Eral1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eral1
Entrez Gene ID	57837
Full Name	Era (G-protein)-like 1 (E. coli)
Synonyms	2610524P08Rik,9130407C09Rik,AU019798,Era,M-ERA,MERA-S,MERA-W
General protein information	Preferred Names Era (G-protein)-like 1 (E. coli) Names GTPase Era, mitochondrial CEGA ERA-W ERA-like GTPase ERA-like protein 1 GTP-binding protein era homolog conserved ERA-like GTPase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_022313.2	NP_071708.2	GTPase Era, mitochondrial

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ERAL1	NP_001092492.1
Mus musculus (house mouse)	Eral1	NP_071708.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010622	XP_310115.3
Caenorhabditis elegans (roundworm)	E02H1.2	NP_496060.1
Pan troglodytes (chimpanzee)	ERAL1	XP_511365.3
	LOC716483	XP_001107234.2
Canis lupus familiaris (dog)	ERAL1	XP_537749.3
Drosophila melanogaster (fruit fly)	CG7488	NP_650248.1
Gallus gallus (chicken)	ERAL1	NP_989570.1
Danio rerio (zebrafish)	eral1	NP_001122219.1
Homo sapiens (human)	ERAL1	NP_005693.1
Rattus norvegicus (Norway rat)	Eral1	NP_001013247.1
Xenopus tropicalis (tropical clawed frog)	LOC100495069	XP_002938122.2

Gene Ontology
Bibliography

Related articles in PubMed

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.
Szczepanowska K, Maiti P, Kukat A, Hofsetz E, Nolte H, Senft K, Becker C, Ruzzenente B, Hornig-Do HT, Wibom R, Wiesner RJ, Kr?ger M, Trifunovic A
The EMBO journal35(23)2566-2583(2016 12)

Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways.
Thiagarajan RD, Georgas KM, Rumballe BA, Lesieur E, Chiu HS, Taylor D, Tang DT, Grimmond SM, Little MH
PloS one6(2)e17286(2011 Feb)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

Isolation and preliminary characterization of the human and mouse homologues of the bacterial cell cycle gene era.
Britton RA, Chen SM, Wallis D, Koeuth T, Powell BS, Shaffer LG, Largaespada D, Jenkins NA, Copeland NG, Court DL, Lupski JR
Genomics67(1)78-82(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors show that the defect in mitoribosomal assembly is a consequence of the accumulation of ERAL1, a putative 12S rRNA chaperone, and novel ClpXP substrate.
Title: CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.

The following Eral1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eral1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12583	NM_022313.2 Latest version!	Mus musculus Era (G-protein)-like 1 (E. coli) (Eral1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eral1 ( NM_022313.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_071708.2 Mus musculus Era (G-protein)-like 1 (E. coli) (Eral1), mRNA.

Price & Availability↑

CloneID	OMu12583
Clone ID Related Accession (Same CDS sequence)	NM_022313.2
Accession Version	NM_022313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Mus musculus(house mouse)
Product	GTPase Era, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY158323.1, AK012155.1 and AF190730.1. On Jan 16, 2008 this sequence version replaced NM_022313.1. ##Evidence-Data-START## Transcript exon combination :: AF190730.1, BC068271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCC CCAGGCGGTA CTGCGCTGGG CTCGTTCGGG CCTTGTTAGG GGCCCGACAG 
GTGGGTTCGC ATGCAGGGAG GGAATGGCTT GCTCCGCCCG GCTGTCTTCT AGGTAACCAG 
GCGAGGTGCG TGTCCTGTGT CGTGGGCTCC ACTTTCTCTG GTCCCCTCCT GGCCTCGGCC 
TCTAGTCGTT ATGGTCAGGA CTCCGCCTTG GACCGTATCC TTGGATTTTC TCAGCCTGAC 
AGTTCGTTGG TTCCGAGCGT CCCCGCGGTG TCGGTGCACA GAGATGAGCA GAATCTACTC 
TTGGTGCACA CTCCCGACAT GCCTGAGAAT CCACGGGTCC TCCGCGTGGT CCTCCTGGGA 
GCCCCAAATG CAGGGAAGTC AACACTTTCC AATCAGCTGC TGGGCCGTAA AGTGTTTCCT 
GTTTCAAAGA AGGTGCACAC CACTCGATGT CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAAGTGA TTCTGCTTGA CACACCTGGC ATCATCAGTC CTGTTAAACA GAAGAGGCAC 
CATCTGGAGC GCTCTTTGCT GGAAGATCCA TGGACGAGCA TGGAATCTGC TGATCTTGTT 
GTAGTTCTTG TGGATGTGTC AGACAAGTGG ACCAGGAGTC GGCTCAACCC CCAGGTGCTG 
CAGTGCTTGA CCAAGTTCTC TCAGGTCCCT AGCATCCTCG TCTTAAACAA GGTAGATTGC 
CTGAAGCAGA AGTCAGTTCT CCTGGAGCTG ACAGCAGCCC TCACTGAAGG TGTTGTCAAT 
GGCAAGAAGC TTAATATCAA GCAGGCCTTG CGCTCACGCT CCAGCACCCA CTGCCCTGGC 
CCTGAAACTG AGGGCCCAAA TGCACACTCA GTGAGGAACC CTCAGAGAAT TGGCTGGCCC 
TACTTCCAGG AGATCTTCAT GTTATCAGCA CTAAACAACA AGGATGTGAA CACATTGAAG 
CAATACCTTC TGACACAGGC CCAGCCAGGA CCCTGGGAGT TCCACAGTGG TGTGCTCACT 
AGCCAGACAC CCGAAGAGAT CTGTGCCAAT AAAATCAGAG AGAAGCTCTT AGAGTACCTG 
CCTGAGGAGG TGCCCTACGG TGTGCAGCAG AAGACAGTGA TATGGGAGGA AGGACCAAGT 
GGAGAGCTAG TGATCCAACA GAACCTTCTG GTGCCCAAAG AATCTCATGT GAGGATCCTG 
ATTGGTCAGA AGGGCCTCTT GATCTCCCAG ATTGCACAGG AGGTGGGCCG GGACCTCATG 
GACATCTTCC ACTGTGACGT TCTCATCCGC CTCTCTGTGA AACTCCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071708.2
CDS	25..1338
Translation	MAAPRRYCAGLVRALLGARQVGSHAGREWLAPPGCLLGNQARCVSCVVGSTFSGPLLASASSRYGQDSALDRILGFSQPDSSLVPSVPAVSVHRDEQNLLLVHTPDMPENPRVLRVVLLGAPNAGKSTLSNQLLGRKVFPVSKKVHTTRCQALGVITEKETQVILLDTPGIISPVKQKRHHLERSLLEDPWTSMESADLVVVLVDVSDKWTRSRLNPQVLQCLTKFSQVPSILVLNKVDCLKQKSVLLELTAALTEGVVNGKKLNIKQALRSRSSTHCPGPETEGPNAHSVRNPQRIGWPYFQEIFMLSALNNKDVNTLKQYLLTQAQPGPWEFHSGVLTSQTPEEICANKIREKLLEYLPEEVPYGVQQKTVIWEEGPSGELVIQQNLLVPKESHVRILIGQKGLLISQIAQEVGRDLMDIFHCDVLIRLSVKLLK

Target ORF information:

RefSeq Version	NM_022313.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Era (G-protein)-like 1 (E. coli) (Eral1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022313.2

ORF Insert Sequence:

ATGGCTGCCC CCAGGCGGTA CTGCGCTGGG CTCGTTCGGG CCTTGTTAGG GGCCCGACAG 
GTGGGTTCGC ATGCAGGGAG GGAATGGCTT GCTCCGCCCG GCTGTCTTCT AGGTAACCAG 
GCGAGGTGCG TGTCCTGTGT CGTGGGCTCC ACTTTCTCTG GTCCCCTCCT GGCCTCGGCC 
TCTAGTCGTT ATGGTCAGGA CTCCGCCTTG GACCGTATCC TTGGATTTTC TCAGCCTGAC 
AGTTCGTTGG TTCCGAGCGT CCCCGCGGTG TCGGTGCACA GAGATGAGCA GAATCTACTC 
TTGGTGCACA CTCCCGACAT GCCTGAGAAT CCACGGGTCC TCCGCGTGGT CCTCCTGGGA 
GCCCCAAATG CAGGGAAGTC AACACTTTCC AATCAGCTGC TGGGCCGTAA AGTGTTTCCT 
GTTTCAAAGA AGGTGCACAC CACTCGATGT CAAGCTCTGG GGGTCATCAC AGAGAAGGAG 
ACCCAAGTGA TTCTGCTTGA CACACCTGGC ATCATCAGTC CTGTTAAACA GAAGAGGCAC 
CATCTGGAGC GCTCTTTGCT GGAAGATCCA TGGACGAGCA TGGAATCTGC TGATCTTGTT 
GTAGTTCTTG TGGATGTGTC AGACAAGTGG ACCAGGAGTC GGCTCAACCC CCAGGTGCTG 
CAGTGCTTGA CCAAGTTCTC TCAGGTCCCT AGCATCCTCG TCTTAAACAA GGTAGATTGC 
CTGAAGCAGA AGTCAGTTCT CCTGGAGCTG ACAGCAGCCC TCACTGAAGG TGTTGTCAAT 
GGCAAGAAGC TTAATATCAA GCAGGCCTTG CGCTCACGCT CCAGCACCCA CTGCCCTGGC 
CCTGAAACTG AGGGCCCAAA TGCACACTCA GTGAGGAACC CTCAGAGAAT TGGCTGGCCC 
TACTTCCAGG AGATCTTCAT GTTATCAGCA CTAAACAACA AGGATGTGAA CACATTGAAG 
CAATACCTTC TGACACAGGC CCAGCCAGGA CCCTGGGAGT TCCACAGTGG TGTGCTCACT 
AGCCAGACAC CCGAAGAGAT CTGTGCCAAT AAAATCAGAG AGAAGCTCTT AGAGTACCTG 
CCTGAGGAGG TGCCCTACGG TGTGCAGCAG AAGACAGTGA TATGGGAGGA AGGACCAAGT 
GGAGAGCTAG TGATCCAACA GAACCTTCTG GTGCCCAAAG AATCTCATGT GAGGATCCTG 
ATTGGTCAGA AGGGCCTCTT GATCTCCCAG ATTGCACAGG AGGTGGGCCG GGACCTCATG 
GACATCTTCC ACTGTGACGT TCTCATCCGC CTCTCTGTGA AACTCCTCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.
The EMBO journal35(23)2566-2583(2016 12)
Szczepanowska K,Maiti P,Kukat A,Hofsetz E,Nolte H,Senft K,Becker C,Ruzzenente B,Hornig-Do HT,Wibom R,Wiesner RJ,Kr?ger M,Trifunovic A

Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways.
PloS one6(2)e17286(2011 Feb)
Thiagarajan RD,Georgas KM,Rumballe BA,Lesieur E,Chiu HS,Taylor D,Tang DT,Grimmond SM,Little MH

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

Isolation and preliminary characterization of the human and mouse homologues of the bacterial cell cycle gene era.
Genomics67(1)78-82(2000 Jul)
Britton RA,Chen SM,Wallis D,Koeuth T,Powell BS,Shaffer LG,Largaespada D,Jenkins NA,Copeland NG,Court DL,Lupski JR

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Eral1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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