Chst12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chst12
Entrez Gene ID	59031
Full Name	carbohydrate sulfotransferase 12
Synonyms	AI595374,C4S-2,C4ST2
General protein information	Preferred Names carbohydrate sulfotransferase 12 Names carbohydrate sulfotransferase 12 chondroitin 4-O-sulfotransferase 2 chondroitin 4-sulfotransferase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006504725.2	XP_006504788.1	carbohydrate sulfotransferase 12 isoform X1
NM_021528.3	NP_067503.3	carbohydrate sulfotransferase 12
XM_006504725.3	XP_006504788.1	carbohydrate sulfotransferase 12 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CHST12	NP_001071381.1
Rattus norvegicus (Norway rat)	Chst12	NP_001032864.1
Homo sapiens (human)	CHST12	NP_061111.1
	CHST12	XP_001103002.2
Gallus gallus (chicken)	CHST12	XP_004945197.1
Mus musculus (house mouse)	Chst12	NP_067503.3
Pan troglodytes (chimpanzee)	CHST12	XP_003318272.1
Canis lupus familiaris (dog)	CHST12	XP_005621219.1
Danio rerio (zebrafish)	chst12a	NP_998712.2
Xenopus tropicalis (tropical clawed frog)	chst12	NP_001015949.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.
Nakahara M, Tateyama H, Araki M, Nakagata N, Yamamura K, Araki K
Mammalian genome : official journal of the International Mammalian Genome Society24(5-6)228-39(2013 Jun)

Chondroitin 4-O-sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1.
Izumikawa T, Koike T, Kitagawa H
The Biochemical journal441(2)697-705(2012 Jan)

Expression of multiple chondroitin/dermatan sulfotransferases in the neurogenic regions of the embryonic and adult central nervous system implies that complex chondroitin sulfates have a role in neural stem cell maintenance.
Akita K, von Holst A, Furukawa Y, Mikami T, Sugahara K, Faissner A
Stem cells (Dayton, Ohio)26(3)798-809(2008 Mar)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

Exchangeable gene trap using the Cre/mutated lox system.
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

chondroitin 4-O-sulfotransferase-2 efficiently transferred sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of non-reducing terminal GalNAc-linkage residues forming chondroitin sulfate.
Title: Chondroitin 4-O-sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1.

The following Chst12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chst12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12449	NM_021528.3 Latest version!	Mus musculus carbohydrate sulfotransferase 12 (Chst12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu12449	XM_006504725.3 Latest version!	Mus musculus carbohydrate sulfotransferase 12 (Chst12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu12449	XM_006504725.2	Mus musculus carbohydrate sulfotransferase 12 (Chst12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chst12 ( NM_021528.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067503.3 Mus musculus carbohydrate sulfotransferase 12 (Chst12), mRNA.

Price & Availability↑

CloneID	OMu12449
Clone ID Related Accession (Same CDS sequence)	XM_006504725.2 , NM_021528.3 , XM_006504725.3
Accession Version	NM_021528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Mus musculus(house mouse)
Product	carbohydrate sulfotransferase 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB868264.1, AK142155.1 and AJ289132.1. On Sep 14, 2007 this sequence version replaced NM_021528.2. ##Evidence-Data-START## Transcript exon combination :: BC003201.1, AK142155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAAGC CGCGGCTCTT CCGGCTGTGG CTGGTACTAG GGTCGGCTCT CATGATCCTT 
TTGATCATTG TATATTGGGA CAACGTGGGC ACCGCCCACT TCTATCTGCA CACGTCTCTC 
TCCAGGCCAC ACATCCTAGA ACCCCTTCCC ACCCAGGGAT TGGTGGAGGA GAACGTGTTC 
ACATCCGACG TGGATGAGTT TTTGGATACT CTCCTTAGTT CTGACGCAAA GCACAACGAC 
CTTTCCAGGA GAAAAACTGA GCAGCCCCCG GTGCCCGCCC CCAGCAAGCC AGTCTTGAGC 
CACATGGAGG AGAACGTGAG AGGCTACGAC TGGTCCACTC ATGATGCCCA TCAGAACCCT 
GACCGGGACA GGCAGCAGGC CGAGAGGAGG AGCCTGCTGA GAGACTTCTG TGCCAACGCC 
AGCCTGGCAT TCCCCACCAA GGACCGCTCT TTTGACGACA TCCCCAACTA CGAACTGAAC 
CACCTGATCG TGGACGACCG CCACGGGGTC ATCTACTGCT ACGTGCCCAA GGTGGCCTGC 
ACCAACTGGA AGCGAGTGAT GATCGTGCTG AGCGAGAGCC TGCTGGACCG GGGCAGCCCC 
TACCGAGACC CCCTGGACAT CCCCCGGGAA CACGTGCACA ACACCAGCAC GCACCTCACC 
TTCAACAAGT TCTGGCGCCG CTACGGAAAG TTCTCCCGTC ACCTAATGAA GGTGAAGCTG 
AAGAAGTACA CCAAGTTCCT GTTCGTGCGC GACCCCTTTG TGCGCCTCAT CTCAGCCTTC 
CGCAGCAAGT TCGAGCTGGA GAACGAAGAG TTTTACCGCA AGTTCGCGGT GCCCATGCTC 
CGACTGTACG CCAACCACAC CAGCCTGCCC GCCTCGGTGA GTGAGGCTTT CAGCGCCGGG 
CTCAAGGTCT CCTTCGCCAA CTTCATCCAG TACCTCCTGG ACCCACACAC GGAGAAGCTG 
GCGCCTTTCA ACGAGCACTG GCGACAGGTG TACCGCCTCT GCCACCCGTG CCAGATAGAC 
TATGACTTCG TGGGGAAGCT GGAGACGCTC GATGAGGACG CTGCCCAGCT CCTGAGGTTC 
CTCAAGGTAG ACTCCCAGCT CCACTTCCCC CCCAGTTATC GGAACAGGAC GGCCAGCAGC 
TGGGAGGAAG ACTGGTTTGC CAACATCCCC CTGGCATGGA GGCAACAGCT CTATAAACTC 
TACGAGGCCG ACTTTGTTCT CTTTGGCTAC CCCAAGCCAG AAAACCTGCT CAGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067503.3
CDS	168..1427
Translation	MTKPRLFRLWLVLGSALMILLIIVYWDNVGTAHFYLHTSLSRPHILEPLPTQGLVEENVFTSDVDEFLDTLLSSDAKHNDLSRRKTEQPPVPAPSKPVLSHMEENVRGYDWSTHDAHQNPDRDRQQAERRSLLRDFCANASLAFPTKDRSFDDIPNYELNHLIVDDRHGVIYCYVPKVACTNWKRVMIVLSESLLDRGSPYRDPLDIPREHVHNTSTHLTFNKFWRRYGKFSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLRLYANHTSLPASVSEAFSAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQIDYDFVGKLETLDEDAAQLLRFLKVDSQLHFPPSYRNRTASSWEEDWFANIPLAWRQQLYKLYEADFVLFGYPKPENLLRD

Target ORF information:

RefSeq Version	NM_021528.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbohydrate sulfotransferase 12 (Chst12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021528.3

ORF Insert Sequence:

ATGACCAAGC CGCGGCTCTT CCGGCTGTGG CTGGTACTAG GGTCGGCTCT CATGATCCTT 
TTGATCATTG TATATTGGGA CAACGTGGGC ACCGCCCACT TCTATCTGCA CACGTCTCTC 
TCCAGGCCAC ACATCCTAGA ACCCCTTCCC ACCCAGGGAT TGGTGGAGGA GAACGTGTTC 
ACATCCGACG TGGATGAGTT TTTGGATACT CTCCTTAGTT CTGACGCAAA GCACAACGAC 
CTTTCCAGGA GAAAAACTGA GCAGCCCCCG GTGCCCGCCC CCAGCAAGCC AGTCTTGAGC 
CACATGGAGG AGAACGTGAG AGGCTACGAC TGGTCCACTC ATGATGCCCA TCAGAACCCT 
GACCGGGACA GGCAGCAGGC CGAGAGGAGG AGCCTGCTGA GAGACTTCTG TGCCAACGCC 
AGCCTGGCAT TCCCCACCAA GGACCGCTCT TTTGACGACA TCCCCAACTA CGAACTGAAC 
CACCTGATCG TGGACGACCG CCACGGGGTC ATCTACTGCT ACGTGCCCAA GGTGGCCTGC 
ACCAACTGGA AGCGAGTGAT GATCGTGCTG AGCGAGAGCC TGCTGGACCG GGGCAGCCCC 
TACCGAGACC CCCTGGACAT CCCCCGGGAA CACGTGCACA ACACCAGCAC GCACCTCACC 
TTCAACAAGT TCTGGCGCCG CTACGGAAAG TTCTCCCGTC ACCTAATGAA GGTGAAGCTG 
AAGAAGTACA CCAAGTTCCT GTTCGTGCGC GACCCCTTTG TGCGCCTCAT CTCAGCCTTC 
CGCAGCAAGT TCGAGCTGGA GAACGAAGAG TTTTACCGCA AGTTCGCGGT GCCCATGCTC 
CGACTGTACG CCAACCACAC CAGCCTGCCC GCCTCGGTGA GTGAGGCTTT CAGCGCCGGG 
CTCAAGGTCT CCTTCGCCAA CTTCATCCAG TACCTCCTGG ACCCACACAC GGAGAAGCTG 
GCGCCTTTCA ACGAGCACTG GCGACAGGTG TACCGCCTCT GCCACCCGTG CCAGATAGAC 
TATGACTTCG TGGGGAAGCT GGAGACGCTC GATGAGGACG CTGCCCAGCT CCTGAGGTTC 
CTCAAGGTAG ACTCCCAGCT CCACTTCCCC CCCAGTTATC GGAACAGGAC GGCCAGCAGC 
TGGGAGGAAG ACTGGTTTGC CAACATCCCC CTGGCATGGA GGCAACAGCT CTATAAACTC 
TACGAGGCCG ACTTTGTTCT CTTTGGCTAC CCCAAGCCAG AAAACCTGCT CAGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chst12 ( XM_006504725.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504788.1 Mus musculus carbohydrate sulfotransferase 12 (Chst12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12449
Clone ID Related Accession (Same CDS sequence)	XM_006504725.2 , NM_021528.3 , XM_006504725.3
Accession Version	XM_006504725.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	carbohydrate sulfotransferase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006504725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC CGCGGCTCTT CCGGCTGTGG CTGGTACTAG GGTCGGCTCT CATGATCCTT 
TTGATCATTG TATATTGGGA CAACGTGGGC ACCGCCCACT TCTATCTGCA CACGTCTCTC 
TCCAGGCCAC ACATCCTAGA ACCCCTTCCC ACCCAGGGAT TGGTGGAGGA GAACGTGTTC 
ACATCCGACG TGGATGAGTT TTTGGATACT CTCCTTAGTT CTGACGCAAA GCACAACGAC 
CTTTCCAGGA GAAAAACTGA GCAGCCCCCG GTGCCCGCCC CCAGCAAGCC AGTCTTGAGC 
CACATGGAGG AGAACGTGAG AGGCTACGAC TGGTCCACTC ATGATGCCCA TCAGAACCCT 
GACCGGGACA GGCAGCAGGC CGAGAGGAGG AGCCTGCTGA GAGACTTCTG TGCCAACGCC 
AGCCTGGCAT TCCCCACCAA GGACCGCTCT TTTGACGACA TCCCCAACTA CGAACTGAAC 
CACCTGATCG TGGACGACCG CCACGGGGTC ATCTACTGCT ACGTGCCCAA GGTGGCCTGC 
ACCAACTGGA AGCGAGTGAT GATCGTGCTG AGCGAGAGCC TGCTGGACCG GGGCAGCCCC 
TACCGAGACC CCCTGGACAT CCCCCGGGAA CACGTGCACA ACACCAGCAC GCACCTCACC 
TTCAACAAGT TCTGGCGCCG CTACGGAAAG TTCTCCCGTC ACCTAATGAA GGTGAAGCTG 
AAGAAGTACA CCAAGTTCCT GTTCGTGCGC GACCCCTTTG TGCGCCTCAT CTCAGCCTTC 
CGCAGCAAGT TCGAGCTGGA GAACGAAGAG TTTTACCGCA AGTTCGCGGT GCCCATGCTC 
CGACTGTACG CCAACCACAC CAGCCTGCCC GCCTCGGTGA GTGAGGCTTT CAGCGCCGGG 
CTCAAGGTCT CCTTCGCCAA CTTCATCCAG TACCTCCTGG ACCCACACAC GGAGAAGCTG 
GCGCCTTTCA ACGAGCACTG GCGACAGGTG TACCGCCTCT GCCACCCGTG CCAGATAGAC 
TATGACTTCG TGGGGAAGCT GGAGACGCTC GATGAGGACG CTGCCCAGCT CCTGAGGTTC 
CTCAAGGTAG ACTCCCAGCT CCACTTCCCC CCCAGTTATC GGAACAGGAC GGCCAGCAGC 
TGGGAGGAAG ACTGGTTTGC CAACATCCCC CTGGCATGGA GGCAACAGCT CTATAAACTC 
TACGAGGCCG ACTTTGTTCT CTTTGGCTAC CCCAAGCCAG AAAACCTGCT CAGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504788.1
CDS	228..1487
Translation	MTKPRLFRLWLVLGSALMILLIIVYWDNVGTAHFYLHTSLSRPHILEPLPTQGLVEENVFTSDVDEFLDTLLSSDAKHNDLSRRKTEQPPVPAPSKPVLSHMEENVRGYDWSTHDAHQNPDRDRQQAERRSLLRDFCANASLAFPTKDRSFDDIPNYELNHLIVDDRHGVIYCYVPKVACTNWKRVMIVLSESLLDRGSPYRDPLDIPREHVHNTSTHLTFNKFWRRYGKFSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLRLYANHTSLPASVSEAFSAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQIDYDFVGKLETLDEDAAQLLRFLKVDSQLHFPPSYRNRTASSWEEDWFANIPLAWRQQLYKLYEADFVLFGYPKPENLLRD

Target ORF information:

RefSeq Version	XM_006504725.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbohydrate sulfotransferase 12 (Chst12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504725.2

ORF Insert Sequence:

ATGACCAAGC CGCGGCTCTT CCGGCTGTGG CTGGTACTAG GGTCGGCTCT CATGATCCTT 
TTGATCATTG TATATTGGGA CAACGTGGGC ACCGCCCACT TCTATCTGCA CACGTCTCTC 
TCCAGGCCAC ACATCCTAGA ACCCCTTCCC ACCCAGGGAT TGGTGGAGGA GAACGTGTTC 
ACATCCGACG TGGATGAGTT TTTGGATACT CTCCTTAGTT CTGACGCAAA GCACAACGAC 
CTTTCCAGGA GAAAAACTGA GCAGCCCCCG GTGCCCGCCC CCAGCAAGCC AGTCTTGAGC 
CACATGGAGG AGAACGTGAG AGGCTACGAC TGGTCCACTC ATGATGCCCA TCAGAACCCT 
GACCGGGACA GGCAGCAGGC CGAGAGGAGG AGCCTGCTGA GAGACTTCTG TGCCAACGCC 
AGCCTGGCAT TCCCCACCAA GGACCGCTCT TTTGACGACA TCCCCAACTA CGAACTGAAC 
CACCTGATCG TGGACGACCG CCACGGGGTC ATCTACTGCT ACGTGCCCAA GGTGGCCTGC 
ACCAACTGGA AGCGAGTGAT GATCGTGCTG AGCGAGAGCC TGCTGGACCG GGGCAGCCCC 
TACCGAGACC CCCTGGACAT CCCCCGGGAA CACGTGCACA ACACCAGCAC GCACCTCACC 
TTCAACAAGT TCTGGCGCCG CTACGGAAAG TTCTCCCGTC ACCTAATGAA GGTGAAGCTG 
AAGAAGTACA CCAAGTTCCT GTTCGTGCGC GACCCCTTTG TGCGCCTCAT CTCAGCCTTC 
CGCAGCAAGT TCGAGCTGGA GAACGAAGAG TTTTACCGCA AGTTCGCGGT GCCCATGCTC 
CGACTGTACG CCAACCACAC CAGCCTGCCC GCCTCGGTGA GTGAGGCTTT CAGCGCCGGG 
CTCAAGGTCT CCTTCGCCAA CTTCATCCAG TACCTCCTGG ACCCACACAC GGAGAAGCTG 
GCGCCTTTCA ACGAGCACTG GCGACAGGTG TACCGCCTCT GCCACCCGTG CCAGATAGAC 
TATGACTTCG TGGGGAAGCT GGAGACGCTC GATGAGGACG CTGCCCAGCT CCTGAGGTTC 
CTCAAGGTAG ACTCCCAGCT CCACTTCCCC CCCAGTTATC GGAACAGGAC GGCCAGCAGC 
TGGGAGGAAG ACTGGTTTGC CAACATCCCC CTGGCATGGA GGCAACAGCT CTATAAACTC 
TACGAGGCCG ACTTTGTTCT CTTTGGCTAC CCCAAGCCAG AAAACCTGCT CAGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chst12 ( XM_006504725.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504788.1 Mus musculus carbohydrate sulfotransferase 12 (Chst12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12449
Clone ID Related Accession (Same CDS sequence)	XM_006504725.2 , NM_021528.3 , XM_006504725.3
Accession Version	XM_006504725.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	carbohydrate sulfotransferase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC CGCGGCTCTT CCGGCTGTGG CTGGTACTAG GGTCGGCTCT CATGATCCTT 
TTGATCATTG TATATTGGGA CAACGTGGGC ACCGCCCACT TCTATCTGCA CACGTCTCTC 
TCCAGGCCAC ACATCCTAGA ACCCCTTCCC ACCCAGGGAT TGGTGGAGGA GAACGTGTTC 
ACATCCGACG TGGATGAGTT TTTGGATACT CTCCTTAGTT CTGACGCAAA GCACAACGAC 
CTTTCCAGGA GAAAAACTGA GCAGCCCCCG GTGCCCGCCC CCAGCAAGCC AGTCTTGAGC 
CACATGGAGG AGAACGTGAG AGGCTACGAC TGGTCCACTC ATGATGCCCA TCAGAACCCT 
GACCGGGACA GGCAGCAGGC CGAGAGGAGG AGCCTGCTGA GAGACTTCTG TGCCAACGCC 
AGCCTGGCAT TCCCCACCAA GGACCGCTCT TTTGACGACA TCCCCAACTA CGAACTGAAC 
CACCTGATCG TGGACGACCG CCACGGGGTC ATCTACTGCT ACGTGCCCAA GGTGGCCTGC 
ACCAACTGGA AGCGAGTGAT GATCGTGCTG AGCGAGAGCC TGCTGGACCG GGGCAGCCCC 
TACCGAGACC CCCTGGACAT CCCCCGGGAA CACGTGCACA ACACCAGCAC GCACCTCACC 
TTCAACAAGT TCTGGCGCCG CTACGGAAAG TTCTCCCGTC ACCTAATGAA GGTGAAGCTG 
AAGAAGTACA CCAAGTTCCT GTTCGTGCGC GACCCCTTTG TGCGCCTCAT CTCAGCCTTC 
CGCAGCAAGT TCGAGCTGGA GAACGAAGAG TTTTACCGCA AGTTCGCGGT GCCCATGCTC 
CGACTGTACG CCAACCACAC CAGCCTGCCC GCCTCGGTGA GTGAGGCTTT CAGCGCCGGG 
CTCAAGGTCT CCTTCGCCAA CTTCATCCAG TACCTCCTGG ACCCACACAC GGAGAAGCTG 
GCGCCTTTCA ACGAGCACTG GCGACAGGTG TACCGCCTCT GCCACCCGTG CCAGATAGAC 
TATGACTTCG TGGGGAAGCT GGAGACGCTC GATGAGGACG CTGCCCAGCT CCTGAGGTTC 
CTCAAGGTAG ACTCCCAGCT CCACTTCCCC CCCAGTTATC GGAACAGGAC GGCCAGCAGC 
TGGGAGGAAG ACTGGTTTGC CAACATCCCC CTGGCATGGA GGCAACAGCT CTATAAACTC 
TACGAGGCCG ACTTTGTTCT CTTTGGCTAC CCCAAGCCAG AAAACCTGCT CAGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504788.1
CDS	239..1498
Translation	MTKPRLFRLWLVLGSALMILLIIVYWDNVGTAHFYLHTSLSRPHILEPLPTQGLVEENVFTSDVDEFLDTLLSSDAKHNDLSRRKTEQPPVPAPSKPVLSHMEENVRGYDWSTHDAHQNPDRDRQQAERRSLLRDFCANASLAFPTKDRSFDDIPNYELNHLIVDDRHGVIYCYVPKVACTNWKRVMIVLSESLLDRGSPYRDPLDIPREHVHNTSTHLTFNKFWRRYGKFSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLRLYANHTSLPASVSEAFSAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQIDYDFVGKLETLDEDAAQLLRFLKVDSQLHFPPSYRNRTASSWEEDWFANIPLAWRQQLYKLYEADFVLFGYPKPENLLRD

Target ORF information:

RefSeq Version	XM_006504725.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbohydrate sulfotransferase 12 (Chst12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504725.3

ORF Insert Sequence:

ATGACCAAGC CGCGGCTCTT CCGGCTGTGG CTGGTACTAG GGTCGGCTCT CATGATCCTT 
TTGATCATTG TATATTGGGA CAACGTGGGC ACCGCCCACT TCTATCTGCA CACGTCTCTC 
TCCAGGCCAC ACATCCTAGA ACCCCTTCCC ACCCAGGGAT TGGTGGAGGA GAACGTGTTC 
ACATCCGACG TGGATGAGTT TTTGGATACT CTCCTTAGTT CTGACGCAAA GCACAACGAC 
CTTTCCAGGA GAAAAACTGA GCAGCCCCCG GTGCCCGCCC CCAGCAAGCC AGTCTTGAGC 
CACATGGAGG AGAACGTGAG AGGCTACGAC TGGTCCACTC ATGATGCCCA TCAGAACCCT 
GACCGGGACA GGCAGCAGGC CGAGAGGAGG AGCCTGCTGA GAGACTTCTG TGCCAACGCC 
AGCCTGGCAT TCCCCACCAA GGACCGCTCT TTTGACGACA TCCCCAACTA CGAACTGAAC 
CACCTGATCG TGGACGACCG CCACGGGGTC ATCTACTGCT ACGTGCCCAA GGTGGCCTGC 
ACCAACTGGA AGCGAGTGAT GATCGTGCTG AGCGAGAGCC TGCTGGACCG GGGCAGCCCC 
TACCGAGACC CCCTGGACAT CCCCCGGGAA CACGTGCACA ACACCAGCAC GCACCTCACC 
TTCAACAAGT TCTGGCGCCG CTACGGAAAG TTCTCCCGTC ACCTAATGAA GGTGAAGCTG 
AAGAAGTACA CCAAGTTCCT GTTCGTGCGC GACCCCTTTG TGCGCCTCAT CTCAGCCTTC 
CGCAGCAAGT TCGAGCTGGA GAACGAAGAG TTTTACCGCA AGTTCGCGGT GCCCATGCTC 
CGACTGTACG CCAACCACAC CAGCCTGCCC GCCTCGGTGA GTGAGGCTTT CAGCGCCGGG 
CTCAAGGTCT CCTTCGCCAA CTTCATCCAG TACCTCCTGG ACCCACACAC GGAGAAGCTG 
GCGCCTTTCA ACGAGCACTG GCGACAGGTG TACCGCCTCT GCCACCCGTG CCAGATAGAC 
TATGACTTCG TGGGGAAGCT GGAGACGCTC GATGAGGACG CTGCCCAGCT CCTGAGGTTC 
CTCAAGGTAG ACTCCCAGCT CCACTTCCCC CCCAGTTATC GGAACAGGAC GGCCAGCAGC 
TGGGAGGAAG ACTGGTTTGC CAACATCCCC CTGGCATGGA GGCAACAGCT CTATAAACTC 
TACGAGGCCG ACTTTGTTCT CTTTGGCTAC CCCAAGCCAG AAAACCTGCT CAGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.
Mammalian genome : official journal of the International Mammalian Genome Society24(5-6)228-39(2013 Jun)
Nakahara M,Tateyama H,Araki M,Nakagata N,Yamamura K,Araki K

Chondroitin 4-O-sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1.
The Biochemical journal441(2)697-705(2012 Jan)
Izumikawa T,Koike T,Kitagawa H

Expression of multiple chondroitin/dermatan sulfotransferases in the neurogenic regions of the embryonic and adult central nervous system implies that complex chondroitin sulfates have a role in neural stem cell maintenance.
Stem cells (Dayton, Ohio)26(3)798-809(2008 Mar)
Akita K,von Holst A,Furukawa Y,Mikami T,Sugahara K,Faissner A

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

Exchangeable gene trap using the Cre/mutated lox system.
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)
Araki K,Imaizumi T,Sekimoto T,Yoshinobu K,Yoshimuta J,Akizuki M,Miura K,Araki M,Yamamura K

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Chst12 cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

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ORF Insert Sequence:

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