Insm2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Insm2
Entrez Gene ID	56856
Full Name	insulinoma-associated 2
Synonyms	Mlt1
General protein information	Preferred Names insulinoma-associated 2 Names insulinoma-associated protein 2 methylated in liver tumor 1 mlt 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_020287.2	NP_064683.2	insulinoma-associated protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	INSM2	XP_522825.2
Bos taurus (cattle)	INSM2	NP_001137552.1
Mus musculus (house mouse)	Insm2	NP_064683.2
Danio rerio (zebrafish)	LOC792891	XP_001332514.2
Rattus norvegicus (Norway rat)	Insm2	XP_006240173.1
Homo sapiens (human)	INSM2	NP_115983.3
	INSM2	XP_001088093.1
Canis lupus familiaris (dog)	INSM2	XP_547774.1

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of gene expression rhythms in mice lacking secretory vesicle proteins IA-2 and IA-2?.
Punia S, Rumery KK, Yu EA, Lambert CM, Notkins AL, Weaver DR
American journal of physiology. Endocrinology and metabolism303(6)E762-76(2012 Sep)

Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1.
Cai T, Chen X, Wang R, Xu H, You Y, Zhang T, Lan MS, Notkins AL
Endocrinology152(5)1961-9(2011 May)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Identification of a novel member of the snail/Gfi-1 repressor family, mlt 1, which is methylated and silenced in liver tumors of SV40 T antigen transgenic mice.
Tateno M, Fukunishi Y, Komatsu S, Okazaki Y, Kawai J, Shibata K, Itoh M, Muramatsu M, Held WA, Hayashizaki Y
Cancer research61(3)1144-53(2001 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that IA-2 and IA-2beta play important roles in regulation of circadian rhythms, likely through their participation in neurochemical communication among neurons of suprachiasmatic nucleus.
Title: Disruption of gene expression rhythms in mice lacking secretory vesicle proteins IA-2 and IA-2?.

Results suggest that Insm2 plays an important role in the differentiation cascade of Ngn3/NeuroD1 signaling in pancreatic islets.
Title: Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1.

The following Insm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Insm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58385	NM_020287.2 Latest version!	Mus musculus insulinoma-associated 2 (Insm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$286.30 $409.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Insm2 ( NM_020287.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064683.2 Mus musculus insulinoma-associated 2 (Insm2), mRNA.

Price & Availability↑

CloneID	OMu58385
Clone ID Related Accession (Same CDS sequence)	NM_020287.2
Accession Version	NM_020287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	insulinoma-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158398.2. On Jul 27, 2007 this sequence version replaced NM_020287.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGCCGAGGG GCTTCCTGGT GAAGCGAACC AAGCGCTCCG GCAGCTCCTA CCGCGCGCGC 
CCGGTGGAGC CGCTTTTCCC TCCTCCGGGT CCCCTCGCGG CGCAATCCTC ACCGGAGGAG 
CCCGGTCGGG GACTTTTGGG GTCCCCCTGC CTCGCACCTC CGCAGGACGA CGCCGAGTGG 
GGCGCGGGTG GCGGCGACGG CCCCGGCCCC AGCCCCGCGA GGCCGGCGGG CCCCGAGCTG 
CGCCGCGCGT TCCTGGAGCG CTGCCTGCGC TCTCCGGTCT CTGCGGAGTC CTTCCCCAGC 
GCCACCGCCT TCTGCTCCGC GGCGCCCGCG GCCGTGACCT CCGGGGAGGA GTTGGTACCG 
CCGCAGGTCC CTGTATCCGT TCCAATTCCC GTCCCCGGGC CCGCCCCGCA TGGCCTCCAG 
CGCCGCGGTA AGGGTGCCCC AGTCTGCGCC TCAGCGCCGG CAGCCGTCAG GAAACCTAAG 
GCGGTGAGAA GGCTGAGCTT CGCAGATGAG GTGACCACGT CCCCGGTGCT GGGTCTGAAG 
ATCAAAGAGG AGGAGCCCGG GGCTCCGGCA CGGGCTCTGG GCGGCGTCCG CACCCCGCTG 
GGGGAGTTCA TCTGCCAGCT GTGCAAGCAC CAGTACGCGG ACCCCTTCGC TCTGGCTCAG 
CACCGCTGCT CCCGCATCGT GCGCGTCGAG TACCGCTGTC CCGAGTGCGA CAAGGTCTTC 
AGCTGCCCCG CGAACCTCGC CTCCCACCGC CGCTGGCACA AGCCACGTCC CACGCCTGCC 
TGCGCCGCGA GTAAACCTCC CCACGCGCCT CTGACCCCTC CGGACCCTTC CCTGGCGACG 
GGCAAGGAGA ACGGCCGCGT GCCGCGGACC GACGATCAGC ACCCGCAGGC TCCGGACAGC 
TCCGGAGACG GTCAGCACCG GGACAGCGCC GCGCGCCCGG GCCTACAGGC TCTAGTGTAC 
CCGGAGGCCG CACGACCCCA GGCTCCCTAC CCCGAGGTGA TTCTAGGGCG CCACGGGCCG 
GGGTCGAGCG GTGCCAGCGC AGGAGCGACG TCCGAGGTCT TCGTGTGCCC GTATTGTCAC 
AAAAAGTTCC GTCGCCAAGC CTATCTGCGC AAGCACCTGG GCACCCATGA GACGGGCTCG 
GCCCGTGCAC CAACTCCGGG TTTTGGCTCG GAGCGCACGG CCCCACTCAC CTTTGCGTGC 
CCGCTTTGCG GGGCGCACTT CCCGTCCGCG GACATTCGAG AGAAGCACCG GCTATGGCAC 
GCTGTCCGCG AGGAGCTGCT ACTGCCCGCC TTGGTCGGGG CACCTTCGGA AGCAGGCCCC 
GGAGGGGCAT CCGATGGTAG CGCTCAGCAG ATTTTCTCCT GCAAGTACTG CCCGTCCACT 
TTTTTTAGCT CCCCCGGCTT GACCCGGCAC ATCAATAAGT GCCACCCCTC CGAAAGCCGG 
CAAGTGCTGC TGCTGCAGAT GCCACTGAGG CCTGGCTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064683.2
CDS	557..2038
Translation	MPRGFLVKRTKRSGSSYRARPVEPLFPPPGPLAAQSSPEEPGRGLLGSPCLAPPQDDAEWGAGGGDGPGPSPARPAGPELRRAFLERCLRSPVSAESFPSATAFCSAAPAAVTSGEELVPPQVPVSVPIPVPGPAPHGLQRRGKGAPVCASAPAAVRKPKAVRRLSFADEVTTSPVLGLKIKEEEPGAPARALGGVRTPLGEFICQLCKHQYADPFALAQHRCSRIVRVEYRCPECDKVFSCPANLASHRRWHKPRPTPACAASKPPHAPLTPPDPSLATGKENGRVPRTDDQHPQAPDSSGDGQHRDSAARPGLQALVYPEAARPQAPYPEVILGRHGPGSSGASAGATSEVFVCPYCHKKFRRQAYLRKHLGTHETGSARAPTPGFGSERTAPLTFACPLCGAHFPSADIREKHRLWHAVREELLLPALVGAPSEAGPGGASDGSAQQIFSCKYCPSTFFSSPGLTRHINKCHPSESRQVLLLQMPLRPGC

Target ORF information:

RefSeq Version	NM_020287.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus insulinoma-associated 2 (Insm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020287.2

ORF Insert Sequence:

ATGCCGAGGG GCTTCCTGGT GAAGCGAACC AAGCGCTCCG GCAGCTCCTA CCGCGCGCGC 
CCGGTGGAGC CGCTTTTCCC TCCTCCGGGT CCCCTCGCGG CGCAATCCTC ACCGGAGGAG 
CCCGGTCGGG GACTTTTGGG GTCCCCCTGC CTCGCACCTC CGCAGGACGA CGCCGAGTGG 
GGCGCGGGTG GCGGCGACGG CCCCGGCCCC AGCCCCGCGA GGCCGGCGGG CCCCGAGCTG 
CGCCGCGCGT TCCTGGAGCG CTGCCTGCGC TCTCCGGTCT CTGCGGAGTC CTTCCCCAGC 
GCCACCGCCT TCTGCTCCGC GGCGCCCGCG GCCGTGACCT CCGGGGAGGA GTTGGTACCG 
CCGCAGGTCC CTGTATCCGT TCCAATTCCC GTCCCCGGGC CCGCCCCGCA TGGCCTCCAG 
CGCCGCGGTA AGGGTGCCCC AGTCTGCGCC TCAGCGCCGG CAGCCGTCAG GAAACCTAAG 
GCGGTGAGAA GGCTGAGCTT CGCAGATGAG GTGACCACGT CCCCGGTGCT GGGTCTGAAG 
ATCAAAGAGG AGGAGCCCGG GGCTCCGGCA CGGGCTCTGG GCGGCGTCCG CACCCCGCTG 
GGGGAGTTCA TCTGCCAGCT GTGCAAGCAC CAGTACGCGG ACCCCTTCGC TCTGGCTCAG 
CACCGCTGCT CCCGCATCGT GCGCGTCGAG TACCGCTGTC CCGAGTGCGA CAAGGTCTTC 
AGCTGCCCCG CGAACCTCGC CTCCCACCGC CGCTGGCACA AGCCACGTCC CACGCCTGCC 
TGCGCCGCGA GTAAACCTCC CCACGCGCCT CTGACCCCTC CGGACCCTTC CCTGGCGACG 
GGCAAGGAGA ACGGCCGCGT GCCGCGGACC GACGATCAGC ACCCGCAGGC TCCGGACAGC 
TCCGGAGACG GTCAGCACCG GGACAGCGCC GCGCGCCCGG GCCTACAGGC TCTAGTGTAC 
CCGGAGGCCG CACGACCCCA GGCTCCCTAC CCCGAGGTGA TTCTAGGGCG CCACGGGCCG 
GGGTCGAGCG GTGCCAGCGC AGGAGCGACG TCCGAGGTCT TCGTGTGCCC GTATTGTCAC 
AAAAAGTTCC GTCGCCAAGC CTATCTGCGC AAGCACCTGG GCACCCATGA GACGGGCTCG 
GCCCGTGCAC CAACTCCGGG TTTTGGCTCG GAGCGCACGG CCCCACTCAC CTTTGCGTGC 
CCGCTTTGCG GGGCGCACTT CCCGTCCGCG GACATTCGAG AGAAGCACCG GCTATGGCAC 
GCTGTCCGCG AGGAGCTGCT ACTGCCCGCC TTGGTCGGGG CACCTTCGGA AGCAGGCCCC 
GGAGGGGCAT CCGATGGTAG CGCTCAGCAG ATTTTCTCCT GCAAGTACTG CCCGTCCACT 
TTTTTTAGCT CCCCCGGCTT GACCCGGCAC ATCAATAAGT GCCACCCCTC CGAAAGCCGG 
CAAGTGCTGC TGCTGCAGAT GCCACTGAGG CCTGGCTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disruption of gene expression rhythms in mice lacking secretory vesicle proteins IA-2 and IA-2?.
American journal of physiology. Endocrinology and metabolism303(6)E762-76(2012 Sep)
Punia S,Rumery KK,Yu EA,Lambert CM,Notkins AL,Weaver DR

Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1.
Endocrinology152(5)1961-9(2011 May)
Cai T,Chen X,Wang R,Xu H,You Y,Zhang T,Lan MS,Notkins AL

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Identification of a novel member of the snail/Gfi-1 repressor family, mlt 1, which is methylated and silenced in liver tumors of SV40 T antigen transgenic mice.
Cancer research61(3)1144-53(2001 Feb)
Tateno M,Fukunishi Y,Komatsu S,Okazaki Y,Kawai J,Shibata K,Itoh M,Muramatsu M,Held WA,Hayashizaki Y