Pias4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pias4
Entrez Gene ID	59004
Full Name	protein inhibitor of activated STAT 4
Synonyms	PIASY,Pias-gamma,Piasg
General protein information	Preferred Names protein inhibitor of activated STAT 4 Names E3 SUMO-protein ligase PIAS4 RING-type E3 ubiquitin transferase PIAS4 protein inhibitor of activated STAT PIASy protein inhibitor of activated STAT protein 4 protein inhibitor of activated STAT protein gamma
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_021501.4	NP_067476.2	E3 SUMO-protein ligase PIAS4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PIAS4	NP_001076951.1
Gallus gallus (chicken)	PIAS4	XP_003642892.2
Homo sapiens (human)	PIAS4	NP_056981.2
Mus musculus (house mouse)	Pias4	NP_067476.2
Rattus norvegicus (Norway rat)	Pias4	NP_001094227.1
Danio rerio (zebrafish)	pias4a	NP_998568.2
Canis lupus familiaris (dog)	PIAS4	XP_542167.4
Xenopus tropicalis (tropical clawed frog)	pias4	NP_001011455.1

Gene Ontology
Bibliography

Related articles in PubMed

Foxp1 regulation of neonatal vocalizations via cortical development.
Usui N, Araujo DJ, Kulkarni A, Co M, Ellegood J, Harper M, Toriumi K, Lerch JP, Konopka G
Genes & development31(20)2039-2055(2017 Oct)

Chromatin remodeling: demethylating H3K4me3 of type I IFNs gene by Rbp2 through interacting with Piasy for transcriptional attenuation.
Yu X, Chen H, Zuo C, Jin X, Yin Y, Wang H, Jin M, Ozato K, Xu S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Sep)

Protein inhibitor of activated STAT 4 (PIAS4) regulates pro-inflammatory transcription in hepatocytes by repressing SIRT1.
Sun L, Fan Z, Chen J, Tian W, Li M, Xu H, Wu X, Fang M, Xia J, Xu Y
Oncotarget7(28)42892-42903(2016 Jul)

Transcriptional repression of SIRT1 by protein inhibitor of activated STAT 4 (PIAS4) in hepatic stellate cells contributes to liver fibrosis.
Sun L, Fan Z, Chen J, Tian W, Li M, Xu H, Wu X, Shao J, Bian Y, Fang M, Xu Y
Scientific reports628432(2016 Jun)

Ubiquitin E3 ligase FIEL1 regulates fibrotic lung injury through SUMO-E3 ligase PIAS4.
Lear T, McKelvey AC, Rajbhandari S, Dunn SR, Coon TA, Connelly W, Zhao JY, Kass DJ, Zhang Y, Liu Y, Chen BB
The Journal of experimental medicine213(6)1029-46(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings indicate that protein inhibitor of activated STAT 4 (PIAS4) mediated SIRT1 repression in response to nutrient surplus contributes to the pathogenesis of Nonalcoholic Steatohepatitis (NASH).
Title: Protein inhibitor of activated STAT 4 (PIAS4) regulates pro-inflammatory transcription in hepatocytes by repressing SIRT1.

new protein isoform encoded by KIAA0317, termed fibrosis-inducing E3 ligase 1 (FIEL1), which potently stimulates the TGFbeta signaling pathway through the site-specific ubiquitination of PIAS4.
Title: Ubiquitin E3 ligase FIEL1 regulates fibrotic lung injury through SUMO-E3 ligase PIAS4.

PKA-induced SREBP1c sumoylation by PIASy.
Title: PIASy-mediated sumoylation of SREBP1c regulates hepatic lipid metabolism upon fasting signaling.

the SUMO E3 ligase PIASy (also known as PIAS4) was induced by hypoxia and prevented Sp1 from binding to the SIRT1 promoter.
Title: PIASy mediates hypoxia-induced SIRT1 transcriptional repression and epithelial-to-mesenchymal transition in ovarian cancer cells.

Piasy represses the synergistic activation of PITX2 with interacting co-factors and Piasy represses Pias1 activation of PITX2 transcriptional activity.
Title: Protein inhibitors of activated STAT (Pias1 and Piasy) differentially regulate pituitary homeobox 2 (PITX2) transcriptional activity.

The following Pias4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pias4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22353	NM_021501.4 Latest version!	Mus musculus protein inhibitor of activated STAT 4 (Pias4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$300.30 $429.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pias4 ( NM_021501.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067476.2 Mus musculus protein inhibitor of activated STAT 4 (Pias4), mRNA.

Price & Availability↑

CloneID	OMu22353
Clone ID Related Accession (Same CDS sequence)	NM_021501.4
Accession Version	NM_021501.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	E3 SUMO-protein ligase PIAS4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC025159.1. On Oct 21, 2007 this sequence version replaced NM_021501.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC025159.1, AF109174.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCAG AGCTGGTGGA GGCCAAAAAC ATGGTGATGA GCTTCCGAGT ATCAGACCTG 
CAGATGCTGC TGGGCTTTGT AGGCCGGAGC AAGAGTGGGC TGAAGCACGA ACTGGTGACC 
AGGGCCTTGC AGCTGGTGCA GTTCGACTGT AGCCCAGAGC TTTTCAAGAA GATCAAAGAG 
CTGTATGAGA CTCGCTATGC CAAGAAGAGT GCAGAGCCCG GCCCACAGGC ACCACGGCCC 
CTGGATCCCC TGGCACTACA CTCCATGCCC AGGACTCCCC TGTCAGGGCC CACCGTGGAC 
TACCCCGTGC TGTACGGGAA GTACTTGAAT GGACTGGGGC GCCTGCCCAC CAAGACCCTT 
AAGCCGGAGG TACGCCTGGT TAAGCTCCCC TTCTTCAACA TGCTGGACGA ATTGCTGAAG 
CCCACTGAAC TGGTCCCACA GAGTGCAGAG AAGCTGCAAG AGAGCCCATG CATCTTCGCG 
CTAACACCTC GGCAGGTGGA GATGATCCGC AACTCCAGGG AGCTGCAGCC TGGAGTGAAA 
GCTGTGCAGG TGGTGCTGAG GATCTGTTAC TCTGATACCA GCTGTCCGCA GGAGGACCAA 
TACCCACCCA ACATCGCTGT GAAGGTCAAC CACAGCTACT GCTCAGTGCC GGGCTACTAT 
CCCTCCAACA AGCCTGGTGT GGAACCTAAG AGGCCCTGCC GCCCCATCAA CCTTACCCAC 
CTCATGTATC TGTCCTCGGC CACCAACCGC ATTACTGTCA CCTGGGGCAA CTATGGCAAG 
AGCTACTCTG TGGCTTTGTA CCTGGTGCGA CAGCTGACCT CCTCAGACCT GCTACAGAGG 
TTGAAGACGA TTGGGGTGAA GCATCCTGAG CTGTGCAAGG CTTTGGTCAA GGAGAAGCTT 
CGCCTAGACC CCGACAGCGA GATCGCCACT ACCGGAGTGC GAGTGTCCCT CATCTGCCCA 
CTGGTGAAGA TGCGCCTCTC GGTGCCCTGC CGTGCAGAGA CCTGCGCACA CCTGCAGTGC 
TTTGATGCTG TGTTCTACCT GCAAATGAAT GAGAAGAAGC CCACCTGGAT GTGCCCTGTG 
TGTGACAAGC CTGCAGCCTA TGACCAGCTC ATCATAGATG GGCTGCTGTC GAAGATCCTG 
AGTGAGTGTG AGGGCGCGGA TGAGATTGAG TTCCTGGCGG AAGGCTCATG GCGTCCAATC 
CGTGCAGAGA AGGAGCCAAG CTGCAGCCCA CAGGGCCCCA TCCTGGTGCT AGGCACCTCG 
GACGCCAATG GGCTCGCACC AGCCTCCAGC ACCCCCGGTA TAGGCAGTGG CCTGAGTGGC 
CCAGGCAGCG CCGGCAGCGG TGCGGGGGCA GCAGGCAGCC TGGAGAATGG CAAGACGGGA 
GCCGACGTGG TGGACCTCAC ACTGGACAGC TCGTCGTCCT CGGAAGATGA AGATGAGGAC 
GAGGACGACG ATGAGGACGA GGACGAGGGC CCCCGGCCGA AGCGCCGCTG CCCGTTCCAG 
AAAGGCCTGG TGCCCGCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067476.2
CDS	10..1533
Translation	MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKSAEPGPQAPRPLDPLALHSMPRTPLSGPTVDYPVLYGKYLNGLGRLPTKTLKPEVRLVKLPFFNMLDELLKPTELVPQSAEKLQESPCIFALTPRQVEMIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSDLLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAAYDQLIIDGLLSKILSECEGADEIEFLAEGSWRPIRAEKEPSCSPQGPILVLGTSDANGLAPASSTPGIGSGLSGPGSAGSGAGAAGSLENGKTGADVVDLTLDSSSSSEDEDEDEDDDEDEDEGPRPKRRCPFQKGLVPAC

Target ORF information:

RefSeq Version	NM_021501.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein inhibitor of activated STAT 4 (Pias4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021501.4

ORF Insert Sequence:

ATGGCGGCAG AGCTGGTGGA GGCCAAAAAC ATGGTGATGA GCTTCCGAGT ATCAGACCTG 
CAGATGCTGC TGGGCTTTGT AGGCCGGAGC AAGAGTGGGC TGAAGCACGA ACTGGTGACC 
AGGGCCTTGC AGCTGGTGCA GTTCGACTGT AGCCCAGAGC TTTTCAAGAA GATCAAAGAG 
CTGTATGAGA CTCGCTATGC CAAGAAGAGT GCAGAGCCCG GCCCACAGGC ACCACGGCCC 
CTGGATCCCC TGGCACTACA CTCCATGCCC AGGACTCCCC TGTCAGGGCC CACCGTGGAC 
TACCCCGTGC TGTACGGGAA GTACTTGAAT GGACTGGGGC GCCTGCCCAC CAAGACCCTT 
AAGCCGGAGG TACGCCTGGT TAAGCTCCCC TTCTTCAACA TGCTGGACGA ATTGCTGAAG 
CCCACTGAAC TGGTCCCACA GAGTGCAGAG AAGCTGCAAG AGAGCCCATG CATCTTCGCG 
CTAACACCTC GGCAGGTGGA GATGATCCGC AACTCCAGGG AGCTGCAGCC TGGAGTGAAA 
GCTGTGCAGG TGGTGCTGAG GATCTGTTAC TCTGATACCA GCTGTCCGCA GGAGGACCAA 
TACCCACCCA ACATCGCTGT GAAGGTCAAC CACAGCTACT GCTCAGTGCC GGGCTACTAT 
CCCTCCAACA AGCCTGGTGT GGAACCTAAG AGGCCCTGCC GCCCCATCAA CCTTACCCAC 
CTCATGTATC TGTCCTCGGC CACCAACCGC ATTACTGTCA CCTGGGGCAA CTATGGCAAG 
AGCTACTCTG TGGCTTTGTA CCTGGTGCGA CAGCTGACCT CCTCAGACCT GCTACAGAGG 
TTGAAGACGA TTGGGGTGAA GCATCCTGAG CTGTGCAAGG CTTTGGTCAA GGAGAAGCTT 
CGCCTAGACC CCGACAGCGA GATCGCCACT ACCGGAGTGC GAGTGTCCCT CATCTGCCCA 
CTGGTGAAGA TGCGCCTCTC GGTGCCCTGC CGTGCAGAGA CCTGCGCACA CCTGCAGTGC 
TTTGATGCTG TGTTCTACCT GCAAATGAAT GAGAAGAAGC CCACCTGGAT GTGCCCTGTG 
TGTGACAAGC CTGCAGCCTA TGACCAGCTC ATCATAGATG GGCTGCTGTC GAAGATCCTG 
AGTGAGTGTG AGGGCGCGGA TGAGATTGAG TTCCTGGCGG AAGGCTCATG GCGTCCAATC 
CGTGCAGAGA AGGAGCCAAG CTGCAGCCCA CAGGGCCCCA TCCTGGTGCT AGGCACCTCG 
GACGCCAATG GGCTCGCACC AGCCTCCAGC ACCCCCGGTA TAGGCAGTGG CCTGAGTGGC 
CCAGGCAGCG CCGGCAGCGG TGCGGGGGCA GCAGGCAGCC TGGAGAATGG CAAGACGGGA 
GCCGACGTGG TGGACCTCAC ACTGGACAGC TCGTCGTCCT CGGAAGATGA AGATGAGGAC 
GAGGACGACG ATGAGGACGA GGACGAGGGC CCCCGGCCGA AGCGCCGCTG CCCGTTCCAG 
AAAGGCCTGG TGCCCGCGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Foxp1 regulation of neonatal vocalizations via cortical development.
Genes & development31(20)2039-2055(2017 Oct)
Usui N,Araujo DJ,Kulkarni A,Co M,Ellegood J,Harper M,Toriumi K,Lerch JP,Konopka G

Chromatin remodeling: demethylating H3K4me3 of type I IFNs gene by Rbp2 through interacting with Piasy for transcriptional attenuation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Sep)
Yu X,Chen H,Zuo C,Jin X,Yin Y,Wang H,Jin M,Ozato K,Xu S

Protein inhibitor of activated STAT 4 (PIAS4) regulates pro-inflammatory transcription in hepatocytes by repressing SIRT1.
Oncotarget7(28)42892-42903(2016 Jul)
Sun L,Fan Z,Chen J,Tian W,Li M,Xu H,Wu X,Fang M,Xia J,Xu Y

Transcriptional repression of SIRT1 by protein inhibitor of activated STAT 4 (PIAS4) in hepatic stellate cells contributes to liver fibrosis.
Scientific reports628432(2016 Jun)
Sun L,Fan Z,Chen J,Tian W,Li M,Xu H,Wu X,Shao J,Bian Y,Fang M,Xu Y

Ubiquitin E3 ligase FIEL1 regulates fibrotic lung injury through SUMO-E3 ligase PIAS4.
The Journal of experimental medicine213(6)1029-46(2016 May)
Lear T,McKelvey AC,Rajbhandari S,Dunn SR,Coon TA,Connelly W,Zhao JY,Kass DJ,Zhang Y,Liu Y,Chen BB

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Pias4 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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