Marchf7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Marchf7
Entrez Gene ID	57438
Full Name	membrane-associated ring finger (C3HC4) 7
Synonyms	Axo,Axot,Gtrgeo17
General protein information	Preferred Names membrane-associated ring finger (C3HC4) 7 Names E3 ubiquitin-protein ligase MARCH7 MARCH-VII RING-type E3 ubiquitin transferase MARCH7 axotrophin gene trap ROSA b-geo 17 membrane-associated RING finger protein 7 membrane-associated RING-CH protein VII
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 C1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499952.2	XP_006500015.1	E3 ubiquitin-protein ligase MARCH7 isoform X1
NM_020575.2	NP_065600.1	E3 ubiquitin-protein ligase MARCH7

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MARCH7	NP_001093787.1
	MARCH7	XP_002798930.1
Mus musculus (house mouse)	March7	NP_065600.1
Pan troglodytes (chimpanzee)	MARCH7	XP_515850.3
Gallus gallus (chicken)	MARCH7	XP_004943119.1
Danio rerio (zebrafish)	march7	NP_001108052.2
Rattus norvegicus (Norway rat)	March7	NP_001012087.1
Xenopus tropicalis (tropical clawed frog)	march7	XP_002933327.2
Homo sapiens (human)	MARCH7	NP_073737.1
Canis lupus familiaris (dog)	MARCH7	XP_848724.1

Gene Ontology
Bibliography

Related articles in PubMed

A LIF/Nanog axis is revealed in T lymphocytes that lack MARCH-7, a RINGv E3 ligase that regulates the LIF-receptor.
Thompson LH, Whiston RA, Rakhimov Y, Taccioli C, Liu CG, Croce C, Metcalfe SM
Cell cycle (Georgetown, Tex.)9(20)4213-21(2010 Oct)

A wild derived quantitative trait locus on mouse chromosome 2 prevents obesity.
Mollah MB, Ishikawa A
BMC genetics1184(2010 Sep)

The ubiquitin E3 ligase MARCH7 is differentially regulated by the deubiquitylating enzymes USP7 and USP9X.
Nathan JA, Sengupta S, Wood SA, Admon A, Markson G, Sanderson C, Lehner PJ
Traffic (Copenhagen, Denmark)9(7)1130-45(2008 Jul)

Evidence for functional inter-relationships between FOXP3, leukaemia inhibitory factor, and axotrophin/MARCH-7 in transplantation tolerance.
Muthukumarana PA, Lyons GE, Miura Y, Thompson LH, Watson T, Green CJ, Shurey S, Hess AD, Rosengard BR, Metcalfe SM
International immunopharmacology6(13-14)1993-2001(2006 Dec)

Transcriptome analysis in blastocyst hatching by cDNA microarray.
Chen HW, Chen JJ, Yu SL, Li HN, Yang PC, Su CM, Au HK, Chang CW, Chien LW, Chen CS, Tzeng CR
Human reproduction (Oxford, England)20(9)2492-501(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrate that T cells retain responsiveness to a LIF/Nanog axis and that this axis is linked to MARCH-7, a RINGv E3 ligase that regulates the LIF-receptor.
Title: A LIF/Nanog axis is revealed in T lymphocytes that lack MARCH-7, a RINGv E3 ligase that regulates the LIF-receptor.

Data suggest that thymic Foxp3+T cell development is influenced by axotrophin.
Title: Evidence for functional inter-relationships between FOXP3, leukaemia inhibitory factor, and axotrophin/MARCH-7 in transplantation tolerance.

The following Marchf7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Marchf7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12107	NM_020575.2 Latest version!	Mus musculus membrane associated ring-CH-type finger 7 (Marchf7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu12107	XM_006499952.2 Latest version!	Mus musculus membrane-associated ring finger (C3HC4) 7 (March7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Marchf7 ( NM_020575.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_065600.1 Mus musculus membrane associated ring-CH-type finger 7 (Marchf7), mRNA.

Price & Availability↑

CloneID	OMu12107
Clone ID Related Accession (Same CDS sequence)	XM_006499952.2 , NM_020575.2
Accession Version	NM_020575.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MARCH7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL935326.13. On Nov 17, 2006 this sequence version replaced NM_020575.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF155739.1, AK143177.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCTA AACCTTCCAG GATTCCAAGA AGAATTTCTG TTCAACCCTC TGGCTCTTTA 
AGCACTAGGA TGGTGTCTGG AAACAGAGGA ACCAGTTTAA ATGATTCATA TCATTCTAGA 
GACTCCTCCT TTAGACTGGA TTCTGAATAT CAGTCTGCAT CAGCATCAGC GTGTGCATCA 
CCATGTCAGC CTGCCTGGTA CAGTGAGTCT GAGATACCTC AGGGAGCGCG GGCACGAGCA 
CAGACCCAGC AGCGGGATCA TGACTCAAAG AGACCCAAGC TTTCCTGTAC AAACTGTGCA 
TCTACCTCAG CTGGGAGGAA CGGTGGGAGT GGGTTAAATA CAGTGTCAGA TTCTTCTTGG 
AGGCATAGTC AAGTTCCCAG ATCTTCATCA ATGGTACTTG GTTCATTTGG AACAGACTTG 
ATGAGAGAAA GGAGAGATTT GGACAGGAGA AGAGAGTCCT CCATCAGCAA TCTTATGGAT 
TATAATCACC GAAGTGGTGA TTTCACAACT TCATCATATG TTCAAGAAAG AGTTCCTTCT 
TCATATTCAC AGGGAGCAAG ACCAAAAGAG AATGCAGTGA GCACTTTACA GTTGAATTCA 
TCATCCACCA ATCACCAATT GCCTTCTGAC CATCAGACAG TACCAAGTTC TAGGGACTCC 
AGTAGAAGTT CTTTCAGATC ACATTTTTCT CCAAGACAAT CAGAATCTTT TCGCAACAGT 
TCACATCCTG CATTTTCATA TTTTTCAAGT AGAAATGAAA CTCCAACTAT AAGCAATTCA 
GAAAGGGGTT CATCTCAGAG ACCATATCGA GAATCTTCTG ACAATGAAGG TAGGCGTACA 
ACTAGGAGAT TGCTGTCACG GATAGCTTCT AGCATGTCAT CTACTTTTTT CTCACGAAGA 
TCTAGTCAAG ATTCCTTGAA TACAAGATCT TTGAGTTCTG AAAATTATAT TTCTCCGAGA 
ACCCTGACTT CACAGTCTCG GAATAATGGA ACCTCCTCGT CCTCTGACGT CAGTGAGGGC 
AGGGCAGCTG AAGCATCTCA GGGATTTAGA TTTCTTAGGC GAAGATGGGG GTTGTCGTCG 
CTCAGCCAAA ATCATAGCTC TGAACCAGAG GCAGAAAATT TTAACCAAGA ATCAGAAGGT 
AGAAATTCAG GACCATGGTT GTCTTCTTCA CTTAGAAATA GATGCACACC TTTGTTCTCG 
AGAAGGAGGC GAGAGGGAAG GGATGAGTCT TCAAGAATGT CTACGTCAGA TGTACCACCT 
AGATCTCATA TTTTCAGAAG AGATTCAAAT GAAGTAGTTC ATCTTGAAGC ACAGGGTGAC 
TCCCTTGGGG CTGCTGCCAA CCGACCACAA GCATCTGGAG CGTCAAGCAG TGCTGCTGCA 
GGTGGCTCCA CCCCAGAGTT GCCTCAGGGT GGAAGAAATC CAGGACTAAC AGGGATTCTT 
CCTGGCTCCT TGTTCCGGTT TGCAGTCCCA CCAGCACTCG GCAGTAATCT GGCTGACAAT 
GTCATGATTA CTGTAGATAT TATTCCTTCT GGTTGGAATT CAACTGATGG GAAAAATGAT 
AAAGCTAAAA GTGCACCTTC AAGAGACCCA GAAAAACTTC AGAAAATCAA AGAAAGCCTC 
CTTTTAGAGG ACTCTGATGA TGAAGAAGAA GGGGACTTAT GTAGAATTTG TCAGATGGCA 
GCAGCGTCAT CATCTAATTT ATTGATAGAG CCGTGCAAAT GCACAGGGAG CCTGCAGTAC 
GTCCATCAAG AGTGTATGAA AAAGTGGTTA CAAGCCAAAA TTAATTCTGG CTCTTCATTA 
GAGGCTGTGA CTACCTGTGA ACTCTGTAAA GAGAAGTTGC AACTTAACCT GGAGGATTTT 
GATATTCATG AACTACATAG AGCTCATGCA AATGAACAAG CTGAGTATGA GTTTATCAGC 
TCTGGTCTCT ACCTAGTTGT CTTACTGCAC TTGTGTGAAC AAAGCTTTTC TGATATGATG 
GGAAATACAA TTGAACCAAG CACTCGTGTC CGATTTATTA ACCTTGCAAG AACTCTTCAG 
GCACATATGG AAGATCTCGA AACTTCAGAG GATGAATTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065600.1
CDS	162..2243
Translation	MESKPSRIPRRISVQPSGSLSTRMVSGNRGTSLNDSYHSRDSSFRLDSEYQSASASACASPCQPAWYSESEIPQGARARAQTQQRDHDSKRPKLSCTNCASTSAGRNGGSGLNTVSDSSWRHSQVPRSSSMVLGSFGTDLMRERRDLDRRRESSISNLMDYNHRSGDFTTSSYVQERVPSSYSQGARPKENAVSTLQLNSSSTNHQLPSDHQTVPSSRDSSRSSFRSHFSPRQSESFRNSSHPAFSYFSSRNETPTISNSERGSSQRPYRESSDNEGRRTTRRLLSRIASSMSSTFFSRRSSQDSLNTRSLSSENYISPRTLTSQSRNNGTSSSSDVSEGRAAEASQGFRFLRRRWGLSSLSQNHSSEPEAENFNQESEGRNSGPWLSSSLRNRCTPLFSRRRREGRDESSRMSTSDVPPRSHIFRRDSNEVVHLEAQGDSLGAAANRPQASGASSSAAAGGSTPELPQGGRNPGLTGILPGSLFRFAVPPALGSNLADNVMITVDIIPSGWNSTDGKNDKAKSAPSRDPEKLQKIKESLLLEDSDDEEEGDLCRICQMAAASSSNLLIEPCKCTGSLQYVHQECMKKWLQAKINSGSSLEAVTTCELCKEKLQLNLEDFDIHELHRAHANEQAEYEFISSGLYLVVLLHLCEQSFSDMMGNTIEPSTRVRFINLARTLQAHMEDLETSEDEF

Target ORF information:

RefSeq Version	NM_020575.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane associated ring-CH-type finger 7 (Marchf7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020575.2

ORF Insert Sequence:

ATGGAGTCTA AACCTTCCAG GATTCCAAGA AGAATTTCTG TTCAACCCTC TGGCTCTTTA 
AGCACTAGGA TGGTGTCTGG AAACAGAGGA ACCAGTTTAA ATGATTCATA TCATTCTAGA 
GACTCCTCCT TTAGACTGGA TTCTGAATAT CAGTCTGCAT CAGCATCAGC GTGTGCATCA 
CCATGTCAGC CTGCCTGGTA CAGTGAGTCT GAGATACCTC AGGGAGCGCG GGCACGAGCA 
CAGACCCAGC AGCGGGATCA TGACTCAAAG AGACCCAAGC TTTCCTGTAC AAACTGTGCA 
TCTACCTCAG CTGGGAGGAA CGGTGGGAGT GGGTTAAATA CAGTGTCAGA TTCTTCTTGG 
AGGCATAGTC AAGTTCCCAG ATCTTCATCA ATGGTACTTG GTTCATTTGG AACAGACTTG 
ATGAGAGAAA GGAGAGATTT GGACAGGAGA AGAGAGTCCT CCATCAGCAA TCTTATGGAT 
TATAATCACC GAAGTGGTGA TTTCACAACT TCATCATATG TTCAAGAAAG AGTTCCTTCT 
TCATATTCAC AGGGAGCAAG ACCAAAAGAG AATGCAGTGA GCACTTTACA GTTGAATTCA 
TCATCCACCA ATCACCAATT GCCTTCTGAC CATCAGACAG TACCAAGTTC TAGGGACTCC 
AGTAGAAGTT CTTTCAGATC ACATTTTTCT CCAAGACAAT CAGAATCTTT TCGCAACAGT 
TCACATCCTG CATTTTCATA TTTTTCAAGT AGAAATGAAA CTCCAACTAT AAGCAATTCA 
GAAAGGGGTT CATCTCAGAG ACCATATCGA GAATCTTCTG ACAATGAAGG TAGGCGTACA 
ACTAGGAGAT TGCTGTCACG GATAGCTTCT AGCATGTCAT CTACTTTTTT CTCACGAAGA 
TCTAGTCAAG ATTCCTTGAA TACAAGATCT TTGAGTTCTG AAAATTATAT TTCTCCGAGA 
ACCCTGACTT CACAGTCTCG GAATAATGGA ACCTCCTCGT CCTCTGACGT CAGTGAGGGC 
AGGGCAGCTG AAGCATCTCA GGGATTTAGA TTTCTTAGGC GAAGATGGGG GTTGTCGTCG 
CTCAGCCAAA ATCATAGCTC TGAACCAGAG GCAGAAAATT TTAACCAAGA ATCAGAAGGT 
AGAAATTCAG GACCATGGTT GTCTTCTTCA CTTAGAAATA GATGCACACC TTTGTTCTCG 
AGAAGGAGGC GAGAGGGAAG GGATGAGTCT TCAAGAATGT CTACGTCAGA TGTACCACCT 
AGATCTCATA TTTTCAGAAG AGATTCAAAT GAAGTAGTTC ATCTTGAAGC ACAGGGTGAC 
TCCCTTGGGG CTGCTGCCAA CCGACCACAA GCATCTGGAG CGTCAAGCAG TGCTGCTGCA 
GGTGGCTCCA CCCCAGAGTT GCCTCAGGGT GGAAGAAATC CAGGACTAAC AGGGATTCTT 
CCTGGCTCCT TGTTCCGGTT TGCAGTCCCA CCAGCACTCG GCAGTAATCT GGCTGACAAT 
GTCATGATTA CTGTAGATAT TATTCCTTCT GGTTGGAATT CAACTGATGG GAAAAATGAT 
AAAGCTAAAA GTGCACCTTC AAGAGACCCA GAAAAACTTC AGAAAATCAA AGAAAGCCTC 
CTTTTAGAGG ACTCTGATGA TGAAGAAGAA GGGGACTTAT GTAGAATTTG TCAGATGGCA 
GCAGCGTCAT CATCTAATTT ATTGATAGAG CCGTGCAAAT GCACAGGGAG CCTGCAGTAC 
GTCCATCAAG AGTGTATGAA AAAGTGGTTA CAAGCCAAAA TTAATTCTGG CTCTTCATTA 
GAGGCTGTGA CTACCTGTGA ACTCTGTAAA GAGAAGTTGC AACTTAACCT GGAGGATTTT 
GATATTCATG AACTACATAG AGCTCATGCA AATGAACAAG CTGAGTATGA GTTTATCAGC 
TCTGGTCTCT ACCTAGTTGT CTTACTGCAC TTGTGTGAAC AAAGCTTTTC TGATATGATG 
GGAAATACAA TTGAACCAAG CACTCGTGTC CGATTTATTA ACCTTGCAAG AACTCTTCAG 
GCACATATGG AAGATCTCGA AACTTCAGAG GATGAATTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Marchf7 ( XM_006499952.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500015.1 Mus musculus membrane-associated ring finger (C3HC4) 7 (March7), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu12107
Clone ID Related Accession (Same CDS sequence)	XM_006499952.2 , NM_020575.2
Accession Version	XM_006499952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	E3 ubiquitin-protein ligase MARCH7 isoform X1
Comment

ATGGAGTCTA AACCTTCCAG GATTCCAAGA AGAATTTCTG TTCAACCCTC TGGCTCTTTA 
AGCACTAGGA TGGTGTCTGG AAACAGAGGA ACCAGTTTAA ATGATTCATA TCATTCTAGA 
GACTCCTCCT TTAGACTGGA TTCTGAATAT CAGTCTGCAT CAGCATCAGC GTGTGCATCA 
CCATGTCAGC CTGCCTGGTA CAGTGAGTCT GAGATACCTC AGGGAGCGCG GGCACGAGCA 
CAGACCCAGC AGCGGGATCA TGACTCAAAG AGACCCAAGC TTTCCTGTAC AAACTGTGCA 
TCTACCTCAG CTGGGAGGAA CGGTGGGAGT GGGTTAAATA CAGTGTCAGA TTCTTCTTGG 
AGGCATAGTC AAGTTCCCAG ATCTTCATCA ATGGTACTTG GTTCATTTGG AACAGACTTG 
ATGAGAGAAA GGAGAGATTT GGACAGGAGA AGAGAGTCCT CCATCAGCAA TCTTATGGAT 
TATAATCACC GAAGTGGTGA TTTCACAACT TCATCATATG TTCAAGAAAG AGTTCCTTCT 
TCATATTCAC AGGGAGCAAG ACCAAAAGAG AATGCAGTGA GCACTTTACA GTTGAATTCA 
TCATCCACCA ATCACCAATT GCCTTCTGAC CATCAGACAG TACCAAGTTC TAGGGACTCC 
AGTAGAAGTT CTTTCAGATC ACATTTTTCT CCAAGACAAT CAGAATCTTT TCGCAACAGT 
TCACATCCTG CATTTTCATA TTTTTCAAGT AGAAATGAAA CTCCAACTAT AAGCAATTCA 
GAAAGGGGTT CATCTCAGAG ACCATATCGA GAATCTTCTG ACAATGAAGG TAGGCGTACA 
ACTAGGAGAT TGCTGTCACG GATAGCTTCT AGCATGTCAT CTACTTTTTT CTCACGAAGA 
TCTAGTCAAG ATTCCTTGAA TACAAGATCT TTGAGTTCTG AAAATTATAT TTCTCCGAGA 
ACCCTGACTT CACAGTCTCG GAATAATGGA ACCTCCTCGT CCTCTGACGT CAGTGAGGGC 
AGGGCAGCTG AAGCATCTCA GGGATTTAGA TTTCTTAGGC GAAGATGGGG GTTGTCGTCG 
CTCAGCCAAA ATCATAGCTC TGAACCAGAG GCAGAAAATT TTAACCAAGA ATCAGAAGGT 
AGAAATTCAG GACCATGGTT GTCTTCTTCA CTTAGAAATA GATGCACACC TTTGTTCTCG 
AGAAGGAGGC GAGAGGGAAG GGATGAGTCT TCAAGAATGT CTACGTCAGA TGTACCACCT 
AGATCTCATA TTTTCAGAAG AGATTCAAAT GAAGTAGTTC ATCTTGAAGC ACAGGGTGAC 
TCCCTTGGGG CTGCTGCCAA CCGACCACAA GCATCTGGAG CGTCAAGCAG TGCTGCTGCA 
GGTGGCTCCA CCCCAGAGTT GCCTCAGGGT GGAAGAAATC CAGGACTAAC AGGGATTCTT 
CCTGGCTCCT TGTTCCGGTT TGCAGTCCCA CCAGCACTCG GCAGTAATCT GGCTGACAAT 
GTCATGATTA CTGTAGATAT TATTCCTTCT GGTTGGAATT CAACTGATGG GAAAAATGAT 
AAAGCTAAAA GTGCACCTTC AAGAGACCCA GAAAAACTTC AGAAAATCAA AGAAAGCCTC 
CTTTTAGAGG ACTCTGATGA TGAAGAAGAA GGGGACTTAT GTAGAATTTG TCAGATGGCA 
GCAGCGTCAT CATCTAATTT ATTGATAGAG CCGTGCAAAT GCACAGGGAG CCTGCAGTAC 
GTCCATCAAG AGTGTATGAA AAAGTGGTTA CAAGCCAAAA TTAATTCTGG CTCTTCATTA 
GAGGCTGTGA CTACCTGTGA ACTCTGTAAA GAGAAGTTGC AACTTAACCT GGAGGATTTT 
GATATTCATG AACTACATAG AGCTCATGCA AATGAACAAG CTGAGTATGA GTTTATCAGC 
TCTGGTCTCT ACCTAGTTGT CTTACTGCAC TTGTGTGAAC AAAGCTTTTC TGATATGATG 
GGAAATACAA TTGAACCAAG CACTCGTGTC CGATTTATTA ACCTTGCAAG AACTCTTCAG 
GCACATATGG AAGATCTCGA AACTTCAGAG GATGAATTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006500015.1

Translation

MESKPSRIPRRISVQPSGSLSTRMVSGNRGTSLNDSYHSRDSSFRLDSEYQSASASACASPCQPAWYSESEIPQGARARAQTQQRDHDSKRPKLSCTNCASTSAGRNGGSGLNTVSDSSWRHSQVPRSSSMVLGSFGTDLMRERRDLDRRRESSISNLMDYNHRSGDFTTSSYVQERVPSSYSQGARPKENAVSTLQLNSSSTNHQLPSDHQTVPSSRDSSRSSFRSHFSPRQSESFRNSSHPAFSYFSSRNETPTISNSERGSSQRPYRESSDNEGRRTTRRLLSRIASSMSSTFFSRRSSQDSLNTRSLSSENYISPRTLTSQSRNNGTSSSSDVSEGRAAEASQGFRFLRRRWGLSSLSQNHSSEPEAENFNQESEGRNSGPWLSSSLRNRCTPLFSRRRREGRDESSRMSTSDVPPRSHIFRRDSNEVVHLEAQGDSLGAAANRPQASGASSSAAAGGSTPELPQGGRNPGLTGILPGSLFRFAVPPALGSNLADNVMITVDIIPSGWNSTDGKNDKAKSAPSRDPEKLQKIKESLLLEDSDDEEEGDLCRICQMAAASSSNLLIEPCKCTGSLQYVHQECMKKWLQAKINSGSSLEAVTTCELCKEKLQLNLEDFDIHELHRAHANEQAEYEFISSGLYLVVLLHLCEQSFSDMMGNTIEPSTRVRFINLARTLQAHMEDLETSEDEF

Target ORF information:

RefSeq Version	XM_006499952.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus membrane-associated ring finger (C3HC4) 7 (March7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499952.2

ORF Insert Sequence:

ATGGAGTCTA AACCTTCCAG GATTCCAAGA AGAATTTCTG TTCAACCCTC TGGCTCTTTA 
AGCACTAGGA TGGTGTCTGG AAACAGAGGA ACCAGTTTAA ATGATTCATA TCATTCTAGA 
GACTCCTCCT TTAGACTGGA TTCTGAATAT CAGTCTGCAT CAGCATCAGC GTGTGCATCA 
CCATGTCAGC CTGCCTGGTA CAGTGAGTCT GAGATACCTC AGGGAGCGCG GGCACGAGCA 
CAGACCCAGC AGCGGGATCA TGACTCAAAG AGACCCAAGC TTTCCTGTAC AAACTGTGCA 
TCTACCTCAG CTGGGAGGAA CGGTGGGAGT GGGTTAAATA CAGTGTCAGA TTCTTCTTGG 
AGGCATAGTC AAGTTCCCAG ATCTTCATCA ATGGTACTTG GTTCATTTGG AACAGACTTG 
ATGAGAGAAA GGAGAGATTT GGACAGGAGA AGAGAGTCCT CCATCAGCAA TCTTATGGAT 
TATAATCACC GAAGTGGTGA TTTCACAACT TCATCATATG TTCAAGAAAG AGTTCCTTCT 
TCATATTCAC AGGGAGCAAG ACCAAAAGAG AATGCAGTGA GCACTTTACA GTTGAATTCA 
TCATCCACCA ATCACCAATT GCCTTCTGAC CATCAGACAG TACCAAGTTC TAGGGACTCC 
AGTAGAAGTT CTTTCAGATC ACATTTTTCT CCAAGACAAT CAGAATCTTT TCGCAACAGT 
TCACATCCTG CATTTTCATA TTTTTCAAGT AGAAATGAAA CTCCAACTAT AAGCAATTCA 
GAAAGGGGTT CATCTCAGAG ACCATATCGA GAATCTTCTG ACAATGAAGG TAGGCGTACA 
ACTAGGAGAT TGCTGTCACG GATAGCTTCT AGCATGTCAT CTACTTTTTT CTCACGAAGA 
TCTAGTCAAG ATTCCTTGAA TACAAGATCT TTGAGTTCTG AAAATTATAT TTCTCCGAGA 
ACCCTGACTT CACAGTCTCG GAATAATGGA ACCTCCTCGT CCTCTGACGT CAGTGAGGGC 
AGGGCAGCTG AAGCATCTCA GGGATTTAGA TTTCTTAGGC GAAGATGGGG GTTGTCGTCG 
CTCAGCCAAA ATCATAGCTC TGAACCAGAG GCAGAAAATT TTAACCAAGA ATCAGAAGGT 
AGAAATTCAG GACCATGGTT GTCTTCTTCA CTTAGAAATA GATGCACACC TTTGTTCTCG 
AGAAGGAGGC GAGAGGGAAG GGATGAGTCT TCAAGAATGT CTACGTCAGA TGTACCACCT 
AGATCTCATA TTTTCAGAAG AGATTCAAAT GAAGTAGTTC ATCTTGAAGC ACAGGGTGAC 
TCCCTTGGGG CTGCTGCCAA CCGACCACAA GCATCTGGAG CGTCAAGCAG TGCTGCTGCA 
GGTGGCTCCA CCCCAGAGTT GCCTCAGGGT GGAAGAAATC CAGGACTAAC AGGGATTCTT 
CCTGGCTCCT TGTTCCGGTT TGCAGTCCCA CCAGCACTCG GCAGTAATCT GGCTGACAAT 
GTCATGATTA CTGTAGATAT TATTCCTTCT GGTTGGAATT CAACTGATGG GAAAAATGAT 
AAAGCTAAAA GTGCACCTTC AAGAGACCCA GAAAAACTTC AGAAAATCAA AGAAAGCCTC 
CTTTTAGAGG ACTCTGATGA TGAAGAAGAA GGGGACTTAT GTAGAATTTG TCAGATGGCA 
GCAGCGTCAT CATCTAATTT ATTGATAGAG CCGTGCAAAT GCACAGGGAG CCTGCAGTAC 
GTCCATCAAG AGTGTATGAA AAAGTGGTTA CAAGCCAAAA TTAATTCTGG CTCTTCATTA 
GAGGCTGTGA CTACCTGTGA ACTCTGTAAA GAGAAGTTGC AACTTAACCT GGAGGATTTT 
GATATTCATG AACTACATAG AGCTCATGCA AATGAACAAG CTGAGTATGA GTTTATCAGC 
TCTGGTCTCT ACCTAGTTGT CTTACTGCAC TTGTGTGAAC AAAGCTTTTC TGATATGATG 
GGAAATACAA TTGAACCAAG CACTCGTGTC CGATTTATTA ACCTTGCAAG AACTCTTCAG 
GCACATATGG AAGATCTCGA AACTTCAGAG GATGAATTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A LIF/Nanog axis is revealed in T lymphocytes that lack MARCH-7, a RINGv E3 ligase that regulates the LIF-receptor.
Cell cycle (Georgetown, Tex.)9(20)4213-21(2010 Oct)
Thompson LH,Whiston RA,Rakhimov Y,Taccioli C,Liu CG,Croce C,Metcalfe SM

A wild derived quantitative trait locus on mouse chromosome 2 prevents obesity.
BMC genetics1184(2010 Sep)
Mollah MB,Ishikawa A

The ubiquitin E3 ligase MARCH7 is differentially regulated by the deubiquitylating enzymes USP7 and USP9X.
Traffic (Copenhagen, Denmark)9(7)1130-45(2008 Jul)
Nathan JA,Sengupta S,Wood SA,Admon A,Markson G,Sanderson C,Lehner PJ

Evidence for functional inter-relationships between FOXP3, leukaemia inhibitory factor, and axotrophin/MARCH-7 in transplantation tolerance.
International immunopharmacology6(13-14)1993-2001(2006 Dec)
Muthukumarana PA,Lyons GE,Miura Y,Thompson LH,Watson T,Green CJ,Shurey S,Hess AD,Rosengard BR,Metcalfe SM

Transcriptome analysis in blastocyst hatching by cDNA microarray.
Human reproduction (Oxford, England)20(9)2492-501(2005 Sep)
Chen HW,Chen JJ,Yu SL,Li HN,Yang PC,Su CM,Au HK,Chang CW,Chien LW,Chen CS,Tzeng CR

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Marchf7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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