

Gene Ontology
Bibliography

Related articles in PubMed

Delayed Hair Follicle Morphogenesis and Hair Follicle Dystrophy in a Lipoatrophy Mouse Model of Pparg Total Deletion.
Sardella C, Winkler C, Quignodon L, Hardman JA, Toffoli B, Giordano Attianese GMP, Hundt JE, Michalik L, Vinson CR, Paus R, Desvergne B, Gilardi F
The Journal of investigative dermatology(2017 Sep)

Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice.
Hu X, Tang Z, Li Y, Liu W, Zhang S, Wang B, Tian Y, Zhao Y, Ran H, Liu W, Feng GS, Shuai J, Wang H, Lu Z
Scientific reports512982(2015 Aug)

Crystal structure of a claudin provides insight into the architecture of tight junctions.
Suzuki H, Nishizawa T, Tani K, Yamazaki Y, Tamura A, Ishitani R, Dohmae N, Tsukita S, Nureki O, Fujiyoshi Y
Science (New York, N.Y.)344(6181)304-7(2014 Apr)

EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.
Lei Z, Maeda T, Tamura A, Nakamura T, Yamazaki Y, Shiratori H, Yashiro K, Tsukita S, Hamada H
Developmental biology371(2)136-45(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

structure of claudin-15 at a resolution of 2.4 angstroms; structure reveals a characteristic beta-sheet fold comprising 2 extracellular segments, which is anchored to a transmembrane four-helix bundle by a consensus motif; findings provide insight into molecular basis of ion homeostasis across tight junctions
Title: Crystal structure of a claudin provides insight into the architecture of tight junctions.

Claudin-15 knockout leads to Na(+) deficiency and glucose malabsorption in the mouse adult small intestine.
Title: Loss of claudin-15, but not claudin-2, causes Na+ deficiency and glucose malabsorption in mouse small intestine.

Claudin-15-based formation of tight junctions to organize the microenvironment including ion conductance is important for normal-sized morphogenesis of the small intestine.
Title: Megaintestine in claudin-15-deficient mice.

The following Cldn15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cldn15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19462	NM_021719.4 Latest version!	Mus musculus claudin 15 (Cldn15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cldn15 ( NM_021719.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_068365.1 Mus musculus claudin 15 (Cldn15), mRNA.

Price & Availability↑

CloneID	OMu19462
Clone ID Related Accession (Same CDS sequence)	NM_021719.4
Accession Version	NM_021719.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	claudin-15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK008683.1, BB801129.1 and CF545718.1. On Aug 11, 2010 this sequence version replaced NM_021719.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC023428.1, AK008683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGTAG CTGTGGAGAC CTTCGGCTTC TTCATGTCAG CCCTGGGACT GCTGATGCTG 
GGGTTGACCC TTTCAAACAG CTACTGGAGA GTGTCTACGG TCCATGGCAA CGTCATCACC 
ACCAACACCA TCTTTGAGAA CCTGTGGTAC AGCTGTGCCA CCGACTCCCT GGGAGTCTCC 
AACTGCTGGG ACTTTCCGTC CATGCTGGCC CTCTCTGGGT ATGTCCAGGG CTGCCGGGCT 
CTCATGATCA CCGCCATCCT CCTGGGCTTC CTGGGCCTCT TTCTAGGCAT GGTGGGACTC 
CGCTGCACCA ACGTGGGCAA CATGGATCTC TCCAAGAAGG CCAAGCTGCT GGCCATTGCA 
GGGACCCTCC ACATACTTGC TGGAGCCTGT GGGATGGTGG CTATCTCGTG GTACGCCGTC 
AACATCACTA CTGACTTCTT CAACCCACTG TATGCTGGAA CCAAGTATGA ACTGGGCCCC 
GCCCTCTACT TGGGCTGGAG TGCCTCCCTG CTCTCCATCC TGGGCGGCAT CTGTGTCTTC 
TCCACCTGCT GCTGTTCCTC CAAGGAGGAA CCAGCCACCA GGGCTGGGCT TCCCTACAAG 
CCTTCTACGG TTGTGATACC CCGTGCCACC TCGGATGAGA GTGACATCAG CTTCGGTAAA 
TATGGCAAAA ACGCATACGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068365.1
CDS	239..922
Translation	MSVAVETFGFFMSALGLLMLGLTLSNSYWRVSTVHGNVITTNTIFENLWYSCATDSLGVSNCWDFPSMLALSGYVQGCRALMITAILLGFLGLFLGMVGLRCTNVGNMDLSKKAKLLAIAGTLHILAGACGMVAISWYAVNITTDFFNPLYAGTKYELGPALYLGWSASLLSILGGICVFSTCCCSSKEEPATRAGLPYKPSTVVIPRATSDESDISFGKYGKNAYV

Target ORF information:

RefSeq Version	NM_021719.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 15 (Cldn15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021719.4

ORF Insert Sequence:

ATGTCGGTAG CTGTGGAGAC CTTCGGCTTC TTCATGTCAG CCCTGGGACT GCTGATGCTG 
GGGTTGACCC TTTCAAACAG CTACTGGAGA GTGTCTACGG TCCATGGCAA CGTCATCACC 
ACCAACACCA TCTTTGAGAA CCTGTGGTAC AGCTGTGCCA CCGACTCCCT GGGAGTCTCC 
AACTGCTGGG ACTTTCCGTC CATGCTGGCC CTCTCTGGGT ATGTCCAGGG CTGCCGGGCT 
CTCATGATCA CCGCCATCCT CCTGGGCTTC CTGGGCCTCT TTCTAGGCAT GGTGGGACTC 
CGCTGCACCA ACGTGGGCAA CATGGATCTC TCCAAGAAGG CCAAGCTGCT GGCCATTGCA 
GGGACCCTCC ACATACTTGC TGGAGCCTGT GGGATGGTGG CTATCTCGTG GTACGCCGTC 
AACATCACTA CTGACTTCTT CAACCCACTG TATGCTGGAA CCAAGTATGA ACTGGGCCCC 
GCCCTCTACT TGGGCTGGAG TGCCTCCCTG CTCTCCATCC TGGGCGGCAT CTGTGTCTTC 
TCCACCTGCT GCTGTTCCTC CAAGGAGGAA CCAGCCACCA GGGCTGGGCT TCCCTACAAG 
CCTTCTACGG TTGTGATACC CCGTGCCACC TCGGATGAGA GTGACATCAG CTTCGGTAAA 
TATGGCAAAA ACGCATACGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Delayed Hair Follicle Morphogenesis and Hair Follicle Dystrophy in a Lipoatrophy Mouse Model of Pparg Total Deletion.
The Journal of investigative dermatology(2017 Sep)
Sardella C,Winkler C,Quignodon L,Hardman JA,Toffoli B,Giordano Attianese GMP,Hundt JE,Michalik L,Vinson CR,Paus R,Desvergne B,Gilardi F

Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice.
Scientific reports512982(2015 Aug)
Hu X,Tang Z,Li Y,Liu W,Zhang S,Wang B,Tian Y,Zhao Y,Ran H,Liu W,Feng GS,Shuai J,Wang H,Lu Z

Crystal structure of a claudin provides insight into the architecture of tight junctions.
Science (New York, N.Y.)344(6181)304-7(2014 Apr)
Suzuki H,Nishizawa T,Tani K,Yamazaki Y,Tamura A,Ishitani R,Dohmae N,Tsukita S,Nureki O,Fujiyoshi Y

EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.
Developmental biology371(2)136-45(2012 Nov)
Lei Z,Maeda T,Tamura A,Nakamura T,Yamazaki Y,Shiratori H,Yashiro K,Tsukita S,Hamada H

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Cldn15 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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