Mkks cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mkks
Entrez Gene ID	59030
Full Name	McKusick-Kaufman syndrome
Synonyms	1300013E18Rik,AI463362,AI957237,Bbs6
General protein information	Preferred Names McKusick-Kaufman syndrome Names McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin McKusick-Kaufman syndrome protein McKusick-Kaufman syndrome protein; MKKS protein protein Bbs6 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 F3
Summary	This gene encodes a protein which shares sequence similarity with other members of the type II chaperonin family. The encoded protein is a centrosome-shuttling protein and plays an important role in cytokinesis. This protein also interacts with other type II chaperonin members to form a complex known as the BBSome, which involves ciliary membrane biogenesis. This protein is encoded by a downstream open reading frame (dORF). Several upstream open reading frames (uORFs) have been identified, which repress the translation of the dORF, and two of which can encode small mitochondrial membrane proteins. Alternatively spliced transcripts encoding distinct isoforms have been found for this gene. [provided by RefSeq, Nov 2013].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499970.1	XP_006500033.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X1
NM_001286981.1	NP_001273910.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform 2
NM_001141946.1	NP_001135418.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform 1
NM_001286983.1	NP_001273912.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform 3
XM_011239721.1	XP_011238023.1	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X1
NM_021527.2	NP_067502.2	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MKKS	XP_001169410.1
Canis lupus familiaris (dog)	MKKS	XP_542894.1
Rattus norvegicus (Norway rat)	Mkks	NP_001008354.1
Homo sapiens (human)	MKKS	NP_740754.1
Mus musculus (house mouse)	Mkks	NP_067502.2
Danio rerio (zebrafish)	mkks	NP_956459.1
Xenopus tropicalis (tropical clawed frog)	mkks	XP_002931783.1
	MKKS	XP_001116196.1
Bos taurus (cattle)	MKKS	NP_001193100.1
Gallus gallus (chicken)	MKKS	NP_001186339.1

Gene Ontology
Bibliography

Related articles in PubMed

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Burnicka-Turek O, Steimle JD, Huang W, Felker L, Kamp A, Kweon J, Peterson M, Reeves RH, Maslen CL, Gruber PJ, Yang XH, Shendure J, Moskowitz IP
Human molecular genetics25(14)3011-3028(2016 07)

Regulation of Insulin Receptor Trafficking by Bardet Biedl Syndrome Proteins.
Starks RD, Beyer AM, Guo DF, Boland L, Zhang Q, Sheffield VC, Rahmouni K
PLoS genetics11(6)e1005311(2015 Jun)

Bardet-Biedl syndrome proteins control the cilia length through regulation of actin polymerization.
Hernandez-Hernandez V, Pravincumar P, Diaz-Font A, May-Simera H, Jenkins D, Knight M, Beales PL
Human molecular genetics22(19)3858-68(2013 Oct)

Translational repression of the McKusick-Kaufman syndrome transcript by unique upstream open reading frames encoding mitochondrial proteins with alternative polyadenylation sites.
Akimoto C, Sakashita E, Kasashima K, Kuroiwa K, Tominaga K, Hamamoto T, Endo H
Biochimica et biophysica acta1830(3)2728-38(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Three uORFs (uMKKS0, uMKKS1 and uMKKS2) are reported, and they can repress the translation of the downstream MKKS ORF. uMKKS1 and uMKKS2 are highly conserved in mammals and they encode two different mitochondrial membrane proteins respectively.
Title: Translational repression of the McKusick-Kaufman syndrome transcript by unique upstream open reading frames encoding mitochondrial proteins with alternative polyadenylation sites.

Combinations of Cep290rd16 & Mkksko alleles improved ciliogenesis & sensory functions vs either mutant alone. Altered association of CEP290 & MKKS affects multiprotein complex integrity at the cilia transition zone & basal body.
Title: Combining Cep290 and Mkks ciliopathy alleles in mice rescues sensory defects and restores ciliogenesis.

Data implicate Bardet-Biedl syndrome genes in the regulation of vascular function and demonstrate that disrupting Bbs2 and Bbs6 genes affect differentially the vascular function.
Title: Contrasting vascular effects caused by loss of Bardet-Biedl syndrome genes.

Bardet-Biedl syndrome (BBS) proteins mediate LepR trafficking and that impaired LepR signaling underlies energy imbalance in BBS.
Title: Requirement of Bardet-Biedl syndrome proteins for leptin receptor signaling.

Although BBS proteins were not required for ciliogenesis, their loss caused structural defects in a fraction of cilia covering mouse airway epithelia in Bbs1, Bbs2, Bbs4, and Bbs6 mutant mice.
Title: Loss of Bardet-Biedl syndrome proteins alters the morphology and function of motile cilia in airway epithelia.

The following Mkks gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mkks cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20052	NM_001141946.1 Latest version!	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20052	NM_021527.2 Latest version!	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20084	NM_001286981.1 Latest version!	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20101	NM_001286983.1 Latest version!	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu20052	XM_006499970.1 Latest version!	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20052	XM_011239721.1 Latest version!	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mkks ( NM_001141946.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001135418.1 Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20052
Clone ID Related Accession (Same CDS sequence)	XM_006499970.1 , NM_001141946.1 , XM_011239721.1 , NM_021527.2
Accession Version	NM_001141946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY735964.1 and BC080765.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC080765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 23671934 ##RefSeq-Attributes-END##

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGGCAAG TGGCCTTAGG 
GTGGCACTCT TCTGTACATC TTTATCTGGA GATTTTTCTA ATGCTGGAGA AGGAGTGGTG 
GTGGCCCATT ACCAAGTGTC CCTTGAGAAT GCAGTTTTAG AGCAGTTGCT TAATCTAGGA 
AGGCGACTAG TCACTGATCA TGTAGACCTT GTCCTGTGCC AAAAAGTAAT CCACCCATCT 
TTGAAACAGT TCTTCAGTGA GCGCCACGTT ATGGCCATCG ACAGAGTTGG GGTGACTCTG 
ATGGAATCTC TAAGCAAAGT GACAGGAGCA ACGCCTATTG GTTCTCTAAA CCCAATAGTT 
TCTACTACTT ATGGAAGTGT GAAAGATGTG TGCTCTGCAA GATTTGGCTC CAAACATTTT 
TTTCATCTTC TTCCTAATGA GGCCACTGTC TGCACCTTGC TTCTCTGCAG CAGAAATGAC 
ACCGCCTGGG AGGAGCTGAA GCTCACATGT CAAACAGCAA TGCACGTCTT GCAGTTAACA 
ATCAAGGAAC CGTGGGTTTT ATTGGGAGGT GGCTGTACAG AAACACACTT GGCTGCATAT 
GTCAGACACA AGGTTCATCA CGAGGCAGAA GCTATTGTCA GAGATGATGG GTGTACTCAG 
GCAAAGCTGC ATGTTGCTGC TGAAGCATTT TGCAGTGCTC TGGAGTCCGT TGCTGGCTCT 
TTGGAACATG ATGGTGGTGA AATCCTCATT GACACGAAGT ATGGACACCT TTGGTCCTGT 
CAAGCAGATT CTGCCTCTGT TGGTAACTGG TCAGATACGC TGTCACGGTG TGGCTGTGGT 
TTGTACAACA GCCAGGAAGA GCTCAGCTGG TCTGTCTTAA GAAGTACTTA TCATCCTTTT 
GCACCACAAA CCTGCCTTCC ACAGGCAGCT TTGGGCTCAG CCAGTAACCT GACTGTGGAC 
TGCTTCACTG CCAAGCTGAG TGGCTTACAG GTGGCTGTAG AGACAGCCAA TTTGATTTTA 
GATCTTTCAT ATGTCATTGA AGATAAAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135418.1
CDS	761..2473
Translation	MSRLEAKKPSLCKTEPLTSEKVRSTLSVLKGVIASCYGPSGRLKQLHNGLGGCVYTTSQSSALLRNLSVTHPVLKILTSSVQNHVSCFSDCGLFTAILCCNLIENIQRLDLTPATAIKLNKYLLSLCTSYLKSEACSCRIPVDFRSTHTFLSLVHSILTSKPACMLTRKETDHIGALILKAFLLTIPESTEERMVLGKSIIVPLKGQRVTDSTVLPGLLIEASEVQLRRLLPTQKASGLRVALFCTSLSGDFSNAGEGVVVAHYQVSLENAVLEQLLNLGRRLVTDHVDLVLCQKVIHPSLKQFFSERHVMAIDRVGVTLMESLSKVTGATPIGSLNPIVSTTYGSVKDVCSARFGSKHFFHLLPNEATVCTLLLCSRNDTAWEELKLTCQTAMHVLQLTIKEPWVLLGGGCTETHLAAYVRHKVHHEAEAIVRDDGCTQAKLHVAAEAFCSALESVAGSLEHDGGEILIDTKYGHLWSCQADSASVGNWSDTLSRCGCGLYNSQEELSWSVLRSTYHPFAPQTCLPQAALGSASNLTVDCFTAKLSGLQVAVETANLILDLSYVIEDKN

Target ORF information:

RefSeq Version	NM_001141946.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001141946.1

ORF Insert Sequence:

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGGCAAG TGGCCTTAGG 
GTGGCACTCT TCTGTACATC TTTATCTGGA GATTTTTCTA ATGCTGGAGA AGGAGTGGTG 
GTGGCCCATT ACCAAGTGTC CCTTGAGAAT GCAGTTTTAG AGCAGTTGCT TAATCTAGGA 
AGGCGACTAG TCACTGATCA TGTAGACCTT GTCCTGTGCC AAAAAGTAAT CCACCCATCT 
TTGAAACAGT TCTTCAGTGA GCGCCACGTT ATGGCCATCG ACAGAGTTGG GGTGACTCTG 
ATGGAATCTC TAAGCAAAGT GACAGGAGCA ACGCCTATTG GTTCTCTAAA CCCAATAGTT 
TCTACTACTT ATGGAAGTGT GAAAGATGTG TGCTCTGCAA GATTTGGCTC CAAACATTTT 
TTTCATCTTC TTCCTAATGA GGCCACTGTC TGCACCTTGC TTCTCTGCAG CAGAAATGAC 
ACCGCCTGGG AGGAGCTGAA GCTCACATGT CAAACAGCAA TGCACGTCTT GCAGTTAACA 
ATCAAGGAAC CGTGGGTTTT ATTGGGAGGT GGCTGTACAG AAACACACTT GGCTGCATAT 
GTCAGACACA AGGTTCATCA CGAGGCAGAA GCTATTGTCA GAGATGATGG GTGTACTCAG 
GCAAAGCTGC ATGTTGCTGC TGAAGCATTT TGCAGTGCTC TGGAGTCCGT TGCTGGCTCT 
TTGGAACATG ATGGTGGTGA AATCCTCATT GACACGAAGT ATGGACACCT TTGGTCCTGT 
CAAGCAGATT CTGCCTCTGT TGGTAACTGG TCAGATACGC TGTCACGGTG TGGCTGTGGT 
TTGTACAACA GCCAGGAAGA GCTCAGCTGG TCTGTCTTAA GAAGTACTTA TCATCCTTTT 
GCACCACAAA CCTGCCTTCC ACAGGCAGCT TTGGGCTCAG CCAGTAACCT GACTGTGGAC 
TGCTTCACTG CCAAGCTGAG TGGCTTACAG GTGGCTGTAG AGACAGCCAA TTTGATTTTA 
GATCTTTCAT ATGTCATTGA AGATAAAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkks ( NM_021527.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067502.2 Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20052
Clone ID Related Accession (Same CDS sequence)	XM_006499970.1 , NM_001141946.1 , XM_011239721.1 , NM_021527.2
Accession Version	NM_021527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY735964.1, CJ157733.1 and BC080765.1. On Nov 21, 2008 this sequence version replaced NM_021527.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.172095.1, AF254074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 23671934 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGGCAAG TGGCCTTAGG 
GTGGCACTCT TCTGTACATC TTTATCTGGA GATTTTTCTA ATGCTGGAGA AGGAGTGGTG 
GTGGCCCATT ACCAAGTGTC CCTTGAGAAT GCAGTTTTAG AGCAGTTGCT TAATCTAGGA 
AGGCGACTAG TCACTGATCA TGTAGACCTT GTCCTGTGCC AAAAAGTAAT CCACCCATCT 
TTGAAACAGT TCTTCAGTGA GCGCCACGTT ATGGCCATCG ACAGAGTTGG GGTGACTCTG 
ATGGAATCTC TAAGCAAAGT GACAGGAGCA ACGCCTATTG GTTCTCTAAA CCCAATAGTT 
TCTACTACTT ATGGAAGTGT GAAAGATGTG TGCTCTGCAA GATTTGGCTC CAAACATTTT 
TTTCATCTTC TTCCTAATGA GGCCACTGTC TGCACCTTGC TTCTCTGCAG CAGAAATGAC 
ACCGCCTGGG AGGAGCTGAA GCTCACATGT CAAACAGCAA TGCACGTCTT GCAGTTAACA 
ATCAAGGAAC CGTGGGTTTT ATTGGGAGGT GGCTGTACAG AAACACACTT GGCTGCATAT 
GTCAGACACA AGGTTCATCA CGAGGCAGAA GCTATTGTCA GAGATGATGG GTGTACTCAG 
GCAAAGCTGC ATGTTGCTGC TGAAGCATTT TGCAGTGCTC TGGAGTCCGT TGCTGGCTCT 
TTGGAACATG ATGGTGGTGA AATCCTCATT GACACGAAGT ATGGACACCT TTGGTCCTGT 
CAAGCAGATT CTGCCTCTGT TGGTAACTGG TCAGATACGC TGTCACGGTG TGGCTGTGGT 
TTGTACAACA GCCAGGAAGA GCTCAGCTGG TCTGTCTTAA GAAGTACTTA TCATCCTTTT 
GCACCACAAA CCTGCCTTCC ACAGGCAGCT TTGGGCTCAG CCAGTAACCT GACTGTGGAC 
TGCTTCACTG CCAAGCTGAG TGGCTTACAG GTGGCTGTAG AGACAGCCAA TTTGATTTTA 
GATCTTTCAT ATGTCATTGA AGATAAAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067502.2
CDS	997..2709
Translation	MSRLEAKKPSLCKTEPLTSEKVRSTLSVLKGVIASCYGPSGRLKQLHNGLGGCVYTTSQSSALLRNLSVTHPVLKILTSSVQNHVSCFSDCGLFTAILCCNLIENIQRLDLTPATAIKLNKYLLSLCTSYLKSEACSCRIPVDFRSTHTFLSLVHSILTSKPACMLTRKETDHIGALILKAFLLTIPESTEERMVLGKSIIVPLKGQRVTDSTVLPGLLIEASEVQLRRLLPTQKASGLRVALFCTSLSGDFSNAGEGVVVAHYQVSLENAVLEQLLNLGRRLVTDHVDLVLCQKVIHPSLKQFFSERHVMAIDRVGVTLMESLSKVTGATPIGSLNPIVSTTYGSVKDVCSARFGSKHFFHLLPNEATVCTLLLCSRNDTAWEELKLTCQTAMHVLQLTIKEPWVLLGGGCTETHLAAYVRHKVHHEAEAIVRDDGCTQAKLHVAAEAFCSALESVAGSLEHDGGEILIDTKYGHLWSCQADSASVGNWSDTLSRCGCGLYNSQEELSWSVLRSTYHPFAPQTCLPQAALGSASNLTVDCFTAKLSGLQVAVETANLILDLSYVIEDKN

Target ORF information:

RefSeq Version	NM_021527.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021527.2

ORF Insert Sequence:

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGGCAAG TGGCCTTAGG 
GTGGCACTCT TCTGTACATC TTTATCTGGA GATTTTTCTA ATGCTGGAGA AGGAGTGGTG 
GTGGCCCATT ACCAAGTGTC CCTTGAGAAT GCAGTTTTAG AGCAGTTGCT TAATCTAGGA 
AGGCGACTAG TCACTGATCA TGTAGACCTT GTCCTGTGCC AAAAAGTAAT CCACCCATCT 
TTGAAACAGT TCTTCAGTGA GCGCCACGTT ATGGCCATCG ACAGAGTTGG GGTGACTCTG 
ATGGAATCTC TAAGCAAAGT GACAGGAGCA ACGCCTATTG GTTCTCTAAA CCCAATAGTT 
TCTACTACTT ATGGAAGTGT GAAAGATGTG TGCTCTGCAA GATTTGGCTC CAAACATTTT 
TTTCATCTTC TTCCTAATGA GGCCACTGTC TGCACCTTGC TTCTCTGCAG CAGAAATGAC 
ACCGCCTGGG AGGAGCTGAA GCTCACATGT CAAACAGCAA TGCACGTCTT GCAGTTAACA 
ATCAAGGAAC CGTGGGTTTT ATTGGGAGGT GGCTGTACAG AAACACACTT GGCTGCATAT 
GTCAGACACA AGGTTCATCA CGAGGCAGAA GCTATTGTCA GAGATGATGG GTGTACTCAG 
GCAAAGCTGC ATGTTGCTGC TGAAGCATTT TGCAGTGCTC TGGAGTCCGT TGCTGGCTCT 
TTGGAACATG ATGGTGGTGA AATCCTCATT GACACGAAGT ATGGACACCT TTGGTCCTGT 
CAAGCAGATT CTGCCTCTGT TGGTAACTGG TCAGATACGC TGTCACGGTG TGGCTGTGGT 
TTGTACAACA GCCAGGAAGA GCTCAGCTGG TCTGTCTTAA GAAGTACTTA TCATCCTTTT 
GCACCACAAA CCTGCCTTCC ACAGGCAGCT TTGGGCTCAG CCAGTAACCT GACTGTGGAC 
TGCTTCACTG CCAAGCTGAG TGGCTTACAG GTGGCTGTAG AGACAGCCAA TTTGATTTTA 
GATCTTTCAT ATGTCATTGA AGATAAAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkks ( NM_001286981.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273910.1 Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu20084
Clone ID Related Accession (Same CDS sequence)	NM_001286981.1
Accession Version	NM_001286981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY735964.1, CJ157733.1, AK008406.1, AK004987.1 and AI662260.1. Transcript Variant: This variant (3) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK004987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 23671934 ##RefSeq-Attributes-END##

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGTTCTT CAGTGAGCGC 
CACGTTATGG CCATCGACAG AGTTGGGGTG ACTCTGATGG AATCTCTAAG CAAAGTGACA 
GGAGCAACGC CTATTGGTTC TCTAAACCCA ATAGTTTCTA CTACTTATGG AAGTGTGAAA 
GATGTGTGCT CTGCAAGATT TGGCTCCAAA CATTTTTTTC ATCTTCTTCC TAATGAGGCC 
ACTGTCTGCA CCTTGCTTCT CTGCAGCAGA AATGACACCG CCTGGGAGGA GCTGAAGCTC 
ACATGTCAAA CAGCAATGCA CGTCTTGCAG TTAACAATCA AGGAACCGTG GGTTTTATTG 
GGAGGTGGCT GTACAGAAAC ACACTTGGCT GCATATGTCA GACACAAGGT TCATCACGAG 
GCAGAAGCTA TTGTCAGAGA TGATGGGTGT ACTCAGGCAA AGCTGCATGT TGCTGCTGAA 
GCATTTTGCA GTGCTCTGGA GTCCGTTGCT GGCTCTTTGG AACATGATGG TGGTGAAATC 
CTCATTGACA CGAAGTATGG ACACCTTTGG TCCTGTCAAG CAGATTCTGC CTCTGTTGGT 
AACTGGTCAG ATACGCTGTC ACGGTGTGGC TGTGGTTTGT ACAACAGCCA GGAAGAGCTC 
AGCTGGTCTG TCTTAAGAAG TACTTATCAT CCTTTTGCAC CACAAACCTG CCTTCCACAG 
GCAGCTTTGG GCTCAGCCAG TAACCTGACT GTGGACTGCT TCACTGCCAA GCTGAGTGGC 
TTACAGGTGG CTGTAGAGAC AGCCAATTTG ATTTTAGATC TTTCATATGT CATTGAAGAT 
AAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273910.1
CDS	997..2505
Translation	MSRLEAKKPSLCKTEPLTSEKVRSTLSVLKGVIASCYGPSGRLKQLHNGLGGCVYTTSQSSALLRNLSVTHPVLKILTSSVQNHVSCFSDCGLFTAILCCNLIENIQRLDLTPATAIKLNKYLLSLCTSYLKSEACSCRIPVDFRSTHTFLSLVHSILTSKPACMLTRKETDHIGALILKAFLLTIPESTEERMVLGKSIIVPLKGQRVTDSTVLPGLLIEASEVQLRRLLPTQKFFSERHVMAIDRVGVTLMESLSKVTGATPIGSLNPIVSTTYGSVKDVCSARFGSKHFFHLLPNEATVCTLLLCSRNDTAWEELKLTCQTAMHVLQLTIKEPWVLLGGGCTETHLAAYVRHKVHHEAEAIVRDDGCTQAKLHVAAEAFCSALESVAGSLEHDGGEILIDTKYGHLWSCQADSASVGNWSDTLSRCGCGLYNSQEELSWSVLRSTYHPFAPQTCLPQAALGSASNLTVDCFTAKLSGLQVAVETANLILDLSYVIEDKN

Target ORF information:

RefSeq Version	NM_001286981.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286981.1

ORF Insert Sequence:

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGTTCTT CAGTGAGCGC 
CACGTTATGG CCATCGACAG AGTTGGGGTG ACTCTGATGG AATCTCTAAG CAAAGTGACA 
GGAGCAACGC CTATTGGTTC TCTAAACCCA ATAGTTTCTA CTACTTATGG AAGTGTGAAA 
GATGTGTGCT CTGCAAGATT TGGCTCCAAA CATTTTTTTC ATCTTCTTCC TAATGAGGCC 
ACTGTCTGCA CCTTGCTTCT CTGCAGCAGA AATGACACCG CCTGGGAGGA GCTGAAGCTC 
ACATGTCAAA CAGCAATGCA CGTCTTGCAG TTAACAATCA AGGAACCGTG GGTTTTATTG 
GGAGGTGGCT GTACAGAAAC ACACTTGGCT GCATATGTCA GACACAAGGT TCATCACGAG 
GCAGAAGCTA TTGTCAGAGA TGATGGGTGT ACTCAGGCAA AGCTGCATGT TGCTGCTGAA 
GCATTTTGCA GTGCTCTGGA GTCCGTTGCT GGCTCTTTGG AACATGATGG TGGTGAAATC 
CTCATTGACA CGAAGTATGG ACACCTTTGG TCCTGTCAAG CAGATTCTGC CTCTGTTGGT 
AACTGGTCAG ATACGCTGTC ACGGTGTGGC TGTGGTTTGT ACAACAGCCA GGAAGAGCTC 
AGCTGGTCTG TCTTAAGAAG TACTTATCAT CCTTTTGCAC CACAAACCTG CCTTCCACAG 
GCAGCTTTGG GCTCAGCCAG TAACCTGACT GTGGACTGCT TCACTGCCAA GCTGAGTGGC 
TTACAGGTGG CTGTAGAGAC AGCCAATTTG ATTTTAGATC TTTCATATGT CATTGAAGAT 
AAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkks ( NM_001286983.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273912.1 Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu20101
Clone ID Related Accession (Same CDS sequence)	NM_001286983.1
Accession Version	NM_001286983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY735964.1, CJ157733.1 and BC100336.1. Transcript Variant: This variant (4) lacks an internal exon, which results in a downstream start codon compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC100336.1, CK792412.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCACGTCT TGCAGTTAAC AATCAAGGAA CCGTGGGTTT TATTGGGAGG TGGCTGTACA 
GAAACACACT TGGCTGCATA TGTCAGACAC AAGGTTCATC ACGAGGCAGA AGCTATTGTC 
AGAGATGATG GGTGTACTCA GGCAAAGCTG CATGTTGCTG CTGAAGCATT TTGCAGTGCT 
CTGGAGTCCG TTGCTGGCTC TTTGGAACAT GATGGTGGTG AAATCCTCAT TGACACGAAG 
TATGGACACC TTTGGTCCTG TCAAGCAGAT TCTGCCTCTG TTGGTAACTG GTCAGATACG 
CTGTCACGGT GTGGCTGTGG TTTGTACAAC AGCCAGGAAG AGCTCAGCTG GTCTGTCTTA 
AGAAGTACTT ATCATCCTTT TGCACCACAA ACCTGCCTTC CACAGGCAGC TTTGGGCTCA 
GCCAGTAACC TGACTGTGGA CTGCTTCACT GCCAAGCTGA GTGGCTTACA GGTGGCTGTA 
GAGACAGCCA ATTTGATTTT AGATCTTTCA TATGTCATTG AAGATAAAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273912.1
CDS	787..1320
Translation	MHVLQLTIKEPWVLLGGGCTETHLAAYVRHKVHHEAEAIVRDDGCTQAKLHVAAEAFCSALESVAGSLEHDGGEILIDTKYGHLWSCQADSASVGNWSDTLSRCGCGLYNSQEELSWSVLRSTYHPFAPQTCLPQAALGSASNLTVDCFTAKLSGLQVAVETANLILDLSYVIEDKN

Target ORF information:

RefSeq Version	NM_001286983.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286983.1

ORF Insert Sequence:

ATGCACGTCT TGCAGTTAAC AATCAAGGAA CCGTGGGTTT TATTGGGAGG TGGCTGTACA 
GAAACACACT TGGCTGCATA TGTCAGACAC AAGGTTCATC ACGAGGCAGA AGCTATTGTC 
AGAGATGATG GGTGTACTCA GGCAAAGCTG CATGTTGCTG CTGAAGCATT TTGCAGTGCT 
CTGGAGTCCG TTGCTGGCTC TTTGGAACAT GATGGTGGTG AAATCCTCAT TGACACGAAG 
TATGGACACC TTTGGTCCTG TCAAGCAGAT TCTGCCTCTG TTGGTAACTG GTCAGATACG 
CTGTCACGGT GTGGCTGTGG TTTGTACAAC AGCCAGGAAG AGCTCAGCTG GTCTGTCTTA 
AGAAGTACTT ATCATCCTTT TGCACCACAA ACCTGCCTTC CACAGGCAGC TTTGGGCTCA 
GCCAGTAACC TGACTGTGGA CTGCTTCACT GCCAAGCTGA GTGGCTTACA GGTGGCTGTA 
GAGACAGCCA ATTTGATTTT AGATCTTTCA TATGTCATTG AAGATAAAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkks ( XM_006499970.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500033.1 Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant X2, mRNA.

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CloneID	OMu20052
Clone ID Related Accession (Same CDS sequence)	XM_006499970.1 , NM_001141946.1 , XM_011239721.1 , NM_021527.2
Accession Version	XM_006499970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGGCAAG TGGCCTTAGG 
GTGGCACTCT TCTGTACATC TTTATCTGGA GATTTTTCTA ATGCTGGAGA AGGAGTGGTG 
GTGGCCCATT ACCAAGTGTC CCTTGAGAAT GCAGTTTTAG AGCAGTTGCT TAATCTAGGA 
AGGCGACTAG TCACTGATCA TGTAGACCTT GTCCTGTGCC AAAAAGTAAT CCACCCATCT 
TTGAAACAGT TCTTCAGTGA GCGCCACGTT ATGGCCATCG ACAGAGTTGG GGTGACTCTG 
ATGGAATCTC TAAGCAAAGT GACAGGAGCA ACGCCTATTG GTTCTCTAAA CCCAATAGTT 
TCTACTACTT ATGGAAGTGT GAAAGATGTG TGCTCTGCAA GATTTGGCTC CAAACATTTT 
TTTCATCTTC TTCCTAATGA GGCCACTGTC TGCACCTTGC TTCTCTGCAG CAGAAATGAC 
ACCGCCTGGG AGGAGCTGAA GCTCACATGT CAAACAGCAA TGCACGTCTT GCAGTTAACA 
ATCAAGGAAC CGTGGGTTTT ATTGGGAGGT GGCTGTACAG AAACACACTT GGCTGCATAT 
GTCAGACACA AGGTTCATCA CGAGGCAGAA GCTATTGTCA GAGATGATGG GTGTACTCAG 
GCAAAGCTGC ATGTTGCTGC TGAAGCATTT TGCAGTGCTC TGGAGTCCGT TGCTGGCTCT 
TTGGAACATG ATGGTGGTGA AATCCTCATT GACACGAAGT ATGGACACCT TTGGTCCTGT 
CAAGCAGATT CTGCCTCTGT TGGTAACTGG TCAGATACGC TGTCACGGTG TGGCTGTGGT 
TTGTACAACA GCCAGGAAGA GCTCAGCTGG TCTGTCTTAA GAAGTACTTA TCATCCTTTT 
GCACCACAAA CCTGCCTTCC ACAGGCAGCT TTGGGCTCAG CCAGTAACCT GACTGTGGAC 
TGCTTCACTG CCAAGCTGAG TGGCTTACAG GTGGCTGTAG AGACAGCCAA TTTGATTTTA 
GATCTTTCAT ATGTCATTGA AGATAAAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500033.1
CDS	721..2433
Translation	MSRLEAKKPSLCKTEPLTSEKVRSTLSVLKGVIASCYGPSGRLKQLHNGLGGCVYTTSQSSALLRNLSVTHPVLKILTSSVQNHVSCFSDCGLFTAILCCNLIENIQRLDLTPATAIKLNKYLLSLCTSYLKSEACSCRIPVDFRSTHTFLSLVHSILTSKPACMLTRKETDHIGALILKAFLLTIPESTEERMVLGKSIIVPLKGQRVTDSTVLPGLLIEASEVQLRRLLPTQKASGLRVALFCTSLSGDFSNAGEGVVVAHYQVSLENAVLEQLLNLGRRLVTDHVDLVLCQKVIHPSLKQFFSERHVMAIDRVGVTLMESLSKVTGATPIGSLNPIVSTTYGSVKDVCSARFGSKHFFHLLPNEATVCTLLLCSRNDTAWEELKLTCQTAMHVLQLTIKEPWVLLGGGCTETHLAAYVRHKVHHEAEAIVRDDGCTQAKLHVAAEAFCSALESVAGSLEHDGGEILIDTKYGHLWSCQADSASVGNWSDTLSRCGCGLYNSQEELSWSVLRSTYHPFAPQTCLPQAALGSASNLTVDCFTAKLSGLQVAVETANLILDLSYVIEDKN

Target ORF information:

RefSeq Version	XM_006499970.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499970.1

ORF Insert Sequence:

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGGCAAG TGGCCTTAGG 
GTGGCACTCT TCTGTACATC TTTATCTGGA GATTTTTCTA ATGCTGGAGA AGGAGTGGTG 
GTGGCCCATT ACCAAGTGTC CCTTGAGAAT GCAGTTTTAG AGCAGTTGCT TAATCTAGGA 
AGGCGACTAG TCACTGATCA TGTAGACCTT GTCCTGTGCC AAAAAGTAAT CCACCCATCT 
TTGAAACAGT TCTTCAGTGA GCGCCACGTT ATGGCCATCG ACAGAGTTGG GGTGACTCTG 
ATGGAATCTC TAAGCAAAGT GACAGGAGCA ACGCCTATTG GTTCTCTAAA CCCAATAGTT 
TCTACTACTT ATGGAAGTGT GAAAGATGTG TGCTCTGCAA GATTTGGCTC CAAACATTTT 
TTTCATCTTC TTCCTAATGA GGCCACTGTC TGCACCTTGC TTCTCTGCAG CAGAAATGAC 
ACCGCCTGGG AGGAGCTGAA GCTCACATGT CAAACAGCAA TGCACGTCTT GCAGTTAACA 
ATCAAGGAAC CGTGGGTTTT ATTGGGAGGT GGCTGTACAG AAACACACTT GGCTGCATAT 
GTCAGACACA AGGTTCATCA CGAGGCAGAA GCTATTGTCA GAGATGATGG GTGTACTCAG 
GCAAAGCTGC ATGTTGCTGC TGAAGCATTT TGCAGTGCTC TGGAGTCCGT TGCTGGCTCT 
TTGGAACATG ATGGTGGTGA AATCCTCATT GACACGAAGT ATGGACACCT TTGGTCCTGT 
CAAGCAGATT CTGCCTCTGT TGGTAACTGG TCAGATACGC TGTCACGGTG TGGCTGTGGT 
TTGTACAACA GCCAGGAAGA GCTCAGCTGG TCTGTCTTAA GAAGTACTTA TCATCCTTTT 
GCACCACAAA CCTGCCTTCC ACAGGCAGCT TTGGGCTCAG CCAGTAACCT GACTGTGGAC 
TGCTTCACTG CCAAGCTGAG TGGCTTACAG GTGGCTGTAG AGACAGCCAA TTTGATTTTA 
GATCTTTCAT ATGTCATTGA AGATAAAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkks ( XM_011239721.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238023.1 Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20052
Clone ID Related Accession (Same CDS sequence)	XM_006499970.1 , NM_001141946.1 , XM_011239721.1 , NM_021527.2
Accession Version	XM_011239721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGGCAAG TGGCCTTAGG 
GTGGCACTCT TCTGTACATC TTTATCTGGA GATTTTTCTA ATGCTGGAGA AGGAGTGGTG 
GTGGCCCATT ACCAAGTGTC CCTTGAGAAT GCAGTTTTAG AGCAGTTGCT TAATCTAGGA 
AGGCGACTAG TCACTGATCA TGTAGACCTT GTCCTGTGCC AAAAAGTAAT CCACCCATCT 
TTGAAACAGT TCTTCAGTGA GCGCCACGTT ATGGCCATCG ACAGAGTTGG GGTGACTCTG 
ATGGAATCTC TAAGCAAAGT GACAGGAGCA ACGCCTATTG GTTCTCTAAA CCCAATAGTT 
TCTACTACTT ATGGAAGTGT GAAAGATGTG TGCTCTGCAA GATTTGGCTC CAAACATTTT 
TTTCATCTTC TTCCTAATGA GGCCACTGTC TGCACCTTGC TTCTCTGCAG CAGAAATGAC 
ACCGCCTGGG AGGAGCTGAA GCTCACATGT CAAACAGCAA TGCACGTCTT GCAGTTAACA 
ATCAAGGAAC CGTGGGTTTT ATTGGGAGGT GGCTGTACAG AAACACACTT GGCTGCATAT 
GTCAGACACA AGGTTCATCA CGAGGCAGAA GCTATTGTCA GAGATGATGG GTGTACTCAG 
GCAAAGCTGC ATGTTGCTGC TGAAGCATTT TGCAGTGCTC TGGAGTCCGT TGCTGGCTCT 
TTGGAACATG ATGGTGGTGA AATCCTCATT GACACGAAGT ATGGACACCT TTGGTCCTGT 
CAAGCAGATT CTGCCTCTGT TGGTAACTGG TCAGATACGC TGTCACGGTG TGGCTGTGGT 
TTGTACAACA GCCAGGAAGA GCTCAGCTGG TCTGTCTTAA GAAGTACTTA TCATCCTTTT 
GCACCACAAA CCTGCCTTCC ACAGGCAGCT TTGGGCTCAG CCAGTAACCT GACTGTGGAC 
TGCTTCACTG CCAAGCTGAG TGGCTTACAG GTGGCTGTAG AGACAGCCAA TTTGATTTTA 
GATCTTTCAT ATGTCATTGA AGATAAAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238023.1
CDS	778..2490
Translation	MSRLEAKKPSLCKTEPLTSEKVRSTLSVLKGVIASCYGPSGRLKQLHNGLGGCVYTTSQSSALLRNLSVTHPVLKILTSSVQNHVSCFSDCGLFTAILCCNLIENIQRLDLTPATAIKLNKYLLSLCTSYLKSEACSCRIPVDFRSTHTFLSLVHSILTSKPACMLTRKETDHIGALILKAFLLTIPESTEERMVLGKSIIVPLKGQRVTDSTVLPGLLIEASEVQLRRLLPTQKASGLRVALFCTSLSGDFSNAGEGVVVAHYQVSLENAVLEQLLNLGRRLVTDHVDLVLCQKVIHPSLKQFFSERHVMAIDRVGVTLMESLSKVTGATPIGSLNPIVSTTYGSVKDVCSARFGSKHFFHLLPNEATVCTLLLCSRNDTAWEELKLTCQTAMHVLQLTIKEPWVLLGGGCTETHLAAYVRHKVHHEAEAIVRDDGCTQAKLHVAAEAFCSALESVAGSLEHDGGEILIDTKYGHLWSCQADSASVGNWSDTLSRCGCGLYNSQEELSWSVLRSTYHPFAPQTCLPQAALGSASNLTVDCFTAKLSGLQVAVETANLILDLSYVIEDKN

Target ORF information:

RefSeq Version	XM_011239721.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus McKusick-Kaufman syndrome (Mkks), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239721.1

ORF Insert Sequence:

ATGTCTCGGT TAGAAGCTAA GAAGCCATCG TTGTGTAAAA CTGAACCATT GACAAGTGAG 
AAAGTCAGGT CCACACTTTC TGTCTTGAAA GGAGTCATAG CCTCATGCTA TGGCCCTTCA 
GGGAGGCTGA AGCAGCTACA CAATGGATTG GGAGGTTGTG TGTACACAAC CTCACAGTCC 
TCAGCCCTGC TTCGAAACCT TTCAGTCACC CATCCCGTAT TAAAGATCCT AACGTCATCC 
GTGCAGAATC ACGTGTCCTG CTTCAGTGAC TGCGGCTTAT TCACAGCCAT TCTGTGCTGC 
AACTTGATTG AAAATATTCA AAGACTAGAT TTGACACCCG CAACTGCTAT TAAATTAAAT 
AAATACCTCT TGAGTCTCTG TACCAGTTAT CTCAAGTCTG AAGCCTGTAG TTGTCGAATC 
CCAGTTGACT TCAGAAGTAC ACATACCTTC CTCAGCTTGG TACACAGTAT CTTAACAAGC 
AAACCAGCCT GTATGCTCAC CAGAAAGGAA ACAGATCACA TAGGTGCTTT GATTTTGAAA 
GCTTTTTTAC TTACAATTCC AGAAAGTACA GAAGAACGAA TGGTTTTAGG GAAGAGTATA 
ATTGTTCCCT TAAAGGGCCA AAGAGTTACA GATTCTACTG TATTACCTGG ACTACTCATT 
GAAGCATCAG AAGTTCAATT AAGGAGGTTA TTACCCACTC AAAAGGCAAG TGGCCTTAGG 
GTGGCACTCT TCTGTACATC TTTATCTGGA GATTTTTCTA ATGCTGGAGA AGGAGTGGTG 
GTGGCCCATT ACCAAGTGTC CCTTGAGAAT GCAGTTTTAG AGCAGTTGCT TAATCTAGGA 
AGGCGACTAG TCACTGATCA TGTAGACCTT GTCCTGTGCC AAAAAGTAAT CCACCCATCT 
TTGAAACAGT TCTTCAGTGA GCGCCACGTT ATGGCCATCG ACAGAGTTGG GGTGACTCTG 
ATGGAATCTC TAAGCAAAGT GACAGGAGCA ACGCCTATTG GTTCTCTAAA CCCAATAGTT 
TCTACTACTT ATGGAAGTGT GAAAGATGTG TGCTCTGCAA GATTTGGCTC CAAACATTTT 
TTTCATCTTC TTCCTAATGA GGCCACTGTC TGCACCTTGC TTCTCTGCAG CAGAAATGAC 
ACCGCCTGGG AGGAGCTGAA GCTCACATGT CAAACAGCAA TGCACGTCTT GCAGTTAACA 
ATCAAGGAAC CGTGGGTTTT ATTGGGAGGT GGCTGTACAG AAACACACTT GGCTGCATAT 
GTCAGACACA AGGTTCATCA CGAGGCAGAA GCTATTGTCA GAGATGATGG GTGTACTCAG 
GCAAAGCTGC ATGTTGCTGC TGAAGCATTT TGCAGTGCTC TGGAGTCCGT TGCTGGCTCT 
TTGGAACATG ATGGTGGTGA AATCCTCATT GACACGAAGT ATGGACACCT TTGGTCCTGT 
CAAGCAGATT CTGCCTCTGT TGGTAACTGG TCAGATACGC TGTCACGGTG TGGCTGTGGT 
TTGTACAACA GCCAGGAAGA GCTCAGCTGG TCTGTCTTAA GAAGTACTTA TCATCCTTTT 
GCACCACAAA CCTGCCTTCC ACAGGCAGCT TTGGGCTCAG CCAGTAACCT GACTGTGGAC 
TGCTTCACTG CCAAGCTGAG TGGCTTACAG GTGGCTGTAG AGACAGCCAA TTTGATTTTA 
GATCTTTCAT ATGTCATTGA AGATAAAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Human molecular genetics25(14)3011-3028(2016 07)
Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

Regulation of Insulin Receptor Trafficking by Bardet Biedl Syndrome Proteins.
PLoS genetics11(6)e1005311(2015 Jun)
Starks RD,Beyer AM,Guo DF,Boland L,Zhang Q,Sheffield VC,Rahmouni K

Bardet-Biedl syndrome proteins control the cilia length through regulation of actin polymerization.
Human molecular genetics22(19)3858-68(2013 Oct)
Hernandez-Hernandez V,Pravincumar P,Diaz-Font A,May-Simera H,Jenkins D,Knight M,Beales PL

Translational repression of the McKusick-Kaufman syndrome transcript by unique upstream open reading frames encoding mitochondrial proteins with alternative polyadenylation sites.
Biochimica et biophysica acta1830(3)2728-38(2013 Mar)
Akimoto C,Sakashita E,Kasashima K,Kuroiwa K,Tominaga K,Hamamoto T,Endo H

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Mkks cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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