gRNAs
The following Slc12a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu13016 | |
| Clone ID Related Accession (Same CDS sequence) | NM_020333.2 | |
| Accession Version | NM_020333.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Mus musculus(house mouse) | |
| Product | solute carrier family 12 member 5 isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147262.1 and BC054808.1. On Oct 26, 2007 this sequence version replaced NM_020333.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054808.1, AK147262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCTCAACA ACCTGACGGA CTGCGAGGAC GGCGATGGGG GAGCCAACCC CGGTGATGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_065066.2 |
| CDS | 416..3763 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_020333.2 |
1 | ATGCTCAACA ACCTGACGGA CTGCGAGGAC GGCGATGGGG GAGCCAACCC CGGTGATGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu69716 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006499943.3 , NM_001355480.1 | |
| Accession Version | NM_001355480.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-29 | |
| Organism | Mus musculus(house mouse) | |
| Product | solute carrier family 12 member 5 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from KJ535321.1 and AL591495.12. On Sep 16, 2017 this sequence version replaced XM_006499943.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KJ535321.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG CGTCCGCCGC CTCCGCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001342409.1 |
| CDS | 38..3454 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001355480.1 |
1 | ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG CGTCCGCCGC CTCCGCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu69717 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006499944.3 , NM_001355481.1 | |
| Accession Version | NM_001355481.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-28 | |
| Organism | Mus musculus(house mouse) | |
| Product | solute carrier family 12 member 5 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from KJ535320.1 and AL591495.12. On Sep 16, 2017 this sequence version replaced XM_006499944.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' end compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KJ535320.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG CGTCCGCCGC CTCCGCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001342410.1 |
| CDS | 38..3439 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001355481.1 |
1 | ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG CGTCCGCCGC CTCCGCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu69716 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006499943.3 , NM_001355480.1 | |
| Accession Version | XM_006499943.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(House mouse) | |
| Product | solute carrier family 12 member 5 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499943.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG CGTCCGCCGC CTCCGCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006500006.2 |
| CDS | 63..3479 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006499943.3 |
1 | ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG CGTCCGCCGC CTCCGCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu69717 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006499944.3 , NM_001355481.1 | |
| Accession Version | XM_006499944.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(House mouse) | |
| Product | solute carrier family 12 member 5 isoform X2 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG CGTCCGCCGC CTCCGCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006500007.2 |
| CDS | 63..3464 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006499944.3 |
1 | ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG CGTCCGCCGC CTCCGCTGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
