Myot cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myot
Entrez Gene ID	58916
Full Name	myotilin
Synonyms	5530402I04Rik,Ttid
General protein information	Preferred Names myotilin Names myotilin myofibrillar titin-like Ig domains protein titan-like protein titin immunoglobulin domain protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526133.3	XP_006526196.1	myotilin isoform X2
NM_001033621.4	NP_001028793.1	myotilin
XM_006526133.4	XP_006526196.1	myotilin isoform X2
NM_001033621.3	NP_001028793.1	myotilin
XM_006526131.3	XP_006526194.1	myotilin isoform X1
XM_006526132.3	XP_006526195.1	myotilin isoform X1
XM_006526132.4	XP_006526195.1	myotilin isoform X1
XM_006526131.4	XP_006526194.1	myotilin isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MYOT	NP_001068803.1
Rattus norvegicus (Norway rat)	Myot	NP_001099618.1
Rattus norvegicus (Norway rat)	LOC100909761	XP_006255053.1
Pan troglodytes (chimpanzee)	MYOT	XP_517948.2
Xenopus tropicalis (tropical clawed frog)	myot	NP_001017159.2
Canis lupus familiaris (dog)	MYOT	XP_003639423.1
Rattus norvegicus (Norway rat)	LOC100912306	XP_006244741.1
Homo sapiens (human)	MYOT	NP_006781.1
Mus musculus (house mouse)	Myot	NP_001028793.1
	MYOT	XP_001106921.2
Gallus gallus (chicken)	MYOT	XP_414618.4
Danio rerio (zebrafish)	LOC100329862	XP_002664374.3

Gene Ontology
Bibliography

Related articles in PubMed

Abnormal Mammary Development in 129:STAT1-Null Mice is Stroma-Dependent.
Chen JQ, Mori H, Cardiff RD, Trott JF, Hovey RC, Hubbard NE, Engelberg JA, Tepper CG, Willis BJ, Khan IH, Ravindran RK, Chan SR, Schreiber RD, Borowsky AD
PloS one10(6)e0129895(2015)

TAF7L modulates brown adipose tissue formation.
Zhou H, Wan B, Grubisic I, Kaplan T, Tjian R
eLife3(2014 May)

A class III PDZ binding motif in the myotilin and FATZ families binds enigma family proteins: a common link for Z-disc myopathies.
von Nandelstadh P, Ismail M, Gardin C, Suila H, Zara I, Belgrano A, Valle G, Carpen O, Faulkner G
Molecular and cellular biology29(3)822-34(2009 Feb)

Maintenance of muscle mass, fiber size, and contractile function in mice lacking the Z-disc protein myotilin.
Ochala J, Carp?n O, Larsson L
Upsala journal of medical sciences114(4)235-41(2009)

Myotilin overexpression enhances myopathology in the LGMD1A mouse model.
Garvey SM, Liu Y, Miller SE, Hauser MA
Muscle & nerve37(5)663-7(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Myotilin does not have a significant influence on muscle mass, muscle fiber size, or regulation of muscle contraction. Alternatively, compensatory over-expressions of other elements may compensate for the lack of myotilin.
Title: Maintenance of muscle mass, fiber size, and contractile function in mice lacking the Z-disc protein myotilin.

In single-transgenic mutant mice, double-transgenic mice overexpressing myotilin showed more severe muscle degeneration, enhanced myofibrillar aggregation, and earlier onset of aggregation.
Title: Myotilin overexpression enhances myopathology in the LGMD1A mouse model.

either myotilin does not participate in muscle development and basal function maintenance or other proteins serve as structural and functional compensatory molecules when myotilin is absent
Title: Targeted deletion of the muscular dystrophy gene myotilin does not perturb muscle structure or function in mice.

features suggest an important role for myotilin in sarcomere organization.
Title: Characterization of mouse myotilin and its promoter.

The following Myot gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myot cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12726	NM_001033621.4 Latest version!	Mus musculus myotilin (Myot), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu12726	XM_006526133.4 Latest version!	Mus musculus myotilin (Myot), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu54720	XM_006526131.4 Latest version!	Mus musculus myotilin (Myot), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu54720	XM_006526132.4 Latest version!	Mus musculus myotilin (Myot), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu12726	XM_006526133.3	Mus musculus myotilin (Myot), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu12726	NM_001033621.3	Mus musculus myotilin (Myot), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu54720	XM_006526132.3	Mus musculus myotilin (Myot), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu54720	XM_006526131.3	Mus musculus myotilin (Myot), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myot ( NM_001033621.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028793.1 Mus musculus myotilin (Myot), mRNA.

Price & Availability↑

CloneID	OMu12726
Clone ID Related Accession (Same CDS sequence)	XM_006526133.3 , NM_001033621.4 , XM_006526133.4 , NM_001033621.3
Accession Version	NM_001033621.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	myotilin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132130.3. On Jul 18, 2018 this sequence version replaced NM_001033621.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF230979.2, AK017447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CATAACCCAG AGCAAGCACG GCTGCACGTT 
CCCACATCAC AAGTAAGAAG TAGATCGTCC TCCAGAGCAG AGGCCAATGA TCAGGATGCC 
ATCCAGGAGA AGTTTTACCC ACCACGCTTC ATTCAAGTGC CAGAAAACAT GTCGATCGAA 
GAGGGGAGGT TCTGCCGAAT GGACTTTAAA GTAAGTGGAC TGCCAGCCCC TGATGTGTCA 
TGGTATCTAA ACGGGAGACC AGTGCAATCA GATGAGTTGC ACAAAATGAT AGTGTCTGAG 
AAGGGTTTCC ACTCCCTCAT CTTCGAAGTG GTCAGAGCAT CAGATGCAGG GCCATATGCA 
TGCGTGGCCA GGAACAGAGC TGGAGAAGCC ACCTTCACAG TGCAGCTGGA TGTTCTGGCA 
AAAGAGCATA AAAGAGCACC GATGTTTATC TTCAAACCAC AGAGTAAGAA AGTTTTTGAG 
GGAGAGACCG TGAAGCTGGA GTGCCAGATC TCAGCTATCC CTCCACCAAA GCTGTTCTGG 
AAAAGAAACA ATGAAATGGT CCAGTTCAAC ACTGATCGGA TAAGCTTATA TCATGACAAC 
GCCGGAAGAG TAACTTTACT GATAAAAGAT GTAAACAAGA AGGACGCTGG GTGGTATACA 
GTGTCAGCAG TCAATGAAGC TGGTGTGACC ACGTGTAACA CACGATTAGA TGTCACAGCC 
CGTCCAATCC AAACTCTCCC AGCTCCTAAG CAGCTGCGTG TTCGACCAAC ATTCAGCAAA 
TATTTAGCGC TCAATGGGAG AGGTTTGGAT GTGAAACAAG CTTTTAACCC TGAAGGAGAA 
TTTCAGCGCC TGGCAGCTCA ATCCGGACTC TACGAAAGTG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028793.1
CDS	275..1765
Translation	MFNYERPKHFIQPQNPCGSRLQPPGPEVSGFPSQTKQSSIVIQPRQCTEQRFSASSTVSSHITVSSSAYPAPQQLAGPNPGQKVTATYNQSPASFLSSILPSQPDYCNSKIPSTVDSNYQQSSVNQPVNAMSSQAANARPTPKTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKMARRLLGPQNAAAVFQAQNSDVQDSPQHNPEQARLHVPTSQVRSRSSSRAEANDQDAIQEKFYPPRFIQVPENMSIEEGRFCRMDFKVSGLPAPDVSWYLNGRPVQSDELHKMIVSEKGFHSLIFEVVRASDAGPYACVARNRAGEATFTVQLDVLAKEHKRAPMFIFKPQSKKVFEGETVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYHDNAGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPIQTLPAPKQLRVRPTFSKYLALNGRGLDVKQAFNPEGEFQRLAAQSGLYESEEL

Target ORF information:

RefSeq Version	NM_001033621.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myotilin (Myot), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033621.4

ORF Insert Sequence:

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CATAACCCAG AGCAAGCACG GCTGCACGTT 
CCCACATCAC AAGTAAGAAG TAGATCGTCC TCCAGAGCAG AGGCCAATGA TCAGGATGCC 
ATCCAGGAGA AGTTTTACCC ACCACGCTTC ATTCAAGTGC CAGAAAACAT GTCGATCGAA 
GAGGGGAGGT TCTGCCGAAT GGACTTTAAA GTAAGTGGAC TGCCAGCCCC TGATGTGTCA 
TGGTATCTAA ACGGGAGACC AGTGCAATCA GATGAGTTGC ACAAAATGAT AGTGTCTGAG 
AAGGGTTTCC ACTCCCTCAT CTTCGAAGTG GTCAGAGCAT CAGATGCAGG GCCATATGCA 
TGCGTGGCCA GGAACAGAGC TGGAGAAGCC ACCTTCACAG TGCAGCTGGA TGTTCTGGCA 
AAAGAGCATA AAAGAGCACC GATGTTTATC TTCAAACCAC AGAGTAAGAA AGTTTTTGAG 
GGAGAGACCG TGAAGCTGGA GTGCCAGATC TCAGCTATCC CTCCACCAAA GCTGTTCTGG 
AAAAGAAACA ATGAAATGGT CCAGTTCAAC ACTGATCGGA TAAGCTTATA TCATGACAAC 
GCCGGAAGAG TAACTTTACT GATAAAAGAT GTAAACAAGA AGGACGCTGG GTGGTATACA 
GTGTCAGCAG TCAATGAAGC TGGTGTGACC ACGTGTAACA CACGATTAGA TGTCACAGCC 
CGTCCAATCC AAACTCTCCC AGCTCCTAAG CAGCTGCGTG TTCGACCAAC ATTCAGCAAA 
TATTTAGCGC TCAATGGGAG AGGTTTGGAT GTGAAACAAG CTTTTAACCC TGAAGGAGAA 
TTTCAGCGCC TGGCAGCTCA ATCCGGACTC TACGAAAGTG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myot ( NM_001033621.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028793.1 Mus musculus myotilin (Myot), mRNA.

Price & Availability↑

CloneID	OMu12726
Clone ID Related Accession (Same CDS sequence)	XM_006526133.3 , NM_001033621.4 , XM_006526133.4 , NM_001033621.3
Accession Version	NM_001033621.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Mus musculus(house mouse)
Product	myotilin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC132130.3. On May 2, 2007 this sequence version replaced NM_001033621.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK017447.1, BC016214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CATAACCCAG AGCAAGCACG GCTGCACGTT 
CCCACATCAC AAGTAAGAAG TAGATCGTCC TCCAGAGCAG AGGCCAATGA TCAGGATGCC 
ATCCAGGAGA AGTTTTACCC ACCACGCTTC ATTCAAGTGC CAGAAAACAT GTCGATCGAA 
GAGGGGAGGT TCTGCCGAAT GGACTTTAAA GTAAGTGGAC TGCCAGCCCC TGATGTGTCA 
TGGTATCTAA ACGGGAGACC AGTGCAATCA GATGAGTTGC ACAAAATGAT AGTGTCTGAG 
AAGGGTTTCC ACTCCCTCAT CTTCGAAGTG GTCAGAGCAT CAGATGCAGG GCCATATGCA 
TGCGTGGCCA GGAACAGAGC TGGAGAAGCC ACCTTCACAG TGCAGCTGGA TGTTCTGGCA 
AAAGAGCATA AAAGAGCACC GATGTTTATC TTCAAACCAC AGAGTAAGAA AGTTTTTGAG 
GGAGAGACCG TGAAGCTGGA GTGCCAGATC TCAGCTATCC CTCCACCAAA GCTGTTCTGG 
AAAAGAAACA ATGAAATGGT CCAGTTCAAC ACTGATCGGA TAAGCTTATA TCATGACAAC 
GCCGGAAGAG TAACTTTACT GATAAAAGAT GTAAACAAGA AGGACGCTGG GTGGTATACA 
GTGTCAGCAG TCAATGAAGC TGGTGTGACC ACGTGTAACA CACGATTAGA TGTCACAGCC 
CGTCCAATCC AAACTCTCCC AGCTCCTAAG CAGCTGCGTG TTCGACCAAC ATTCAGCAAA 
TATTTAGCGC TCAATGGGAG AGGTTTGGAT GTGAAACAAG CTTTTAACCC TGAAGGAGAA 
TTTCAGCGCC TGGCAGCTCA ATCCGGACTC TACGAAAGTG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028793.1
CDS	275..1765
Translation	MFNYERPKHFIQPQNPCGSRLQPPGPEVSGFPSQTKQSSIVIQPRQCTEQRFSASSTVSSHITVSSSAYPAPQQLAGPNPGQKVTATYNQSPASFLSSILPSQPDYCNSKIPSTVDSNYQQSSVNQPVNAMSSQAANARPTPKTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKMARRLLGPQNAAAVFQAQNSDVQDSPQHNPEQARLHVPTSQVRSRSSSRAEANDQDAIQEKFYPPRFIQVPENMSIEEGRFCRMDFKVSGLPAPDVSWYLNGRPVQSDELHKMIVSEKGFHSLIFEVVRASDAGPYACVARNRAGEATFTVQLDVLAKEHKRAPMFIFKPQSKKVFEGETVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYHDNAGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPIQTLPAPKQLRVRPTFSKYLALNGRGLDVKQAFNPEGEFQRLAAQSGLYESEEL

Target ORF information:

RefSeq Version	NM_001033621.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myotilin (Myot), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033621.3

ORF Insert Sequence:

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CATAACCCAG AGCAAGCACG GCTGCACGTT 
CCCACATCAC AAGTAAGAAG TAGATCGTCC TCCAGAGCAG AGGCCAATGA TCAGGATGCC 
ATCCAGGAGA AGTTTTACCC ACCACGCTTC ATTCAAGTGC CAGAAAACAT GTCGATCGAA 
GAGGGGAGGT TCTGCCGAAT GGACTTTAAA GTAAGTGGAC TGCCAGCCCC TGATGTGTCA 
TGGTATCTAA ACGGGAGACC AGTGCAATCA GATGAGTTGC ACAAAATGAT AGTGTCTGAG 
AAGGGTTTCC ACTCCCTCAT CTTCGAAGTG GTCAGAGCAT CAGATGCAGG GCCATATGCA 
TGCGTGGCCA GGAACAGAGC TGGAGAAGCC ACCTTCACAG TGCAGCTGGA TGTTCTGGCA 
AAAGAGCATA AAAGAGCACC GATGTTTATC TTCAAACCAC AGAGTAAGAA AGTTTTTGAG 
GGAGAGACCG TGAAGCTGGA GTGCCAGATC TCAGCTATCC CTCCACCAAA GCTGTTCTGG 
AAAAGAAACA ATGAAATGGT CCAGTTCAAC ACTGATCGGA TAAGCTTATA TCATGACAAC 
GCCGGAAGAG TAACTTTACT GATAAAAGAT GTAAACAAGA AGGACGCTGG GTGGTATACA 
GTGTCAGCAG TCAATGAAGC TGGTGTGACC ACGTGTAACA CACGATTAGA TGTCACAGCC 
CGTCCAATCC AAACTCTCCC AGCTCCTAAG CAGCTGCGTG TTCGACCAAC ATTCAGCAAA 
TATTTAGCGC TCAATGGGAG AGGTTTGGAT GTGAAACAAG CTTTTAACCC TGAAGGAGAA 
TTTCAGCGCC TGGCAGCTCA ATCCGGACTC TACGAAAGTG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myot ( XM_006526133.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526196.1 Mus musculus myotilin (Myot), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu12726
Clone ID Related Accession (Same CDS sequence)	XM_006526133.3 , NM_001033621.4 , XM_006526133.4 , NM_001033621.3
Accession Version	XM_006526133.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myotilin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526133.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CATAACCCAG AGCAAGCACG GCTGCACGTT 
CCCACATCAC AAGTAAGAAG TAGATCGTCC TCCAGAGCAG AGGCCAATGA TCAGGATGCC 
ATCCAGGAGA AGTTTTACCC ACCACGCTTC ATTCAAGTGC CAGAAAACAT GTCGATCGAA 
GAGGGGAGGT TCTGCCGAAT GGACTTTAAA GTAAGTGGAC TGCCAGCCCC TGATGTGTCA 
TGGTATCTAA ACGGGAGACC AGTGCAATCA GATGAGTTGC ACAAAATGAT AGTGTCTGAG 
AAGGGTTTCC ACTCCCTCAT CTTCGAAGTG GTCAGAGCAT CAGATGCAGG GCCATATGCA 
TGCGTGGCCA GGAACAGAGC TGGAGAAGCC ACCTTCACAG TGCAGCTGGA TGTTCTGGCA 
AAAGAGCATA AAAGAGCACC GATGTTTATC TTCAAACCAC AGAGTAAGAA AGTTTTTGAG 
GGAGAGACCG TGAAGCTGGA GTGCCAGATC TCAGCTATCC CTCCACCAAA GCTGTTCTGG 
AAAAGAAACA ATGAAATGGT CCAGTTCAAC ACTGATCGGA TAAGCTTATA TCATGACAAC 
GCCGGAAGAG TAACTTTACT GATAAAAGAT GTAAACAAGA AGGACGCTGG GTGGTATACA 
GTGTCAGCAG TCAATGAAGC TGGTGTGACC ACGTGTAACA CACGATTAGA TGTCACAGCC 
CGTCCAATCC AAACTCTCCC AGCTCCTAAG CAGCTGCGTG TTCGACCAAC ATTCAGCAAA 
TATTTAGCGC TCAATGGGAG AGGTTTGGAT GTGAAACAAG CTTTTAACCC TGAAGGAGAA 
TTTCAGCGCC TGGCAGCTCA ATCCGGACTC TACGAAAGTG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526196.1
CDS	169..1659
Translation	MFNYERPKHFIQPQNPCGSRLQPPGPEVSGFPSQTKQSSIVIQPRQCTEQRFSASSTVSSHITVSSSAYPAPQQLAGPNPGQKVTATYNQSPASFLSSILPSQPDYCNSKIPSTVDSNYQQSSVNQPVNAMSSQAANARPTPKTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKMARRLLGPQNAAAVFQAQNSDVQDSPQHNPEQARLHVPTSQVRSRSSSRAEANDQDAIQEKFYPPRFIQVPENMSIEEGRFCRMDFKVSGLPAPDVSWYLNGRPVQSDELHKMIVSEKGFHSLIFEVVRASDAGPYACVARNRAGEATFTVQLDVLAKEHKRAPMFIFKPQSKKVFEGETVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYHDNAGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPIQTLPAPKQLRVRPTFSKYLALNGRGLDVKQAFNPEGEFQRLAAQSGLYESEEL

Target ORF information:

RefSeq Version	XM_006526133.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myotilin (Myot), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526133.3

ORF Insert Sequence:

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CATAACCCAG AGCAAGCACG GCTGCACGTT 
CCCACATCAC AAGTAAGAAG TAGATCGTCC TCCAGAGCAG AGGCCAATGA TCAGGATGCC 
ATCCAGGAGA AGTTTTACCC ACCACGCTTC ATTCAAGTGC CAGAAAACAT GTCGATCGAA 
GAGGGGAGGT TCTGCCGAAT GGACTTTAAA GTAAGTGGAC TGCCAGCCCC TGATGTGTCA 
TGGTATCTAA ACGGGAGACC AGTGCAATCA GATGAGTTGC ACAAAATGAT AGTGTCTGAG 
AAGGGTTTCC ACTCCCTCAT CTTCGAAGTG GTCAGAGCAT CAGATGCAGG GCCATATGCA 
TGCGTGGCCA GGAACAGAGC TGGAGAAGCC ACCTTCACAG TGCAGCTGGA TGTTCTGGCA 
AAAGAGCATA AAAGAGCACC GATGTTTATC TTCAAACCAC AGAGTAAGAA AGTTTTTGAG 
GGAGAGACCG TGAAGCTGGA GTGCCAGATC TCAGCTATCC CTCCACCAAA GCTGTTCTGG 
AAAAGAAACA ATGAAATGGT CCAGTTCAAC ACTGATCGGA TAAGCTTATA TCATGACAAC 
GCCGGAAGAG TAACTTTACT GATAAAAGAT GTAAACAAGA AGGACGCTGG GTGGTATACA 
GTGTCAGCAG TCAATGAAGC TGGTGTGACC ACGTGTAACA CACGATTAGA TGTCACAGCC 
CGTCCAATCC AAACTCTCCC AGCTCCTAAG CAGCTGCGTG TTCGACCAAC ATTCAGCAAA 
TATTTAGCGC TCAATGGGAG AGGTTTGGAT GTGAAACAAG CTTTTAACCC TGAAGGAGAA 
TTTCAGCGCC TGGCAGCTCA ATCCGGACTC TACGAAAGTG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myot ( XM_006526131.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526194.1 Mus musculus myotilin (Myot), transcript variant X1, mRNA.

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CloneID	OMu54720
Clone ID Related Accession (Same CDS sequence)	XM_006526131.3 , XM_006526132.3 , XM_006526132.4 , XM_006526131.4
Accession Version	XM_006526131.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myotilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CAGCATAACC CAGAGCAAGC ACGGCTGCAC 
GTTCCCACAT CACAAGTAAG AAGTAGATCG TCCTCCAGAG CAGAGGCCAA TGATCAGGAT 
GCCATCCAGG AGAAGTTTTA CCCACCACGC TTCATTCAAG TGCCAGAAAA CATGTCGATC 
GAAGAGGGGA GGTTCTGCCG AATGGACTTT AAAGTAAGTG GACTGCCAGC CCCTGATGTG 
TCATGGTATC TAAACGGGAG ACCAGTGCAA TCAGATGAGT TGCACAAAAT GATAGTGTCT 
GAGAAGGGTT TCCACTCCCT CATCTTCGAA GTGGTCAGAG CATCAGATGC AGGGCCATAT 
GCATGCGTGG CCAGGAACAG AGCTGGAGAA GCCACCTTCA CAGTGCAGCT GGATGTTCTG 
GCAAAAGAGC ATAAAAGAGC ACCGATGTTT ATCTTCAAAC CACAGAGTAA GAAAGTTTTT 
GAGGGAGAGA CCGTGAAGCT GGAGTGCCAG ATCTCAGCTA TCCCTCCACC AAAGCTGTTC 
TGGAAAAGAA ACAATGAAAT GGTCCAGTTC AACACTGATC GGATAAGCTT ATATCATGAC 
AACGCCGGAA GAGTAACTTT ACTGATAAAA GATGTAAACA AGAAGGACGC TGGGTGGTAT 
ACAGTGTCAG CAGTCAATGA AGCTGGTGTG ACCACGTGTA ACACACGATT AGATGTCACA 
GCCCGTCCAA TCCAAACTCT CCCAGCTCCT AAGCAGCTGC GTGTTCGACC AACATTCAGC 
AAATATTTAG CGCTCAATGG GAGAGGTTTG GATGTGAAAC AAGCTTTTAA CCCTGAAGGA 
GAATTTCAGC GCCTGGCAGC TCAATCCGGA CTCTACGAAA GTGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526194.1
CDS	292..1785
Translation	MFNYERPKHFIQPQNPCGSRLQPPGPEVSGFPSQTKQSSIVIQPRQCTEQRFSASSTVSSHITVSSSAYPAPQQLAGPNPGQKVTATYNQSPASFLSSILPSQPDYCNSKIPSTVDSNYQQSSVNQPVNAMSSQAANARPTPKTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKMARRLLGPQNAAAVFQAQNSDVQDSPQQHNPEQARLHVPTSQVRSRSSSRAEANDQDAIQEKFYPPRFIQVPENMSIEEGRFCRMDFKVSGLPAPDVSWYLNGRPVQSDELHKMIVSEKGFHSLIFEVVRASDAGPYACVARNRAGEATFTVQLDVLAKEHKRAPMFIFKPQSKKVFEGETVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYHDNAGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPIQTLPAPKQLRVRPTFSKYLALNGRGLDVKQAFNPEGEFQRLAAQSGLYESEEL

Target ORF information:

RefSeq Version	XM_006526131.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myotilin (Myot), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526131.3

ORF Insert Sequence:

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CAGCATAACC CAGAGCAAGC ACGGCTGCAC 
GTTCCCACAT CACAAGTAAG AAGTAGATCG TCCTCCAGAG CAGAGGCCAA TGATCAGGAT 
GCCATCCAGG AGAAGTTTTA CCCACCACGC TTCATTCAAG TGCCAGAAAA CATGTCGATC 
GAAGAGGGGA GGTTCTGCCG AATGGACTTT AAAGTAAGTG GACTGCCAGC CCCTGATGTG 
TCATGGTATC TAAACGGGAG ACCAGTGCAA TCAGATGAGT TGCACAAAAT GATAGTGTCT 
GAGAAGGGTT TCCACTCCCT CATCTTCGAA GTGGTCAGAG CATCAGATGC AGGGCCATAT 
GCATGCGTGG CCAGGAACAG AGCTGGAGAA GCCACCTTCA CAGTGCAGCT GGATGTTCTG 
GCAAAAGAGC ATAAAAGAGC ACCGATGTTT ATCTTCAAAC CACAGAGTAA GAAAGTTTTT 
GAGGGAGAGA CCGTGAAGCT GGAGTGCCAG ATCTCAGCTA TCCCTCCACC AAAGCTGTTC 
TGGAAAAGAA ACAATGAAAT GGTCCAGTTC AACACTGATC GGATAAGCTT ATATCATGAC 
AACGCCGGAA GAGTAACTTT ACTGATAAAA GATGTAAACA AGAAGGACGC TGGGTGGTAT 
ACAGTGTCAG CAGTCAATGA AGCTGGTGTG ACCACGTGTA ACACACGATT AGATGTCACA 
GCCCGTCCAA TCCAAACTCT CCCAGCTCCT AAGCAGCTGC GTGTTCGACC AACATTCAGC 
AAATATTTAG CGCTCAATGG GAGAGGTTTG GATGTGAAAC AAGCTTTTAA CCCTGAAGGA 
GAATTTCAGC GCCTGGCAGC TCAATCCGGA CTCTACGAAA GTGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myot ( XM_006526132.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526195.1 Mus musculus myotilin (Myot), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54720
Clone ID Related Accession (Same CDS sequence)	XM_006526131.3 , XM_006526132.3 , XM_006526132.4 , XM_006526131.4
Accession Version	XM_006526132.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myotilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CAGCATAACC CAGAGCAAGC ACGGCTGCAC 
GTTCCCACAT CACAAGTAAG AAGTAGATCG TCCTCCAGAG CAGAGGCCAA TGATCAGGAT 
GCCATCCAGG AGAAGTTTTA CCCACCACGC TTCATTCAAG TGCCAGAAAA CATGTCGATC 
GAAGAGGGGA GGTTCTGCCG AATGGACTTT AAAGTAAGTG GACTGCCAGC CCCTGATGTG 
TCATGGTATC TAAACGGGAG ACCAGTGCAA TCAGATGAGT TGCACAAAAT GATAGTGTCT 
GAGAAGGGTT TCCACTCCCT CATCTTCGAA GTGGTCAGAG CATCAGATGC AGGGCCATAT 
GCATGCGTGG CCAGGAACAG AGCTGGAGAA GCCACCTTCA CAGTGCAGCT GGATGTTCTG 
GCAAAAGAGC ATAAAAGAGC ACCGATGTTT ATCTTCAAAC CACAGAGTAA GAAAGTTTTT 
GAGGGAGAGA CCGTGAAGCT GGAGTGCCAG ATCTCAGCTA TCCCTCCACC AAAGCTGTTC 
TGGAAAAGAA ACAATGAAAT GGTCCAGTTC AACACTGATC GGATAAGCTT ATATCATGAC 
AACGCCGGAA GAGTAACTTT ACTGATAAAA GATGTAAACA AGAAGGACGC TGGGTGGTAT 
ACAGTGTCAG CAGTCAATGA AGCTGGTGTG ACCACGTGTA ACACACGATT AGATGTCACA 
GCCCGTCCAA TCCAAACTCT CCCAGCTCCT AAGCAGCTGC GTGTTCGACC AACATTCAGC 
AAATATTTAG CGCTCAATGG GAGAGGTTTG GATGTGAAAC AAGCTTTTAA CCCTGAAGGA 
GAATTTCAGC GCCTGGCAGC TCAATCCGGA CTCTACGAAA GTGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526195.1
CDS	170..1663
Translation	MFNYERPKHFIQPQNPCGSRLQPPGPEVSGFPSQTKQSSIVIQPRQCTEQRFSASSTVSSHITVSSSAYPAPQQLAGPNPGQKVTATYNQSPASFLSSILPSQPDYCNSKIPSTVDSNYQQSSVNQPVNAMSSQAANARPTPKTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKMARRLLGPQNAAAVFQAQNSDVQDSPQQHNPEQARLHVPTSQVRSRSSSRAEANDQDAIQEKFYPPRFIQVPENMSIEEGRFCRMDFKVSGLPAPDVSWYLNGRPVQSDELHKMIVSEKGFHSLIFEVVRASDAGPYACVARNRAGEATFTVQLDVLAKEHKRAPMFIFKPQSKKVFEGETVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYHDNAGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPIQTLPAPKQLRVRPTFSKYLALNGRGLDVKQAFNPEGEFQRLAAQSGLYESEEL

Target ORF information:

RefSeq Version	XM_006526132.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myotilin (Myot), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526132.3

ORF Insert Sequence:

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CAGCATAACC CAGAGCAAGC ACGGCTGCAC 
GTTCCCACAT CACAAGTAAG AAGTAGATCG TCCTCCAGAG CAGAGGCCAA TGATCAGGAT 
GCCATCCAGG AGAAGTTTTA CCCACCACGC TTCATTCAAG TGCCAGAAAA CATGTCGATC 
GAAGAGGGGA GGTTCTGCCG AATGGACTTT AAAGTAAGTG GACTGCCAGC CCCTGATGTG 
TCATGGTATC TAAACGGGAG ACCAGTGCAA TCAGATGAGT TGCACAAAAT GATAGTGTCT 
GAGAAGGGTT TCCACTCCCT CATCTTCGAA GTGGTCAGAG CATCAGATGC AGGGCCATAT 
GCATGCGTGG CCAGGAACAG AGCTGGAGAA GCCACCTTCA CAGTGCAGCT GGATGTTCTG 
GCAAAAGAGC ATAAAAGAGC ACCGATGTTT ATCTTCAAAC CACAGAGTAA GAAAGTTTTT 
GAGGGAGAGA CCGTGAAGCT GGAGTGCCAG ATCTCAGCTA TCCCTCCACC AAAGCTGTTC 
TGGAAAAGAA ACAATGAAAT GGTCCAGTTC AACACTGATC GGATAAGCTT ATATCATGAC 
AACGCCGGAA GAGTAACTTT ACTGATAAAA GATGTAAACA AGAAGGACGC TGGGTGGTAT 
ACAGTGTCAG CAGTCAATGA AGCTGGTGTG ACCACGTGTA ACACACGATT AGATGTCACA 
GCCCGTCCAA TCCAAACTCT CCCAGCTCCT AAGCAGCTGC GTGTTCGACC AACATTCAGC 
AAATATTTAG CGCTCAATGG GAGAGGTTTG GATGTGAAAC AAGCTTTTAA CCCTGAAGGA 
GAATTTCAGC GCCTGGCAGC TCAATCCGGA CTCTACGAAA GTGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myot ( XM_006526133.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526196.1 Mus musculus myotilin (Myot), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu12726
Clone ID Related Accession (Same CDS sequence)	XM_006526133.3 , NM_001033621.4 , XM_006526133.4 , NM_001033621.3
Accession Version	XM_006526133.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myotilin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526133.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CATAACCCAG AGCAAGCACG GCTGCACGTT 
CCCACATCAC AAGTAAGAAG TAGATCGTCC TCCAGAGCAG AGGCCAATGA TCAGGATGCC 
ATCCAGGAGA AGTTTTACCC ACCACGCTTC ATTCAAGTGC CAGAAAACAT GTCGATCGAA 
GAGGGGAGGT TCTGCCGAAT GGACTTTAAA GTAAGTGGAC TGCCAGCCCC TGATGTGTCA 
TGGTATCTAA ACGGGAGACC AGTGCAATCA GATGAGTTGC ACAAAATGAT AGTGTCTGAG 
AAGGGTTTCC ACTCCCTCAT CTTCGAAGTG GTCAGAGCAT CAGATGCAGG GCCATATGCA 
TGCGTGGCCA GGAACAGAGC TGGAGAAGCC ACCTTCACAG TGCAGCTGGA TGTTCTGGCA 
AAAGAGCATA AAAGAGCACC GATGTTTATC TTCAAACCAC AGAGTAAGAA AGTTTTTGAG 
GGAGAGACCG TGAAGCTGGA GTGCCAGATC TCAGCTATCC CTCCACCAAA GCTGTTCTGG 
AAAAGAAACA ATGAAATGGT CCAGTTCAAC ACTGATCGGA TAAGCTTATA TCATGACAAC 
GCCGGAAGAG TAACTTTACT GATAAAAGAT GTAAACAAGA AGGACGCTGG GTGGTATACA 
GTGTCAGCAG TCAATGAAGC TGGTGTGACC ACGTGTAACA CACGATTAGA TGTCACAGCC 
CGTCCAATCC AAACTCTCCC AGCTCCTAAG CAGCTGCGTG TTCGACCAAC ATTCAGCAAA 
TATTTAGCGC TCAATGGGAG AGGTTTGGAT GTGAAACAAG CTTTTAACCC TGAAGGAGAA 
TTTCAGCGCC TGGCAGCTCA ATCCGGACTC TACGAAAGTG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526196.1
CDS	174..1664
Translation	MFNYERPKHFIQPQNPCGSRLQPPGPEVSGFPSQTKQSSIVIQPRQCTEQRFSASSTVSSHITVSSSAYPAPQQLAGPNPGQKVTATYNQSPASFLSSILPSQPDYCNSKIPSTVDSNYQQSSVNQPVNAMSSQAANARPTPKTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKMARRLLGPQNAAAVFQAQNSDVQDSPQHNPEQARLHVPTSQVRSRSSSRAEANDQDAIQEKFYPPRFIQVPENMSIEEGRFCRMDFKVSGLPAPDVSWYLNGRPVQSDELHKMIVSEKGFHSLIFEVVRASDAGPYACVARNRAGEATFTVQLDVLAKEHKRAPMFIFKPQSKKVFEGETVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYHDNAGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPIQTLPAPKQLRVRPTFSKYLALNGRGLDVKQAFNPEGEFQRLAAQSGLYESEEL

Target ORF information:

RefSeq Version	XM_006526133.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myotilin (Myot), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526133.4

ORF Insert Sequence:

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CATAACCCAG AGCAAGCACG GCTGCACGTT 
CCCACATCAC AAGTAAGAAG TAGATCGTCC TCCAGAGCAG AGGCCAATGA TCAGGATGCC 
ATCCAGGAGA AGTTTTACCC ACCACGCTTC ATTCAAGTGC CAGAAAACAT GTCGATCGAA 
GAGGGGAGGT TCTGCCGAAT GGACTTTAAA GTAAGTGGAC TGCCAGCCCC TGATGTGTCA 
TGGTATCTAA ACGGGAGACC AGTGCAATCA GATGAGTTGC ACAAAATGAT AGTGTCTGAG 
AAGGGTTTCC ACTCCCTCAT CTTCGAAGTG GTCAGAGCAT CAGATGCAGG GCCATATGCA 
TGCGTGGCCA GGAACAGAGC TGGAGAAGCC ACCTTCACAG TGCAGCTGGA TGTTCTGGCA 
AAAGAGCATA AAAGAGCACC GATGTTTATC TTCAAACCAC AGAGTAAGAA AGTTTTTGAG 
GGAGAGACCG TGAAGCTGGA GTGCCAGATC TCAGCTATCC CTCCACCAAA GCTGTTCTGG 
AAAAGAAACA ATGAAATGGT CCAGTTCAAC ACTGATCGGA TAAGCTTATA TCATGACAAC 
GCCGGAAGAG TAACTTTACT GATAAAAGAT GTAAACAAGA AGGACGCTGG GTGGTATACA 
GTGTCAGCAG TCAATGAAGC TGGTGTGACC ACGTGTAACA CACGATTAGA TGTCACAGCC 
CGTCCAATCC AAACTCTCCC AGCTCCTAAG CAGCTGCGTG TTCGACCAAC ATTCAGCAAA 
TATTTAGCGC TCAATGGGAG AGGTTTGGAT GTGAAACAAG CTTTTAACCC TGAAGGAGAA 
TTTCAGCGCC TGGCAGCTCA ATCCGGACTC TACGAAAGTG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myot ( XM_006526132.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526195.1 Mus musculus myotilin (Myot), transcript variant X2, mRNA.

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CloneID	OMu54720
Clone ID Related Accession (Same CDS sequence)	XM_006526131.3 , XM_006526132.3 , XM_006526132.4 , XM_006526131.4
Accession Version	XM_006526132.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myotilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526132.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CAGCATAACC CAGAGCAAGC ACGGCTGCAC 
GTTCCCACAT CACAAGTAAG AAGTAGATCG TCCTCCAGAG CAGAGGCCAA TGATCAGGAT 
GCCATCCAGG AGAAGTTTTA CCCACCACGC TTCATTCAAG TGCCAGAAAA CATGTCGATC 
GAAGAGGGGA GGTTCTGCCG AATGGACTTT AAAGTAAGTG GACTGCCAGC CCCTGATGTG 
TCATGGTATC TAAACGGGAG ACCAGTGCAA TCAGATGAGT TGCACAAAAT GATAGTGTCT 
GAGAAGGGTT TCCACTCCCT CATCTTCGAA GTGGTCAGAG CATCAGATGC AGGGCCATAT 
GCATGCGTGG CCAGGAACAG AGCTGGAGAA GCCACCTTCA CAGTGCAGCT GGATGTTCTG 
GCAAAAGAGC ATAAAAGAGC ACCGATGTTT ATCTTCAAAC CACAGAGTAA GAAAGTTTTT 
GAGGGAGAGA CCGTGAAGCT GGAGTGCCAG ATCTCAGCTA TCCCTCCACC AAAGCTGTTC 
TGGAAAAGAA ACAATGAAAT GGTCCAGTTC AACACTGATC GGATAAGCTT ATATCATGAC 
AACGCCGGAA GAGTAACTTT ACTGATAAAA GATGTAAACA AGAAGGACGC TGGGTGGTAT 
ACAGTGTCAG CAGTCAATGA AGCTGGTGTG ACCACGTGTA ACACACGATT AGATGTCACA 
GCCCGTCCAA TCCAAACTCT CCCAGCTCCT AAGCAGCTGC GTGTTCGACC AACATTCAGC 
AAATATTTAG CGCTCAATGG GAGAGGTTTG GATGTGAAAC AAGCTTTTAA CCCTGAAGGA 
GAATTTCAGC GCCTGGCAGC TCAATCCGGA CTCTACGAAA GTGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526195.1
CDS	174..1667
Translation	MFNYERPKHFIQPQNPCGSRLQPPGPEVSGFPSQTKQSSIVIQPRQCTEQRFSASSTVSSHITVSSSAYPAPQQLAGPNPGQKVTATYNQSPASFLSSILPSQPDYCNSKIPSTVDSNYQQSSVNQPVNAMSSQAANARPTPKTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKMARRLLGPQNAAAVFQAQNSDVQDSPQQHNPEQARLHVPTSQVRSRSSSRAEANDQDAIQEKFYPPRFIQVPENMSIEEGRFCRMDFKVSGLPAPDVSWYLNGRPVQSDELHKMIVSEKGFHSLIFEVVRASDAGPYACVARNRAGEATFTVQLDVLAKEHKRAPMFIFKPQSKKVFEGETVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYHDNAGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPIQTLPAPKQLRVRPTFSKYLALNGRGLDVKQAFNPEGEFQRLAAQSGLYESEEL

Target ORF information:

RefSeq Version	XM_006526132.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myotilin (Myot), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526132.4

ORF Insert Sequence:

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CAGCATAACC CAGAGCAAGC ACGGCTGCAC 
GTTCCCACAT CACAAGTAAG AAGTAGATCG TCCTCCAGAG CAGAGGCCAA TGATCAGGAT 
GCCATCCAGG AGAAGTTTTA CCCACCACGC TTCATTCAAG TGCCAGAAAA CATGTCGATC 
GAAGAGGGGA GGTTCTGCCG AATGGACTTT AAAGTAAGTG GACTGCCAGC CCCTGATGTG 
TCATGGTATC TAAACGGGAG ACCAGTGCAA TCAGATGAGT TGCACAAAAT GATAGTGTCT 
GAGAAGGGTT TCCACTCCCT CATCTTCGAA GTGGTCAGAG CATCAGATGC AGGGCCATAT 
GCATGCGTGG CCAGGAACAG AGCTGGAGAA GCCACCTTCA CAGTGCAGCT GGATGTTCTG 
GCAAAAGAGC ATAAAAGAGC ACCGATGTTT ATCTTCAAAC CACAGAGTAA GAAAGTTTTT 
GAGGGAGAGA CCGTGAAGCT GGAGTGCCAG ATCTCAGCTA TCCCTCCACC AAAGCTGTTC 
TGGAAAAGAA ACAATGAAAT GGTCCAGTTC AACACTGATC GGATAAGCTT ATATCATGAC 
AACGCCGGAA GAGTAACTTT ACTGATAAAA GATGTAAACA AGAAGGACGC TGGGTGGTAT 
ACAGTGTCAG CAGTCAATGA AGCTGGTGTG ACCACGTGTA ACACACGATT AGATGTCACA 
GCCCGTCCAA TCCAAACTCT CCCAGCTCCT AAGCAGCTGC GTGTTCGACC AACATTCAGC 
AAATATTTAG CGCTCAATGG GAGAGGTTTG GATGTGAAAC AAGCTTTTAA CCCTGAAGGA 
GAATTTCAGC GCCTGGCAGC TCAATCCGGA CTCTACGAAA GTGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myot ( XM_006526131.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526194.1 Mus musculus myotilin (Myot), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54720
Clone ID Related Accession (Same CDS sequence)	XM_006526131.3 , XM_006526132.3 , XM_006526132.4 , XM_006526131.4
Accession Version	XM_006526131.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myotilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526131.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CAGCATAACC CAGAGCAAGC ACGGCTGCAC 
GTTCCCACAT CACAAGTAAG AAGTAGATCG TCCTCCAGAG CAGAGGCCAA TGATCAGGAT 
GCCATCCAGG AGAAGTTTTA CCCACCACGC TTCATTCAAG TGCCAGAAAA CATGTCGATC 
GAAGAGGGGA GGTTCTGCCG AATGGACTTT AAAGTAAGTG GACTGCCAGC CCCTGATGTG 
TCATGGTATC TAAACGGGAG ACCAGTGCAA TCAGATGAGT TGCACAAAAT GATAGTGTCT 
GAGAAGGGTT TCCACTCCCT CATCTTCGAA GTGGTCAGAG CATCAGATGC AGGGCCATAT 
GCATGCGTGG CCAGGAACAG AGCTGGAGAA GCCACCTTCA CAGTGCAGCT GGATGTTCTG 
GCAAAAGAGC ATAAAAGAGC ACCGATGTTT ATCTTCAAAC CACAGAGTAA GAAAGTTTTT 
GAGGGAGAGA CCGTGAAGCT GGAGTGCCAG ATCTCAGCTA TCCCTCCACC AAAGCTGTTC 
TGGAAAAGAA ACAATGAAAT GGTCCAGTTC AACACTGATC GGATAAGCTT ATATCATGAC 
AACGCCGGAA GAGTAACTTT ACTGATAAAA GATGTAAACA AGAAGGACGC TGGGTGGTAT 
ACAGTGTCAG CAGTCAATGA AGCTGGTGTG ACCACGTGTA ACACACGATT AGATGTCACA 
GCCCGTCCAA TCCAAACTCT CCCAGCTCCT AAGCAGCTGC GTGTTCGACC AACATTCAGC 
AAATATTTAG CGCTCAATGG GAGAGGTTTG GATGTGAAAC AAGCTTTTAA CCCTGAAGGA 
GAATTTCAGC GCCTGGCAGC TCAATCCGGA CTCTACGAAA GTGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526194.1
CDS	296..1789
Translation	MFNYERPKHFIQPQNPCGSRLQPPGPEVSGFPSQTKQSSIVIQPRQCTEQRFSASSTVSSHITVSSSAYPAPQQLAGPNPGQKVTATYNQSPASFLSSILPSQPDYCNSKIPSTVDSNYQQSSVNQPVNAMSSQAANARPTPKTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKMARRLLGPQNAAAVFQAQNSDVQDSPQQHNPEQARLHVPTSQVRSRSSSRAEANDQDAIQEKFYPPRFIQVPENMSIEEGRFCRMDFKVSGLPAPDVSWYLNGRPVQSDELHKMIVSEKGFHSLIFEVVRASDAGPYACVARNRAGEATFTVQLDVLAKEHKRAPMFIFKPQSKKVFEGETVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYHDNAGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPIQTLPAPKQLRVRPTFSKYLALNGRGLDVKQAFNPEGEFQRLAAQSGLYESEEL

Target ORF information:

RefSeq Version	XM_006526131.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myotilin (Myot), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526131.4

ORF Insert Sequence:

ATGTTTAACT ACGAACGTCC AAAACACTTC ATCCAGCCGC AAAATCCGTG TGGCTCCAGA 
CTGCAGCCTC CAGGACCGGA AGTCTCCGGC TTCCCGAGCC AGACCAAACA GTCTTCCATC 
GTCATACAGC CTCGCCAGTG CACAGAGCAG AGATTTTCTG CCTCCTCCAC AGTGAGCTCT 
CACATCACCG TGTCTTCCTC TGCTTATCCT GCTCCCCAGC AGCTTGCTGG CCCCAACCCC 
GGCCAAAAGG TTACTGCTAC CTATAACCAG TCCCCAGCCA GCTTCCTCAG TTCTATATTA 
CCGTCTCAAC CCGATTACTG TAACAGTAAA ATCCCATCCA CTGTGGACTC CAACTATCAA 
CAATCCTCAG TTAACCAACC TGTAAATGCC ATGTCATCCC AAGCTGCAAA TGCTAGGCCC 
ACTCCAAAGA CTCCTGACCA TGAGATCCAA GGATCGAAAG AAGCGTTGAT TCAAGATTTG 
GAAAGAAAGC TGAAATGCAA AGACACCCTT CTTCATAATG GAAATCAAAG ACTGACCTAC 
GAGGAGAAGA TGGCTCGCAG GTTGTTAGGA CCGCAGAATG CTGCTGCTGT GTTTCAGGCG 
CAAAACAGTG ATGTCCAAGA TTCACCACAG CAGCATAACC CAGAGCAAGC ACGGCTGCAC 
GTTCCCACAT CACAAGTAAG AAGTAGATCG TCCTCCAGAG CAGAGGCCAA TGATCAGGAT 
GCCATCCAGG AGAAGTTTTA CCCACCACGC TTCATTCAAG TGCCAGAAAA CATGTCGATC 
GAAGAGGGGA GGTTCTGCCG AATGGACTTT AAAGTAAGTG GACTGCCAGC CCCTGATGTG 
TCATGGTATC TAAACGGGAG ACCAGTGCAA TCAGATGAGT TGCACAAAAT GATAGTGTCT 
GAGAAGGGTT TCCACTCCCT CATCTTCGAA GTGGTCAGAG CATCAGATGC AGGGCCATAT 
GCATGCGTGG CCAGGAACAG AGCTGGAGAA GCCACCTTCA CAGTGCAGCT GGATGTTCTG 
GCAAAAGAGC ATAAAAGAGC ACCGATGTTT ATCTTCAAAC CACAGAGTAA GAAAGTTTTT 
GAGGGAGAGA CCGTGAAGCT GGAGTGCCAG ATCTCAGCTA TCCCTCCACC AAAGCTGTTC 
TGGAAAAGAA ACAATGAAAT GGTCCAGTTC AACACTGATC GGATAAGCTT ATATCATGAC 
AACGCCGGAA GAGTAACTTT ACTGATAAAA GATGTAAACA AGAAGGACGC TGGGTGGTAT 
ACAGTGTCAG CAGTCAATGA AGCTGGTGTG ACCACGTGTA ACACACGATT AGATGTCACA 
GCCCGTCCAA TCCAAACTCT CCCAGCTCCT AAGCAGCTGC GTGTTCGACC AACATTCAGC 
AAATATTTAG CGCTCAATGG GAGAGGTTTG GATGTGAAAC AAGCTTTTAA CCCTGAAGGA 
GAATTTCAGC GCCTGGCAGC TCAATCCGGA CTCTACGAAA GTGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Abnormal Mammary Development in 129:STAT1-Null Mice is Stroma-Dependent.
PloS one10(6)e0129895(2015)
Chen JQ,Mori H,Cardiff RD,Trott JF,Hovey RC,Hubbard NE,Engelberg JA,Tepper CG,Willis BJ,Khan IH,Ravindran RK,Chan SR,Schreiber RD,Borowsky AD

TAF7L modulates brown adipose tissue formation.
eLife3(2014 May)
Zhou H,Wan B,Grubisic I,Kaplan T,Tjian R

A class III PDZ binding motif in the myotilin and FATZ families binds enigma family proteins: a common link for Z-disc myopathies.
Molecular and cellular biology29(3)822-34(2009 Feb)
von Nandelstadh P,Ismail M,Gardin C,Suila H,Zara I,Belgrano A,Valle G,Carpen O,Faulkner G

Maintenance of muscle mass, fiber size, and contractile function in mice lacking the Z-disc protein myotilin.
Upsala journal of medical sciences114(4)235-41(2009)
Ochala J,Carp?n O,Larsson L

Myotilin overexpression enhances myopathology in the LGMD1A mouse model.
Muscle & nerve37(5)663-7(2008 May)
Garvey SM,Liu Y,Miller SE,Hauser MA

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Myot cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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