Ehd3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ehd3
Entrez Gene ID	57440
Full Name	EH-domain containing 3
Synonyms	Ehd2
General protein information	Preferred Names EH-domain containing 3 Names EH domain-containing protein 3 EH-domain containing protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 E2\|17 45.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_020578.3	NP_065603.2	EH domain-containing protein 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ehd3	NP_065603.2
Arabidopsis thaliana (thale cress)	EHD2	NP_974517.1
Homo sapiens (human)	EHD3	NP_055415.1
Pan troglodytes (chimpanzee)	EHD3	XP_515386.2
Oryza sativa (rice)	Os06g0687800	NP_001058410.1
	EHD3	XP_001105262.1
Canis lupus familiaris (dog)	EHD3	XP_853995.2
Bos taurus (cattle)	EHD3	NP_001091473.1
Rattus norvegicus (Norway rat)	Ehd3	NP_620245.2
Oryza sativa (rice)	Os02g0158100	NP_001045954.1
Gallus gallus (chicken)	EHD3	NP_001026211.1
Danio rerio (zebrafish)	ehd3	NP_001038469.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of the EHD Family of Endocytic Recycling Regulators for TCR Recycling and T Cell Function.
Iseka FM, Goetz BT, Mushtaq I, An W, Cypher LR, Bielecki TA, Tom EC, Arya P, Bhattacharyya S, Storck MD, Semerad CL, Talmadge JE, Mosley RL, Band V, Band H
Journal of immunology (Baltimore, Md. : 1950)200(2)483-499(2018 Jan)

Phosphatidic acid induces EHD3-containing membrane tubulation and is required for receptor recycling.
Henmi Y, Oe N, Kono N, Taguchi T, Takei K, Tanabe K
Experimental cell research342(1)1-10(2016 Mar)

Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.
Bhattacharyya S, Rainey MA, Arya P, Dutta S, George M, Storck MD, McComb RD, Muirhead D, Todd GL, Gould K, Datta K, Gelineau-van Waes J, Band V, Band H
Scientific reports620727(2016 Feb)

The endocytic recycling regulatory protein EHD1 Is required for ocular lens development.
Arya P, Rainey MA, Bhattacharyya S, Mohapatra BC, George M, Kuracha MR, Storck MD, Band V, Govindarajan V, Band H
Developmental biology408(1)41-55(2015 Dec)

SUMOylation of EHD3 Modulates Tubulation of the Endocytic Recycling Compartment.
Cabasso O, Pekar O, Horowitz M
PloS one10(7)e0134053(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EHD3 and phosphatidic acid cooperatively regulate membrane deformation and trafficking from early endosomes.
Title: Phosphatidic acid induces EHD3-containing membrane tubulation and is required for receptor recycling.

CaV3.1 and CaV3.2 are substrates for EHD3-dependent protein trafficking in heart
Title: Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria.

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
Title: Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

EHD3 is a critical component of protein trafficking in heart and is essential for the proper membrane targeting of select cellular proteins that maintain excitability.
Title: EHD3-dependent endosome pathway regulates cardiac membrane excitability and physiology.

EHD-mediated endocytic traffic of key surface receptors such as VEGFR2 is essential for physiological control of glomerular function.
Title: Renal thrombotic microangiopathy in mice with combined deletion of endocytic recycling regulators EHD3 and EHD4.

The following Ehd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ehd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12112	NM_020578.3 Latest version!	Mus musculus EH-domain containing 3 (Ehd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ehd3 ( NM_020578.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_065603.2 Mus musculus EH-domain containing 3 (Ehd3), mRNA.

Price & Availability↑

CloneID	OMu12112
Clone ID Related Accession (Same CDS sequence)	NM_020578.3
Accession Version	NM_020578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	EH domain-containing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK141265.1 and AA510832.1. On Dec 5, 2008 this sequence version replaced NM_020578.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK141265.1, AF155883.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTTCAGTT GGCTGGGTAA CGATGATCGC CGCAAGAAGG ACCCTGAGGT CTTCCAGACG 
GTGAGCGATG GACTCAAAAA ACTCTACAAG ACCAAGCTGC TGCCTCTGGA AGAGTATTAC 
CGCTTCCACG AGTTCCACTC GCCCGCCCTG GAAGATGCTG ATTTCGACAA CAAGCCCATG 
GTCCTGTTGG TGGGCCAGTA CTCTACCGGC AAGACCACCT TCATCAGGTA CCTGCTGGAA 
CAGGATTTTC CAGGCATGAG GATTGGGCCT GAGCCGACCA CTGATTCCTT CATAGCAGTG 
ATGCAGGGAG ATGTGGAGGG GATCATTCCC GGGAATGCCT TGGTGGTGGA TCCAAAGAAA 
CCCTTCAGAA AGCTCAACGC CTTTGGCAAC GCCTTCCTGA ACAGGTTTGT GTGTGCCCAG 
CTGCCCAACG CCGTGCTTGA AAGTATCAGT GTGATCGACA CACCGGGGAT CCTCTCTGGG 
GAGAAGCAGA GGATCAGCCG AGGGTATGAT TTTGCTGCGG TCCTCGAATG GTTTGCTGAG 
CGGGTGGACC GAATTATCCT ACTCTTCGAC GCCCACAAGC TGGACATCTC CGATGAGTTC 
TCAGAAGTCA TCAAGGCTCT CAAGAACCAT GAGGACAAGA TGCGCGTAGT GTTGAACAAA 
GCTGACCAGA TCGAGACCCA GCAGCTGATG CGAGTATACG GAGCCCTCAT GTGGTCCCTG 
GGGAAGATCG TGAACACCCC CGAGGTGATC CGGGTCTACA TTGGCTCCTT CTGGTCCCAC 
CCACTCCTCA TTCCCGACAA CCGGAAGCTC TTTGAAGCTG AAGAGCAAGA CTTGTTCAGA 
GACATTCAGA GTCTACCCCG TAATGCTGCT CTTCGAAAGC TCAACGATCT CATCAAGAGA 
GCCCGGCTGG CCAAGGTCCA CGCCTACATC ATCAGCTCCT TGAAGAAGGA GATGCCCTCA 
GTGTTTGGGA AGGACACCAA AAAGAAAGAA CTGGTGAACA ACCTGGCTGA GATCTATGGC 
CGGATTGAGC GAGAACACCA GATCTCCCCT GGAGACTTCC CCAACCTGAA GAGGATGCAG 
GACCAGCTGC AGGCCCAGGA CTTCAGCAAA TTCCAGCCAC TGAAGAGCAA GCTGCTGGAA 
GTGGTTGATG ATATGCTGGC TCATGACATT GCCCAGCTCA TGGTGCTGGT GCGCCAGGAA 
GAGACCCAAC GGCCTGTCCA GATGGTGAAG GGCGGAGCAT TTGAGGGAAC CTTACAAGGC 
CCCTTCGGGC ACGGCTATGG AGAGGGAGCT GGGGAGGGCA TCGATGATGC CGAGTGGGTG 
GTGGCGCGGG ACAAGCCTAT GTATGATGAG ATCTTCTACA CCTTATCCCC AGTGGATGGC 
AAGATCACAG GTGCCAACGC CAAGAAGGAG ATGGTGCGCT CCAAGTTGCC CAACAGCGTG 
CTGGGCAAGA TCTGGAAGCT AGCCGACATT GACAAGGATG GCATGTTGGA TGACGAGGAG 
TTTGCCCTGG CCAACCACCT TATCAAAGTC AAGCTAGAGG GGCATGAGCT GCCCAGTGAG 
CTACCTGCCC ACCTCCTCCC TCCATCTAAG AGGAAAGTAT CAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065603.2
CDS	403..2010
Translation	MFSWLGNDDRRKKDPEVFQTVSDGLKKLYKTKLLPLEEYYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDVEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNAVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDTKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEETQRPVQMVKGGAFEGTLQGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDEEFALANHLIKVKLEGHELPSELPAHLLPPSKRKVSE

Target ORF information:

RefSeq Version	NM_020578.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EH-domain containing 3 (Ehd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020578.3

ORF Insert Sequence:

ATGTTCAGTT GGCTGGGTAA CGATGATCGC CGCAAGAAGG ACCCTGAGGT CTTCCAGACG 
GTGAGCGATG GACTCAAAAA ACTCTACAAG ACCAAGCTGC TGCCTCTGGA AGAGTATTAC 
CGCTTCCACG AGTTCCACTC GCCCGCCCTG GAAGATGCTG ATTTCGACAA CAAGCCCATG 
GTCCTGTTGG TGGGCCAGTA CTCTACCGGC AAGACCACCT TCATCAGGTA CCTGCTGGAA 
CAGGATTTTC CAGGCATGAG GATTGGGCCT GAGCCGACCA CTGATTCCTT CATAGCAGTG 
ATGCAGGGAG ATGTGGAGGG GATCATTCCC GGGAATGCCT TGGTGGTGGA TCCAAAGAAA 
CCCTTCAGAA AGCTCAACGC CTTTGGCAAC GCCTTCCTGA ACAGGTTTGT GTGTGCCCAG 
CTGCCCAACG CCGTGCTTGA AAGTATCAGT GTGATCGACA CACCGGGGAT CCTCTCTGGG 
GAGAAGCAGA GGATCAGCCG AGGGTATGAT TTTGCTGCGG TCCTCGAATG GTTTGCTGAG 
CGGGTGGACC GAATTATCCT ACTCTTCGAC GCCCACAAGC TGGACATCTC CGATGAGTTC 
TCAGAAGTCA TCAAGGCTCT CAAGAACCAT GAGGACAAGA TGCGCGTAGT GTTGAACAAA 
GCTGACCAGA TCGAGACCCA GCAGCTGATG CGAGTATACG GAGCCCTCAT GTGGTCCCTG 
GGGAAGATCG TGAACACCCC CGAGGTGATC CGGGTCTACA TTGGCTCCTT CTGGTCCCAC 
CCACTCCTCA TTCCCGACAA CCGGAAGCTC TTTGAAGCTG AAGAGCAAGA CTTGTTCAGA 
GACATTCAGA GTCTACCCCG TAATGCTGCT CTTCGAAAGC TCAACGATCT CATCAAGAGA 
GCCCGGCTGG CCAAGGTCCA CGCCTACATC ATCAGCTCCT TGAAGAAGGA GATGCCCTCA 
GTGTTTGGGA AGGACACCAA AAAGAAAGAA CTGGTGAACA ACCTGGCTGA GATCTATGGC 
CGGATTGAGC GAGAACACCA GATCTCCCCT GGAGACTTCC CCAACCTGAA GAGGATGCAG 
GACCAGCTGC AGGCCCAGGA CTTCAGCAAA TTCCAGCCAC TGAAGAGCAA GCTGCTGGAA 
GTGGTTGATG ATATGCTGGC TCATGACATT GCCCAGCTCA TGGTGCTGGT GCGCCAGGAA 
GAGACCCAAC GGCCTGTCCA GATGGTGAAG GGCGGAGCAT TTGAGGGAAC CTTACAAGGC 
CCCTTCGGGC ACGGCTATGG AGAGGGAGCT GGGGAGGGCA TCGATGATGC CGAGTGGGTG 
GTGGCGCGGG ACAAGCCTAT GTATGATGAG ATCTTCTACA CCTTATCCCC AGTGGATGGC 
AAGATCACAG GTGCCAACGC CAAGAAGGAG ATGGTGCGCT CCAAGTTGCC CAACAGCGTG 
CTGGGCAAGA TCTGGAAGCT AGCCGACATT GACAAGGATG GCATGTTGGA TGACGAGGAG 
TTTGCCCTGG CCAACCACCT TATCAAAGTC AAGCTAGAGG GGCATGAGCT GCCCAGTGAG 
CTACCTGCCC ACCTCCTCCC TCCATCTAAG AGGAAAGTAT CAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of the EHD Family of Endocytic Recycling Regulators for TCR Recycling and T Cell Function.
Journal of immunology (Baltimore, Md. : 1950)200(2)483-499(2018 Jan)
Iseka FM,Goetz BT,Mushtaq I,An W,Cypher LR,Bielecki TA,Tom EC,Arya P,Bhattacharyya S,Storck MD,Semerad CL,Talmadge JE,Mosley RL,Band V,Band H

Phosphatidic acid induces EHD3-containing membrane tubulation and is required for receptor recycling.
Experimental cell research342(1)1-10(2016 Mar)
Henmi Y,Oe N,Kono N,Taguchi T,Takei K,Tanabe K

Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.
Scientific reports620727(2016 Feb)
Bhattacharyya S,Rainey MA,Arya P,Dutta S,George M,Storck MD,McComb RD,Muirhead D,Todd GL,Gould K,Datta K,Gelineau-van Waes J,Band V,Band H

The endocytic recycling regulatory protein EHD1 Is required for ocular lens development.
Developmental biology408(1)41-55(2015 Dec)
Arya P,Rainey MA,Bhattacharyya S,Mohapatra BC,George M,Kuracha MR,Storck MD,Band V,Govindarajan V,Band H

SUMOylation of EHD3 Modulates Tubulation of the Endocytic Recycling Compartment.
PloS one10(7)e0134053(2015)
Cabasso O,Pekar O,Horowitz M

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Ehd3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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