Evc cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Evc
Entrez Gene ID	59056
Full Name	EvC ciliary complex subunit 1
General protein information	Preferred Names EvC ciliary complex subunit 1 Names ellis-van Creveld syndrome protein homolog Ellis van Creveld gene homolog Ellis van Creveld gene syndrome EvC protein (Ellis-van Creveld syndrome)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321049.1	XP_017176538.1	ellis-van Creveld syndrome protein homolog isoform X4
NM_021292.2	NP_067267.2	ellis-van Creveld syndrome protein homolog
XM_006504042.3	XP_006504105.1	ellis-van Creveld syndrome protein homolog isoform X1
XM_006504043.3	XP_006504106.1	ellis-van Creveld syndrome protein homolog isoform X3
XM_006504042.4	XP_006504105.1	ellis-van Creveld syndrome protein homolog isoform X1
XM_011240761.3	XP_011239063.1	ellis-van Creveld syndrome protein homolog isoform X2
XM_011240761.2	XP_011239063.1	ellis-van Creveld syndrome protein homolog isoform X2
XM_006504043.4	XP_006504106.1	ellis-van Creveld syndrome protein homolog isoform X3

Pathways from BioSystems

Homologies

	EVC	XP_001093041.2
Mus musculus (house mouse)	Evc	NP_067267.2
Rattus norvegicus (Norway rat)	Evc	NP_001163910.1
Homo sapiens (human)	EVC	NP_714928.1
Canis lupus familiaris (dog)	EVC	XP_853902.2
Gallus gallus (chicken)	EVC	NP_001005347.1
Xenopus tropicalis (tropical clawed frog)	evc	NP_001096234.1
Pan troglodytes (chimpanzee)	EVC	XP_517090.3
Bos taurus (cattle)	EVC	NP_777172.1

Gene Ontology
Bibliography

Related articles in PubMed

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Burnicka-Turek O, Steimle JD, Huang W, Felker L, Kamp A, Kweon J, Peterson M, Reeves RH, Maslen CL, Gruber PJ, Yang XH, Shendure J, Moskowitz IP
Human molecular genetics25(14)3011-3028(2016 07)

EFCAB7 and IQCE regulate hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia.
Pusapati GV, Hughes CE, Dorn KV, Zhang D, Sugianto P, Aravind L, Rohatgi R
Developmental cell28(5)483-96(2014 Mar)

Evc regulates a symmetrical response to Shh signaling in molar development.
Nakatomi M, Hovorakova M, Gritli-Linde A, Blair HJ, MacArthur K, Peterka M, Lesot H, Peterkova R, Ruiz-Perez VL, Goodship JA, Peters H
Journal of dental research92(3)222-8(2013 Mar)

The ciliary Evc/Evc2 complex interacts with Smo and controls Hedgehog pathway activity in chondrocytes by regulating Sufu/Gli3 dissociation and Gli3 trafficking in primary cilia.
Caparr?s-Mart?n JA, Valencia M, Reytor E, Pacheco M, Fernandez M, Perez-Aytes A, Gean E, Lapunzina P, Peters H, Goodship JA, Ruiz-Perez VL
Human molecular genetics22(1)124-39(2013 Jan)

Smoothened transduces Hedgehog signal by forming a complex with Evc/Evc2.
Yang C, Chen W, Chen Y, Jiang J
Cell research22(11)1593-604(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

it is proposed that Hedgehog activates Smo by inducing its phosphorylation, which recruits Evc/Evc2 to activate Gli proteins by antagonizing Sufu in the primary cilia
Title: Smoothened transduces Hedgehog signal by forming a complex with Evc/Evc2.

Data show that Evc promotes chondrocyte proliferation, chondrocyte hypertrophy and the differentiation of osteoblasts in the perichondrium and reveal that Evc is essential for intrasphenoidal synchondrosis development.
Title: Evc works in chondrocytes and osteoblasts to regulate multiple aspects of growth plate development in the appendicular skeleton and cranial base.

the presence of Evc and Evc2 at the basal body and cilia membrane is co-dependent. In addition, Evc2, but not Evc, is present in the cell nucleus suggesting movement of Evc2 between the cilium and nucleus.
Title: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus.

The expression of a Weyer variant, but not the expression of a truncated protein that mimics an Ellis-van Creveld syndrome mutation, impairs Hedgehog signal transduction in NIH 3T3 cells in keeping with its dominant effect.
Title: Widening the mutation spectrum of EVC and EVC2: ectopic expression of Weyer variants in NIH 3T3 fibroblasts disrupts Hedgehog signaling.

EVC and LBN protein colocalize at the tip of the primary atrial septum during murine cardiac development.
Title: Analysis of Ellis van Creveld syndrome gene products: implications for cardiovascular development and disease.

The following Evc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Evc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu35151	XM_006504042.4 Latest version!	Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu35152	XM_006504043.4 Latest version!	Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu69824	XM_011240761.3 Latest version!	Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu81459	XM_017321049.1 Latest version!	Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu11875	NM_021292.2 Latest version!	Mus musculus EvC ciliary complex subunit 1 (Evc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$594.30 $849.00
View Old Accession Versions >>
OMu35151	XM_006504042.3	Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu69824	XM_011240761.2	Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu35152	XM_006504043.3	Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Evc ( XM_006504042.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504105.1 Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35151
Clone ID Related Accession (Same CDS sequence)	XM_006504042.3 , XM_006504042.4
Accession Version	XM_006504042.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ellis-van Creveld syndrome protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGCGGCGG AAGGCTGGAT CAGCAGGACA GCGAAGCTGG CGATGGAGAA 
AGCACAAGCA AGATACTGCA GAAAGGAAGC AATCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504105.1
CDS	452..3430
Translation	MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQSLRDTGSQAKARRRQRETTRDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAAAILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLCVCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLANTEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIVAEGLLHDSQDLHVLDTLERTMGRSHLARMVEFLRTQIQEETKCRLAAISRGLELLTVQGQLSGRQKEELLTQQHKAFWEEAERFGREFTQRGKDLVQASQARQAEAAAELTQTQEEERRSFLADSQLTSDPGEFLKAFHEVLERQRLTRSDQEGDEDTRITEAMAALCQELYCSTMGTFQKFVDSLFLKTLPEVTSLPVAECETLRQQVQEQAARQLGQADRFRRRQWGLLCDLLEQDKRVWLEEGTLSTVLQRQLRDHHESTIHGVLSRFSGLSEESSRGILQGHELLLCSALRRLALRGTTITALAQMRLSGKKRLLQELHEQLALEQGVSPCLEEHQWQLLRALEARIQEEAARLEDEAQQTGLRLQQQLLAEAQEAGRLLQLHMERVIGQALLVHARNVASKGRTREKEDFKRTLVETVVESVYVTSTSVNRLVQAHYQAVGKLLQAHEEQLLQRLKTLQGERINAYKLWKKQEFSDPSLESQTADGTHGASQGVQQRMLSQQKRLLDQFTKHQQGRLNSQRQKAQELDQLQAQLETQLQEAEQTLISELSTLARVPLPENKPFSNKRGLPEKPVRTKRKKPPPREREDLGTPNDDHLALADHTTGPLSGGRLDQQDSEAGDGESTSKILQKGSNL

Target ORF information:

RefSeq Version	XM_006504042.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504042.3

ORF Insert Sequence:

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGCGGCGG AAGGCTGGAT CAGCAGGACA GCGAAGCTGG CGATGGAGAA 
AGCACAAGCA AGATACTGCA GAAAGGAAGC AATCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc ( XM_006504043.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504106.1 Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu35152
Clone ID Related Accession (Same CDS sequence)	XM_006504043.3 , XM_006504043.4
Accession Version	XM_006504043.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ellis-van Creveld syndrome protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGC TTTTCATGAG GTCCTGGAGA GGCAGCGGCT GACAAGGAGT 
GACCAAGAGG GAGACGAGGA CACCCGAATC ACTGAGGCCA TGGCTGCGCT CTGCCAGGAG 
CTGTACTGTA GCACCATGGG AACCTTCCAA AAGTTTGTGG ACTCCCTGTT CCTGAAGACC 
CTCCCAGAAG TGACCAGCCT CCCTGTGGCA GAATGTGAGA CCCTGCGGCA GCAGGTCCAG 
GAGCAGGCAG CAAGGCAGCT GGGACAGGCA GACCGATTCC GCAGACGACA GTGGGGACTC 
CTATGTGATC TATTGGAGCA GGACAAACGG GTGTGGCTGG AGGAGGGTAC GCTGTCCACG 
GTGCTGCAGA GGCAGCTGCG GGATCACCAC GAGAGCACCA TCCACGGCGT CCTGAGCAGA 
TTCAGTGGCC TTTCTGAAGA GTCCTCACGA GGCATCCTGC AGGGCCACGA ACTGCTGCTG 
TGCTCTGCTC TGCGCAGACT GGCCCTCCGA GGCACCACCA TCACTGCCCT GGCTCAGATG 
AGGCTGTCTG GGAAGAAGAG GCTCCTGCAG GAGCTACATG AGCAGCTGGC ATTAGAGCAG 
GGTGTGTCCC CATGCCTGGA GGAGCATCAA TGGCAGCTGC TTAGAGCCCT GGAGGCGCGG 
ATCCAGGAGG AGGCAGCCAG GTTGGAGGAT GAGGCACAGC AGACAGGTCT GCGGCTCCAG 
CAGCAGCTGC TGGCGGAGGC ACAGGAGGCA GGGCGGCTGC TACAGCTGCA CATGGAGCGG 
GTCATTGGGC AGGCGCTGCT GGTACATGCT AGGAATGTTG CCAGCAAAGG CCGGACCAGG 
GAGAAGGAAG ACTTCAAGAG GACGCTGGTA GAGACGGTGG TGGAGAGTGT GTATGTGACC 
AGCACTAGTG TCAACCGCCT CGTGCAGGCG CATTACCAGG CCGTCGGGAA GCTCCTGCAG 
GCTCATGAGG AACAATTGCT GCAGCGCCTA AAGACCCTGC AGGGAGAGAG AATCAATGCT 
TACAAGCTGT GGAAGAAGCA AGAATTCAGT GACCCGTCGT TGGAGAGTCA AACAGCGGAT 
GGTACCCATG GAGCTTCCCA GGGGGTACAG CAGAGGATGC TTTCGCAGCA GAAGAGGCTC 
CTGGACCAGT TCACGAAGCA TCAGCAAGGC CGCCTGAACT CTCAGAGGCA GAAGGCACAA 
GAGCTGGACC AGCTGCAAGC CCAGCTTGAG ACCCAGCTGC AGGAAGCTGA GCAGACCCTT 
ATCTCAGAGC TATCGACATT GGCCCGAGTA CCCCTTCCTG AGAACAAGCC ATTCTCCAAC 
AAGCGAGGAC TTCCAGAAAA GCCAGTAAGA ACCAAAAGGA AGAAACCCCC ACCTCGGGAG 
AGAGAGGACC TGGGGACACC AAACGATGAC CACCTTGCCT TGGCCGACCA CACCACAGGA 
CCACTCAGTA CAACCTACTC AGCATCGCCT CCCATCCGTG TCCACAGCGG CGGAAGGCTG 
GATCAGCAGG ACAGCGAAGC TGGCGATGGA GAAAGCACAA GCAAGATACT GCAGAAAGGA 
AGCAATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504106.1
CDS	452..3103
Translation	MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQSLRDTGSQAKARRRQRETTRDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAAAILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLCVCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLANTEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIVAEGLLHDSQDLHVLAFHEVLERQRLTRSDQEGDEDTRITEAMAALCQELYCSTMGTFQKFVDSLFLKTLPEVTSLPVAECETLRQQVQEQAARQLGQADRFRRRQWGLLCDLLEQDKRVWLEEGTLSTVLQRQLRDHHESTIHGVLSRFSGLSEESSRGILQGHELLLCSALRRLALRGTTITALAQMRLSGKKRLLQELHEQLALEQGVSPCLEEHQWQLLRALEARIQEEAARLEDEAQQTGLRLQQQLLAEAQEAGRLLQLHMERVIGQALLVHARNVASKGRTREKEDFKRTLVETVVESVYVTSTSVNRLVQAHYQAVGKLLQAHEEQLLQRLKTLQGERINAYKLWKKQEFSDPSLESQTADGTHGASQGVQQRMLSQQKRLLDQFTKHQQGRLNSQRQKAQELDQLQAQLETQLQEAEQTLISELSTLARVPLPENKPFSNKRGLPEKPVRTKRKKPPPREREDLGTPNDDHLALADHTTGPLSTTYSASPPIRVHSGGRLDQQDSEAGDGESTSKILQKGSNL

Target ORF information:

RefSeq Version	XM_006504043.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504043.3

ORF Insert Sequence:

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGC TTTTCATGAG GTCCTGGAGA GGCAGCGGCT GACAAGGAGT 
GACCAAGAGG GAGACGAGGA CACCCGAATC ACTGAGGCCA TGGCTGCGCT CTGCCAGGAG 
CTGTACTGTA GCACCATGGG AACCTTCCAA AAGTTTGTGG ACTCCCTGTT CCTGAAGACC 
CTCCCAGAAG TGACCAGCCT CCCTGTGGCA GAATGTGAGA CCCTGCGGCA GCAGGTCCAG 
GAGCAGGCAG CAAGGCAGCT GGGACAGGCA GACCGATTCC GCAGACGACA GTGGGGACTC 
CTATGTGATC TATTGGAGCA GGACAAACGG GTGTGGCTGG AGGAGGGTAC GCTGTCCACG 
GTGCTGCAGA GGCAGCTGCG GGATCACCAC GAGAGCACCA TCCACGGCGT CCTGAGCAGA 
TTCAGTGGCC TTTCTGAAGA GTCCTCACGA GGCATCCTGC AGGGCCACGA ACTGCTGCTG 
TGCTCTGCTC TGCGCAGACT GGCCCTCCGA GGCACCACCA TCACTGCCCT GGCTCAGATG 
AGGCTGTCTG GGAAGAAGAG GCTCCTGCAG GAGCTACATG AGCAGCTGGC ATTAGAGCAG 
GGTGTGTCCC CATGCCTGGA GGAGCATCAA TGGCAGCTGC TTAGAGCCCT GGAGGCGCGG 
ATCCAGGAGG AGGCAGCCAG GTTGGAGGAT GAGGCACAGC AGACAGGTCT GCGGCTCCAG 
CAGCAGCTGC TGGCGGAGGC ACAGGAGGCA GGGCGGCTGC TACAGCTGCA CATGGAGCGG 
GTCATTGGGC AGGCGCTGCT GGTACATGCT AGGAATGTTG CCAGCAAAGG CCGGACCAGG 
GAGAAGGAAG ACTTCAAGAG GACGCTGGTA GAGACGGTGG TGGAGAGTGT GTATGTGACC 
AGCACTAGTG TCAACCGCCT CGTGCAGGCG CATTACCAGG CCGTCGGGAA GCTCCTGCAG 
GCTCATGAGG AACAATTGCT GCAGCGCCTA AAGACCCTGC AGGGAGAGAG AATCAATGCT 
TACAAGCTGT GGAAGAAGCA AGAATTCAGT GACCCGTCGT TGGAGAGTCA AACAGCGGAT 
GGTACCCATG GAGCTTCCCA GGGGGTACAG CAGAGGATGC TTTCGCAGCA GAAGAGGCTC 
CTGGACCAGT TCACGAAGCA TCAGCAAGGC CGCCTGAACT CTCAGAGGCA GAAGGCACAA 
GAGCTGGACC AGCTGCAAGC CCAGCTTGAG ACCCAGCTGC AGGAAGCTGA GCAGACCCTT 
ATCTCAGAGC TATCGACATT GGCCCGAGTA CCCCTTCCTG AGAACAAGCC ATTCTCCAAC 
AAGCGAGGAC TTCCAGAAAA GCCAGTAAGA ACCAAAAGGA AGAAACCCCC ACCTCGGGAG 
AGAGAGGACC TGGGGACACC AAACGATGAC CACCTTGCCT TGGCCGACCA CACCACAGGA 
CCACTCAGTA CAACCTACTC AGCATCGCCT CCCATCCGTG TCCACAGCGG CGGAAGGCTG 
GATCAGCAGG ACAGCGAAGC TGGCGATGGA GAAAGCACAA GCAAGATACT GCAGAAAGGA 
AGCAATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc ( XM_011240761.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239063.1 Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X3, mRNA.

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CloneID	OMu69824
Clone ID Related Accession (Same CDS sequence)	XM_011240761.3 , XM_011240761.2
Accession Version	XM_011240761.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ellis-van Creveld syndrome protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240761.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGAATATC CAAAAAGTGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239063.1
CDS	453..3365
Translation	MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQSLRDTGSQAKARRRQRETTRDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAAAILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLCVCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLANTEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIVAEGLLHDSQDLHVLDTLERTMGRSHLARMVEFLRTQIQEETKCRLAAISRGLELLTVQGQLSGRQKEELLTQQHKAFWEEAERFGREFTQRGKDLVQASQARQAEAAAELTQTQEEERRSFLADSQLTSDPGEFLKAFHEVLERQRLTRSDQEGDEDTRITEAMAALCQELYCSTMGTFQKFVDSLFLKTLPEVTSLPVAECETLRQQVQEQAARQLGQADRFRRRQWGLLCDLLEQDKRVWLEEGTLSTVLQRQLRDHHESTIHGVLSRFSGLSEESSRGILQGHELLLCSALRRLALRGTTITALAQMRLSGKKRLLQELHEQLALEQGVSPCLEEHQWQLLRALEARIQEEAARLEDEAQQTGLRLQQQLLAEAQEAGRLLQLHMERVIGQALLVHARNVASKGRTREKEDFKRTLVETVVESVYVTSTSVNRLVQAHYQAVGKLLQAHEEQLLQRLKTLQGERINAYKLWKKQEFSDPSLESQTADGTHGASQGVQQRMLSQQKRLLDQFTKHQQGRLNSQRQKAQELDQLQAQLETQLQEAEQTLISELSTLARVPLPENKPFSNKRGLPEKPVRTKRKKPPPREREDLGTPNDDHLALADHTTGPLRISKKC

Target ORF information:

RefSeq Version	XM_011240761.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240761.2

ORF Insert Sequence:

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGAATATC CAAAAAGTGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc ( XM_006504042.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504105.1 Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X1, mRNA.

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CloneID	OMu35151
Clone ID Related Accession (Same CDS sequence)	XM_006504042.3 , XM_006504042.4
Accession Version	XM_006504042.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ellis-van Creveld syndrome protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504042.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGCGGCGG AAGGCTGGAT CAGCAGGACA GCGAAGCTGG CGATGGAGAA 
AGCACAAGCA AGATACTGCA GAAAGGAAGC AATCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504105.1
CDS	153..3131
Translation	MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQSLRDTGSQAKARRRQRETTRDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAAAILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLCVCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLANTEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIVAEGLLHDSQDLHVLDTLERTMGRSHLARMVEFLRTQIQEETKCRLAAISRGLELLTVQGQLSGRQKEELLTQQHKAFWEEAERFGREFTQRGKDLVQASQARQAEAAAELTQTQEEERRSFLADSQLTSDPGEFLKAFHEVLERQRLTRSDQEGDEDTRITEAMAALCQELYCSTMGTFQKFVDSLFLKTLPEVTSLPVAECETLRQQVQEQAARQLGQADRFRRRQWGLLCDLLEQDKRVWLEEGTLSTVLQRQLRDHHESTIHGVLSRFSGLSEESSRGILQGHELLLCSALRRLALRGTTITALAQMRLSGKKRLLQELHEQLALEQGVSPCLEEHQWQLLRALEARIQEEAARLEDEAQQTGLRLQQQLLAEAQEAGRLLQLHMERVIGQALLVHARNVASKGRTREKEDFKRTLVETVVESVYVTSTSVNRLVQAHYQAVGKLLQAHEEQLLQRLKTLQGERINAYKLWKKQEFSDPSLESQTADGTHGASQGVQQRMLSQQKRLLDQFTKHQQGRLNSQRQKAQELDQLQAQLETQLQEAEQTLISELSTLARVPLPENKPFSNKRGLPEKPVRTKRKKPPPREREDLGTPNDDHLALADHTTGPLSGGRLDQQDSEAGDGESTSKILQKGSNL

Target ORF information:

RefSeq Version	XM_006504042.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504042.4

ORF Insert Sequence:

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGCGGCGG AAGGCTGGAT CAGCAGGACA GCGAAGCTGG CGATGGAGAA 
AGCACAAGCA AGATACTGCA GAAAGGAAGC AATCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc ( XM_006504043.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504106.1 Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X5, mRNA.

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CloneID	OMu35152
Clone ID Related Accession (Same CDS sequence)	XM_006504043.3 , XM_006504043.4
Accession Version	XM_006504043.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ellis-van Creveld syndrome protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504043.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGC TTTTCATGAG GTCCTGGAGA GGCAGCGGCT GACAAGGAGT 
GACCAAGAGG GAGACGAGGA CACCCGAATC ACTGAGGCCA TGGCTGCGCT CTGCCAGGAG 
CTGTACTGTA GCACCATGGG AACCTTCCAA AAGTTTGTGG ACTCCCTGTT CCTGAAGACC 
CTCCCAGAAG TGACCAGCCT CCCTGTGGCA GAATGTGAGA CCCTGCGGCA GCAGGTCCAG 
GAGCAGGCAG CAAGGCAGCT GGGACAGGCA GACCGATTCC GCAGACGACA GTGGGGACTC 
CTATGTGATC TATTGGAGCA GGACAAACGG GTGTGGCTGG AGGAGGGTAC GCTGTCCACG 
GTGCTGCAGA GGCAGCTGCG GGATCACCAC GAGAGCACCA TCCACGGCGT CCTGAGCAGA 
TTCAGTGGCC TTTCTGAAGA GTCCTCACGA GGCATCCTGC AGGGCCACGA ACTGCTGCTG 
TGCTCTGCTC TGCGCAGACT GGCCCTCCGA GGCACCACCA TCACTGCCCT GGCTCAGATG 
AGGCTGTCTG GGAAGAAGAG GCTCCTGCAG GAGCTACATG AGCAGCTGGC ATTAGAGCAG 
GGTGTGTCCC CATGCCTGGA GGAGCATCAA TGGCAGCTGC TTAGAGCCCT GGAGGCGCGG 
ATCCAGGAGG AGGCAGCCAG GTTGGAGGAT GAGGCACAGC AGACAGGTCT GCGGCTCCAG 
CAGCAGCTGC TGGCGGAGGC ACAGGAGGCA GGGCGGCTGC TACAGCTGCA CATGGAGCGG 
GTCATTGGGC AGGCGCTGCT GGTACATGCT AGGAATGTTG CCAGCAAAGG CCGGACCAGG 
GAGAAGGAAG ACTTCAAGAG GACGCTGGTA GAGACGGTGG TGGAGAGTGT GTATGTGACC 
AGCACTAGTG TCAACCGCCT CGTGCAGGCG CATTACCAGG CCGTCGGGAA GCTCCTGCAG 
GCTCATGAGG AACAATTGCT GCAGCGCCTA AAGACCCTGC AGGGAGAGAG AATCAATGCT 
TACAAGCTGT GGAAGAAGCA AGAATTCAGT GACCCGTCGT TGGAGAGTCA AACAGCGGAT 
GGTACCCATG GAGCTTCCCA GGGGGTACAG CAGAGGATGC TTTCGCAGCA GAAGAGGCTC 
CTGGACCAGT TCACGAAGCA TCAGCAAGGC CGCCTGAACT CTCAGAGGCA GAAGGCACAA 
GAGCTGGACC AGCTGCAAGC CCAGCTTGAG ACCCAGCTGC AGGAAGCTGA GCAGACCCTT 
ATCTCAGAGC TATCGACATT GGCCCGAGTA CCCCTTCCTG AGAACAAGCC ATTCTCCAAC 
AAGCGAGGAC TTCCAGAAAA GCCAGTAAGA ACCAAAAGGA AGAAACCCCC ACCTCGGGAG 
AGAGAGGACC TGGGGACACC AAACGATGAC CACCTTGCCT TGGCCGACCA CACCACAGGA 
CCACTCAGTA CAACCTACTC AGCATCGCCT CCCATCCGTG TCCACAGCGG CGGAAGGCTG 
GATCAGCAGG ACAGCGAAGC TGGCGATGGA GAAAGCACAA GCAAGATACT GCAGAAAGGA 
AGCAATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504106.1
CDS	153..2804
Translation	MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQSLRDTGSQAKARRRQRETTRDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAAAILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLCVCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLANTEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIVAEGLLHDSQDLHVLAFHEVLERQRLTRSDQEGDEDTRITEAMAALCQELYCSTMGTFQKFVDSLFLKTLPEVTSLPVAECETLRQQVQEQAARQLGQADRFRRRQWGLLCDLLEQDKRVWLEEGTLSTVLQRQLRDHHESTIHGVLSRFSGLSEESSRGILQGHELLLCSALRRLALRGTTITALAQMRLSGKKRLLQELHEQLALEQGVSPCLEEHQWQLLRALEARIQEEAARLEDEAQQTGLRLQQQLLAEAQEAGRLLQLHMERVIGQALLVHARNVASKGRTREKEDFKRTLVETVVESVYVTSTSVNRLVQAHYQAVGKLLQAHEEQLLQRLKTLQGERINAYKLWKKQEFSDPSLESQTADGTHGASQGVQQRMLSQQKRLLDQFTKHQQGRLNSQRQKAQELDQLQAQLETQLQEAEQTLISELSTLARVPLPENKPFSNKRGLPEKPVRTKRKKPPPREREDLGTPNDDHLALADHTTGPLSTTYSASPPIRVHSGGRLDQQDSEAGDGESTSKILQKGSNL

Target ORF information:

RefSeq Version	XM_006504043.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504043.4

ORF Insert Sequence:

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGC TTTTCATGAG GTCCTGGAGA GGCAGCGGCT GACAAGGAGT 
GACCAAGAGG GAGACGAGGA CACCCGAATC ACTGAGGCCA TGGCTGCGCT CTGCCAGGAG 
CTGTACTGTA GCACCATGGG AACCTTCCAA AAGTTTGTGG ACTCCCTGTT CCTGAAGACC 
CTCCCAGAAG TGACCAGCCT CCCTGTGGCA GAATGTGAGA CCCTGCGGCA GCAGGTCCAG 
GAGCAGGCAG CAAGGCAGCT GGGACAGGCA GACCGATTCC GCAGACGACA GTGGGGACTC 
CTATGTGATC TATTGGAGCA GGACAAACGG GTGTGGCTGG AGGAGGGTAC GCTGTCCACG 
GTGCTGCAGA GGCAGCTGCG GGATCACCAC GAGAGCACCA TCCACGGCGT CCTGAGCAGA 
TTCAGTGGCC TTTCTGAAGA GTCCTCACGA GGCATCCTGC AGGGCCACGA ACTGCTGCTG 
TGCTCTGCTC TGCGCAGACT GGCCCTCCGA GGCACCACCA TCACTGCCCT GGCTCAGATG 
AGGCTGTCTG GGAAGAAGAG GCTCCTGCAG GAGCTACATG AGCAGCTGGC ATTAGAGCAG 
GGTGTGTCCC CATGCCTGGA GGAGCATCAA TGGCAGCTGC TTAGAGCCCT GGAGGCGCGG 
ATCCAGGAGG AGGCAGCCAG GTTGGAGGAT GAGGCACAGC AGACAGGTCT GCGGCTCCAG 
CAGCAGCTGC TGGCGGAGGC ACAGGAGGCA GGGCGGCTGC TACAGCTGCA CATGGAGCGG 
GTCATTGGGC AGGCGCTGCT GGTACATGCT AGGAATGTTG CCAGCAAAGG CCGGACCAGG 
GAGAAGGAAG ACTTCAAGAG GACGCTGGTA GAGACGGTGG TGGAGAGTGT GTATGTGACC 
AGCACTAGTG TCAACCGCCT CGTGCAGGCG CATTACCAGG CCGTCGGGAA GCTCCTGCAG 
GCTCATGAGG AACAATTGCT GCAGCGCCTA AAGACCCTGC AGGGAGAGAG AATCAATGCT 
TACAAGCTGT GGAAGAAGCA AGAATTCAGT GACCCGTCGT TGGAGAGTCA AACAGCGGAT 
GGTACCCATG GAGCTTCCCA GGGGGTACAG CAGAGGATGC TTTCGCAGCA GAAGAGGCTC 
CTGGACCAGT TCACGAAGCA TCAGCAAGGC CGCCTGAACT CTCAGAGGCA GAAGGCACAA 
GAGCTGGACC AGCTGCAAGC CCAGCTTGAG ACCCAGCTGC AGGAAGCTGA GCAGACCCTT 
ATCTCAGAGC TATCGACATT GGCCCGAGTA CCCCTTCCTG AGAACAAGCC ATTCTCCAAC 
AAGCGAGGAC TTCCAGAAAA GCCAGTAAGA ACCAAAAGGA AGAAACCCCC ACCTCGGGAG 
AGAGAGGACC TGGGGACACC AAACGATGAC CACCTTGCCT TGGCCGACCA CACCACAGGA 
CCACTCAGTA CAACCTACTC AGCATCGCCT CCCATCCGTG TCCACAGCGG CGGAAGGCTG 
GATCAGCAGG ACAGCGAAGC TGGCGATGGA GAAAGCACAA GCAAGATACT GCAGAAAGGA 
AGCAATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc ( XM_011240761.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239063.1 Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X2, mRNA.

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CloneID	OMu69824
Clone ID Related Accession (Same CDS sequence)	XM_011240761.3 , XM_011240761.2
Accession Version	XM_011240761.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ellis-van Creveld syndrome protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240761.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGAATATC CAAAAAGTGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239063.1
CDS	154..3066
Translation	MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQSLRDTGSQAKARRRQRETTRDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAAAILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLCVCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLANTEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIVAEGLLHDSQDLHVLDTLERTMGRSHLARMVEFLRTQIQEETKCRLAAISRGLELLTVQGQLSGRQKEELLTQQHKAFWEEAERFGREFTQRGKDLVQASQARQAEAAAELTQTQEEERRSFLADSQLTSDPGEFLKAFHEVLERQRLTRSDQEGDEDTRITEAMAALCQELYCSTMGTFQKFVDSLFLKTLPEVTSLPVAECETLRQQVQEQAARQLGQADRFRRRQWGLLCDLLEQDKRVWLEEGTLSTVLQRQLRDHHESTIHGVLSRFSGLSEESSRGILQGHELLLCSALRRLALRGTTITALAQMRLSGKKRLLQELHEQLALEQGVSPCLEEHQWQLLRALEARIQEEAARLEDEAQQTGLRLQQQLLAEAQEAGRLLQLHMERVIGQALLVHARNVASKGRTREKEDFKRTLVETVVESVYVTSTSVNRLVQAHYQAVGKLLQAHEEQLLQRLKTLQGERINAYKLWKKQEFSDPSLESQTADGTHGASQGVQQRMLSQQKRLLDQFTKHQQGRLNSQRQKAQELDQLQAQLETQLQEAEQTLISELSTLARVPLPENKPFSNKRGLPEKPVRTKRKKPPPREREDLGTPNDDHLALADHTTGPLRISKKC

Target ORF information:

RefSeq Version	XM_011240761.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EvC ciliary complex subunit 1 (Evc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240761.3

ORF Insert Sequence:

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGAATATC CAAAAAGTGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc ( XM_017321049.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176538.1 Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X6, mRNA.

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CloneID	OMu81459
Clone ID Related Accession (Same CDS sequence)	XM_017321049.1
Accession Version	XM_017321049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ellis-van Creveld syndrome protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGCTCTTTG CTTGTCTTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176538.1
CDS	838..2421
Translation	MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQSLRDTGSQAKARRRQRETTRDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAAAILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLCVCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLANTEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIVAEGLLHDSQDLHVLDTLERTMGRSHLARMVEFLRTQIQEETKCRLAAISRGLELLTVQGQLSGRQKEELLTQQHKAFWEEAERFGREFTQRGKDLVQASQARQAEAAAELTQTQEEERRSFLADSQLTSDPGEFLKAFHEVLERQRLTRSDQEGDEDTRITEAMAALCQLFACLV

Target ORF information:

RefSeq Version	XM_017321049.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ellis van Creveld gene syndrome (Evc), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321049.1

ORF Insert Sequence:

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGCTCTTTG CTTGTCTTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc ( NM_021292.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067267.2 Mus musculus EvC ciliary complex subunit 1 (Evc), mRNA.

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CloneID	OMu11875
Clone ID Related Accession (Same CDS sequence)	NM_021292.2
Accession Version	NM_021292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ellis-van Creveld syndrome protein homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY242128.1, AK039759.1, AJ250841.1 and AA423362.1. On Nov 21, 2009 this sequence version replaced NM_021292.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ250841.1, AK148262.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGTACAAC CTACTCAGCA TCGCCTCCCA TCCGTGTCCA CAGCGGCGGA 
AGGCTGGATC AGCAGGACAG CGAAGCTGGC GATGGAGAAA GCACAAGCAA GATACTGCAG 
AAAGGAAGCA ATCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067267.2
CDS	89..3106
Translation	MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQSLRDTGSQAKARRRQRETTRDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAAAILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLCVCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLANTEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIVAEGLLHDSQDLHVLDTLERTMGRSHLARMVEFLRTQIQEETKCRLAAISRGLELLTVQGQLSGRQKEELLTQQHKAFWEEAERFGREFTQRGKDLVQASQARQAEAAAELTQTQEEERRSFLADSQLTSDPGEFLKAFHEVLERQRLTRSDQEGDEDTRITEAMAALCQELYCSTMGTFQKFVDSLFLKTLPEVTSLPVAECETLRQQVQEQAARQLGQADRFRRRQWGLLCDLLEQDKRVWLEEGTLSTVLQRQLRDHHESTIHGVLSRFSGLSEESSRGILQGHELLLCSALRRLALRGTTITALAQMRLSGKKRLLQELHEQLALEQGVSPCLEEHQWQLLRALEARIQEEAARLEDEAQQTGLRLQQQLLAEAQEAGRLLQLHMERVIGQALLVHARNVASKGRTREKEDFKRTLVETVVESVYVTSTSVNRLVQAHYQAVGKLLQAHEEQLLQRLKTLQGERINAYKLWKKQEFSDPSLESQTADGTHGASQGVQQRMLSQQKRLLDQFTKHQQGRLNSQRQKAQELDQLQAQLETQLQEAEQTLISELSTLARVPLPENKPFSNKRGLPEKPVRTKRKKPPPREREDLGTPNDDHLALADHTTGPLSTTYSASPPIRVHSGGRLDQQDSEAGDGESTSKILQKGSNL

Target ORF information:

RefSeq Version	NM_021292.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus EvC ciliary complex subunit 1 (Evc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021292.2

ORF Insert Sequence:

ATGACCTGCA CTAAAGATGC GCGGCTGCAG CTGGGCCGGG AAGCCCTGCA GGCGGCGCCG 
ACGCTGCTGG TCCCCGCGGT GCTACTGGGT GGCGTTCTCG GCCTAGGCCT TGGCTTGTGG 
CTTGGCTGTC GCGCGAGCCA CTTGCGCGCA CGGCTCCAGA AAGATGACAG GAAACGTCTG 
CTCGGGAGTT CAGAGCCCCC TGCACAGAGC CTACGGGACA CTGGCTCTCA AGCCAAAGCC 
AGGAGGCGCC AGAGAGAAAC CACTCGGGAT GAAGACGCCC CTGAGGTGTG TGAGCCATCC 
TTGAGTGGCA ACATCACGGC ATTTGCACTG AAAGCCAGAG TTGTCTATCC CATCAACCAG 
AAGTTCCGGC CTCTGGCCGA CGGATCCTCC CACCCATCGC TGCATGAAAA CCTGACACAG 
GCAGCGGCCA TCCTGCCCCA CCTGCCCCAC CAGCCAGCAG AGGCCTCACC AGCCAGCAGC 
CTGGGCAGCC TGAGCCAGGC TGGCAAGGAG GACGGCAGCT CCTCCTCCAG CATGCGGTCA 
ACCTACAGCG ACGACCGGAT CCTCCAGTGT GCCTTTCTCC GGGTGGGCAG CTTCCCTGAG 
ATCCTGGCCT GTGAGAGTGT GGACATTGAC CTGTGTGTCT GCAGCCTTCA CCTGAAAGAC 
CTGCTTCAGG TAGACACGGC GCTGAGGCAA GAGAAGCACC TGATGTTCAT TCAGATTCTT 
AAAGCATGCC TCCTGGATTT TTTTCCTAAA AAGAAGCCTG ATGATGAATT ATGCCAGAAG 
GTTCTTTCCA AACAAGAGCA TGATTTGGAG GAATTAGAAA AGGGACTCCA GGCCAGATTG 
GCGAACACAG AAATGTTAGG CACTGGCGAC TCTGGCTACG TTTCCCTGGC AGATGTGGAG 
AGGAAGGAGA GAGAGCTCTC AGAGCAGCTC ATAGACAACA TGGGAGCTTT CTGGAAGCAG 
ATGGAAAGCA TCCAGCCCAC TCTCATGGAC CAGTTTAAGT GTTCCAGCTC CAAAGCTCGG 
CAGTTCATGA TGACTCTGAC AGGAAGAATG ATTGTCGCTG AAGGACTGCT GCATGACTCT 
CAGGACCTGC ATGTCCTGGA CACCCTGGAG AGGACAATGG GTCGATCCCA CTTGGCAAGA 
ATGGTGGAGT TCCTGAGAAC CCAGATCCAG GAGGAGACCA AGTGCAGACT TGCTGCCATC 
TCCCGTGGCC TGGAGTTGCT GACTGTCCAG GGGCAGCTGT CTGGGAGGCA AAAGGAGGAA 
CTCCTGACTC AGCAGCACAA GGCCTTCTGG GAGGAGGCAG AGCGTTTTGG CAGGGAATTC 
ACGCAGCGGG GCAAAGATCT GGTCCAGGCG TCTCAGGCTC GCCAGGCAGA GGCAGCAGCA 
GAGCTCACAC AGACCCAAGA AGAAGAACGG AGGAGTTTCC TGGCTGATTC CCAGCTGACC 
TCGGATCCTG GGGAATTCCT CAAGGCTTTT CATGAGGTCC TGGAGAGGCA GCGGCTGACA 
AGGAGTGACC AAGAGGGAGA CGAGGACACC CGAATCACTG AGGCCATGGC TGCGCTCTGC 
CAGGAGCTGT ACTGTAGCAC CATGGGAACC TTCCAAAAGT TTGTGGACTC CCTGTTCCTG 
AAGACCCTCC CAGAAGTGAC CAGCCTCCCT GTGGCAGAAT GTGAGACCCT GCGGCAGCAG 
GTCCAGGAGC AGGCAGCAAG GCAGCTGGGA CAGGCAGACC GATTCCGCAG ACGACAGTGG 
GGACTCCTAT GTGATCTATT GGAGCAGGAC AAACGGGTGT GGCTGGAGGA GGGTACGCTG 
TCCACGGTGC TGCAGAGGCA GCTGCGGGAT CACCACGAGA GCACCATCCA CGGCGTCCTG 
AGCAGATTCA GTGGCCTTTC TGAAGAGTCC TCACGAGGCA TCCTGCAGGG CCACGAACTG 
CTGCTGTGCT CTGCTCTGCG CAGACTGGCC CTCCGAGGCA CCACCATCAC TGCCCTGGCT 
CAGATGAGGC TGTCTGGGAA GAAGAGGCTC CTGCAGGAGC TACATGAGCA GCTGGCATTA 
GAGCAGGGTG TGTCCCCATG CCTGGAGGAG CATCAATGGC AGCTGCTTAG AGCCCTGGAG 
GCGCGGATCC AGGAGGAGGC AGCCAGGTTG GAGGATGAGG CACAGCAGAC AGGTCTGCGG 
CTCCAGCAGC AGCTGCTGGC GGAGGCACAG GAGGCAGGGC GGCTGCTACA GCTGCACATG 
GAGCGGGTCA TTGGGCAGGC GCTGCTGGTA CATGCTAGGA ATGTTGCCAG CAAAGGCCGG 
ACCAGGGAGA AGGAAGACTT CAAGAGGACG CTGGTAGAGA CGGTGGTGGA GAGTGTGTAT 
GTGACCAGCA CTAGTGTCAA CCGCCTCGTG CAGGCGCATT ACCAGGCCGT CGGGAAGCTC 
CTGCAGGCTC ATGAGGAACA ATTGCTGCAG CGCCTAAAGA CCCTGCAGGG AGAGAGAATC 
AATGCTTACA AGCTGTGGAA GAAGCAAGAA TTCAGTGACC CGTCGTTGGA GAGTCAAACA 
GCGGATGGTA CCCATGGAGC TTCCCAGGGG GTACAGCAGA GGATGCTTTC GCAGCAGAAG 
AGGCTCCTGG ACCAGTTCAC GAAGCATCAG CAAGGCCGCC TGAACTCTCA GAGGCAGAAG 
GCACAAGAGC TGGACCAGCT GCAAGCCCAG CTTGAGACCC AGCTGCAGGA AGCTGAGCAG 
ACCCTTATCT CAGAGCTATC GACATTGGCC CGAGTACCCC TTCCTGAGAA CAAGCCATTC 
TCCAACAAGC GAGGACTTCC AGAAAAGCCA GTAAGAACCA AAAGGAAGAA ACCCCCACCT 
CGGGAGAGAG AGGACCTGGG GACACCAAAC GATGACCACC TTGCCTTGGC CGACCACACC 
ACAGGACCAC TCAGTACAAC CTACTCAGCA TCGCCTCCCA TCCGTGTCCA CAGCGGCGGA 
AGGCTGGATC AGCAGGACAG CGAAGCTGGC GATGGAGAAA GCACAAGCAA GATACTGCAG 
AAAGGAAGCA ATCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Human molecular genetics25(14)3011-3028(2016 07)
Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

EFCAB7 and IQCE regulate hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia.
Developmental cell28(5)483-96(2014 Mar)
Pusapati GV,Hughes CE,Dorn KV,Zhang D,Sugianto P,Aravind L,Rohatgi R

Evc regulates a symmetrical response to Shh signaling in molar development.
Journal of dental research92(3)222-8(2013 Mar)
Nakatomi M,Hovorakova M,Gritli-Linde A,Blair HJ,MacArthur K,Peterka M,Lesot H,Peterkova R,Ruiz-Perez VL,Goodship JA,Peters H

The ciliary Evc/Evc2 complex interacts with Smo and controls Hedgehog pathway activity in chondrocytes by regulating Sufu/Gli3 dissociation and Gli3 trafficking in primary cilia.
Human molecular genetics22(1)124-39(2013 Jan)
Caparr?s-Mart?n JA,Valencia M,Reytor E,Pacheco M,Fernandez M,Perez-Aytes A,Gean E,Lapunzina P,Peters H,Goodship JA,Ruiz-Perez VL

Smoothened transduces Hedgehog signal by forming a complex with Evc/Evc2.
Cell research22(11)1593-604(2012 Nov)
Yang C,Chen W,Chen Y,Jiang J

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Evc cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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