Fhl5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fhl5
Entrez Gene ID	57756
Full Name	four and a half LIM domains 5
Synonyms	1700027G07Rik,Act
General protein information	Preferred Names four and a half LIM domains 5 Names four and a half LIM domains protein 5 FHL-5 LIM-only protein ACT activator of CREM in testis activator of cAMP-responsive element modulator in testis
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011250080.2	XP_011248382.1	four and a half LIM domains protein 5 isoform X2
XM_011250080.1	XP_011248382.1	four and a half LIM domains protein 5 isoform X1
XM_006538125.3	XP_006538188.1	four and a half LIM domains protein 5 isoform X1
XM_006538125.4	XP_006538188.1	four and a half LIM domains protein 5 isoform X1
NM_001355498.1	NP_001342427.1	four and a half LIM domains protein 5
NM_021318.3	NP_067293.1	four and a half LIM domains protein 5
NM_021318.4	NP_067293.1	four and a half LIM domains protein 5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Fhl5	NP_001013106.1
Gallus gallus (chicken)	FHL5	NP_001264652.1
	FHL5	XP_001100773.2
Canis lupus familiaris (dog)	FHL5	XP_532237.2
Pan troglodytes (chimpanzee)	FHL5	NP_001233299.1
Bos taurus (cattle)	FHL5	NP_001069847.1
Homo sapiens (human)	FHL5	NP_001164278.1
Mus musculus (house mouse)	Fhl5	NP_067293.1
Xenopus tropicalis (tropical clawed frog)	fhl5	NP_001116957.1

Gene Ontology
Bibliography

Related articles in PubMed

TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice.
Yabuta Y, Ohta H, Abe T, Kurimoto K, Chuma S, Saitou M
The Journal of cell biology192(5)781-95(2011 Mar)

Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
Wu H, Chen Y, Miao S, Zhang C, Zong S, Koide SS, Wang L
FEBS letters584(13)2807-15(2010 Jul)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.
Lardenois A, Chalmel F, Demougin P, Kotaja N, Sassone-Corsi P, Primig M
Reproductive biology and endocrinology : RB&E7133(2009 Nov)

GUDMAP: the genitourinary developmental molecular anatomy project.
McMahon AP, Aronow BJ, Davidson DR, Davies JA, Gaido KW, Grimmond S, Lessard JL, Little MH, Potter SS, Wilder EL, Zhang P,
Journal of the American Society of Nephrology : JASN19(4)667-71(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis.
Title: Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.

Fhl5 is not essential for testicular gene expression to a level detected by microarray-based measurements.
Title: Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.

Results indicate that numerous postmeiotic genes under CREM (cAMP-responsive element modulator) control require the coactivator function of ACT (activator of CREM) in testis.
Title: Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene.

ACT selectively associates with KIF17b, a kinesin highly expressed in male germ cells; The ACT-KIF17b interaction is restricted to specific stages of spermatogenesis and directly determines the intracellular localization of ACT
Title: CREM-dependent transcription in male germ cells controlled by a kinesin.

The following Fhl5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fhl5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12168	NM_001355498.1 Latest version!	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu12168	NM_021318.4 Latest version!	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu12168	XM_011250080.2 Latest version!	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu33069	XM_006538125.4 Latest version!	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu12168	XM_011250080.1	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu33069	XM_006538125.3	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu12168	NM_021318.3	Mus musculus four and a half LIM domains 5 (Fhl5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fhl5 ( XM_006538125.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538188.1 Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33069
Clone ID Related Accession (Same CDS sequence)	XM_006538125.3 , XM_006538125.4
Accession Version	XM_006538125.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC CTCTCTGGGA TAAAACTGAA ATGACAAGTA GTCAATTTGA TTGTCAATAC 
TGCACTTCAT CCCTGATTGG GAAGAAATAT GTACTCAAGG ATGATAATCT ATACTGCATC 
TCCTGCTACG ATCGTATCTT TTCTAACTAT TGTGAGCAGT GTAAAGAACC AATTGAATCA 
GATTCTAAGG ATCTTTGCTA CAAAAACCGT CACTGGCATG AAGGATGCTT CAGGTGCAAC 
AAATGCCATC ACTCTTTGGT GGAAAAGCCT TTCGTTGCCA AGGATGATCG CCTGCTGTGC 
ACAGACTGCT ATTCCAACGA GTGTTCCTCC AAGTGCTTCC ACTGCAAGAG AACCATCATG 
CCAGGTTCTC GGAAAATGGA ATTTAAGGGC AATTACTGGC ATGAAACCTG CTTTGTGTGT 
GAGCACTGCC GACAGCCAAT AGGAACCAAG CCTTTGATCT CCAAAGAGAG TGGCAATTAT 
TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT CATTACTGCA ACTTCTGTAA GAAGGTGATA 
ACTTCCGGTG GGATAACCTT CCGTGATCAG ATATGGCATA AAGAGTGTTT TCTGTGCAGC 
GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA TTTATGTCAA AGGATGATTT CCCATTCTGC 
CTGGATTGCT ACAACCATCT TTATGCTAAA AAGTGTGCAG CCTGCACCAA ACCCATCACT 
GGCCTCAGAG GTGCCAAGTT CATCTGCTTT CAAGACCGCC AGTGGCACAG TGAGTGCTTC 
AACTGCGGAA AGTGCTCGGT CTCCTTGGTG GGTGAAGGAT TCTTGACCCA TAACATGGAA 
ATCTTATGCC GCAAGTGTGG CTCCGGGGCA GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538188.1
CDS	387..1271
Translation	MELPLWDKTEMTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA

Target ORF information:

RefSeq Version	XM_006538125.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538125.3

ORF Insert Sequence:

ATGGAATTGC CTCTCTGGGA TAAAACTGAA ATGACAAGTA GTCAATTTGA TTGTCAATAC 
TGCACTTCAT CCCTGATTGG GAAGAAATAT GTACTCAAGG ATGATAATCT ATACTGCATC 
TCCTGCTACG ATCGTATCTT TTCTAACTAT TGTGAGCAGT GTAAAGAACC AATTGAATCA 
GATTCTAAGG ATCTTTGCTA CAAAAACCGT CACTGGCATG AAGGATGCTT CAGGTGCAAC 
AAATGCCATC ACTCTTTGGT GGAAAAGCCT TTCGTTGCCA AGGATGATCG CCTGCTGTGC 
ACAGACTGCT ATTCCAACGA GTGTTCCTCC AAGTGCTTCC ACTGCAAGAG AACCATCATG 
CCAGGTTCTC GGAAAATGGA ATTTAAGGGC AATTACTGGC ATGAAACCTG CTTTGTGTGT 
GAGCACTGCC GACAGCCAAT AGGAACCAAG CCTTTGATCT CCAAAGAGAG TGGCAATTAT 
TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT CATTACTGCA ACTTCTGTAA GAAGGTGATA 
ACTTCCGGTG GGATAACCTT CCGTGATCAG ATATGGCATA AAGAGTGTTT TCTGTGCAGC 
GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA TTTATGTCAA AGGATGATTT CCCATTCTGC 
CTGGATTGCT ACAACCATCT TTATGCTAAA AAGTGTGCAG CCTGCACCAA ACCCATCACT 
GGCCTCAGAG GTGCCAAGTT CATCTGCTTT CAAGACCGCC AGTGGCACAG TGAGTGCTTC 
AACTGCGGAA AGTGCTCGGT CTCCTTGGTG GGTGAAGGAT TCTTGACCCA TAACATGGAA 
ATCTTATGCC GCAAGTGTGG CTCCGGGGCA GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl5 ( NM_001355498.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342427.1 Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu12168
Clone ID Related Accession (Same CDS sequence)	XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4
Accession Version	NM_001355498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL935267.13, AK006414.1 and AV205329.1. On Sep 19, 2017 this sequence version replaced XM_011250080.2. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.192388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342427.1
CDS	77..931
Translation	MTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA

Target ORF information:

RefSeq Version	NM_001355498.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355498.1

ORF Insert Sequence:

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl5 ( NM_021318.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067293.1 Mus musculus four and a half LIM domains 5 (Fhl5), mRNA.

Price & Availability↑

CloneID	OMu12168
Clone ID Related Accession (Same CDS sequence)	XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4
Accession Version	NM_021318.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	four and a half LIM domains protein 5
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048473.1. On Apr 5, 2007 this sequence version replaced NM_021318.2. ##Evidence-Data-START## Transcript exon combination :: BC048473.1, AK006414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067293.1
CDS	182..1036
Translation	MTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA

Target ORF information:

RefSeq Version	NM_021318.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus four and a half LIM domains 5 (Fhl5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021318.3

ORF Insert Sequence:

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl5 ( NM_021318.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067293.1 Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12168
Clone ID Related Accession (Same CDS sequence)	XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4
Accession Version	NM_021318.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL935267.13, AK006414.1 and AV205329.1. On Sep 19, 2017 this sequence version replaced NM_021318.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC048473.1, AK006414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067293.1
CDS	204..1058
Translation	MTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA

Target ORF information:

RefSeq Version	NM_021318.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021318.4

ORF Insert Sequence:

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl5 ( XM_011250080.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248382.1 Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X2, mRNA.

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CloneID	OMu12168
Clone ID Related Accession (Same CDS sequence)	XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4
Accession Version	XM_011250080.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	four and a half LIM domains protein 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248382.1
CDS	101..955
Translation	MTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA

Target ORF information:

RefSeq Version	XM_011250080.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250080.2

ORF Insert Sequence:

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl5 ( XM_011250080.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248382.1 Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu12168
Clone ID Related Accession (Same CDS sequence)	XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4
Accession Version	XM_011250080.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	four and a half LIM domains protein 5 isoform X1
Comment

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248382.1
Translation	MTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA

Target ORF information:

RefSeq Version	XM_011250080.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250080.1

ORF Insert Sequence:

ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT 
GTACTCAAGG ATGATAATCT ATACTGCATC TCCTGCTACG ATCGTATCTT TTCTAACTAT 
TGTGAGCAGT GTAAAGAACC AATTGAATCA GATTCTAAGG ATCTTTGCTA CAAAAACCGT 
CACTGGCATG AAGGATGCTT CAGGTGCAAC AAATGCCATC ACTCTTTGGT GGAAAAGCCT 
TTCGTTGCCA AGGATGATCG CCTGCTGTGC ACAGACTGCT ATTCCAACGA GTGTTCCTCC 
AAGTGCTTCC ACTGCAAGAG AACCATCATG CCAGGTTCTC GGAAAATGGA ATTTAAGGGC 
AATTACTGGC ATGAAACCTG CTTTGTGTGT GAGCACTGCC GACAGCCAAT AGGAACCAAG 
CCTTTGATCT CCAAAGAGAG TGGCAATTAT TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT 
CATTACTGCA ACTTCTGTAA GAAGGTGATA ACTTCCGGTG GGATAACCTT CCGTGATCAG 
ATATGGCATA AAGAGTGTTT TCTGTGCAGC GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA 
TTTATGTCAA AGGATGATTT CCCATTCTGC CTGGATTGCT ACAACCATCT TTATGCTAAA 
AAGTGTGCAG CCTGCACCAA ACCCATCACT GGCCTCAGAG GTGCCAAGTT CATCTGCTTT 
CAAGACCGCC AGTGGCACAG TGAGTGCTTC AACTGCGGAA AGTGCTCGGT CTCCTTGGTG 
GGTGAAGGAT TCTTGACCCA TAACATGGAA ATCTTATGCC GCAAGTGTGG CTCCGGGGCA 
GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl5 ( XM_006538125.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538188.1 Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33069
Clone ID Related Accession (Same CDS sequence)	XM_006538125.3 , XM_006538125.4
Accession Version	XM_006538125.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538125.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC CTCTCTGGGA TAAAACTGAA ATGACAAGTA GTCAATTTGA TTGTCAATAC 
TGCACTTCAT CCCTGATTGG GAAGAAATAT GTACTCAAGG ATGATAATCT ATACTGCATC 
TCCTGCTACG ATCGTATCTT TTCTAACTAT TGTGAGCAGT GTAAAGAACC AATTGAATCA 
GATTCTAAGG ATCTTTGCTA CAAAAACCGT CACTGGCATG AAGGATGCTT CAGGTGCAAC 
AAATGCCATC ACTCTTTGGT GGAAAAGCCT TTCGTTGCCA AGGATGATCG CCTGCTGTGC 
ACAGACTGCT ATTCCAACGA GTGTTCCTCC AAGTGCTTCC ACTGCAAGAG AACCATCATG 
CCAGGTTCTC GGAAAATGGA ATTTAAGGGC AATTACTGGC ATGAAACCTG CTTTGTGTGT 
GAGCACTGCC GACAGCCAAT AGGAACCAAG CCTTTGATCT CCAAAGAGAG TGGCAATTAT 
TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT CATTACTGCA ACTTCTGTAA GAAGGTGATA 
ACTTCCGGTG GGATAACCTT CCGTGATCAG ATATGGCATA AAGAGTGTTT TCTGTGCAGC 
GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA TTTATGTCAA AGGATGATTT CCCATTCTGC 
CTGGATTGCT ACAACCATCT TTATGCTAAA AAGTGTGCAG CCTGCACCAA ACCCATCACT 
GGCCTCAGAG GTGCCAAGTT CATCTGCTTT CAAGACCGCC AGTGGCACAG TGAGTGCTTC 
AACTGCGGAA AGTGCTCGGT CTCCTTGGTG GGTGAAGGAT TCTTGACCCA TAACATGGAA 
ATCTTATGCC GCAAGTGTGG CTCCGGGGCA GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538188.1
CDS	377..1261
Translation	MELPLWDKTEMTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCGSGADTDA

Target ORF information:

RefSeq Version	XM_006538125.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 5 (Fhl5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538125.4

ORF Insert Sequence:

ATGGAATTGC CTCTCTGGGA TAAAACTGAA ATGACAAGTA GTCAATTTGA TTGTCAATAC 
TGCACTTCAT CCCTGATTGG GAAGAAATAT GTACTCAAGG ATGATAATCT ATACTGCATC 
TCCTGCTACG ATCGTATCTT TTCTAACTAT TGTGAGCAGT GTAAAGAACC AATTGAATCA 
GATTCTAAGG ATCTTTGCTA CAAAAACCGT CACTGGCATG AAGGATGCTT CAGGTGCAAC 
AAATGCCATC ACTCTTTGGT GGAAAAGCCT TTCGTTGCCA AGGATGATCG CCTGCTGTGC 
ACAGACTGCT ATTCCAACGA GTGTTCCTCC AAGTGCTTCC ACTGCAAGAG AACCATCATG 
CCAGGTTCTC GGAAAATGGA ATTTAAGGGC AATTACTGGC ATGAAACCTG CTTTGTGTGT 
GAGCACTGCC GACAGCCAAT AGGAACCAAG CCTTTGATCT CCAAAGAGAG TGGCAATTAT 
TGTGTGCCAT GTTTTGAGAA GGAGTTTGCT CATTACTGCA ACTTCTGTAA GAAGGTGATA 
ACTTCCGGTG GGATAACCTT CCGTGATCAG ATATGGCATA AAGAGTGTTT TCTGTGCAGC 
GGCTGCAGGA AAGAGCTTTA TGAGGAGGCA TTTATGTCAA AGGATGATTT CCCATTCTGC 
CTGGATTGCT ACAACCATCT TTATGCTAAA AAGTGTGCAG CCTGCACCAA ACCCATCACT 
GGCCTCAGAG GTGCCAAGTT CATCTGCTTT CAAGACCGCC AGTGGCACAG TGAGTGCTTC 
AACTGCGGAA AGTGCTCGGT CTCCTTGGTG GGTGAAGGAT TCTTGACCCA TAACATGGAA 
ATCTTATGCC GCAAGTGTGG CTCCGGGGCA GACACTGACG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice.
The Journal of cell biology192(5)781-95(2011 Mar)
Yabuta Y,Ohta H,Abe T,Kurimoto K,Chuma S,Saitou M

Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
FEBS letters584(13)2807-15(2010 Jul)
Wu H,Chen Y,Miao S,Zhang C,Zong S,Koide SS,Wang L

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.
Reproductive biology and endocrinology : RB&E7133(2009 Nov)
Lardenois A,Chalmel F,Demougin P,Kotaja N,Sassone-Corsi P,Primig M

GUDMAP: the genitourinary developmental molecular anatomy project.
Journal of the American Society of Nephrology : JASN19(4)667-71(2008 Apr)
McMahon AP,Aronow BJ,Davidson DR,Davies JA,Gaido KW,Grimmond S,Lessard JL,Little MH,Potter SS,Wilder EL,Zhang P,

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Fhl5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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